Rorug02G0402700

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013476
Physical Location & Seq
Reverse (-)
51466843 .. 51467521
679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0402700.1

Sequence Viewer

Length: 474 bp
ATGGTCCTCACAACCATGGGCAGCATACGGAGCAATGATCCCCTCAGCATTTGGGAATTTGTCGGACTAAAACTCCATGACATGGTTTTAGATGGTTTGATTGAAGAGAAAAAATTGGACACATTTAATATGCCATACTATGCGCCAACAGCGGATGAGGTGAAAGAGGTGATCGAGGCTGAAGGTTCTTTTATTTTACAAAACCTCGAAACTTTTAGAAATGACTGGGACTGTTACATAAAACAAGTTAACTGTGGCTTTGACAAGAAAGCGAGGGCAGCAATACTATCCATTGACATAAGAGCTATGGGAGAGCCTATTCTGGCCAGCGAATTCGGAGAAAAAGCCATGGATGATTTGTTTTGCAGGTTTGAAGAAGATGTTCTTGATCACATGGAGAAGGAGAACTGCCAGTTCATTGACCTGGTTAATTATCTCGTTTGTTGGAAATTCTGGAGATGGATGATGCTTTGA

Protein Analysis

157

Amino Acids

18.4

Weight (kDa)

4.48

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 1 - 146 1.5e-32 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02065 AT3G02065 AT3G02065
fragaria_vesca FvH4_6g35850 FvH4_6g35850
malus_domestica MD17G1003800.v1.1
pyrus_communis pycom17g00050
rosa_chinensis RchiOBHm_Chr2g0147891
rosa_laevigata RLG00000020290
rosa_multiflora Rmu_sc0000308.1_g000058
rosa_roxburghii Rroxscaffold_2G00099220
rosa_rugosa Rorug02G0402700 Rorug02G0402800
rosa_samantha Rh2AG459700 Rh2BG473000 Rh2CG447000 Rh2DG481900
rosa_wichuraiana Rw2G037600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 82
AciI CCGC 1 cut(s) 152
AclWI GGATC 1 cut(s) 32
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 3 cut(s) 56, 332, 449
AcuI CTGAAG 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 2 cut(s) 104, 374
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 1 cut(s) 305
AluI AGCT 1 cut(s) 305
AlwI GGATC 1 cut(s) 32
AoxI GGCC 1 cut(s) 324
ApeKI GCWGC 2 cut(s) 21, 278
ApoI RAATTY 3 cut(s) 56, 332, 449
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 172, 181
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 326
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 2 cut(s) 33, 290
BccI CCATC 2 cut(s) 86, 453
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 2 cut(s) 22, 279
BlsI GCNGC 2 cut(s) 23, 280
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 425
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 15, 348
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 230, 412
Bse3DI GCAATG 1 cut(s) 40
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 15, 348
BseGI GGATG 3 cut(s) 160, 358, 468
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 2 cut(s) 230, 412
BseXI GCAGC 2 cut(s) 33, 290
BshFI GGCC 1 cut(s) 326
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 1 cut(s) 326
Bsp143I GATC 3 cut(s) 37, 171, 388
Bsp19I CCATGG 2 cut(s) 15, 348
BspACI CCGC 1 cut(s) 152
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 57
BspMI ACCTGC 1 cut(s) 357
BspPI GGATC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 40
BsrI ACTGG 2 cut(s) 230, 412
BssECI CCNNGG 2 cut(s) 15, 348
BssMI GATC 3 cut(s) 37, 171, 388
BssT1I CCWWGG 2 cut(s) 15, 348
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 2 cut(s) 233, 254
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 2 cut(s) 15, 348
BstF5I GGATG 3 cut(s) 160, 358, 468
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 3 cut(s) 40, 174, 391
BstMBI GATC 3 cut(s) 37, 171, 388
BstMWI GCNNNNNNNGC 3 cut(s) 30, 149, 278
BstNI CCWGG 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 423
BstV1I GCAGC 2 cut(s) 33, 290
BsuRI GGCC 1 cut(s) 326
BtgI CCRYGG 2 cut(s) 15, 348
BtsCI GGATG 3 cut(s) 160, 358, 468
