Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0011106

Physical Location & Seq

Genomic Coordinates Forward (+)

21647833 .. 21654097

Gene Structure 6265 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00349040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGAAATTTCTAAGCGTTATGGGAAACTCTTTCGGATGCTCAGCATCTGGGGAGCGGTTAGTTTCAGCTGCAAGAGATGGAGATCTTCAAGAGGCCAAGGCCTTGTTGGAATGTAATCCTCGTCTAGCAAGGTACTCCACTTTTGGTGTTCGTAATTCTCCACTCCATTATTCTGCAGCTCAGGGCCACCATGAGATAGTTTCACTCTTGCTTGAGTCTGGAGTTGATATTAATCTCAGGAATTATCGTGGTCAGACTGCTTTGATGCAAGCTTGCCAACATGGTCATTGGGAGGTTGTTCTGACCCTGATTCTGTTTAAAGCCACCATTCACAGAGCAGATTATCTGAATGGGGGTACTGCACTCCACTTTGCTGCTTTGAATGGTCATACTCGGTGCATCAGAATCCTCCTTGCTGATTACATTCCCAGCTCTCCAGATTTTTGGAGTATGTTAAGGAATGAATTAGAGAATGAAGAACCAATTACAGAGTTTGATCATGATGTTTTGGGTCAGATAATTAATAGGCCCGCTGATGGAGGAATCACAGCTCTGCATATGGCAGCACTAAATGGTCATCTTGAAACTGTACAACTACTTTTGGATTTAGGGGCTTCTGTTTTTGCAATTACTGTGGAAGATGGAGCTACAATTGATTTAATAGGGGCTGGAAGCACAGCCCTACATTATGCTGCATGTGGAGGAAATGCCCAGTGTTGTCAAATTTTGATTTCCAGGGGTGCCAGTCTGAAAGCTGAAAATGCAAACCGATGGACGCCCATGATGGTGGCTCGTTCATGGCATAGAGATTGGCTTGAGGAAGTCCTTAGCACGGAGCAAGAAGGCCAGTCACAGATACTTCCTAGTCTTCCTTCACCATACCTATCCCTTCCCCTAATGAGCATTGTGAAGATTGCTAGGCAAATCAGAGAATGTGGATGGAGGAACAATGATTCTTTACCTGCATGCGAAGATCCATGCGTTGTCTGTTTGGAAAGACAGTGCACCGTGGCTGCAGAAGTTAAGTGCTCACTAGGTTGTGATCATGAGTTCTGCATAAGTTGTGCCCTATACCTTTGTTCGACAAACTGCAGCGCGGTTGTGGCGCGTGGTCCCTTGGGATCAATCGCATGTCCTCTTTGCCGGAATGGCATAGTTTCATTTGCGAAGCTTCTAAAAACAAGGCCAACGGTGAAGGGGATGGCAAAAACTAGTTTATCCTTATCATTTTGCCGCTGTTCTACCGAGGAACCAGAGAGAACCTCCTCAATAACCACCCCTTTATGCAAACCCAGCTTTCGTTCCTCTCGGAAATGTTCGCTGGGATATTTCCGGTCCCAGAGCTTCCAGAAGTTCCCATCTATCAAACTCAACTACGGCCTTTGCATGGGAGGTGTTTCAAACATTACACCTTGTTTGGTTCCGTGCTCTGCTCGTAGATGTTTGAGATCAGGCTTTAGACGAACAACTTCTCAGCCTGAGGGCAGAAGGTCATGGCTATCTGCACTCAATCAACAAGCAATGACTAGCAGCGGATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006521 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010364 GO:0010366 GO:0010565 GO:0010817 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022622 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031335 GO:0031336 GO:0032350 GO:0032351 GO:0032353 GO:0032446 GO:0032501 GO:0032502 GO:0033238 GO:0033239 GO:0036211 GO:0042221 GO:0042762 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0045763 GO:0046885 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051175 GO:0051603 GO:0051865 GO:0062012 GO:0062014 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080090 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1900908 GO:1900909 GO:1900911 GO:1900912 GO:1901564 GO:1901565 GO:1901575 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4

Protein Analysis

513

Amino Acids

55.99

Weight (kDa)

8.28

Isoelectric Point (pI)

