Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0018078

Physical Location & Seq

Genomic Coordinates Forward (+)

45254879 .. 45260957

Gene Structure 6079 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0334400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6012 bp

ATGCCTCGGCAGGTTGATGAGTATCTGCTTCAAAACTACAGTCATAAGCATCCATTAATATTGAAGACACAATATAATCATCTACTAGCTGTTTCATGTGAGGCATGTGGAGATCCAGTGCAAGGTCCTTACTATAGTTGCATCAAGTGTTCCAACGAATTCAATCTCCATAAGTCATGTGCCCAGCTACCAAAGGAGATCCCCCAACACCCTTTGCACAGAGAGCACCCACTTACAGTTGTGACAGAGAGAAAACTAGTCGAAAAGACGGAAAAGTTAAAGCAAGTCTTCCAGCACTCTTTGCACAGCAAGCACCCACTACGTGAACTTTACTCAAGTAGTCAAAGAGGCTTCGTGAGGTTTGAATGTCATGCTTGTAGGGCACGAATTAATGGCTTCTCCTATAGTTGTCGCCCATGTGAGTTCGACCTTCACGTCAAATGTGCTTTCAATTCCAGAAACACAGACAACATACAACATTTCATTCACAACCATCTATTGACCTTGAGTATTGAAAAAGGACCACAACTCTTTTGTACTAGATGCTTAGGGAAAATCCTTGGTCCTAGATACATTTGCGAAATTCCTTATGCGAGGGAGGGAAGTTGCTCCTCCATTGTTCTGCATCAATCTTGTGCGAAGCTAGATGAAAGGATCCAGCACCTGATGCACAAGCAACACCCACTTATTCTCATTCTAAATGACGCACAAGAATCCCACTGTGATGCTTGTGACAAAGATTGCAGCCGCAGATTCACTTACAGTTGTGTACATTGTCACTTTAACCTCCACCTCAAATGTATTCCTGAAAAAGGCTTCAAACATTTCAGTCACGAGCATCCATTAATGTTCAAGGAGCTGGAAAAGTCAGCTCCATCAATTATTTGTGATGGTTGCCAGGACCTAGTATTGGGCCCAAGGTACACTTGCATCAATACCTACAAAGGTCACAAATGCAGTTTCAATCTCCACAAGTCATGTGCAGACTTAGCCCACGAGATGCAGCACCCAATGCACCAGCAACACCCACTTATTCTCAATCTAAATGGAGCACGAGAATCCCACTGTGTTATTTGTGACAAAGATTGCAGCCGCAGATTCACTTACAATTGTGTCCAGTGTGACTTTAACCTCGACCTCAAATGTATTCCTGAAAAAGGCTTCAAACATTTCAGCCACGAGCATCCATTATTATTCAAGGAGTTGGAAGAGTCTGCTTCATTAGTTATTTGTGACGGGTGCCAAGACCCAGTATTGGGCCCAAGGTACACTTGCATCAATACCTACCAAGGTTGCAAATGTTGTTTCAATCTCCACAAGTCATGTGCAAACTTACCCCGCGAGATGCAGCACCCGATGCACCAGCAACACCCACTTATTCTTAATCTAAATGAAGCACGAGAATCCCATTGTGATGTTTGTGACAAAGATTGCAGCCGCAGATTCACTTACAGTTGTGTCCAGTGTGACTTCCACCTCGACCTCAAATGTTTTCCTAAAAAAGGCTTCAAACATTTCAGTCACGAGCATCCACAAGTGTTCAAGGAGCTGGAAGAGTCTGATCCATCAGTAATTTGTGATGGGTGCCAAGACCCAATATTGGGCTCAAGGTATACTTGCATCAATACCTACCAAGGTTGCAAATGCTGTTTCAATCTCCACAAGTCATGTGCAGACTTACCCCGTGAGATGCAGCATTCGATGCACCAACAACACCCACTTATTCTCAATCTAAATGGAGCACGAGAATCCCACTGTGATGTTTGTGACAAAGATTTAAGCCGTAGATTTACTTACAGTTGTGTCCAGTGTGACTTCAACCTCCACCCCAAATGTTTTCCTGAAAAAGGTTTCAAACATTTGAGTCACGAGCACCCATTAGTGTTCAAGGAGTTCGAAGAGTCTGCTCTATTAGTTATTTGTGATGGGTGCCAGGACCCAGTATTGGGCCCAAGGTACACTTGCATCAATACCTACCAGGGTCGCAAATGTTGTTTCAATCTCCACAAGTCATGTGCAGACTTAGCCCACGAGTTGCAGCACTCGATGCACCACCAACACCCACTTATTCTCAATCTAAATAGAGCACGAGAATCCTACTGTGATATTTGTGACAAAGATTGCAGGCCCAGATTCACTTACAGTTGTGTCCAGTGTGACTTTAACCTCGACCTTAAATATATTCCAGAAAGAGGCTTCAAACATTTCAGCCACGAGCATCCATTAGTGTTCAAGGAGCTAGAAGACTCTGCTCCATCAGTTATTTGTGATGGGTGCCAGGACCCAGTATTGGGCTCAAGGTACACTTGCATCAATACCTACCAAGGTTGTAAATGCTGTTTCAATCTCCACAAGTCATGTGCAGACTTACCCTGCGAGATGCAGCACCCGATGCACCAACAACACCCACTTATTCTCAATCTAAATGGAGCACAAGAATCCTACTGTGATGTTTGTGACAAAGATTGCAGCCGCAGATTCACTTACAGTTGTGTCCAGTGTAACTTCAACCTCGATCTCAAATGTATTCCTGAAAAAGGCTGCAAACATTTCATCCACGAGCATCCATTAGTGTTCAAGGAGTTGGAAGACTCTGCTCCATCAGTTATTTGTGATGGGTGCCAGGACCCAGTATTGGGCCCAAGGTATACTTGCATCAATACCTACCAAGGTTGCAAATGTTGTTTCAATCTCCACAAGTCATGTGCAGACTTACCCAGTAAGATGCATCACATGATGCACCAGCAACACCCACTTATTCTAAATCTAAATGGAGCACAAGAATCCTACTGTGATGTTTGTGATAAAGATTGCAACCGCAGATTCACTTACAGTTGTGTCCAGTGTGACTTCAACCTCGACCTCAAATGTATTCCTGAAAAAGGCTTCGAACATTTCAGTCATGGGCATCCACTAGTGTTCAAGGAGCTGGTAGAGTCTGCTCCATCAGTTATTTATGATGGGTGCCAGGACCCAGTATTGGGCCCAAGGTATACTTGCATCAACACCTACCAAGGTCGCAAGTGTTGTTTCAATCTCCACAAGTCATGTGCAGACTTACCCTGCGAGATGCAGCATCCGATGCACCAGCAACACCCACTTATTCTCAATCTAAATGGAGCACGAGAATCCCATTGTGATGTTTGTGACAAAGATTGCAGCCACAGATTCATTTACAGTTGTGTCCAGTGTGACTTCAACCTCGACTTCAAATGTTTTCCTAAAAAAGGCTTCAAACATTTGAGTCACGAGCATCCACTAGTGTTCAAGGAGTTGGAAGAGTCTGCTCTATCCGTTATTTGTGATGGGTGCCAGGACCCAGTATTGGGCCCAAGGTACACTTGCATCAATACCTACCAAGGTTGCAAATGTTGTTTCAATCTCCACAAGTCATGTGCAGACTTACCCCGCGAGATGCAGCACCCGATGCACCCCCAACACCCACTTATTCTCAATCTAACTGGAGCAGGAGAATCCTATTGTGATGTTTGTGACAAAGATTGCATCCGCAGATTTACTTACAGTTGTGTCCAGTGTGACTTCAACCTCGACCTCAAATGTATTCCTGAAAAATGCTTCAAACATTTCAGTCACGAGCATCCATTAGTGTTCAAGGAGTTGGAAGAGTCTCCTCTATCAGTTATTTGTGATGGGTGCCAGGACCCAGTATTGGGCCCAAGGTACACTTGCATCAATACCTACCAAGGTCACAAATGTTGTTTCAATCTCCACAAATCATGTGCAAACTTGGCCCGCGAGATGCAGCACCCAATGCACCAACAACACCCACTTATTCTCAATATAAATGGAGCACGAGAATCCCACTGTGATGTTTGTGACAAAGATTGTAGCCGCAGATTCACATACAGTTGTGTCTCGTGTGACTTCAACCTCGACCTCAAATGTATTCCTGAAAAAGGCTTCAAACATTTCAGTCACGAGCATCCATTAATGGTCAAGGAGCTGGAAGAGTCTGCTTCATCAGTTATTTGTGATGGGTGCCAGGACCGAGTATTGGGCCCTAGGTACACTTGCATCAATACCTACCGTGGTCGCAAATGCTGTTTCAATCTCCATGAGTCATGTGCAGACTTACCCTGCGAGATGCAGCACCCGATGCATCGTCTACACCCACTTATTCTCAATCTAAATGGAGCACGAGAATCCCGCTGTGATGCTTGTGACAAAGATTGCAGCCGCAGATTCACTTACAGTTGTTTTCATTGTGACTTCAACCTCGACCTCAAATGTGCTTCCGAGAAAGGCTTCTTACATTTCAGTCACGAGCATCCATTAATGATCAAGAAGCAAGGAGACAGAAACAGAGAGTGTGGTCCATTAGTTCTTTGTGATGGGTGCCAGGACCCCGTATTTGACCCTAGTTACACTTGCATCAATACGTACCGACGTCGCAAATGCCGTTTTAATCTCCACAAGTCATGTGCAGAGTTACCCGGCGAGATCCAGCACCCAGCGCACCGTCAACACCCACTTTTTCTGCTCAATAAAATTCAAGATATCAAAGTTCGTTGCTCATGTAATGCCTGCAATCAACCTTGCAGATACCGGTACAGCTGCTCCAAATGTGATTTCAACTTTCACCTCAAATGTGCCACCAATTGGCGAAACATTATCGAAACTGAATCTCATGAGCATCAGTTGATGGTCCCCCGGAACCAGCTCCACGTTATATGCGACGTTTGTGGGGAGTTCCGGAGTGGCACTACCTACTACGTCTGTAGCATCTGCCAGCTCTTGGTCGATGAGGAATGTGCTTCATTACCATGCGGCACAAAAATACCCGGGCACCAACACCGCCTCAAGCTAACCTGGTTCCTTGAAGACATTTACCCCAAGGACCAGGTTTGTAAAATCTGCTCTGCGAATATTGATAGGTGTCGTGCTGTATATTATTGCCAGGAATGTTGCGGATATGTTGCTCACACTACATGCATGCCAGTTGAGTACTTCCAGAAAGAAGACAACAAAGACAGAGACGTTAATATTGAAGATGATGATTATGAGTCTGATGATGCTGAGAAGATCAAACACTTCAGTCATCAACATCTTTTAGTCGCTAATGATCATTGTCAGGAGGTTAAGGATGACAGAATAATTACTTGTCAGGGTTGCATCCGACCTGTCACTCAAGACTTCTACAGCTGTAATAAACAAGAAGAGTCTTGCCATTTCTATCTCCACAAAACTTGCGCTCGATTGCCAGAAAAGATGCTTCTCCCTTTTCTTCACCAACACCAGTTCATACTCCACTCTCGGGCTACTTCTTTTGACGGCGTGTTTCAGTGTTATGTGTGTGGTATCCATCACCAAGGCTTTGTATACAGTTGTGATAAATGTGCTCTGCCAACCGGCGAAAACCTTTTCTACCTTGACCTTGAGTGCAGTATTTATTGGGAGAACAAATATCTTAAACATGAGTCTCATGCTCACCGCCTCCTCCTCGACATAGGATGGGAGGACTTCTATTGCAGGGGTTGTGGTTCAAAAATATTTTTCTGCTTCAGTTGTAAGAGATGCAATTTTCATCTTTGCTTAGCCTGTGTTAGATTACCTCTTACTGCTGAGCACCGATACGACACGCATCCTCTAAAGCTCACCTATGAAAGTATTGAAGACGAGCTAGGTGAATACTACTGTGAGATATGTGAAGGAAAAAGGGATCCATCACATTGGTTCTACTGGTGCAAAGAGTGCGAGTTTGACTGTCATCCCCATTGCATTAAAGGGAGGTATCCGCGAGTCCAGTTGGGGAGCACTTACAAGCACGACGCTCATGTCCAACACCCCGTCACTCTTGTTAGCAAGTGGAAGAGCCCCATTCAGGGCGATAAGAGAGACAACATTCTTCCTTGTGAGAAGTGCGGTGAACTTTGCCAAGGATTAGTATTTGAATGCGGAGAATGCAACATAAATTTCCATCGAGACGGATGTTGTGGAACCATAGCTTCGGAAAGTTCTCACAGGTATCCTCAGATCGAAGAGCTAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001871 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010208 GO:0010584 GO:0010927 GO:0016020 GO:0016043 GO:0016787 GO:0016798 GO:0022414 GO:0022607 GO:0030198 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044425 GO:0044459 GO:0044464 GO:0045229 GO:0046658 GO:0048229 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0061458 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

