Rorug02G0537300

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018804
Physical Location & Seq
Forward (+)
66670637 .. 66672251
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0537300.1

Sequence Viewer

Length: 1074 bp
ATGGCGTCTTCTTCTTCTTCCTACTGCACCCGCTGGCACGCCATACTCTACTCCACCATGGCCGTCATCCTCGCCGCCATCGCAATCTCCATGACCCTCCGCTCCGACCCCACCGCCCAAACCCCCCAATCGGCCACACCCATCAGCCACGTCATCTCTCTCAGCGCCTCAAGAACTCTCCGGGCCTCCGGCTTCACCGTCACGGCGGCGCTCCTCCAAATCTCGCCGGAGCTCTTCCTCTCCTCGCCGAACTCCACCATCTTCGCAATCGCCGACTCCCACATCTCCAACGCCTCTCTCCCTCCTCACCTCCTCAGAGACCTCCTCAAATACCACACCTCGCCGGTTAGGGTTTCGATCGAAGACCTGATGGAGAAGCCTCAAGGTTCTTGCTTGCCGACTCTGTACCGGAAAAAGACCGTCACGGTCACGAGGATCGATCGGAAGAATCGGACGGTCCAGATCAACCGGGTTTGGGTCTCGCACCCGAATCTGTTTCTAGAAGGATCCGTTTCGATTCACGGCGTTCTTGGCCCGTTCGCGGCCGTGGATCCCGAATATGACGTCATTCACGCTCCTGTGTGCGACTCCAATTCTAGCGTCGTTTCTGGTTCTGGTGGATTGAAGAAGAGCAATGTGGAATGGGGTCGGATTATTAGGTTGCTGAGCTCAAAGGGGTTTGTGCCGTTTGCAATTGGGCTGCATTCTGTTCTTGATGGGATTCTTGAAGATGAGAGAAATGTGAGCTCTGTGACGATTTTTGTGCCACCAAGATTGGAGCTTGGAGCTCATCATCATCATCCTCGGGCTTTGCTTGAGAAGATTGTGAGGTTTCACATTGTGCCGCAGAGGCTTACACACCAGGAGCTTTCGGCATTGCCTGCGAGGACTCTGCTCAGGACTATGGTTCATGGTGATCAGCCTCTTGAAGTCACTGGGTTCATGAGTTTCATGCCGGAATTGGTCATCAATGGAGTGAGGATCGTGGCGCCCGACATCTATTCTTCGAACAACTTGGTTGTCCATGGGATTTCCCGAGCTTTCGAGGTGAATGATCTGCCTAATACCCAATAA

Protein Analysis

357

Amino Acids

39.08

Weight (kDa)