50.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	21 - 104	9.7e-15	Ankyrin repeats (3 copies)
Ank	PF00023	52 - 81	2.6e-07	Ankyrin repeat
Ank_4	PF13637	55 - 104	7.4e-10	Ankyrin repeats (many copies)
Ank_2	PF12796	114 - 207	1.3e-10	Ankyrin repeats (3 copies)
Ank	PF00023	181 - 210	4.7e-07	Ankyrin repeat
Ank_2	PF12796	223 - 273	1.7e-07	Ankyrin repeats (3 copies)
Ank	PF00023	224 - 254	6.4e-06	Ankyrin repeat
RING_XB3-XBAT31	PF24921	327 - 387	1.5e-12	E3 ubiquitin-protein ligase XB3/XBAT31 RING finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011106)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57740
fragaria_vesca	FvH4_4g09590
malus_domestica	MD00G1085900.v1.1 MD16G1262900.v1.1
prunus_persica	Prupe.1G097200_v2.0.a1 Prupe.1G097200_v2.0.a1 Prupe.1G097200_v2.0.a1 Prupe.1G097200_v2.0.a1
pyrus_communis	pycom12494g00060
rosa_chinensis	RchiOBHm_Chr4g0404891
rosa_laevigata	RLG00000008896
rosa_multiflora	Rmu_sc0001179.1_g000005
rosa_roxburghii	Rroxscaffold_5G00349040
rosa_rugosa	Rorug04G0049700
rosa_samantha	Rh4AG125900 Rh4BG119700 Rh4BG119800 Rh4CG134100 Rh4DG119400
rosa_wichuraiana	Rw4G010180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	970
AccB1I	GGYRCC	1 cut(s)	740
AccBSI	CCGCTC	1 cut(s)	55
AccII	CGCG	2 cut(s)	1097, 1108
AciI	CCGC	5 cut(s)	55, 531, 1097, 1234, 1533
AclWI	GGATC	2 cut(s)	968, 1129
AcsI	RAATTY	2 cut(s)	5, 723
AcyI	GRCGYC	1 cut(s)	776
AfaI	GTAC	3 cut(s)	134, 358, 591
AfiI	CCNNNNNNNGG	3 cut(s)	536, 832, 1387
AgsI	TTSAA	4 cut(s)	89, 382, 584, 1401
AhlI	ACTAGT	1 cut(s)	1211
AjnI	CCWGG	1 cut(s)	734
Alw21I	GWGCWC	3 cut(s)	1007, 1031, 1430
Alw44I	GTGCAC	1 cut(s)	1003
AlwI	GGATC	2 cut(s)	968, 1129
AlwNI	CAGNNNCTG	1 cut(s)	47
AoxI	GGCC	7 cut(s)	93, 99, 184, 527, 844, 1184, 1378
ApaLI	GTGCAC	1 cut(s)	1003
ApeKI	GCWGC	8 cut(s)	68, 176, 374, 563, 692, 1013, 1092, 1530
ApoI	RAATTY	2 cut(s)	5, 723
AseI	ATTAAT	2 cut(s)	231, 522
Asp700I	GAANNNNTTC	1 cut(s)	1468
AspLEI	GCGC	2 cut(s)	1097, 1108
AspS9I	GGNCC	4 cut(s)	184, 528, 1112, 1335
AsuHPI	GGTGA	2 cut(s)	867, 1204
AvaII	GGWCC	2 cut(s)	1112, 1335
AxyI	CCTNAGG	1 cut(s)	1479
BaeGI	GKGCMC	2 cut(s)	1007, 1069
BanI	GGYRCC	1 cut(s)	740
BbsI	GAAGAC	1 cut(s)	860
Bbv12I	GWGCWC	3 cut(s)	1007, 1031, 1430
BbvI	GCAGC	7 cut(s)	55, 188, 361, 575, 679, 1000, 1104
BccI	CCATC	8 cut(s)	71, 530, 635, 765, 778, 933, 1195, 1366
BceAI	ACGGC	1 cut(s)	1393
BciT130I	CCWGG	1 cut(s)	736
BclI	TGATCA	2 cut(s)	496, 1042
