Rroxscaffold_7G00212810

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018369
Physical Location & Seq
Reverse (-)
63326745 .. 63330207
3463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212810.1

Sequence Viewer

Length: 1428 bp
ATGGGTTCCAAAGCAAGCTCACTATGCTTGCATTTCTTGCTTCTGCTGCTTCTCTCTGGCACTGTGCTCTCGGTTCATTGGCCCGTAAAATCTCTGCCTGGCTACTCCGGTGATTTGCCCTTCAACCTTGAAACTGGGTATATTAGTGTGGATGAAATCGAGTTCTTCTACTACTTTGTCGAGTCTGAAGGGAACCCTGGAGCTGACCCAGTTTTGCTTTACCTCAATGGAGGCCCTGGATGTTCGGGTTTAAACGGATTCTTCTACCAAATTGGCCCGTTGAAGTTCAACATTACAGATTACACCGGGGGCTTACCGACATTGATGTACGAACCAGAAACATGGACAAAGACGGCCAGTATAATATTTTTGGATGCACCTGTTGGTGCCGGTTTCTCATACGCCAACGACAGTGCCGCTTGGACCACAACAGATACACAAGCAGCAAGCCAGTACAATGACTTTTTGAGAAGTTGGATAGATGAACACCCGGATTTTAGTACAAATCCAGTCTACATTGGTAGTGATTCATATGCTGGAATGAATCTTCCCATCCTTGCTCAAGATATAATCAACAGCAATGAAGCTGGAGTCGAGCCAACTATATACCTCAAAGGAATGGTACTTAGCTGTCCGCATACAAATACTGATATTGAAACAAATGCCAAAGTAATTTTTGCCCATAGGATGGCTCTAATATCAGATTCAATGTACGAGTCTGCCAAAAACAGTTGCAATGGGGATTATGTGGATACAACCACTGCAGCATGCACAGAAAGTCTCAGTGCTATAACCCAGTGCATTGCTGAAATAAGTGTAACCAGTACCTTGGATCCCGATTGCACTTTCTTGTCCCCGAAAGAAAAGGAAGAAGAGGAAGAAAAGGAAGAAAAAGCCGCCAGAAGATCTCTCAGACAAAACCCCATCAGGAGTTCATTACGCCTACCATCATCAATTACAAACCAAAAAGATTTTTATTGCCATAATTTCCCTTATTTGCTTTCTGATGTATGGGGAAATGATGAAGGCGTCCAAGAGGCTCTTCATGTTCGACCGGGAACTGTCAAAATATTTTTTAGGTGCAACGTTAGTCTCTCTTATACTGTAGACACCAATGATGCCGTCGCTTATCACAATAATCTGACCGACACAGGCCTGCAATTACTGGTTTTCAGTGGTGACCATGACATGGTTATTCCACATAATGGTATTGAGTACTGGATAACCATATTAGACCTCACTCTCGACACCGATTGGCGTCCTTGGTTTGTTGATGGTCAAGTCGCGGGGTACACAAGGAGGTATACGAACGATGGATATCGCTTGACATATGCAACCATTAAGGGAGCTGGTCACTCACCTCACGAGTACAAACGCAAGGAGGTTTTTGACATGTTCGATAGGTGGATCCATTATTATCCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

475

Amino Acids

53.14

Weight (kDa)

