Rh1AG304300

of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0019246
Physical Location & Seq
Reverse (-)
53662798 .. 53666157
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG304300.1

Sequence Viewer

Length: 927 bp
ATGGAAAACGCGAAGAGCACAAGCACGGGAATCAAACGAGAGACGGAAAATGTTGTGGAGGCAGCTGATTTGGCAAAATTTTCATCATCTGAAGACAAGGCGCATTCTAACATTTCTGCCCCAGCATCTGATCATGGTTACTTGGCAAATATACTAGCACTTGAGGGTTCAAAGGATGTGCCTGTTGGCCAACCAATTGCAGTAACAGTTGATGGTGTCACTGTTGGTTCAAGTATCATAAAGATCAGCCGTGCAGCTAAGTTGCTAATTCGAGAATATGGATTAGACACATCCTCATTGAGGGCTTCAGGTGCTCATGGTACATTACTTAAAGGCGATGTTTTAGCAGCTATCAAGTCAGGAGTTGGATCTTCGAAAGGACCTCTGAATGAACCTCTTGCTGGAAGGGACCTCAGGAAAGATGATCATTGGGTTGCTGATAAAAGGAGGTTCAAAATCAACATTGATGCTACCTTTACACACTTGGATAACAAGTGTGGCATTGCGGTTCTGGCGAGGAATAGTCGTGCAAGGATGAGGGGAGTTGATTACAGAGTCACTTATGCTTCCTCAGCCTTAGAGGCTGAGGCAAAGGCTGCCTTACTAGCCGTGCAAATGGCAGAAAAGCTTAAGCTTCAAGATTACATTGTAGAGACTACCAGTCGTACTTTGAAGCGGCATATTGAGGAGAAGAATATGAGGGACAACTGTCCAGGATTCTTAGGCGGGAAGCTTAACTTTCTAAGTGAACAGAGGCAGATTTTCTGGTCTAGAGTTGATCTCAAGGCAAATCAAGCTGTGAGATTTGTGGCTCGTGCTATTCTGGAAGAAAAAATAGATGCCAGTCCCTCTAAGTTGATGCAGAATGTCCCATCTCCCCTAGTCAGATTGCTTTCGGCAGATGAAGCATTGGCCTTTGAAGAGTAA

Protein Analysis

308

Amino Acids

33.48

Weight (kDa)

