Rorug06G0312400

Transmembrane and coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006482
Physical Location & Seq
Reverse (-)
46385949 .. 46389903
3955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0312400.1

Sequence Viewer

Length: 906 bp
ATGGCGTTTTGGAAACGCCATGGAATTAATTTTAATGGCGTTTTAGAAACGCCATCAATCTGTTTAGGAAAACACGGCACCGCGTTGGAGTCCAGATTTCATGATGTTGAGCTCATCGGCCAAGGTCCTCGGTTCGTTGTCTATGGCGCCAAACTGAAGAATTCCGCCATCAGAGTTGCTGTGAAGGTGATGAGGCCAGAGGTGCTTGCATGTCTCGAGGGTCGGAGTGCCATCTCTTCCGCTCTCCAGTCCGAGCTGCTGAAATCCTTGTCGGCTGCGTACGTGAGGCTTTACCTCTACGACTACGGCAGCGACGGCGCTCCATACTTTTTGTATGAGCGGATGGACGAGAACCTCACCGGAAAAGGAGAGCCCCGAGAGCCGGCTTTGGTCAAGCAGGTGATGCGCGAGCTTTGCCAGGAGCTCGCCGGAATTCACTCCAGCGGCGGAACCCACGGTGCGCTCCACCCAAACAACTTGTTCTTGAAGGATGGACATCTGTTCATCGGAGATTACGGGTTCGAAGGAGCTTTCCCTGTTCCGGCTACCGCAGCAATGCCATATGCTTACTCCGTCATCCGCTACAAGGCTTTGGAGAGAAAGAGGACGGCAAGCGGCGATATTTGGGCAGCGGGCTGTGTTTTCGCCGAGCTGGCCACTGGAAACCCTCTCTTCACTTCAGGGCCAATTTCATCGCAGAAGGAACGGATTTACCGGATTCTTGGAAGGAGGGCCGGCACCGGCGACATCACGGACTCTGACATCGAGTCCTGTAAGATGCGGTTGGCGGCTGCTGTTCCCCAGCTTTCACAGGAAGGGATCGACCTCCTTTATGAGATGCTGCAAGAGCATCCCGTCGACCGGATTGGTGCGGAGGCAGCACTAAAGCATCCTTTTTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF726

Protein Analysis

301

Amino Acids

32.98

Weight (kDa)