BcuI	ACTAGT	1 cut(s)	1211
BfaI	CTAG	6 cut(s)	125, 864, 918, 1034, 1212, 1527
BfmI	CTRYAG	3 cut(s)	174, 1014, 1090
BfuAI	ACCTGC	1 cut(s)	970
BglI	GCCNNNNNGGC	1 cut(s)	1149
BglII	AGATCT	1 cut(s)	82
BisI	GCNGC	9 cut(s)	69, 177, 375, 564, 693, 1014, 1093, 1234, 1531
BlpI	GCTNAGC	1 cut(s)	40
BlsI	GCNGC	9 cut(s)	70, 178, 376, 565, 694, 1015, 1094, 1235, 1532
Bme1390I	CCNGG	1 cut(s)	736
Bme18I	GGWCC	2 cut(s)	1112, 1335
BmgT120I	GGNCC	4 cut(s)	184, 528, 1112, 1335
BmiI	GGNNCC	5 cut(s)	742, 1114, 1251, 1337, 1422
BmrFI	CCNGG	1 cut(s)	736
BmrI	ACTGGG	1 cut(s)	706
BmsI	GCATC	5 cut(s)	26, 53, 255, 408, 1526
BmuI	ACTGGG	1 cut(s)	706
BpiI	GAAGAC	1 cut(s)	860
BplI	GAGNNNNNCTC	2 cut(s)	1247, 1279
BpmI	CTGGAG	2 cut(s)	240, 420
Bpu10I	CCTNAGC	2 cut(s)	180, 827
Bpu1102I	GCTNAGC	1 cut(s)	40
BpuEI	CTTGAG	2 cut(s)	233, 836
BsaBI	GATNNNNATC	2 cut(s)	81, 231
BsaHI	GRCGYC	1 cut(s)	776
BsaJI	CCNNGG	5 cut(s)	96, 735, 1008, 1116, 1245
BsaWI	WCCGGW	1 cut(s)	1332
Bsc4I	CCNNNNNNNGG	3 cut(s)	536, 832, 1387
Bse1I	ACTGG	3 cut(s)	712, 744, 847
Bse21I	CCTNAGG	1 cut(s)	1479
Bse3DI	GCAATG	1 cut(s)	1527
Bse8I	GATNNNNATC	2 cut(s)	81, 231
BseBI	CCWGG	1 cut(s)	736
BseDI	CCNNGG	5 cut(s)	96, 735, 1008, 1116, 1245
BseGI	GGATG	4 cut(s)	41, 944, 1206, 1541
BseJI	GATNNNNATC	2 cut(s)	81, 231
BseLI	CCNNNNNNNGG	3 cut(s)	536, 832, 1387
BseMI	GCAATG	1 cut(s)	1527
BseMII	CTCAG	5 cut(s)	54, 194, 250, 1470, 1487
BseNI	ACTGG	3 cut(s)	712, 744, 847
BseRI	GAGGAG	1 cut(s)	1255
BseSI	GKGCMC	2 cut(s)	1007, 1069
BseXI	GCAGC	7 cut(s)	55, 188, 361, 575, 679, 1000, 1104
BseYI	CCCAGC	3 cut(s)	428, 1292, 1321
BsgI	GTGCAG	2 cut(s)	345, 1488
Bsh1236I	CGCG	2 cut(s)	1097, 1108
BshFI	GGCC	7 cut(s)	95, 101, 186, 529, 846, 1186, 1380
BshNI	GGYRCC	1 cut(s)	740
BsiHKAI	GWGCWC	3 cut(s)	1007, 1031, 1430
BsiSI	CCGG	2 cut(s)	1144, 1333
BslFI	GGGAC	2 cut(s)	1098, 1321
BslI	CCNNNNNNNGG	3 cut(s)	536, 832, 1387
BsmFI	GGGAC	2 cut(s)	1098, 1321
BsnI	GGCC	7 cut(s)	95, 101, 186, 529, 846, 1186, 1380
Bsp1286I	GDGCHC	4 cut(s)	1007, 1031, 1069, 1430
Bsp1407I	TGTACA	1 cut(s)	589
Bsp143I	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
Bsp1720I	GCTNAGC	1 cut(s)	40
BspACI	CCGC	5 cut(s)	55, 531, 1097, 1234, 1533
BspANI	GGCC	7 cut(s)	95, 101, 186, 529, 846, 1186, 1380
BspCNI	CTCAG	5 cut(s)	53, 193, 249, 1471, 1486
BspFNI	CGCG	2 cut(s)	1097, 1108
BspHI	TCATGA	2 cut(s)	499, 1045
BspLI	GGNNCC	5 cut(s)	742, 1114, 1251, 1337, 1422
BspMAI	CTGCAG	3 cut(s)	178, 1018, 1094
