Rorug01G0383900

Belongs to the glycosyl hydrolase 32 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008230
Physical Location & Seq
Reverse (-)
49590019 .. 49598699
8681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0383900.1

Sequence Viewer

Length: 1200 bp
ATGGAAAAGCGAGTGACGAGAATTGTCGAACAAGGGTTTGCGATAGGGAGAATGGGAAAGAAAAACAAAACAAGGGACAAGAAGAAGAAGGTTGCGTGGGACAGGCCCATCTACCTGTACCTACAGGTCAAGGCAGACGACAACTCGGTGTCGTGTTTATGGTTCGTCATTGAAGATGGACGGCGGCCGAGGCAAATCAGTGGATCTGAAGAGATTGATGATGATGATAATAAGGTAATCTTCCGTCCCCTAGATCTTCCCGAATTTGATGATGATTACCTGCGCCTTCCTCACGGCGTTGTGGTCTTGGACTCCCGATTATTCATGATTGGTGGATATCAATGTAGAAAGCGAGATTCCTTTGGTGTGAAATCCTATATTGGGTATGATTACTTGGACCTGAAACCCTCCTATGTAGGCAAGGAATGGCGTTCTGTACAAGGGGTTTCATTCCCTTCGCAAGAGTCGGTGGCCTGCAACAAAGACGGTCTCATCTATACCTTGGGACAGCGACGTTGTCACCGTCTCGATCCTCGCTCTGGTGTCCGATCGGAGTTTCCCGCCACATGCGGGTTTCGAACACAGCTTGGTATATCAAACAAGTACATTTACACCTATTCCCTTGGTATTTATAATCTCCTGTTTCGTTATGATTTGGAGAAACACGAGTGGGACTGCATCTCTAGCAGGTTCTGGGGTCTCTGGGGCCCTGGAGTGGTGATTTCCGGCGACTCCTACTTATTATGCTTTGGCTCTATGAAACCCACCGACAAATTCTCCGAGCAGCTTGGTGTTTACGTTTTTGATATTCACCGGCGTCAGTGGCTAGATGAACCAGTCAAGGGCCTTGATGATGCTCTTCCCGTTATCCCAGACACGTACGAGTCCTGGGAAAGGCACCTTTACCTCCACCATTTACCGCGCTTGTTCCAAATCGGAACCAAAAGATTTGCTTTCGTTTGGGAGGAACGTTTTGTTCGGGGTCAGGGTCTTCTTCATTGCCACAAGTTCACTATCGACCACATCCCCTGCAGCAGCTCCTCTCATCGAACCAACAACCAACCCACCTCCTTTGTTGCGCGAATAGTCTCAAGTGGATTCCTTACTCTGCAACGCGCCGGATTACTTGGTTGCGCTGTAGGAATTGAATCTTATTCACGAGGCACAGGAACAGTGGATCATCTGCACCTGAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

399

Amino Acids

46.09

Weight (kDa)

