Rroxscaffold_2G00152530

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015513
Physical Location & Seq
Forward (+)
89453507 .. 89456492
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152530.1

Sequence Viewer

Length: 1302 bp
ATGGAAACCAGCAAGGCCCAGAAGAAAAAGAAGTGGTTTTTACCACTGGTGCTGTCTCTGCTCCTATCCACTTTCCTCATCTTCATCTCTGTCTTCACTTCAAGCTCATCTGCTTCAAGATTGTACAAGGCCCATGTGAGAAATGACATCCCACATTTCGTGGAGTCAAAGATCAAGGTTTCGGATACTAACAAGGACCCAGTACCCAGATTGGCTTACTTGATCTCCGGCTCAATGGGGGACGGCGAGAGCTTGAAGAGGACACTCAAGGCTCTGTACCATCCAAGGAACCAGTACGCTGTGCACTTGGACCTTGAGGCTTCGGCGGAGGAGAGGTTGGAGTTGGTTAGGTTTGTGAGGAATGAGCCCATGTTTGAGAAGCTGGGGAATGTCAGAGTGGTTGTGAGGGCTAATTTGGTGACCTACAGAGGGCCAACAATGGTGACTAATACGCTTCATGCCGCGGCGATTTTGTTGAAGGAAGGTGGAAAGTGGGATTGGTTTATTAATTTGAGTGCTTCTGATTATCCTTTAGTGACCCAAGATGTTATGGATTGCCTTGATGCATTGGTTTTGCAATCCCATATACTGTTGTTTATAGCCAAGTTGTCACCGATTTTGCTTATTTCCATTTACTTTTATGCATTGGAGTTCTTGGTTACAGCTGGTTTTGATATGCTTCATGTCTTGTCATCCATTCCGAGAACTCTCAACTTTATAGAGCATACCAGTGACATTGGCTGGAAGGAGTATCAGAGGGCAAAACCAGTTATAATTGACCCAGGGCTGTATAGTGTACAAAAGTCAGATGTATTCTGGGTGACAGAGAAAAGGAGCGTTCCAAGTGCGTATAAGCTGTTTACAGGTTCTGCTTGGATGATGCTCTCCCGTCCTTTTGTGGAATACATGGTATGGGGGTGGGACAACCTCCCAAGGATAGTCCTTATGTATTATGCCAACTTTCTTTCTTCACCAGAAGGGTATTTCCACACAGTCATCTGTAATGCTGAGGAGTTTCGAAACACTACTCGCATGGTAGACAGTAATGCACCCTTCGCCAGGAAATTTGGCAGGAACGAGTCGGTTTTAAGCAAGATCGACTCTGAGCTTCTAGGCTGCAATGCTGATGGGTATGTACCAGGAGGATGGCTCAGCACTGGAGCAAATGCAAATGCTACTCTTCCGGATTACACGATAAAAAATGTGACCACTCTTAGGCCAGGTCCTGGTGCTGAAAGACTAAAACATCTCATCACTGGTTTGATATCTGCAGAGGATTTTCATGCAAAGCAATGCACCTGA

Protein Analysis

433

Amino Acids

48.92

Weight (kDa)

