Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0007413

Physical Location & Seq

Genomic Coordinates Reverse (-)

49741810 .. 49745836

Gene Structure 4027 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0385900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGAAGATCGAAACGTGAGAATGAATACTGGAATGTCCCATGTGATGCCGTATATCAAAGACTCGAAGGACCGGGACGCCGTGTCGTTGGTGTGCCGACGGTGGTACGAGCTGGACGCGCTCACGCGCGAGCAAGTGACCATCGCGCTCTGCTACACGACGACTCCCGAGAGGCTCAGGAGGCGGTTCCGGCAGCTCAAGTCGCTGAAGCTGAAGGGGAAGCCGAGGGCGGCGATGTTTAATCTGATACCGGAGGATTGGGGAGGCTTTGTGACGCCGTGGGTAGAGGAGATTGCCGAGTCGTTTAAGAGCTTGAAGGCTTTGCATTTCCGGAGAATGATTGTGAGGGATTCCGACTTGGAGCTATTGGCGCGGTCACGCGGCCGTGAGCTCCAATCGCTTAAGCTCGACAAGTGCTCCGGGTTTTCGACTGACGGCCTTGTCCACATCGCCCGCTCCTGCAGGGATTTGAGGACCTTGTTTTTGGAGGAGAGCTCGATAATTGAGAAAGAGGATGAGTGGGGTGAATGGCTACATGAGCTTGCTACAAACAACACTGTACTCGAGACTTTAAACTTCTATATGACGGATCTTGGCAGAATCAGATTTGAAGACCTCGAACTCATAGCCAGGAATTGCCCCTCCTTAACCACTGTGAAAATTAGCGATTGTGAAATTTTAGATCTTCTGGGTTTCTTCCGTCATGCTTCTGCTTTAGAAGAATTTTGTGGAGGTTCCTTCGATGATCGATCAGAGAATGATCAATCAGAGAAGTACTCAGTTGTATCACTGCCACGAAAGATATGTCTTTTGGGTCTAACGATGTTGGGGAAGAATGAAATGCCTATTGTATTCCCATTAGCAGCTGCTCTCAAAAAGTTGGATCTCCTATATGCATTGCTCGACACTGAAGACCATTGTACTTTAATTCAAAAGTGCCCCAACTTGGAAGTTCTTGAGACAAGGAATGTCATTGGAGATAGAGGATTAGAAGTCCTTGCTCGGAGTTGCAAGAGACTGAAGAGGCTCCGAATTGAGCGAGGTGCTGATGAGCAAGGTATGGAGGACGAAGATGGTGTTGTTTCACAGAGGGGTCTGATGGCTTTGGCACAGGGCTGCCTGGAACTGGAGTACTTGGCTGTCTACGTATCAAATATCACCAATGCATCTCTGGAATACATTGGGACTCACTCTAAAAATCTAAATGATTTTCGCCTTGTCCTGCTTGACCGTGAAGAGACGATAACAGATTTACCACTTGACAATGGCGTTCGAGCTCTATTGAGGGGATGCCAAAAGCTTCGAAGGTTTGCTTTGTATCTCCGTCCTGGGGGTTTGACTGATTTGGGGCTTAGTTACGTGGGCCAGTATAGTCAAAATGTGAGATGGATGCTTCTAGGTTATGTTGGTGAATCTGATGGAGGGCTTTTGGAGTTTTCAAAGGGTTGCCCTAGTCTGCAGAAACTAGAAATGAGGGGCTGTTGCTTCAGTGAGCGTGCACTGGCTGTTGCAGTGATGCAGCTGACTGCGCTAAGGTACTTGTGGGTGCAAGGGTACAGGGGATCCCCAACGGGTCGTGATCTTTTGGCAATGGCTCGCCCGTATTGGAATATTGAGTTGATTCCTCCTAGACGAGTTGATGTTCTTGATATGATCGGGGAGGCAGTAGTGGTTGAGCATCCTGCTCATATTCTTGCATACTACTCTCTTGCTGGACCAAGAACAGATTTTCCAGATACTGTTATCCCCTTGGATCCAGTCTTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0031976 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

