Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0013506

Physical Location & Seq

Genomic Coordinates Forward (+)

6873181 .. 6877353

Gene Structure 4173 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0086100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGGAAGACAAGAGAGCAGCACCCAACGACGTCGTTGTGCAGATTTCCGGCGCCGAAGAATCCACCGTTATGTTTTCGCCGCAGCCTTCCCGAACTGGGTCTCCTCAAAAAGGGTTCCAAGGCTCCAACTTTGAGCTAAAGGAGCTCAGAGTCGCCGCCGGCTCTGCTTCGCCGGAGATCACAACCACCACCGCCGGCAAGCCGCCGAGAATTCCAGGCGAGCTTCGCCGGGTTTCGCTTGCCAGGTCGGCGTTCTCCAAGCCCAAGTCGAGGTTCCAGGAGCCAACTTACGTTAATGAAGTCAAGGTTGCTGAAGAAATTCCCAAGAGAAAGTCGAGTGCAAATTCACCCAATGTGGCTTCACCAAGCAGTAAAGCCACAGCCACTACTCCCAGAGAGGGTGTAAGATCAAACCCTATAACGCCGAGAACGCCGTTGATCGGAACACCGGCGCGTGGAGAAGAGGATGAGGAAGATGATGAAGAGGTGTACAAGACAGCCAATCTCAAGGTGCACCAGAAATCGGGTAAGAAGCTCAAAAAGTTGGTGTTACTTGAGTGGACTGTGTTTGTGTGCATTGTTGGGTTTTTAGTTGCTTGCTTAACTGTTGATAAGTTGGAGAGTAAGAATATTTGGTCATTGAAACTGTGGAAATGGTGCGTGCTAGTGTTGGTTGTGTTGTGTGGTAGGTTGGTTACAGAATGGCTTATTAATATTTTGGTGTTCGTGATAGAAACGAATTACTTGCTTAAGAAGAAGGTTTTGTATTTTGTTTATGGGTTGAAGAGGAGTGTGCAGGTGTTTATTTGGTTGGGTTTGATTCTACTAGCATGGGGTTTGTTGTTTGATCATGGAGTGAAGAGGTCGAAGAACACGTCTAAGATTCTAGGTTATGTGACAAGGGGTTTGGCTTCTTGTGAGATTGGAGCAGCCATATGGCTGTTGAAGAATTTGTTAGTGAAGCTTTTAGCCACTTCTTTTCAGTCCACCAGGTTCTTTGATCGGATTAAAGAGTCGATCTTTCATCATTATGTTCTCCGCAGCCTTTCGGGGCCTCCATTGGTGGAGATGGCTAGAAGAAAATTTGGGAAGGAATCAAGTAGTGGCCGGTTGAGTTTCAAAAATCTGCCAAAAGCTAAAAATGATGGGAAGGAAGGGCCCAGAGAAGAAGTGATTGACGTAGAAAAACTTTCAAAAATGAAGCAGGATAAAATTTCTGCTTGGACGATGAAAGGGTTGATTAATGTGATAAGTGGTTCTGGGTTGTCTACCATCTCCCACACACTAGACGACATTAACGATGAGGAGGGTGAGCAGAGGGATATAGAGATTACTAGTGAGTTTGAAGCACAGGCTGCAGCTTATGACATTTTCCTGAATGTAGCCAAGCACGGTAGCAAGTACATTGAGGAAGATGACCTCTGTCGCTTCTTGAAAAAGGACGAAGTCGAGCTCGTATTTCCTCTGTTTGAAGGTGGAGCAGAAAGTCGAAAGATAAAAAAAAAGCCTTTGAAGAACTGGCTGGTGAATGTTTACAACGAACGCAAGTCATTGGCACATTCCTTAACTGATACTAAAACAGCTATAGAGGAGTTGAATAGGCTTGGCTCAGCAGTTGTGCTTGTTGTGATCCTTATCGTGTGGTTACTTCTGATGGGATTTTTGACAACCAACATACTGGTCTTTATATCATCACAGCTTTTACTGGTGGTGTTTGTTTTTGGTAACACTGCTAAAACTGTGTTTGAAGCCATCATATTTGTCTTTGTGATGCACCCATTTGATGTAGGGGACCGTTGTGTTGTTGATGGAGTACAGTTGATTGTTGAAGAGATGAACATATTGACAACGATCTTCTTGAAACCTGACAATGAGAAGATATACTATCCAAATTCAGTACTGGCTACTAAACCCATCAGCAACTTTTATAGGAGCCCAGAGATGGGTGATGCTGTTGAGTTCGCTGTTGATGTTTCTACAACAATTGACACTATTGCGAGCATGAAAGCCAGAATAAAAACATACTTGGAGAGCAAGCCTCAGCACTGGCGTCCTGCCCATAGTGTGGCGGTGAAGGAAATTGAAGATGTAAACAAGATGACAATGGCTCTTTATATTTCTCATACTATAAATTTCCAGAACTATGGAGACAAGACCAGCCGCCGAACAGACCTAATCTTAGAGCTGAAGAAAATATTTGAAGAGCTTAATATAAAATATCACCTCCTGCCTCAGGAGATTTATCTCACCAAAGTTGGGTCAGCAACTACAGAGCTTCCACCCTCATGGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000179 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008649 GO:0008757 GO:0009116 GO:0009119 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016433 GO:0016740 GO:0016741 GO:0017144 GO:0022613 GO:0031167 GO:0031974 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042278 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046085 GO:0046128 GO:0046483 GO:0055086 GO:0070013 GO:0071704 GO:0071840 GO:0072521 GO:0090304 GO:0140098 GO:0140102 GO:1901135 GO:1901360 GO:1901564 GO:1901657

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RrnaAD

Protein Analysis

764

Amino Acids

86.0

Weight (kDa)

9.08

Isoelectric Point (pI)

42.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	186 - 327	3.9e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	586 - 642	4e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013506)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36380 AT4G36380
fragaria_vesca	FvH4_1g12330
malus_domestica	MD02G1137700.v1.1 MD15G1251500.v1.1
prunus_persica	Prupe.7G162900_v2.0.a1
pyrus_communis	pycom02g10900 pycom15g22160
rosa_chinensis	RchiOBHm_Chr2g0100151
rosa_laevigata	RLG00000016941
rosa_multiflora	Rmu_sc0018554.1_g000005
rosa_roxburghii	Rroxscaffold_2G00142520
rosa_rugosa	Rorug02G0086100
rosa_samantha	Rh2AG135100 Rh2BG138500 Rh2CG140300 Rh2DG140500
rosa_wichuraiana	Rw2G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	787
AatII	GACGTC	1 cut(s)	33
Acc36I	ACCTGC	1 cut(s)	787
AccB1I	GGYRCC	1 cut(s)	50
AccB7I	CCANNNNNTGG	1 cut(s)	2065
AccI	GTMKAC	1 cut(s)	1268
AccII	CGCG	1 cut(s)	454
AciI	CCGC	7 cut(s)	80, 156, 192, 203, 1039, 2069, 2161
AclWI	GGATC	1 cut(s)	1624
AcoI	YGGCCR	1 cut(s)	1105
AcsI	RAATTY	8 cut(s)	210, 318, 343, 949, 1082, 1212, 1891, 2131
AcuI	CTGAAG	2 cut(s)	333, 2207
AcyI	GRCGYC	3 cut(s)	30, 51, 2050
AfaI	GTAC	4 cut(s)	491, 1403, 1815, 1899
AflII	CTTAAG	1 cut(s)	749
AflIII	ACRYGT	1 cut(s)	873
AhlI	ACTAGT	1 cut(s)	1334
AjiI	CACGTC	1 cut(s)	876
AjnI	CCWGG	4 cut(s)	214, 242, 276, 989
AjuI	GAANNNNNNNTTGG	2 cut(s)	257, 289
AloI	GAACNNNNNNTCC	2 cut(s)	85, 117
Alw21I	GWGCWC	3 cut(s)	147, 516, 1455
Alw26I	GTCTC	2 cut(s)	105, 2142
Alw44I	GTGCAC	1 cut(s)	512
AlwI	GGATC	1 cut(s)	1624
AoxI	GGCC	3 cut(s)	1052, 1105, 1157
ApaI	GGGCCC	1 cut(s)	1161
ApaLI	GTGCAC	1 cut(s)	512
ApeKI	GCWGC	6 cut(s)	17, 82, 929, 1041, 1355, 1358
ApoI	RAATTY	8 cut(s)	210, 318, 343, 949, 1082, 1212, 1891, 2131
ArsI	GACNNNNNNTTYG	4 cut(s)	860, 889, 892, 921
AseI	ATTAAT	2 cut(s)	711, 1242
Asp700I	GAANNNNTTC	1 cut(s)	318
AspLEI	GCGC	3 cut(s)	53, 454, 2292
AspS9I	GGNCC	4 cut(s)	1052, 1157, 1158, 1792
AsuC2I	CCSGG	1 cut(s)	230
AsuHPI	GGTGA	8 cut(s)	339, 354, 1322, 1537, 1958, 2083, 2213, 2239
AvaII	GGWCC	1 cut(s)	1792
AxyI	CCTNAGG	1 cut(s)	2232
BaeGI	GKGCMC	2 cut(s)	516, 1161
BanI	GGYRCC	1 cut(s)	50
BanII	GRGCYC	4 cut(s)	147, 1161, 1455, 1937
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	3 cut(s)	147, 516, 1455
BbvCI	CCTCAGC	1 cut(s)	2040
BbvI	GCAGC	6 cut(s)	29, 94, 941, 1053, 1342, 1370
BccI	CCATC	8 cut(s)	1063, 1139, 1280, 1648, 1760, 1802, 1922, 1936
BceAI	ACGGC	1 cut(s)	418
BcgI	CGANNNNNNTGC	2 cut(s)	727, 761
BciT130I	CCWGG	4 cut(s)	216, 244, 278, 991
BclI	TGATCA	1 cut(s)	847
BcnI	CCSGG	1 cut(s)	230
BcoDI	GTCTC	2 cut(s)	105, 2142
BcuI	ACTAGT	1 cut(s)	1334
BfaI	CTAG	6 cut(s)	665, 827, 887, 1074, 1286, 1335
BfmI	CTRYAG	3 cut(s)	1356, 1584, 2268
BfoI	RGCGCY	2 cut(s)	54, 2293
BfrI	CTTAAG	1 cut(s)	749
BfuAI	ACCTGC	1 cut(s)	787
BglI	GCCNNNNNGGC	1 cut(s)	248
BlpI	GCTNAGC	1 cut(s)	1609
BmcAI	AGTACT	1 cut(s)	1899
Bme1390I	CCNGG	5 cut(s)	216, 230, 244, 278, 991
Bme18I	GGWCC	1 cut(s)	1792
BmgBI	CACGTC	1 cut(s)	876
BmgT120I	GGNCC	4 cut(s)	1052, 1157, 1158, 1792
BmiI	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1159, 1793, 1934
BmrFI	CCNGG	5 cut(s)	216, 230, 244, 278, 991
BmrI	ACTGGG	1 cut(s)	105
BmsI	GCATC	2 cut(s)	1761, 1939
BmuI	ACTGGG	1 cut(s)	105
BoxI	GACNNNNGTC	1 cut(s)	1422
BpiI	GAAGAC	1 cut(s)	12
BplI	GAGNNNNNCTC	2 cut(s)	2023, 2055