BspMI	ACCTGC	1 cut(s)	970
BspPI	GGATC	2 cut(s)	968, 1129
BspT107I	GGYRCC	1 cut(s)	740
BsrBI	CCGCTC	1 cut(s)	55
BsrDI	GCAATG	1 cut(s)	1527
BsrGI	TGTACA	1 cut(s)	589
BsrI	ACTGG	3 cut(s)	712, 744, 847
BssECI	CCNNGG	5 cut(s)	96, 735, 1008, 1116, 1245
BssMI	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
BssNI	GRCGYC	1 cut(s)	776
BssT1I	CCWWGG	2 cut(s)	96, 1116
Bst2UI	CCWGG	1 cut(s)	736
Bst4CI	ACNGT	5 cut(s)	589, 634, 1002, 1009, 1192
BstACI	GRCGYC	1 cut(s)	776
BstAPI	GCANNNNNTGC	1 cut(s)	1536
BstAUI	TGTACA	1 cut(s)	589
BstC8I	GCNNGC	4 cut(s)	270, 274, 531, 967
BstDEI	CTNAG	7 cut(s)	11, 40, 180, 236, 827, 1473, 1479
BstDSI	CCRYGG	1 cut(s)	1008
BstF5I	GGATG	4 cut(s)	41, 944, 1206, 1541
BstFNI	CGCG	2 cut(s)	1097, 1108
BstHHI	GCGC	2 cut(s)	1097, 1108
BstKTI	GATC	6 cut(s)	85, 499, 976, 1045, 1124, 1451
BstMBI	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
BstMWI	GCNNNNNNNGC	5 cut(s)	761, 1103, 1149, 1293, 1536
BstNI	CCWGG	1 cut(s)	736
BstNSI	RCATGY	3 cut(s)	699, 969, 1134
BstSCI	CCNGG	1 cut(s)	734
BstSFI	CTRYAG	3 cut(s)	174, 1014, 1090
BstSLI	GKGCMC	2 cut(s)	1007, 1069
BstUI	CGCG	2 cut(s)	1097, 1108
BstV1I	GCAGC	7 cut(s)	55, 188, 361, 575, 679, 1000, 1104
BstV2I	GAAGAC	1 cut(s)	860
BstX2I	RGATCY	2 cut(s)	82, 973
BstXI	CCANNNNNNTGG	2 cut(s)	444, 787
BstYI	RGATCY	2 cut(s)	82, 973
Bsu36I	CCTNAGG	1 cut(s)	1479
BsuRI	GGCC	7 cut(s)	95, 101, 186, 529, 846, 1186, 1380
BtgI	CCRYGG	1 cut(s)	1008
BtsCI	GGATG	4 cut(s)	41, 944, 1206, 1541
BtsIMutI	CAGTG	2 cut(s)	719, 1007
BveI	ACCTGC	1 cut(s)	970
Cac8I	GCNNGC	4 cut(s)	270, 274, 531, 967
CaiI	CAGNNNCTG	1 cut(s)	47
CciI	TCATGA	2 cut(s)	499, 1045
CfoI	GCGC	2 cut(s)	1097, 1108
Cfr13I	GGNCC	4 cut(s)	184, 528, 1112, 1335
CseI	GACGC	1 cut(s)	784
Csp6I	GTAC	3 cut(s)	133, 357, 590
CspCI	CAANNNNNGTGG	2 cut(s)	615, 650
CviQI	GTAC	3 cut(s)	133, 357, 590
DdeI	CTNAG	7 cut(s)	11, 40, 180, 236, 827, 1473, 1479
DpnI	GATC	6 cut(s)	84, 498, 975, 1044, 1123, 1450
DpnII	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
DraI	TTTAAA	1 cut(s)	319
Eco130I	CCWWGG	2 cut(s)	96, 1116
Eco147I	AGGCCT	1 cut(s)	101
Eco47I	GGWCC	2 cut(s)	1112, 1335
Eco81I	CCTNAGG	1 cut(s)	1479
EcoRII	CCWGG	1 cut(s)	734
EcoT14I	CCWWGG	2 cut(s)	96, 1116
ErhI	CCWWGG	2 cut(s)	96, 1116
FaqI	GGGAC	2 cut(s)	1098, 1321
FauI	CCCGC	1 cut(s)	538
FauNDI	CATATG	1 cut(s)	558
FbaI	TGATCA	2 cut(s)	496, 1042
Fnu4HI	GCNGC	9 cut(s)	69, 177, 375, 564, 693, 1014, 1093, 1234, 1531