4.67

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 33 - 471 1.2e-103 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018369)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03522
rosa_chinensis RchiOBHm_Chr6g0248941
rosa_laevigata RLG00000015107 RLG00000015108
rosa_multiflora Rmu_sc0003692.1_g000031
rosa_roxburghii Rroxscaffold_7G00212810
rosa_rugosa Rorug05G0534500 Rorug05G0534600
rosa_samantha Rh6AG051800
rosa_wichuraiana Rw6G004600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 3 cut(s) 688, 1189, 1205
AccI GTMKAC 3 cut(s) 513, 1107, 1304
AccII CGCG 1 cut(s) 1286
AciI CCGC 4 cut(s) 417, 635, 897, 1286
AclI AACGTT 1 cut(s) 1086
AclWI GGATC 4 cut(s) 827, 840, 1402, 1415
AcoI YGGCCR 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 207
AcyI GRCGYC 2 cut(s) 1029, 1258
AfaI GTAC 9 cut(s) 329, 455, 502, 624, 713, 826, 1217, 1292, 1370
AfiI CCNNNNNNNGG 3 cut(s) 688, 1189, 1205
AflIII ACRYGT 1 cut(s) 1392
AgsI TTSAA 6 cut(s) 124, 131, 283, 289, 656, 708
AjnI CCWGG 3 cut(s) 97, 196, 235
AjuI GAANNNNNNNTTGG 2 cut(s) 1026, 1058
AluBI AGCT 5 cut(s) 18, 203, 587, 630, 1349
AluI AGCT 5 cut(s) 18, 203, 587, 630, 1349
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 785, 1097
AlwI GGATC 4 cut(s) 827, 840, 1402, 1415
AoxI GGCC 5 cut(s) 80, 232, 274, 354, 1153
ApeKI GCWGC 3 cut(s) 46, 443, 764
AspS9I GGNCC 4 cut(s) 81, 233, 275, 423
AsuC2I CCSGG 3 cut(s) 307, 491, 1056
AsuHPI GGTGA 3 cut(s) 122, 1190, 1350
AvaII GGWCC 1 cut(s) 423
BamHI GGATCC 2 cut(s) 832, 1407
BanI GGYRCC 1 cut(s) 386
BauI CACGAG 1 cut(s) 1364
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 3 cut(s) 33, 455, 776
BccI CCATC 6 cut(s) 560, 682, 932, 955, 1268, 1307
BceAI ACGGC 2 cut(s) 369, 1106
BciT130I CCWGG 3 cut(s) 99, 198, 237
BciVI GTATCC 1 cut(s) 745
BcnI CCSGG 3 cut(s) 307, 491, 1056
BcoDI GTCTC 2 cut(s) 785, 1097
BfmI CTRYAG 2 cut(s) 762, 1104
BfuI GTATCC 1 cut(s) 745
BglII AGATCT 1 cut(s) 905
BisI GCNGC 5 cut(s) 47, 417, 444, 765, 897
BlsI GCNGC 5 cut(s) 48, 418, 445, 766, 898
BmcAI AGTACT 1 cut(s) 1217
Bme1390I CCNGG 6 cut(s) 99, 198, 237, 307, 491, 1056
Bme18I GGWCC 1 cut(s) 423
BmgT120I GGNCC 4 cut(s) 81, 233, 275, 423
BmiI GGNNCC 5 cut(s) 7, 194, 388, 834, 1409
BmrFI CCNGG 6 cut(s) 99, 198, 237, 307, 491, 1056
BmrI ACTGGG 3 cut(s) 144, 203, 790
BmsI GCATC 2 cut(s) 364, 1108
BmuI ACTGGG 3 cut(s) 144, 203, 790
BpmI CTGGAG 2 cut(s) 219, 609
BpuEI CTTGAG 1 cut(s) 546
BpuMI CCSGG 3 cut(s) 307, 491, 1056
BsaBI GATNNNNATC 1 cut(s) 1317
BsaHI GRCGYC 2 cut(s) 1029, 1258
BsaJI CCNNGG 5 cut(s) 196, 235, 306, 828, 1262
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 3 cut(s) 688, 1189, 1205
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 9 cut(s) 139, 209, 357, 451, 509, 796, 822, 1170, 1223
Bse3DI GCAATG 3 cut(s) 586, 742, 801
Bse8I GATNNNNATC 1 cut(s) 1317
BseBI CCWGG 3 cut(s) 99, 198, 237