Bpu10I	CCTNAGC	1 cut(s)	2040
Bpu1102I	GCTNAGC	1 cut(s)	1609
BpuEI	CTTGAG	2 cut(s)	491, 575
BpuMI	CCSGG	1 cut(s)	230
BsaBI	GATNNNNATC	1 cut(s)	1634
BsaHI	GRCGYC	3 cut(s)	30, 51, 2050
BsaI	GGTCTC	1 cut(s)	105
BsaJI	CCNNGG	1 cut(s)	118
BsaXI	ACNNNNNCTCC	2 cut(s)	85, 115
Bse118I	RCCGGY	4 cut(s)	158, 194, 448, 1107
Bse1I	ACTGG	6 cut(s)	100, 1523, 1683, 1710, 1905, 2051
Bse21I	CCTNAGG	1 cut(s)	2232
Bse8I	GATNNNNATC	1 cut(s)	1634
BseBI	CCWGG	4 cut(s)	216, 244, 278, 991
BseDI	CCNNGG	1 cut(s)	118
BseGI	GGATG	1 cut(s)	472
BseJI	GATNNNNATC	1 cut(s)	1634
BseMII	CTCAG	4 cut(s)	160, 1623, 2054, 2246
BseNI	ACTGG	6 cut(s)	100, 1523, 1683, 1710, 1905, 2051
BseRI	GAGGAG	4 cut(s)	93, 802, 1319, 1604
BseSI	GKGCMC	2 cut(s)	516, 1161
BseXI	GCAGC	6 cut(s)	29, 94, 941, 1053, 1342, 1370
BsgI	GTGCAG	2 cut(s)	59, 815
Bsh1236I	CGCG	1 cut(s)	454
BshFI	GGCC	3 cut(s)	1054, 1107, 1159
BshNI	GGYRCC	1 cut(s)	50
BsiHKAI	GWGCWC	3 cut(s)	147, 516, 1455
BsiSI	CCGG	7 cut(s)	48, 159, 173, 195, 229, 449, 1108
BslFI	GGGAC	1 cut(s)	1805
BsmAI	GTCTC	2 cut(s)	105, 2142
BsmFI	GGGAC	1 cut(s)	1805
BsnI	GGCC	3 cut(s)	1054, 1107, 1159
Bso31I	GGTCTC	1 cut(s)	105
Bsp120I	GGGCCC	1 cut(s)	1157
Bsp1286I	GDGCHC	5 cut(s)	147, 516, 1161, 1455, 1937
Bsp1407I	TGTACA	1 cut(s)	489
Bsp143I	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
Bsp1720I	GCTNAGC	1 cut(s)	1609
BspACI	CCGC	7 cut(s)	80, 156, 192, 203, 1039, 2069, 2161
BspANI	GGCC	3 cut(s)	1054, 1107, 1159
BspCNI	CTCAG	4 cut(s)	159, 1622, 2053, 2245
BspFNI	CGCG	1 cut(s)	454
BspLI	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1159, 1793, 1934
BspMAI	CTGCAG	1 cut(s)	1360
BspMI	ACCTGC	1 cut(s)	787
BspPI	GGATC	1 cut(s)	1624
BspQI	GCTCTTC	1 cut(s)	2196
BspT107I	GGYRCC	1 cut(s)	50
BspTI	CTTAAG	1 cut(s)	749
BspTNI	GGTCTC	1 cut(s)	105
BsrFI	RCCGGY	4 cut(s)	158, 194, 448, 1107
BsrGI	TGTACA	1 cut(s)	489
BsrI	ACTGG	6 cut(s)	100, 1523, 1683, 1710, 1905, 2051
BssAI	RCCGGY	4 cut(s)	158, 194, 448, 1107
BssECI	CCNNGG	1 cut(s)	118
BssMI	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
BssNI	GRCGYC	3 cut(s)	30, 51, 2050
BssT1I	CCWWGG	1 cut(s)	118
Bst2UI	CCWGG	4 cut(s)	216, 244, 278, 991
Bst4CI	ACNGT	8 cut(s)	67, 565, 607, 648, 1394, 1741, 1796, 1818