8.86

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
Acc36I ACCTGC 2 cut(s) 288, 678
AccB1I GGYRCC 1 cut(s) 897
AccII CGCG 3 cut(s) 922, 1081, 1116
AciI CCGC 4 cut(s) 184, 561, 570, 920
AclI AACGTT 1 cut(s) 970
AclWI GGATC 3 cut(s) 211, 524, 1185
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 2 cut(s) 263, 773
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 817
AfaI GTAC 4 cut(s) 119, 438, 605, 881
AfiI CCNNNNNNNGG 6 cut(s) 381, 539, 570, 715, 842, 894
AflIII ACRYGT 1 cut(s) 876
AgsI TTSAA 2 cut(s) 173, 1148
AjnI CCWGG 2 cut(s) 709, 887
AluBI AGCT 4 cut(s) 586, 787, 1038, 1197
AluI AGCT 4 cut(s) 586, 787, 1038, 1197
Alw26I GTCTC 4 cut(s) 494, 530, 704, 1093
AlwI GGATC 3 cut(s) 211, 524, 1185
AlwNI CAGNNNCTG 1 cut(s) 693
AoxI GGCC 5 cut(s) 104, 185, 471, 706, 844
ApaI GGGCCC 1 cut(s) 710
ApeKI GCWGC 3 cut(s) 784, 1032, 1035
ApoI RAATTY 2 cut(s) 263, 773
AspLEI GCGC 5 cut(s) 285, 924, 1081, 1118, 1136
AspS9I GGNCC 5 cut(s) 105, 397, 706, 707, 844
AsuHPI GGTGA 3 cut(s) 512, 730, 803
AsuII TTCGAA 1 cut(s) 577
AvaII GGWCC 1 cut(s) 397
BaeGI GKGCMC 1 cut(s) 710
BanI GGYRCC 1 cut(s) 897
BanII GRGCYC 1 cut(s) 710
BauI CACGAG 2 cut(s) 665, 1158
BbsI GAAGAC 1 cut(s) 983
BbvI GCAGC 3 cut(s) 796, 1044, 1047
BccI CCATC 2 cut(s) 116, 170
BceAI ACGGC 2 cut(s) 197, 310
BciT130I CCWGG 2 cut(s) 711, 889
BcoDI GTCTC 4 cut(s) 494, 530, 704, 1093
BfaI CTAG 4 cut(s) 251, 684, 827, 1198
BfmI CTRYAG 3 cut(s) 122, 1030, 1137
BfuAI ACCTGC 2 cut(s) 288, 678
BglII AGATCT 1 cut(s) 253
BisI GCNGC 4 cut(s) 185, 785, 1033, 1036
BlsI GCNGC 4 cut(s) 186, 786, 1034, 1037
Bme1390I CCNGG 2 cut(s) 711, 889
Bme18I GGWCC 1 cut(s) 397
BmgT120I GGNCC 5 cut(s) 105, 397, 706, 707, 844
BmiI GGNNCC 4 cut(s) 707, 708, 899, 940
BmrFI CCNGG 2 cut(s) 711, 889
BmsI GCATC 2 cut(s) 687, 844
BpiI GAAGAC 1 cut(s) 983
BpmI CTGGAG 1 cut(s) 732
Bpu14I TTCGAA 1 cut(s) 577
BpuEI CTTGAG 1 cut(s) 1075
BsaAI YACGTR 1 cut(s) 879
BsaHI GRCGYC 1 cut(s) 817
BsaI GGTCTC 2 cut(s) 494, 704
BsaJI CCNNGG 5 cut(s) 188, 501, 622, 709, 888
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 6 cut(s) 381, 539, 570, 715, 842, 894
Bse118I RCCGGY 1 cut(s) 813
Bse1I ACTGG 1 cut(s) 836
Bse3DI GCAATG 1 cut(s) 997
BseBI CCWGG 2 cut(s) 711, 889
BseDI CCNNGG 5 cut(s) 188, 501, 622, 709, 888
BseGI GGATG 1 cut(s) 1023
BseLI CCNNNNNNNGG 6 cut(s) 381, 539, 570, 715, 842, 894
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 1 cut(s) 1181
BseNI ACTGG 1 cut(s) 836
BseRI GAGGAG 1 cut(s) 1030
BseSI GKGCMC 1 cut(s) 710
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 3 cut(s) 796, 1044, 1047
BsgI GTGCAG 1 cut(s) 1169
Bsh1236I CGCG 3 cut(s) 922, 1081, 1116
Bsh1285I CGRYCG 2 cut(s) 188, 551
BshFI GGCC 5 cut(s) 106, 187, 473, 708, 846
BshNI GGYRCC 1 cut(s) 897
BsiEI CGRYCG 2 cut(s) 188, 551
