Rorug02G0066700

Ubiquitin domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007479
Physical Location & Seq
Forward (+)
5199415 .. 5202583
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0066700.1

Sequence Viewer

Length: 840 bp
ATGAGGATCGAGCAATTGGATGAGCCGGAGTTGGATCGGAGCGAAGGGAGGCAGATTGTGAGGGTTGACGAAGCGAAGAGAGCCCTGGTCGGAGCCGGGGCTCGGATCCTGTTTTACCCGACCCTTTTGTATAATGTGCTTCGGAATAAGATGGAAGCTGAGTTCAGATGGTGGGATGAAGTTGACCAGTTTGTGCTCCTTGGCGCGGTTCCATTCCCCAAAGATGTTCCCCAGCTGAAGCGACTTGGTGTTGGCGGCGTTATCACGCTCAACGAATCTTATGAAACTTTGGTTCCTTCCTCCCTGTATCATGCCCATGGAATTGAGCATGTAGTAATCCCCACACGTGATTATCTCTTTGCACCTTCATTTGAAGATATCAGCAGGGCTGTGGAGTTCATTCACAAGAACGCATCATGTGGTAGAACTACTTATGTTCACTGCAAAGCAGGTCGGGGAAGGAGCACTACTATTGTGCTTTGCTATTTGGTGGAATACAAGAATATGACCCCATCTGCTGCCCTGGAGTATGTGCGATCCAGAAGGCCTCGTGTGCTGCTTGCCCCCTCACAGTGGAAGGCTGTTCAAGAATACAGCAGATGTTGTAGAGCTACCACTGCATGCTCTCCCTCAGGAGATGCAGTTCTTATTACAAAAGATGATCTCGAAGGTTATCATGGCAGTTATGACAATACTGCTCGTAAGCAGTTGGCAATGGTACCTAAGATTGTGAAATCCAGCCCCATGATAGCGCGGTTGTCCTGCCTTTTTGCGTCCTTGAAAGTTTCTGGTGGACCAGTCACAAGGCGGCTGCCAGTTCCAGAGCCCCGCGCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
UBA ubiquitin

Protein Analysis

279

Amino Acids

31.32

Weight (kDa)

