Rroxscaffold_7G00166060

Transmembrane and coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014677
Physical Location & Seq
Forward (+)
7644378 .. 7649984
5607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00166060.1

Sequence Viewer

Length: 2019 bp
ATGAAAATCTATAGGTTCGCTAAATTGATTCCGTTACTGAATTCTAACCTCTTGCATTTTCGATGCTGTGTGACCATTAAACCCGAAGCAGCGCCGGTTATGGAGGCCAAGGTAGTACTCACACAGACGCAGAGATACGCCGCCGGAGCTCTGTTTGCGCTGGCGCTTCGCCAGACTCAGATCCACCAGACTCGGCCGTTGAATGCTTTGCTCCCTCTTGAGGAGGAGACTATCGGGGATGGTGCCAGTGCTGGCAGGACCATTTCGGTTTCGGATGATCCCGGCATTTGGATCCACGAGAGTTCTGGTTTGCTTTATCCTGTTCTCAGGTTTCTAGGAGTGGAGGACAAATCGTGGAAGGGGCTGAAGGAGACTGCAGGATCTTCTTCACAAGTTAGGCATCATGTAGGGGCGTTCTTGACCTTACTAAAGGAGGGAGCTGATGAGGTGGCTTCTGCTGAGAAAACTGAGAAGGAAGTTGCTCTGACAAAAACTGTTGATACCATGGTTCGTAGTGAGGAAAGCGCCGATGCTCATGCAGTGGATAGGAGTGAGAATTATGAATATGAAGCTAAATGTCGTGAAAAATTTTCCAATACTGAAACGGAAATAGCGTCTGAGGTCTCTGACAAGGCTTATGAGGCCCCAAAGAGGACAGTTTCTGTGATATCCACTGAAATAAAAGATCCAAATGAATCTAAACGCCTTGAGGTGCCTGTTGAAGAGGGAAGGTGGCTCAGTTATGAGAAGAAAATAACAGTTCTTTATGCAATTCTGTCAGCCTGTGTGGCAGACAATAGTGAAAGTGGCGATAAAGCAAGGAACGGTTATGATGCTCGACACCGCGTCACATTGAGGTTGCTGGCAACTTGGCTTGGGGTTGAATGGCTGAAAGTGGAAGTTATGGAGACGATGGTTGCTTGTTCTTTCATGGCTTCGTTAAAAGAGGTTGCTAATAAAGAGAAGCCAGAAGGTGTTGATGGAGAGAAAAAACAAAATGATGATACCTCAGTGGAAAAATGGAAACGGGGAGGACTAATTGGTGCAGCTGCTTTAACAGGAGGAACTTTGATGGCTGTCACGGGTGGTCTAGCTGCTCCTGCAATTGCGCAGGGATTGAGTGCTCTAGCTCCTGTATTGGGTGGCATTGTTCCTGCAATAGGAGCAACTGGCTTCGCTGCTGCAGCAACTGCCACGGGGTCTGTTATTGGTTCTCTTGCTGTTGCTGCATCATTTGGAGGTGCAAAACTGCTGGGGCTGGACTTACGGGATCTAAAATGGCTAGAAGAATTGGCAACATTGAAGAATTTGAGTTCAAAGCAGTTGAAGGAACTTATAACCAAGGATAAACCTGTTCAGCGGCTAGCAGTGGGAATAGTGGTTTCAGGACTTGTTTTTGAGGATGAAGATTTTATGAGGCCTTGGGAAGGCTACAATGACCACTTGGAGAGGTATGCACTGTGGTGGGAGTCTAAGAATTTGGTTACATTGAGCACTGCAATTCAAGACTGGCTTACTTCAAGAATTGCCCTCGAGTTGATGAAAGAAGGTGCCATGCTGACTGTATTAAACACACTAGTAGCAGCGTTCGCTATGCCAGCAACCTTGGTTACTGCATCTGATCTTATAGATAGCAAATGGGCGATTGCAGTTGACAGATCAGATAAAGCTGGGAAGATGCTTGCTGAAGTATTGTTGAAGGGACTGCAAGGCAACAGGCCTGTGACGCTCATAGGATTCTCACTTGGGGGCAGAGTGATTTTTAAATGTCTAGAATGTTTAGCTGAATCAGAAGGGAACAATGCTGGTCTGGTAGAAAGGGTTGTCCTGCTTGGAGCACCAGTGTCAATTGAAGATGAAAATTGGGAAGATGCACGGAAGATGGTGGCAGGGAGATTTGTGAATGCTTTTTCTACAAATGATTGGACACTAGGAGTTGCCTTTCGTGCAAGTTTGCTATCTCAAGGTTTGGCTGGAATTCAAGCTGTCAATGTTCCTGGCATCGAAAATGCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF726

Protein Analysis

672

Amino Acids

72.6

Weight (kDa)

5.42

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF726 PF05277 340 - 670 7.6e-72 Protein of unknown function (DUF726)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g39020 FvH4_2g39020
