Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0018636

Physical Location & Seq

Genomic Coordinates Reverse (-)

58469113 .. 58471936

Gene Structure 2824 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00265010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1197 bp

ATGAATTCTCCATCTGTGAGGGAGAGGACCACAAAAAGGCCCAAGGTAGAAGAAGAGATTAATGATCATCATCTGGGAACAACAAGCATGCATCAGATAGAGATCAAAGATACTTGTGCTTCTTGTTTGTGTGATGATTGTGCCAACTTTGCTGTGACATTCAGTTTTAAATATAACGATGGTGAAATTGAGCTTTTACGCAAGGTCGCCACTCACGAAATACTCCACCGCAGAAAACTCCAGTTGGTACTCAATCTCAATCACACCTTGCTGAGAGTCGCCGGTATAGATGATCTGACCCTGGAAGAAGAAGCATATTTAGATACCCAATTCACCGATGATCCTCTGCGGCAAGATATTCTCATAGTGACAGTTCCTCCCAAAATGAAATCTATGCTTCTCAAGTTTAGGCCCTATGTTCAAACCTTTCTCAAGGAAGCCAGTGACATGTTTGAGATGAATGTGTACTCAAATTCCCCTCTATCTCTTGTGCGGGAAATGATTCCGCTACTGGATCCAAGAAATGAGTACTTCGGTTCTAGGGTGTTTTCGCGAGACGATTTTTGCAGAACCCACCCGAAAAACAAGTGCCTCGATGTTTTGCTTGCTCAAGAGTCTGCTGTTTTGATCCTTGATCATAAACTAGAGGCATGGAGGGAGCAAAATCAAAATAATGTAATGCCTATGCAAAAGTATTTTTTCTGTGAAGAAGAGGAGAGCGAGCTCAGGTCTTGTTGCGAGCGGGAAACTAATGAAGGGTATTATCTTGCATTTATTCTTCAACGTCTTAAGCAGATACACAATAGGTTCTTTGATGACGAAGTAATATGTGACAAACTTATCGACAGAGATGTGAGGAAGGTTTTAGAAACTCTTCGGGGGTCCGTCTTGGGAGGGTGTAAAATTGCTTTCGACTCTCTTCAGGCCGATAAGCTACAGTTGTGGAAGATGGCTGAGCAGTTGGGAGCTACTTGTGTTGAACAAGTGGACGCATCGGTCACACATGTGGTTGCAGCTCATGCTGGAACACCGGAATCTCTTTGGGCACTAGAAGAGAAAAAGTTCTTGGTCTATCCGAGTTGGATTGAAGCATCACACTTGTCCTCAAGAGTCAAGGCTCAAGCAGAGTTCTGGGTTCTTGAAGGCTTGAAGGCTGGGTTCAATCGATACTTCGATTTCAGATTCTTGAAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008022 GO:0008150 GO:0008152 GO:0008420 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070940 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT NIF PTCB-BRCT

Protein Analysis

398

Amino Acids

46.21

Weight (kDa)

5.56

Isoelectric Point (pI)