8.8

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 71 - 184 1.1e-29 Core-2/I-Branching enzyme
Branch PF02485 225 - 346 8.8e-32 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015513)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03340
malus_domestica MD02G1033100.v1.1 MD15G1173500.v1.1
prunus_persica Prupe.7G242200_v2.0.a1 Prupe.7G242200_v2.0.a1
pyrus_communis pycom02g02720 pycom15g15550
rosa_chinensis RchiOBHm_Chr2g0088581
rosa_laevigata RLG00000015967
rosa_roxburghii Rroxscaffold_2G00152530
rosa_rugosa Rorug01G0485300
rosa_samantha Rh2AG040400 Rh2BG039300 Rh2CG040000 Rh2DG039600
rosa_wichuraiana Rw2G003150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccI GTMKAC 1 cut(s) 1038
AccII CGCG 1 cut(s) 464
AccIII TCCGGA 1 cut(s) 1183
AciI CCGC 3 cut(s) 326, 462, 464
AcsI RAATTY 1 cut(s) 1064
AfaI GTAC 6 cut(s) 125, 204, 278, 296, 798, 1137
AfiI CCNNNNNNNGG 4 cut(s) 429, 1059, 1215, 1226
AgsI TTSAA 4 cut(s) 102, 117, 256, 478
AjnI CCWGG 5 cut(s) 781, 1058, 1138, 1219, 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 481, 513
AluBI AGCT 6 cut(s) 105, 252, 382, 665, 856, 1108
AluI AGCT 6 cut(s) 105, 252, 382, 665, 856, 1108
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 60
Alw44I GTGCAC 1 cut(s) 302
AlwNI CAGNNNCTG 2 cut(s) 869, 1226
Aor13HI TCCGGA 1 cut(s) 1183
AoxI GGCC 4 cut(s) 15, 129, 431, 1217
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 1116
ApoI RAATTY 1 cut(s) 1064
AseI ATTAAT 1 cut(s) 507
AspS9I GGNCC 6 cut(s) 16, 130, 196, 310, 431, 1223
AsuHPI GGTGA 5 cut(s) 430, 454, 603, 832, 963
AsuII TTCGAA 1 cut(s) 1018
AvaII GGWCC 3 cut(s) 196, 310, 1223
BaeGI GKGCMC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 1008
BbvI GCAGC 1 cut(s) 1103
BccI CCATC 3 cut(s) 288, 1121, 1140
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 5 cut(s) 783, 1060, 1140, 1221, 1227
BciVI GTATCC 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 1 cut(s) 1112
BfmI CTRYAG 2 cut(s) 424, 1269
BfuI GTATCC 1 cut(s) 178
BisI GCNGC 3 cut(s) 462, 465, 1117
BlpI GCTNAGC 1 cut(s) 1151
BlsI GCNGC 3 cut(s) 463, 466, 1118
Bme1390I CCNGG 5 cut(s) 783, 1060, 1140, 1221, 1227
Bme18I GGWCC 3 cut(s) 196, 310, 1223
BmgT120I GGNCC 6 cut(s) 16, 130, 196, 310, 431, 1223
BmiI GGNNCC 2 cut(s) 198, 290
BmrFI CCNGG 5 cut(s) 783, 1060, 1140, 1221, 1227
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 553, 870
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 85
BplI GAGNNNNNCTC 2 cut(s) 1134, 1166
BpmI CTGGAG 1 cut(s) 1179
Bpu10I CCTNAGC 1 cut(s) 1008
Bpu1102I GCTNAGC 1 cut(s) 1151
Bpu14I TTCGAA 1 cut(s) 1018
BpuEI CTTGAG 2 cut(s) 251, 335
BsaJI CCNNGG 5 cut(s) 284, 462, 781, 782, 932
BsaWI WCCGGW 1 cut(s) 1183
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 320, 350