7.12

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 33 - 300 1.3e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 1 cut(s) 388
AccB1I GGYRCC 3 cut(s) 77, 146, 737
AccBSI CCGCTC 2 cut(s) 242, 340
AccI GTMKAC 1 cut(s) 858
AccII CGCG 2 cut(s) 83, 408
AclWI GGATC 1 cut(s) 827
AcoI YGGCCR 2 cut(s) 118, 654
AcsI RAATTY 2 cut(s) 160, 432
AcuI CTGAAG 2 cut(s) 176, 663
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 5 cut(s) 382, 541, 586, 861, 899
AgsI TTSAA 1 cut(s) 487
AhdI GACNNNNNGTC 1 cut(s) 766
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 7 cut(s) 112, 256, 412, 424, 530, 652, 805
AluI AGCT 7 cut(s) 112, 256, 412, 424, 530, 652, 805
Alw21I GWGCWC 2 cut(s) 114, 426
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 1 cut(s) 827
Ama87I CYCGRG 2 cut(s) 215, 375
AoxI GGCC 5 cut(s) 118, 194, 654, 683, 732
ApeKI GCWGC 8 cut(s) 256, 275, 309, 551, 629, 791, 841, 878
ApoI RAATTY 2 cut(s) 160, 432
AseI ATTAAT 1 cut(s) 27
AspLEI GCGC 4 cut(s) 149, 320, 408, 463
AspS9I GGNCC 3 cut(s) 125, 683, 732
AsuHPI GGTGA 3 cut(s) 199, 349, 412
AsuII TTCGAA 1 cut(s) 522
AvaI CYCGRG 2 cut(s) 215, 375
AvaII GGWCC 1 cut(s) 125
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 3 cut(s) 77, 146, 737
BanII GRGCYC 3 cut(s) 114, 375, 426
Bbv12I GWGCWC 2 cut(s) 114, 426
BbvI GCAGC 8 cut(s) 243, 262, 321, 563, 641, 778, 828, 890
BccI CCATC 5 cut(s) 61, 176, 239, 337, 485
BceAI ACGGC 4 cut(s) 91, 322, 331, 624
BciT130I CCWGG 1 cut(s) 419
BcoDI GTCTC 1 cut(s) 218
BfoI RGCGCY 2 cut(s) 150, 321
BfuAI ACCTGC 1 cut(s) 388
BglI GCCNNNNNGGC 1 cut(s) 653
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 125
BmeRI GACNNNNNGTC 1 cut(s) 766
BmeT110I CYCGRG 2 cut(s) 215, 375
BmgT120I GGNCC 3 cut(s) 125, 683, 732
BmiI GGNNCC 4 cut(s) 79, 148, 451, 739
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 5 cut(s) 393, 768, 828, 859, 898
BpmI CTGGAG 2 cut(s) 230, 424
Bpu14I TTCGAA 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 283
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 4 cut(s) 19, 121, 128, 454
BsaWI WCCGGW 3 cut(s) 359, 714, 861
Bsc4I CCNNNNNNNGG 5 cut(s) 382, 541, 586, 861, 899
Bse118I RCCGGY 3 cut(s) 382, 734, 740
Bse1I ACTGG 2 cut(s) 247, 664
Bse3DI GCAATG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 4 cut(s) 19, 121, 128, 454
BseGI GGATG 5 cut(s) 348, 496, 576, 850, 889
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 5 cut(s) 382, 541, 586, 861, 899
BseMI GCAATG 1 cut(s) 561
BseNI ACTGG 2 cut(s) 247, 664
BseXI GCAGC 8 cut(s) 243, 262, 321, 563, 641, 778, 828, 890
BseYI CCCAGC 1 cut(s) 801
Bsh1236I CGCG 2 cut(s) 83, 408
Bsh1285I CGRYCG 1 cut(s) 862
BshFI GGCC 5 cut(s) 120, 196, 656, 685, 734
BshNI GGYRCC 3 cut(s) 77, 146, 737
BsiEI CGRYCG 1 cut(s) 862
BsiHKAI GWGCWC 2 cut(s) 114, 426
BsiHKCI CYCGRG 2 cut(s) 215, 375
BsiSI CCGG 8 cut(s) 360, 383, 429, 542, 715, 735, 741, 862
BsiWI CGTACG 1 cut(s) 279
BslI CCNNNNNNNGG 5 cut(s) 382, 541, 586, 861, 899
BsmAI GTCTC 1 cut(s) 218
BsnI GGCC 5 cut(s) 120, 196, 656, 685, 734
BsoBI CYCGRG 2 cut(s) 215, 375
Bsp119I TTCGAA 1 cut(s) 522
Bsp1286I GDGCHC 3 cut(s) 114, 375, 426
Bsp143I GATC 1 cut(s) 819
Bsp19I CCATGG 1 cut(s) 19
BspANI GGCC 5 cut(s) 120, 196, 656, 685, 734
BspFNI CGCG 2 cut(s) 83, 408
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 79, 148, 451, 739
BspMI ACCTGC 1 cut(s) 388
BspPI GGATC 1 cut(s) 827
BspT104I TTCGAA 1 cut(s) 522
BspT107I GGYRCC 3 cut(s) 77, 146, 737
BsrBI CCGCTC 2 cut(s) 242, 340