48.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	82 - 246	2.9e-20	NLI interacting factor-like phosphatase
BRCT	PF00533	296 - 365	2.4e-07	BRCA1 C Terminus (BRCT) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018636)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr7g0189961
rosa_laevigata	RLG00000004573 RLG00000024741
rosa_multiflora	Rmu_co8447775.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265010
rosa_rugosa	Rorug06G0501500
rosa_samantha	Rh7AG106600 Rh7BG109300 Rh7CG111400 Rh7DG109800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	995
AccBSI	CCGCTC	1 cut(s)	742
AccII	CGCG	1 cut(s)	553
AciI	CCGC	5 cut(s)	229, 349, 493, 506, 742
AclWI	GGATC	4 cut(s)	335, 509, 522, 622
AcsI	RAATTY	2 cut(s)	4, 472
AcuI	CTGAAG	1 cut(s)	905
AfaI	GTAC	3 cut(s)	249, 467, 530
AfiI	CCNNNNNNNGG	1 cut(s)	36
AflII	CTTAAG	1 cut(s)	788
AflIII	ACRYGT	2 cut(s)	447, 1003
AgsI	TTSAA	8 cut(s)	422, 782, 980, 1088, 1142, 1150, 1162, 1189
AjnI	CCWGG	1 cut(s)	300
AjuI	GAANNNNNNNTTGG	2 cut(s)	1049, 1081
AleI	CACNNNNGTG	1 cut(s)	1004
AluBI	AGCT	5 cut(s)	193, 724, 934, 968, 1016
AluI	AGCT	5 cut(s)	193, 724, 934, 968, 1016
Alw21I	GWGCWC	1 cut(s)	726
Alw26I	GTCTC	1 cut(s)	549
AlwI	GGATC	4 cut(s)	335, 509, 522, 622
AoxI	GGCC	3 cut(s)	38, 410, 924
ApeKI	GCWGC	1 cut(s)	1013
ApoI	RAATTY	2 cut(s)	4, 472
AseI	ATTAAT	1 cut(s)	60
Asp700I	GAANNNNTTC	3 cut(s)	501, 873, 1061
AspS9I	GGNCC	4 cut(s)	27, 39, 411, 882
AsuHPI	GGTGA	2 cut(s)	194, 325
AvaII	GGWCC	2 cut(s)	27, 882
BaeGI	GKGCMC	1 cut(s)	1048
BamHI	GGATCC	1 cut(s)	514
BanII	GRGCYC	1 cut(s)	726
Bbv12I	GWGCWC	1 cut(s)	726
BbvI	GCAGC	1 cut(s)	1025
BccI	CCATC	3 cut(s)	19, 173, 943
BciT130I	CCWGG	1 cut(s)	302
BclI	TGATCA	2 cut(s)	64, 634
BcoDI	GTCTC	1 cut(s)	549
BfaI	CTAG	3 cut(s)	540, 644, 1049
BfmI	CTRYAG	1 cut(s)	935
BfrI	CTTAAG	1 cut(s)	788
BisI	GCNGC	2 cut(s)	350, 1014
BlpI	GCTNAGC	1 cut(s)	954
BlsI	GCNGC	2 cut(s)	351, 1015
BmcAI	AGTACT	1 cut(s)	530
Bme1390I	CCNGG	1 cut(s)	302
Bme18I	GGWCC	2 cut(s)	27, 882
BmgT120I	GGNCC	4 cut(s)	27, 39, 411, 882
BmiI	GGNNCC	2 cut(s)	516, 883
BmrFI	CCNGG	1 cut(s)	302
BmsI	GCATC	3 cut(s)	100, 1001, 1100
BpmI	CTGGAG	1 cut(s)	224
Bpu10I	CCTNAGC	1 cut(s)	725
Bpu1102I	GCTNAGC	1 cut(s)	954
BpuEI	CTTGAG	5 cut(s)	386, 416, 594, 1090, 1104
Bsa29I	ATCGAT	1 cut(s)	1165
BsaBI	GATNNNNATC	2 cut(s)	69, 101
BsaJI	CCNNGG	2 cut(s)	42, 300
BsaWI	WCCGGW	1 cut(s)	1030
BsaXI	ACNNNNNCTCC	2 cut(s)	361, 391
Bsc4I	CCNNNNNNNGG	1 cut(s)	36
Bse118I	RCCGGY	1 cut(s)	281
Bse1I	ACTGG	3 cut(s)	241, 441, 516
Bse8I	GATNNNNATC	2 cut(s)	69, 101
BseBI	CCWGG	1 cut(s)	302
