Rroxscaffold_5G00351060

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017826
Physical Location & Seq
Reverse (-)
25946261 .. 25973669
27409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351060.1

Sequence Viewer

Length: 1236 bp
ATGAATGAGGACAGGTTTGAAAATGATGACTCTGGTTCTAGAGTCTCAGATTTCTCTATGTCAAGTGGAGGTGAGTACAGTCTTCTAAATTTCAGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGCAAATGTGTTCCCTGGGACATGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACAATCACTCTGTATCCATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGGAGCAGTTGATAATGTCGCAGACTTGGCTGCATCCATAGCAGCATGTCTAAATATTCTGCTAGGAACCCCATCGCCTGAAAATGTGGATGGAGACATTACTTGTGATGATACGTTAAAATGGAAGTGGGTGGAAACATTTCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGCGTTGAGGACTTAAGAAAGTTTTCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGACTATGATATGGACACTTCGTCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCATGTTCATCTGCTGATGGACGTACACTTTTGGAATCTTCTAAGACTTCCTTGGATAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCATTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACTGCTGGAGCATATAGTCTTCTTGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTATCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACATACAGAGTTGGCCTTGAAGTACGTCAATCGTGCGCTGTATCTTTTGCACCTAACATGTGGTCCATCTCATCCAAACACGGCTGCCACCTATATCAATGTAGCAATGATGGAAGAAGGTTTGGGGAATGTCCATGTTGCTCTTAGGTACCTCCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGAGAGAAAAGAATCTTTCAGTCCAGTTGAAGAAATGGAAGATGGAGATTTGGCATTGCTTGATATTGGAGCTAAATGGCAGAATGAATACTAAGCTCGGCTATGGAAACCTTGCCTGGTGGAACTCTTGGAAACAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

411

Amino Acids

46.56

Weight (kDa)