malus_domestica MD15G1017500.v1.1
prunus_persica Prupe.1G370800_v2.0.a1 Prupe.1G370800_v2.0.a1 Prupe.1G370800_v2.0.a1
pyrus_communis pycom15g01540
rosa_chinensis RchiOBHm_Chr6g0302121
rosa_laevigata RLG00000011193
rosa_multiflora Rmu_ssc0000160.1_g000011
rosa_roxburghii Rroxscaffold_7G00166060
rosa_rugosa Rorug06G0312400
rosa_samantha Rh6AG424800 Rh6BG477600 Rh6CG437700 Rh6DG424800
rosa_wichuraiana Rw6G036800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1337
Acc16I TGCGCA 1 cut(s) 1110
AccB1I GGYRCC 3 cut(s) 242, 712, 1550
AccII CGCG 1 cut(s) 846
AciI CCGC 3 cut(s) 141, 844, 1360
AclWI GGATC 7 cut(s) 175, 272, 286, 299, 388, 680, 1278
AcoI YGGCCR 1 cut(s) 194
AcsI RAATTY 5 cut(s) 40, 587, 1306, 1477, 1977
AcuI CTGAAG 2 cut(s) 386, 1707
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 6 cut(s) 220, 288, 651, 1139, 1160, 1427
AhdI GACNNNNNGTC 1 cut(s) 845
AhlI ACTAGT 1 cut(s) 1576
AjnI CCWGG 1 cut(s) 1996
AleI CACNNNNGTG 1 cut(s) 1462
AluBI AGCT 9 cut(s) 149, 440, 572, 1049, 1094, 1130, 1670, 1784, 1985
AluI AGCT 9 cut(s) 149, 440, 572, 1049, 1094, 1130, 1670, 1784, 1985
Alw21I GWGCWC 4 cut(s) 151, 1126, 1496, 1840
Alw26I GTCTC 4 cut(s) 221, 365, 628, 902
AlwI GGATC 7 cut(s) 175, 272, 286, 299, 388, 680, 1278
AlwNI CAGNNNCTG 2 cut(s) 662, 1190
Ama87I CYCGRG 1 cut(s) 1532
AoxI GGCC 5 cut(s) 105, 194, 642, 1418, 1718
ApeKI GCWGC 9 cut(s) 89, 1046, 1049, 1094, 1178, 1181, 1184, 1226, 1583
ApoI RAATTY 5 cut(s) 40, 587, 1306, 1477, 1977
AspLEI GCGC 5 cut(s) 94, 160, 166, 527, 1111
AspS9I GGNCC 2 cut(s) 258, 643
AsuC2I CCSGG 1 cut(s) 282
AsuNHI GCTAGC 1 cut(s) 1363
AvaI CYCGRG 1 cut(s) 1532
AvaII GGWCC 1 cut(s) 258
BaeI ACNNNNGTAYC 4 cut(s) 492, 525, 996, 1029
BamHI GGATCC 1 cut(s) 291
BanI GGYRCC 3 cut(s) 242, 712, 1550
BanII GRGCYC 1 cut(s) 151
BauI CACGAG 1 cut(s) 296
Bbv12I GWGCWC 4 cut(s) 151, 1126, 1496, 1840
BbvI GCAGC 9 cut(s) 101, 1036, 1058, 1081, 1165, 1168, 1196, 1213, 1595
BccI CCATC 5 cut(s) 233, 907, 974, 1066, 1876
BceAI ACGGC 1 cut(s) 181
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciT130I CCWGG 1 cut(s) 1998
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 4 cut(s) 221, 365, 628, 902
BcuI ACTAGT 1 cut(s) 1576
BfaI CTAG 8 cut(s) 335, 1091, 1127, 1283, 1364, 1577, 1772, 1931
BfmI CTRYAG 3 cut(s) 10, 375, 1182
BfoI RGCGCY 3 cut(s) 95, 167, 528
BglI GCCNNNNNGGC 1 cut(s) 788
BmcAI AGTACT 1 cut(s) 117
Bme1390I CCNGG 2 cut(s) 282, 1998
Bme18I GGWCC 1 cut(s) 258
BmeRI GACNNNNNGTC 1 cut(s) 845
BmeT110I CYCGRG 1 cut(s) 1532
BmgT120I GGNCC 2 cut(s) 258, 643