BseCI	ATCGAT	1 cut(s)	1165
BseDI	CCNNGG	2 cut(s)	42, 300
BseJI	GATNNNNATC	2 cut(s)	69, 101
BseLI	CCNNNNNNNGG	1 cut(s)	36
BseMII	CTCAG	3 cut(s)	263, 739, 945
BseNI	ACTGG	3 cut(s)	241, 441, 516
BseRI	GAGGAG	1 cut(s)	728
BseSI	GKGCMC	1 cut(s)	1048
BseXI	GCAGC	1 cut(s)	1025
BseYI	CCCAGC	1 cut(s)	1154
Bsh1236I	CGCG	1 cut(s)	553
BshFI	GGCC	3 cut(s)	40, 412, 926
BshVI	ATCGAT	1 cut(s)	1165
BsiHKAI	GWGCWC	1 cut(s)	726
BsiSI	CCGG	2 cut(s)	282, 1031
BslI	CCNNNNNNNGG	1 cut(s)	36
BsmAI	GTCTC	1 cut(s)	549
BsmBI	CGTCTC	1 cut(s)	549
BsnI	GGCC	3 cut(s)	40, 412, 926
Bsp1286I	GDGCHC	2 cut(s)	726, 1048
Bsp143I	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
Bsp1720I	GCTNAGC	1 cut(s)	954
Bsp68I	TCGCGA	1 cut(s)	553
BspACI	CCGC	5 cut(s)	229, 349, 493, 506, 742
BspANI	GGCC	3 cut(s)	40, 412, 926
BspCNI	CTCAG	3 cut(s)	264, 738, 946
BspDI	ATCGAT	1 cut(s)	1165
BspFNI	CGCG	1 cut(s)	553
BspLI	GGNNCC	2 cut(s)	516, 883
BspPI	GGATC	4 cut(s)	335, 509, 522, 622
BspTI	CTTAAG	1 cut(s)	788
BsrBI	CCGCTC	1 cut(s)	742
BsrFI	RCCGGY	1 cut(s)	281
BsrI	ACTGG	3 cut(s)	241, 441, 516
BssAI	RCCGGY	1 cut(s)	281
BssECI	CCNNGG	2 cut(s)	42, 300
BssMI	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
BssT1I	CCWWGG	1 cut(s)	42
Bst2UI	CCWGG	1 cut(s)	302
Bst4CI	ACNGT	2 cut(s)	373, 939
Bst6I	CTCTTC	5 cut(s)	48, 705, 879, 924, 1047
BstAFI	CTTAAG	1 cut(s)	788
BstAPI	GCANNNNNTGC	1 cut(s)	1019
BstC8I	GCNNGC	4 cut(s)	89, 606, 722, 740
BstDEI	CTNAG	3 cut(s)	272, 725, 954
BstFNI	CGCG	1 cut(s)	553
BstKTI	GATC	7 cut(s)	67, 105, 295, 343, 517, 630, 637
BstMAI	GTCTC	1 cut(s)	549
BstMBI	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
BstMWI	GCNNNNNNNGC	2 cut(s)	149, 1019
BstNI	CCWGG	1 cut(s)	302
BstNSI	RCATGY	3 cut(s)	91, 451, 1007
BstSCI	CCNGG	1 cut(s)	300
BstSFI	CTRYAG	1 cut(s)	935
BstSLI	GKGCMC	1 cut(s)	1048
BstUI	CGCG	1 cut(s)	553
BstV1I	GCAGC	1 cut(s)	1025
BstX2I	RGATCY	1 cut(s)	514
BstYI	RGATCY	1 cut(s)	514
Bsu15I	ATCGAT	1 cut(s)	1165
BsuRI	GGCC	3 cut(s)	40, 412, 926
BsuTUI	ATCGAT	1 cut(s)	1165
BtsIMutI	CAGTG	1 cut(s)	448
BtuMI	TCGCGA	1 cut(s)	553
Cac8I	GCNNGC	4 cut(s)	89, 606, 722, 740
Cfr10I	RCCGGY	1 cut(s)	281
Cfr13I	GGNCC	4 cut(s)	27, 39, 411, 882
ClaI	ATCGAT	1 cut(s)	1165
CseI	GACGC	1 cut(s)	998
Csp6I	GTAC	3 cut(s)	248, 466, 529
CviAII	CATG	5 cut(s)	88, 448, 651, 1004, 1019
CviQI	GTAC	3 cut(s)	248, 466, 529
DdeI	CTNAG	3 cut(s)	272, 725, 954
DpnI	GATC	7 cut(s)	66, 104, 294, 342, 516, 629, 636
DpnII	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
DraI	TTTAAA	1 cut(s)	169
DrdI	GACNNNNNNGTC	1 cut(s)	995