9.07

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-SAK PF22784 74 - 174 2.7e-13 Swiss Army Knife protein, DSP-PTPase phosphatase domain
DSPc PF00782 77 - 200 1.2e-20 Dual specificity phosphatase, catalytic domain
Tc-R-P PF22785 77 - 165 9.3e-12 Polymorphic toxin system, DSP-PTPase phosphatase
PTP-NADK PF22741 99 - 165 1.9e-06 DSP-PTPase phosphatase fused to NAD+ Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 440
Acc65I GGTACC 1 cut(s) 718
AccB1I GGYRCC 1 cut(s) 718
AccII CGCG 3 cut(s) 206, 754, 831
AciI CCGC 5 cut(s) 206, 255, 754, 808, 829
AclWI GGATC 5 cut(s) 14, 42, 100, 113, 531
AcuI CTGAAG 1 cut(s) 257
AcvI CACGTG 1 cut(s) 347
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 3 cut(s) 102, 205, 573
AflIII ACRYGT 1 cut(s) 344
AgsI TTSAA 3 cut(s) 374, 587, 781
AjnI CCWGG 2 cut(s) 84, 522
AloI GAACNNNNNNTCC 4 cut(s) 146, 178, 627, 659
AluBI AGCT 3 cut(s) 158, 235, 611
AluI AGCT 3 cut(s) 158, 235, 611
Alw21I GWGCWC 2 cut(s) 198, 467
AlwI GGATC 5 cut(s) 14, 42, 100, 113, 531
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 3 cut(s) 518, 556, 811
Asp718I GGTACC 1 cut(s) 718
AspLEI GCGC 3 cut(s) 206, 754, 833
AspS9I GGNCC 1 cut(s) 794
AsuC2I CCSGG 1 cut(s) 97
AvaII GGWCC 1 cut(s) 794
AxyI CCTNAGG 1 cut(s) 631
BamHI GGATCC 1 cut(s) 105
BanI GGYRCC 1 cut(s) 718
BanII GRGCYC 3 cut(s) 85, 103, 828
BarI GAAGNNNNNNTAC 2 cut(s) 451, 483
BauI CACGAG 1 cut(s) 549
BbrPI CACGTG 1 cut(s) 347
Bbv12I GWGCWC 2 cut(s) 198, 467
BbvI GCAGC 3 cut(s) 505, 543, 798
BccI CCATC 3 cut(s) 145, 162, 520
BciT130I CCWGG 2 cut(s) 86, 524
BcnI CCSGG 1 cut(s) 97
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 5 cut(s) 256, 519, 557, 809, 812
BlsI GCNGC 5 cut(s) 257, 520, 558, 810, 813
Bme1390I CCNGG 3 cut(s) 86, 97, 524
Bme18I GGWCC 1 cut(s) 794
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 5 cut(s) 94, 107, 210, 294, 720
BmrFI CCNGG 3 cut(s) 86, 97, 524
BmsI GCATC 2 cut(s) 422, 628
BpmI CTGGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 97
BsaAI YACGTR 1 cut(s) 347
BsaJI CCNNGG 5 cut(s) 84, 96, 199, 316, 522
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 205, 573
Bse1I ACTGG 3 cut(s) 187, 797, 815
Bse21I CCTNAGG 1 cut(s) 631
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 2 cut(s) 86, 524
BseDI CCNNGG 5 cut(s) 84, 96, 199, 316, 522
BseGI GGATG 2 cut(s) 25, 181
BseLI CCNNNNNNNGG 3 cut(s) 102, 205, 573
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 150, 645
BseNI ACTGG 3 cut(s) 187, 797, 815
BseXI GCAGC 3 cut(s) 505, 543, 798
BseYI CCCAGC 1 cut(s) 231
Bsh1236I CGCG 3 cut(s) 206, 754, 831
BshFI GGCC 1 cut(s) 547
BshNI GGYRCC 1 cut(s) 718
BsiHKAI GWGCWC 2 cut(s) 198, 467
BsiSI CCGG 2 cut(s) 26, 96
BslI CCNNNNNNNGG 3 cut(s) 102, 205, 573
BsnI GGCC 1 cut(s) 547
Bsp1286I GDGCHC 5 cut(s) 85, 103, 198, 467, 828
Bsp143I GATC 5 cut(s) 6, 34, 105, 536, 661
Bsp19I CCATGG 1 cut(s) 316
BspACI CCGC 5 cut(s) 206, 255, 754, 808, 829
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 2 cut(s) 151, 644
BspFNI CGCG 3 cut(s) 206, 754, 831
BspLI GGNNCC 5 cut(s) 94, 107, 210, 294, 720
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 5 cut(s) 14, 42, 100, 113, 531
BspT107I GGYRCC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 3 cut(s) 187, 797, 815
BssECI CCNNGG 5 cut(s) 84, 96, 199, 316, 522
BssMI GATC 5 cut(s) 6, 34, 105, 536, 661
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 199, 316
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 86, 524
Bst4CI ACNGT 1 cut(s) 573
Bst6I CTCTTC 1 cut(s) 71
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 3 cut(s) 561, 622, 835
BstDEI CTNAG 3 cut(s) 159, 631, 723
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 2 cut(s) 25, 181
BstFNI CGCG 3 cut(s) 206, 754, 831
BstHHI GCGC 3 cut(s) 206, 754, 833
BstKTI GATC 5 cut(s) 9, 37, 108, 539, 664
BstMBI GATC 5 cut(s) 6, 34, 105, 536, 661
BstMWI GCNNNNNNNGC 3 cut(s) 80, 553, 617
BstNI CCWGG 2 cut(s) 86, 524
BstNSI RCATGY 2 cut(s) 332, 624
BstSCI CCNGG 3 cut(s) 84, 95, 522
BstUI CGCG 3 cut(s) 206, 754, 831
BstV1I GCAGC 3 cut(s) 505, 543, 798
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
Bsu36I CCTNAGG 1 cut(s) 631
BsuRI GGCC 1 cut(s) 547
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 2 cut(s) 25, 181
BtsI GCAGTG 2 cut(s) 439, 615
BtsIMutI CAGTG 3 cut(s) 439, 578, 615
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 3 cut(s) 561, 622, 835
CfoI GCGC 3 cut(s) 206, 754, 833
Cfr13I GGNCC 1 cut(s) 794
CseI GACGC 1 cut(s) 762
Csp6I GTAC 1 cut(s) 719
