Rorug05G0534600

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018369
Physical Location & Seq
Forward (+)
72249344 .. 72289450
40107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0534600.1

Sequence Viewer

Length: 3183 bp
ATGTCACTGGTGGTGTGGTTTTGGTATGCTGCAGCAGCCTGCAGCATATTCATCTCTGGGTTTGTTGCACAGGCTCAACCCACCACAGATCCCTCTGAAGTGAGAGCGCTGAATGCAATCTTTCAGAAATGGAATATTACAGCCAATACAAAGCAATGGAACACAACTGGTGATCCATGCAGTGAACTTGCGGTAGATGAAGATGATGAGACCGCTTATTTCCATGATGATGATTACAATCTCCTCGTCAAATGCAACTGCTCTTATGATTCAAACTCAACTTGCCACATTACCCAACTGAAAGCAATCACTTTGGACATTGTTGCTCCAATTCCAGAAGAGCTCTATGCTTTGACTTTCCTTCAATATCTGAATGTGAAAAAAAATTACTTGACAGGACCCATCTCTGCATCGATTGGAAATCTAACCAGCATCAAATACTTGGACTTGGGACACAACGACTTATCAGGGGAACTACCAAAGGAAATTGAAAAACTCACCAATTTGACATTCTTGGGTGTTGGGACACTCAACCTTTCTGGTTCTCTGCCTCCTGAGATGGGGTATTTAAAAACATTAAAAGAGATTAAAATTGATAATTCTGGACTTAGCGGTGAGATTCCACCAACATTTGCTAATCTAACAAACTTGGAAATATTTTGGGCAACAGATATAAACCTCACTGGCAGGATACCGGACTTCATAGGAGATTGGACAAAGCTTACTAAATTGAGGTTACAGGGAACCTCCTTTGAAGGTTCCATACCATCTTCACTGTCCAGATTGACTCTTTTGAAAGAGCTGCGAATTACTGATTTAACGATTTCCAATGACAACTCTTCTCTTGGATTTATCAAGAATATGACGTCTCTAGAAGTTTTAATGCTGAGGAATAACAATATTTCTGATTCAATTCCTTCCAACATTGGAGATTACCGAAAATTGGTTCACCTGGATTTGAGCTTCAACAATTTGATTGGACAAATACCAGCCTCACTATTTGAGATGAATATGCTCGCCATCCTGTTTCTTGGAAACAATAAGTTGAATGGTAGCCTTCCTGAGTCTAAAAGCTCATCTCTTCTCTTTGTAGATTTGTCTTACAATAATCTAGTAGGCCAGAGCTTTCCGAGTTGGGTCAACGAACAAAGCCTAGACCTTAACTTAGTGGCCAACAACTTCAGCATAGACAGTTCAGACAGCAGTGGTCTACCTTCAGGGTTGAATTGCCTTCAAAGGGGCTTCCCTTGCCACCGTGGTAGTCCAGTTTACTATAACTTCATGATTAACTGCGGTGGTCCTCAGGCTACGATATCCAATGGGACTGTGTATGAGAAGGACATTGAGCCCCTTGGTCCAGCTACATATTATGTGACTGACACAAACAGATGGGGAGTTAGCAATGTCGGTCTTAAGACACAGTTTGTAGCTGCATCATATTTGACTGGGACCAACATTCCAAAGTATACAATATCCACTTCATCTCCAATCGGAAATACTTCAGACCCTACGCTATTTCAGAGTGCAAGGATCTCTGCTTCATCACTCAGATACTATGGCTTGGGTCTTGAGAATGGAAAGTATACCGTGAAGCTTGAATTTGCAGAACAAGCTATCCTAGATACCCCTAAAAGAAAAAGTCATGGAAGACGTGTGTTTGATATATATATCCAGGATGTTCTTGTTGGTAAAGATTTTGATATACGGAAGGAGACAAATAAGACATCTTTGCTAGCTATTGAAAAGGTATACAAGGCTCAGGTGGTTTCAGAGAATTACCTTGAAATCCATTTCTTCTGGGCTGGAAAGGGGACTTGCTGTATACCAGAGGAGGGTACTTATGGACCTCTTGTTTCAGCCATCAGTGCTACACCTGAATTCAAACCTACTGTCAGCAACAAGCTGCCAAGTAGTAAGAAGAATAGGACTGGGCTTATTGTGGGAATTGTTGTTGGTTGTGGTGTTTTAATTCTGGTGGTGCTGCTTTTATATATTGTTCAAGGAAGAAAGAGGCACAACACTGATGATGACTATGATGAAGAGCTGTTGGGAATAGATCTTGGACCTCTCACTTTCAGTTTTTCTGAACTCAAGGCAGCTACAAATGACTTTAGTCCAGATAATAAGCTTGGAGAGGGAGGATTTGGGCCTGTCTTCCAAGGTGCTCTAAATGATGGAAGAGAAATTGCTGTAAAACAATTGTCTGCATCATCCCACCAAGGAAAGAACCAGTTTGTGACTGAGATTGCCACTATATCTTCTGTGCGACACAATAACCTAGTAAATTTGTATGGATTCTGCACCGAGGGAGCTAAACGGCTCCTTGTCTATGAGTATCTGGAGAACAAGAGTCTTGATCAAGCATTATTTGGAAAAAGAACCTTGCATCTTGATTGGCAGACACGTTTTGATATATGCTTGGGTGTAGCTAGAGGTTTAACTTATCTTCATGAAGAATCAAGGCTTCGAATTGTACACAGAGATGTGAAGGCAAGTAACATTCTGCTTGACTCTAATCTCATTGCCAAAATATCAGATTTTGGTTTGGCGAAGCATTATGATGATAAAAGGACCCACATGAGTACTCGGGTTGCAGGAACATTTGGGTACCTTGCACCGGAATATGCTATGCGTGGACACTTGACAGAGAAAACCGATGTGTTTGCCTATGGTGTTGTCATTCTAGAAATTGTCAGCGGTAGGCCAAATTCTGATCCAAGTTTGGAAGGCGATATGGTTTATCTTCTTGAATTGGCTTGGAACTTGCATGAAAACAAACGTGAAGTTGATCTAGTAGACTCTAGATTATCCGAATTCAATGAGGAAGAAGCAAGACGAATTATCAACATAGGACTTTTGTGCACTCAATCATCACCAACGATACGACCACCTATGTCTCGCGTGATCGGAATGCTTGCAGGAGATATTGAAGTGACTTCTGCTATTTCAAAGCCCGGTTACTTGACAGACTGGAGATTTGATGATGTAACTACTATCACCAGCCAAACGAAATTTGGTGATGGGAGTACTGGCAACAGTCTAGGAACTGATATGTCAACTAGAGGAACTGACTTCAGCTATTACAACTCATCGGCAAGTACAAGCGTGATGGGGCATGCAGGGCAATTACCTTCAAATGCTACTCTTCCCATACTCAAAAATACAAATGGTGATGGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

1060

Amino Acids

116.85

Weight (kDa)

5.41

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 138 - 246 2.3e-11 Leucine-rich repeat region
LRR_14 PF23598 257 - 348 1.8e-06 Leucine-rich repeat region
LRR_4 PF12799 289 - 330 3.3e-06 Leucine Rich repeats (2 copies)
Malectin PF11721 427 - 621 1.6e-37 Malectin domain
PK_Tyr_Ser-Thr PF07714 707 - 969 2.2e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 707 - 904 1.4e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018369)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03522
rosa_chinensis RchiOBHm_Chr6g0248941
rosa_laevigata RLG00000015107 RLG00000015108
rosa_multiflora Rmu_sc0003692.1_g000031
rosa_roxburghii Rroxscaffold_7G00212810
rosa_rugosa Rorug05G0534500 Rorug05G0534600
rosa_samantha Rh6AG051800
rosa_wichuraiana Rw6G004600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 869
Acc65I GGTACC 1 cut(s) 2607
AccB1I GGYRCC 1 cut(s) 2607
AccI GTMKAC 6 cut(s) 1210, 1466, 1583, 1749, 1822, 2796
AccII CGCG 1 cut(s) 2901
AciI CCGC 5 cut(s) 191, 213, 612, 1293, 2697
AclWI GGATC 4 cut(s) 83, 167, 1538, 2708
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 6 cut(s) 1598, 1877, 2284, 2707, 2813, 3011
AcuI CTGAAG 5 cut(s) 117, 1165, 1200, 1485, 3058
AcyI GRCGYC 1 cut(s) 866
AfaI GTAC 6 cut(s) 1837, 2475, 2584, 2609, 3028, 3100