2003

Amino Acids

231.9

Weight (kDa)

6.64

Isoelectric Point (pI)

43.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C1_2	PF03107	14 - 61	3.1e-08	C1 domain
C1_2	PF03107	101 - 149	6.8e-09	C1 domain
C1_2	PF03107	224 - 268	8.2e-06	C1 domain
C1_2	PF03107	338 - 383	9.5e-07	C1 domain
C1_2	PF03107	453 - 497	1.3e-06	C1 domain
C1_2	PF03107	568 - 612	1.1e-08	C1 domain
C1_2	PF03107	684 - 725	3.6e-06	C1 domain
C1_2	PF03107	798 - 843	1.1e-07	C1 domain
C1_2	PF03107	914 - 958	1.3e-07	C1 domain
C1_2	PF03107	1028 - 1072	4.8e-07	C1 domain
C1_2	PF03107	1143 - 1188	5.2e-09	C1 domain
C1_2	PF03107	1258 - 1303	1.8e-07	C1 domain
C1_2	PF03107	1373 - 1418	1.1e-07	C1 domain
C1_2	PF03107	1492 - 1538	3.7e-07	C1 domain
C1_2	PF03107	1602 - 1652	1.4e-06	C1 domain
C1_2	PF03107	1864 - 1914	1.5e-07	C1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018078)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g22491
rosa_chinensis	RchiOBHm_Chr1g0367101
rosa_laevigata	RLG00000027298
rosa_multiflora	Rmu_sc0007424.1_g000005
rosa_roxburghii	Rroxscaffold_4G00289620
rosa_rugosa	Rorug01G0334400 Rorug01G0334500
rosa_samantha	Rh1BG301800 Rh1CG318100 Rh1DG334300
rosa_wichuraiana	Rw1G030340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	434, 5795
AatII	GACGTC	1 cut(s)	4411
AccB7I	CCANNNNNTGG	2 cut(s)	4620, 4756
AccI	GTMKAC	5 cut(s)	1613, 2648, 2993, 4126, 5340
AccII	CGCG	4 cut(s)	1341, 3411, 3756, 5758
AccIII	TCCGGA	1 cut(s)	4713
AclWI	GGATC	8 cut(s)	107, 193, 649, 662, 1556, 4456, 5675, 5688
AcsI	RAATTY	4 cut(s)	158, 582, 4509, 5932
AcuI	CTGAAG	2 cut(s)	5038, 5506
AcyI	GRCGYC	1 cut(s)	4408
AdeI	CACNNNGTG	1 cut(s)	323
AgeI	ACCGGT	1 cut(s)	4566
AhlI	ACTAGT	3 cut(s)	256, 2914, 3259
AjiI	CACGTC	1 cut(s)	436
AjuI	GAANNNNNNNTTGG	2 cut(s)	4574, 4606
Alw26I	GTCTC	7 cut(s)	3633, 3880, 4308, 4989, 5445, 5850, 5937
AlwI	GGATC	8 cut(s)	107, 193, 649, 662, 1556, 4456, 5675, 5688
AlwNI	CAGNNNCTG	1 cut(s)	664
Ama87I	CYCGRG	2 cut(s)	4802, 5274
Aor13HI	TCCGGA	1 cut(s)	4713
ApaI	GGGCCC	8 cut(s)	917, 1262, 1952, 2642, 2987, 3332, 3677, 4022
ApoI	RAATTY	4 cut(s)	158, 582, 4509, 5932
ArsI	GACNNNNNNTTYG	4 cut(s)	25, 57, 4355, 4387
AseI	ATTAAT	5 cut(s)	56, 390, 845, 3950, 4295