9.03

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 250 - 347 2.7e-07 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018804)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30440
rosa_chinensis RchiOBHm_Chr2g0169131
rosa_multiflora Rmu_ssc0000440.1_g000001
rosa_roxburghii Rroxscaffold_2G00082410
rosa_rugosa Rorug02G0537200 Rorug02G0537300
rosa_samantha Rh2CG590700 Rh2DG632600
rosa_wichuraiana Rw2G050510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 988
AccBSI CCGCTC 1 cut(s) 102
AccII CGCG 1 cut(s) 542
AciI CCGC 7 cut(s) 31, 75, 100, 114, 206, 542, 845
AclWI GGATC 6 cut(s) 443, 501, 514, 545, 558, 989
AcoI YGGCCR 3 cut(s) 60, 132, 543
AcyI GRCGYC 3 cut(s) 5, 564, 989
AdeI CACNNNGTG 1 cut(s) 841
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 3 cut(s) 130, 475, 541
AgsI TTSAA 3 cut(s) 625, 728, 929
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 861
AluBI AGCT 7 cut(s) 232, 669, 747, 781, 788, 868, 1040
AluI AGCT 7 cut(s) 232, 669, 747, 781, 788, 868, 1040
Alw21I GWGCWC 4 cut(s) 234, 671, 749, 790
Alw26I GTCTC 2 cut(s) 312, 484
AlwI GGATC 6 cut(s) 443, 501, 514, 545, 558, 989
Ama87I CYCGRG 2 cut(s) 804, 1035
AoxI GGCC 5 cut(s) 60, 132, 183, 532, 543
ApeKI GCWGC 1 cut(s) 700
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
AspLEI GCGC 3 cut(s) 167, 211, 991
AspS9I GGNCC 3 cut(s) 183, 457, 533
AsuC2I CCSGG 2 cut(s) 182, 470
AsuHPI GGTGA 4 cut(s) 187, 299, 926, 1060
AsuII TTCGAA 1 cut(s) 1007
AvaI CYCGRG 2 cut(s) 804, 1035
AvaII GGWCC 1 cut(s) 457
BamHI GGATCC 2 cut(s) 506, 550
BanI GGYRCC 1 cut(s) 988
BanII GRGCYC 4 cut(s) 234, 671, 749, 790
BauI CACGAG 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 4 cut(s) 234, 671, 749, 790
BbvI GCAGC 1 cut(s) 687
BccI CCATC 5 cut(s) 86, 149, 266, 364, 710
BceAI ACGGC 5 cut(s) 47, 219, 530, 538, 670
BcgI CGANNNNNNTGC 4 cut(s) 745, 779, 874, 908
BciT130I CCWGG 1 cut(s) 863
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 2 cut(s) 182, 470
BcoDI GTCTC 2 cut(s) 312, 484
BfaI CTAG 2 cut(s) 500, 597
BfoI RGCGCY 3 cut(s) 168, 212, 992
BglI GCCNNNNNGGC 1 cut(s) 850
BisI GCNGC 5 cut(s) 75, 207, 543, 701, 845
BlpI GCTNAGC 1 cut(s) 665
BlsI GCNGC 5 cut(s) 76, 208, 544, 702, 846
Bme1390I CCNGG 3 cut(s) 182, 470, 863
Bme18I GGWCC 1 cut(s) 457
BmeT110I CYCGRG 2 cut(s) 804, 1035
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 3 cut(s) 183, 457, 533
BmiI GGNNCC 3 cut(s) 508, 552, 990
BmrFI CCNGG 3 cut(s) 182, 470, 863
BmrI ACTGGG 1 cut(s) 945
BmuI ACTGGG 1 cut(s) 945
BpiI GAAGAC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 309, 341
Bpu10I CCTNAGC 1 cut(s) 896
Bpu1102I GCTNAGC 1 cut(s) 665
Bpu14I TTCGAA 1 cut(s) 1007
BpuEI CTTGAG 3 cut(s) 154, 366, 836
BpuMI CCSGG 2 cut(s) 182, 470
Bsa29I ATCGAT 1 cut(s) 438
BsaHI GRCGYC 3 cut(s) 5, 564, 989
BsaI GGTCTC 2 cut(s) 312, 484
BsaJI CCNNGG 4 cut(s) 57, 546, 803, 1024
