Rroxscaffold_4G00317220

Geranylgeranyl diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016913
Physical Location & Seq
Reverse (-)
42349764 .. 42350726
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317220.1

Sequence Viewer

Length: 963 bp
ATGCTCCGCCGCGAGGTCCTCGACTCCTTCCTCCGCGACCGCGCCCAGTCCCGCGGCGCCTCCCTCATCTCCGGCCTCGTCACCCGCCTCGAGATCCCCGCCTCCTCCTCCCCCGACGCGCCCTACGTCGTCCACTACACCAAGGACCACGCGCGCCACGCGCTCGCCGTCGACGTCGTCGTCGGCGCGGATGGCGCCAACAGCCGCGTCGCCAAGTCGATCAGAGCCGGAAGCTACGCCTGCGCCATCGCTTTCCAGGAGAGGATCAAACTCCCAGACGACAAAATGGCCTACTACGAAGACCTCGCCGAAATGTACGTCGGAAACGACGTGTCGCCCGACTTTTACGCCTGGGTCTTCCCCAAATGCGACCACGTGGCAGTGGGCACCGGCACTGTGTGCTCCAAGCAGGACATCAAGGTCTTCCAAAGGGCCATCAGGGCCAGAGCCAATGCCAAGATCAGCGGTGGCAGAGTCATCAAGGTGGAGGCCCACCCGATACCCGAGCACCCCCGCCCCACGCGCGTGAGGGGGCGCGTGGCGCTCATTGGCGACGCTGCCGGGTACGTGACGAAATGTTCCGGCGAGGGGATTTACTTTGCGGCGAAATCGGGGAGAATGTGCGGCGAGGCAATAATAAAGGCGTCGGAGGGAGGGGAGAGGATGATAAATGAGGAGGATTTGAAGAGGGAGTATTTGAAAGAGTGGGATGCTAAGTACATTACGACGTTTAGGTTCTTGGACTTGTTGCAGAAGGTGTTTTATGGTAGTGATGCGGCTAGAGAAGCTTTGGTGGAGCTGTGTGGGGATGAGTATGTGCAGCGCATGACGTTCGAGAGTTACTTGTACAAGAAGTTAGCTGATGGGAATAGGTGGGATGATGCTAAGATGGTTTTGAATACCGTTGGGAGTCTGATGAGGTGTAACATTTTGGGGAGGAAAATGGAGGCTTTGAAGATTTGA

Protein Analysis

320

Amino Acids

35.66

Weight (kDa)