Bst6I	CTCTTC	6 cut(s)	456, 477, 779, 854, 1824, 2196
BstACI	GRCGYC	3 cut(s)	30, 51, 2050
BstAFI	CTTAAG	1 cut(s)	749
BstAPI	GCANNNNNTGC	1 cut(s)	1355
BstAUI	TGTACA	1 cut(s)	489
BstC8I	GCNNGC	9 cut(s)	160, 196, 200, 221, 240, 598, 662, 1999, 2036
BstDEI	CTNAG	6 cut(s)	146, 879, 1609, 2040, 2179, 2232
BstENI	CCTNNNNNAGG	2 cut(s)	108, 2231
BstF5I	GGATG	1 cut(s)	472
BstFNI	CGCG	1 cut(s)	454
BstH2I	RGCGCY	2 cut(s)	54, 2293
BstHHI	GCGC	3 cut(s)	53, 454, 2292
BstKTI	GATC	8 cut(s)	180, 410, 441, 850, 1003, 1020, 1632, 1854
BstMAI	GTCTC	2 cut(s)	105, 2142
BstMBI	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
BstMWI	GCNNNNNNNGC	6 cut(s)	142, 164, 225, 248, 430, 1355
BstNI	CCWGG	4 cut(s)	216, 244, 278, 991
BstPAI	GACNNNNGTC	1 cut(s)	1422
BstSCI	CCNGG	5 cut(s)	214, 228, 242, 276, 989
BstSFI	CTRYAG	3 cut(s)	1356, 1584, 2268
BstSLI	GKGCMC	2 cut(s)	516, 1161
BstUI	CGCG	1 cut(s)	454
BstV1I	GCAGC	6 cut(s)	29, 94, 941, 1053, 1342, 1370
BstV2I	GAAGAC	1 cut(s)	12
BstXI	CCANNNNNNTGG	3 cut(s)	1678, 2144, 2286
Bsu36I	CCTNAGG	1 cut(s)	2232
BsuRI	GGCC	3 cut(s)	1054, 1107, 1159
BtrI	CACGTC	1 cut(s)	876
BtsCI	GGATG	1 cut(s)	472
BtsI	GCAGTG	1 cut(s)	1728
BtsIMutI	CAGTG	2 cut(s)	1728, 2044
BveI	ACCTGC	1 cut(s)	787
Cac8I	GCNNGC	9 cut(s)	160, 196, 200, 221, 240, 598, 662, 1999, 2036
CfoI	GCGC	3 cut(s)	53, 454, 2292
Cfr10I	RCCGGY	4 cut(s)	158, 194, 448, 1107
Cfr13I	GGNCC	4 cut(s)	1052, 1157, 1158, 1792
CseI	GACGC	1 cut(s)	2039
CsiI	ACCWGGT	1 cut(s)	989
Csp6I	GTAC	4 cut(s)	490, 1402, 1814, 1898
CviAII	CATG	4 cut(s)	831, 851, 2002, 2286
CviQI	GTAC	4 cut(s)	490, 1402, 1814, 1898
DdeI	CTNAG	6 cut(s)	146, 879, 1609, 2040, 2179, 2232
DinI	GGCGCC	1 cut(s)	52
DpnI	GATC	8 cut(s)	179, 409, 440, 849, 1002, 1019, 1631, 1853
DpnII	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
EaeI	YGGCCR	1 cut(s)	1105
Eam1104I	CTCTTC	6 cut(s)	456, 477, 779, 854, 1824, 2196
EarI	CTCTTC	6 cut(s)	456, 477, 779, 854, 1824, 2196
Ecl136II	GAGCTC	2 cut(s)	145, 1453
Eco130I	CCWWGG	1 cut(s)	118
Eco24I	GRGCYC	4 cut(s)	147, 1161, 1455, 1937
Eco31I	GGTCTC	1 cut(s)	105
Eco47I	GGWCC	1 cut(s)	1792
Eco53kI	GAGCTC	2 cut(s)	145, 1453
Eco57I	CTGAAG	2 cut(s)	333, 2207
Eco81I	CCTNAGG	1 cut(s)	2232
EcoICRI	GAGCTC	2 cut(s)	145, 1453