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 5 cut(s) 560, 943, 1237, 1832, 2617
AflII CTTAAG 1 cut(s) 1412
AflIII ACRYGT 2 cut(s) 1651, 2402
AjiI CACGTC 1 cut(s) 1652
AjnI CCWGG 2 cut(s) 951, 1671
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AloI GAACNNNNNNTCC 2 cut(s) 1599, 1631
Alw21I GWGCWC 3 cut(s) 345, 2167, 2864
Alw26I GTCTC 4 cut(s) 203, 873, 1706, 2901
Alw44I GTGCAC 1 cut(s) 2860
AlwI GGATC 4 cut(s) 83, 167, 1538, 2708
Ama87I CYCGRG 1 cut(s) 2586
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 4 cut(s) 1117, 1170, 2147, 2702
ApaLI GTGCAC 1 cut(s) 2860
ApeKI GCWGC 9 cut(s) 29, 32, 35, 42, 802, 1430, 1903, 1981, 2096
ApoI RAATTY 6 cut(s) 1598, 1877, 2284, 2707, 2813, 3011
ArsI GACNNNNNNTTYG 4 cut(s) 1875, 1907, 2960, 2992
Asp700I GAANNNNTTC 1 cut(s) 1498
Asp718I GGTACC 1 cut(s) 2607
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 8 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2147, 2571
AsuC2I CCSGG 1 cut(s) 2955
AsuHPI GGTGA 8 cut(s) 182, 490, 626, 941, 2865, 2989, 3029, 3182
AsuII TTCGAA 1 cut(s) 2467
AsuNHI GCTAGC 1 cut(s) 1732
AvaI CYCGRG 1 cut(s) 2586
AvaII GGWCC 7 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2571
AxyI CCTNAGG 1 cut(s) 1302
BaeGI GKGCMC 1 cut(s) 2864
BalI TGGCCA 1 cut(s) 1172
BanI GGYRCC 1 cut(s) 2607
BanII GRGCYC 2 cut(s) 345, 1350
BarI GAAGNNNNNNTAC 2 cut(s) 747, 779
BbsI GAAGAC 2 cut(s) 1654, 2146
Bbv12I GWGCWC 3 cut(s) 345, 2167, 2864
BbvCI CCTCAGC 1 cut(s) 887
BbvI GCAGC 9 cut(s) 16, 44, 47, 54, 789, 1417, 1890, 1968, 2108
BceAI ACGGC 1 cut(s) 2333
BcgI CGANNNNNNTGC 2 cut(s) 2645, 2679
BciT130I CCWGG 2 cut(s) 953, 1673
BciVI GTATCC 1 cut(s) 684
BclI TGATCA 1 cut(s) 2356
BcnI CCSGG 1 cut(s) 2955
BcoDI GTCTC 4 cut(s) 203, 873, 1706, 2901
BfmI CTRYAG 2 cut(s) 30, 40
BfoI RGCGCY 1 cut(s) 110
BfrI CTTAAG 1 cut(s) 1412
BfuI GTATCC 1 cut(s) 684
BglII AGATCT 1 cut(s) 2056
BisI GCNGC 9 cut(s) 30, 33, 36, 43, 803, 1431, 1904, 1982, 2097
BlsI GCNGC 9 cut(s) 31, 34, 37, 44, 804, 1432, 1905, 1983, 2098
BmcAI AGTACT 2 cut(s) 2584, 3028
Bme1390I CCNGG 3 cut(s) 953, 1673, 2955
Bme18I GGWCC 7 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2571
BmeT110I CYCGRG 1 cut(s) 2586
BmgBI CACGTC 1 cut(s) 1652
BmgT120I GGNCC 8 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2147, 2571
BmiI GGNNCC 7 cut(s) 400, 745, 760, 1450, 2321, 2573, 2609
BmrFI CCNGG 3 cut(s) 953, 1673, 2955
BmrI ACTGGG 2 cut(s) 1455, 1938
BmsI GCATC 5 cut(s) 419, 441, 1442, 2216, 2395
