Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0010113

Physical Location & Seq

Genomic Coordinates Forward (+)

7328052 .. 7337651

Gene Structure 9600 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00391080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGGCGGAGGTGTCGATGTGTAGGAGGAGCAGCCTTAGTCCGGATGAAGAGAGGATTTTGATAAAAGACATTGCTTTGGCTGCCGAAGCCAAATCCAAAGAGGGCGATACCTTCTTCTTGATTACCCAAAGATGGTGGCAACACTGGATTGATTATGTGAACCAAGAACAACAGCCCGATGGCTCAGAGCATTCTGATTTGGCTGATTCAAGTAGCTTCAAGAGGCCTGCTGGTATTGATAATTCCGATTTGATATCCGACGACGCCATTGAGATACAGGATACTTTATTGGAGGGCCGTGACTATGTGTTGCTCCCCCAACAAGTTTGGAACCAGTTGCATACGTGGTATGGAGGTGGGCCAACATTGCCCAGGAGAGTCATTAGTTCAGGTCCTTCTCACACAGAGATGGCTGTTGAAGTCTACCCTCTGCGTCTCCAATTGCTTGTGGTGCCAAAAGGCGGCCACTCGACTATAAGAATAAGCAAGAAAGAAACAATTGGAGAGCTTCACAGAAGAGCTTGTGAAATCTTTGATCTTAGCCCGGAACAAGTATGCATTTGGGATTTCTATGGACGCAAGCAACATGCCCTGATGACCGACATTGATAAAACACTTGATGATGCGAATATACAAATGGACCAGGATGTGGAGCAGACACTGACTACAGTTGTTATCTGTTATGCGCTCTTAAAGTTTGAATCAATGTTTGCTTTTAACAAGATTCTGGTGGAGGTCCTTAGTCCTGACAATGGTACGAGACCGAGTGGATACACAAGTTCTGTTCGTTATGATGGATCTTTAGAAAAGGAAGCTGCCTCAGTTCTTGTAGAGCCTTCTAAGTCAAGCTTGTCGATTGCTGGAGGTTTATCTGCAAGCAAGGGTGCATCAAGAAACTATAACACAGAGCTTGCACAAAGTCAAAGCCTGACTTCAGTTAGAGAATTGGATACTACTTATGGGACAATCGGTGTTAGTACAAGAGGTTCGTCTGGTGGTTTGACTGGGTTGCAGAACCTAGGGAATACTTGTTTTATGAACAGTGCTATACAATGCCTTGTTCATACACCAGAGTTCGCTAGATATTTTCGGGAAGATTATCATCAAGAGATAAACTGGGAAAACACTCTGGGTATGCGCGGTGAGCTAGCTCTAGCATTCGGCGAATTACTGCGGAAGCTTTGGGCACCTGGACGAACACCAGTTGCTCCTCGACCTTTTAAAACAAAACTTGCTCGTTTTGCCCCCCAATTCAGTGGTTACAATCAACATGATTCTCAGGAACTTTTGGCTTTTTTGCTAGATGGTCTTCATGAAGATCTGAATCGTGTGAAACAGAAACCTTATATAAAATCTAAAGATGCTGATGGTCGGCCTGATGAAGAAGTTGCTGATGAATATTGGGCAAACCACATTGCTCGTAATGATTCCATAATCGTCGATGTATGCCAAGGCCAATACAAATCAACTTTGGTTTGCCCCATTTGTAACAAAGTCTCTGTTACATTTGATCCCTTCATGTACCTATCATTGCCCCTGCAGTCCACCACAACTAGAACAATGACAGTAACAGTCTTTACATGCAATGGAAGTGCGTTGCCATCTGTTTACACTGTTACAGTTCCAAAACAGGGACGTTGCCGGGACTTGATCCAGGCACTTAGCAATGCTAGCTCTGTGAAGCACAGTGAGAGGCTTTTGCTTGTTGAGATACAAAACCACATGATTCAGCGATTTCTGGAAGACCCATTAATATTATTATCTACCATCAAAGATGATGATCATCTTGCTGCTTACAAGGTTCAAAAGTTGGCGAAGAACACAAAATATCTTCAGTTGATACACCGTCGCAAGGACCAAGGAACTAGTGATGGCCAGATCACATCAGGATGGCTACCTTATGGAACACCTCTTCTGTCGGCCATCTCATGTGATGATGAAATCACAAGAGGCAACATACAGAACATGGTTCATACAATGCTTTCTCCAATGCTAAGAACTGAGAGTTTGGATCATACTGATATGTCTGGTGCAAGATCTGCAATAGCGGCATCAAACCCCTCTGGAAACTTGCGTGGTGAAGCAATTACTGATTCCGCTCTATCCAATTCAATGAATAATGATATCACCAGTTCTAAGCCAGTGAAATCACTAAAGTTGTCTCTTCAGTTGGTGGATGAAAGTAACGCATGCATTGATCTCTCAGTGGGTGAAGAGAAAGCTATCACATTGCCTTCGGCATCAACATCAATACTAGTATATGCTGATTGGTCACAGAAGCTTTTAGATAAATATCATACCCATTATCTGGAAAACTTGCCAGAAGTTTTTAAGTATGGACCTGTAACAAAGAAAGCCCGCACTGAACCTCTCTCCTTGTACACTTGCCTTGAAGCATTTCTACGTGAAGAACCACTGGTGCCTGAAGATATGTGCAACTCTGGACATCAGCTTTACGAACTGTATGCTTTGACCAATCATTATGGTAGCATGGGTAGTGGGCATTACACTGCACATATCAAGCTTCTTGATGAGAACAGATGGTATAGTTTTGATGACAGTCATATATCACCCATAAATGAAGAAGATGTGAAGTCAGCTGCAGCATATGTACTGTTTTATCGCCGGGTAAAGAGTGAAGATTCCTTTGTTAGCAATGGAGTCCTATCCTGTGGAGGTGACAACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUSP UCH USP7_C2

Protein Analysis

896

Amino Acids

100.06

Weight (kDa)

5.47

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUSP	PF06337	35 - 128	2.6e-20	DUSP domain
Ubiquitin_UBP8	PF25242	147 - 198	3.4e-10	UBP8-like, ubiquitin-like domain
UCH	PF00443	335 - 874	4.4e-59	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40930 AT2G40930
fragaria_vesca	FvH4_1g29170 FvH4_1g29170
malus_domestica	MD17G1207100.v1.1
prunus_persica	Prupe.7G027100_v2.0.a1 Prupe.7G027100_v2.0.a1 Prupe.7G027100_v2.0.a1 Prupe.7G027100_v2.0.a1 Prupe.7G027100_v2.0.a1 Prupe.7G027100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0494551
rosa_laevigata	RLG00000022671
rosa_multiflora	Rmu_sc0000152.1_g000004
rosa_roxburghii	Rroxscaffold_6G00391080
rosa_rugosa	Rorug03G0265600
rosa_samantha	Rh3AG315800 Rh3BG351000 Rh3CG347800 Rh3DG350300
rosa_wichuraiana	Rw3G027570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	451, 1186, 2419
AccB7I	CCANNNNNTGG	2 cut(s)	649, 2308
AccBSI	CCGCTC	1 cut(s)	2099
AccI	GTMKAC	1 cut(s)	423
AccII	CGCG	1 cut(s)	1140
AccIII	TCCGGA	1 cut(s)	40
AciI	CCGC	7 cut(s)	5, 462, 1140, 1174, 2048, 2097, 2359
AclWI	GGATC	4 cut(s)	805, 1505, 1645, 2019