589

Amino Acids

67.04

Weight (kDa)

5.72

Isoelectric Point (pI)

45.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box_5	PF18511	13 - 44	2.9e-18	F-box
Transp_inhibit	PF18791	65 - 110	1.3e-22	Transport inhibitor response 1 protein domain
DUF7885	PF25372	96 - 406	8.5e-08	Leucine Rich Repeat Domain of unknown function (DUF7885)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51545
fragaria_vesca	FvH4_7g27960 FvH4_7g27960 FvH4_7g27960 FvH4_7g27960 FvH4_7g27960 FvH4_7g27960
malus_domestica	MD01G1184500.v1.1
prunus_persica	Prupe.2G280700_v2.0.a1 Prupe.2G280700_v2.0.a1 Prupe.2G280700_v2.0.a1
pyrus_communis	pycom01g19610 pycom07g22940
rosa_chinensis	RchiOBHm_Chr1g0374911
rosa_laevigata	RLG00000026707
rosa_multiflora	Rmu_co8480605.1_g000001 Rmu_sc0003145.1_g000009 Rmu_sc0004947.1_g000035
rosa_roxburghii	Rroxscaffold_4G00283180
rosa_rugosa	Rorug01G0385900
rosa_samantha	Rh1AG397400 Rh1BG361300 Rh1CG374900 Rh1DG392300
rosa_wichuraiana	Rw1G035240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	440
AccBSI	CCGCTC	1 cut(s)	456
AccI	GTMKAC	1 cut(s)	1143
AccII	CGCG	6 cut(s)	119, 127, 129, 146, 373, 381
AccIII	TCCGGA	1 cut(s)	330
AciI	CCGC	5 cut(s)	184, 230, 373, 381, 454
AclWI	GGATC	6 cut(s)	597, 891, 1557, 1570, 1748, 1761
AcoI	YGGCCR	1 cut(s)	382
AcsI	RAATTY	2 cut(s)	675, 722
AcuI	CTGAAG	5 cut(s)	227, 233, 930, 1040, 1471
AcyI	GRCGYC	2 cut(s)	78, 275
AfaI	GTAC	7 cut(s)	107, 561, 776, 922, 1133, 1538, 1556
AfiI	CCNNNNNNNGG	3 cut(s)	946, 1126, 1330
AflII	CTTAAG	1 cut(s)	401
AgsI	TTSAA	4 cut(s)	316, 611, 932, 1440
AhdI	GACNNNNNGTC	1 cut(s)	82
AjnI	CCWGG	3 cut(s)	629, 1119, 1327
AloI	GAACNNNNNNTCC	2 cut(s)	1714, 1746
Alw21I	GWGCWC	5 cut(s)	393, 419, 497, 1279, 1501
Alw26I	GTCTC	4 cut(s)	560, 953, 1009, 1232
Alw44I	GTGCAC	1 cut(s)	1497
AlwI	GGATC	6 cut(s)	597, 891, 1557, 1570, 1748, 1761
AlwNI	CAGNNNCTG	1 cut(s)	1739
Ama87I	CYCGRG	2 cut(s)	167, 563
Aor13HI	TCCGGA	1 cut(s)	330