BmtI GCTAGC 1 cut(s) 1736
BmuI ACTGGG 2 cut(s) 1455, 1938
BoxI GACNNNNGTC 1 cut(s) 2112
BpiI GAAGAC 2 cut(s) 1654, 2146
BpmI CTGGAG 2 cut(s) 2360, 2992
Bpu10I CCTNAGC 2 cut(s) 887, 1758
Bpu14I TTCGAA 1 cut(s) 2467
BpuEI CTTGAG 2 cut(s) 1589, 2075
BpuMI CCSGG 1 cut(s) 2955
Bsa29I ATCGAT 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 866
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 5 cut(s) 1255, 1351, 2158, 2218, 2304
BsaWI WCCGGW 2 cut(s) 694, 2617
BsaXI ACNNNNNCTCC 2 cut(s) 1468, 1498
Bsc4I CCNNNNNNNGG 5 cut(s) 560, 943, 1237, 1832, 2617
Bse1I ACTGG 9 cut(s) 12, 172, 688, 1265, 1450, 1933, 2230, 2975, 3034
Bse21I CCTNAGG 1 cut(s) 1302
Bse3DI GCAATG 3 cut(s) 161, 1408, 2520
Bse8I GATNNNNATC 1 cut(s) 237
BseBI CCWGG 2 cut(s) 953, 1673
BseCI ATCGAT 1 cut(s) 413
BseDI CCNNGG 5 cut(s) 1255, 1351, 2158, 2218, 2304
BseGI GGATG 3 cut(s) 1020, 1681, 2210
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 5 cut(s) 560, 943, 1237, 1832, 2617
BseMI GCAATG 3 cut(s) 161, 1408, 2520
BseMII CTCAG 7 cut(s) 546, 878, 1053, 1316, 1561, 1772, 2232
BseNI ACTGG 9 cut(s) 12, 172, 688, 1265, 1450, 1933, 2230, 2975, 3034
BseRI GAGGAG 2 cut(s) 233, 1844
BseSI GKGCMC 1 cut(s) 2864
BseXI GCAGC 9 cut(s) 16, 44, 47, 54, 789, 1417, 1890, 1968, 2108
BsgI GTGCAG 1 cut(s) 2284
Bsh1236I CGCG 1 cut(s) 2901
BshFI GGCC 4 cut(s) 1119, 1172, 2149, 2704
BshNI GGYRCC 1 cut(s) 2607
BshVI ATCGAT 1 cut(s) 413
BsiHKAI GWGCWC 3 cut(s) 345, 2167, 2864
BsiHKCI CYCGRG 1 cut(s) 2586
BsiSI CCGG 3 cut(s) 695, 2618, 2955
BslFI GGGAC 5 cut(s) 465, 538, 1336, 1462, 1825
BslI CCNNNNNNNGG 5 cut(s) 560, 943, 1237, 1832, 2617
BsmAI GTCTC 4 cut(s) 203, 873, 1706, 2901
BsmBI CGTCTC 1 cut(s) 873
BsmFI GGGAC 5 cut(s) 465, 538, 1336, 1462, 1825
BsmI GAATGC 2 cut(s) 118, 2916
BsnI GGCC 4 cut(s) 1119, 1172, 2149, 2704
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 2586
Bsp119I TTCGAA 1 cut(s) 2467
Bsp1286I GDGCHC 4 cut(s) 345, 1350, 2167, 2864
Bsp1407I TGTACA 1 cut(s) 2473
Bsp143I GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
BspACI CCGC 5 cut(s) 191, 213, 612, 1293, 2697
BspANI GGCC 4 cut(s) 1119, 1172, 2149, 2704
BspCNI CTCAG 7 cut(s) 547, 879, 1054, 1315, 1560, 1771, 2233
BspDI ATCGAT 1 cut(s) 413
BspFNI CGCG 1 cut(s) 2901
BspHI TCATGA 2 cut(s) 1281, 2449
BspLI GGNNCC 7 cut(s) 400, 745, 760, 1450, 2321, 2573, 2609
BspMAI CTGCAG 2 cut(s) 34, 44
BspOI GCTAGC 1 cut(s) 1736