AsiGI	ACCGGT	1 cut(s)	4566
Asp700I	GAANNNNTTC	1 cut(s)	4265
AspA2I	CCTAGG	1 cut(s)	4022
AspLEI	GCGC	2 cut(s)	4477, 5213
AsuC2I	CCSGG	4 cut(s)	4455, 4672, 4803, 4804
AsuHPI	GGTGA	7 cut(s)	4592, 5240, 5318, 5441, 5608, 5657, 5897
AsuII	TTCGAA	2 cut(s)	1896, 2889
AvaI	CYCGRG	2 cut(s)	4802, 5274
AvrII	CCTAGG	1 cut(s)	4022
BamHI	GGATCC	2 cut(s)	654, 5680
BbsI	GAAGAC	7 cut(s)	71, 280, 2250, 2595, 4848, 4986, 5640
BceAI	ACGGC	3 cut(s)	1767, 4404, 5308
BcgI	CGANNNNNNTGC	2 cut(s)	1886, 1920
BciVI	GTATCC	3 cut(s)	5330, 5763, 5997
BclI	TGATCA	2 cut(s)	4299, 5083
BcnI	CCSGG	4 cut(s)	4455, 4672, 4803, 4804
BcoDI	GTCTC	7 cut(s)	3633, 3880, 4308, 4989, 5445, 5850, 5937
BcuI	ACTAGT	3 cut(s)	256, 2914, 3259
BfmI	CTRYAG	5 cut(s)	37, 133, 403, 4738, 5158
BfuI	GTATCC	3 cut(s)	5330, 5763, 5997
BlnI	CCTAGG	1 cut(s)	4022
BlpI	GCTNAGC	2 cut(s)	5554, 5583
BmcAI	AGTACT	1 cut(s)	4967
BmeT110I	CYCGRG	2 cut(s)	4802, 5274
BmgBI	CACGTC	1 cut(s)	436
BmrI	ACTGGG	8 cut(s)	1244, 1934, 2279, 2624, 2712, 2969, 3314, 3659
BmuI	ACTGGG	8 cut(s)	1244, 1934, 2279, 2624, 2712, 2969, 3314, 3659
BpiI	GAAGAC	7 cut(s)	71, 280, 2250, 2595, 4848, 4986, 5640
BpmI	CTGGAG	1 cut(s)	3483
Bpu10I	CCTNAGC	1 cut(s)	547
Bpu1102I	GCTNAGC	2 cut(s)	5554, 5583
Bpu14I	TTCGAA	2 cut(s)	1896, 2889
BpuEI	CTTGAG	7 cut(s)	319, 526, 1591, 2281, 4805, 5133, 5417
BpuMI	CCSGG	4 cut(s)	4455, 4672, 4803, 4804
BsaAI	YACGTR	2 cut(s)	323, 4401
BsaBI	GATNNNNATC	1 cut(s)	21
BsaHI	GRCGYC	1 cut(s)	4408
BsaWI	WCCGGW	2 cut(s)	4566, 4713
BsaXI	ACNNNNNCTCC	4 cut(s)	102, 132, 4562, 4592
Bse118I	RCCGGY	2 cut(s)	4566, 5369
Bse3DI	GCAATG	1 cut(s)	5734
Bse8I	GATNNNNATC	1 cut(s)	21
BseAI	TCCGGA	1 cut(s)	4713
BseJI	GATNNNNATC	1 cut(s)	21
BseMI	GCAATG	1 cut(s)	5734
BseMII	CTCAG	3 cut(s)	5028, 5574, 6005
BseRI	GAGGAG	4 cut(s)	601, 3621, 5447, 5450
BseYI	CCCAGC	2 cut(s)	183, 4471
Bsh1236I	CGCG	4 cut(s)	1341, 3411, 3756, 5758
BshTI	ACCGGT	1 cut(s)	4566
BsiHKCI	CYCGRG	2 cut(s)	4802, 5274
BsiSI	CCGG	6 cut(s)	4455, 4567, 4672, 4714, 4803, 5370
BslFI	GGGAC	1 cut(s)	4652
BsmAI	GTCTC	7 cut(s)	3633, 3880, 4308, 4989, 5445, 5850, 5937
BsmBI	CGTCTC	2 cut(s)	4989, 5937
BsmFI	GGGAC	1 cut(s)	4652
BsmI	GAATGC	3 cut(s)	1696, 5918, 5927
BsoBI	CYCGRG	2 cut(s)	4802, 5274
Bsp119I	TTCGAA	2 cut(s)	1896, 2889
