Rroxscaffold_7G00168100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0019823
Physical Location & Seq
Reverse (-)
9013499 .. 9016272
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00168100.1

Sequence Viewer

Length: 741 bp
ATGGCCAATCATAGGCCACCTCCACCTTCTAGGCGGCGGATACCGGCTACTCTACCGCATATTGGGCTCAATGGCCGACAAATTCAGCCCATCTTTCAACATCGGGCTCGGCAGCCCCTGTGCCTTTGCGAAGCGCGTTGGGAAGTAGCCAACTGTTTCTTCACCTACACATCCGACCACGGGCTACAACTACGCCGTGTTTGGCTTGGCACCCTACGGCCCTTCGGGCGTGAGAGAGGAAGATTAAACACTCGAACTCCTCTCGAACCGCCTCTTGAGATGCTCAAACATGTCCGCACTTCTAAAGTTGAAAACTTGAAATGGGAATACGAGAGTTGCACGGTGTGTGGGTCGAAAAAGAGTCAAGCGCTCCGTGCTTGTGGAAATAAAGCATTGGTTGGAGGAGTCGATCAATGTGGTGGCTATGAAGAAGACCGCCAAGGACTTGGATATCAAATACTTGGTGGGAGGCTGGAGGAGCATCGCCAGAGGAGACTAAAATCTAACTGTGATAAGGTCAAGGCCAAAGGTGTGGAGGAGGCTAGGAGGACTTCGTTACTGTCATGTTGTCTCTTCAGGAACAAGGAGAGCTCTCCAACTTTCAGCATGACAGCATCAAGTCTACCTGTCTGGTACTCTGACGTGGGCCATCTCGTTATTCTTGAACACTGGGCACATGTTAAAGAAGGGATCCAGAAGGCACAGGAAGAGTTAGACCTCCAGGTAGGTACAGACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

28.05

Weight (kDa)

9.5

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019823)