Bsc4I CCNNNNNNNGG 4 cut(s) 429, 1059, 1215, 1226
Bse1I ACTGG 7 cut(s) 51, 200, 292, 729, 767, 1162, 1261
Bse3DI GCAATG 2 cut(s) 1126, 1298
BseAI TCCGGA 1 cut(s) 1183
BseBI CCWGG 5 cut(s) 783, 1060, 1140, 1221, 1227
BseDI CCNNGG 5 cut(s) 284, 462, 781, 782, 932
BseGI GGATG 5 cut(s) 147, 280, 692, 882, 1151
BseLI CCNNNNNNNGG 4 cut(s) 429, 1059, 1215, 1226
BseMI GCAATG 2 cut(s) 1126, 1298
BseMII CTCAG 3 cut(s) 999, 1095, 1165
BseNI ACTGG 7 cut(s) 51, 200, 292, 729, 767, 1162, 1261
BseRI GAGGAG 2 cut(s) 344, 1025
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 1103
BseYI CCCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 4 cut(s) 17, 131, 433, 1219
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 2 cut(s) 228, 1184
BslFI GGGAC 2 cut(s) 254, 935
BslI CCNNNNNNNGG 4 cut(s) 429, 1059, 1215, 1226
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 2 cut(s) 254, 935
BsnI GGCC 4 cut(s) 17, 131, 433, 1219
Bsp119I TTCGAA 1 cut(s) 1018
Bsp1286I GDGCHC 2 cut(s) 306, 369
Bsp13I TCCGGA 1 cut(s) 1183
Bsp1407I TGTACA 2 cut(s) 123, 796
Bsp143I GATC 3 cut(s) 171, 222, 1095
Bsp1720I GCTNAGC 1 cut(s) 1151
BspACI CCGC 3 cut(s) 326, 462, 464
BspANI GGCC 4 cut(s) 17, 131, 433, 1219
BspCNI CTCAG 3 cut(s) 1000, 1096, 1164
BspEI TCCGGA 1 cut(s) 1183
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 2 cut(s) 198, 290
BspMAI CTGCAG 1 cut(s) 1273
BspT104I TTCGAA 1 cut(s) 1018
BsrDI GCAATG 2 cut(s) 1126, 1298
BsrGI TGTACA 2 cut(s) 123, 796
BsrI ACTGG 7 cut(s) 51, 200, 292, 729, 767, 1162, 1261
BssECI CCNNGG 5 cut(s) 284, 462, 781, 782, 932
BssMI GATC 3 cut(s) 171, 222, 1095
BssT1I CCWWGG 2 cut(s) 284, 932
Bst2UI CCWGG 5 cut(s) 783, 1060, 1140, 1221, 1227
Bst4CI ACNGT 3 cut(s) 591, 994, 1043
Bst6I CTCTTC 2 cut(s) 251, 1185
BstAUI TGTACA 2 cut(s) 123, 796
BstBI TTCGAA 1 cut(s) 1018
BstDEI CTNAG 4 cut(s) 1008, 1104, 1151, 1214
BstDSI CCRYGG 1 cut(s) 462
BstEII GGTNACC 1 cut(s) 418
BstENI CCTNNNNNAGG 1 cut(s) 1057
BstF5I GGATG 5 cut(s) 147, 280, 692, 882, 1151
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 3 cut(s) 174, 225, 1098
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 3 cut(s) 171, 222, 1095
BstMWI GCNNNNNNNGC 2 cut(s) 58, 1055
BstNI CCWGG 5 cut(s) 783, 1060, 1140, 1221, 1227
BstPI GGTNACC 1 cut(s) 418
BstSCI CCNGG 5 cut(s) 781, 1058, 1138, 1219, 1225
BstSFI CTRYAG 2 cut(s) 424, 1269
BstSLI GKGCMC 1 cut(s) 306
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 1103
BstV2I GAAGAC 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 1146
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 4 cut(s) 17, 131, 433, 1219
BtgI CCRYGG 1 cut(s) 462
BtsCI GGATG 5 cut(s) 147, 280, 692, 882, 1151
BtsIMutI CAGTG 4 cut(s) 44, 736, 1155, 1254
CaiI CAGNNNCTG 2 cut(s) 869, 1226
