Rorug02G0018100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012558
Physical Location & Seq
Forward (+)
1489867 .. 1491891
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0018100.1

Sequence Viewer

Length: 870 bp
ATGAACAAATACGAACGTGTCCGAACGCTTTCCAAGACTGGGAGGTACGGCGAAGTCTACTCCGTGAGGAACAAGCAGACGAATGAGTATTTCATCGCCCAAGAATTCAATCGGAGAGGCCTTGAAGTTGCCCGGCGCGAATGCGGGTTCCTGGACGAGCTATCCGGGTCGGGTCATGTTATCAAGAAAGTGGAGGAAATCCAGAACTCCGGTGAGCGACTCGCCGTCACGGTGGTCTACGAAGCTCTCGCCGCGACAAGTCTGAGGCGGTTCTTGGACGCCAACCCCGAACAGCCTCTGCCGCTCCGGGCCGTCAAATCGATCACATACCAAATCTTGAAGGGCTTGGCCGACGCTCACAGCCTGGGCATCTTTCACCGTGACATCCGCCCCAGTAAGATCGCCGTCGCGGTCGCCGACGATGGGTCGTTGATAATCAAGATAGTGTGGATTTCAGGGACGCCAGAAGGGTGGGTCCACCGGAACCCGTCACTCCGACTAGAGACTTCGAAAAGGGGCAGGGTTTTGTTCCCTGGAATGAACCTTATCGATTCAAACAGGACACTAATTCAACCCAGGGAGCAGGATACCGAGACACAGTATCTAGGTCAGCTCTACAAGATTTTCAGTATACGCGGTACTCCTCGCCTTCACCTTCTACCAGCAGATGTAGCTGGCTTTCTGGACTGGGAGACTGTCCCTCGGTTTCGCAGAGCCAACCTCCTCCGACGTTTCCCTATGCTTGGTGTTTCTGGCAATCGGCTCCTGGCGCGTTTGTTGAGCTACTCTCCAGTAGAAAGGCTGTCAGCTGCTGCCGCGATTGGGCACGACTTTTTCGAGGAACTGCGCCCTCAATCAGATGCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

32.79

Weight (kDa)

9.73

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 13 - 137 1.6e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 304
AccI GTMKAC 3 cut(s) 57, 237, 631
AccII CGCG 6 cut(s) 138, 254, 410, 636, 772, 818
AciI CCGC 8 cut(s) 144, 252, 268, 302, 388, 410, 636, 816
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 279, 461
AfaI GTAC 2 cut(s) 47, 640
AfiI CCNNNNNNNGG 4 cut(s) 39, 423, 743, 822
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 5 cut(s) 109, 125, 340, 555, 572
AhdI GACNNNNNGTC 2 cut(s) 224, 424
AjnI CCWGG 5 cut(s) 150, 363, 532, 575, 765
AluBI AGCT 6 cut(s) 160, 245, 613, 674, 783, 809
AluI AGCT 6 cut(s) 160, 245, 613, 674, 783, 809
Alw26I GTCTC 3 cut(s) 497, 587, 686
AlwNI CAGNNNCTG 2 cut(s) 298, 812
AoxI GGCC 3 cut(s) 118, 309, 348
ApeKI GCWGC 2 cut(s) 809, 812
ApoI RAATTY 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 28
AspLEI GCGC 3 cut(s) 138, 772, 849
AspS9I GGNCC 2 cut(s) 309, 475
AsuC2I CCSGG 3 cut(s) 133, 166, 308
AsuHPI GGTGA 3 cut(s) 224, 368, 644
AsuII TTCGAA 1 cut(s) 509
AvaII GGWCC 1 cut(s) 475
BaeGI GKGCMC 1 cut(s) 828
BbvI GCAGC 2 cut(s) 796, 799
BccI CCATC 1 cut(s) 416
BceAI ACGGC 4 cut(s) 64, 209, 296, 389
BciT130I CCWGG 5 cut(s) 152, 365, 534, 577, 767
BciVI GTATCC 1 cut(s) 580
BcnI CCSGG 3 cut(s) 133, 166, 308
BcoDI GTCTC 3 cut(s) 497, 587, 686
BfaI CTAG 2 cut(s) 500, 605
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 5 cut(s) 252, 302, 810, 813, 816
BlsI GCNGC 5 cut(s) 253, 303, 811, 814, 817
