Rroxscaffold_5G00347130

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015227
Physical Location & Seq
Forward (+)
18319918 .. 18323814
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347130.1

Sequence Viewer

Length: 2100 bp
ATGAGGGTATTCCATTCACTTGTGAATGCTTTGCGCAAGGTCTCGATAACCCACTGCAGGGAAATCAAGTTAGGAGCCTTGGCAGACGTTTACTGTGCGAACAATATCCTAAACGGGTACGCGAGATGTCAACACCTGTGCCTTGCCCACCAGGTGTTCGACGAAATGCCCCACAGAGACACCGTGTCTTGGAACACGATTATTGCTGGAAATGTGAACTGCGGGGAGTTCGAGGCCGCGTGGGAGATACTAAGAAGCATGAGAAGATGTGGGTTTGATTTTGATGGGTATACATTTGGAAGCATAGTTAAGGGTGTCGCTTGTGCTCTTCGATACGATCTTGGGCAGCAAGTGCATTCCTTGGTTCTTAAGGTAGGTTATGCCAGTAATGTTTATTCTGGAAGTGCTCTTTTGGACATGTACGCGAAATGTGGGAGAGTCGAGGATGCATATGTGGTCTTTGAATCATTGCCGCAACGCAACTCTGTTTCGTGGAATGCACTCATTGCTGGTTACGTGCAAGCAGGTGATCGCAGCATGACGTTTTGGCTATTACATTGTATGGAGCAGGAGGGTCTGCAGCTGGAAGATGGCACAATTGCTCCACTTTTGACATTGCTTGATGATGCTGAGTTTTATGAGTCCTTGGTACAGATCCATTGTAAAATTATTAAACATGGGCTGGGATGTGAAAGTACGGTCTGCAATGCTACAATCACTTCCTATTCAGAATGTGGGTCAATAGAAGAGGCCGAAAGAGTGTTTGATGGTTCTTTTGGTAAAAGAGATTTGGTGACATGGAATTCCATGCTAGCAGCGTACTTGGTTCACGGAAAAGAACAACTTGCTTGCAAACTCTTCTTGGACATGCAAAGGCTTGGGTTTGAACCAGATATCTACAGTTATACTAGCATCATCACTGCCTACTCTGAAGAGGTACACAAGAATCATGGGAGATCCTTTCATGGACTGGTCATAAAAAGGGGACTAGAAAAATCTGGACCAATCTCTAATGCGTTAATTGCTATGTATCTCAAGTCAAGTAACAATTCCTTGGAGGAAGCACTACATGTCTTTGAATCCTTGGAGTTTAAGGACCGTGTTTCTTGGAACTCCATTTTGACTGGGTTTTCACAGAATGGGTGGAATGAAGATGCCTTGAGATTATTTGGGCATATGAGATATGGAGAGGTGGAGGTTGATCATTATGCCTTCTCAGCTGTACTTCGGTCTTGCTCAGATTTAGGAACCCTCAACCTGGGTCAACAGGTTCATGCTTTGACACTTAGATCAGGGCTTGAATCCAATGAGTTTGTAGCTAGTTCTTTGATCTCTATGTATTCCAAGTGCGGGATCATTGACGATGCTAGAAAATCGTTTGAAGAGACTCCTAAAGATAGCGCAATCACTTGGAATTCAATTATGTTTGGATATGCACAAAATGGGCAGGGATATGTTGCACTGGACCTCTTCTCCCAAATGAAGAAGGAAAAGGTGAAACTTGATCACATAACATTTGTGGCAATTCTAACTGCTTGTAGCCACGTGGGTTTGGTTGAACAAGGATGCAAGTTTCTAAATTCTATGAAATCTGAATACACAATTGCACCACGAATGGAGCATTATGCTTGTGCAGTAGATCTATATGGGCGCGCTGGGCATCTAGATAAGGCAAAAGCCTTGATTGAAGAAATGCCATTTGAACCTGATGCAATGGTGTGGAAGACTTTACTGGGTGCCTGCAGGGCTTGTGGCAATATTGAATTGGCGAGTCAAGTAGCAGACTATCTGCTAGTGCTAGAGCCTGAAGAGCACTGCACTTATGTTCTTCTTTCTGACATGTATGGACATCTTAAAAGGTGGGATGCTAAAGCTAGTGTAAAGAAGCTTATGAGGGAAAGGGGTGTTAGGAAAGTCCCTGGTTGGAGTTGGATAGAACTCAACAATGAGGTGCATGCATTTAAAGCTGAAGATCATTCACACCCTCAGTGTGAAGAGATATATTTTGTATTGGGAATTCTGATGGAGGAAATCAAAAGGCTGGACATTGTTGCTAATCTAAAACAGTCTTTAGGTGAGGGAGATGACAGTGGGACTTGA

Protein Analysis

699

Amino Acids

78.38

Weight (kDa)

5.63

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 59 - 107 8.5e-10 PPR repeat family