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 328
CfoI GCGC 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 4
CsiI ACCWGGT 1 cut(s) 423
CviAII CATG 5 cut(s) 16, 77, 82, 349, 394
CviJI RGCY 6 cut(s) 179, 258, 305, 316, 326, 347
CviKI_1 RGCY 6 cut(s) 179, 258, 305, 316, 326, 347
DdeI CTNAG 1 cut(s) 44
DpnI GATC 3 cut(s) 39, 173, 390
DpnII GATC 3 cut(s) 37, 171, 388
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 15, 348
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 423
EcoT14I CCWWGG 2 cut(s) 15, 348
ErhI CCWWGG 2 cut(s) 15, 348
FaeI CATG 5 cut(s) 19, 80, 85, 352, 397
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 1 cut(s) 242
FatI CATG 5 cut(s) 15, 76, 81, 348, 393
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 22, 279
FokI GGATG 2 cut(s) 167, 365
Fsp4HI GCNGC 2 cut(s) 22, 279
GlaI GCGC 1 cut(s) 144
GluI GCNGC 2 cut(s) 22, 279
HaeIII GGCC 1 cut(s) 326
HhaI GCGC 1 cut(s) 145
Hin1II CATG 5 cut(s) 19, 80, 85, 352, 397
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HpaI GTTAAC 1 cut(s) 250
HphI GGTGA 2 cut(s) 172, 181
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 65, 338
Hpy188III TCNNGA 2 cut(s) 386, 454
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 176, 394
HpyCH4III ACNGT 2 cut(s) 233, 254
HpyCH4V TGCA 1 cut(s) 366
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 149, 278
HpyF3I CTNAG 1 cut(s) 44
Hsp92II CATG 5 cut(s) 19, 80, 85, 352, 397
HspAI GCGC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 388
KspAI GTTAAC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 37, 171, 388
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 8 cut(s) 211, 308, 340, 352, 410, 425, 437, 439
Lsp1109I GCAGC 2 cut(s) 33, 290
LweI GCATC 1 cut(s) 456
MabI ACCWGGT 1 cut(s) 423
MaeIII GTNAC 1 cut(s) 233
MalI GATC 3 cut(s) 39, 173, 390
MboI GATC 3 cut(s) 37, 171, 388
MboII GAAGA 3 cut(s) 116, 386, 389
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 5 cut(s) 56, 113, 332, 430, 449
MluNI TGGCCA 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 43, 425
MnlI CCTC 7 cut(s) 17, 53, 151, 160, 169, 215, 267
Mox20I TGGCCA 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 381
MscI TGGCCA 1 cut(s) 326
MseI TTAA 3 cut(s) 126, 249, 429
MslI CAYNNNNRTG 1 cut(s) 14
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 425
MwoI GCNNNNNNNGC 3 cut(s) 30, 149, 278
NcoI CCATGG 2 cut(s) 15, 348
NdeII GATC 3 cut(s) 37, 171, 388
NlaIII CATG 5 cut(s) 19, 80, 85, 352, 397
PdmI GAANNNNTTC 1 cut(s) 381
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 2 cut(s) 23, 280
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspPI GGNCC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 3 cut(s) 126, 249, 429
SatI GCNGC 2 cut(s) 22, 279
Sau3AI GATC 3 cut(s) 37, 171, 388
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 425
SetI ASST 7 cut(s) 162, 171, 187, 207, 307, 371, 426
SexAI ACCWGGT 1 cut(s) 423
SfaNI GCATC 1 cut(s) 456
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 5 cut(s) 56, 113, 332, 430, 449
SsiI CCGC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 15, 348
TaaI ACNGT 2 cut(s) 233, 254
TaqI TCGA 2 cut(s) 174, 207
TasI AATT 5 cut(s) 56, 113, 332, 430, 449
Tru1I TTAA 3 cut(s) 126, 249, 429
Tru9I TTAA 3 cut(s) 126, 249, 429
TseI GCWGC 2 cut(s) 21, 278
TspDTI ATGAA 1 cut(s) 406
TspGWI ACGGA 1 cut(s) 43
Van91I CCANNNNNTGG 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 3 cut(s) 56, 332, 449
XmnI GAANNNNTTC 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.