EcoNI	CCTNNNNNAGG	2 cut(s)	108, 2231
EcoO109I	RGGNCCY	2 cut(s)	1052, 1157
EcoRI	GAATTC	1 cut(s)	210
EcoRII	CCWGG	4 cut(s)	214, 242, 276, 989
EcoT14I	CCWWGG	1 cut(s)	118
EcoT38I	GRGCYC	4 cut(s)	147, 1161, 1455, 1937
EgeI	GGCGCC	1 cut(s)	52
EheI	GGCGCC	1 cut(s)	52
ErhI	CCWWGG	1 cut(s)	118
FaeI	CATG	4 cut(s)	834, 854, 2005, 2289
FaqI	GGGAC	1 cut(s)	1805
FatI	CATG	4 cut(s)	830, 850, 2001, 2285
FauNDI	CATATG	1 cut(s)	935
FbaI	TGATCA	1 cut(s)	847
FblI	GTMKAC	1 cut(s)	1268
FokI	GGATG	1 cut(s)	479
FriOI	GRGCYC	4 cut(s)	147, 1161, 1455, 1937
FspBI	CTAG	6 cut(s)	665, 827, 887, 1074, 1286, 1335
GlaI	GCGC	3 cut(s)	52, 453, 2291
HaeII	RGCGCY	2 cut(s)	54, 2293
HaeIII	GGCC	3 cut(s)	1054, 1107, 1159
HapII	CCGG	7 cut(s)	48, 159, 173, 195, 229, 449, 1108
HgaI	GACGC	1 cut(s)	2039
HhaI	GCGC	3 cut(s)	53, 454, 2292
Hin1I	GRCGYC	3 cut(s)	30, 51, 2050
Hin1II	CATG	4 cut(s)	834, 854, 2005, 2289
Hin6I	GCGC	3 cut(s)	51, 452, 2290
HinP1I	GCGC	3 cut(s)	51, 452, 2290
HindIII	AAGCTT	1 cut(s)	962
HinfI	GANTC	6 cut(s)	59, 150, 820, 883, 1013, 1094
HpaII	CCGG	7 cut(s)	48, 159, 173, 195, 229, 449, 1108
HphI	GGTGA	8 cut(s)	339, 354, 1322, 1537, 1958, 2083, 2213, 2239
Hpy166II	GTNNAC	7 cut(s)	490, 514, 561, 987, 1269, 1534, 2092
Hpy188I	TCNGA	4 cut(s)	149, 443, 1005, 1653
Hpy188III	TCNNGA	7 cut(s)	90, 727, 1375, 1432, 1858, 2137, 2234
Hpy8I	GTNNAC	7 cut(s)	490, 514, 561, 987, 1269, 1534, 2092
Hpy99I	CGWCG	2 cut(s)	32, 35
HpyAV	CCTTC	7 cut(s)	96, 751, 1084, 1144, 1148, 1466, 2068
HpyCH4III	ACNGT	8 cut(s)	67, 565, 607, 648, 1394, 1741, 1796, 1818
HpyCH4IV	ACGT	4 cut(s)	30, 291, 875, 1179
HpyCH4V	TGCA	7 cut(s)	40, 341, 514, 576, 796, 1358, 1774
HpyF10VI	GCNNNNNNNGC	6 cut(s)	142, 164, 225, 248, 430, 1355
HpyF3I	CTNAG	6 cut(s)	146, 879, 1609, 2040, 2179, 2232
HpySE526I	ACGT	4 cut(s)	30, 291, 875, 1179
Hsp92I	GRCGYC	3 cut(s)	30, 51, 2050
Hsp92II	CATG	4 cut(s)	834, 854, 2005, 2289
HspAI	GCGC	3 cut(s)	51, 452, 2290
KasI	GGCGCC	1 cut(s)	50
KroI	GCCGGC	2 cut(s)	158, 194
KroNI	GCCGGC	2 cut(s)	160, 196
Ksp22I	TGATCA	1 cut(s)	847
Kzo9I	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
LguI	GCTCTTC	1 cut(s)	2196
LmnI	GCTCC	6 cut(s)	128, 142, 280, 926, 1478, 1932