PPR_3 PF13812 262 - 310 4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 263 - 309 1.3e-12 PPR repeat family
PPR_2 PF13041 366 - 413 3.2e-07 PPR repeat family
PPR_2 PF13041 468 - 514 2.6e-10 PPR repeat family
PPR PF01535 469 - 498 7.1e-06 PPR repeat
E_motif PF20431 586 - 646 2.4e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25970
fragaria_vesca FvH4_3g28520
malus_domestica MD15G1043900.v1.1
prunus_persica Prupe.1G397500_v2.0.a1
pyrus_communis pycom15g04090
rosa_chinensis RchiOBHm_Chr4g0402431
rosa_laevigata RLG00000009084
rosa_multiflora Rmu_co8477433.1_g000001 Rmu_ssc0000330.1_g000025
rosa_roxburghii Rroxscaffold_5G00347130
rosa_rugosa Rorug04G0035300
rosa_samantha Rh4AG112700 Rh4BG105200 Rh4CG119500 Rh4DG104500
rosa_wichuraiana Rw4G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 515
Acc16I TGCGCA 1 cut(s) 35
Acc36I ACCTGC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 1736
AccI GTMKAC 1 cut(s) 290
AccII CGCG 4 cut(s) 122, 239, 425, 1653
AciI CCGC 4 cut(s) 222, 237, 473, 1350
AclWI GGATC 3 cut(s) 649, 951, 1361
AcsI RAATTY 4 cut(s) 802, 1414, 1579, 2016
AcuI CTGAAG 3 cut(s) 951, 1827, 1989
AcvI CACGTG 1 cut(s) 1546
AdeI CACNNNGTG 1 cut(s) 154
AfaI GTAC 7 cut(s) 119, 422, 651, 697, 821, 939, 1224
AfiI CCNNNNNNNGG 5 cut(s) 57, 58, 189, 1258, 1350
AflII CTTAAG 1 cut(s) 368
AflIII ACRYGT 3 cut(s) 417, 1069, 1839
AhdI GACNNNNNGTC 1 cut(s) 184
AjnI CCWGG 3 cut(s) 150, 1257, 1918
AluBI AGCT 6 cut(s) 583, 1220, 1319, 1874, 1888, 1967
AluI AGCT 6 cut(s) 583, 1220, 1319, 1874, 1888, 1967
Alw21I GWGCWC 3 cut(s) 328, 409, 1815
Alw26I GTCTC 3 cut(s) 46, 171, 1379
AlwI GGATC 3 cut(s) 649, 951, 1361
AoxI GGCC 2 cut(s) 234, 750
ApeKI GCWGC 4 cut(s) 346, 534, 580, 815
ApoI RAATTY 4 cut(s) 802, 1414, 1579, 2016
AspLEI GCGC 4 cut(s) 36, 1403, 1653, 1655
AspS9I GGNCC 3 cut(s) 1001, 1096, 1465
AsuHPI GGTGA 4 cut(s) 539, 805, 1507, 2087
AsuNHI GCTAGC 1 cut(s) 811
AvaII GGWCC 3 cut(s) 1001, 1096, 1465
BanI GGYRCC 1 cut(s) 1736
BbrPI CACGTG 1 cut(s) 1546
BbsI GAAGAC 1 cut(s) 1730
Bbv12I GWGCWC 3 cut(s) 328, 409, 1815
BbvI GCAGC 4 cut(s) 358, 546, 592, 827
BccI CCATC 4 cut(s) 278, 584, 761, 2017
BciT130I CCWGG 3 cut(s) 152, 1259, 1920
BclI TGATCA 2 cut(s) 1201, 1504
BcoDI GTCTC 3 cut(s) 46, 171, 1379
BfaI CTAG 9 cut(s) 812, 909, 989, 1320, 1368, 1664, 1793, 1799, 1875
BfmI CTRYAG 4 cut(s) 55, 578, 898, 1741
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 1745
BglII AGATCT 1 cut(s) 1639
BisI GCNGC 6 cut(s) 237, 347, 473, 535, 581, 816
BlsI GCNGC 6 cut(s) 238, 348, 474, 536, 582, 817
Bme1390I CCNGG 3 cut(s) 152, 1259, 1920