BsiSI CCGG 3 cut(s) 726, 814, 1119
BsiWI CGTACG 1 cut(s) 879
BslFI GGGAC 5 cut(s) 89, 113, 231, 519, 686
BslI CCNNNNNNNGG 6 cut(s) 381, 539, 570, 715, 842, 894
BsmAI GTCTC 4 cut(s) 494, 530, 704, 1093
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 5 cut(s) 89, 113, 231, 519, 686
BsnI GGCC 5 cut(s) 106, 187, 473, 708, 846
Bso31I GGTCTC 2 cut(s) 494, 704
Bsp119I TTCGAA 1 cut(s) 577
Bsp120I GGGCCC 1 cut(s) 706
Bsp1286I GDGCHC 1 cut(s) 710
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 5 cut(s) 203, 253, 529, 548, 1177
BspACI CCGC 4 cut(s) 184, 561, 570, 920
BspANI GGCC 5 cut(s) 106, 187, 473, 708, 846
BspCNI CTCAG 1 cut(s) 1182
BspFNI CGCG 3 cut(s) 922, 1081, 1116
BspHI TCATGA 1 cut(s) 324
BspLI GGNNCC 4 cut(s) 707, 708, 899, 940
BspMAI CTGCAG 1 cut(s) 1034
BspMI ACCTGC 2 cut(s) 288, 678
BspPI GGATC 3 cut(s) 211, 524, 1185
BspQI GCTCTTC 1 cut(s) 864
BspT104I TTCGAA 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 897
BspTNI GGTCTC 2 cut(s) 494, 704
BsrDI GCAATG 1 cut(s) 997
BsrFI RCCGGY 1 cut(s) 813
BsrGI TGTACA 1 cut(s) 436
BsrI ACTGG 1 cut(s) 836
BssAI RCCGGY 1 cut(s) 813
BssECI CCNNGG 5 cut(s) 188, 501, 622, 709, 888
BssMI GATC 5 cut(s) 203, 253, 529, 548, 1177
BssNI GRCGYC 1 cut(s) 817
BssSI CACGAG 2 cut(s) 665, 1158
BssT1I CCWWGG 2 cut(s) 501, 622
Bst2BI CACGAG 2 cut(s) 665, 1158
Bst2UI CCWGG 2 cut(s) 711, 889
Bst4CI ACNGT 3 cut(s) 488, 524, 1174
Bst6I CTCTTC 2 cut(s) 204, 864
BstACI GRCGYC 1 cut(s) 817
BstAUI TGTACA 1 cut(s) 436
BstBAI YACGTR 1 cut(s) 879
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 475
BstDEI CTNAG 1 cut(s) 1190
BstENI CCTNNNNNAGG 1 cut(s) 892
BstF5I GGATG 1 cut(s) 1023
BstFNI CGCG 3 cut(s) 922, 1081, 1116
BstHHI GCGC 5 cut(s) 285, 924, 1081, 1118, 1136
BstKTI GATC 5 cut(s) 206, 256, 532, 551, 1180
BstMAI GTCTC 4 cut(s) 494, 530, 704, 1093
BstMBI GATC 5 cut(s) 203, 253, 529, 548, 1177
BstMCI CGRYCG 2 cut(s) 188, 551
BstMWI GCNNNNNNNGC 3 cut(s) 190, 684, 823
BstNI CCWGG 2 cut(s) 711, 889
BstNSI RCATGY 1 cut(s) 570
BstSCI CCNGG 2 cut(s) 709, 887
BstSFI CTRYAG 3 cut(s) 122, 1030, 1137
BstSLI GKGCMC 1 cut(s) 710
BstUI CGCG 3 cut(s) 922, 1081, 1116
BstV1I GCAGC 3 cut(s) 796, 1044, 1047
BstV2I GAAGAC 1 cut(s) 983
BstX2I RGATCY 2 cut(s) 203, 253
BstYI RGATCY 2 cut(s) 203, 253
BstZI CGGCCG 1 cut(s) 185
BsuRI GGCC 5 cut(s) 106, 187, 473, 708, 846
BtsCI GGATG 1 cut(s) 1023
BtsIMutI CAGTG 3 cut(s) 205, 827, 1179
BveI ACCTGC 2 cut(s) 288, 678
Cac8I GCNNGC 1 cut(s) 475
CaiI CAGNNNCTG 1 cut(s) 693
CciI TCATGA 1 cut(s) 324
CfoI GCGC 5 cut(s) 285, 924, 1081, 1118, 1136
Cfr10I RCCGGY 1 cut(s) 813
Cfr13I GGNCC 5 cut(s) 105, 397, 706, 707, 844
CseI GACGC 1 cut(s) 806
Csp6I GTAC 4 cut(s) 118, 437, 604, 880
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 2 cut(s) 325, 567