AoxI	GGCC	3 cut(s)	382, 436, 1363
ApaLI	GTGCAC	1 cut(s)	1497
ApeKI	GCWGC	5 cut(s)	193, 863, 866, 1116, 1519
ApoI	RAATTY	2 cut(s)	675, 722
ArsI	GACNNNNNNTTYG	4 cut(s)	466, 498, 790, 822
AspLEI	GCGC	5 cut(s)	121, 129, 148, 373, 1531
AspS9I	GGNCC	4 cut(s)	70, 474, 1363, 1715
AsuC2I	CCSGG	2 cut(s)	74, 421
AsuHPI	GGTGA	3 cut(s)	536, 1150, 1421
AsuII	TTCGAA	1 cut(s)	1303
AvaI	CYCGRG	2 cut(s)	167, 563
AvaII	GGWCC	3 cut(s)	70, 474, 1715
BaeGI	GKGCMC	2 cut(s)	941, 1501
BamHI	GGATCC	2 cut(s)	1562, 1753
BanII	GRGCYC	3 cut(s)	393, 497, 1279
BbsI	GAAGAC	2 cut(s)	618, 918
Bbv12I	GWGCWC	5 cut(s)	393, 419, 497, 1279, 1501
BbvI	GCAGC	5 cut(s)	205, 853, 875, 1103, 1531
BccI	CCATC	5 cut(s)	149, 1067, 1093, 1380, 1412
BceAI	ACGGC	5 cut(s)	34, 65, 262, 369, 451
BciT130I	CCWGG	3 cut(s)	631, 1121, 1329
BclI	TGATCA	1 cut(s)	760
BcnI	CCSGG	2 cut(s)	74, 421
BcoDI	GTCTC	4 cut(s)	560, 953, 1009, 1232
BfaI	CTAG	4 cut(s)	1397, 1452, 1466, 1629
BfmI	CTRYAG	2 cut(s)	460, 1457
BfrI	CTTAAG	1 cut(s)	401
BglII	AGATCT	1 cut(s)	682
BisI	GCNGC	7 cut(s)	194, 231, 382, 864, 867, 1117, 1520
BlsI	GCNGC	7 cut(s)	195, 232, 383, 865, 868, 1118, 1521
BmcAI	AGTACT	2 cut(s)	776, 1133
Bme1390I	CCNGG	5 cut(s)	74, 421, 631, 1121, 1329
Bme18I	GGWCC	3 cut(s)	70, 474, 1715
BmeRI	GACNNNNNGTC	1 cut(s)	82
BmeT110I	CYCGRG	2 cut(s)	167, 563
BmgT120I	GGNCC	4 cut(s)	70, 474, 1363, 1715
BmiI	GGNNCC	5 cut(s)	188, 736, 1028, 1564, 1755
BmrFI	CCNGG	5 cut(s)	74, 421, 631, 1121, 1329
BmsI	GCATC	6 cut(s)	36, 1175, 1280, 1380, 1506, 1687
BpiI	GAAGAC	2 cut(s)	618, 918
BplI	GAGNNNNNCTC	4 cut(s)	479, 511, 761, 793
BpmI	CTGGAG	1 cut(s)	1148
Bpu10I	CCTNAGC	2 cut(s)	176, 1532
Bpu14I	TTCGAA	1 cut(s)	1303
BpuEI	CTTGAG	2 cut(s)	182, 977
BpuMI	CCSGG	2 cut(s)	74, 421
Bsa29I	ATCGAT	1 cut(s)	748
BsaAI	YACGTR	2 cut(s)	1147, 1360
BsaHI	GRCGYC	2 cut(s)	78, 275
BsaJI	CCNNGG	4 cut(s)	224, 278, 1328, 1749
BsaWI	WCCGGW	2 cut(s)	250, 330
BsaXI	ACNNNNNCTCC	6 cut(s)	148, 178, 255, 285, 401, 431
Bsc4I	CCNNNNNNNGG	3 cut(s)	946, 1126, 1330
Bse1I	ACTGG	5 cut(s)	34, 1131, 1366, 1506, 1757