Species Orthologous Gene IDs
pyrus_communis pycom08g03100
rosa_chinensis RchiOBHm_Chr5g0058881 RchiOBHm_Chr6g0300081
rosa_laevigata RLG00000011366 RLG00000011368
rosa_roxburghii Rroxscaffold_7G00168100
rosa_rugosa Rorug06G0293800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 622
AccII CGCG 1 cut(s) 136
AciI CCGC 6 cut(s) 34, 37, 56, 269, 295, 436
AclWI GGATC 2 cut(s) 685, 698
AcoI YGGCCR 2 cut(s) 3, 73
AcsI RAATTY 1 cut(s) 81
AcuI CTGAAG 1 cut(s) 559
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 2 cut(s) 635, 730
AfeI AGCGCT 1 cut(s) 369
AfiI CCNNNNNNNGG 3 cut(s) 12, 62, 180
AflIII ACRYGT 2 cut(s) 289, 676
AgeI ACCGGT 1 cut(s) 735
AgsI TTSAA 4 cut(s) 98, 311, 319, 665
AjiI CACGTC 1 cut(s) 643
AjnI CCWGG 1 cut(s) 720
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 1 cut(s) 591
AluI AGCT 1 cut(s) 591
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 2 cut(s) 487, 575
AlwI GGATC 2 cut(s) 685, 698
AlwNI CAGNNNCTG 1 cut(s) 118
Aor51HI AGCGCT 1 cut(s) 369
AoxI GGCC 6 cut(s) 3, 14, 73, 218, 522, 646
ApeKI GCWGC 1 cut(s) 112
ApoI RAATTY 1 cut(s) 81
AsiGI ACCGGT 1 cut(s) 735
AspLEI GCGC 2 cut(s) 136, 370
AspS9I GGNCC 2 cut(s) 219, 646
AsuHPI GGTGA 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 676
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 690
BanI GGYRCC 1 cut(s) 209
BanII GRGCYC 3 cut(s) 69, 109, 593
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 1 cut(s) 124
BccI CCATC 2 cut(s) 98, 657
BceAI ACGGC 2 cut(s) 180, 233
BciT130I CCWGG 1 cut(s) 722
BciVI GTATCC 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 487, 575
BfaI CTAG 2 cut(s) 30, 543
BfoI RGCGCY 1 cut(s) 371
BfuI GTATCC 1 cut(s) 33
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 2 cut(s) 35, 113
BlsI GCNGC 2 cut(s) 36, 114
Bme1390I CCNGG 1 cut(s) 722
BmgBI CACGTC 1 cut(s) 643
BmgT120I GGNCC 2 cut(s) 219, 646
BmiI GGNNCC 2 cut(s) 211, 692
BmrFI CCNGG 1 cut(s) 722
BmrI ACTGGG 1 cut(s) 679
BmsI GCATC 3 cut(s) 270, 490, 623
BmuI ACTGGG 1 cut(s) 679
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 2 cut(s) 494, 704
BpuEI CTTGAG 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 178, 439
BsaWI WCCGGW 1 cut(s) 735
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 62, 180
Bse118I RCCGGY 2 cut(s) 43, 735
Bse1I ACTGG 1 cut(s) 674
BseBI CCWGG 1 cut(s) 722
BseDI CCNNGG 2 cut(s) 178, 439
BseGI GGATG 1 cut(s) 170
BseLI CCNNNNNNNGG 3 cut(s) 12, 62, 180
BseNI ACTGG 1 cut(s) 674
BseRI GAGGAG 5 cut(s) 249, 417, 491, 505, 551
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 136
BshFI GGCC 6 cut(s) 5, 16, 75, 220, 524, 648
BshNI GGYRCC 1 cut(s) 209
BshTI ACCGGT 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 593
BsiSI CCGG 2 cut(s) 44, 736
BslI CCNNNNNNNGG 3 cut(s) 12, 62, 180
BsmAI GTCTC 2 cut(s) 487, 575
BsnI GGCC 6 cut(s) 5, 16, 75, 220, 524, 648
Bsp1286I GDGCHC 4 cut(s) 69, 109, 593, 676
Bsp143I GATC 2 cut(s) 409, 690
BspACI CCGC 6 cut(s) 34, 37, 56, 269, 295, 436
BspANI GGCC 6 cut(s) 5, 16, 75, 220, 524, 648
BspFNI CGCG 1 cut(s) 136
BspLI GGNNCC 2 cut(s) 211, 692
BspPI GGATC 2 cut(s) 685, 698
BspT107I GGYRCC 1 cut(s) 209
BsrFI RCCGGY 2 cut(s) 43, 735
BsrI ACTGG 1 cut(s) 674
BssAI RCCGGY 2 cut(s) 43, 735
BssECI CCNNGG 2 cut(s) 178, 439
BssMI GATC 2 cut(s) 409, 690
BssT1I CCWWGG 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 722
Bst4CI ACNGT 4 cut(s) 155, 343, 509, 561
Bst6I CTCTTC 2 cut(s) 578, 702
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 136
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 2 cut(s) 136, 370
BstKTI GATC 2 cut(s) 412, 693
BstMAI GTCTC 2 cut(s) 487, 575
BstMBI GATC 2 cut(s) 409, 690
BstMWI GCNNNNNNNGC 4 cut(s) 64, 226, 374, 478
BstNI CCWGG 1 cut(s) 722
BstNSI RCATGY 2 cut(s) 293, 680
BstSCI CCNGG 1 cut(s) 720
BstSLI GKGCMC 1 cut(s) 676
BstUI CGCG 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 690
BstXI CCANNNNNNTGG 2 cut(s) 446, 532
BstYI RGATCY 1 cut(s) 690
BsuI GTATCC 1 cut(s) 33
BsuRI GGCC 6 cut(s) 5, 16, 75, 220, 524, 648
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 467
BtrI CACGTC 1 cut(s) 643
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 1 cut(s) 667
CaiI CAGNNNCTG 1 cut(s) 118
CfoI GCGC 2 cut(s) 136, 370
Cfr10I RCCGGY 2 cut(s) 43, 735
Cfr13I GGNCC 2 cut(s) 219, 646