8.82

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGR_cat PF22578 98 - 140 8.8e-07 Geranylgeranyl reductase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016913)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06120
malus_domestica MD00G1178300.v1.1
prunus_persica Prupe.6G153000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0333811
rosa_laevigata RLG00000029529
rosa_multiflora Rmu_sc0000026.1_g000018 Rmu_sc0007177.1_g000004
rosa_roxburghii Rroxscaffold_4G00317220
rosa_rugosa Rorug01G0112000
rosa_samantha Rh1AG134400 Rh1BG102900 Rh1CG128000 Rh1DG139900
rosa_wichuraiana Rw1G011210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 179, 419
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 3 cut(s) 56, 194, 386
AccI GTMKAC 1 cut(s) 171
AclWI GGATC 2 cut(s) 88, 272
AcvI CACGTG 1 cut(s) 376
AcyI GRCGYC 4 cut(s) 57, 174, 195, 644
AdeI CACNNNGTG 1 cut(s) 399
AfaI GTAC 4 cut(s) 317, 566, 719, 848
AfiI CCNNNNNNNGG 2 cut(s) 13, 588
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 4 cut(s) 685, 700, 898, 955
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 2 cut(s) 255, 350
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 4 cut(s) 234, 788, 799, 860
AluI AGCT 4 cut(s) 234, 788, 799, 860
Alw21I GWGCWC 2 cut(s) 404, 510
AlwI GGATC 2 cut(s) 88, 272
Ama87I CYCGRG 2 cut(s) 89, 503
AoxI GGCC 5 cut(s) 73, 288, 432, 441, 489
ApeKI GCWGC 2 cut(s) 557, 820
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspS9I GGNCC 5 cut(s) 16, 145, 432, 441, 490
AsuC2I CCSGG 1 cut(s) 562
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 2 cut(s) 89, 503
AvaII GGWCC 2 cut(s) 16, 145
BaeGI GKGCMC 1 cut(s) 389
BanI GGYRCC 3 cut(s) 56, 194, 386
BbrPI CACGTG 1 cut(s) 376
BbsI GAAGAC 3 cut(s) 306, 349, 415
Bbv12I GWGCWC 2 cut(s) 404, 510
BbvI GCAGC 2 cut(s) 544, 832
BccI CCATC 5 cut(s) 185, 254, 443, 857, 883
BceAI ACGGC 1 cut(s) 152
BcgI CGANNNNNNTGC 2 cut(s) 814, 848
BciT130I CCWGG 2 cut(s) 257, 352
BcnI CCSGG 1 cut(s) 562
BfaI CTAG 1 cut(s) 780
BfoI RGCGCY 3 cut(s) 60, 198, 545
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 8 cut(s) 10, 55, 205, 558, 603, 625, 777, 821
BlsI GCNGC 8 cut(s) 11, 56, 206, 559, 604, 626, 778, 822
Bme1390I CCNGG 3 cut(s) 257, 352, 562
Bme18I GGWCC 2 cut(s) 16, 145
BmeT110I CYCGRG 2 cut(s) 89, 503
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 5 cut(s) 16, 145, 432, 441, 490
BmiI GGNNCC 3 cut(s) 58, 196, 388
BmrFI CCNGG 3 cut(s) 257, 352, 562
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 3 cut(s) 700, 763, 871
BmuI ACTGGG 1 cut(s) 40
BpiI GAAGAC 3 cut(s) 306, 349, 415
BpuMI CCSGG 1 cut(s) 562
BsaAI YACGTR 2 cut(s) 376, 568
BsaHI GRCGYC 4 cut(s) 57, 174, 195, 644
BsaJI CCNNGG 3 cut(s) 52, 141, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 588
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 2 cut(s) 257, 352
BseDI CCNNGG 3 cut(s) 52, 141, 351
BseGI GGATG 5 cut(s) 196, 669, 715, 814, 883
BseLI CCNNNNNNNGG 2 cut(s) 13, 588
BseNI ACTGG 1 cut(s) 46
BsePI GCGCGC 1 cut(s) 152
BseRI GAGGAG 3 cut(s) 94, 97, 689
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 2 cut(s) 544, 832
BsgI GTGCAG 1 cut(s) 839
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 5 cut(s) 75, 290, 434, 443, 491
BshNI GGYRCC 3 cut(s) 56, 194, 386
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 2 cut(s) 404, 510
BsiHKCI CYCGRG 2 cut(s) 89, 503
BsiSI CCGG 5 cut(s) 72, 228, 390, 561, 582
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 2 cut(s) 13, 588