Bse3DI	GCAATG	2 cut(s)	896, 1596
BseAI	TCCGGA	1 cut(s)	330
BseBI	CCWGG	3 cut(s)	631, 1121, 1329
BseCI	ATCGAT	1 cut(s)	748
BseDI	CCNNGG	4 cut(s)	224, 278, 1328, 1749
BseGI	GGATG	4 cut(s)	520, 1295, 1395, 1678
BseLI	CCNNNNNNNGG	3 cut(s)	946, 1126, 1330
BseMI	GCAATG	2 cut(s)	896, 1596
BseMII	CTCAG	2 cut(s)	190, 792
BseNI	ACTGG	5 cut(s)	34, 1131, 1366, 1506, 1757
BseRI	GAGGAG	2 cut(s)	302, 503
BseSI	GKGCMC	2 cut(s)	941, 1501
BseX3I	CGGCCG	1 cut(s)	382
BseXI	GCAGC	5 cut(s)	205, 853, 875, 1103, 1531
Bsh1236I	CGCG	6 cut(s)	119, 127, 129, 146, 373, 381
Bsh1285I	CGRYCG	1 cut(s)	385
BshFI	GGCC	3 cut(s)	384, 438, 1365
BshVI	ATCGAT	1 cut(s)	748
BsiEI	CGRYCG	1 cut(s)	385
BsiHKAI	GWGCWC	5 cut(s)	393, 419, 497, 1279, 1501
BsiHKCI	CYCGRG	2 cut(s)	167, 563
BsiSI	CCGG	5 cut(s)	73, 190, 251, 331, 420
BslFI	GGGAC	3 cut(s)	22, 89, 1198
BslI	CCNNNNNNNGG	3 cut(s)	946, 1126, 1330
BsmAI	GTCTC	4 cut(s)	560, 953, 1009, 1232
BsmBI	CGTCTC	1 cut(s)	1232
BsmFI	GGGAC	3 cut(s)	22, 89, 1198
BsnI	GGCC	3 cut(s)	384, 438, 1365
BsoBI	CYCGRG	2 cut(s)	167, 563
Bsp119I	TTCGAA	1 cut(s)	1303
Bsp1286I	GDGCHC	6 cut(s)	393, 419, 497, 941, 1279, 1501
Bsp13I	TCCGGA	1 cut(s)	330
BspACI	CCGC	5 cut(s)	184, 230, 373, 381, 454
BspANI	GGCC	3 cut(s)	384, 438, 1365
BspCNI	CTCAG	2 cut(s)	189, 791
BspDI	ATCGAT	1 cut(s)	748
BspEI	TCCGGA	1 cut(s)	330
BspFNI	CGCG	6 cut(s)	119, 127, 129, 146, 373, 381
BspLI	GGNNCC	5 cut(s)	188, 736, 1028, 1564, 1755
BspMAI	CTGCAG	2 cut(s)	464, 1461
BspPI	GGATC	6 cut(s)	597, 891, 1557, 1570, 1748, 1761
BspT104I	TTCGAA	1 cut(s)	1303
BspTI	CTTAAG	1 cut(s)	401
BsrBI	CCGCTC	1 cut(s)	456
BsrDI	GCAATG	2 cut(s)	896, 1596
BsrI	ACTGG	5 cut(s)	34, 1131, 1366, 1506, 1757
BssECI	CCNNGG	4 cut(s)	224, 278, 1328, 1749
BssNI	GRCGYC	2 cut(s)	78, 275
BssT1I	CCWWGG	1 cut(s)	1749
Bst2UI	CCWGG	3 cut(s)	631, 1121, 1329
Bst4CI	ACNGT	5 cut(s)	102, 559, 655, 1232, 1741
Bst6I	CTCTTC	2 cut(s)	1016, 1230
BstACI	GRCGYC	2 cut(s)	78, 275
BstAFI	CTTAAG	1 cut(s)	401
BstBAI	YACGTR	2 cut(s)	1147, 1360
BstBI	TTCGAA	1 cut(s)	1303
BstC8I	GCNNGC	5 cut(s)	131, 454, 543, 1497, 1597