BseDI CCNNGG 5 cut(s) 196, 235, 306, 828, 1262
BseGI GGATG 5 cut(s) 157, 245, 379, 552, 693
BseJI GATNNNNATC 1 cut(s) 1317
BseLI CCNNNNNNNGG 3 cut(s) 688, 1189, 1205
BseMI GCAATG 3 cut(s) 586, 742, 801
BseMII CTCAG 2 cut(s) 796, 925
BseNI ACTGG 9 cut(s) 139, 209, 357, 451, 509, 796, 822, 1170, 1223
BseXI GCAGC 3 cut(s) 33, 455, 776
Bsh1236I CGCG 1 cut(s) 1286
Bsh1285I CGRYCG 1 cut(s) 1055
BshFI GGCC 5 cut(s) 82, 234, 276, 356, 1155
BshNI GGYRCC 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 1055
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 5 cut(s) 108, 306, 390, 491, 1055
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 3 cut(s) 688, 1189, 1205
BsmAI GTCTC 2 cut(s) 785, 1097
BsmFI GGGAC 1 cut(s) 838
BsnI GGCC 5 cut(s) 82, 234, 276, 356, 1155
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 3 cut(s) 832, 905, 1407
BspACI CCGC 4 cut(s) 417, 635, 897, 1286
BspANI GGCC 5 cut(s) 82, 234, 276, 356, 1155
BspCNI CTCAG 2 cut(s) 795, 924
BspFNI CGCG 1 cut(s) 1286
BspLI GGNNCC 5 cut(s) 7, 194, 388, 834, 1409
BspMAI CTGCAG 1 cut(s) 766
BspPI GGATC 4 cut(s) 827, 840, 1402, 1415
BspQI GCTCTTC 1 cut(s) 1047
BspT107I GGYRCC 1 cut(s) 386
BsrDI GCAATG 3 cut(s) 586, 742, 801
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 9 cut(s) 139, 209, 357, 451, 509, 796, 822, 1170, 1223
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 5 cut(s) 196, 235, 306, 828, 1262
BssMI GATC 3 cut(s) 832, 905, 1407
BssNAI GTATAC 1 cut(s) 1305
BssNI GRCGYC 2 cut(s) 1029, 1258
BssSI CACGAG 1 cut(s) 1364
BssT1I CCWWGG 2 cut(s) 828, 1262
Bst1107I GTATAC 1 cut(s) 1305
Bst2BI CACGAG 1 cut(s) 1364
Bst2UI CCWGG 3 cut(s) 99, 198, 237
Bst4CI ACNGT 5 cut(s) 64, 413, 731, 1063, 1105
Bst6I CTCTTC 2 cut(s) 867, 1047
BstACI GRCGYC 2 cut(s) 1029, 1258
BstAPI GCANNNNNTGC 1 cut(s) 37
BstC8I GCNNGC 5 cut(s) 16, 29, 448, 769, 1157
BstDEI CTNAG 3 cut(s) 626, 782, 911
BstEII GGTNACC 1 cut(s) 1178
BstF5I GGATG 5 cut(s) 157, 245, 379, 552, 693
BstFNI CGCG 1 cut(s) 1286
BstKTI GATC 3 cut(s) 835, 908, 1410
BstMAI GTCTC 2 cut(s) 785, 1097
BstMBI GATC 3 cut(s) 832, 905, 1407
BstMCI CGRYCG 1 cut(s) 1055
BstMWI GCNNNNNNNGC 3 cut(s) 24, 37, 46
BstNI CCWGG 3 cut(s) 99, 198, 237
BstNSI RCATGY 2 cut(s) 771, 1396
BstPI GGTNACC 1 cut(s) 1178
BstSCI CCNGG 6 cut(s) 97, 196, 235, 305, 489, 1054
BstSFI CTRYAG 2 cut(s) 762, 1104
BstUI CGCG 1 cut(s) 1286
BstV1I GCAGC 3 cut(s) 33, 455, 776
BstX2I RGATCY 3 cut(s) 832, 905, 1407
BstXI CCANNNNNNTGG 2 cut(s) 342, 829
BstYI RGATCY 3 cut(s) 832, 905, 1407
BstZ17I GTATAC 1 cut(s) 1305
BsuI GTATCC 1 cut(s) 745
BsuRI GGCC 5 cut(s) 82, 234, 276, 356, 1155
BtsCI GGATG 5 cut(s) 157, 245, 379, 552, 693
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 6 cut(s) 60, 418, 759, 790, 803, 1180
Cac8I GCNNGC 5 cut(s) 16, 29, 448, 769, 1157
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 4 cut(s) 81, 233, 275, 423