Bme18I GGWCC 3 cut(s) 1001, 1096, 1465
BmeRI GACNNNNNGTC 1 cut(s) 184
BmgT120I GGNCC 3 cut(s) 1001, 1096, 1465
BmiI GGNNCC 3 cut(s) 76, 1249, 1738
BmrFI CCNGG 3 cut(s) 152, 1259, 1920
BmrI ACTGGG 2 cut(s) 1134, 1742
BmsI GCATC 9 cut(s) 436, 616, 921, 1144, 1354, 1556, 1669, 1699, 1855
BmtI GCTAGC 1 cut(s) 815
BmuI ACTGGG 2 cut(s) 1134, 1742
BpiI GAAGAC 1 cut(s) 1730
BpuEI CTTGAG 2 cut(s) 1019, 1180
BsaAI YACGTR 2 cut(s) 517, 1546
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 7 cut(s) 78, 360, 645, 1053, 1083, 1258, 1918
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 1457, 1487
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 58, 189, 1258, 1350
Bse1I ACTGG 5 cut(s) 384, 975, 1129, 1467, 1737
Bse3DI GCAATG 5 cut(s) 467, 504, 614, 712, 1719
BseBI CCWGG 3 cut(s) 152, 1259, 1920
BseDI CCNNGG 7 cut(s) 78, 360, 645, 1053, 1083, 1258, 1918
BseGI GGATG 4 cut(s) 451, 692, 1571, 1870
BseLI CCNNNNNNNGG 5 cut(s) 57, 58, 189, 1258, 1350
BseMI GCAATG 5 cut(s) 467, 504, 614, 712, 1719
BseMII CTCAG 4 cut(s) 621, 1230, 1251, 2000
BseNI ACTGG 5 cut(s) 384, 975, 1129, 1467, 1737
BsePI GCGCGC 1 cut(s) 1651
BseXI GCAGC 4 cut(s) 358, 546, 592, 827
BseYI CCCAGC 2 cut(s) 682, 1655
BsgI GTGCAG 2 cut(s) 1653, 1801
Bsh1236I CGCG 4 cut(s) 122, 239, 425, 1653
BshFI GGCC 2 cut(s) 236, 752
BshNI GGYRCC 1 cut(s) 1736
BsiHKAI GWGCWC 3 cut(s) 328, 409, 1815
BslFI GGGAC 2 cut(s) 999, 1901
BslI CCNNNNNNNGG 5 cut(s) 57, 58, 189, 1258, 1350
BsmAI GTCTC 3 cut(s) 46, 171, 1379
BsmFI GGGAC 2 cut(s) 999, 1901
BsmI GAATGC 3 cut(s) 31, 355, 502
BsnI GGCC 2 cut(s) 236, 752
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 3 cut(s) 328, 409, 1815
BspACI CCGC 4 cut(s) 222, 237, 473, 1350
BspANI GGCC 2 cut(s) 236, 752
BspCNI CTCAG 4 cut(s) 622, 1229, 1250, 1999
BspFNI CGCG 4 cut(s) 122, 239, 425, 1653
BspLI GGNNCC 3 cut(s) 76, 1249, 1738
BspMAI CTGCAG 3 cut(s) 59, 582, 1745
BspMI ACCTGC 1 cut(s) 515
BspOI GCTAGC 1 cut(s) 815
BspPI GGATC 3 cut(s) 649, 951, 1361
BspQI GCTCTTC 2 cut(s) 333, 1803
BspT107I GGYRCC 1 cut(s) 1736
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 5 cut(s) 467, 504, 614, 712, 1719
BsrI ACTGG 5 cut(s) 384, 975, 1129, 1467, 1737
BssECI CCNNGG 7 cut(s) 78, 360, 645, 1053, 1083, 1258, 1918
BssHII GCGCGC 1 cut(s) 1651
BssNAI GTATAC 1 cut(s) 291
BssT1I CCWWGG 5 cut(s) 78, 360, 645, 1053, 1083
Bst1107I GTATAC 1 cut(s) 291
Bst2UI CCWGG 3 cut(s) 152, 1259, 1920
Bst4CI ACNGT 7 cut(s) 95, 184, 700, 902, 1100, 2067, 2090
Bst6I CTCTTC 8 cut(s) 333, 741, 863, 927, 1377, 1475, 1803, 1989
BstAFI CTTAAG 1 cut(s) 368