FokI	GGATG	3 cut(s)	48, 951, 1213
Fsp4HI	GCNGC	9 cut(s)	69, 177, 375, 564, 693, 1014, 1093, 1234, 1531
FspBI	CTAG	6 cut(s)	125, 864, 918, 1034, 1212, 1527
GlaI	GCGC	2 cut(s)	1096, 1107
GluI	GCNGC	9 cut(s)	69, 177, 375, 564, 693, 1014, 1093, 1234, 1531
GsaI	CCCAGC	3 cut(s)	432, 1296, 1325
GsuI	CTGGAG	2 cut(s)	240, 420
HaeIII	GGCC	7 cut(s)	95, 101, 186, 529, 846, 1186, 1380
HapII	CCGG	2 cut(s)	1144, 1333
HgaI	GACGC	1 cut(s)	784
HhaI	GCGC	2 cut(s)	1097, 1108
Hin1I	GRCGYC	1 cut(s)	776
Hin6I	GCGC	2 cut(s)	1095, 1106
HinP1I	GCGC	2 cut(s)	1095, 1106
HindIII	AAGCTT	2 cut(s)	270, 1169
HinfI	GANTC	5 cut(s)	215, 310, 405, 543, 953
HpaII	CCGG	2 cut(s)	1144, 1333
HphI	GGTGA	2 cut(s)	867, 1204
Hpy166II	GTNNAC	1 cut(s)	1005
Hpy188I	TCNGA	9 cut(s)	35, 255, 303, 348, 404, 516, 750, 929, 1311
Hpy188III	TCNNGA	8 cut(s)	89, 219, 238, 437, 500, 581, 1046, 1348
Hpy8I	GTNNAC	1 cut(s)	1005
HpyAV	CCTTC	5 cut(s)	836, 882, 899, 1189, 1482
HpyCH4III	ACNGT	5 cut(s)	589, 634, 1002, 1009, 1192
HpyF10VI	GCNNNNNNNGC	5 cut(s)	761, 1103, 1149, 1293, 1536
HpyF3I	CTNAG	7 cut(s)	11, 40, 180, 236, 827, 1473, 1479
Hsp92I	GRCGYC	1 cut(s)	776
HspAI	GCGC	2 cut(s)	1095, 1106
Ksp22I	TGATCA	2 cut(s)	496, 1042
Kzo9I	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
LmnI	GCTCC	3 cut(s)	52, 644, 835
Lsp1109I	GCAGC	7 cut(s)	55, 188, 361, 575, 679, 1000, 1104
LweI	GCATC	5 cut(s)	26, 53, 255, 408, 1526
MaeI	CTAG	6 cut(s)	125, 864, 918, 1034, 1212, 1527
MaeIII	GTNAC	1 cut(s)	849
MalI	GATC	6 cut(s)	84, 498, 975, 1044, 1123, 1450
MbiI	CCGCTC	1 cut(s)	55
MboI	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
MboII	GAAGA	6 cut(s)	77, 488, 650, 860, 922, 983
MfeI	CAATTG	1 cut(s)	651
MflI	RGATCY	2 cut(s)	82, 973
MhlI	GDGCHC	4 cut(s)	1007, 1031, 1069, 1430
MluCI	AATT	9 cut(s)	5, 154, 241, 464, 483, 519, 627, 651, 723
MlyI	GAGTC	1 cut(s)	224
MmeI	TCCRAC	1 cut(s)	87
MroXI	GAANNNNTTC	1 cut(s)	1468
MseI	TTAA	6 cut(s)	231, 318, 455, 522, 659, 1023
MslI	CAYNNNNRTG	1 cut(s)	785
MspA1I	CMGCKG	4 cut(s)	68, 533, 1236, 1533
MspI	CCGG	2 cut(s)	1144, 1333
MspR9I	CCNGG	1 cut(s)	736
MunI	CAATTG	1 cut(s)	651
MvaI	CCWGG	1 cut(s)	736
MvnI	CGCG	2 cut(s)	1097, 1108
MwoI	GCNNNNNNNGC	5 cut(s)	761, 1103, 1149, 1293, 1536
NdeI	CATATG	1 cut(s)	558
NdeII	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
NlaIV	GGNNCC	5 cut(s)	742, 1114, 1251, 1337, 1422
NmuCI	GTSAC	1 cut(s)	849
NspI	RCATGY	3 cut(s)	699, 969, 1134