BmiI GGNNCC 5 cut(s) 244, 293, 645, 714, 1552
BmrFI CCNGG 2 cut(s) 282, 1998
BmsI GCATC 9 cut(s) 53, 409, 520, 823, 1238, 1625, 1668, 1861, 2010
BmtI GCTAGC 1 cut(s) 1367
BpuEI CTTGAG 3 cut(s) 239, 728, 1947
BpuMI CCSGG 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 6 cut(s) 108, 504, 1194, 1341, 1421, 1605
BsaXI ACNNNNNCTCC 2 cut(s) 1827, 1857
Bsc4I CCNNNNNNNGG 6 cut(s) 220, 288, 651, 1139, 1160, 1427
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 4 cut(s) 246, 1174, 1514, 1841
BseBI CCWGG 1 cut(s) 1998
BseDI CCNNGG 6 cut(s) 108, 504, 1194, 1341, 1421, 1605
BseGI GGATG 3 cut(s) 244, 280, 1408
BseLI CCNNNNNNNGG 6 cut(s) 220, 288, 651, 1139, 1160, 1427
BseMII CTCAG 7 cut(s) 191, 340, 450, 459, 609, 751, 1023
BseNI ACTGG 4 cut(s) 246, 1174, 1514, 1841
BseRI GAGGAG 2 cut(s) 236, 239
BseX3I CGGCCG 1 cut(s) 194
BseXI GCAGC 9 cut(s) 101, 1036, 1058, 1081, 1165, 1168, 1196, 1213, 1595
BseYI CCCAGC 2 cut(s) 1252, 1670
BsgI GTGCAG 1 cut(s) 1065
Bsh1236I CGCG 1 cut(s) 846
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 5 cut(s) 107, 196, 644, 1420, 1720
BshNI GGYRCC 3 cut(s) 242, 712, 1550
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 4 cut(s) 151, 1126, 1496, 1840
BsiHKCI CYCGRG 1 cut(s) 1532
BsiSI CCGG 3 cut(s) 95, 144, 282
BslFI GGGAC 1 cut(s) 1716
BslI CCNNNNNNNGG 6 cut(s) 220, 288, 651, 1139, 1160, 1427
BsmAI GTCTC 4 cut(s) 221, 365, 628, 902
BsmBI CGTCTC 1 cut(s) 902
BsmFI GGGAC 1 cut(s) 1716
BsmI GAATGC 2 cut(s) 208, 1909
BsnI GGCC 5 cut(s) 107, 196, 644, 1420, 1720
Bso31I GGTCTC 1 cut(s) 628
BsoBI CYCGRG 1 cut(s) 1532
Bsp1286I GDGCHC 4 cut(s) 151, 1126, 1496, 1840
Bsp143I GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 3 cut(s) 141, 844, 1360
BspANI GGCC 5 cut(s) 107, 196, 644, 1420, 1720
BspCNI CTCAG 7 cut(s) 190, 339, 451, 460, 610, 750, 1022
BspFNI CGCG 1 cut(s) 846
BspLI GGNNCC 5 cut(s) 244, 293, 645, 714, 1552
BspMAI CTGCAG 2 cut(s) 379, 1186
BspOI GCTAGC 1 cut(s) 1367
BspPI GGATC 7 cut(s) 175, 272, 286, 299, 388, 680, 1278
BspT107I GGYRCC 3 cut(s) 242, 712, 1550
BspTNI GGTCTC 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 94
BsrI ACTGG 4 cut(s) 246, 1174, 1514, 1841
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 6 cut(s) 108, 504, 1194, 1341, 1421, 1605
BssMI GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
BssSI CACGAG 1 cut(s) 296
BssT1I CCWWGG 5 cut(s) 108, 504, 1341, 1421, 1605
Bst2BI CACGAG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 1998
Bst4CI ACNGT 6 cut(s) 496, 658, 760, 827, 1461, 1564