BstAPI GCANNNNNTGC 2 cut(s) 352, 506
BstBAI YACGTR 2 cut(s) 517, 1546
BstC8I GCNNGC 6 cut(s) 522, 813, 850, 1653, 1741, 1956
BstDEI CTNAG 6 cut(s) 251, 630, 1216, 1237, 1286, 1986
BstF5I GGATG 4 cut(s) 451, 692, 1571, 1870
BstFNI CGCG 4 cut(s) 122, 239, 425, 1653
BstHHI GCGC 4 cut(s) 36, 1403, 1653, 1655
BstMAI GTCTC 3 cut(s) 46, 171, 1379
BstMWI GCNNNNNNNGC 8 cut(s) 352, 506, 1022, 1217, 1657, 1745, 1810, 1964
BstNI CCWGG 3 cut(s) 152, 1259, 1920
BstNSI RCATGY 5 cut(s) 421, 871, 1073, 1843, 1958
BstSCI CCNGG 3 cut(s) 150, 1257, 1918
BstSFI CTRYAG 4 cut(s) 55, 578, 898, 1741
BstUI CGCG 4 cut(s) 122, 239, 425, 1653
BstV1I GCAGC 4 cut(s) 358, 546, 592, 827
BstV2I GAAGAC 1 cut(s) 1730
BstX2I RGATCY 3 cut(s) 654, 956, 1639
BstYI RGATCY 3 cut(s) 654, 956, 1639
BstZ17I GTATAC 1 cut(s) 291
BsuRI GGCC 2 cut(s) 236, 752
BtsCI GGATG 4 cut(s) 451, 692, 1571, 1870
BtsI GCAGTG 3 cut(s) 52, 918, 1813
BtsIMutI CAGTG 6 cut(s) 52, 918, 1460, 1813, 1994, 2095
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 6 cut(s) 522, 813, 850, 1653, 1741, 1956
CfoI GCGC 4 cut(s) 36, 1403, 1653, 1655
Cfr13I GGNCC 3 cut(s) 1001, 1096, 1465
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 7 cut(s) 118, 421, 650, 696, 820, 938, 1223
CviQI GTAC 7 cut(s) 118, 421, 650, 696, 820, 938, 1223
DdeI CTNAG 6 cut(s) 251, 630, 1216, 1237, 1286, 1986
DraI TTTAAA 1 cut(s) 1963
DraIII CACNNNGTG 1 cut(s) 154
DriI GACNNNNNGTC 1 cut(s) 184
Eam1104I CTCTTC 8 cut(s) 333, 741, 863, 927, 1377, 1475, 1803, 1989
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 8 cut(s) 333, 741, 863, 927, 1377, 1475, 1803, 1989
Eco130I CCWWGG 5 cut(s) 78, 360, 645, 1053, 1083
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 895
Eco47I GGWCC 3 cut(s) 1001, 1096, 1465
Eco57I CTGAAG 3 cut(s) 951, 1827, 1989
Eco72I CACGTG 1 cut(s) 1546
EcoRI GAATTC 3 cut(s) 802, 1414, 2016
EcoRII CCWGG 3 cut(s) 150, 1257, 1918
EcoRV GATATC 1 cut(s) 895
EcoT14I CCWWGG 5 cut(s) 78, 360, 645, 1053, 1083
EcoT22I ATGCAT 2 cut(s) 451, 1960
ErhI CCWWGG 5 cut(s) 78, 360, 645, 1053, 1083
FalI AAGNNNNNCTT 2 cut(s) 828, 860
FaqI GGGAC 2 cut(s) 999, 1901
FauI CCCGC 2 cut(s) 215, 1343
FauNDI CATATG 2 cut(s) 451, 1176
FbaI TGATCA 2 cut(s) 1201, 1504
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 6 cut(s) 237, 347, 473, 535, 581, 816
FokI GGATG 4 cut(s) 458, 699, 1578, 1877
Fsp4HI GCNGC 6 cut(s) 237, 347, 473, 535, 581, 816
FspBI CTAG 9 cut(s) 812, 909, 989, 1320, 1368, 1664, 1793, 1799, 1875
FspI TGCGCA 1 cut(s) 35
GlaI GCGC 4 cut(s) 35, 1402, 1652, 1654