AcoI	YGGCCR	3 cut(s)	463, 1873, 1920
AcuI	CTGAAG	4 cut(s)	918, 1817, 2150, 2445
AcyI	GRCGYC	1 cut(s)	264
AfaI	GTAC	5 cut(s)	757, 979, 1523, 2381, 2613
AfiI	CCNNNNNNNGG	8 cut(s)	40, 132, 461, 649, 752, 1631, 1852, 2308
AgsI	TTSAA	7 cut(s)	210, 220, 419, 701, 1805, 2112, 2393
AhlI	ACTAGT	2 cut(s)	1865, 2254
AjnI	CCWGG	4 cut(s)	371, 642, 1189, 1653
Alw26I	GTCTC	4 cut(s)	440, 754, 1501, 2166
AlwI	GGATC	4 cut(s)	805, 1505, 1645, 2019
AlwNI	CAGNNNCTG	1 cut(s)	661
Aor13HI	TCCGGA	1 cut(s)	40
AoxI	GGCC	8 cut(s)	224, 295, 359, 463, 1373, 1453, 1873, 1920
ApeKI	GCWGC	6 cut(s)	30, 80, 815, 1790, 2600, 2603
AseI	ATTAAT	1 cut(s)	1751
Asp700I	GAANNNNTTC	1 cut(s)	2397
AspA2I	CCTAGG	1 cut(s)	1018
AspLEI	GCGC	2 cut(s)	688, 1140
AspS9I	GGNCC	7 cut(s)	295, 359, 392, 640, 736, 1855, 2339
AsuC2I	CCSGG	3 cut(s)	545, 1643, 2627
AsuHPI	GGTGA	6 cut(s)	1154, 2090, 2119, 2222, 2562, 2690
AsuNHI	GCTAGC	2 cut(s)	1147, 1670
AvaII	GGWCC	5 cut(s)	392, 640, 736, 1855, 2339
AvrII	CCTAGG	1 cut(s)	1018
BaeGI	GKGCMC	1 cut(s)	1189
BaeI	ACNNNNGTAYC	2 cut(s)	763, 796
BalI	TGGCCA	1 cut(s)	1875
BanI	GGYRCC	3 cut(s)	451, 1186, 2419
BarI	GAAGNNNNNNTAC	2 cut(s)	2385, 2417
BbsI	GAAGAC	2 cut(s)	1301, 1749
BbvI	GCAGC	6 cut(s)	42, 67, 802, 1777, 2587, 2615
BceAI	ACGGC	1 cut(s)	282
BcgI	CGANNNNNNTGC	2 cut(s)	2447, 2481
BciT130I	CCWGG	4 cut(s)	373, 644, 1191, 1655
BciVI	GTATCC	3 cut(s)	274, 764, 943
BclI	TGATCA	1 cut(s)	1780
BcnI	CCSGG	3 cut(s)	545, 1643, 2627
BcoDI	GTCTC	4 cut(s)	440, 754, 1501, 2166
BcuI	ACTAGT	2 cut(s)	1865, 2254
BfaI	CTAG	9 cut(s)	1019, 1080, 1148, 1154, 1301, 1554, 1671, 1866, 2255
BfmI	CTRYAG	3 cut(s)	666, 1538, 2601
BfuI	GTATCC	3 cut(s)	274, 764, 943
BglII	AGATCT	2 cut(s)	1318, 2036
BisI	GCNGC	8 cut(s)	31, 81, 463, 816, 1791, 2049, 2601, 2604
BlnI	CCTAGG	1 cut(s)	1018
BlsI	GCNGC	8 cut(s)	32, 82, 464, 817, 1792, 2050, 2602, 2605
Bme1390I	CCNGG	7 cut(s)	373, 545, 644, 1191, 1643, 1655, 2627
Bme18I	GGWCC	5 cut(s)	392, 640, 736, 1855, 2339
BmgT120I	GGNCC	7 cut(s)	295, 359, 392, 640, 736, 1855, 2339
BmiI	GGNNCC	4 cut(s)	332, 453, 1188, 2421
BmrFI	CCNGG	7 cut(s)	373, 545, 644, 1191, 1643, 1655, 2627