Bme1390I CCNGG 8 cut(s) 133, 152, 166, 308, 365, 534, 577, 767
Bme18I GGWCC 1 cut(s) 475
BmeRI GACNNNNNGTC 2 cut(s) 224, 424
BmgT120I GGNCC 2 cut(s) 309, 475
BmiI GGNNCC 4 cut(s) 149, 476, 485, 764
BmrFI CCNGG 8 cut(s) 133, 152, 166, 308, 365, 534, 577, 767
BmrI ACTGGG 3 cut(s) 48, 387, 697
BmsI GCATC 2 cut(s) 378, 850
BmuI ACTGGG 3 cut(s) 48, 387, 697
BplI GAGNNNNNCTC 4 cut(s) 705, 737, 772, 804
BpmI CTGGAG 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 509
BpuMI CCSGG 3 cut(s) 133, 166, 308
Bsa29I ATCGAT 2 cut(s) 320, 549
BsaHI GRCGYC 2 cut(s) 279, 461
BsaJI CCNNGG 5 cut(s) 364, 532, 575, 576, 701
BsaWI WCCGGW 2 cut(s) 209, 480
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 423, 743, 822
Bse1I ACTGG 4 cut(s) 43, 393, 692, 791
BseBI CCWGG 5 cut(s) 152, 365, 534, 577, 767
BseCI ATCGAT 2 cut(s) 320, 549
BseDI CCNNGG 5 cut(s) 364, 532, 575, 576, 701
BseGI GGATG 1 cut(s) 384
BseLI CCNNNNNNNGG 4 cut(s) 39, 423, 743, 822
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 4 cut(s) 43, 393, 692, 791
BseRI GAGGAG 2 cut(s) 633, 713
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 2 cut(s) 796, 799
Bsh1236I CGCG 6 cut(s) 138, 254, 410, 636, 772, 818
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 3 cut(s) 120, 311, 350
BshVI ATCGAT 2 cut(s) 320, 549
BsiEI CGRYCG 1 cut(s) 414
BsiSI CCGG 5 cut(s) 133, 165, 210, 307, 481
BslFI GGGAC 2 cut(s) 472, 683
BslI CCNNNNNNNGG 4 cut(s) 39, 423, 743, 822
BsmAI GTCTC 3 cut(s) 497, 587, 686
BsmFI GGGAC 2 cut(s) 472, 683
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 3 cut(s) 120, 311, 350
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 2 cut(s) 321, 399
BspACI CCGC 8 cut(s) 144, 252, 268, 302, 388, 410, 636, 816
BspANI GGCC 3 cut(s) 120, 311, 350
BspCNI CTCAG 1 cut(s) 255
BspDI ATCGAT 2 cut(s) 320, 549
BspFNI CGCG 6 cut(s) 138, 254, 410, 636, 772, 818
BspLI GGNNCC 4 cut(s) 149, 476, 485, 764
BspT104I TTCGAA 1 cut(s) 509
BsrBI CCGCTC 1 cut(s) 304
BsrI ACTGG 4 cut(s) 43, 393, 692, 791
BssECI CCNNGG 5 cut(s) 364, 532, 575, 576, 701
BssMI GATC 2 cut(s) 321, 399
BssNAI GTATAC 1 cut(s) 632
BssNI GRCGYC 2 cut(s) 279, 461
Bst1107I GTATAC 1 cut(s) 632
Bst2UI CCWGG 5 cut(s) 152, 365, 534, 577, 767
Bst4CI ACNGT 4 cut(s) 232, 380, 600, 697
BstACI GRCGYC 2 cut(s) 279, 461
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 676
BstDEI CTNAG 1 cut(s) 263
BstF5I GGATG 1 cut(s) 384
BstFNI CGCG 6 cut(s) 138, 254, 410, 636, 772, 818
BstHHI GCGC 3 cut(s) 138, 772, 849
BstKTI GATC 2 cut(s) 324, 402
BstMAI GTCTC 3 cut(s) 497, 587, 686
BstMBI GATC 2 cut(s) 321, 399
BstMCI CGRYCG 1 cut(s) 414
BstMWI GCNNNNNNNGC 5 cut(s) 251, 301, 671, 769, 815