BstDEI	CTNAG	4 cut(s)	176, 778, 1352, 1532
BstDSI	CCRYGG	1 cut(s)	278
BstF5I	GGATG	4 cut(s)	520, 1295, 1395, 1678
BstFNI	CGCG	6 cut(s)	119, 127, 129, 146, 373, 381
BstHHI	GCGC	5 cut(s)	121, 129, 148, 373, 1531
BstMAI	GTCTC	4 cut(s)	560, 953, 1009, 1232
BstMCI	CGRYCG	1 cut(s)	385
BstMWI	GCNNNNNNNGC	8 cut(s)	118, 181, 190, 202, 370, 397, 538, 1528
BstNI	CCWGG	3 cut(s)	631, 1121, 1329
BstSCI	CCNGG	5 cut(s)	72, 419, 629, 1119, 1327
BstSFI	CTRYAG	2 cut(s)	460, 1457
BstSLI	GKGCMC	2 cut(s)	941, 1501
BstSNI	TACGTA	1 cut(s)	1147
BstUI	CGCG	6 cut(s)	119, 127, 129, 146, 373, 381
BstV1I	GCAGC	5 cut(s)	205, 853, 875, 1103, 1531
BstV2I	GAAGAC	2 cut(s)	618, 918
BstX2I	RGATCY	5 cut(s)	589, 682, 883, 1562, 1753
BstYI	RGATCY	5 cut(s)	589, 682, 883, 1562, 1753
BstZI	CGGCCG	1 cut(s)	382
Bsu15I	ATCGAT	1 cut(s)	748
BsuRI	GGCC	3 cut(s)	384, 438, 1365
BsuTUI	ATCGAT	1 cut(s)	748
BtgI	CCRYGG	1 cut(s)	278
BtgZI	GCGATG	3 cut(s)	127, 248, 433
BtsCI	GGATG	4 cut(s)	520, 1295, 1395, 1678
BtsI	GCAGTG	2 cut(s)	788, 1518
BtsIMutI	CAGTG	7 cut(s)	555, 651, 788, 906, 1495, 1499, 1518
Cac8I	GCNNGC	5 cut(s)	131, 454, 543, 1497, 1597
CaiI	CAGNNNCTG	1 cut(s)	1739
CfoI	GCGC	5 cut(s)	121, 129, 148, 373, 1531
Cfr13I	GGNCC	4 cut(s)	70, 474, 1363, 1715
ClaI	ATCGAT	1 cut(s)	748
CseI	GACGC	3 cut(s)	86, 125, 283
Csp6I	GTAC	7 cut(s)	106, 560, 775, 921, 1132, 1537, 1555
CviAII	CATG	3 cut(s)	41, 536, 704
CviQI	GTAC	7 cut(s)	106, 560, 775, 921, 1132, 1537, 1555
DdeI	CTNAG	4 cut(s)	176, 778, 1352, 1532
DraI	TTTAAA	1 cut(s)	573
DrdI	GACNNNNNNGTC	1 cut(s)	440
DriI	GACNNNNNGTC	1 cut(s)	82
DseDI	GACNNNNNNGTC	1 cut(s)	440
EaeI	YGGCCR	1 cut(s)	382
EagI	CGGCCG	1 cut(s)	382
Eam1104I	CTCTTC	2 cut(s)	1016, 1230
Eam1105I	GACNNNNNGTC	1 cut(s)	82
EarI	CTCTTC	2 cut(s)	1016, 1230
Ecl136II	GAGCTC	3 cut(s)	391, 495, 1277
EclXI	CGGCCG	1 cut(s)	382
Eco105I	TACGTA	1 cut(s)	1147
Eco130I	CCWWGG	1 cut(s)	1749
Eco24I	GRGCYC	3 cut(s)	393, 497, 1279
Eco47I	GGWCC	3 cut(s)	70, 474, 1715
Eco52I	CGGCCG	1 cut(s)	382
Eco53kI	GAGCTC	3 cut(s)	391, 495, 1277