Lsp1109I	GCAGC	6 cut(s)	29, 94, 941, 1053, 1342, 1370
LweI	GCATC	2 cut(s)	1761, 1939
MabI	ACCWGGT	1 cut(s)	989
MaeI	CTAG	6 cut(s)	665, 827, 887, 1074, 1286, 1335
MaeII	ACGT	4 cut(s)	30, 291, 875, 1179
MaeIII	GTNAC	5 cut(s)	549, 694, 895, 1644, 1724
MalI	GATC	8 cut(s)	179, 409, 440, 849, 1002, 1019, 1631, 1853
MboI	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
MfeI	CAATTG	1 cut(s)	1983
MhlI	GDGCHC	5 cut(s)	147, 516, 1161, 1455, 1937
Mly113I	GGCGCC	1 cut(s)	51
MlyI	GAGTC	2 cut(s)	159, 1022
MmeI	TCCRAC	2 cut(s)	150, 597
MroNI	GCCGGC	2 cut(s)	158, 194
MroXI	GAANNNNTTC	1 cut(s)	318
MseI	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1242, 1296, 1565, 2208
MslI	CAYNNNNRTG	3 cut(s)	68, 1029, 2284
MspCI	CTTAAG	1 cut(s)	749
MspI	CCGG	7 cut(s)	48, 159, 173, 195, 229, 449, 1108
MspR9I	CCNGG	5 cut(s)	216, 230, 244, 278, 991
MunI	CAATTG	1 cut(s)	1983
MvaI	CCWGG	4 cut(s)	216, 244, 278, 991
MvnI	CGCG	1 cut(s)	454
MwoI	GCNNNNNNNGC	6 cut(s)	142, 164, 225, 248, 430, 1355
NaeI	GCCGGC	2 cut(s)	160, 196
NarI	GGCGCC	1 cut(s)	51
NciI	CCSGG	1 cut(s)	230
NdeI	CATATG	1 cut(s)	935
NdeII	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
NgoMIV	GCCGGC	2 cut(s)	158, 194
NlaIII	CATG	4 cut(s)	834, 854, 2005, 2289
NlaIV	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1159, 1793, 1934
NmeAIII	GCCGAG	2 cut(s)	231, 450
NmuCI	GTSAC	1 cut(s)	895
PaqCI	CACCTGC	1 cut(s)	787
PciSI	GCTCTTC	1 cut(s)	2196
PcsI	WCGNNNNNNNCGW	2 cut(s)	872, 1296
PdiI	GCCGGC	2 cut(s)	160, 196
PdmI	GAANNNNTTC	1 cut(s)	318
PfeI	GAWTC	4 cut(s)	59, 820, 883, 1094
PflFI	GACNNNGTC	1 cut(s)	1445
PflMI	CCANNNNNTGG	1 cut(s)	2065
PfoI	TCCNGGA	1 cut(s)	276
PleI	GAGTC	2 cut(s)	158, 1021
PluTI	GGCGCC	1 cut(s)	54
PpsI	GAGTC	2 cut(s)	158, 1021
PshAI	GACNNNNGTC	1 cut(s)	1422
PshBI	ATTAAT	2 cut(s)	711, 1242
Psp124BI	GAGCTC	2 cut(s)	147, 1455
Psp6I	CCWGG	4 cut(s)	214, 242, 276, 989
PspGI	CCWGG	4 cut(s)	214, 242, 276, 989
PspN4I	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1159, 1793, 1934
PspOMI	GGGCCC	1 cut(s)	1157
PspPI	GGNCC	4 cut(s)	1052, 1157, 1158, 1792
PstI	CTGCAG	1 cut(s)	1360
PsyI	GACNNNGTC	1 cut(s)	1445
RsaI	GTAC	4 cut(s)	491, 1403, 1815, 1899
RsaNI	GTAC	4 cut(s)	490, 1402, 1814, 1898
