Rroxscaffold_4G00309170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0019488
Physical Location & Seq
Reverse (-)
30825091 .. 30842196
17106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309170.1

Sequence Viewer

Length: 702 bp
ATGGTGGTGATATCAAAGCCACTTGTTAGAGATAACTACGAAATTTGGAGATTACCCAAGTTCTTATTACACCGAACCAACAAAAGTTGTTGTGGAAGTAAACAAGAAAGTTGCCAGCATAAATCAGGATTGATCAAGTACTTCAATCTTGGAGTTGTCAGCATAAAAATAAACCAGTACACAAAATCAAGCAAGACTCATGGTTTGAATGATTTGGAAGGCCATAGTGGTGACGAGCGTTCCTGTGGGAAGGTGATTTCATTGATTTTCACCTTTGATGTCACTTTAATGACAGAGGTTTCCGCATATGTTAATGAAAGCAGCCCTTGCTTCGGTGGTTTGGAGCAAAACATTCGTGGTAAAGGTCAAGAAGTGATTCGTGGGCTTCCGGATCGGTGGGGCGTGGTTGGAGTATGCTTTGGGTTTGTGGCGGCGTGGCTCGGAGATCATACGTTTCCATGGCAAGGCAAGGATGGAAGAATGATGCTTTGGGGGATCGTGGTTATGGCGGAGCAAATCTGCTCTGTCCGGATCGGTCCGATCTGGATTGGATCTCTTCGAATTGATGCGGGAATGGGTTTGTTCGGCGGGCTGGAGTGGTTCCATGGTTCGGACGAGAGGTTGGCCGATGATGGGCGTGGTCCGTCGAGGATGATGGTTGGTGTTGGTCGTTGGACTTCGGGGTGGGCGGATCGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

25.9

Weight (kDa)