BspPI GGATC 4 cut(s) 83, 167, 1538, 2708
BspQI GCTCTTC 2 cut(s) 333, 2034
BspT104I TTCGAA 1 cut(s) 2467
BspT107I GGYRCC 1 cut(s) 2607
BspTI CTTAAG 1 cut(s) 1412
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 3 cut(s) 161, 1408, 2520
BsrGI TGTACA 1 cut(s) 2473
BsrI ACTGG 9 cut(s) 12, 172, 688, 1265, 1450, 1933, 2230, 2975, 3034
BssECI CCNNGG 5 cut(s) 1255, 1351, 2158, 2218, 2304
BssMI GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
BssNAI GTATAC 4 cut(s) 1467, 1584, 1750, 1823
BssNI GRCGYC 1 cut(s) 866
BssT1I CCWWGG 3 cut(s) 1351, 2158, 2218
Bst1107I GTATAC 4 cut(s) 1467, 1584, 1750, 1823
Bst2UI CCWGG 2 cut(s) 953, 1673
Bst4CI ACNGT 8 cut(s) 777, 1193, 1256, 1327, 1422, 1588, 1891, 3038
Bst6I CTCTTC 6 cut(s) 333, 844, 1086, 2034, 2173, 3150
BstACI GRCGYC 1 cut(s) 866
BstAFI CTTAAG 1 cut(s) 1412
BstAUI TGTACA 1 cut(s) 2473
BstBI TTCGAA 1 cut(s) 2467
BstC8I GCNNGC 5 cut(s) 40, 1017, 1734, 2916, 3117
BstDEI CTNAG 9 cut(s) 555, 608, 887, 1062, 1165, 1302, 1547, 1758, 2241
BstDSI CCRYGG 1 cut(s) 1255
BstF5I GGATG 3 cut(s) 1020, 1681, 2210
BstFNI CGCG 1 cut(s) 2901
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 8 cut(s) 91, 175, 1533, 2059, 2359, 2716, 2791, 2907
BstMAI GTCTC 4 cut(s) 203, 873, 1706, 2901
BstMBI GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
BstMWI GCNNNNNNNGC 6 cut(s) 35, 113, 1248, 1610, 1865, 3121
BstNI CCWGG 2 cut(s) 953, 1673
BstNSI RCATGY 1 cut(s) 3119
BstPAI GACNNNNGTC 1 cut(s) 2112
BstSCI CCNGG 3 cut(s) 951, 1671, 2953
BstSFI CTRYAG 2 cut(s) 30, 40
BstSLI GKGCMC 1 cut(s) 2864
BstUI CGCG 1 cut(s) 2901
BstV1I GCAGC 9 cut(s) 16, 44, 47, 54, 789, 1417, 1890, 1968, 2108
BstV2I GAAGAC 2 cut(s) 1654, 2146
BstX2I RGATCY 3 cut(s) 88, 1530, 2056
BstYI RGATCY 3 cut(s) 88, 1530, 2056
BstZ17I GTATAC 4 cut(s) 1467, 1584, 1750, 1823
Bsu15I ATCGAT 1 cut(s) 413
Bsu36I CCTNAGG 1 cut(s) 1302
BsuI GTATCC 1 cut(s) 684
BsuRI GGCC 4 cut(s) 1119, 1172, 2149, 2704
BsuTUI ATCGAT 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 1255
BtrI CACGTC 1 cut(s) 1652
BtsCI GGATG 3 cut(s) 1020, 1681, 2210
BtsI GCAGTG 2 cut(s) 187, 1210
BtsIMutI CAGTG 7 cut(s) 5, 187, 681, 773, 1210, 1870, 2019
Cac8I GCNNGC 5 cut(s) 40, 1017, 1734, 2916, 3117
CciI TCATGA 2 cut(s) 1281, 2449
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 8 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2147, 2571
ClaI ATCGAT 1 cut(s) 413
Csp6I GTAC 6 cut(s) 1836, 2474, 2583, 2608, 3027, 3099