RseI	CAYNNNNRTG	3 cut(s)	68, 1029, 2284
SacI	GAGCTC	2 cut(s)	147, 1455
SapI	GCTCTTC	1 cut(s)	2196
SaqAI	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1242, 1296, 1565, 2208
Sau3AI	GATC	8 cut(s)	177, 407, 438, 847, 1000, 1017, 1629, 1851
Sau96I	GGNCC	4 cut(s)	1052, 1157, 1158, 1792
ScaI	AGTACT	1 cut(s)	1899
SchI	GAGTC	2 cut(s)	159, 1022
ScrFI	CCNGG	5 cut(s)	216, 230, 244, 278, 991
SduI	GDGCHC	5 cut(s)	147, 516, 1161, 1455, 1937
SexAI	ACCWGGT	1 cut(s)	989
SfaNI	GCATC	2 cut(s)	1761, 1939
SfcI	CTRYAG	3 cut(s)	1356, 1584, 2268
SfoI	GGCGCC	1 cut(s)	52
SgrAI	CRCCGGYG	1 cut(s)	448
SinI	GGWCC	1 cut(s)	1792
SmiMI	CAYNNNNRTG	3 cut(s)	68, 1029, 2284
SmlI	CTYRAG	3 cut(s)	506, 554, 749
SmoI	CTYRAG	3 cut(s)	506, 554, 749
SpeI	ACTAGT	1 cut(s)	1334
SsiI	CCGC	7 cut(s)	80, 156, 192, 203, 1039, 2069, 2161
SspDI	GGCGCC	1 cut(s)	50
SspI	AATATT	3 cut(s)	631, 715, 2196
SspMI	CTAG	6 cut(s)	665, 827, 887, 1074, 1286, 1335
SstI	GAGCTC	2 cut(s)	147, 1455
StyD4I	CCNGG	5 cut(s)	214, 228, 242, 276, 989
StyI	CCWWGG	1 cut(s)	118
TaaI	ACNGT	8 cut(s)	67, 565, 607, 648, 1394, 1741, 1796, 1818
TaiI	ACGT	4 cut(s)	33, 294, 878, 1182
TaqI	TCGA	6 cut(s)	269, 335, 866, 1016, 1449, 1489
TatI	WGTACW	4 cut(s)	489, 1401, 1813, 1897
TauI	GCSGC	4 cut(s)	82, 158, 205, 2163
TfiI	GAWTC	4 cut(s)	59, 820, 883, 1094
Tru1I	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1242, 1296, 1565, 2208
Tru9I	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1242, 1296, 1565, 2208
TscAI	CASTG	2 cut(s)	1735, 2051
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	6 cut(s)	17, 82, 929, 1041, 1355, 1358
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	7 cut(s)	312, 495, 1013, 1214, 1244, 1850, 2018
TspRI	CASTG	2 cut(s)	1735, 2051
Tth111I	GACNNNGTC	1 cut(s)	1445
Van91I	CCANNNNNTGG	1 cut(s)	2065
Vha464I	CTTAAG	1 cut(s)	749
VneI	GTGCAC	1 cut(s)	512
VpaK11BI	GGWCC	1 cut(s)	1792
VspI	ATTAAT	2 cut(s)	711, 1242
XagI	CCTNNNNNAGG	2 cut(s)	108, 2231
XapI	RAATTY	8 cut(s)	210, 318, 343, 949, 1082, 1212, 1891, 2131
XmiI	GTMKAC	1 cut(s)	1268
XmnI	GAANNNNTTC	1 cut(s)	318
XspI	CTAG	6 cut(s)	665, 827, 887, 1074, 1286, 1335
ZraI	GACGTC	1 cut(s)	31
ZrmI	AGTACT	1 cut(s)	1899

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.