Bst6I CTCTTC 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstC8I GCNNGC 6 cut(s) 162, 253, 864, 1365, 1599, 1683
BstDEI CTNAG 8 cut(s) 177, 326, 459, 468, 618, 737, 1009, 1473
BstDSI CCRYGG 2 cut(s) 504, 1194
BstENI CCTNNNNNAGG 2 cut(s) 1158, 1425
BstF5I GGATG 3 cut(s) 244, 280, 1408
BstFNI CGCG 1 cut(s) 846
BstH2I RGCGCY 3 cut(s) 95, 167, 528
BstHHI GCGC 5 cut(s) 94, 160, 166, 527, 1111
BstKTI GATC 8 cut(s) 183, 280, 294, 383, 688, 1273, 1624, 1661
BstMAI GTCTC 4 cut(s) 221, 365, 628, 902
BstMBI GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
BstMCI CGRYCG 1 cut(s) 197
BstNI CCWGG 1 cut(s) 1998
BstSCI CCNGG 2 cut(s) 280, 1996
BstSFI CTRYAG 3 cut(s) 10, 375, 1182
BstUI CGCG 1 cut(s) 846
BstV1I GCAGC 9 cut(s) 101, 1036, 1058, 1081, 1165, 1168, 1196, 1213, 1595
BstX2I RGATCY 5 cut(s) 180, 291, 380, 685, 1270
BstYI RGATCY 5 cut(s) 180, 291, 380, 685, 1270
BstZI CGGCCG 1 cut(s) 194
BsuRI GGCC 5 cut(s) 107, 196, 644, 1420, 1720
BtgI CCRYGG 2 cut(s) 504, 1194
BtsCI GGATG 3 cut(s) 244, 280, 1408
BtsI GCAGTG 3 cut(s) 546, 1374, 1494
BtsIMutI CAGTG 8 cut(s) 253, 546, 672, 1017, 1374, 1457, 1494, 1848
Cac8I GCNNGC 6 cut(s) 162, 253, 864, 1365, 1599, 1683
CaiI CAGNNNCTG 2 cut(s) 662, 1190
CfoI GCGC 5 cut(s) 94, 160, 166, 527, 1111
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 258, 643
CseI GACGC 4 cut(s) 136, 603, 835, 1735
Csp6I GTAC 1 cut(s) 116
CviAII CATG 5 cut(s) 404, 505, 536, 931, 1555
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 8 cut(s) 177, 326, 459, 468, 618, 737, 1009, 1473
DpnI GATC 8 cut(s) 182, 279, 293, 382, 687, 1272, 1623, 1660
DpnII GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
DraI TTTAAA 1 cut(s) 1765
DriI GACNNNNNGTC 1 cut(s) 845
EaeI YGGCCR 1 cut(s) 194
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 717
Eam1105I GACNNNNNGTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 717
Ecl136II GAGCTC 1 cut(s) 149
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 5 cut(s) 108, 504, 1341, 1421, 1605
Eco147I AGGCCT 2 cut(s) 1420, 1720
Eco24I GRGCYC 1 cut(s) 151
Eco31I GGTCTC 1 cut(s) 628
Eco32I GATATC 1 cut(s) 669
Eco47I GGWCC 1 cut(s) 258
Eco52I CGGCCG 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 2 cut(s) 386, 1707
Eco88I CYCGRG 1 cut(s) 1532
EcoICRI GAGCTC 1 cut(s) 149
EcoNI CCTNNNNNAGG 2 cut(s) 1158, 1425
EcoO109I RGGNCCY 1 cut(s) 643
EcoRI GAATTC 2 cut(s) 40, 1977
EcoRII CCWGG 1 cut(s) 1996
EcoRV GATATC 1 cut(s) 669
EcoT14I CCWWGG 5 cut(s) 108, 504, 1341, 1421, 1605
EcoT38I GRGCYC 1 cut(s) 151
ErhI CCWWGG 5 cut(s) 108, 504, 1341, 1421, 1605
Esp3I CGTCTC 1 cut(s) 902