CviAII CATG 8 cut(s) 177, 224, 1282, 1643, 2450, 2578, 2768, 3116
CviQI GTAC 6 cut(s) 1836, 2474, 2583, 2608, 3027, 3099
DdeI CTNAG 9 cut(s) 555, 608, 887, 1062, 1165, 1302, 1547, 1758, 2241
DpnI GATC 8 cut(s) 90, 174, 1532, 2058, 2358, 2715, 2790, 2906
DpnII GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
DraI TTTAAA 1 cut(s) 570
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 6 cut(s) 333, 844, 1086, 2034, 2173, 3150
EarI CTCTTC 6 cut(s) 333, 844, 1086, 2034, 2173, 3150
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 1351, 2158, 2218
Eco24I GRGCYC 2 cut(s) 345, 1350
Eco31I GGTCTC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 1314
Eco47I GGWCC 7 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2571
Eco47III AGCGCT 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 5 cut(s) 117, 1165, 1200, 1485, 3058
Eco81I CCTNAGG 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 2586
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 2 cut(s) 398, 2571
EcoRI GAATTC 2 cut(s) 1877, 2813
EcoRII CCWGG 2 cut(s) 951, 1671
EcoRV GATATC 1 cut(s) 1314
EcoT14I CCWWGG 3 cut(s) 1351, 2158, 2218
EcoT38I GRGCYC 2 cut(s) 345, 1350
ErhI CCWWGG 3 cut(s) 1351, 2158, 2218
Esp3I CGTCTC 1 cut(s) 873
FaeI CATG 8 cut(s) 180, 227, 1285, 1646, 2453, 2581, 2771, 3119
FaqI GGGAC 5 cut(s) 465, 538, 1336, 1462, 1825
FatI CATG 8 cut(s) 176, 223, 1281, 1642, 2449, 2577, 2767, 3115
FbaI TGATCA 1 cut(s) 2356
FblI GTMKAC 6 cut(s) 1210, 1466, 1583, 1749, 1822, 2796
Fnu4HI GCNGC 9 cut(s) 30, 33, 36, 43, 803, 1431, 1904, 1982, 2097
FokI GGATG 3 cut(s) 1007, 1688, 2197
FriOI GRGCYC 2 cut(s) 345, 1350
Fsp4HI GCNGC 9 cut(s) 30, 33, 36, 43, 803, 1431, 1904, 1982, 2097
GlaI GCGC 1 cut(s) 108
GluI GCNGC 9 cut(s) 30, 33, 36, 43, 803, 1431, 1904, 1982, 2097
GsuI CTGGAG 2 cut(s) 2360, 2992
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 4 cut(s) 1119, 1172, 2149, 2704
HapII CCGG 3 cut(s) 695, 2618, 2955
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 866
Hin1II CATG 8 cut(s) 180, 227, 1285, 1646, 2453, 2581, 2771, 3119
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 1141, 3057
HindII GTYRAC 2 cut(s) 1141, 3057
HindIII AAGCTT 3 cut(s) 719, 1592, 2126
HpaII CCGG 3 cut(s) 695, 2618, 2955
HphI GGTGA 8 cut(s) 182, 490, 626, 941, 2865, 2989, 3029, 3182
HpyCH4III ACNGT 8 cut(s) 777, 1193, 1256, 1327, 1422, 1588, 1891, 3038
HpyCH4IV ACGT 4 cut(s) 866, 1651, 2404, 2779