BsaWI WCCGGW 1 cut(s) 408
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 475, 541
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 940
Bse3DI GCAATG 2 cut(s) 640, 875
BseBI CCWGG 1 cut(s) 863
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 4 cut(s) 57, 546, 803, 1024
BseGI GGATG 2 cut(s) 66, 799
BseLI CCNNNNNNNGG 3 cut(s) 130, 475, 541
BseMI GCAATG 2 cut(s) 640, 875
BseMII CTCAG 4 cut(s) 175, 328, 656, 910
BseNI ACTGG 1 cut(s) 940
BseRI GAGGAG 5 cut(s) 203, 232, 294, 302, 314
BseX3I CGGCCG 1 cut(s) 543
BseXI GCAGC 1 cut(s) 687
BsgI GTGCAG 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 542
Bsh1285I CGRYCG 3 cut(s) 360, 442, 546
BshFI GGCC 5 cut(s) 62, 134, 185, 534, 545
BshNI GGYRCC 1 cut(s) 988
BshVI ATCGAT 1 cut(s) 438
BsiEI CGRYCG 3 cut(s) 360, 442, 546
BsiHKAI GWGCWC 4 cut(s) 234, 671, 749, 790
BsiHKCI CYCGRG 2 cut(s) 804, 1035
BsiSI CCGG 7 cut(s) 181, 189, 227, 344, 409, 469, 956
BslI CCNNNNNNNGG 3 cut(s) 130, 475, 541
BsmAI GTCTC 2 cut(s) 312, 484
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 5 cut(s) 62, 134, 185, 534, 545
Bso31I GGTCTC 2 cut(s) 312, 484
BsoBI CYCGRG 2 cut(s) 804, 1035
Bsp119I TTCGAA 1 cut(s) 1007
Bsp1286I GDGCHC 4 cut(s) 234, 671, 749, 790
Bsp143I GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
Bsp1720I GCTNAGC 1 cut(s) 665
Bsp19I CCATGG 2 cut(s) 57, 1024
BspACI CCGC 7 cut(s) 31, 75, 100, 114, 206, 542, 845
BspANI GGCC 5 cut(s) 62, 134, 185, 534, 545
BspCNI CTCAG 4 cut(s) 174, 327, 657, 909
BspDI ATCGAT 1 cut(s) 438
BspFNI CGCG 1 cut(s) 542
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 3 cut(s) 508, 552, 990
BspPI GGATC 6 cut(s) 443, 501, 514, 545, 558, 989
BspQI GCTCTTC 2 cut(s) 239, 623
BspT104I TTCGAA 1 cut(s) 1007
BspT107I GGYRCC 1 cut(s) 988
BspTNI GGTCTC 2 cut(s) 312, 484
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 2 cut(s) 640, 875
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 1 cut(s) 940
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 4 cut(s) 57, 546, 803, 1024
BssMI GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
BssNI GRCGYC 3 cut(s) 5, 564, 989
BssSI CACGAG 1 cut(s) 430
BssT1I CCWWGG 2 cut(s) 57, 1024
Bst2BI CACGAG 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 863
Bst4CI ACNGT 4 cut(s) 199, 421, 427, 457
Bst6I CTCTTC 2 cut(s) 239, 623
BstACI GRCGYC 3 cut(s) 5, 564, 989
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBI TTCGAA 1 cut(s) 1007
BstC8I GCNNGC 4 cut(s) 35, 39, 395, 882
BstDEI CTNAG 4 cut(s) 161, 314, 665, 896
BstDSI CCRYGG 3 cut(s) 57, 546, 1024
BstF5I GGATG 2 cut(s) 66, 799
BstFNI CGCG 1 cut(s) 542
BstH2I RGCGCY 3 cut(s) 168, 212, 992
BstHHI GCGC 3 cut(s) 167, 211, 991
BstKTI GATC 9 cut(s) 360, 438, 442, 465, 509, 553, 919, 984, 1057
BstMAI GTCTC 2 cut(s) 312, 484
BstMBI GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