DseDI	GACNNNNNNGTC	1 cut(s)	995
Eam1104I	CTCTTC	5 cut(s)	48, 705, 879, 924, 1047
EarI	CTCTTC	5 cut(s)	48, 705, 879, 924, 1047
Ecl136II	GAGCTC	1 cut(s)	724
Eco130I	CCWWGG	1 cut(s)	42
Eco24I	GRGCYC	1 cut(s)	726
Eco47I	GGWCC	2 cut(s)	27, 882
Eco53kI	GAGCTC	1 cut(s)	724
Eco57I	CTGAAG	1 cut(s)	905
EcoICRI	GAGCTC	1 cut(s)	724
EcoO109I	RGGNCCY	1 cut(s)	411
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	1 cut(s)	300
EcoT14I	CCWWGG	1 cut(s)	42
EcoT22I	ATGCAT	1 cut(s)	93
EcoT38I	GRGCYC	1 cut(s)	726
ErhI	CCWWGG	1 cut(s)	42
Esp3I	CGTCTC	1 cut(s)	549
FaeI	CATG	5 cut(s)	91, 451, 654, 1007, 1022
FatI	CATG	5 cut(s)	87, 447, 650, 1003, 1018
FauI	CCCGC	2 cut(s)	486, 735
FbaI	TGATCA	2 cut(s)	64, 634
Fnu4HI	GCNGC	2 cut(s)	350, 1014
FriOI	GRGCYC	1 cut(s)	726
Fsp4HI	GCNGC	2 cut(s)	350, 1014
FspBI	CTAG	3 cut(s)	540, 644, 1049
GluI	GCNGC	2 cut(s)	350, 1014
GsaI	CCCAGC	1 cut(s)	1158
GsuI	CTGGAG	1 cut(s)	224
HaeIII	GGCC	3 cut(s)	40, 412, 926
HapII	CCGG	2 cut(s)	282, 1031
HgaI	GACGC	1 cut(s)	998
Hin1II	CATG	5 cut(s)	91, 451, 654, 1007, 1022
HinfI	GANTC	7 cut(s)	276, 502, 614, 914, 1034, 1110, 1182
HpaII	CCGG	2 cut(s)	282, 1031
HphI	GGTGA	2 cut(s)	194, 325
Hpy166II	GTNNAC	2 cut(s)	466, 988
Hpy188I	TCNGA	4 cut(s)	96, 297, 1077, 1181
Hpy188III	TCNNGA	6 cut(s)	215, 552, 611, 1107, 1139, 1186
Hpy8I	GTNNAC	2 cut(s)	466, 988
HpyAV	CCTTC	5 cut(s)	749, 853, 1136, 1144, 1183
HpyCH4III	ACNGT	2 cut(s)	373, 939
HpyCH4IV	ACGT	1 cut(s)	784
HpyCH4V	TGCA	5 cut(s)	91, 567, 688, 770, 1013
HpyF10VI	GCNNNNNNNGC	2 cut(s)	149, 1019
HpyF3I	CTNAG	3 cut(s)	272, 725, 954
HpySE526I	ACGT	1 cut(s)	784
Hsp92II	CATG	5 cut(s)	91, 451, 654, 1007, 1022
Ksp22I	TGATCA	2 cut(s)	64, 634
Kzo9I	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
LmnI	GCTCC	2 cut(s)	658, 965
Lsp1109I	GCAGC	1 cut(s)	1025
LweI	GCATC	3 cut(s)	100, 1001, 1100
MaeI	CTAG	3 cut(s)	540, 644, 1049
MaeII	ACGT	1 cut(s)	784
MaeIII	GTNAC	5 cut(s)	154, 367, 443, 830, 997
MalI	GATC	7 cut(s)	66, 104, 294, 342, 516, 629, 636
MbiI	CCGCTC	1 cut(s)	742
MboI	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
MflI	RGATCY	1 cut(s)	514
MhlI	GDGCHC	2 cut(s)	726, 1048
MluCI	AATT	5 cut(s)	4, 186, 329, 472, 903
MlyI	GAGTC	4 cut(s)	285, 623, 908, 1119
MmeI	TCCRAC	1 cut(s)	1061
Mph1103I	ATGCAT	1 cut(s)	93
MroXI	GAANNNNTTC	3 cut(s)	501, 873, 1061
MseI	TTAA	3 cut(s)	60, 168, 789
MslI	CAYNNNNRTG	1 cut(s)	1004
MspCI	CTTAAG	1 cut(s)	788
MspI	CCGG	2 cut(s)	282, 1031
MspR9I	CCNGG	1 cut(s)	302
MvaI	CCWGG	1 cut(s)	302
MvnI	CGCG	1 cut(s)	553
MwoI	GCNNNNNNNGC	2 cut(s)	149, 1019
NdeII	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