6.81

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 34 - 170 1.4e-20 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 211 - 271 4.2e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 211 - 310 3.9e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 240 - 310 2.2e-13 Tetratricopeptide repeat
TPR_10 PF13374 240 - 281 3.4e-10 Tetratricopeptide repeat
TPR_12 PF13424 286 - 353 2.5e-09 Tetratricopeptide repeat
TPR_10 PF13374 324 - 364 6.8e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1038
AccB1I GGYRCC 1 cut(s) 1038
AccB7I CCANNNNNTGG 1 cut(s) 208
AcsI RAATTY 1 cut(s) 88
AfaI GTAC 7 cut(s) 77, 186, 566, 601, 750, 914, 1040
AfiI CCNNNNNNNGG 1 cut(s) 208
AflII CTTAAG 1 cut(s) 439
AflIII ACRYGT 2 cut(s) 151, 947
AgsI TTSAA 6 cut(s) 20, 161, 397, 890, 910, 1123
AhdI GACNNNNNGTC 1 cut(s) 520
AjnI CCWGG 2 cut(s) 144, 1208
AluBI AGCT 9 cut(s) 175, 242, 490, 823, 869, 1052, 1082, 1165, 1189
AluI AGCT 9 cut(s) 175, 242, 490, 823, 869, 1052, 1082, 1165, 1189
Alw26I GTCTC 3 cut(s) 49, 336, 528
AoxI GGCC 3 cut(s) 481, 701, 903
ApeKI GCWGC 3 cut(s) 278, 290, 974
ApoI RAATTY 1 cut(s) 88
AseI ATTAAT 1 cut(s) 810
Asp700I GAANNNNTTC 2 cut(s) 387, 448
Asp718I GGTACC 1 cut(s) 1038
AspLEI GCGC 1 cut(s) 928
AspS9I GGNCC 3 cut(s) 556, 701, 953
AsuHPI GGTGA 1 cut(s) 83
AvaII GGWCC 2 cut(s) 556, 953
BanI GGYRCC 1 cut(s) 1038
BbsI GAAGAC 2 cut(s) 74, 728
BbvI GCAGC 3 cut(s) 265, 302, 961
BccI CCATC 9 cut(s) 202, 328, 332, 402, 587, 791, 964, 994, 1129
BceAI ACGGC 1 cut(s) 987
BciT130I CCWGG 2 cut(s) 146, 1210
BciVI GTATCC 1 cut(s) 209
BclI TGATCA 2 cut(s) 832, 1084
BcoDI GTCTC 3 cut(s) 49, 336, 528
BfaI CTAG 2 cut(s) 39, 311
BfmI CTRYAG 1 cut(s) 744
BfrI CTTAAG 1 cut(s) 439
BfuI GTATCC 1 cut(s) 209
BisI GCNGC 3 cut(s) 279, 291, 975
BlsI GCNGC 3 cut(s) 280, 292, 976
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 2 cut(s) 146, 1210
Bme18I GGWCC 2 cut(s) 556, 953
BmeRI GACNNNNNGTC 1 cut(s) 520
BmgT120I GGNCC 3 cut(s) 556, 701, 953
BmiI GGNNCC 3 cut(s) 316, 558, 1040
BmrFI CCNGG 2 cut(s) 146, 1210
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 2 cut(s) 290, 640
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 2 cut(s) 74, 728
BpmI CTGGAG 2 cut(s) 665, 744
BpuEI CTTGAG 2 cut(s) 506, 1040
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 4 cut(s) 144, 145, 497, 627
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 3 cut(s) 648, 766, 1117
Bse3DI GCAATG 3 cut(s) 419, 1002, 1147
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 2 cut(s) 146, 1210
BseDI CCNNGG 4 cut(s) 144, 145, 497, 627
BseGI GGATG 6 cut(s) 281, 343, 413, 655, 835, 961
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 3 cut(s) 419, 1002, 1147
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 3 cut(s) 648, 766, 1117
BseXI GCAGC 3 cut(s) 265, 302, 961
BshFI GGCC 3 cut(s) 483, 703, 905
BshNI GGYRCC 1 cut(s) 1038
BslFI GGGAC 3 cut(s) 162, 515, 542
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 3 cut(s) 49, 336, 528