BstMCI CGRYCG 3 cut(s) 360, 442, 546
BstMWI GCNNNNNNNGC 4 cut(s) 80, 531, 850, 881
BstNI CCWGG 1 cut(s) 863
BstSCI CCNGG 3 cut(s) 180, 468, 861
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 1 cut(s) 687
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 2 cut(s) 506, 550
BstYI RGATCY 2 cut(s) 506, 550
BstZI CGGCCG 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 438
BsuRI GGCC 5 cut(s) 62, 134, 185, 534, 545
BsuTUI ATCGAT 1 cut(s) 438
BtgI CCRYGG 3 cut(s) 57, 546, 1024
BtgZI GCGATG 1 cut(s) 64
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 2 cut(s) 66, 799
BtsIMutI CAGTG 1 cut(s) 933
Cac8I GCNNGC 4 cut(s) 35, 39, 395, 882
CciI TCATGA 1 cut(s) 942
CfoI GCGC 3 cut(s) 167, 211, 991
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 3 cut(s) 183, 457, 533
ClaI ATCGAT 1 cut(s) 438
CseI GACGC 1 cut(s) 589
Csp6I GTAC 1 cut(s) 406
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 6 cut(s) 58, 91, 911, 943, 952, 1025
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 4 cut(s) 161, 314, 665, 896
DinI GGCGCC 1 cut(s) 990
DpnI GATC 9 cut(s) 359, 437, 441, 464, 508, 552, 918, 983, 1056
DpnII GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
DraIII CACNNNGTG 1 cut(s) 841
EaeI YGGCCR 3 cut(s) 60, 132, 543
EagI CGGCCG 1 cut(s) 543
Eam1104I CTCTTC 2 cut(s) 239, 623
EarI CTCTTC 2 cut(s) 239, 623
Ecl136II GAGCTC 4 cut(s) 232, 669, 747, 788
EclXI CGGCCG 1 cut(s) 543
Eco130I CCWWGG 2 cut(s) 57, 1024
Eco24I GRGCYC 4 cut(s) 234, 671, 749, 790
Eco31I GGTCTC 2 cut(s) 312, 484
Eco47I GGWCC 1 cut(s) 457
Eco52I CGGCCG 1 cut(s) 543
Eco53kI GAGCTC 4 cut(s) 232, 669, 747, 788
Eco88I CYCGRG 2 cut(s) 804, 1035
EcoICRI GAGCTC 4 cut(s) 232, 669, 747, 788
EcoRII CCWGG 1 cut(s) 861
EcoT14I CCWWGG 2 cut(s) 57, 1024
EcoT38I GRGCYC 4 cut(s) 234, 671, 749, 790
EgeI GGCGCC 1 cut(s) 990
EheI GGCGCC 1 cut(s) 990
ErhI CCWWGG 2 cut(s) 57, 1024
FaeI CATG 6 cut(s) 61, 94, 914, 946, 955, 1028
FaiI YATR 9 cut(s) 44, 59, 92, 561, 905, 912, 944, 953, 1026
FatI CATG 6 cut(s) 57, 90, 910, 942, 951, 1024
FauI CCCGC 1 cut(s) 38
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 5 cut(s) 75, 207, 543, 701, 845
FokI GGATG 2 cut(s) 53, 786
FriOI GRGCYC 4 cut(s) 234, 671, 749, 790
Fsp4HI GCNGC 5 cut(s) 75, 207, 543, 701, 845
FspBI CTAG 2 cut(s) 500, 597
GlaI GCGC 3 cut(s) 166, 210, 990
GluI GCNGC 5 cut(s) 75, 207, 543, 701, 845
HaeII RGCGCY 3 cut(s) 168, 212, 992
HaeIII GGCC 5 cut(s) 62, 134, 185, 534, 545
HapII CCGG 7 cut(s) 181, 189, 227, 344, 409, 469, 956
HgaI GACGC 1 cut(s) 589
HhaI GCGC 3 cut(s) 167, 211, 991
Hin1I GRCGYC 3 cut(s) 5, 564, 989
Hin1II CATG 6 cut(s) 61, 94, 914, 946, 955, 1028
Hin6I GCGC 3 cut(s) 165, 209, 989
HinP1I GCGC 3 cut(s) 165, 209, 989
HinfI GANTC 8 cut(s) 275, 400, 448, 490, 517, 587, 721, 889
HpaII CCGG 7 cut(s) 181, 189, 227, 344, 409, 469, 956
HphI GGTGA 4 cut(s) 187, 299, 926, 1060