PaeI	GCATGC	1 cut(s)	969
PagI	TCATGA	2 cut(s)	499, 1045
PceI	AGGCCT	1 cut(s)	101
PdmI	GAANNNNTTC	1 cut(s)	1468
PfeI	GAWTC	4 cut(s)	310, 405, 543, 953
PkrI	GCNGC	9 cut(s)	70, 178, 376, 565, 694, 1015, 1094, 1235, 1532
PleI	GAGTC	1 cut(s)	223
PpsI	GAGTC	1 cut(s)	223
PshBI	ATTAAT	2 cut(s)	231, 522
Psp6I	CCWGG	1 cut(s)	734
PspFI	CCCAGC	3 cut(s)	428, 1292, 1321
PspGI	CCWGG	1 cut(s)	734
PspN4I	GGNNCC	5 cut(s)	742, 1114, 1251, 1337, 1422
PspPI	GGNCC	4 cut(s)	184, 528, 1112, 1335
PstI	CTGCAG	3 cut(s)	178, 1018, 1094
PstNI	CAGNNNCTG	1 cut(s)	47
PsuI	RGATCY	2 cut(s)	82, 973
PvuII	CAGCTG	1 cut(s)	68
RsaI	GTAC	3 cut(s)	134, 358, 591
RsaNI	GTAC	3 cut(s)	133, 357, 590
RseI	CAYNNNNRTG	1 cut(s)	785
SaqAI	TTAA	6 cut(s)	231, 318, 455, 522, 659, 1023
SatI	GCNGC	9 cut(s)	69, 177, 375, 564, 693, 1014, 1093, 1234, 1531
Sau3AI	GATC	6 cut(s)	82, 496, 973, 1042, 1121, 1448
Sau96I	GGNCC	4 cut(s)	184, 528, 1112, 1335
SchI	GAGTC	1 cut(s)	224
ScrFI	CCNGG	1 cut(s)	736
SduI	GDGCHC	4 cut(s)	1007, 1031, 1069, 1430
SfaNI	GCATC	5 cut(s)	26, 53, 255, 408, 1526
SfcI	CTRYAG	3 cut(s)	174, 1014, 1090
SinI	GGWCC	2 cut(s)	1112, 1335
SmiMI	CAYNNNNRTG	1 cut(s)	785
SmlI	CTYRAG	2 cut(s)	212, 815
SmoI	CTYRAG	2 cut(s)	212, 815
SpeI	ACTAGT	1 cut(s)	1211
SphI	GCATGC	1 cut(s)	969
Sse9I	AATT	9 cut(s)	5, 154, 241, 464, 483, 519, 627, 651, 723
SseBI	AGGCCT	1 cut(s)	101
SsiI	CCGC	5 cut(s)	55, 531, 1097, 1234, 1533
SspMI	CTAG	6 cut(s)	125, 864, 918, 1034, 1212, 1527
StuI	AGGCCT	1 cut(s)	101
StyD4I	CCNGG	1 cut(s)	734
StyI	CCWWGG	2 cut(s)	96, 1116
TaaI	ACNGT	5 cut(s)	589, 634, 1002, 1009, 1192
TaqI	TCGA	1 cut(s)	1082
TasI	AATT	9 cut(s)	5, 154, 241, 464, 483, 519, 627, 651, 723
TatI	WGTACW	1 cut(s)	589
TauI	GCSGC	1 cut(s)	1236
TfiI	GAWTC	4 cut(s)	310, 405, 543, 953
Tru1I	TTAA	6 cut(s)	231, 318, 455, 522, 659, 1023
Tru9I	TTAA	6 cut(s)	231, 318, 455, 522, 659, 1023
TscAI	CASTG	2 cut(s)	719, 1007
TseFI	GTSAC	1 cut(s)	849
TseI	GCWGC	8 cut(s)	68, 176, 374, 563, 692, 1013, 1092, 1530
Tsp45I	GTSAC	1 cut(s)	849
TspDTI	ATGAA	5 cut(s)	17, 477, 489, 786, 1149
TspGWI	ACGGA	2 cut(s)	848, 1413
TspRI	CASTG	2 cut(s)	719, 1007
VneI	GTGCAC	1 cut(s)	1003
VpaK11BI	GGWCC	2 cut(s)	1112, 1335
VspI	ATTAAT	2 cut(s)	231, 522
XapI	RAATTY	2 cut(s)	5, 723
XceI	RCATGY	3 cut(s)	699, 969, 1134
XcmI	CCANNNNNNNNNTGG	1 cut(s)	103
XmnI	GAANNNNTTC	1 cut(s)	1468
XspI	CTAG	6 cut(s)	125, 864, 918, 1034, 1212, 1527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.