BsmBI CGTCTC 1 cut(s) 528
BsmFI GGGAC 3 cut(s) 162, 515, 542
BsnI GGCC 3 cut(s) 483, 703, 905
Bsp1407I TGTACA 2 cut(s) 184, 564
Bsp143I GATC 2 cut(s) 832, 1084
BspANI GGCC 3 cut(s) 483, 703, 905
BspCNI CTCAG 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 316, 558, 1040
BspT107I GGYRCC 1 cut(s) 1038
BspTI CTTAAG 1 cut(s) 439
BsrDI GCAATG 3 cut(s) 419, 1002, 1147
BsrGI TGTACA 2 cut(s) 184, 564
BsrI ACTGG 3 cut(s) 648, 766, 1117
BssECI CCNNGG 4 cut(s) 144, 145, 497, 627
BssMI GATC 2 cut(s) 832, 1084
BssT1I CCWWGG 2 cut(s) 497, 627
Bst2UI CCWGG 2 cut(s) 146, 1210
Bst4CI ACNGT 2 cut(s) 80, 753
Bst6I CTCTTC 1 cut(s) 392
BstAFI CTTAAG 1 cut(s) 439
BstAUI TGTACA 2 cut(s) 184, 564
BstC8I GCNNGC 2 cut(s) 219, 240
BstDEI CTNAG 5 cut(s) 46, 618, 669, 1034, 1185
BstF5I GGATG 6 cut(s) 281, 343, 413, 655, 835, 961
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 2 cut(s) 835, 1087
BstMAI GTCTC 3 cut(s) 49, 336, 528
BstMBI GATC 2 cut(s) 832, 1084
BstMWI GCNNNNNNNGC 2 cut(s) 275, 287
BstNI CCWGG 2 cut(s) 146, 1210
BstNSI RCATGY 5 cut(s) 155, 297, 575, 582, 951
BstSCI CCNGG 2 cut(s) 144, 1208
BstSFI CTRYAG 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 265, 302, 961
BstV2I GAAGAC 2 cut(s) 74, 728
BstXI CCANNNNNNTGG 1 cut(s) 479
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 483, 703, 905
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 6 cut(s) 281, 343, 413, 655, 835, 961
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 2 cut(s) 219, 240
CfoI GCGC 1 cut(s) 928
Cfr13I GGNCC 3 cut(s) 556, 701, 953
Csp6I GTAC 7 cut(s) 76, 185, 565, 600, 749, 913, 1039
CviQI GTAC 7 cut(s) 76, 185, 565, 600, 749, 913, 1039
DdeI CTNAG 5 cut(s) 46, 618, 669, 1034, 1185
DpnI GATC 2 cut(s) 834, 1086
DpnII GATC 2 cut(s) 832, 1084
DriI GACNNNNNGTC 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 392
Eam1105I GACNNNNNGTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 497, 627
Eco47I GGWCC 2 cut(s) 556, 953
EcoRII CCWGG 2 cut(s) 144, 1208
EcoT14I CCWWGG 2 cut(s) 497, 627
EcoT22I ATGCAT 1 cut(s) 120
ErhI CCWWGG 2 cut(s) 497, 627
Esp3I CGTCTC 1 cut(s) 528
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 3 cut(s) 162, 515, 542
FbaI TGATCA 2 cut(s) 832, 1084
Fnu4HI GCNGC 3 cut(s) 279, 291, 975
FokI GGATG 6 cut(s) 268, 350, 420, 662, 822, 948
Fsp4HI GCNGC 3 cut(s) 279, 291, 975
FspBI CTAG 2 cut(s) 39, 311
GlaI GCGC 1 cut(s) 927
GluI GCNGC 3 cut(s) 279, 291, 975
GsuI CTGGAG 2 cut(s) 665, 744
HaeIII GGCC 3 cut(s) 483, 703, 905
HhaI GCGC 1 cut(s) 928
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HinfI GANTC 4 cut(s) 29, 42, 611, 1105
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 3 cut(s) 565, 602, 641
Hpy188I TCNGA 3 cut(s) 49, 95, 541
Hpy188III TCNNGA 2 cut(s) 39, 839
Hpy8I GTNNAC 3 cut(s) 565, 602, 641
HpyAV CCTTC 2 cut(s) 90, 1001
HpyCH4III ACNGT 2 cut(s) 80, 753
HpyCH4IV ACGT 3 cut(s) 362, 598, 915
HpyCH4V TGCA 8 cut(s) 118, 133, 167, 238, 281, 578, 940, 1062