8.69

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019488)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028192
rosa_multiflora Rmu_sc0007358.1_g000010
rosa_roxburghii Rroxscaffold_4G00309170
rosa_samantha Rh4DG242700 Rh5DG571000 Rh6CG326800 Rh7AG391800 Rh7CG410700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 388, 528
AciI CCGC 6 cut(s) 303, 431, 509, 569, 588, 689
AclWI GGATC 5 cut(s) 399, 503, 539, 559, 699
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 1 cut(s) 42
AfaI GTAC 2 cut(s) 140, 179
AfiI CCNNNNNNNGG 4 cut(s) 332, 464, 610, 633
AgsI TTSAA 2 cut(s) 145, 208
AjuI GAANNNNNNNTTGG 2 cut(s) 472, 504
AlwI GGATC 5 cut(s) 399, 503, 539, 559, 699
Aor13HI TCCGGA 2 cut(s) 388, 528
AoxI GGCC 2 cut(s) 220, 624
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 1 cut(s) 42
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
Asp700I GAANNNNTTC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 536, 641
AsuHPI GGTGA 4 cut(s) 19, 242, 262, 265
AsuII TTCGAA 1 cut(s) 559
AvaII GGWCC 2 cut(s) 536, 641
BbvI GCAGC 1 cut(s) 333
BccI CCATC 3 cut(s) 467, 626, 649
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BclI TGATCA 1 cut(s) 132
BisI GCNGC 2 cut(s) 322, 432
BlsI GCNGC 2 cut(s) 323, 433
BmcAI AGTACT 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 536, 641
BmgT120I GGNCC 2 cut(s) 536, 641
BmiI GGNNCC 1 cut(s) 602
BmsI GCATC 2 cut(s) 474, 556
BpmI CTGGAG 1 cut(s) 614
Bpu14I TTCGAA 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 458, 604
BsaWI WCCGGW 2 cut(s) 388, 528
Bsc4I CCNNNNNNNGG 4 cut(s) 332, 464, 610, 633
Bse1I ACTGG 1 cut(s) 175
BseAI TCCGGA 2 cut(s) 388, 528
BseCI ATCGAT 1 cut(s) 694
BseDI CCNNGG 2 cut(s) 458, 604
BseGI GGATG 2 cut(s) 478, 657
BseLI CCNNNNNNNGG 4 cut(s) 332, 464, 610, 633
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 333
BshFI GGCC 2 cut(s) 222, 626
BshVI ATCGAT 1 cut(s) 694
BsiSI CCGG 2 cut(s) 389, 529
BslI CCNNNNNNNGG 4 cut(s) 332, 464, 610, 633
BsnI GGCC 2 cut(s) 222, 626
Bsp119I TTCGAA 1 cut(s) 559
Bsp13I TCCGGA 2 cut(s) 388, 528
Bsp143I GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
Bsp19I CCATGG 2 cut(s) 458, 604
BspACI CCGC 6 cut(s) 303, 431, 509, 569, 588, 689
BspANI GGCC 2 cut(s) 222, 626
BspDI ATCGAT 1 cut(s) 694
BspEI TCCGGA 2 cut(s) 388, 528
BspLI GGNNCC 1 cut(s) 602
BspPI GGATC 5 cut(s) 399, 503, 539, 559, 699
BspT104I TTCGAA 1 cut(s) 559
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 458, 604
BssMI GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
BssT1I CCWWGG 2 cut(s) 458, 604
Bst6I CTCTTC 1 cut(s) 561
BstAPI GCANNNNNTGC 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 116, 590
BstDSI CCRYGG 2 cut(s) 458, 604
BstF5I GGATG 2 cut(s) 478, 657
BstKTI GATC 9 cut(s) 135, 394, 448, 498, 534, 543, 554, 694, 698
BstMBI GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
Bsu15I ATCGAT 1 cut(s) 694
BsuRI GGCC 2 cut(s) 222, 626
BsuTUI ATCGAT 1 cut(s) 694
BtgI CCRYGG 2 cut(s) 458, 604
BtsCI GGATG 2 cut(s) 478, 657
Cac8I GCNNGC 2 cut(s) 116, 590
Cfr13I GGNCC 2 cut(s) 536, 641
ClaI ATCGAT 1 cut(s) 694
CpoI CGGWCCG 1 cut(s) 536
Csp6I GTAC 2 cut(s) 139, 178
CspI CGGWCCG 1 cut(s) 536
CviAII CATG 3 cut(s) 200, 459, 605
CviJI RGCY 7 cut(s) 19, 222, 324, 385, 439, 592, 626
CviKI_1 RGCY 7 cut(s) 19, 222, 324, 385, 439, 592, 626
CviQI GTAC 2 cut(s) 139, 178
DpnI GATC 9 cut(s) 134, 393, 447, 497, 533, 542, 553, 693, 697
DpnII GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