BmrI	ACTGGG	2 cut(s)	1014, 1126
BmsI	GCATC	5 cut(s)	613, 896, 1351, 2060, 2249
BmtI	GCTAGC	2 cut(s)	1151, 1674
BmuI	ACTGGG	2 cut(s)	1014, 1126
BpiI	GAAGAC	2 cut(s)	1301, 1749
BpmI	CTGGAG	1 cut(s)	882
BpuMI	CCSGG	3 cut(s)	545, 1643, 2627
BsaAI	YACGTR	2 cut(s)	345, 2405
BsaBI	GATNNNNATC	5 cut(s)	1101, 1323, 1779, 1782, 2292
BsaHI	GRCGYC	1 cut(s)	264
BsaI	GGTCTC	1 cut(s)	754
BsaJI	CCNNGG	4 cut(s)	371, 1018, 1450, 1858
BsaWI	WCCGGW	1 cut(s)	40
Bsc4I	CCNNNNNNNGG	8 cut(s)	40, 132, 461, 649, 752, 1631, 1852, 2308
Bse1I	ACTGG	8 cut(s)	149, 334, 1009, 1121, 1202, 2130, 2141, 2421
Bse3DI	GCAATG	8 cut(s)	69, 365, 1413, 1529, 1591, 1672, 2228, 2662
Bse8I	GATNNNNATC	5 cut(s)	1101, 1323, 1779, 1782, 2292
BseAI	TCCGGA	1 cut(s)	40
BseBI	CCWGG	4 cut(s)	373, 644, 1191, 1655
BseDI	CCNNGG	4 cut(s)	371, 1018, 1450, 1858
BseGI	GGATG	4 cut(s)	49, 652, 1895, 2182
BseJI	GATNNNNATC	5 cut(s)	1101, 1323, 1779, 1782, 2292
BseLI	CCNNNNNNNGG	8 cut(s)	40, 132, 461, 649, 752, 1631, 1852, 2308
BseMI	GCAATG	8 cut(s)	69, 365, 1413, 1529, 1591, 1672, 2228, 2662
BseMII	CTCAG	5 cut(s)	198, 834, 1292, 1992, 2217
BseNI	ACTGG	8 cut(s)	149, 334, 1009, 1121, 1202, 2130, 2141, 2421
BseRI	GAGGAG	2 cut(s)	40, 1200
BseSI	GKGCMC	1 cut(s)	1189
BseXI	GCAGC	6 cut(s)	42, 67, 802, 1777, 2587, 2615
BsgI	GTGCAG	1 cut(s)	2496
Bsh1236I	CGCG	1 cut(s)	1140
BshFI	GGCC	8 cut(s)	226, 297, 361, 465, 1375, 1455, 1875, 1922
BshNI	GGYRCC	3 cut(s)	451, 1186, 2419
BsiSI	CCGG	4 cut(s)	41, 545, 1642, 2626
BslFI	GGGAC	3 cut(s)	976, 1647, 1658
BslI	CCNNNNNNNGG	8 cut(s)	40, 132, 461, 649, 752, 1631, 1852, 2308
BsmAI	GTCTC	4 cut(s)	440, 754, 1501, 2166
BsmBI	CGTCTC	1 cut(s)	440
BsmFI	GGGAC	3 cut(s)	976, 1647, 1658
BsmI	GAATGC	2 cut(s)	190, 1157
BsnI	GGCC	8 cut(s)	226, 297, 361, 465, 1375, 1455, 1875, 1922
Bso31I	GGTCTC	1 cut(s)	754
Bsp1286I	GDGCHC	1 cut(s)	1189
Bsp13I	TCCGGA	1 cut(s)	40
Bsp1407I	TGTACA	1 cut(s)	2379
BspACI	CCGC	7 cut(s)	5, 462, 1140, 1174, 2048, 2097, 2359
BspANI	GGCC	8 cut(s)	226, 297, 361, 465, 1375, 1455, 1875, 1922
BspCNI	CTCAG	5 cut(s)	197, 833, 1291, 1993, 2216
BspEI	TCCGGA	1 cut(s)	40
BspFNI	CGCG	1 cut(s)	1140