Bsp120I	GGGCCC	8 cut(s)	913, 1258, 1948, 2638, 2983, 3328, 3673, 4018
Bsp13I	TCCGGA	1 cut(s)	4713
Bsp1407I	TGTACA	1 cut(s)	769
Bsp1720I	GCTNAGC	2 cut(s)	5554, 5583
BspCNI	CTCAG	3 cut(s)	5029, 5575, 6004
BspEI	TCCGGA	1 cut(s)	4713
BspFNI	CGCG	4 cut(s)	1341, 3411, 3756, 5758
BspHI	TCATGA	1 cut(s)	4648
BspPI	GGATC	8 cut(s)	107, 193, 649, 662, 1556, 4456, 5675, 5688
BspQI	GCTCTTC	2 cut(s)	5825, 5994
BspT104I	TTCGAA	2 cut(s)	1896, 2889
BsrDI	GCAATG	1 cut(s)	5734
BsrFI	RCCGGY	2 cut(s)	4566, 5369
BsrGI	TGTACA	1 cut(s)	769
BssAI	RCCGGY	2 cut(s)	4566, 5369
BssNAI	GTATAC	4 cut(s)	1614, 2649, 2994, 5341
BssNI	GRCGYC	1 cut(s)	4408
Bst1107I	GTATAC	4 cut(s)	1614, 2649, 2994, 5341
Bst6I	CTCTTC	9 cut(s)	1203, 1548, 1893, 3273, 3618, 3963, 5172, 5825, 5994
BstACI	GRCGYC	1 cut(s)	4408
BstAUI	TGTACA	1 cut(s)	769
BstBAI	YACGTR	2 cut(s)	323, 4401
BstBI	TTCGAA	2 cut(s)	1896, 2889
BstC8I	GCNNGC	6 cut(s)	311, 2126, 3754, 4546, 4751, 4955
BstDEI	CTNAG	8 cut(s)	547, 988, 2023, 5037, 5554, 5583, 5991, 6005
BstDSI	CCRYGG	1 cut(s)	4048
BstENI	CCTNNNNNAGG	8 cut(s)	810, 1155, 1500, 1845, 2535, 2880, 3225, 3915
BstFNI	CGCG	4 cut(s)	1341, 3411, 3756, 5758
BstHHI	GCGC	2 cut(s)	4477, 5213
BstMAI	GTCTC	7 cut(s)	3633, 3880, 4308, 4989, 5445, 5850, 5937
BstNSI	RCATGY	3 cut(s)	108, 4953, 4957
BstSFI	CTRYAG	5 cut(s)	37, 133, 403, 4738, 5158
BstSNI	TACGTA	1 cut(s)	4401
BstUI	CGCG	4 cut(s)	1341, 3411, 3756, 5758
BstV2I	GAAGAC	7 cut(s)	71, 280, 2250, 2595, 4848, 4986, 5640
BstX2I	RGATCY	5 cut(s)	112, 198, 654, 4461, 5680
BstXI	CCANNNNNNTGG	1 cut(s)	5691
BstYI	RGATCY	5 cut(s)	112, 198, 654, 4461, 5680
BstZ17I	GTATAC	4 cut(s)	1614, 2649, 2994, 5341
BsuI	GTATCC	3 cut(s)	5330, 5763, 5997
BtgI	CCRYGG	1 cut(s)	4048
BtrI	CACGTC	1 cut(s)	436
Cac8I	GCNNGC	6 cut(s)	311, 2126, 3754, 4546, 4751, 4955
CaiI	CAGNNNCTG	1 cut(s)	664
CciI	TCATGA	1 cut(s)	4648
CfoI	GCGC	2 cut(s)	4477, 5213
Cfr10I	RCCGGY	2 cut(s)	4566, 5369
Cfr9I	CCCGGG	1 cut(s)	4802
CseI	GACGC	2 cut(s)	713, 5798
CsiI	ACCWGGT	2 cut(s)	4829, 4860
CspAI	ACCGGT	1 cut(s)	4566
CspCI	CAANNNNNGTGG	2 cut(s)	5189, 5224
DdeI	CTNAG	8 cut(s)	547, 988, 2023, 5037, 5554, 5583, 5991, 6005
DraIII	CACNNNGTG	1 cut(s)	323
DrdI	GACNNNNNNGTC	2 cut(s)	434, 5795
DseDI	GACNNNNNNGTC	2 cut(s)	434, 5795