Cfr13I GGNCC 6 cut(s) 16, 130, 196, 310, 431, 1223
Cfr42I CCGCGG 1 cut(s) 465
Csp6I GTAC 6 cut(s) 124, 203, 277, 295, 797, 1136
CviAII CATG 7 cut(s) 134, 370, 458, 683, 907, 1033, 1283
CviQI GTAC 6 cut(s) 124, 203, 277, 295, 797, 1136
DdeI CTNAG 4 cut(s) 1008, 1104, 1151, 1214
DpnI GATC 3 cut(s) 173, 224, 1097
DpnII GATC 3 cut(s) 171, 222, 1095
Eam1104I CTCTTC 2 cut(s) 251, 1185
EarI CTCTTC 2 cut(s) 251, 1185
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 2 cut(s) 284, 932
Eco24I GRGCYC 1 cut(s) 369
Eco32I GATATC 1 cut(s) 1266
Eco47I GGWCC 3 cut(s) 196, 310, 1223
Eco91I GGTNACC 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 1057
EcoO109I RGGNCCY 2 cut(s) 196, 1223
EcoO65I GGTNACC 1 cut(s) 418
EcoRII CCWGG 5 cut(s) 781, 1058, 1138, 1219, 1225
EcoRV GATATC 1 cut(s) 1266
EcoT14I CCWWGG 2 cut(s) 284, 932
EcoT22I ATGCAT 2 cut(s) 568, 646
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 2 cut(s) 284, 932
FaeI CATG 7 cut(s) 137, 373, 461, 686, 910, 1036, 1286
FaqI GGGAC 2 cut(s) 254, 935
FatI CATG 7 cut(s) 133, 369, 457, 682, 906, 1032, 1282
FblI GTMKAC 1 cut(s) 1038
Fnu4HI GCNGC 3 cut(s) 462, 465, 1117
FokI GGATG 5 cut(s) 134, 267, 679, 889, 1158
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 3 cut(s) 462, 465, 1117
FspBI CTAG 1 cut(s) 1112
GluI GCNGC 3 cut(s) 462, 465, 1117
GsaI CCCAGC 1 cut(s) 386
GsuI CTGGAG 1 cut(s) 1179
HaeIII GGCC 4 cut(s) 17, 131, 433, 1219
HapII CCGG 2 cut(s) 228, 1184
Hin1II CATG 7 cut(s) 137, 373, 461, 686, 910, 1036, 1286
HinfI GANTC 3 cut(s) 164, 1079, 1100
HpaII CCGG 2 cut(s) 228, 1184
HphI GGTGA 5 cut(s) 430, 454, 603, 832, 963
Hpy166II GTNNAC 4 cut(s) 304, 797, 861, 1039
Hpy188I TCNGA 7 cut(s) 184, 395, 523, 702, 756, 808, 1105
Hpy188III TCNNGA 2 cut(s) 117, 1184
Hpy8I GTNNAC 4 cut(s) 304, 797, 861, 1039
HpyAV CCTTC 5 cut(s) 472, 476, 739, 971, 1063
HpyCH4III ACNGT 3 cut(s) 591, 994, 1043
HpyF10VI GCNNNNNNNGC 2 cut(s) 58, 1055
HpyF3I CTNAG 4 cut(s) 1008, 1104, 1151, 1214
Hsp92II CATG 7 cut(s) 137, 373, 461, 686, 910, 1036, 1286
Kpn2I TCCGGA 1 cut(s) 1183
KspI CCGCGG 1 cut(s) 465
Kzo9I GATC 3 cut(s) 171, 222, 1095
LmnI GCTCC 3 cut(s) 66, 834, 1160
Lsp1109I GCAGC 1 cut(s) 1103
LweI GCATC 2 cut(s) 553, 870
MaeI CTAG 1 cut(s) 1112
MaeIII GTNAC 8 cut(s) 418, 442, 535, 609, 658, 731, 820, 1204
MalI GATC 3 cut(s) 173, 224, 1097
MboI GATC 3 cut(s) 171, 222, 1095
MboII GAAGA 6 cut(s) 34, 73, 85, 268, 960, 1172
MhlI GDGCHC 2 cut(s) 306, 369
MluCI AATT 4 cut(s) 412, 508, 774, 1064
MlyI GAGTC 3 cut(s) 173, 1088, 1094
MmeI TCCRAC 1 cut(s) 318
Mph1103I ATGCAT 2 cut(s) 568, 646
MroI TCCGGA 1 cut(s) 1183
MseI TTAA 2 cut(s) 507, 1088
MslI CAYNNNNRTG 1 cut(s) 729
MspA1I CMGCKG 2 cut(s) 464, 665
MspI CCGG 2 cut(s) 228, 1184