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 113, 1248, 1610, 1865, 3121
HpyF3I CTNAG 9 cut(s) 555, 608, 887, 1062, 1165, 1302, 1547, 1758, 2241
HpySE526I ACGT 4 cut(s) 866, 1651, 2404, 2779
Hsp92I GRCGYC 1 cut(s) 866
Hsp92II CATG 8 cut(s) 180, 227, 1285, 1646, 2453, 2581, 2771, 3119
HspAI GCGC 1 cut(s) 107
KpnI GGTACC 1 cut(s) 2611
Ksp22I TGATCA 1 cut(s) 2356
Kzo9I GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
LguI GCTCTTC 2 cut(s) 333, 2034
LmnI GCTCC 3 cut(s) 331, 2309, 2325
Lsp1109I GCAGC 9 cut(s) 16, 44, 47, 54, 789, 1417, 1890, 1968, 2108
LweI GCATC 5 cut(s) 419, 441, 1442, 2216, 2395
MaeII ACGT 4 cut(s) 866, 1651, 2404, 2779
MaeIII GTNAC 8 cut(s) 3, 735, 1372, 2236, 2495, 2932, 2957, 2986
MalI GATC 8 cut(s) 90, 174, 1532, 2058, 2358, 2715, 2790, 2906
MboI GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
MfeI CAATTG 1 cut(s) 2198
MflI RGATCY 3 cut(s) 88, 1530, 2056
MhlI GDGCHC 4 cut(s) 345, 1350, 2167, 2864
MlsI TGGCCA 1 cut(s) 1172
MluNI TGGCCA 1 cut(s) 1172
MlyI GAGTC 5 cut(s) 781, 1073, 2359, 2504, 2792
MmeI TCCRAC 1 cut(s) 945
Mox20I TGGCCA 1 cut(s) 1172
MroXI GAANNNNTTC 1 cut(s) 1498
MscI TGGCCA 1 cut(s) 1172
MslI CAYNNNNRTG 3 cut(s) 228, 2481, 2559
Msp20I TGGCCA 1 cut(s) 1172
MspA1I CMGCKG 1 cut(s) 2697
MspCI CTTAAG 1 cut(s) 1412
MspI CCGG 3 cut(s) 695, 2618, 2955
MspR9I CCNGG 3 cut(s) 953, 1673, 2955
MunI CAATTG 1 cut(s) 2198
Mva1269I GAATGC 2 cut(s) 118, 2916
MvaI CCWGG 2 cut(s) 953, 1673
MvnI CGCG 1 cut(s) 2901
MwoI GCNNNNNNNGC 6 cut(s) 35, 113, 1248, 1610, 1865, 3121
NciI CCSGG 1 cut(s) 2955
NdeII GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
NheI GCTAGC 1 cut(s) 1732
NlaIII CATG 8 cut(s) 180, 227, 1285, 1646, 2453, 2581, 2771, 3119
NlaIV GGNNCC 7 cut(s) 400, 745, 760, 1450, 2321, 2573, 2609
NmuCI GTSAC 4 cut(s) 3, 1372, 2236, 2932
NspI RCATGY 1 cut(s) 3119
NspV TTCGAA 1 cut(s) 2467
PaeI GCATGC 1 cut(s) 3119
PagI TCATGA 2 cut(s) 1281, 2449
PciSI GCTCTTC 2 cut(s) 333, 2034
PctI GAATGC 2 cut(s) 118, 2916
PdmI GAANNNNTTC 1 cut(s) 1498
PfeI GAWTC 5 cut(s) 269, 619, 908, 2295, 2456
PfoI TCCNGGA 1 cut(s) 1671
PkrI GCNGC 9 cut(s) 31, 34, 37, 44, 804, 1432, 1905, 1983, 2098
PleI GAGTC 5 cut(s) 781, 1072, 2358, 2504, 2792
PpsI GAGTC 5 cut(s) 781, 1072, 2358, 2504, 2792
PpuMI RGGWCCY 2 cut(s) 398, 2571
PshAI GACNNNNGTC 1 cut(s) 2112
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 2 cut(s) 398, 2571