CviQI GTAC 4 cut(s) 118, 437, 604, 880
DdeI CTNAG 1 cut(s) 1190
DpnI GATC 5 cut(s) 205, 255, 531, 550, 1179
DpnII GATC 5 cut(s) 203, 253, 529, 548, 1177
EaeI YGGCCR 1 cut(s) 185
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 2 cut(s) 204, 864
EarI CTCTTC 2 cut(s) 204, 864
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 501, 622
Eco24I GRGCYC 1 cut(s) 710
Eco31I GGTCTC 2 cut(s) 494, 704
Eco32I GATATC 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 397
Eco52I CGGCCG 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoO109I RGGNCCY 3 cut(s) 706, 707, 844
EcoRII CCWGG 2 cut(s) 709, 887
EcoRV GATATC 1 cut(s) 338
EcoT14I CCWWGG 2 cut(s) 501, 622
EcoT38I GRGCYC 1 cut(s) 710
ErhI CCWWGG 2 cut(s) 501, 622
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 2 cut(s) 328, 570
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 937, 969
FaqI GGGAC 5 cut(s) 89, 113, 231, 519, 686
FatI CATG 2 cut(s) 324, 566
FauI CCCGC 2 cut(s) 563, 568
Fnu4HI GCNGC 4 cut(s) 185, 785, 1033, 1036
FokI GGATG 1 cut(s) 1010
FriOI GRGCYC 1 cut(s) 710
Fsp4HI GCNGC 4 cut(s) 185, 785, 1033, 1036
FspBI CTAG 4 cut(s) 251, 684, 827, 1198
GlaI GCGC 5 cut(s) 284, 923, 1080, 1117, 1135
GluI GCNGC 4 cut(s) 185, 785, 1033, 1036
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 5 cut(s) 106, 187, 473, 708, 846
HapII CCGG 3 cut(s) 726, 814, 1119
HgaI GACGC 1 cut(s) 806
HhaI GCGC 5 cut(s) 285, 924, 1081, 1118, 1136
Hin1I GRCGYC 1 cut(s) 817
Hin1II CATG 2 cut(s) 328, 570
Hin6I GCGC 5 cut(s) 283, 922, 1079, 1116, 1134
HinP1I GCGC 5 cut(s) 283, 922, 1079, 1116, 1134
HinfI GANTC 7 cut(s) 311, 356, 464, 731, 884, 1098, 1148
HpaII CCGG 3 cut(s) 726, 814, 1119
HphI GGTGA 3 cut(s) 512, 730, 803
Hpy166II GTNNAC 2 cut(s) 796, 1011
Hpy188I TCNGA 5 cut(s) 208, 548, 553, 781, 938
Hpy188III TCNNGA 5 cut(s) 260, 315, 325, 527, 1158
Hpy8I GTNNAC 2 cut(s) 796, 1011
Hpy99I CGWCG 1 cut(s) 516
HpyAV CCTTC 3 cut(s) 82, 296, 465
HpyCH4III ACNGT 3 cut(s) 488, 524, 1174
HpyCH4IV ACGT 4 cut(s) 514, 798, 878, 970
HpyCH4V TGCA 5 cut(s) 477, 678, 1032, 1111, 1186
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 684, 823
HpyF3I CTNAG 1 cut(s) 1190
HpySE526I ACGT 4 cut(s) 514, 798, 878, 970
Hsp92I GRCGYC 1 cut(s) 817
Hsp92II CATG 2 cut(s) 328, 570
HspAI GCGC 5 cut(s) 283, 922, 1079, 1116, 1134
Kzo9I GATC 5 cut(s) 203, 253, 529, 548, 1177
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 1 cut(s) 1043
Lsp1109I GCAGC 3 cut(s) 796, 1044, 1047
LweI GCATC 2 cut(s) 687, 844
MaeI CTAG 4 cut(s) 251, 684, 827, 1198
MaeII ACGT 4 cut(s) 514, 798, 878, 970
MaeIII GTNAC 2 cut(s) 13, 518
MalI GATC 5 cut(s) 205, 255, 531, 550, 1179
MboI GATC 5 cut(s) 203, 253, 529, 548, 1177
MboII GAAGA 9 cut(s) 94, 97, 185, 221, 232, 248, 851, 983, 986
MflI RGATCY 2 cut(s) 203, 253