BspHI	TCATGA	1 cut(s)	1312
BspLI	GGNNCC	4 cut(s)	332, 453, 1188, 2421
BspMAI	CTGCAG	2 cut(s)	1542, 2605
BspOI	GCTAGC	2 cut(s)	1151, 1674
BspPI	GGATC	4 cut(s)	805, 1505, 1645, 2019
BspQI	GCTCTTC	1 cut(s)	511
BspT107I	GGYRCC	3 cut(s)	451, 1186, 2419
BspTNI	GGTCTC	1 cut(s)	754
BsrBI	CCGCTC	1 cut(s)	2099
BsrDI	GCAATG	8 cut(s)	69, 365, 1413, 1529, 1591, 1672, 2228, 2662
BsrGI	TGTACA	1 cut(s)	2379
BsrI	ACTGG	8 cut(s)	149, 334, 1009, 1121, 1202, 2130, 2141, 2421
BssECI	CCNNGG	4 cut(s)	371, 1018, 1450, 1858
BssNI	GRCGYC	1 cut(s)	264
BssT1I	CCWWGG	3 cut(s)	1018, 1450, 1858
Bst2UI	CCWGG	4 cut(s)	373, 644, 1191, 1655
Bst6I	CTCTTC	5 cut(s)	42, 511, 1917, 2169, 2208
BstACI	GRCGYC	1 cut(s)	264
BstAPI	GCANNNNNTGC	1 cut(s)	2039
BstAUI	TGTACA	1 cut(s)	2379
BstBAI	YACGTR	2 cut(s)	345, 2405
BstC8I	GCNNGC	8 cut(s)	228, 581, 877, 912, 1149, 1672, 2191, 2359
BstF5I	GGATG	4 cut(s)	49, 652, 1895, 2182
BstFNI	CGCG	1 cut(s)	1140
BstHHI	GCGC	2 cut(s)	688, 1140
BstMAI	GTCTC	4 cut(s)	440, 754, 1501, 2166
BstMWI	GCNNNNNNNGC	9 cut(s)	80, 86, 367, 451, 1144, 1241, 1671, 2039, 2048
BstNI	CCWGG	4 cut(s)	373, 644, 1191, 1655
BstNSI	RCATGY	3 cut(s)	590, 1584, 2193
BstSCI	CCNGG	7 cut(s)	371, 543, 642, 1189, 1641, 1653, 2625
BstSFI	CTRYAG	3 cut(s)	666, 1538, 2601
BstSLI	GKGCMC	1 cut(s)	1189
BstUI	CGCG	1 cut(s)	1140
BstV1I	GCAGC	6 cut(s)	42, 67, 802, 1777, 2587, 2615
BstV2I	GAAGAC	2 cut(s)	1301, 1749
BstX2I	RGATCY	3 cut(s)	797, 1318, 2036
BstXI	CCANNNNNNTGG	1 cut(s)	1256
BstYI	RGATCY	3 cut(s)	797, 1318, 2036
BsuI	GTATCC	3 cut(s)	274, 764, 943
BsuRI	GGCC	8 cut(s)	226, 297, 361, 465, 1375, 1455, 1875, 1922
BtsCI	GGATG	4 cut(s)	49, 652, 1895, 2182
BtsI	GCAGTG	1 cut(s)	2508
Cac8I	GCNNGC	8 cut(s)	228, 581, 877, 912, 1149, 1672, 2191, 2359
CaiI	CAGNNNCTG	1 cut(s)	661
CciI	TCATGA	1 cut(s)	1312
CfoI	GCGC	2 cut(s)	688, 1140
Cfr13I	GGNCC	7 cut(s)	295, 359, 392, 640, 736, 1855, 2339
CseI	GACGC	3 cut(s)	272, 422, 585
Csp6I	GTAC	5 cut(s)	756, 978, 1522, 2380, 2612
CspCI	CAANNNNNGTGG	2 cut(s)	116, 151
CviQI	GTAC	5 cut(s)	756, 978, 1522, 2380, 2612
DraI	TTTAAA	1 cut(s)	1222
EaeI	YGGCCR	3 cut(s)	463, 1873, 1920