Psp6I CCWGG 2 cut(s) 951, 1671
PspGI CCWGG 2 cut(s) 951, 1671
PspN4I GGNNCC 7 cut(s) 400, 745, 760, 1450, 2321, 2573, 2609
PspPI GGNCC 8 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2147, 2571
PspPPI RGGWCCY 2 cut(s) 398, 2571
PstI CTGCAG 2 cut(s) 34, 44
PsuI RGATCY 3 cut(s) 88, 1530, 2056
RsaI GTAC 6 cut(s) 1837, 2475, 2584, 2609, 3028, 3100
RsaNI GTAC 6 cut(s) 1836, 2474, 2583, 2608, 3027, 3099
RseI CAYNNNNRTG 3 cut(s) 228, 2481, 2559
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 2 cut(s) 333, 2034
SatI GCNGC 9 cut(s) 30, 33, 36, 43, 803, 1431, 1904, 1982, 2097
Sau3AI GATC 8 cut(s) 88, 172, 1530, 2056, 2356, 2713, 2788, 2904
Sau96I GGNCC 8 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2147, 2571
ScaI AGTACT 2 cut(s) 2584, 3028
SchI GAGTC 5 cut(s) 781, 1073, 2359, 2504, 2792
ScrFI CCNGG 3 cut(s) 953, 1673, 2955
SduI GDGCHC 4 cut(s) 345, 1350, 2167, 2864
SfaNI GCATC 5 cut(s) 419, 441, 1442, 2216, 2395
SfcI CTRYAG 2 cut(s) 30, 40
SfuI TTCGAA 1 cut(s) 2467
SinI GGWCC 7 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2571
SmiMI CAYNNNNRTG 3 cut(s) 228, 2481, 2559
SmlI CTYRAG 3 cut(s) 1412, 1568, 2090
SmoI CTYRAG 3 cut(s) 1412, 1568, 2090
SphI GCATGC 1 cut(s) 3119
SsiI CCGC 5 cut(s) 191, 213, 612, 1293, 2697
SspI AATATT 3 cut(s) 136, 657, 901
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 3 cut(s) 951, 1671, 2953
StyI CCWWGG 3 cut(s) 1351, 2158, 2218
TaaI ACNGT 8 cut(s) 777, 1193, 1256, 1327, 1422, 1588, 1891, 3038
TaiI ACGT 4 cut(s) 869, 1654, 2407, 2782
TaqI TCGA 2 cut(s) 413, 2467
TaqII GACCGA 1 cut(s) 1397
TatI WGTACW 4 cut(s) 2473, 2582, 3026, 3098
TfiI GAWTC 5 cut(s) 269, 619, 908, 2295, 2456
TscAI CASTG 7 cut(s) 12, 187, 688, 780, 1210, 1870, 2026
TseFI GTSAC 4 cut(s) 3, 1372, 2236, 2932
TseI GCWGC 9 cut(s) 29, 32, 35, 42, 802, 1430, 1903, 1981, 2096
Tsp45I GTSAC 4 cut(s) 3, 1372, 2236, 2932
TspGWI ACGGA 1 cut(s) 1720
TspRI CASTG 7 cut(s) 12, 187, 688, 780, 1210, 1870, 2026
Vha464I CTTAAG 1 cut(s) 1412
VneI GTGCAC 1 cut(s) 2860
VpaK11BI GGWCC 7 cut(s) 398, 1298, 1355, 1449, 1844, 2063, 2571
XapI RAATTY 6 cut(s) 1598, 1877, 2284, 2707, 2813, 3011
XbaI TCTAGA 3 cut(s) 871, 2683, 2801
XceI RCATGY 1 cut(s) 3119
XcmI CCANNNNNNNNNTGG 1 cut(s) 3011
XmiI GTMKAC 6 cut(s) 1210, 1466, 1583, 1749, 1822, 2796
XmnI GAANNNNTTC 1 cut(s) 1498
ZraI GACGTC 1 cut(s) 867
ZrmI AGTACT 2 cut(s) 2584, 3028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.