Rorug01G0294400

of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0019246
Physical Location & Seq
Forward (+)
41011013 .. 41011372
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0294400.1

Sequence Viewer

Length: 360 bp
ATGGCTGGAGCTGTCGTTTCAATGGTGATCCATGGGCTCAGGCCGCTCGGAAGCTTGATGATTCAGCAAGCTAAGTTCTTCAATGAAGTCGGCGATCAAGTTGAGCTTGCACAAAGCGAGCTACTCCTGATGCGGAGCTTCCTGAAAGATGCAGATGCACGACAAGAAGATGATGAAGTGGTACGCTTCTGGGTTAATGTGGTTAGAGAAGCTGCTTATGATTTGGAGGATGCCATTGAATCTTTTGTCTTGAAAGTAGCTTTGAAGAAGGAAGCAAGTGTGAAGGTAGTTCTCAAAAGGTTTGCTTGTTTTTTGAGCAAAGGAGTGGATCGTTACAAGATCGATCGGTTTGGAAATTGA

Protein Analysis

119

Amino Acids

13.47

Weight (kDa)

5.85

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 94 9.1e-22 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g09142
rosa_multiflora Rmu_sc0006328.1_g000004
rosa_rugosa Rorug01G0294400 Rorug01G0294500
rosa_samantha Rh1AG303800 Rh1AG304000 Rh1AG304300 Rh1CG285200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 46
AciI CCGC 2 cut(s) 44, 133
AclWI GGATC 2 cut(s) 22, 336
AfaI GTAC 1 cut(s) 183
AgsI TTSAA 5 cut(s) 21, 82, 239, 253, 265
AluBI AGCT 8 cut(s) 11, 54, 71, 106, 121, 138, 212, 260
AluI AGCT 8 cut(s) 11, 54, 71, 106, 121, 138, 212, 260
AlwI GGATC 2 cut(s) 22, 336
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 1 cut(s) 212
AsuHPI GGTGA 1 cut(s) 37
BanII GRGCYC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 199
BisI GCNGC 2 cut(s) 44, 213
BlsI GCNGC 2 cut(s) 45, 214
BmsI GCATC 4 cut(s) 120, 139, 145, 220
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 31
BsaXI ACNNNNNCTCC 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 199
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 346
BsnI GGCC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 5 cut(s) 27, 94, 328, 339, 343
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 2 cut(s) 44, 133
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 51
BspDI ATCGAT 1 cut(s) 342
BspPI GGATC 2 cut(s) 22, 336
BsrBI CCGCTC 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 5 cut(s) 27, 94, 328, 339, 343
BssT1I CCWWGG 1 cut(s) 31
BstC8I GCNNGC 3 cut(s) 69, 108, 119
BstDEI CTNAG 2 cut(s) 38, 72
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 5 cut(s) 30, 97, 331, 342, 346
BstMBI GATC 5 cut(s) 27, 94, 328, 339, 343
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 342
BsuRI GGCC 1 cut(s) 43
BsuTUI ATCGAT 1 cut(s) 342
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 69, 108, 119
ClaI ATCGAT 1 cut(s) 342
Csp6I GTAC 1 cut(s) 182
CviAII CATG 1 cut(s) 32
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 38, 72
DpnI GATC 5 cut(s) 29, 96, 330, 341, 345
DpnII GATC 5 cut(s) 27, 94, 328, 339, 343
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 1 cut(s) 35
FaiI YATR 2 cut(s) 33, 219
FalI AAGNNNNNCTT 4 cut(s) 90, 122, 289, 321
FatI CATG 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 44, 213
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 44, 213
GluI GCNGC 2 cut(s) 44, 213
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 43
Hin1II CATG 1 cut(s) 35
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 2 cut(s) 61, 239
HphI GGTGA 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 50
Hpy188III TCNNGA 3 cut(s) 127, 142, 250
HpyAV CCTTC 2 cut(s) 262, 277
HpyCH4V TGCA 3 cut(s) 110, 152, 158
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 2 cut(s) 38, 72
Hsp92II CATG 1 cut(s) 35
Kzo9I GATC 5 cut(s) 27, 94, 328, 339, 343
LmnI GCTCC 2 cut(s) 8, 135
LpnPI CCDG 4 cut(s) 25, 140, 155, 175
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 4 cut(s) 120, 139, 145, 220
MaeIII GTNAC 1 cut(s) 332
MalI GATC 5 cut(s) 29, 96, 330, 341, 345
MbiI CCGCTC 1 cut(s) 46
MboI GATC 5 cut(s) 27, 94, 328, 339, 343
MboII GAAGA 3 cut(s) 70, 179, 277
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 355
MnlI CCTC 1 cut(s) 220
MseI TTAA 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 43
NcoI CCATGG 1 cut(s) 31
NdeII GATC 5 cut(s) 27, 94, 328, 339, 343
NlaIII CATG 1 cut(s) 35
PfeI GAWTC 2 cut(s) 61, 239
PkrI GCNGC 2 cut(s) 45, 214
Ple19I CGATCG 1 cut(s) 346
PvuI CGATCG 1 cut(s) 346
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 2 cut(s) 44, 213
Sau3AI GATC 5 cut(s) 27, 94, 328, 339, 343
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 4 cut(s) 120, 139, 145, 220
Sse9I AATT 1 cut(s) 355
SsiI CCGC 2 cut(s) 44, 133
StyI CCWWGG 1 cut(s) 31
TaqI TCGA 1 cut(s) 342
TasI AATT 1 cut(s) 355
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 2 cut(s) 61, 239
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 99, 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.