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 3 cut(s) 382, 734, 740
BsrI ACTGG 2 cut(s) 247, 664
BssAI RCCGGY 3 cut(s) 382, 734, 740
BssECI CCNNGG 4 cut(s) 19, 121, 128, 454
BssMI GATC 1 cut(s) 819
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 19, 121
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 1 cut(s) 458
Bst6I CTCTTC 2 cut(s) 241, 677
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 403
BstBAI YACGTR 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 8 cut(s) 207, 384, 410, 426, 613, 634, 654, 736
BstDSI CCRYGG 2 cut(s) 19, 454
BstF5I GGATG 5 cut(s) 348, 496, 576, 850, 889
BstFNI CGCG 2 cut(s) 83, 408
BstH2I RGCGCY 2 cut(s) 150, 321
BstHHI GCGC 4 cut(s) 149, 320, 408, 463
BstKTI GATC 1 cut(s) 822
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 1 cut(s) 819
BstMCI CGRYCG 1 cut(s) 862
BstMWI GCNNNNNNNGC 9 cut(s) 202, 315, 379, 403, 414, 551, 653, 847, 878
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 417
BstUI CGCG 2 cut(s) 83, 408
BstV1I GCAGC 8 cut(s) 243, 262, 321, 563, 641, 778, 828, 890
BsuRI GGCC 5 cut(s) 120, 196, 656, 685, 734
BtgI CCRYGG 2 cut(s) 19, 454
BtgZI GCGATG 1 cut(s) 678
BtsCI GGATG 5 cut(s) 348, 496, 576, 850, 889
BtsIMutI CAGTG 1 cut(s) 657
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 8 cut(s) 207, 384, 410, 426, 613, 634, 654, 736
CciI TCATGA 1 cut(s) 100
CfoI GCGC 4 cut(s) 149, 320, 408, 463
Cfr10I RCCGGY 3 cut(s) 382, 734, 740
Cfr13I GGNCC 3 cut(s) 125, 683, 732
Csp6I GTAC 1 cut(s) 280
CviAII CATG 3 cut(s) 20, 101, 210
CviQI GTAC 1 cut(s) 280
DinI GGCGCC 1 cut(s) 148
DpnI GATC 1 cut(s) 821
DpnII GATC 1 cut(s) 819
DriI GACNNNNNGTC 1 cut(s) 766
EaeI YGGCCR 2 cut(s) 118, 654
Eam1104I CTCTTC 2 cut(s) 241, 677
Eam1105I GACNNNNNGTC 1 cut(s) 766
EarI CTCTTC 2 cut(s) 241, 677
EciI GGCGGA 2 cut(s) 154, 462
Ecl136II GAGCTC 2 cut(s) 112, 424
Eco130I CCWWGG 2 cut(s) 19, 121
Eco24I GRGCYC 3 cut(s) 114, 375, 426
Eco47I GGWCC 1 cut(s) 125
Eco53kI GAGCTC 2 cut(s) 112, 424
Eco57I CTGAAG 2 cut(s) 176, 663
Eco88I CYCGRG 2 cut(s) 215, 375
EcoICRI GAGCTC 2 cut(s) 112, 424
EcoO109I RGGNCCY 1 cut(s) 125
EcoRI GAATTC 2 cut(s) 160, 432
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 2 cut(s) 19, 121
EcoT38I GRGCYC 3 cut(s) 114, 375, 426
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 19, 121
FaeI CATG 3 cut(s) 23, 104, 213
FaiI YATR 9 cut(s) 21, 102, 144, 211, 325, 336, 562, 564, 834
FatI CATG 3 cut(s) 19, 100, 209
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 562
FblI GTMKAC 1 cut(s) 858
FokI GGATG 5 cut(s) 355, 503, 563, 837, 876
FriOI GRGCYC 3 cut(s) 114, 375, 426
GlaI GCGC 4 cut(s) 148, 319, 407, 462
GsaI CCCAGC 1 cut(s) 805
GsuI CTGGAG 2 cut(s) 230, 424
HaeII RGCGCY 2 cut(s) 150, 321
HaeIII GGCC 5 cut(s) 120, 196, 656, 685, 734
HapII CCGG 8 cut(s) 360, 383, 429, 542, 715, 735, 741, 862
HhaI GCGC 4 cut(s) 149, 320, 408, 463
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 23, 104, 213
Hin6I GCGC 4 cut(s) 147, 318, 406, 461
HinP1I GCGC 4 cut(s) 147, 318, 406, 461
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 4 cut(s) 89, 718, 755, 767
HpaII CCGG 8 cut(s) 360, 383, 429, 542, 715, 735, 741, 862
HphI GGTGA 3 cut(s) 199, 349, 412
Hpy166II GTNNAC 1 cut(s) 859
Hpy188I TCNGA 5 cut(s) 173, 225, 253, 509, 760
Hpy188III TCNNGA 4 cut(s) 93, 101, 215, 484
Hpy8I GTNNAC 1 cut(s) 859
Hpy99I CGWCG 2 cut(s) 317, 860
HpyAV CCTTC 6 cut(s) 178, 481, 518, 694, 720, 809
HpyCH4III ACNGT 1 cut(s) 458