9.07

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 154 - 267 1.6e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g09142
rosa_multiflora Rmu_sc0006328.1_g000004
rosa_rugosa Rorug01G0294400 Rorug01G0294500
rosa_samantha Rh1AG303800 Rh1AG304000 Rh1AG304300 Rh1CG285200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 11
AciI CCGC 3 cut(s) 506, 676, 726
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 2 cut(s) 111, 291
AfaI GTAC 2 cut(s) 322, 667
AfiI CCNNNNNNNGG 2 cut(s) 300, 401
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 6 cut(s) 171, 231, 454, 638, 673, 920
AhdI GACNNNNNGTC 1 cut(s) 660
AjnI CCWGG 1 cut(s) 712
AluBI AGCT 7 cut(s) 65, 257, 350, 628, 634, 733, 797
AluI AGCT 7 cut(s) 65, 257, 350, 628, 634, 733, 797
Alw21I GWGCWC 2 cut(s) 20, 316
Alw26I GTCTC 2 cut(s) 35, 647
AlwI GGATC 1 cut(s) 376
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 2 cut(s) 187, 912
ApeKI GCWGC 4 cut(s) 62, 254, 347, 596
ApoI RAATTY 1 cut(s) 77
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 380, 409
AsuII TTCGAA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 380, 409
AxyI CCTNAGG 1 cut(s) 413
BalI TGGCCA 1 cut(s) 189
BauI CACGAG 1 cut(s) 813
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 2 cut(s) 20, 316
BbvCI CCTCAGC 2 cut(s) 571, 585
BbvI GCAGC 4 cut(s) 74, 266, 359, 583
BccI CCATC 2 cut(s) 206, 880
BceAI ACGGC 2 cut(s) 234, 593
BciT130I CCWGG 1 cut(s) 714
BclI TGATCA 2 cut(s) 130, 424
BcoDI GTCTC 2 cut(s) 35, 647
BfaI CTAG 4 cut(s) 155, 605, 771, 881
BfrI CTTAAG 1 cut(s) 629
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 5 cut(s) 63, 255, 348, 597, 677
BlsI GCNGC 5 cut(s) 64, 256, 349, 598, 678
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 2 cut(s) 380, 409
BmeRI GACNNNNNGTC 1 cut(s) 660
BmgT120I GGNCC 2 cut(s) 380, 409
BmiI GGNNCC 1 cut(s) 410
BmrFI CCNGG 1 cut(s) 714
BmsI GCATC 4 cut(s) 134, 457, 829, 849
BoxI GACNNNNGTC 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 765, 797
Bpu10I CCTNAGC 2 cut(s) 571, 585
Bpu14I TTCGAA 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 182, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 300, 401
Bse1I ACTGG 2 cut(s) 660, 843
Bse21I CCTNAGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 714
BseGI GGATG 3 cut(s) 181, 290, 540
BseLI CCNNNNNNNGG 2 cut(s) 300, 401
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 427, 576, 585
BseNI ACTGG 2 cut(s) 660, 843
BseRI GAGGAG 1 cut(s) 701
BseXI GCAGC 4 cut(s) 74, 266, 359, 583
BseYI CCCAGC 1 cut(s) 121
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 189, 914
BsiHKAI GWGCWC 2 cut(s) 20, 316
BslFI GGGAC 4 cut(s) 422, 716, 831, 854
BslI CCNNNNNNNGG 2 cut(s) 300, 401
BsmAI GTCTC 2 cut(s) 35, 647
BsmBI CGTCTC 1 cut(s) 35
BsmFI GGGAC 4 cut(s) 422, 716, 831, 854
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 2 cut(s) 189, 914
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 20, 316
Bsp143I GATC 5 cut(s) 130, 243, 368, 424, 778
BspACI CCGC 3 cut(s) 506, 676, 726
BspANI GGCC 2 cut(s) 189, 914
BspCNI CTCAG 3 cut(s) 426, 577, 584
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 410
BspPI GGATC 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 1 cut(s) 374
BspTI CTTAAG 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 2 cut(s) 660, 843
BssMI GATC 5 cut(s) 130, 243, 368, 424, 778
BssSI CACGAG 1 cut(s) 813
Bst2BI CACGAG 1 cut(s) 813
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 3 cut(s) 208, 223, 710
Bst6I CTCTTC 2 cut(s) 8, 915
BstAFI CTTAAG 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 374
BstDEI CTNAG 8 cut(s) 258, 413, 571, 577, 585, 721, 743, 852
BstENI CCTNNNNNAGG 1 cut(s) 298
BstF5I GGATG 3 cut(s) 181, 290, 540
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 5 cut(s) 133, 246, 371, 427, 781
BstMAI GTCTC 2 cut(s) 35, 647
BstMBI GATC 5 cut(s) 130, 243, 368, 424, 778
BstMWI GCNNNNNNNGC 9 cut(s) 71, 311, 512, 572, 581, 596, 605, 794, 905
BstNI CCWGG 1 cut(s) 714
BstPAI GACNNNNGTC 1 cut(s) 708
BstSCI CCNGG 1 cut(s) 712
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 4 cut(s) 74, 266, 359, 583
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
Bsu36I CCTNAGG 1 cut(s) 413
BsuRI GGCC 2 cut(s) 189, 914
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 3 cut(s) 181, 290, 540
BtsIMutI CAGTG 1 cut(s) 219
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 380, 409
Csp6I GTAC 2 cut(s) 321, 666
CviAII CATG 2 cut(s) 134, 317
CviQI GTAC 2 cut(s) 321, 666
DdeI CTNAG 8 cut(s) 258, 413, 571, 577, 585, 721, 743, 852
DpnI GATC 5 cut(s) 132, 245, 370, 426, 780
DpnII GATC 5 cut(s) 130, 243, 368, 424, 778