Csp6I GTAC 2 cut(s) 634, 729
CspAI ACCGGT 1 cut(s) 735
CviAII CATG 4 cut(s) 290, 564, 607, 677
CviQI GTAC 2 cut(s) 634, 729
DpnI GATC 2 cut(s) 411, 692
DpnII GATC 2 cut(s) 409, 690
DraIII CACNNNGTG 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 3, 73
Eam1104I CTCTTC 2 cut(s) 578, 702
EarI CTCTTC 2 cut(s) 578, 702
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 3 cut(s) 69, 109, 593
Eco32I GATATC 1 cut(s) 452
Eco47III AGCGCT 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 591
Eco57I CTGAAG 1 cut(s) 559
EcoICRI GAGCTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 720
EcoRV GATATC 1 cut(s) 452
EcoT14I CCWWGG 1 cut(s) 439
EcoT38I GRGCYC 3 cut(s) 69, 109, 593
ErhI CCWWGG 1 cut(s) 439
FaeI CATG 4 cut(s) 293, 567, 610, 680
FaiI YATR 7 cut(s) 12, 60, 291, 426, 565, 608, 678
FatI CATG 4 cut(s) 289, 563, 606, 676
FblI GTMKAC 1 cut(s) 622
Fnu4HI GCNGC 2 cut(s) 35, 113
FokI GGATG 1 cut(s) 157
FriOI GRGCYC 3 cut(s) 69, 109, 593
Fsp4HI GCNGC 2 cut(s) 35, 113
FspBI CTAG 2 cut(s) 30, 543
GlaI GCGC 2 cut(s) 135, 369
GluI GCNGC 2 cut(s) 35, 113
GsuI CTGGAG 2 cut(s) 494, 704
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 6 cut(s) 5, 16, 75, 220, 524, 648
HapII CCGG 2 cut(s) 44, 736
HhaI GCGC 2 cut(s) 136, 370
Hin1II CATG 4 cut(s) 293, 567, 610, 680
Hin6I GCGC 2 cut(s) 134, 368
HinP1I GCGC 2 cut(s) 134, 368
HinfI GANTC 2 cut(s) 361, 405
HpaII CCGG 2 cut(s) 44, 736
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 623
Hpy188I TCNGA 2 cut(s) 175, 640
Hpy188III TCNNGA 5 cut(s) 263, 275, 577, 662, 694
Hpy8I GTNNAC 1 cut(s) 623
HpyAV CCTTC 4 cut(s) 36, 232, 680, 691
HpyCH4III ACNGT 4 cut(s) 155, 343, 509, 561
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 226, 374, 478
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 4 cut(s) 293, 567, 610, 680
HspAI GCGC 2 cut(s) 134, 368
Kzo9I GATC 2 cut(s) 409, 690
LmnI GCTCC 2 cut(s) 375, 478
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 3 cut(s) 270, 490, 623
MaeI CTAG 2 cut(s) 30, 543
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 1 cut(s) 555
MalI GATC 2 cut(s) 411, 692
MboI GATC 2 cut(s) 409, 690
MboII GAAGA 6 cut(s) 151, 252, 440, 443, 565, 719
MflI RGATCY 1 cut(s) 690
MhlI GDGCHC 4 cut(s) 69, 109, 593, 676
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 81
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 370, 414
MmeI TCCRAC 3 cut(s) 198, 379, 620
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 245, 681
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 44, 736
MspR9I CCNGG 1 cut(s) 722
MvaI CCWGG 1 cut(s) 722
MvnI CGCG 1 cut(s) 136
MwoI GCNNNNNNNGC 4 cut(s) 64, 226, 374, 478
NdeII GATC 2 cut(s) 409, 690
NlaIII CATG 4 cut(s) 293, 567, 610, 680
NlaIV GGNNCC 2 cut(s) 211, 692
NmeAIII GCCGAG 1 cut(s) 88
NspI RCATGY 2 cut(s) 293, 680
PciI ACATGT 2 cut(s) 289, 676
PinAI ACCGGT 1 cut(s) 735
PkrI GCNGC 2 cut(s) 36, 114
PleI GAGTC 2 cut(s) 369, 413
PpsI GAGTC 2 cut(s) 369, 413
PscI ACATGT 2 cut(s) 289, 676
Psp124BI GAGCTC 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 720
PspGI CCWGG 1 cut(s) 720
PspN4I GGNNCC 2 cut(s) 211, 692
PspPI GGNCC 2 cut(s) 219, 646
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 690
RsaI GTAC 2 cut(s) 635, 730
RsaNI GTAC 2 cut(s) 634, 729
SacI GAGCTC 1 cut(s) 593
SaqAI TTAA 2 cut(s) 245, 681
SatI GCNGC 2 cut(s) 35, 113
Sau3AI GATC 2 cut(s) 409, 690
Sau96I GGNCC 2 cut(s) 219, 646
SchI GAGTC 2 cut(s) 370, 414
ScrFI CCNGG 1 cut(s) 722
SduI GDGCHC 4 cut(s) 69, 109, 593, 676
SfaNI GCATC 3 cut(s) 270, 490, 623
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 1 cut(s) 81
SsiI CCGC 6 cut(s) 34, 37, 56, 269, 295, 436
SspMI CTAG 2 cut(s) 30, 543
SstI GAGCTC 1 cut(s) 593
StyD4I CCNGG 1 cut(s) 720
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 4 cut(s) 155, 343, 509, 561
TaiI ACGT 1 cut(s) 645
TaqI TCGA 4 cut(s) 253, 264, 353, 408
TasI AATT 1 cut(s) 81
TauI GCSGC 1 cut(s) 37
Tru1I TTAA 2 cut(s) 245, 681
Tru9I TTAA 2 cut(s) 245, 681
TscAI CASTG 1 cut(s) 674
TseI GCWGC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 441
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 674
XapI RAATTY 1 cut(s) 81
XceI RCATGY 2 cut(s) 293, 680
XmiI GTMKAC 1 cut(s) 622
XspI CTAG 2 cut(s) 30, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.