GluI GCNGC 6 cut(s) 237, 347, 473, 535, 581, 816
GsaI CCCAGC 2 cut(s) 686, 1659
HaeIII GGCC 2 cut(s) 236, 752
HhaI GCGC 4 cut(s) 36, 1403, 1653, 1655
Hin6I GCGC 4 cut(s) 34, 1401, 1651, 1653
HinP1I GCGC 4 cut(s) 34, 1401, 1651, 1653
HincII GTYRAC 2 cut(s) 131, 1265
HindII GTYRAC 2 cut(s) 131, 1265
HindIII AAGCTT 1 cut(s) 1886
HinfI GANTC 8 cut(s) 438, 464, 641, 946, 1079, 1301, 1387, 1771
HphI GGTGA 4 cut(s) 539, 805, 1507, 2087
Hpy166II GTNNAC 7 cut(s) 91, 131, 217, 291, 829, 940, 1265
Hpy188I TCNGA 6 cut(s) 730, 931, 1240, 1594, 1837, 2022
Hpy188III TCNNGA 4 cut(s) 43, 399, 999, 1664
Hpy8I GTNNAC 7 cut(s) 91, 131, 217, 291, 829, 940, 1265
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 1222, 1480
HpyCH4III ACNGT 7 cut(s) 95, 184, 700, 902, 1100, 2067, 2090
HpyCH4IV ACGT 4 cut(s) 87, 516, 542, 1545
HpyF10VI GCNNNNNNNGC 8 cut(s) 352, 506, 1022, 1217, 1657, 1745, 1810, 1964
HpyF3I CTNAG 6 cut(s) 251, 630, 1216, 1237, 1286, 1986
HpySE526I ACGT 4 cut(s) 87, 516, 542, 1545
HspAI GCGC 4 cut(s) 34, 1401, 1651, 1653
Ksp22I TGATCA 2 cut(s) 1201, 1504
LguI GCTCTTC 2 cut(s) 333, 1803
LmnI GCTCC 4 cut(s) 74, 565, 607, 1618
Lsp1109I GCAGC 4 cut(s) 358, 546, 592, 827
LweI GCATC 9 cut(s) 436, 616, 921, 1144, 1354, 1556, 1669, 1699, 1855
MabI ACCWGGT 1 cut(s) 150
MaeI CTAG 9 cut(s) 812, 909, 989, 1320, 1368, 1664, 1793, 1799, 1875
MaeII ACGT 4 cut(s) 87, 516, 542, 1545
MaeIII GTNAC 3 cut(s) 512, 793, 1043
MfeI CAATTG 2 cut(s) 597, 1602
MflI RGATCY 3 cut(s) 654, 956, 1639
MhlI GDGCHC 3 cut(s) 328, 409, 1815
MlyI GAGTC 4 cut(s) 447, 650, 1381, 1780
MmeI TCCRAC 2 cut(s) 1904, 1910
Mph1103I ATGCAT 2 cut(s) 451, 1960
MseI TTAA 7 cut(s) 309, 369, 672, 1019, 1092, 1854, 1962
MspA1I CMGCKG 2 cut(s) 583, 1220
MspCI CTTAAG 1 cut(s) 368
MspR9I CCNGG 3 cut(s) 152, 1259, 1920
MunI CAATTG 2 cut(s) 597, 1602
Mva1269I GAATGC 3 cut(s) 31, 355, 502
MvaI CCWGG 3 cut(s) 152, 1259, 1920
MvnI CGCG 4 cut(s) 122, 239, 425, 1653
MwoI GCNNNNNNNGC 8 cut(s) 352, 506, 1022, 1217, 1657, 1745, 1810, 1964
NdeI CATATG 2 cut(s) 451, 1176
NheI GCTAGC 1 cut(s) 811
NlaIV GGNNCC 3 cut(s) 76, 1249, 1738
NmuCI GTSAC 1 cut(s) 793
NsbI TGCGCA 1 cut(s) 35
NsiI ATGCAT 2 cut(s) 451, 1960
NspI RCATGY 5 cut(s) 421, 871, 1073, 1843, 1958
PaeI GCATGC 1 cut(s) 1958
PaqCI CACCTGC 1 cut(s) 515
PauI GCGCGC 1 cut(s) 1651
PciI ACATGT 3 cut(s) 417, 1069, 1839
PciSI GCTCTTC 2 cut(s) 333, 1803
PctI GAATGC 3 cut(s) 31, 355, 502
PfeI GAWTC 4 cut(s) 464, 946, 1079, 1301
PkrI GCNGC 6 cut(s) 238, 348, 474, 536, 582, 817
PleI GAGTC 4 cut(s) 446, 649, 1381, 1779
PmaCI CACGTG 1 cut(s) 1546