CviAII CATG 7 cut(s) 311, 317, 329, 417, 621, 677, 745
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 3 cut(s) 159, 631, 723
DpnI GATC 5 cut(s) 8, 36, 107, 538, 663
DpnII GATC 5 cut(s) 6, 34, 105, 536, 661
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 199, 316
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 3 cut(s) 85, 103, 828
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 257
Eco72I CACGTG 1 cut(s) 347
Eco81I CCTNAGG 1 cut(s) 631
EcoRII CCWGG 2 cut(s) 84, 522
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 199, 316
EcoT38I GRGCYC 3 cut(s) 85, 103, 828
ErhI CCWWGG 2 cut(s) 199, 316
FaeI CATG 7 cut(s) 314, 320, 332, 420, 624, 680, 748
FatI CATG 7 cut(s) 310, 316, 328, 416, 620, 676, 744
FauI CCCGC 1 cut(s) 836
Fnu4HI GCNGC 5 cut(s) 256, 519, 557, 809, 812
FokI GGATG 2 cut(s) 32, 188
FriOI GRGCYC 3 cut(s) 85, 103, 828
Fsp4HI GCNGC 5 cut(s) 256, 519, 557, 809, 812
GlaI GCGC 3 cut(s) 205, 753, 832
GluI GCNGC 5 cut(s) 256, 519, 557, 809, 812
GsaI CCCAGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 1 cut(s) 547
HapII CCGG 2 cut(s) 26, 96
HgaI GACGC 1 cut(s) 762
HhaI GCGC 3 cut(s) 206, 754, 833
Hin1II CATG 7 cut(s) 314, 320, 332, 420, 624, 680, 748
Hin6I GCGC 3 cut(s) 204, 752, 831
HinP1I GCGC 3 cut(s) 204, 752, 831
HincII GTYRAC 2 cut(s) 67, 184
HindII GTYRAC 2 cut(s) 67, 184
HinfI GANTC 1 cut(s) 275
HpaII CCGG 2 cut(s) 26, 96
Hpy166II GTNNAC 4 cut(s) 67, 184, 439, 794
Hpy188I TCNGA 5 cut(s) 39, 92, 105, 144, 167
Hpy188III TCNNGA 5 cut(s) 540, 587, 633, 665, 821
Hpy8I GTNNAC 4 cut(s) 67, 184, 439, 794
HpyAV CCTTC 7 cut(s) 38, 306, 375, 453, 537, 571, 662
HpyCH4III ACNGT 1 cut(s) 573
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 4 cut(s) 362, 444, 620, 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 553, 617
HpyF3I CTNAG 3 cut(s) 159, 631, 723
HpySE526I ACGT 1 cut(s) 346
Hsp92II CATG 7 cut(s) 314, 320, 332, 420, 624, 680, 748
HspAI GCGC 3 cut(s) 204, 752, 831
KpnI GGTACC 1 cut(s) 722
Kzo9I GATC 5 cut(s) 6, 34, 105, 536, 661
LmnI GCTCC 4 cut(s) 39, 92, 201, 462
Lsp1109I GCAGC 3 cut(s) 505, 543, 798
LweI GCATC 2 cut(s) 422, 628
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 799
MalI GATC 5 cut(s) 8, 36, 107, 538, 663
MboI GATC 5 cut(s) 6, 34, 105, 536, 661
MboII GAAGA 2 cut(s) 88, 386
MfeI CAATTG 1 cut(s) 14
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 5 cut(s) 85, 103, 198, 467, 828
MluCI AATT 2 cut(s) 14, 321
MmeI TCCRAC 2 cut(s) 12, 70
MnlI CCTC 6 cut(s) 42, 54, 310, 558, 577, 640
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 2 cut(s) 26, 96
MspR9I CCNGG 3 cut(s) 86, 97, 524
MunI CAATTG 1 cut(s) 14
MvaI CCWGG 2 cut(s) 86, 524
MvnI CGCG 3 cut(s) 206, 754, 831
MwoI GCNNNNNNNGC 3 cut(s) 80, 553, 617
NciI CCSGG 1 cut(s) 97
NcoI CCATGG 1 cut(s) 316
NdeII GATC 5 cut(s) 6, 34, 105, 536, 661
NlaIII CATG 7 cut(s) 314, 320, 332, 420, 624, 680, 748
NlaIV GGNNCC 5 cut(s) 94, 107, 210, 294, 720
NmuCI GTSAC 1 cut(s) 799
NspI RCATGY 2 cut(s) 332, 624
PaeI GCATGC 1 cut(s) 624
PceI AGGCCT 1 cut(s) 547
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 5 cut(s) 257, 520, 558, 810, 813
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 347
Psp6I CCWGG 2 cut(s) 84, 522
PspCI CACGTG 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 2 cut(s) 84, 522
PspN4I GGNNCC 5 cut(s) 94, 107, 210, 294, 720
PspPI GGNCC 1 cut(s) 794
PsuI RGATCY 1 cut(s) 105
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
RseI CAYNNNNRTG 1 cut(s) 315
SatI GCNGC 5 cut(s) 256, 519, 557, 809, 812
Sau3AI GATC 5 cut(s) 6, 34, 105, 536, 661
Sau96I GGNCC 1 cut(s) 794
ScrFI CCNGG 3 cut(s) 86, 97, 524
SduI GDGCHC 5 cut(s) 85, 103, 198, 467, 828
SetI ASST 8 cut(s) 160, 237, 349, 367, 454, 613, 673, 724
SfaNI GCATC 2 cut(s) 422, 628
SinI GGWCC 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 315
SphI GCATGC 1 cut(s) 624
Sse9I AATT 2 cut(s) 14, 321
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 5 cut(s) 206, 255, 754, 808, 829
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 3 cut(s) 84, 95, 522
StyI CCWWGG 2 cut(s) 199, 316
TaaI ACNGT 1 cut(s) 573
TaiI ACGT 1 cut(s) 349
TaqI TCGA 2 cut(s) 9, 666
TasI AATT 2 cut(s) 14, 321
TauI GCSGC 2 cut(s) 258, 811
TfiI GAWTC 1 cut(s) 275
TscAI CASTG 3 cut(s) 446, 578, 622
TseFI GTSAC 1 cut(s) 799
TseI GCWGC 3 cut(s) 518, 556, 811
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 4 cut(s) 192, 297, 357, 388
TspRI CASTG 3 cut(s) 446, 578, 622
VpaK11BI GGWCC 1 cut(s) 794
XceI RCATGY 2 cut(s) 332, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.