Eam1104I	CTCTTC	9 cut(s)	1203, 1548, 1893, 3273, 3618, 3963, 5172, 5825, 5994
EarI	CTCTTC	9 cut(s)	1203, 1548, 1893, 3273, 3618, 3963, 5172, 5825, 5994
Eco105I	TACGTA	1 cut(s)	4401
Eco32I	GATATC	1 cut(s)	4519
Eco57I	CTGAAG	2 cut(s)	5038, 5506
Eco88I	CYCGRG	2 cut(s)	4802, 5274
EcoNI	CCTNNNNNAGG	8 cut(s)	810, 1155, 1500, 1845, 2535, 2880, 3225, 3915
EcoRI	GAATTC	1 cut(s)	158
EcoRV	GATATC	1 cut(s)	4519
EcoT22I	ATGCAT	3 cut(s)	2730, 4122, 4955
Esp3I	CGTCTC	2 cut(s)	4989, 5937
FaqI	GGGAC	1 cut(s)	4652
FauI	CCCGC	4 cut(s)	1346, 3416, 3761, 4175
FbaI	TGATCA	2 cut(s)	4299, 5083
FblI	GTMKAC	5 cut(s)	1613, 2648, 2993, 4126, 5340
GlaI	GCGC	2 cut(s)	4476, 5212
GsaI	CCCAGC	2 cut(s)	187, 4475
GsuI	CTGGAG	1 cut(s)	3483
HapII	CCGG	6 cut(s)	4455, 4567, 4672, 4714, 4803, 5370
HgaI	GACGC	2 cut(s)	713, 5798
HhaI	GCGC	2 cut(s)	4477, 5213
Hin1I	GRCGYC	1 cut(s)	4408
Hin6I	GCGC	2 cut(s)	4475, 5211
HinP1I	GCGC	2 cut(s)	4475, 5211
HincII	GTYRAC	1 cut(s)	4484
HindII	GTYRAC	1 cut(s)	4484
HpaII	CCGG	6 cut(s)	4455, 4567, 4672, 4714, 4803, 5370
HphI	GGTGA	7 cut(s)	4592, 5240, 5318, 5441, 5608, 5657, 5897
Hpy188I	TCNGA	7 cut(s)	1561, 3081, 4258, 5029, 5138, 5971, 5994
Hpy99I	CGWCG	4 cut(s)	4410, 4413, 4700, 5792
HpyAV	CCTTC	2 cut(s)	440, 5663
HpyCH4IV	ACGT	8 cut(s)	322, 435, 4400, 4408, 4686, 4698, 4734, 4998
HpyF3I	CTNAG	8 cut(s)	547, 988, 2023, 5037, 5554, 5583, 5991, 6005
HpySE526I	ACGT	8 cut(s)	322, 435, 4400, 4408, 4686, 4698, 4734, 4998
Hsp92I	GRCGYC	1 cut(s)	4408
HspAI	GCGC	2 cut(s)	4475, 5211
Kpn2I	TCCGGA	1 cut(s)	4713
Ksp22I	TGATCA	2 cut(s)	4299, 5083
LguI	GCTCTTC	2 cut(s)	5825, 5994
MabI	ACCWGGT	2 cut(s)	4829, 4860
MaeII	ACGT	8 cut(s)	322, 435, 4400, 4408, 4686, 4698, 4734, 4998
MfeI	CAATTG	2 cut(s)	1108, 4618
MflI	RGATCY	5 cut(s)	112, 198, 654, 4461, 5680
MmeI	TCCRAC	7 cut(s)	177, 1185, 2565, 3255, 3600, 5161, 5824
Mph1103I	ATGCAT	3 cut(s)	2730, 4122, 4955
MroI	TCCGGA	1 cut(s)	4713
MroXI	GAANNNNTTC	1 cut(s)	4265
MslI	CAYNNNNRTG	6 cut(s)	723, 1413, 1758, 3138, 3828, 4783
MspA1I	CMGCKG	3 cut(s)	4170, 4575, 5163
MspI	CCGG	6 cut(s)	4455, 4567, 4672, 4714, 4803, 5370
MunI	CAATTG	2 cut(s)	1108, 4618
Mva1269I	GAATGC	3 cut(s)	1696, 5918, 5927
MvnI	CGCG	4 cut(s)	1341, 3411, 3756, 5758
NciI	CCSGG	4 cut(s)	4455, 4672, 4803, 4804