Eco57I	CTGAAG	5 cut(s)	227, 233, 930, 1040, 1471
Eco88I	CYCGRG	2 cut(s)	167, 563
EcoICRI	GAGCTC	3 cut(s)	391, 495, 1277
EcoO109I	RGGNCCY	1 cut(s)	474
EcoRII	CCWGG	3 cut(s)	629, 1119, 1327
EcoT14I	CCWWGG	1 cut(s)	1749
EcoT22I	ATGCAT	2 cut(s)	898, 1168
EcoT38I	GRGCYC	3 cut(s)	393, 497, 1279
ErhI	CCWWGG	1 cut(s)	1749
Esp3I	CGTCTC	1 cut(s)	1232
FaeI	CATG	3 cut(s)	44, 539, 707
FalI	AAGNNNNNCTT	2 cut(s)	1297, 1329
FaqI	GGGAC	3 cut(s)	22, 89, 1198
FatI	CATG	3 cut(s)	40, 535, 703
FauI	CCCGC	1 cut(s)	461
FbaI	TGATCA	1 cut(s)	760
FblI	GTMKAC	1 cut(s)	1143
Fnu4HI	GCNGC	7 cut(s)	194, 231, 382, 864, 867, 1117, 1520
FokI	GGATG	4 cut(s)	527, 1302, 1402, 1665
FriOI	GRGCYC	3 cut(s)	393, 497, 1279
Fsp4HI	GCNGC	7 cut(s)	194, 231, 382, 864, 867, 1117, 1520
FspBI	CTAG	4 cut(s)	1397, 1452, 1466, 1629
GlaI	GCGC	5 cut(s)	120, 128, 147, 372, 1530
GluI	GCNGC	7 cut(s)	194, 231, 382, 864, 867, 1117, 1520
GsuI	CTGGAG	1 cut(s)	1148
HaeIII	GGCC	3 cut(s)	384, 438, 1365
HapII	CCGG	5 cut(s)	73, 190, 251, 331, 420
HgaI	GACGC	3 cut(s)	86, 125, 283
HhaI	GCGC	5 cut(s)	121, 129, 148, 373, 1531
Hin1I	GRCGYC	2 cut(s)	78, 275
Hin1II	CATG	3 cut(s)	44, 539, 707
Hin6I	GCGC	5 cut(s)	119, 127, 146, 371, 1529
HinP1I	GCGC	5 cut(s)	119, 127, 146, 371, 1529
HindIII	AAGCTT	1 cut(s)	1298
HinfI	GANTC	8 cut(s)	62, 163, 299, 350, 600, 1186, 1412, 1621
HpaII	CCGG	5 cut(s)	73, 190, 251, 331, 420
HphI	GGTGA	3 cut(s)	536, 1150, 1421
Hpy166II	GTNNAC	3 cut(s)	445, 1144, 1499
Hpy188I	TCNGA	9 cut(s)	246, 355, 605, 754, 769, 1005, 1031, 1098, 1417
Hpy188III	TCNNGA	9 cut(s)	167, 178, 331, 565, 956, 1172, 1577, 1646, 1733
Hpy8I	GTNNAC	3 cut(s)	445, 1144, 1499
Hpy99I	CGWCG	2 cut(s)	102, 163
HpyAV	CCTTC	5 cut(s)	61, 208, 310, 748, 1299
HpyCH4III	ACNGT	5 cut(s)	102, 559, 655, 1232, 1741
HpyCH4IV	ACGT	3 cut(s)	15, 1146, 1359
HpyF10VI	GCNNNNNNNGC	8 cut(s)	118, 181, 190, 202, 370, 397, 538, 1528
HpyF3I	CTNAG	4 cut(s)	176, 778, 1352, 1532
HpySE526I	ACGT	3 cut(s)	15, 1146, 1359
Hsp92I	GRCGYC	2 cut(s)	78, 275
Hsp92II	CATG	3 cut(s)	44, 539, 707
HspAI	GCGC	5 cut(s)	119, 127, 146, 371, 1529