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 287
HpyF3I CTNAG 5 cut(s) 46, 618, 669, 1034, 1185
HpySE526I ACGT 3 cut(s) 362, 598, 915
HspAI GCGC 1 cut(s) 926
KpnI GGTACC 1 cut(s) 1042
Ksp22I TGATCA 2 cut(s) 832, 1084
Kzo9I GATC 2 cut(s) 832, 1084
LmnI GCTCC 5 cut(s) 180, 251, 725, 1079, 1162
Lsp1109I GCAGC 3 cut(s) 265, 302, 961
LweI GCATC 2 cut(s) 290, 640
MaeI CTAG 2 cut(s) 39, 311
MaeII ACGT 3 cut(s) 362, 598, 915
MaeIII GTNAC 1 cut(s) 854
MalI GATC 2 cut(s) 834, 1086
MboI GATC 2 cut(s) 832, 1084
MboII GAAGA 8 cut(s) 74, 409, 449, 606, 728, 1016, 1135, 1144
MluCI AATT 2 cut(s) 88, 658
MlyI GAGTC 2 cut(s) 23, 51
MnlI CCTC 6 cut(s) 62, 189, 393, 427, 640, 1052
Mph1103I ATGCAT 1 cut(s) 120
MroXI GAANNNNTTC 2 cut(s) 387, 448
MseI TTAA 5 cut(s) 365, 440, 657, 771, 810
MspCI CTTAAG 1 cut(s) 439
MspR9I CCNGG 2 cut(s) 146, 1210
MvaI CCWGG 2 cut(s) 146, 1210
MwoI GCNNNNNNNGC 2 cut(s) 275, 287
NdeII GATC 2 cut(s) 832, 1084
NlaIV GGNNCC 3 cut(s) 316, 558, 1040
NmeAIII GCCGAG 1 cut(s) 1170
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 5 cut(s) 155, 297, 575, 582, 951
PasI CCCWGGG 1 cut(s) 145
PciI ACATGT 2 cut(s) 151, 947
PdmI GAANNNNTTC 2 cut(s) 387, 448
PfeI GAWTC 2 cut(s) 611, 1105
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 3 cut(s) 280, 292, 976
PleI GAGTC 2 cut(s) 23, 50
PpsI GAGTC 2 cut(s) 23, 50
PscI ACATGT 2 cut(s) 151, 947
PshBI ATTAAT 1 cut(s) 810
Psp6I CCWGG 2 cut(s) 144, 1208
PspGI CCWGG 2 cut(s) 144, 1208
PspN4I GGNNCC 3 cut(s) 316, 558, 1040
PspPI GGNCC 3 cut(s) 556, 701, 953
RsaI GTAC 7 cut(s) 77, 186, 566, 601, 750, 914, 1040
RsaNI GTAC 7 cut(s) 76, 185, 565, 600, 749, 913, 1039
SaqAI TTAA 5 cut(s) 365, 440, 657, 771, 810
SatI GCNGC 3 cut(s) 279, 291, 975
Sau3AI GATC 2 cut(s) 832, 1084
Sau96I GGNCC 3 cut(s) 556, 701, 953
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 2 cut(s) 23, 51
ScrFI CCNGG 2 cut(s) 146, 1210
SfaNI GCATC 2 cut(s) 290, 640
SfcI CTRYAG 1 cut(s) 744
SinI GGWCC 2 cut(s) 556, 953
SmlI CTYRAG 3 cut(s) 439, 485, 1055
SmoI CTYRAG 3 cut(s) 439, 485, 1055
Sse9I AATT 2 cut(s) 88, 658
SspI AATATT 1 cut(s) 304
SspMI CTAG 2 cut(s) 39, 311
StyD4I CCNGG 2 cut(s) 144, 1208
StyI CCWWGG 2 cut(s) 497, 627
TaaI ACNGT 2 cut(s) 80, 753
TaiI ACGT 3 cut(s) 365, 601, 918
TaqI TCGA 1 cut(s) 712
TasI AATT 2 cut(s) 88, 658
TatI WGTACW 4 cut(s) 75, 184, 564, 748
TfiI GAWTC 2 cut(s) 611, 1105
Tru1I TTAA 5 cut(s) 365, 440, 657, 771, 810
Tru9I TTAA 5 cut(s) 365, 440, 657, 771, 810
TscAI CASTG 1 cut(s) 766
TseI GCWGC 3 cut(s) 278, 290, 974
TspDTI ATGAA 6 cut(s) 17, 438, 573, 863, 1062, 1193
TspRI CASTG 1 cut(s) 766
Van91I CCANNNNNTGG 1 cut(s) 208
Vha464I CTTAAG 1 cut(s) 439
VpaK11BI GGWCC 2 cut(s) 556, 953
VspI ATTAAT 1 cut(s) 810
XapI RAATTY 1 cut(s) 88
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 5 cut(s) 155, 297, 575, 582, 951
XmnI GAANNNNTTC 2 cut(s) 387, 448
XspI CTAG 2 cut(s) 39, 311
ZrmI AGTACT 1 cut(s) 750
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.