Rroxscaffold_3G00252290

protein DJ-1 homolog D-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0020364
Physical Location & Seq
Forward (+)
46321508 .. 46322238
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252290.1

Sequence Viewer

Length: 324 bp
ATGACCAGAATCCGGCGACCGGTGACCAGCTCCGGTAAAGTCTCCTATGGTTTCTCTCTCTCTCTAAGTAACAAAGGGGACTTCATGGAAGATTATGAGATAATCGTGCCTTTTCAGTCACTTCAAGCACTTGGATGCCATGTTGATGAAGTTTGCCCCAAGAGGAAAGTCCGGCGATATGCCACAAGGTACGATGCTCTTGTAGTTCCCGGAGGTCGTGCCCTAGAGTATCCGGCATTGAATGAGAGGGTTATTGCATTAGTGAAACATTTTATGGGAGCAAGGAAACCAATTGGATCCATTTGCCATGGTCGCAGATTTTAG

Protein Analysis

107

Amino Acids

12.09

Weight (kDa)

9.95

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DJ-1_PfpI PF01965 62 - 105 1.3e-10 DJ-1/PfpI family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020364)

Species Orthologous Gene IDs
malus_domestica MD09G1281700.v1.1
rosa_chinensis RchiOBHm_Chr3g0487841
rosa_roxburghii Rroxscaffold_3G00252290 Rroxscaffold_6G00394410
rosa_samantha Rh3BG322100 Rh3CG320400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 291, 304
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 2 cut(s) 12, 19
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 2 cut(s) 125, 241
AluBI AGCT 1 cut(s) 30
AluI AGCT 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 2 cut(s) 291, 304
AsiGI ACCGGT 1 cut(s) 19
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 223
BamHI GGATCC 1 cut(s) 296
BciVI GTATCC 1 cut(s) 240
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 224
BfuI GTATCC 1 cut(s) 240
Bme1390I CCNGG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 2 cut(s) 125, 184
BpuMI CCSGG 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 307
BsaWI WCCGGW 2 cut(s) 19, 32
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 19
Bse118I RCCGGY 1 cut(s) 19
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 12, 19
BseSI GKGCMC 1 cut(s) 223
Bsh1285I CGRYCG 1 cut(s) 20
BshTI ACCGGT 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 20
BsiSI CCGG 6 cut(s) 13, 20, 33, 172, 210, 233
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 12, 19
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 296
Bsp19I CCATGG 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 2 cut(s) 291, 304
BsrFI RCCGGY 1 cut(s) 19
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 307
BstDEI CTNAG 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 307
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 1 cut(s) 299
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 1 cut(s) 296
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BsuI GTATCC 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 1 cut(s) 140
Cfr10I RCCGGY 1 cut(s) 19
Csp6I GTAC 1 cut(s) 190
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 3 cut(s) 85, 140, 308
CviJI RGCY 1 cut(s) 30
CviKI_1 RGCY 1 cut(s) 30
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 1 cut(s) 65
DpnI GATC 1 cut(s) 298
DpnII GATC 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 307
Eco91I GGTNACC 1 cut(s) 22
EcoO65I GGTNACC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 3 cut(s) 88, 143, 311
FaiI YATR 7 cut(s) 48, 86, 96, 141, 180, 275, 309
FaqI GGGAC 1 cut(s) 92
FatI CATG 3 cut(s) 84, 139, 307
FokI GGATG 1 cut(s) 147
FspBI CTAG 1 cut(s) 224
HapII CCGG 6 cut(s) 13, 20, 33, 172, 210, 233
Hin1II CATG 3 cut(s) 88, 143, 311
HinfI GANTC 1 cut(s) 9
HpaII CCGG 6 cut(s) 13, 20, 33, 172, 210, 233
HphI GGTGA 1 cut(s) 34
HpyCH4V TGCA 1 cut(s) 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 1 cut(s) 65
Hsp92II CATG 3 cut(s) 88, 143, 311
Kzo9I GATC 1 cut(s) 296
LmnI GCTCC 2 cut(s) 35, 278
LpnPI CCDG 8 cut(s) 19, 26, 33, 40, 46, 185, 223, 246
LweI GCATC 2 cut(s) 125, 184
MaeI CTAG 1 cut(s) 224
MaeIII GTNAC 3 cut(s) 22, 68, 117
MalI GATC 1 cut(s) 298
MboI GATC 1 cut(s) 296
MboII GAAGA 1 cut(s) 101
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 291
MnlI CCTC 3 cut(s) 156, 206, 240
MslI CAYNNNNRTG 2 cut(s) 133, 144
MspI CCGG 6 cut(s) 13, 20, 33, 172, 210, 233
MspR9I CCNGG 1 cut(s) 210
MunI CAATTG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 210
NcoI CCATGG 1 cut(s) 307
NdeII GATC 1 cut(s) 296
NlaIII CATG 3 cut(s) 88, 143, 311
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 22, 117
PfeI GAWTC 1 cut(s) 9
PfoI TCCNGGA 1 cut(s) 208
PinAI ACCGGT 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 298
PsuI RGATCY 1 cut(s) 296
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 133, 144
Sau3AI GATC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 223
SetI ASST 3 cut(s) 32, 191, 217
SfaNI GCATC 2 cut(s) 125, 184
SmiMI CAYNNNNRTG 2 cut(s) 133, 144
Sse9I AATT 1 cut(s) 291
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 307
TasI AATT 1 cut(s) 291
TfiI GAWTC 1 cut(s) 9
TseFI GTSAC 2 cut(s) 22, 117
Tsp45I GTSAC 2 cut(s) 22, 117
TspDTI ATGAA 2 cut(s) 73, 162
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.