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 5 cut(s) 75, 290, 434, 443, 491
BsoBI CYCGRG 2 cut(s) 89, 503
Bsp1286I GDGCHC 3 cut(s) 389, 404, 510
Bsp1407I TGTACA 1 cut(s) 846
Bsp143I GATC 4 cut(s) 93, 219, 264, 459
BspANI GGCC 5 cut(s) 75, 290, 434, 443, 491
BspLI GGNNCC 3 cut(s) 58, 196, 388
BspPI GGATC 2 cut(s) 88, 272
BspT107I GGYRCC 3 cut(s) 56, 194, 386
BsrFI RCCGGY 1 cut(s) 389
BsrGI TGTACA 1 cut(s) 846
BsrI ACTGG 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 52, 141, 351
BssHII GCGCGC 1 cut(s) 152
BssMI GATC 4 cut(s) 93, 219, 264, 459
BssNI GRCGYC 4 cut(s) 57, 174, 195, 644
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 257, 352
Bst4CI ACNGT 2 cut(s) 397, 904
Bst6I CTCTTC 1 cut(s) 680
BstACI GRCGYC 4 cut(s) 57, 174, 195, 644
BstAPI GCANNNNNTGC 1 cut(s) 399
BstAUI TGTACA 1 cut(s) 846
BstBAI YACGTR 2 cut(s) 376, 568
BstC8I GCNNGC 3 cut(s) 154, 165, 241
BstDEI CTNAG 2 cut(s) 714, 885
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 5 cut(s) 196, 669, 715, 814, 883
BstH2I RGCGCY 3 cut(s) 60, 198, 545
BstKTI GATC 4 cut(s) 96, 222, 267, 462
BstMBI GATC 4 cut(s) 93, 219, 264, 459
BstMCI CGRYCG 1 cut(s) 40
BstNI CCWGG 2 cut(s) 257, 352
BstSCI CCNGG 3 cut(s) 255, 350, 560
BstSLI GKGCMC 1 cut(s) 389
BstV1I GCAGC 2 cut(s) 544, 832
BstV2I GAAGAC 3 cut(s) 306, 349, 415
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
BsuRI GGCC 5 cut(s) 75, 290, 434, 443, 491
BtgI CCRYGG 1 cut(s) 52
BtgZI GCGATG 1 cut(s) 232
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 5 cut(s) 196, 669, 715, 814, 883
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 387, 393
Cac8I GCNNGC 3 cut(s) 154, 165, 241
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 5 cut(s) 16, 145, 432, 441, 490
Cfr42I CCGCGG 1 cut(s) 55
CseI GACGC 4 cut(s) 125, 196, 563, 633
Csp6I GTAC 4 cut(s) 316, 565, 718, 847
CviAII CATG 1 cut(s) 826
CviQI GTAC 4 cut(s) 316, 565, 718, 847
DdeI CTNAG 2 cut(s) 714, 885
DinI GGCGCC 2 cut(s) 58, 196
DpnI GATC 4 cut(s) 95, 221, 266, 461
DpnII GATC 4 cut(s) 93, 219, 264, 459
DraIII CACNNNGTG 1 cut(s) 399
DrdI GACNNNNNNGTC 2 cut(s) 179, 419
DseDI GACNNNNNNGTC 2 cut(s) 179, 419
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 16, 145
Eco72I CACGTG 1 cut(s) 376
Eco88I CYCGRG 2 cut(s) 89, 503
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 255, 350
EcoT14I CCWWGG 1 cut(s) 141
EgeI GGCGCC 2 cut(s) 58, 196
EheI GGCGCC 2 cut(s) 58, 196
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 829
FaiI YATR 3 cut(s) 765, 816, 827
FaqI GGGAC 1 cut(s) 34
FatI CATG 1 cut(s) 825
FauI CCCGC 4 cut(s) 59, 92, 106, 521
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 8 cut(s) 10, 55, 205, 558, 603, 625, 777, 821
FokI GGATG 5 cut(s) 203, 676, 722, 821, 890
Fsp4HI GCNGC 8 cut(s) 10, 55, 205, 558, 603, 625, 777, 821
FspBI CTAG 1 cut(s) 780
GluI GCNGC 8 cut(s) 10, 55, 205, 558, 603, 625, 777, 821
HaeII RGCGCY 3 cut(s) 60, 198, 545
HaeIII GGCC 5 cut(s) 75, 290, 434, 443, 491
HapII CCGG 5 cut(s) 72, 228, 390, 561, 582
HgaI GACGC 4 cut(s) 125, 196, 563, 633
Hin1I GRCGYC 4 cut(s) 57, 174, 195, 644
Hin1II CATG 1 cut(s) 829
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 3 cut(s) 23, 474, 910
HpaII CCGG 5 cut(s) 72, 228, 390, 561, 582
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 133, 172
Hpy188I TCNGA 4 cut(s) 224, 323, 649, 915
Hpy188III TCNNGA 2 cut(s) 91, 835
Hpy8I GTNNAC 2 cut(s) 133, 172
HpyAV CCTTC 2 cut(s) 37, 748
HpyCH4III ACNGT 2 cut(s) 397, 904