PmlI CACGTG 1 cut(s) 1546
PpsI GAGTC 4 cut(s) 446, 649, 1381, 1779
Ppu21I YACGTR 2 cut(s) 517, 1546
PscI ACATGT 3 cut(s) 417, 1069, 1839
Psp6I CCWGG 3 cut(s) 150, 1257, 1918
PspCI CACGTG 1 cut(s) 1546
PspFI CCCAGC 2 cut(s) 682, 1655
PspGI CCWGG 3 cut(s) 150, 1257, 1918
PspN4I GGNNCC 3 cut(s) 76, 1249, 1738
PspPI GGNCC 3 cut(s) 1001, 1096, 1465
PstI CTGCAG 3 cut(s) 59, 582, 1745
PsuI RGATCY 3 cut(s) 654, 956, 1639
PteI GCGCGC 1 cut(s) 1651
PvuII CAGCTG 2 cut(s) 583, 1220
RsaI GTAC 7 cut(s) 119, 422, 651, 697, 821, 939, 1224
RsaNI GTAC 7 cut(s) 118, 421, 650, 696, 820, 938, 1223
SapI GCTCTTC 2 cut(s) 333, 1803
SaqAI TTAA 7 cut(s) 309, 369, 672, 1019, 1092, 1854, 1962
SatI GCNGC 6 cut(s) 237, 347, 473, 535, 581, 816
Sau96I GGNCC 3 cut(s) 1001, 1096, 1465
SbfI CCTGCAGG 1 cut(s) 1745
SchI GAGTC 4 cut(s) 447, 650, 1381, 1780
ScrFI CCNGG 3 cut(s) 152, 1259, 1920
SdaI CCTGCAGG 1 cut(s) 1745
SduI GDGCHC 3 cut(s) 328, 409, 1815
SexAI ACCWGGT 1 cut(s) 150
SfaNI GCATC 9 cut(s) 436, 616, 921, 1144, 1354, 1556, 1669, 1699, 1855
SfcI CTRYAG 4 cut(s) 55, 578, 898, 1741
SinI GGWCC 3 cut(s) 1001, 1096, 1465
SmlI CTYRAG 3 cut(s) 368, 1034, 1159
SmoI CTYRAG 3 cut(s) 368, 1034, 1159
SphI GCATGC 1 cut(s) 1958
Sse8387I CCTGCAGG 1 cut(s) 1745
SsiI CCGC 4 cut(s) 222, 237, 473, 1350
SspI AATATT 1 cut(s) 1759
SspMI CTAG 9 cut(s) 812, 909, 989, 1320, 1368, 1664, 1793, 1799, 1875
StyD4I CCNGG 3 cut(s) 150, 1257, 1918
StyI CCWWGG 5 cut(s) 78, 360, 645, 1053, 1083
TaaI ACNGT 7 cut(s) 95, 184, 700, 902, 1100, 2067, 2090
TaiI ACGT 4 cut(s) 90, 519, 545, 1548
TaqI TCGA 5 cut(s) 44, 159, 231, 331, 441
TaqII GACCGA 1 cut(s) 1218
TatI WGTACW 1 cut(s) 1222
TauI GCSGC 2 cut(s) 239, 475
TfiI GAWTC 4 cut(s) 464, 946, 1079, 1301
Tru1I TTAA 7 cut(s) 309, 369, 672, 1019, 1092, 1854, 1962
Tru9I TTAA 7 cut(s) 309, 369, 672, 1019, 1092, 1854, 1962
TscAI CASTG 6 cut(s) 59, 925, 1467, 1820, 1994, 2095
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 4 cut(s) 346, 534, 580, 815
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 5 cut(s) 953, 1164, 1262, 1496, 1601
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 6 cut(s) 59, 925, 1467, 1820, 1994, 2095
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 3 cut(s) 1001, 1096, 1465
XapI RAATTY 4 cut(s) 802, 1414, 1579, 2016
XbaI TCTAGA 1 cut(s) 1663
XceI RCATGY 5 cut(s) 421, 871, 1073, 1843, 1958
XmiI GTMKAC 1 cut(s) 290
XspI CTAG 9 cut(s) 812, 909, 989, 1320, 1368, 1664, 1793, 1799, 1875
Zsp2I ATGCAT 2 cut(s) 451, 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.