DriI GACNNNNNGTC 1 cut(s) 660
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 8, 915
Eam1105I GACNNNNNGTC 1 cut(s) 660
EarI CTCTTC 2 cut(s) 8, 915
Eco47I GGWCC 2 cut(s) 380, 409
Eco57I CTGAAG 2 cut(s) 111, 291
Eco81I CCTNAGG 1 cut(s) 413
EcoNI CCTNNNNNAGG 1 cut(s) 298
EcoO109I RGGNCCY 2 cut(s) 380, 409
EcoRII CCWGG 1 cut(s) 712
Esp3I CGTCTC 1 cut(s) 35
FaeI CATG 2 cut(s) 137, 320
FaiI YATR 8 cut(s) 135, 152, 239, 279, 318, 564, 681, 698
FalI AAGNNNNNCTT 4 cut(s) 584, 616, 722, 754
FaqI GGGAC 4 cut(s) 422, 716, 831, 854
FatI CATG 2 cut(s) 133, 316
FauI CCCGC 1 cut(s) 719
FbaI TGATCA 2 cut(s) 130, 424
Fnu4HI GCNGC 5 cut(s) 63, 255, 348, 597, 677
FokI GGATG 3 cut(s) 188, 277, 547
Fsp4HI GCNGC 5 cut(s) 63, 255, 348, 597, 677
FspBI CTAG 4 cut(s) 155, 605, 771, 881
GlaI GCGC 1 cut(s) 102
GluI GCNGC 5 cut(s) 63, 255, 348, 597, 677
GsaI CCCAGC 1 cut(s) 125
HaeIII GGCC 2 cut(s) 189, 914
HhaI GCGC 1 cut(s) 103
Hin1II CATG 2 cut(s) 137, 320
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HindIII AAGCTT 3 cut(s) 626, 632, 731
HinfI GANTC 3 cut(s) 30, 555, 717
Hpy166II GTNNAC 1 cut(s) 749
Hpy188I TCNGA 4 cut(s) 91, 130, 387, 887
Hpy188III TCNNGA 6 cut(s) 272, 360, 415, 638, 771, 824
Hpy8I GTNNAC 1 cut(s) 749
HpyAV CCTTC 1 cut(s) 399
HpyCH4III ACNGT 3 cut(s) 208, 223, 710
HpyCH4V TGCA 5 cut(s) 200, 254, 530, 613, 862
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 311, 512, 572, 581, 596, 605, 794, 905
HpyF3I CTNAG 8 cut(s) 258, 413, 571, 577, 585, 721, 743, 852
Hsp92II CATG 2 cut(s) 137, 320
HspAI GCGC 1 cut(s) 101
Ksp22I TGATCA 2 cut(s) 130, 424
Kzo9I GATC 5 cut(s) 130, 243, 368, 424, 778
LguI GCTCTTC 1 cut(s) 8
Lsp1109I GCAGC 4 cut(s) 74, 266, 359, 583
LweI GCATC 4 cut(s) 134, 457, 829, 849
MaeI CTAG 4 cut(s) 155, 605, 771, 881
MaeIII GTNAC 4 cut(s) 137, 202, 217, 556
MalI GATC 5 cut(s) 132, 245, 370, 426, 780
MboI GATC 5 cut(s) 130, 243, 368, 424, 778
MboII GAAGA 5 cut(s) 25, 104, 363, 703, 839
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 2 cut(s) 20, 316
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 3 cut(s) 77, 195, 267
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 1 cut(s) 564
MmeI TCCRAC 1 cut(s) 346
Mox20I TGGCCA 1 cut(s) 189
MscI TGGCCA 1 cut(s) 189
MseI TTAA 3 cut(s) 330, 630, 735
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 65
MspCI CTTAAG 1 cut(s) 629
MspR9I CCNGG 1 cut(s) 714
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 9 cut(s) 71, 311, 512, 572, 581, 596, 605, 794, 905
NdeII GATC 5 cut(s) 130, 243, 368, 424, 778
NlaIII CATG 2 cut(s) 137, 320
NlaIV GGNNCC 1 cut(s) 410
NmuCI GTSAC 2 cut(s) 217, 556
NspV TTCGAA 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 1 cut(s) 103
PfeI GAWTC 2 cut(s) 30, 717
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 5 cut(s) 64, 256, 349, 598, 678
PleI GAGTC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 563
PpuMI RGGWCCY 2 cut(s) 380, 409
PshAI GACNNNNGTC 1 cut(s) 708
Psp5II RGGWCCY 2 cut(s) 380, 409
Psp6I CCWGG 1 cut(s) 712
PspFI CCCAGC 1 cut(s) 121
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 1 cut(s) 410
PspPI GGNCC 2 cut(s) 380, 409
PspPPI RGGWCCY 2 cut(s) 380, 409
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 1 cut(s) 368
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 2 cut(s) 322, 667
RsaNI GTAC 2 cut(s) 321, 666
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 330, 630, 735
SatI GCNGC 5 cut(s) 63, 255, 348, 597, 677
Sau3AI GATC 5 cut(s) 130, 243, 368, 424, 778
Sau96I GGNCC 2 cut(s) 380, 409
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 2 cut(s) 20, 316
SfaNI GCATC 4 cut(s) 134, 457, 829, 849
SfuI TTCGAA 1 cut(s) 374
SinI GGWCC 2 cut(s) 380, 409
SmlI CTYRAG 3 cut(s) 161, 629, 782
SmoI CTYRAG 3 cut(s) 161, 629, 782
Sse9I AATT 3 cut(s) 77, 195, 267
SsiI CCGC 3 cut(s) 506, 676, 726
SspMI CTAG 4 cut(s) 155, 605, 771, 881
StyD4I CCNGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 208, 223, 710
TaqI TCGA 2 cut(s) 271, 374
TasI AATT 3 cut(s) 77, 195, 267
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 2 cut(s) 30, 717
Tru1I TTAA 3 cut(s) 330, 630, 735
Tru9I TTAA 3 cut(s) 330, 630, 735
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 2 cut(s) 217, 556
TseI GCWGC 4 cut(s) 62, 254, 347, 596
Tsp45I GTSAC 2 cut(s) 217, 556
TspDTI ATGAA 3 cut(s) 72, 405, 918
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 1 cut(s) 226
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 380, 409
XagI CCTNNNNNAGG 1 cut(s) 298
XapI RAATTY 1 cut(s) 77
XbaI TCTAGA 1 cut(s) 770
XspI CTAG 4 cut(s) 155, 605, 771, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.