FaeI CATG 5 cut(s) 407, 508, 539, 934, 1558
FalI AAGNNNNNCTT 2 cut(s) 1497, 1529
FaqI GGGAC 1 cut(s) 1716
FatI CATG 5 cut(s) 403, 504, 535, 930, 1554
FokI GGATG 3 cut(s) 251, 287, 1415
FriOI GRGCYC 1 cut(s) 151
FspBI CTAG 8 cut(s) 335, 1091, 1127, 1283, 1364, 1577, 1772, 1931
FspI TGCGCA 1 cut(s) 1110
GlaI GCGC 5 cut(s) 93, 159, 165, 526, 1110
GsaI CCCAGC 2 cut(s) 1256, 1674
HaeII RGCGCY 3 cut(s) 95, 167, 528
HaeIII GGCC 5 cut(s) 107, 196, 644, 1420, 1720
HapII CCGG 3 cut(s) 95, 144, 282
HgaI GACGC 4 cut(s) 136, 603, 835, 1735
HhaI GCGC 5 cut(s) 94, 160, 166, 527, 1111
Hin1II CATG 5 cut(s) 407, 508, 539, 934, 1558
Hin6I GCGC 5 cut(s) 92, 158, 164, 525, 1109
HinP1I GCGC 5 cut(s) 92, 158, 164, 525, 1109
HincII GTYRAC 1 cut(s) 1654
HindII GTYRAC 1 cut(s) 1654
HinfI GANTC 7 cut(s) 28, 175, 190, 695, 1469, 1737, 1787
HpaII CCGG 3 cut(s) 95, 144, 282
Hpy166II GTNNAC 1 cut(s) 1654
Hpy188I TCNGA 8 cut(s) 180, 274, 486, 619, 628, 1621, 1663, 1792
Hpy188III TCNNGA 7 cut(s) 218, 418, 581, 1386, 1505, 1521, 1772
Hpy8I GTNNAC 1 cut(s) 1654
HpyCH4III ACNGT 6 cut(s) 496, 658, 760, 827, 1461, 1564
HpyF3I CTNAG 8 cut(s) 177, 326, 459, 468, 618, 737, 1009, 1473
Hsp92II CATG 5 cut(s) 407, 508, 539, 934, 1558
HspAI GCGC 5 cut(s) 92, 158, 164, 525, 1109
Kzo9I GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
LmnI GCTCC 7 cut(s) 146, 216, 437, 1102, 1135, 1163, 1835
Lsp1109I GCAGC 9 cut(s) 101, 1036, 1058, 1081, 1165, 1168, 1196, 1213, 1595
LweI GCATC 9 cut(s) 53, 409, 520, 823, 1238, 1625, 1668, 1861, 2010
MaeI CTAG 8 cut(s) 335, 1091, 1127, 1283, 1364, 1577, 1772, 1931
MaeIII GTNAC 7 cut(s) 33, 70, 847, 1078, 1483, 1609, 1723
MalI GATC 8 cut(s) 182, 279, 293, 382, 687, 1272, 1623, 1660
MboI GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
MfeI CAATTG 2 cut(s) 1104, 1848
MflI RGATCY 5 cut(s) 180, 291, 380, 685, 1270
MhlI GDGCHC 4 cut(s) 151, 1126, 1496, 1840
MlyI GAGTC 3 cut(s) 169, 184, 1478
MseI TTAA 5 cut(s) 78, 941, 1055, 1568, 1764
MslI CAYNNNNRTG 1 cut(s) 1462
MspA1I CMGCKG 2 cut(s) 1049, 1360
MspI CCGG 3 cut(s) 95, 144, 282
MspR9I CCNGG 2 cut(s) 282, 1998
MunI CAATTG 2 cut(s) 1104, 1848
Mva1269I GAATGC 2 cut(s) 208, 1909
MvaI CCWGG 1 cut(s) 1998
MvnI CGCG 1 cut(s) 846
NciI CCSGG 1 cut(s) 282
NcoI CCATGG 1 cut(s) 504
NdeII GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
NheI GCTAGC 1 cut(s) 1363
NlaIII CATG 5 cut(s) 407, 508, 539, 934, 1558
NlaIV GGNNCC 5 cut(s) 244, 293, 645, 714, 1552
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 4 cut(s) 70, 847, 1078, 1723
NsbI TGCGCA 1 cut(s) 1110
OliI CACNNNNGTG 1 cut(s) 1462