Kpn2I	TCCGGA	1 cut(s)	330
Ksp22I	TGATCA	1 cut(s)	760
LmnI	GCTCC	5 cut(s)	361, 396, 422, 461, 1032
Lsp1109I	GCAGC	5 cut(s)	205, 853, 875, 1103, 1531
LweI	GCATC	6 cut(s)	36, 1175, 1280, 1380, 1506, 1687
MaeI	CTAG	4 cut(s)	1397, 1452, 1466, 1629
MaeII	ACGT	3 cut(s)	15, 1146, 1359
MaeIII	GTNAC	4 cut(s)	136, 271, 375, 1355
MbiI	CCGCTC	1 cut(s)	456
MflI	RGATCY	5 cut(s)	589, 682, 883, 1562, 1753
MhlI	GDGCHC	6 cut(s)	393, 419, 497, 941, 1279, 1501
MluCI	AATT	7 cut(s)	501, 634, 660, 675, 722, 927, 1032
MlyI	GAGTC	4 cut(s)	56, 157, 308, 1180
MmeI	TCCRAC	2 cut(s)	378, 861
Mph1103I	ATGCAT	2 cut(s)	898, 1168
MroI	TCCGGA	1 cut(s)	330
MseI	TTAA	7 cut(s)	240, 306, 402, 572, 647, 926, 1768
MspA1I	CMGCKG	2 cut(s)	866, 1522
MspCI	CTTAAG	1 cut(s)	401
MspI	CCGG	5 cut(s)	73, 190, 251, 331, 420
MspR9I	CCNGG	5 cut(s)	74, 421, 631, 1121, 1329
MvaI	CCWGG	3 cut(s)	631, 1121, 1329
MvnI	CGCG	6 cut(s)	119, 127, 129, 146, 373, 381
MwoI	GCNNNNNNNGC	8 cut(s)	118, 181, 190, 202, 370, 397, 538, 1528
NciI	CCSGG	2 cut(s)	74, 421
NlaIII	CATG	3 cut(s)	44, 539, 707
NlaIV	GGNNCC	5 cut(s)	188, 736, 1028, 1564, 1755
NmeAIII	GCCGAG	2 cut(s)	249, 322
NmuCI	GTSAC	3 cut(s)	136, 271, 375
NsiI	ATGCAT	2 cut(s)	898, 1168
NspV	TTCGAA	1 cut(s)	1303
PaeR7I	CTCGAG	1 cut(s)	563
PfeI	GAWTC	4 cut(s)	350, 600, 1412, 1621
PkrI	GCNGC	7 cut(s)	195, 232, 383, 865, 868, 1118, 1521
PleI	GAGTC	4 cut(s)	56, 157, 307, 1180
PpsI	GAGTC	4 cut(s)	56, 157, 307, 1180
Ppu21I	YACGTR	2 cut(s)	1147, 1360
PpuMI	RGGWCCY	1 cut(s)	474
Psp124BI	GAGCTC	3 cut(s)	393, 497, 1279
Psp5II	RGGWCCY	1 cut(s)	474
Psp6I	CCWGG	3 cut(s)	629, 1119, 1327
PspGI	CCWGG	3 cut(s)	629, 1119, 1327
PspN4I	GGNNCC	5 cut(s)	188, 736, 1028, 1564, 1755
PspPI	GGNCC	4 cut(s)	70, 474, 1363, 1715
PspPPI	RGGWCCY	1 cut(s)	474
PstI	CTGCAG	2 cut(s)	464, 1461
PstNI	CAGNNNCTG	1 cut(s)	1739
PsuI	RGATCY	5 cut(s)	589, 682, 883, 1562, 1753
PvuII	CAGCTG	2 cut(s)	866, 1522
RsaI	GTAC	7 cut(s)	107, 561, 776, 922, 1133, 1538, 1556
RsaNI	GTAC	7 cut(s)	106, 560, 775, 921, 1132, 1537, 1555
SacI	GAGCTC	3 cut(s)	393, 497, 1279