BstNI CCWGG 5 cut(s) 152, 365, 534, 577, 767
BstSCI CCNGG 8 cut(s) 131, 150, 164, 306, 363, 532, 575, 765
BstSLI GKGCMC 1 cut(s) 828
BstUI CGCG 6 cut(s) 138, 254, 410, 636, 772, 818
BstV1I GCAGC 2 cut(s) 796, 799
BstXI CCANNNNNNTGG 1 cut(s) 471
BstZ17I GTATAC 1 cut(s) 632
Bsu15I ATCGAT 2 cut(s) 320, 549
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 120, 311, 350
BsuTUI ATCGAT 2 cut(s) 320, 549
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 1 cut(s) 384
Cac8I GCNNGC 1 cut(s) 676
CaiI CAGNNNCTG 2 cut(s) 298, 812
CfoI GCGC 3 cut(s) 138, 772, 849
Cfr13I GGNCC 2 cut(s) 309, 475
ClaI ATCGAT 2 cut(s) 320, 549
CseI GACGC 3 cut(s) 287, 362, 469
Csp6I GTAC 2 cut(s) 46, 639
CviAII CATG 1 cut(s) 176
CviQI GTAC 2 cut(s) 46, 639
DdeI CTNAG 1 cut(s) 263
DpnI GATC 2 cut(s) 323, 401
DpnII GATC 2 cut(s) 321, 399
DriI GACNNNNNGTC 2 cut(s) 224, 424
EaeI YGGCCR 1 cut(s) 348
Eam1105I GACNNNNNGTC 2 cut(s) 224, 424
EciI GGCGGA 1 cut(s) 377
Eco147I AGGCCT 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 475
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 5 cut(s) 150, 363, 532, 575, 765
FaeI CATG 1 cut(s) 179
FaiI YATR 4 cut(s) 177, 328, 632, 740
FaqI GGGAC 2 cut(s) 472, 683
FatI CATG 1 cut(s) 175
FauI CCCGC 1 cut(s) 137
FblI GTMKAC 3 cut(s) 57, 237, 631
Fnu4HI GCNGC 5 cut(s) 252, 302, 810, 813, 816
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 5 cut(s) 252, 302, 810, 813, 816
FspBI CTAG 2 cut(s) 500, 605
GlaI GCGC 3 cut(s) 137, 771, 848
GluI GCNGC 5 cut(s) 252, 302, 810, 813, 816
GsuI CTGGAG 1 cut(s) 774
HaeIII GGCC 3 cut(s) 120, 311, 350
HapII CCGG 5 cut(s) 133, 165, 210, 307, 481
HgaI GACGC 3 cut(s) 287, 362, 469
HhaI GCGC 3 cut(s) 138, 772, 849
Hin1I GRCGYC 2 cut(s) 279, 461
Hin1II CATG 1 cut(s) 179
Hin6I GCGC 3 cut(s) 136, 770, 847
HinP1I GCGC 3 cut(s) 136, 770, 847
HinfI GANTC 2 cut(s) 219, 551
HpaII CCGG 5 cut(s) 133, 165, 210, 307, 481
HphI GGTGA 3 cut(s) 224, 368, 644
Hpy166II GTNNAC 4 cut(s) 58, 238, 478, 632
Hpy188I TCNGA 6 cut(s) 23, 114, 264, 497, 728, 859
Hpy188III TCNNGA 5 cut(s) 184, 202, 337, 439, 683
Hpy8I GTNNAC 4 cut(s) 58, 238, 478, 632
Hpy99I CGWCG 4 cut(s) 356, 410, 422, 732
HpyAV CCTTC 4 cut(s) 334, 461, 659, 665
HpyCH4III ACNGT 4 cut(s) 232, 380, 600, 697
HpyCH4IV ACGT 2 cut(s) 16, 730
HpyCH4V TGCA 1 cut(s) 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 301, 671, 769, 815
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 2 cut(s) 16, 730
Hsp92I GRCGYC 2 cut(s) 279, 461
Hsp92II CATG 1 cut(s) 179
HspAI GCGC 3 cut(s) 136, 770, 847
Kzo9I GATC 2 cut(s) 321, 399
LmnI GCTCC 3 cut(s) 309, 580, 768
Lsp1109I GCAGC 2 cut(s) 796, 799
LweI GCATC 2 cut(s) 378, 850
MaeI CTAG 2 cut(s) 500, 605