MhlI GDGCHC 1 cut(s) 710
MluCI AATT 4 cut(s) 21, 263, 773, 1143
MlyI GAGTC 4 cut(s) 305, 473, 725, 893
MnlI CCTC 9 cut(s) 183, 300, 418, 543, 917, 958, 1051, 1078, 1154
MspI CCGG 3 cut(s) 726, 814, 1119
MspR9I CCNGG 2 cut(s) 711, 889
MvaI CCWGG 2 cut(s) 711, 889
MvnI CGCG 3 cut(s) 922, 1081, 1116
MwoI GCNNNNNNNGC 3 cut(s) 190, 684, 823
NdeII GATC 5 cut(s) 203, 253, 529, 548, 1177
NlaIII CATG 2 cut(s) 328, 570
NlaIV GGNNCC 4 cut(s) 707, 708, 899, 940
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 13, 518
NspI RCATGY 1 cut(s) 570
NspV TTCGAA 1 cut(s) 577
PagI TCATGA 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PfeI GAWTC 3 cut(s) 356, 1098, 1148
Pfl23II CGTACG 1 cut(s) 879
PflFI GACNNNGTC 1 cut(s) 516
PkrI GCNGC 4 cut(s) 186, 786, 1034, 1037
Ple19I CGATCG 1 cut(s) 551
PleI GAGTC 4 cut(s) 305, 472, 725, 892
PpsI GAGTC 4 cut(s) 305, 472, 725, 892
Ppu21I YACGTR 1 cut(s) 879
PsiI TTATAA 1 cut(s) 633
Psp1406I AACGTT 1 cut(s) 970
Psp6I CCWGG 2 cut(s) 709, 887
PspGI CCWGG 2 cut(s) 709, 887
PspLI CGTACG 1 cut(s) 879
PspN4I GGNNCC 4 cut(s) 707, 708, 899, 940
PspOMI GGGCCC 1 cut(s) 706
PspPI GGNCC 5 cut(s) 105, 397, 706, 707, 844
PstI CTGCAG 1 cut(s) 1034
PstNI CAGNNNCTG 1 cut(s) 693
PsuI RGATCY 2 cut(s) 203, 253
PsyI GACNNNGTC 1 cut(s) 516
PvuI CGATCG 1 cut(s) 551
RsaI GTAC 4 cut(s) 119, 438, 605, 881
RsaNI GTAC 4 cut(s) 118, 437, 604, 880
SapI GCTCTTC 1 cut(s) 864
SatI GCNGC 4 cut(s) 185, 785, 1033, 1036
Sau3AI GATC 5 cut(s) 203, 253, 529, 548, 1177
Sau96I GGNCC 5 cut(s) 105, 397, 706, 707, 844
SchI GAGTC 4 cut(s) 305, 473, 725, 893
ScrFI CCNGG 2 cut(s) 711, 889
SduI GDGCHC 1 cut(s) 710
SfaNI GCATC 2 cut(s) 687, 844
SfcI CTRYAG 3 cut(s) 122, 1030, 1137
SfuI TTCGAA 1 cut(s) 577
SgrAI CRCCGGYG 1 cut(s) 813
SinI GGWCC 1 cut(s) 397
SmlI CTYRAG 1 cut(s) 1090
SmoI CTYRAG 1 cut(s) 1090
Sse9I AATT 4 cut(s) 21, 263, 773, 1143
SsiI CCGC 4 cut(s) 184, 561, 570, 920
SspMI CTAG 4 cut(s) 251, 684, 827, 1198
StyD4I CCNGG 2 cut(s) 709, 887
StyI CCWWGG 2 cut(s) 501, 622
TaaI ACNGT 3 cut(s) 488, 524, 1174
TaiI ACGT 4 cut(s) 517, 801, 881, 973
TaqI TCGA 5 cut(s) 27, 528, 577, 1017, 1048
TasI AATT 4 cut(s) 21, 263, 773, 1143
TatI WGTACW 2 cut(s) 436, 603
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 3 cut(s) 356, 1098, 1148
TscAI CASTG 3 cut(s) 205, 827, 1179
TseFI GTSAC 2 cut(s) 13, 518
TseI GCWGC 3 cut(s) 784, 1032, 1035
Tsp45I GTSAC 2 cut(s) 13, 518
TspDTI ATGAA 5 cut(s) 313, 438, 773, 846, 986
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 3 cut(s) 205, 827, 1179
Tth111I GACNNNGTC 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 397
XagI CCTNNNNNAGG 1 cut(s) 892
XapI RAATTY 2 cut(s) 263, 773
XceI RCATGY 1 cut(s) 570
XspI CTAG 4 cut(s) 251, 684, 827, 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.