SaqAI	TTAA	7 cut(s)	240, 306, 402, 572, 647, 926, 1768
SatI	GCNGC	7 cut(s)	194, 231, 382, 864, 867, 1117, 1520
Sau96I	GGNCC	4 cut(s)	70, 474, 1363, 1715
SbfI	CCTGCAGG	1 cut(s)	464
ScaI	AGTACT	2 cut(s)	776, 1133
SchI	GAGTC	4 cut(s)	56, 157, 308, 1180
ScrFI	CCNGG	5 cut(s)	74, 421, 631, 1121, 1329
SdaI	CCTGCAGG	1 cut(s)	464
SduI	GDGCHC	6 cut(s)	393, 419, 497, 941, 1279, 1501
SfaNI	GCATC	6 cut(s)	36, 1175, 1280, 1380, 1506, 1687
SfcI	CTRYAG	2 cut(s)	460, 1457
Sfr274I	CTCGAG	1 cut(s)	563
SfuI	TTCGAA	1 cut(s)	1303
SinI	GGWCC	3 cut(s)	70, 474, 1715
SlaI	CTCGAG	1 cut(s)	563
SmlI	CTYRAG	4 cut(s)	197, 401, 563, 956
SmoI	CTYRAG	4 cut(s)	197, 401, 563, 956
SnaBI	TACGTA	1 cut(s)	1147
Sse8387I	CCTGCAGG	1 cut(s)	464
Sse9I	AATT	7 cut(s)	501, 634, 660, 675, 722, 927, 1032
SsiI	CCGC	5 cut(s)	184, 230, 373, 381, 454
SspI	AATATT	1 cut(s)	1612
SspMI	CTAG	4 cut(s)	1397, 1452, 1466, 1629
SstI	GAGCTC	3 cut(s)	393, 497, 1279
StyD4I	CCNGG	5 cut(s)	72, 419, 629, 1119, 1327
StyI	CCWWGG	1 cut(s)	1749
TaaI	ACNGT	5 cut(s)	102, 559, 655, 1232, 1741
TaiI	ACGT	3 cut(s)	18, 1149, 1362
TasI	AATT	7 cut(s)	501, 634, 660, 675, 722, 927, 1032
TatI	WGTACW	4 cut(s)	559, 774, 920, 1131
TauI	GCSGC	2 cut(s)	233, 384
TfiI	GAWTC	4 cut(s)	350, 600, 1412, 1621
Tru1I	TTAA	7 cut(s)	240, 306, 402, 572, 647, 926, 1768
Tru9I	TTAA	7 cut(s)	240, 306, 402, 572, 647, 926, 1768
TscAI	CASTG	7 cut(s)	562, 658, 795, 913, 1495, 1506, 1518
TseFI	GTSAC	3 cut(s)	136, 271, 375
TseI	GCWGC	5 cut(s)	193, 863, 866, 1116, 1519
Tsp45I	GTSAC	3 cut(s)	136, 271, 375
TspDTI	ATGAA	2 cut(s)	38, 852
TspGWI	ACGGA	3 cut(s)	602, 689, 1313
TspRI	CASTG	7 cut(s)	562, 658, 795, 913, 1495, 1506, 1518
Vha464I	CTTAAG	1 cut(s)	401
VneI	GTGCAC	1 cut(s)	1497
VpaK11BI	GGWCC	3 cut(s)	70, 474, 1715
XapI	RAATTY	2 cut(s)	675, 722
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1168
XhoI	CTCGAG	1 cut(s)	563
XmiI	GTMKAC	1 cut(s)	1143
XspI	CTAG	4 cut(s)	1397, 1452, 1466, 1629
ZrmI	AGTACT	2 cut(s)	776, 1133
Zsp2I	ATGCAT	2 cut(s)	898, 1168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.