Eam1104I	CTCTTC	5 cut(s)	42, 511, 1917, 2169, 2208
EarI	CTCTTC	5 cut(s)	42, 511, 1917, 2169, 2208
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	3 cut(s)	1018, 1450, 1858
Eco147I	AGGCCT	1 cut(s)	226
Eco31I	GGTCTC	1 cut(s)	754
Eco32I	GATATC	2 cut(s)	255, 2125
Eco47I	GGWCC	5 cut(s)	392, 640, 736, 1855, 2339
Eco57I	CTGAAG	4 cut(s)	918, 1817, 2150, 2445
EcoO109I	RGGNCCY	2 cut(s)	392, 736
EcoRII	CCWGG	4 cut(s)	371, 642, 1189, 1653
EcoRV	GATATC	2 cut(s)	255, 2125
EcoT14I	CCWWGG	3 cut(s)	1018, 1450, 1858
EcoT22I	ATGCAT	2 cut(s)	560, 2195
ErhI	CCWWGG	3 cut(s)	1018, 1450, 1858
Esp3I	CGTCTC	1 cut(s)	440
FalI	AAGNNNNNCTT	4 cut(s)	833, 865, 916, 948
FaqI	GGGAC	3 cut(s)	976, 1647, 1658
FauI	CCCGC	1 cut(s)	2366
FauNDI	CATATG	1 cut(s)	2608
FbaI	TGATCA	1 cut(s)	1780
FblI	GTMKAC	1 cut(s)	423
Fnu4HI	GCNGC	8 cut(s)	31, 81, 463, 816, 1791, 2049, 2601, 2604
FokI	GGATG	4 cut(s)	56, 659, 1902, 2189
Fsp4HI	GCNGC	8 cut(s)	31, 81, 463, 816, 1791, 2049, 2601, 2604
FspBI	CTAG	9 cut(s)	1019, 1080, 1148, 1154, 1301, 1554, 1671, 1866, 2255
GlaI	GCGC	2 cut(s)	687, 1139
GluI	GCNGC	8 cut(s)	31, 81, 463, 816, 1791, 2049, 2601, 2604
GsuI	CTGGAG	1 cut(s)	882
HaeIII	GGCC	8 cut(s)	226, 297, 361, 465, 1375, 1455, 1875, 1922
HapII	CCGG	4 cut(s)	41, 545, 1642, 2626
HgaI	GACGC	3 cut(s)	272, 422, 585
HhaI	GCGC	2 cut(s)	688, 1140
Hin1I	GRCGYC	1 cut(s)	264
Hin6I	GCGC	2 cut(s)	686, 1138
HinP1I	GCGC	2 cut(s)	686, 1138
HindIII	AAGCTT	4 cut(s)	847, 1178, 2279, 2522
HpaII	CCGG	4 cut(s)	41, 545, 1642, 2626
HphI	GGTGA	6 cut(s)	1154, 2090, 2119, 2222, 2562, 2690
Hpy166II	GTNNAC	5 cut(s)	160, 424, 1545, 1609, 2382
Hpy188I	TCNGA	5 cut(s)	187, 196, 247, 259, 1323
Hpy8I	GTNNAC	5 cut(s)	160, 424, 1545, 1609, 2382
Hpy99I	CGWCG	4 cut(s)	263, 266, 1442, 1851
HpyAV	CCTTC	5 cut(s)	121, 405, 846, 1525, 2244
HpyCH4IV	ACGT	3 cut(s)	344, 1636, 2404
HpyF10VI	GCNNNNNNNGC	9 cut(s)	80, 86, 367, 451, 1144, 1241, 1671, 2039, 2048
HpySE526I	ACGT	3 cut(s)	344, 1636, 2404
Hsp92I	GRCGYC	1 cut(s)	264
HspAI	GCGC	2 cut(s)	686, 1138
Kpn2I	TCCGGA	1 cut(s)	40
Ksp22I	TGATCA	1 cut(s)	1780
LguI	GCTCTTC	1 cut(s)	511
LmnI	GCTCC	4 cut(s)	27, 318, 652, 1213