CseI GACGC 2 cut(s) 1018, 1247
Csp6I GTAC 9 cut(s) 328, 454, 501, 623, 712, 825, 1216, 1291, 1369
CviAII CATG 6 cut(s) 342, 768, 1046, 1184, 1189, 1393
CviQI GTAC 9 cut(s) 328, 454, 501, 623, 712, 825, 1216, 1291, 1369
DdeI CTNAG 3 cut(s) 626, 782, 911
DpnI GATC 3 cut(s) 834, 907, 1409
DpnII GATC 3 cut(s) 832, 905, 1407
DraI TTTAAA 1 cut(s) 252
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 867, 1047
EarI CTCTTC 2 cut(s) 867, 1047
Eco130I CCWWGG 2 cut(s) 828, 1262
Eco147I AGGCCT 1 cut(s) 1155
Eco32I GATATC 1 cut(s) 1319
Eco47I GGWCC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 207
Eco91I GGTNACC 1 cut(s) 1178
EcoO109I RGGNCCY 1 cut(s) 233
EcoO65I GGTNACC 1 cut(s) 1178
EcoRII CCWGG 3 cut(s) 97, 196, 235
EcoRV GATATC 1 cut(s) 1319
EcoT14I CCWWGG 2 cut(s) 828, 1262
ErhI CCWWGG 2 cut(s) 828, 1262
FaeI CATG 6 cut(s) 345, 771, 1049, 1187, 1192, 1396
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 1 cut(s) 838
FatI CATG 6 cut(s) 341, 767, 1045, 1183, 1188, 1392
FauI CCCGC 1 cut(s) 1279
FauNDI CATATG 2 cut(s) 532, 1330
FblI GTMKAC 3 cut(s) 513, 1107, 1304
Fnu4HI GCNGC 5 cut(s) 47, 417, 444, 765, 897
FokI GGATG 5 cut(s) 164, 252, 386, 539, 700
Fsp4HI GCNGC 5 cut(s) 47, 417, 444, 765, 897
GluI GCNGC 5 cut(s) 47, 417, 444, 765, 897
GsuI CTGGAG 2 cut(s) 219, 609
HaeIII GGCC 5 cut(s) 82, 234, 276, 356, 1155
HapII CCGG 5 cut(s) 108, 306, 390, 491, 1055
HgaI GACGC 2 cut(s) 1018, 1247
Hin1I GRCGYC 2 cut(s) 1029, 1258
Hin1II CATG 6 cut(s) 345, 771, 1049, 1187, 1192, 1396
HinfI GANTC 7 cut(s) 182, 258, 527, 544, 591, 704, 716
HpaII CCGG 5 cut(s) 108, 306, 390, 491, 1055
HphI GGTGA 3 cut(s) 122, 1190, 1350
Hpy166II GTNNAC 4 cut(s) 514, 1108, 1293, 1305
Hpy188I TCNGA 5 cut(s) 187, 703, 914, 1006, 1143
Hpy188III TCNNGA 5 cut(s) 563, 836, 928, 1244, 1364
Hpy8I GTNNAC 4 cut(s) 514, 1108, 1293, 1305
Hpy99I CGWCG 1 cut(s) 1127
HpyAV CCTTC 3 cut(s) 130, 182, 1019
HpyCH4III ACNGT 5 cut(s) 64, 413, 731, 1063, 1105
HpyCH4IV ACGT 1 cut(s) 1086
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 37, 46
HpyF3I CTNAG 3 cut(s) 626, 782, 911
HpySE526I ACGT 1 cut(s) 1086
Hsp92I GRCGYC 2 cut(s) 1029, 1258
Hsp92II CATG 6 cut(s) 345, 771, 1049, 1187, 1192, 1396
Kzo9I GATC 3 cut(s) 832, 905, 1407
LguI GCTCTTC 1 cut(s) 1047
LmnI GCTCC 2 cut(s) 200, 1346
Lsp1109I GCAGC 3 cut(s) 33, 455, 776
LweI GCATC 2 cut(s) 364, 1108
MaeII ACGT 1 cut(s) 1086
MaeIII GTNAC 3 cut(s) 817, 1178, 1352
MalI GATC 3 cut(s) 834, 907, 1409
MboI GATC 3 cut(s) 832, 905, 1407
MboII GAAGA 9 cut(s) 157, 253, 539, 881, 884, 890, 899, 915, 1034
MflI RGATCY 3 cut(s) 832, 905, 1407
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 5 cut(s) 270, 672, 954, 985, 1160
MlyI GAGTC 3 cut(s) 191, 600, 725
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 9 cut(s) 224, 233, 620, 868, 1030, 1247, 1293, 1371, 1375
MseI TTAA 2 cut(s) 251, 1341