MspR9I CCNGG 5 cut(s) 783, 1060, 1140, 1221, 1227
MvaI CCWGG 5 cut(s) 783, 1060, 1140, 1221, 1227
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 2 cut(s) 58, 1055
NdeII GATC 3 cut(s) 171, 222, 1095
NlaIII CATG 7 cut(s) 137, 373, 461, 686, 910, 1036, 1286
NlaIV GGNNCC 2 cut(s) 198, 290
NmuCI GTSAC 7 cut(s) 418, 442, 535, 609, 731, 820, 1204
NsiI ATGCAT 2 cut(s) 568, 646
NspV TTCGAA 1 cut(s) 1018
PasI CCCWGGG 1 cut(s) 782
PflMI CCANNNNNTGG 1 cut(s) 1226
PkrI GCNGC 3 cut(s) 463, 466, 1118
PleI GAGTC 3 cut(s) 172, 1087, 1094
PpsI GAGTC 3 cut(s) 172, 1087, 1094
PpuMI RGGWCCY 2 cut(s) 196, 1223
PshBI ATTAAT 1 cut(s) 507
PsiI TTATAA 1 cut(s) 773
Psp5II RGGWCCY 2 cut(s) 196, 1223
Psp6I CCWGG 5 cut(s) 781, 1058, 1138, 1219, 1225
PspEI GGTNACC 1 cut(s) 418
PspFI CCCAGC 1 cut(s) 382
PspGI CCWGG 5 cut(s) 781, 1058, 1138, 1219, 1225
PspN4I GGNNCC 2 cut(s) 198, 290
PspPI GGNCC 6 cut(s) 16, 130, 196, 310, 431, 1223
PspPPI RGGWCCY 2 cut(s) 196, 1223
PstI CTGCAG 1 cut(s) 1273
PstNI CAGNNNCTG 2 cut(s) 869, 1226
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 6 cut(s) 125, 204, 278, 296, 798, 1137
RsaNI GTAC 6 cut(s) 124, 203, 277, 295, 797, 1136
RseI CAYNNNNRTG 1 cut(s) 729
SacII CCGCGG 1 cut(s) 465
SaqAI TTAA 2 cut(s) 507, 1088
SatI GCNGC 3 cut(s) 462, 465, 1117
Sau3AI GATC 3 cut(s) 171, 222, 1095
Sau96I GGNCC 6 cut(s) 16, 130, 196, 310, 431, 1223
SchI GAGTC 3 cut(s) 173, 1088, 1094
ScrFI CCNGG 5 cut(s) 783, 1060, 1140, 1221, 1227
SduI GDGCHC 2 cut(s) 306, 369
SfaNI GCATC 2 cut(s) 553, 870
SfcI CTRYAG 2 cut(s) 424, 1269
Sfr303I CCGCGG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 1018
SgrBI CCGCGG 1 cut(s) 465
SinI GGWCC 3 cut(s) 196, 310, 1223
SmiMI CAYNNNNRTG 1 cut(s) 729
SmlI CTYRAG 2 cut(s) 266, 314
SmoI CTYRAG 2 cut(s) 266, 314
Sse9I AATT 4 cut(s) 412, 508, 774, 1064
SsiI CCGC 3 cut(s) 326, 462, 464
SspMI CTAG 1 cut(s) 1112
StyD4I CCNGG 5 cut(s) 781, 1058, 1138, 1219, 1225
StyI CCWWGG 2 cut(s) 284, 932
TaaI ACNGT 3 cut(s) 591, 994, 1043
TaqI TCGA 2 cut(s) 1018, 1098
TasI AATT 4 cut(s) 412, 508, 774, 1064
TatI WGTACW 2 cut(s) 123, 796
TauI GCSGC 2 cut(s) 464, 467
Tru1I TTAA 2 cut(s) 507, 1088
Tru9I TTAA 2 cut(s) 507, 1088
TscAI CASTG 4 cut(s) 51, 736, 1162, 1261
TseFI GTSAC 7 cut(s) 418, 442, 535, 609, 731, 820, 1204
TseI GCWGC 1 cut(s) 1116
Tsp45I GTSAC 7 cut(s) 418, 442, 535, 609, 731, 820, 1204
TspDTI ATGAA 4 cut(s) 73, 446, 671, 1271
TspRI CASTG 4 cut(s) 51, 736, 1162, 1261
Van91I CCANNNNNTGG 1 cut(s) 1226
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 196, 310, 1223
VspI ATTAAT 1 cut(s) 507
XagI CCTNNNNNAGG 1 cut(s) 1057
XapI RAATTY 1 cut(s) 1064
XmiI GTMKAC 1 cut(s) 1038
XspI CTAG 1 cut(s) 1112
Zsp2I ATGCAT 2 cut(s) 568, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.