Lsp1109I	GCAGC	6 cut(s)	42, 67, 802, 1777, 2587, 2615
LweI	GCATC	5 cut(s)	613, 896, 1351, 2060, 2249
MaeI	CTAG	9 cut(s)	1019, 1080, 1148, 1154, 1301, 1554, 1671, 1866, 2255
MaeII	ACGT	3 cut(s)	344, 1636, 2404
MbiI	CCGCTC	1 cut(s)	2099
MfeI	CAATTG	2 cut(s)	440, 498
MflI	RGATCY	3 cut(s)	797, 1318, 2036
MhlI	GDGCHC	1 cut(s)	1189
MlsI	TGGCCA	1 cut(s)	1875
MluCI	AATT	8 cut(s)	241, 440, 498, 944, 1166, 1250, 2085, 2107
MluNI	TGGCCA	1 cut(s)	1875
MlyI	GAGTC	2 cut(s)	387, 2670
MmeI	TCCRAC	1 cut(s)	282
Mox20I	TGGCCA	1 cut(s)	1875
Mph1103I	ATGCAT	2 cut(s)	560, 2195
MroI	TCCGGA	1 cut(s)	40
MroXI	GAANNNNTTC	1 cut(s)	2397
MscI	TGGCCA	1 cut(s)	1875
MseI	TTAA	5 cut(s)	692, 717, 1221, 1751, 2331
MslI	CAYNNNNRTG	2 cut(s)	407, 1888
Msp20I	TGGCCA	1 cut(s)	1875
MspA1I	CMGCKG	1 cut(s)	2600
MspI	CCGG	4 cut(s)	41, 545, 1642, 2626
MspR9I	CCNGG	7 cut(s)	373, 545, 644, 1191, 1643, 1655, 2627
MunI	CAATTG	2 cut(s)	440, 498
Mva1269I	GAATGC	2 cut(s)	190, 1157
MvaI	CCWGG	4 cut(s)	373, 644, 1191, 1655
MvnI	CGCG	1 cut(s)	1140
MwoI	GCNNNNNNNGC	9 cut(s)	80, 86, 367, 451, 1144, 1241, 1671, 2039, 2048
NciI	CCSGG	3 cut(s)	545, 1643, 2627
NdeI	CATATG	1 cut(s)	2608
NheI	GCTAGC	2 cut(s)	1147, 1670
NlaIV	GGNNCC	4 cut(s)	332, 453, 1188, 2421
NmuCI	GTSAC	3 cut(s)	299, 2271, 2678
NsiI	ATGCAT	2 cut(s)	560, 2195
NspI	RCATGY	3 cut(s)	590, 1584, 2193
PaeI	GCATGC	1 cut(s)	2193
PagI	TCATGA	1 cut(s)	1312
PceI	AGGCCT	1 cut(s)	226
PciSI	GCTCTTC	1 cut(s)	511
PctI	GAATGC	2 cut(s)	190, 1157
PdmI	GAANNNNTTC	1 cut(s)	2397
PfeI	GAWTC	9 cut(s)	206, 701, 724, 1274, 1324, 1427, 1726, 2093, 2642
PflMI	CCANNNNNTGG	2 cut(s)	649, 2308
PkrI	GCNGC	8 cut(s)	32, 82, 464, 817, 1792, 2050, 2602, 2605
PleI	GAGTC	2 cut(s)	386, 2669
PpsI	GAGTC	2 cut(s)	386, 2669
Ppu21I	YACGTR	2 cut(s)	345, 2405
PpuMI	RGGWCCY	2 cut(s)	392, 736
PshBI	ATTAAT	1 cut(s)	1751
Psp5II	RGGWCCY	2 cut(s)	392, 736
Psp6I	CCWGG	4 cut(s)	371, 642, 1189, 1653
PspGI	CCWGG	4 cut(s)	371, 642, 1189, 1653
PspN4I	GGNNCC	4 cut(s)	332, 453, 1188, 2421
PspPI	GGNCC	7 cut(s)	295, 359, 392, 640, 736, 1855, 2339
PspPPI	RGGWCCY	2 cut(s)	392, 736
PsrI	GAACNNNNNNTAC	2 cut(s)	970, 1002