EciI GGCGGA 1 cut(s) 524
Eco130I CCWWGG 2 cut(s) 458, 604
Eco32I GATATC 1 cut(s) 12
Eco47I GGWCC 2 cut(s) 536, 641
EcoRV GATATC 1 cut(s) 12
EcoT14I CCWWGG 2 cut(s) 458, 604
ErhI CCWWGG 2 cut(s) 458, 604
FaeI CATG 3 cut(s) 203, 462, 608
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FatI CATG 3 cut(s) 199, 458, 604
FauI CCCGC 2 cut(s) 562, 581
FauNDI CATATG 1 cut(s) 307
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 322, 432
FokI GGATG 2 cut(s) 485, 664
Fsp4HI GCNGC 2 cut(s) 322, 432
GluI GCNGC 2 cut(s) 322, 432
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 2 cut(s) 222, 626
HapII CCGG 2 cut(s) 389, 529
Hin1II CATG 3 cut(s) 203, 462, 608
HinfI GANTC 2 cut(s) 196, 376
HpaII CCGG 2 cut(s) 389, 529
HphI GGTGA 4 cut(s) 19, 242, 262, 265
Hpy166II GTNNAC 2 cut(s) 101, 180
Hpy188I TCNGA 3 cut(s) 443, 540, 613
Hpy188III TCNNGA 6 cut(s) 126, 368, 389, 529, 544, 699
Hpy8I GTNNAC 2 cut(s) 101, 180
Hpy99I CGWCG 1 cut(s) 649
HpyAV CCTTC 2 cut(s) 212, 244
HpyCH4IV ACGT 1 cut(s) 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 3 cut(s) 203, 462, 608
Kpn2I TCCGGA 2 cut(s) 388, 528
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
LmnI GCTCC 2 cut(s) 343, 511
LpnPI CCDG 8 cut(s) 111, 128, 188, 256, 402, 529, 542, 578
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 474, 556
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 2 cut(s) 230, 280
MalI GATC 9 cut(s) 134, 393, 447, 497, 533, 542, 553, 693, 697
MboI GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
MboII GAAGA 2 cut(s) 489, 548
MflI RGATCY 1 cut(s) 551
MluCI AATT 2 cut(s) 42, 561
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 2 cut(s) 388, 653
MnlI CCTC 3 cut(s) 289, 612, 642
MroI TCCGGA 2 cut(s) 388, 528
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 2 cut(s) 287, 312
MslI CAYNNNNRTG 2 cut(s) 228, 287
MspI CCGG 2 cut(s) 389, 529
MwoI GCNNNNNNNGC 1 cut(s) 327
NcoI CCATGG 2 cut(s) 458, 604
NdeI CATATG 1 cut(s) 307
NdeII GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
NlaIII CATG 3 cut(s) 203, 462, 608
NlaIV GGNNCC 1 cut(s) 602
NmuCI GTSAC 2 cut(s) 230, 280
NspV TTCGAA 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 2 cut(s) 323, 433
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 602
PspPI GGNCC 2 cut(s) 536, 641
PsuI RGATCY 1 cut(s) 551
RsaI GTAC 2 cut(s) 140, 179
RsaNI GTAC 2 cut(s) 139, 178
RseI CAYNNNNRTG 2 cut(s) 228, 287
Rsr2I CGGWCCG 1 cut(s) 536
RsrII CGGWCCG 1 cut(s) 536
SaqAI TTAA 2 cut(s) 287, 312
SatI GCNGC 2 cut(s) 322, 432
Sau3AI GATC 9 cut(s) 132, 391, 445, 495, 531, 540, 551, 691, 695
Sau96I GGNCC 2 cut(s) 536, 641
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 1 cut(s) 190
SetI ASST 6 cut(s) 255, 275, 300, 367, 455, 623
SfaNI GCATC 2 cut(s) 474, 556
SfuI TTCGAA 1 cut(s) 559
SinI GGWCC 2 cut(s) 536, 641
SmiMI CAYNNNNRTG 2 cut(s) 228, 287
Sse9I AATT 2 cut(s) 42, 561
SsiI CCGC 6 cut(s) 303, 431, 509, 569, 588, 689
StyI CCWWGG 2 cut(s) 458, 604
TaiI ACGT 1 cut(s) 455
TaqI TCGA 3 cut(s) 559, 647, 694
TaqII GACCGA 1 cut(s) 524
TasI AATT 2 cut(s) 42, 561
TatI WGTACW 2 cut(s) 138, 177
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 287, 312
Tru9I TTAA 2 cut(s) 287, 312
TseFI GTSAC 2 cut(s) 230, 280
TseI GCWGC 1 cut(s) 321
Tsp45I GTSAC 2 cut(s) 230, 280
TspDTI ATGAA 2 cut(s) 249, 330
TspGWI ACGGA 1 cut(s) 633
VpaK11BI GGWCC 2 cut(s) 536, 641
XapI RAATTY 1 cut(s) 42
XmnI GAANNNNTTC 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.