MaeII ACGT 2 cut(s) 16, 730
MaeIII GTNAC 3 cut(s) 226, 380, 489
MalI GATC 2 cut(s) 323, 401
MbiI CCGCTC 1 cut(s) 304
MboI GATC 2 cut(s) 321, 399
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 2 cut(s) 104, 567
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 2 cut(s) 520, 751
MroXI GAANNNNTTC 1 cut(s) 28
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 5 cut(s) 133, 165, 210, 307, 481
MspR9I CCNGG 8 cut(s) 133, 152, 166, 308, 365, 534, 577, 767
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 5 cut(s) 152, 365, 534, 577, 767
MvnI CGCG 6 cut(s) 138, 254, 410, 636, 772, 818
MwoI GCNNNNNNNGC 5 cut(s) 251, 301, 671, 769, 815
NciI CCSGG 3 cut(s) 133, 166, 308
NdeII GATC 2 cut(s) 321, 399
NlaIII CATG 1 cut(s) 179
NlaIV GGNNCC 4 cut(s) 149, 476, 485, 764
NmuCI GTSAC 3 cut(s) 226, 380, 489
NspV TTCGAA 1 cut(s) 509
PasI CCCWGGG 1 cut(s) 576
PceI AGGCCT 1 cut(s) 120
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 414, 834
PctI GAATGC 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 28
PfeI GAWTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 5 cut(s) 253, 303, 811, 814, 817
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
Psp6I CCWGG 5 cut(s) 150, 363, 532, 575, 765
PspGI CCWGG 5 cut(s) 150, 363, 532, 575, 765
PspN4I GGNNCC 4 cut(s) 149, 476, 485, 764
PspPI GGNCC 2 cut(s) 309, 475
PstNI CAGNNNCTG 2 cut(s) 298, 812
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 2 cut(s) 47, 640
RsaNI GTAC 2 cut(s) 46, 639
SatI GCNGC 5 cut(s) 252, 302, 810, 813, 816
Sau3AI GATC 2 cut(s) 321, 399
Sau96I GGNCC 2 cut(s) 309, 475
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 8 cut(s) 133, 152, 166, 308, 365, 534, 577, 767
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 2 cut(s) 378, 850
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 1 cut(s) 475
Sse9I AATT 2 cut(s) 104, 567
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 8 cut(s) 144, 252, 268, 302, 388, 410, 636, 816
SspMI CTAG 2 cut(s) 500, 605
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 8 cut(s) 131, 150, 164, 306, 363, 532, 575, 765
TaaI ACNGT 4 cut(s) 232, 380, 600, 697
TaiI ACGT 2 cut(s) 19, 733
TaqI TCGA 4 cut(s) 320, 509, 549, 837
TasI AATT 2 cut(s) 104, 567
TauI GCSGC 3 cut(s) 254, 304, 818
TfiI GAWTC 1 cut(s) 551
TseFI GTSAC 3 cut(s) 226, 380, 489
TseI GCWGC 2 cut(s) 809, 812
Tsp45I GTSAC 3 cut(s) 226, 380, 489
TspDTI ATGAA 3 cut(s) 17, 82, 554
TspGWI ACGGA 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 475
XapI RAATTY 1 cut(s) 104
XmiI GTMKAC 3 cut(s) 57, 237, 631
XmnI GAANNNNTTC 1 cut(s) 28
XspI CTAG 2 cut(s) 500, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.