PstI	CTGCAG	2 cut(s)	1542, 2605
PstNI	CAGNNNCTG	1 cut(s)	661
PsuI	RGATCY	3 cut(s)	797, 1318, 2036
PvuII	CAGCTG	1 cut(s)	2600
RsaI	GTAC	5 cut(s)	757, 979, 1523, 2381, 2613
RsaNI	GTAC	5 cut(s)	756, 978, 1522, 2380, 2612
RseI	CAYNNNNRTG	2 cut(s)	407, 1888
SapI	GCTCTTC	1 cut(s)	511
SaqAI	TTAA	5 cut(s)	692, 717, 1221, 1751, 2331
SatI	GCNGC	8 cut(s)	31, 81, 463, 816, 1791, 2049, 2601, 2604
Sau96I	GGNCC	7 cut(s)	295, 359, 392, 640, 736, 1855, 2339
SchI	GAGTC	2 cut(s)	387, 2670
ScrFI	CCNGG	7 cut(s)	373, 545, 644, 1191, 1643, 1655, 2627
SduI	GDGCHC	1 cut(s)	1189
SfaNI	GCATC	5 cut(s)	613, 896, 1351, 2060, 2249
SfcI	CTRYAG	3 cut(s)	666, 1538, 2601
SinI	GGWCC	5 cut(s)	392, 640, 736, 1855, 2339
SmiMI	CAYNNNNRTG	2 cut(s)	407, 1888
SpeI	ACTAGT	2 cut(s)	1865, 2254
SphI	GCATGC	1 cut(s)	2193
Sse9I	AATT	8 cut(s)	241, 440, 498, 944, 1166, 1250, 2085, 2107
SseBI	AGGCCT	1 cut(s)	226
SsiI	CCGC	7 cut(s)	5, 462, 1140, 1174, 2048, 2097, 2359
SspI	AATATT	2 cut(s)	1400, 1755
SspMI	CTAG	9 cut(s)	1019, 1080, 1148, 1154, 1301, 1554, 1671, 1866, 2255
StuI	AGGCCT	1 cut(s)	226
StyD4I	CCNGG	7 cut(s)	371, 543, 642, 1189, 1641, 1653, 2625
StyI	CCWWGG	3 cut(s)	1018, 1450, 1858
TaiI	ACGT	3 cut(s)	347, 1639, 2407
TaqI	TCGA	5 cut(s)	14, 470, 854, 1213, 1440
TaqII	GACCGA	2 cut(s)	614, 778
TasI	AATT	8 cut(s)	241, 440, 498, 944, 1166, 1250, 2085, 2107
TatI	WGTACW	3 cut(s)	977, 2379, 2611
TauI	GCSGC	2 cut(s)	465, 2051
TfiI	GAWTC	9 cut(s)	206, 701, 724, 1274, 1324, 1427, 1726, 2093, 2642
Tru1I	TTAA	5 cut(s)	692, 717, 1221, 1751, 2331
Tru9I	TTAA	5 cut(s)	692, 717, 1221, 1751, 2331
TseFI	GTSAC	3 cut(s)	299, 2271, 2678
TseI	GCWGC	6 cut(s)	30, 80, 815, 1790, 2600, 2603
Tsp45I	GTSAC	3 cut(s)	299, 2271, 2678
Van91I	CCANNNNNTGG	2 cut(s)	649, 2308
VpaK11BI	GGWCC	5 cut(s)	392, 640, 736, 1855, 2339
VspI	ATTAAT	1 cut(s)	1751
XceI	RCATGY	3 cut(s)	590, 1584, 2193
XmaJI	CCTAGG	1 cut(s)	1018
XmiI	GTMKAC	1 cut(s)	423
XmnI	GAANNNNTTC	1 cut(s)	2397
XspI	CTAG	9 cut(s)	1019, 1080, 1148, 1154, 1301, 1554, 1671, 1866, 2255
Zsp2I	ATGCAT	2 cut(s)	560, 2195

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.