PaeR7I CTCGAG 1 cut(s) 1532
PceI AGGCCT 2 cut(s) 1420, 1720
PcsI WCGNNNNNNNCGW 1 cut(s) 611
PctI GAATGC 2 cut(s) 208, 1909
PfeI GAWTC 4 cut(s) 28, 695, 1737, 1787
PleI GAGTC 3 cut(s) 169, 184, 1477
PpsI GAGTC 3 cut(s) 169, 184, 1477
PsiI TTATAA 1 cut(s) 1337
Psp124BI GAGCTC 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 1996
PspFI CCCAGC 2 cut(s) 1252, 1670
PspGI CCWGG 1 cut(s) 1996
PspN4I GGNNCC 5 cut(s) 244, 293, 645, 714, 1552
PspPI GGNCC 2 cut(s) 258, 643
PspXI VCTCGAGB 1 cut(s) 1532
PstI CTGCAG 2 cut(s) 379, 1186
PstNI CAGNNNCTG 2 cut(s) 662, 1190
PsuI RGATCY 5 cut(s) 180, 291, 380, 685, 1270
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 1462
SacI GAGCTC 1 cut(s) 151
SaqAI TTAA 5 cut(s) 78, 941, 1055, 1568, 1764
Sau3AI GATC 8 cut(s) 180, 277, 291, 380, 685, 1270, 1621, 1658
Sau96I GGNCC 2 cut(s) 258, 643
ScaI AGTACT 1 cut(s) 117
SchI GAGTC 3 cut(s) 169, 184, 1478
ScrFI CCNGG 2 cut(s) 282, 1998
SduI GDGCHC 4 cut(s) 151, 1126, 1496, 1840
SfaNI GCATC 9 cut(s) 53, 409, 520, 823, 1238, 1625, 1668, 1861, 2010
SfcI CTRYAG 3 cut(s) 10, 375, 1182
Sfr274I CTCGAG 1 cut(s) 1532
SinI GGWCC 1 cut(s) 258
SlaI CTCGAG 1 cut(s) 1532
SmiMI CAYNNNNRTG 1 cut(s) 1462
SmlI CTYRAG 4 cut(s) 218, 707, 1532, 1962
SmoI CTYRAG 4 cut(s) 218, 707, 1532, 1962
SpeI ACTAGT 1 cut(s) 1576
SseBI AGGCCT 2 cut(s) 1420, 1720
SsiI CCGC 3 cut(s) 141, 844, 1360
SspMI CTAG 8 cut(s) 335, 1091, 1127, 1283, 1364, 1577, 1772, 1931
SstI GAGCTC 1 cut(s) 151
StuI AGGCCT 2 cut(s) 1420, 1720
StyD4I CCNGG 2 cut(s) 280, 1996
StyI CCWWGG 5 cut(s) 108, 504, 1341, 1421, 1605
TaaI ACNGT 6 cut(s) 496, 658, 760, 827, 1461, 1564
TaqI TCGA 4 cut(s) 61, 838, 1533, 2004
TatI WGTACW 1 cut(s) 115
TauI GCSGC 2 cut(s) 143, 1363
TfiI GAWTC 4 cut(s) 28, 695, 1737, 1787
Tru1I TTAA 5 cut(s) 78, 941, 1055, 1568, 1764
Tru9I TTAA 5 cut(s) 78, 941, 1055, 1568, 1764
TscAI CASTG 8 cut(s) 253, 546, 679, 1017, 1374, 1464, 1501, 1848
TseFI GTSAC 4 cut(s) 70, 847, 1078, 1723
TseI GCWGC 9 cut(s) 89, 1046, 1049, 1094, 1178, 1181, 1184, 1226, 1583
Tsp45I GTSAC 4 cut(s) 70, 847, 1078, 1723
TspDTI ATGAA 8 cut(s) 17, 576, 582, 708, 919, 1419, 1556, 1872
TspGWI ACGGA 3 cut(s) 21, 620, 1891
TspRI CASTG 8 cut(s) 253, 546, 679, 1017, 1374, 1464, 1501, 1848
VpaK11BI GGWCC 1 cut(s) 258
XagI CCTNNNNNAGG 2 cut(s) 1158, 1425
XapI RAATTY 5 cut(s) 40, 587, 1306, 1477, 1977
XbaI TCTAGA 1 cut(s) 1771
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 1532
XspI CTAG 8 cut(s) 335, 1091, 1127, 1283, 1364, 1577, 1772, 1931
ZrmI AGTACT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.