HpyCH4IV ACGT 8 cut(s) 126, 174, 318, 330, 375, 567, 728, 830
HpyCH4V TGCA 2 cut(s) 751, 820
HpyF3I CTNAG 2 cut(s) 714, 885
HpySE526I ACGT 8 cut(s) 126, 174, 318, 330, 375, 567, 728, 830
Hsp92I GRCGYC 4 cut(s) 57, 174, 195, 644
Hsp92II CATG 1 cut(s) 829
KasI GGCGCC 2 cut(s) 56, 194
KspI CCGCGG 1 cut(s) 55
Kzo9I GATC 4 cut(s) 93, 219, 264, 459
LmnI GCTCC 3 cut(s) 9, 407, 796
Lsp1109I GCAGC 2 cut(s) 544, 832
LweI GCATC 3 cut(s) 700, 763, 871
MaeI CTAG 1 cut(s) 780
MaeII ACGT 8 cut(s) 126, 174, 318, 330, 375, 567, 728, 830
MaeIII GTNAC 4 cut(s) 79, 568, 839, 923
MalI GATC 4 cut(s) 95, 221, 266, 461
MboI GATC 4 cut(s) 93, 219, 264, 459
MboII GAAGA 4 cut(s) 311, 349, 415, 697
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 3 cut(s) 389, 404, 510
Mly113I GGCGCC 2 cut(s) 57, 195
MlyI GAGTC 3 cut(s) 17, 483, 919
MmeI TCCRAC 2 cut(s) 301, 627
MslI CAYNNNNRTG 2 cut(s) 482, 524
MspA1I CMGCKG 2 cut(s) 54, 465
MspI CCGG 5 cut(s) 72, 228, 390, 561, 582
MspR9I CCNGG 3 cut(s) 257, 352, 562
MvaI CCWGG 2 cut(s) 257, 352
NarI GGCGCC 2 cut(s) 57, 195
NciI CCSGG 1 cut(s) 562
NdeII GATC 4 cut(s) 93, 219, 264, 459
NlaIII CATG 1 cut(s) 829
NlaIV GGNNCC 3 cut(s) 58, 196, 388
NmuCI GTSAC 2 cut(s) 79, 568
OliI CACNNNNGTG 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 89
PauI GCGCGC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 7 cut(s) 123, 165, 171, 177, 324, 327, 336
PflFI GACNNNGTC 1 cut(s) 176
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 8 cut(s) 11, 56, 206, 559, 604, 626, 778, 822
PleI GAGTC 3 cut(s) 17, 482, 918
PluTI GGCGCC 2 cut(s) 60, 198
PmaCI CACGTG 1 cut(s) 376
PmlI CACGTG 1 cut(s) 376
PpsI GAGTC 3 cut(s) 17, 482, 918
Ppu21I YACGTR 2 cut(s) 376, 568
PpuMI RGGWCCY 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 255, 350
PspCI CACGTG 1 cut(s) 376
PspGI CCWGG 2 cut(s) 255, 350
PspN4I GGNNCC 3 cut(s) 58, 196, 388
PspPI GGNCC 5 cut(s) 16, 145, 432, 441, 490
PspPPI RGGWCCY 1 cut(s) 16
PsuI RGATCY 1 cut(s) 93
PsyI GACNNNGTC 1 cut(s) 176
PteI GCGCGC 1 cut(s) 152
RsaI GTAC 4 cut(s) 317, 566, 719, 848
RsaNI GTAC 4 cut(s) 316, 565, 718, 847
RseI CAYNNNNRTG 2 cut(s) 482, 524
SacII CCGCGG 1 cut(s) 55
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 8 cut(s) 10, 55, 205, 558, 603, 625, 777, 821
Sau3AI GATC 4 cut(s) 93, 219, 264, 459
Sau96I GGNCC 5 cut(s) 16, 145, 432, 441, 490
SchI GAGTC 3 cut(s) 17, 483, 919
ScrFI CCNGG 3 cut(s) 257, 352, 562
SduI GDGCHC 3 cut(s) 389, 404, 510
SfaNI GCATC 3 cut(s) 700, 763, 871
SfiI GGCCNNNNNGGCC 1 cut(s) 440
SfoI GGCGCC 2 cut(s) 58, 196
Sfr274I CTCGAG 1 cut(s) 89
Sfr303I CCGCGG 1 cut(s) 55
SgrBI CCGCGG 1 cut(s) 55
SgrDI CGTCGACG 1 cut(s) 170
SinI GGWCC 2 cut(s) 16, 145
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 2 cut(s) 482, 524
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SspDI GGCGCC 2 cut(s) 56, 194
SspMI CTAG 1 cut(s) 780
StyD4I CCNGG 3 cut(s) 255, 350, 560
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 397, 904
TaiI ACGT 8 cut(s) 129, 177, 321, 333, 378, 570, 731, 833
TaqI TCGA 5 cut(s) 21, 90, 171, 218, 834
TatI WGTACW 2 cut(s) 717, 846
TauI GCSGC 6 cut(s) 12, 57, 207, 605, 627, 779
TscAI CASTG 2 cut(s) 387, 400
TseFI GTSAC 2 cut(s) 79, 568
TseI GCWGC 2 cut(s) 557, 820
Tsp45I GTSAC 2 cut(s) 79, 568
TspRI CASTG 2 cut(s) 387, 400
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 16, 145
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 780
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.