MspI CCGG 5 cut(s) 108, 306, 390, 491, 1055
MspR9I CCNGG 6 cut(s) 99, 198, 237, 307, 491, 1056
MssI GTTTAAAC 1 cut(s) 252
MvaI CCWGG 3 cut(s) 99, 198, 237
MvnI CGCG 1 cut(s) 1286
MwoI GCNNNNNNNGC 3 cut(s) 24, 37, 46
NciI CCSGG 3 cut(s) 307, 491, 1056
NdeI CATATG 2 cut(s) 532, 1330
NdeII GATC 3 cut(s) 832, 905, 1407
NlaIII CATG 6 cut(s) 345, 771, 1049, 1187, 1192, 1396
NlaIV GGNNCC 5 cut(s) 7, 194, 388, 834, 1409
NmuCI GTSAC 2 cut(s) 1178, 1352
NspI RCATGY 2 cut(s) 771, 1396
PaeI GCATGC 1 cut(s) 771
PceI AGGCCT 1 cut(s) 1155
PciI ACATGT 1 cut(s) 1392
PciSI GCTCTTC 1 cut(s) 1047
PfeI GAWTC 4 cut(s) 258, 527, 544, 704
PflMI CCANNNNNTGG 3 cut(s) 688, 1189, 1205
PkrI GCNGC 5 cut(s) 48, 418, 445, 766, 898
PleI GAGTC 3 cut(s) 190, 599, 724
PmeI GTTTAAAC 1 cut(s) 252
PpsI GAGTC 3 cut(s) 190, 599, 724
PscI ACATGT 1 cut(s) 1392
Psp1406I AACGTT 1 cut(s) 1086
Psp6I CCWGG 3 cut(s) 97, 196, 235
PspEI GGTNACC 1 cut(s) 1178
PspGI CCWGG 3 cut(s) 97, 196, 235
PspN4I GGNNCC 5 cut(s) 7, 194, 388, 834, 1409
PspPI GGNCC 4 cut(s) 81, 233, 275, 423
PstI CTGCAG 1 cut(s) 766
PsuI RGATCY 3 cut(s) 832, 905, 1407
RsaI GTAC 9 cut(s) 329, 455, 502, 624, 713, 826, 1217, 1292, 1370
RsaNI GTAC 9 cut(s) 328, 454, 501, 623, 712, 825, 1216, 1291, 1369
SapI GCTCTTC 1 cut(s) 1047
SaqAI TTAA 2 cut(s) 251, 1341
SatI GCNGC 5 cut(s) 47, 417, 444, 765, 897
Sau3AI GATC 3 cut(s) 832, 905, 1407
Sau96I GGNCC 4 cut(s) 81, 233, 275, 423
ScaI AGTACT 1 cut(s) 1217
SchI GAGTC 3 cut(s) 191, 600, 725
ScrFI CCNGG 6 cut(s) 99, 198, 237, 307, 491, 1056
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 2 cut(s) 364, 1108
SfcI CTRYAG 2 cut(s) 762, 1104
SinI GGWCC 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
SphI GCATGC 1 cut(s) 771
Sse9I AATT 5 cut(s) 270, 672, 954, 985, 1160
SseBI AGGCCT 1 cut(s) 1155
SsiI CCGC 4 cut(s) 417, 635, 897, 1286
SspI AATATT 2 cut(s) 366, 1071
StuI AGGCCT 1 cut(s) 1155
StyD4I CCNGG 6 cut(s) 97, 196, 235, 305, 489, 1054
StyI CCWWGG 2 cut(s) 828, 1262
TaaI ACNGT 5 cut(s) 64, 413, 731, 1063, 1105
TaiI ACGT 1 cut(s) 1089
TaqI TCGA 6 cut(s) 159, 180, 594, 1051, 1245, 1398
TaqII GACCGA 1 cut(s) 1160
TasI AATT 5 cut(s) 270, 672, 954, 985, 1160
TatI WGTACW 4 cut(s) 453, 500, 1215, 1368
TauI GCSGC 2 cut(s) 419, 899
TfiI GAWTC 4 cut(s) 258, 527, 544, 704
Tru1I TTAA 2 cut(s) 251, 1341
Tru9I TTAA 2 cut(s) 251, 1341
TscAI CASTG 6 cut(s) 67, 418, 766, 790, 803, 1180
TseFI GTSAC 2 cut(s) 1178, 1352
TseI GCWGC 3 cut(s) 46, 443, 764
Tsp45I GTSAC 2 cut(s) 1178, 1352
TspDTI ATGAA 9 cut(s) 65, 168, 498, 519, 557, 597, 924, 1034, 1038
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 6 cut(s) 67, 418, 766, 790, 803, 1180
Van91I CCANNNNNTGG 3 cut(s) 688, 1189, 1205
VpaK11BI GGWCC 1 cut(s) 423
XceI RCATGY 2 cut(s) 771, 1396
XmiI GTMKAC 3 cut(s) 513, 1107, 1304
ZrmI AGTACT 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.