NlaIII	CATG	5 cut(s)	91, 451, 654, 1007, 1022
NlaIV	GGNNCC	2 cut(s)	516, 883
NmuCI	GTSAC	5 cut(s)	154, 367, 443, 830, 997
NruI	TCGCGA	1 cut(s)	553
NsiI	ATGCAT	1 cut(s)	93
NspI	RCATGY	3 cut(s)	91, 451, 1007
OliI	CACNNNNGTG	1 cut(s)	1004
PaeI	GCATGC	1 cut(s)	91
PciI	ACATGT	2 cut(s)	447, 1003
PcsI	WCGNNNNNNNCGW	1 cut(s)	213
PdmI	GAANNNNTTC	3 cut(s)	501, 873, 1061
PfeI	GAWTC	3 cut(s)	502, 1034, 1182
PkrI	GCNGC	2 cut(s)	351, 1015
PleI	GAGTC	4 cut(s)	284, 622, 908, 1118
PpsI	GAGTC	4 cut(s)	284, 622, 908, 1118
PscI	ACATGT	2 cut(s)	447, 1003
PshBI	ATTAAT	1 cut(s)	60
Psp124BI	GAGCTC	1 cut(s)	726
Psp6I	CCWGG	1 cut(s)	300
PspFI	CCCAGC	1 cut(s)	1154
PspGI	CCWGG	1 cut(s)	300
PspN4I	GGNNCC	2 cut(s)	516, 883
PspPI	GGNCC	4 cut(s)	27, 39, 411, 882
PsuI	RGATCY	1 cut(s)	514
RruI	TCGCGA	1 cut(s)	553
RsaI	GTAC	3 cut(s)	249, 467, 530
RsaNI	GTAC	3 cut(s)	248, 466, 529
RseI	CAYNNNNRTG	1 cut(s)	1004
SacI	GAGCTC	1 cut(s)	726
SaqAI	TTAA	3 cut(s)	60, 168, 789
SatI	GCNGC	2 cut(s)	350, 1014
Sau3AI	GATC	7 cut(s)	64, 102, 292, 340, 514, 627, 634
Sau96I	GGNCC	4 cut(s)	27, 39, 411, 882
ScaI	AGTACT	1 cut(s)	530
SchI	GAGTC	4 cut(s)	285, 623, 908, 1119
ScrFI	CCNGG	1 cut(s)	302
SduI	GDGCHC	2 cut(s)	726, 1048
SfaNI	GCATC	3 cut(s)	100, 1001, 1100
SfcI	CTRYAG	1 cut(s)	935
SinI	GGWCC	2 cut(s)	27, 882
SmiMI	CAYNNNNRTG	1 cut(s)	1004
SmlI	CTYRAG	6 cut(s)	401, 431, 609, 788, 1105, 1119
SmoI	CTYRAG	6 cut(s)	401, 431, 609, 788, 1105, 1119
SphI	GCATGC	1 cut(s)	91
Sse9I	AATT	5 cut(s)	4, 186, 329, 472, 903
SsiI	CCGC	5 cut(s)	229, 349, 493, 506, 742
SspMI	CTAG	3 cut(s)	540, 644, 1049
SstI	GAGCTC	1 cut(s)	726
StyD4I	CCNGG	1 cut(s)	300
StyI	CCWWGG	1 cut(s)	42
TaaI	ACNGT	2 cut(s)	373, 939
TaiI	ACGT	1 cut(s)	787
TaqI	TCGA	5 cut(s)	594, 843, 912, 1165, 1173
TaqII	GACCGA	1 cut(s)	985
TasI	AATT	5 cut(s)	4, 186, 329, 472, 903
TatI	WGTACW	2 cut(s)	465, 528
TauI	GCSGC	1 cut(s)	352
TfiI	GAWTC	3 cut(s)	502, 1034, 1182
Tru1I	TTAA	3 cut(s)	60, 168, 789
Tru9I	TTAA	3 cut(s)	60, 168, 789
TscAI	CASTG	1 cut(s)	448
TseFI	GTSAC	5 cut(s)	154, 367, 443, 830, 997
TseI	GCWGC	1 cut(s)	1013
Tsp45I	GTSAC	5 cut(s)	154, 367, 443, 830, 997
TspDTI	ATGAA	4 cut(s)	17, 401, 473, 768
TspGWI	ACGGA	1 cut(s)	874
TspRI	CASTG	1 cut(s)	448
Vha464I	CTTAAG	1 cut(s)	788
VpaK11BI	GGWCC	2 cut(s)	27, 882
VspI	ATTAAT	1 cut(s)	60
XapI	RAATTY	2 cut(s)	4, 472
XceI	RCATGY	3 cut(s)	91, 451, 1007
XmnI	GAANNNNTTC	3 cut(s)	501, 873, 1061
XspI	CTAG	3 cut(s)	540, 644, 1049
ZrmI	AGTACT	1 cut(s)	530
Zsp2I	ATGCAT	1 cut(s)	93

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.