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 2 cut(s) 209, 844
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 315, 379, 403, 414, 551, 653, 847, 878
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 23, 104, 213
HspAI GCGC 4 cut(s) 147, 318, 406, 461
KasI GGCGCC 1 cut(s) 146
KroI GCCGGC 2 cut(s) 382, 734
KroNI GCCGGC 2 cut(s) 384, 736
Kzo9I GATC 1 cut(s) 819
LmnI GCTCC 4 cut(s) 325, 421, 468, 527
Lsp1109I GCAGC 8 cut(s) 243, 262, 321, 563, 641, 778, 828, 890
LweI GCATC 5 cut(s) 393, 768, 828, 859, 898
MaeII ACGT 1 cut(s) 282
MalI GATC 1 cut(s) 821
MbiI CCGCTC 2 cut(s) 242, 340
MboI GATC 1 cut(s) 819
MboII GAAGA 3 cut(s) 169, 228, 664
MhlI GDGCHC 3 cut(s) 114, 375, 426
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 5 cut(s) 24, 28, 160, 432, 687
MluNI TGGCCA 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 98, 749, 776
MmeI TCCRAC 2 cut(s) 66, 203
Mox20I TGGCCA 1 cut(s) 656
MroNI GCCGGC 2 cut(s) 382, 734
MscI TGGCCA 1 cut(s) 656
MseI TTAA 3 cut(s) 27, 33, 904
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 444, 632
MspI CCGG 8 cut(s) 360, 383, 429, 542, 715, 735, 741, 862
MspR9I CCNGG 1 cut(s) 419
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 2 cut(s) 83, 408
MwoI GCNNNNNNNGC 9 cut(s) 202, 315, 379, 403, 414, 551, 653, 847, 878
NaeI GCCGGC 2 cut(s) 384, 736
NarI GGCGCC 1 cut(s) 147
NcoI CCATGG 1 cut(s) 19
NdeI CATATG 1 cut(s) 562
NdeII GATC 1 cut(s) 819
NgoMIV GCCGGC 2 cut(s) 382, 734
NlaIII CATG 3 cut(s) 23, 104, 213
NlaIV GGNNCC 4 cut(s) 79, 148, 451, 739
NmeAIII GCCGAG 1 cut(s) 673
NspI RCATGY 1 cut(s) 213
NspV TTCGAA 1 cut(s) 522
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 100
PaqCI CACCTGC 1 cut(s) 388
PdiI GCCGGC 2 cut(s) 384, 736
PfeI GAWTC 1 cut(s) 718
Pfl23II CGTACG 1 cut(s) 279
PleI GAGTC 3 cut(s) 97, 749, 775
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 3 cut(s) 97, 749, 775
Ppu21I YACGTR 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 27
Psp124BI GAGCTC 2 cut(s) 114, 426
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 1 cut(s) 417
PspLI CGTACG 1 cut(s) 279
PspN4I GGNNCC 4 cut(s) 79, 148, 451, 739
PspPI GGNCC 3 cut(s) 125, 683, 732
PspPPI RGGWCCY 1 cut(s) 125
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 2 cut(s) 114, 426
SalI GTCGAC 1 cut(s) 857
SaqAI TTAA 3 cut(s) 27, 33, 904
Sau3AI GATC 1 cut(s) 819
Sau96I GGNCC 3 cut(s) 125, 683, 732
SchI GAGTC 3 cut(s) 98, 749, 776
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 3 cut(s) 114, 375, 426
SfaNI GCATC 5 cut(s) 393, 768, 828, 859, 898
SfoI GGCGCC 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 740
SinI GGWCC 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 5 cut(s) 24, 28, 160, 432, 687
SspDI GGCGCC 1 cut(s) 146
SstI GAGCTC 2 cut(s) 114, 426
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 2 cut(s) 19, 121
TaaI ACNGT 1 cut(s) 458
TaiI ACGT 1 cut(s) 285
TaqI TCGA 5 cut(s) 216, 522, 765, 822, 858
TasI AATT 5 cut(s) 24, 28, 160, 432, 687
TauI GCSGC 3 cut(s) 447, 618, 791
TfiI GAWTC 1 cut(s) 718
Tru1I TTAA 3 cut(s) 27, 33, 904
Tru9I TTAA 3 cut(s) 27, 33, 904
TscAI CASTG 1 cut(s) 664
TseI GCWGC 8 cut(s) 256, 275, 309, 551, 629, 791, 841, 878
TspDTI ATGAA 3 cut(s) 89, 493, 681
TspGWI ACGGA 3 cut(s) 562, 721, 767
TspRI CASTG 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 125
VspI ATTAAT 1 cut(s) 27
XapI RAATTY 2 cut(s) 160, 432
XceI RCATGY 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.