NsiI	ATGCAT	3 cut(s)	2730, 4122, 4955
NspI	RCATGY	3 cut(s)	108, 4953, 4957
NspV	TTCGAA	2 cut(s)	1896, 2889
PaeI	GCATGC	1 cut(s)	4957
PagI	TCATGA	1 cut(s)	4648
PciSI	GCTCTTC	2 cut(s)	5825, 5994
PcsI	WCGNNNNNNNCGW	3 cut(s)	432, 4417, 4692
PctI	GAATGC	3 cut(s)	1696, 5918, 5927
PdmI	GAANNNNTTC	1 cut(s)	4265
PflMI	CCANNNNNTGG	2 cut(s)	4620, 4756
PinAI	ACCGGT	1 cut(s)	4566
Ppu21I	YACGTR	2 cut(s)	323, 4401
PpuMI	RGGWCCY	9 cut(s)	125, 901, 1936, 2281, 2626, 2971, 3316, 3661, 4363
PshBI	ATTAAT	5 cut(s)	56, 390, 845, 3950, 4295
Psp5II	RGGWCCY	9 cut(s)	125, 901, 1936, 2281, 2626, 2971, 3316, 3661, 4363
PspFI	CCCAGC	2 cut(s)	183, 4471
PspOMI	GGGCCC	8 cut(s)	913, 1258, 1948, 2638, 2983, 3328, 3673, 4018
PspPPI	RGGWCCY	9 cut(s)	125, 901, 1936, 2281, 2626, 2971, 3316, 3661, 4363
PstNI	CAGNNNCTG	1 cut(s)	664
PsuI	RGATCY	5 cut(s)	112, 198, 654, 4461, 5680
PvuII	CAGCTG	2 cut(s)	4575, 5163
RseI	CAYNNNNRTG	6 cut(s)	723, 1413, 1758, 3138, 3828, 4783
SapI	GCTCTTC	2 cut(s)	5825, 5994
ScaI	AGTACT	1 cut(s)	4967
SexAI	ACCWGGT	2 cut(s)	4829, 4860
SfcI	CTRYAG	5 cut(s)	37, 133, 403, 4738, 5158
SfuI	TTCGAA	2 cut(s)	1896, 2889
SmaI	CCCGGG	1 cut(s)	4804
SmiMI	CAYNNNNRTG	6 cut(s)	723, 1413, 1758, 3138, 3828, 4783
SmlI	CTYRAG	7 cut(s)	334, 505, 1606, 2296, 4820, 5148, 5396
SmoI	CTYRAG	7 cut(s)	334, 505, 1606, 2296, 4820, 5148, 5396
SnaBI	TACGTA	1 cut(s)	4401
SpeI	ACTAGT	3 cut(s)	256, 2914, 3259
SphI	GCATGC	1 cut(s)	4957
SspI	AATATT	5 cut(s)	60, 1599, 4888, 5005, 5511
TaiI	ACGT	8 cut(s)	325, 438, 4403, 4411, 4689, 4701, 4737, 5001
TaqII	GACCGA	1 cut(s)	4023
TatI	WGTACW	3 cut(s)	536, 769, 4965
TauI	GCSGC	7 cut(s)	750, 1095, 1440, 2475, 3855, 4200, 4791
TspGWI	ACGGA	3 cut(s)	284, 3283, 5961
TspMI	CCCGGG	1 cut(s)	4802
Van91I	CCANNNNNTGG	2 cut(s)	4620, 4756
VspI	ATTAAT	5 cut(s)	56, 390, 845, 3950, 4295
XagI	CCTNNNNNAGG	8 cut(s)	810, 1155, 1500, 1845, 2535, 2880, 3225, 3915
XapI	RAATTY	4 cut(s)	158, 582, 4509, 5932
XceI	RCATGY	3 cut(s)	108, 4953, 4957
XmaI	CCCGGG	1 cut(s)	4802
XmaJI	CCTAGG	1 cut(s)	4022
XmiI	GTMKAC	5 cut(s)	1613, 2648, 2993, 4126, 5340
XmnI	GAANNNNTTC	1 cut(s)	4265
ZraI	GACGTC	1 cut(s)	4409
ZrmI	AGTACT	1 cut(s)	4967
Zsp2I	ATGCAT	3 cut(s)	2730, 4122, 4955

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.