Hpy188I TCNGA 5 cut(s) 106, 317, 444, 453, 651
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 4 cut(s) 199, 421, 427, 457
HpyCH4IV ACGT 2 cut(s) 150, 564
HpyCH4V TGCA 3 cut(s) 27, 692, 703
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 531, 850, 881
HpyF3I CTNAG 4 cut(s) 161, 314, 665, 896
HpySE526I ACGT 2 cut(s) 150, 564
Hsp92I GRCGYC 3 cut(s) 5, 564, 989
Hsp92II CATG 6 cut(s) 61, 94, 914, 946, 955, 1028
HspAI GCGC 3 cut(s) 165, 209, 989
KasI GGCGCC 1 cut(s) 988
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
LguI GCTCTTC 2 cut(s) 239, 623
LmnI GCTCC 7 cut(s) 107, 216, 229, 580, 778, 785, 865
Lsp1109I GCAGC 1 cut(s) 687
MaeI CTAG 2 cut(s) 500, 597
MaeII ACGT 2 cut(s) 150, 564
MaeIII GTNAC 5 cut(s) 199, 421, 427, 751, 931
MalI GATC 9 cut(s) 359, 437, 441, 464, 508, 552, 918, 983, 1056
MbiI CCGCTC 1 cut(s) 102
MboI GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 2 cut(s) 506, 550
MhlI GDGCHC 4 cut(s) 234, 671, 749, 790
MluCI AATT 3 cut(s) 592, 693, 959
Mly113I GGCGCC 1 cut(s) 989
MlyI GAGTC 4 cut(s) 269, 394, 581, 883
MmeI TCCRAC 3 cut(s) 129, 312, 629
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 7 cut(s) 181, 189, 227, 344, 409, 469, 956
MspR9I CCNGG 3 cut(s) 182, 470, 863
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 1 cut(s) 863
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 4 cut(s) 80, 531, 850, 881
NarI GGCGCC 1 cut(s) 989
NciI CCSGG 2 cut(s) 182, 470
NcoI CCATGG 2 cut(s) 57, 1024
NdeII GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
NlaIII CATG 6 cut(s) 61, 94, 914, 946, 955, 1028
NlaIV GGNNCC 3 cut(s) 508, 552, 990
NmuCI GTSAC 5 cut(s) 199, 421, 427, 751, 931
NspV TTCGAA 1 cut(s) 1007
PagI TCATGA 1 cut(s) 942
PciSI GCTCTTC 2 cut(s) 239, 623
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 990
PctI GAATGC 1 cut(s) 703
PfeI GAWTC 4 cut(s) 448, 490, 517, 721
PkrI GCNGC 5 cut(s) 76, 208, 544, 702, 846
Ple19I CGATCG 2 cut(s) 360, 442
PleI GAGTC 4 cut(s) 269, 394, 581, 883
PluTI GGCGCC 1 cut(s) 992
PpsI GAGTC 4 cut(s) 269, 394, 581, 883
Psp124BI GAGCTC 4 cut(s) 234, 671, 749, 790
Psp6I CCWGG 1 cut(s) 861
PspGI CCWGG 1 cut(s) 861
PspN4I GGNNCC 3 cut(s) 508, 552, 990
PspPI GGNCC 3 cut(s) 183, 457, 533
PsuI RGATCY 2 cut(s) 506, 550
PvuI CGATCG 2 cut(s) 360, 442
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SacI GAGCTC 4 cut(s) 234, 671, 749, 790
SapI GCTCTTC 2 cut(s) 239, 623
SatI GCNGC 5 cut(s) 75, 207, 543, 701, 845
Sau3AI GATC 9 cut(s) 357, 435, 439, 462, 506, 550, 916, 981, 1054
Sau96I GGNCC 3 cut(s) 183, 457, 533
SchI GAGTC 4 cut(s) 269, 394, 581, 883
ScrFI CCNGG 3 cut(s) 182, 470, 863
SduI GDGCHC 4 cut(s) 234, 671, 749, 790
SfoI GGCGCC 1 cut(s) 990
SfuI TTCGAA 1 cut(s) 1007
SinI GGWCC 1 cut(s) 457
SmlI CTYRAG 3 cut(s) 169, 381, 815
SmoI CTYRAG 3 cut(s) 169, 381, 815
Sse9I AATT 3 cut(s) 592, 693, 959
SsiI CCGC 7 cut(s) 31, 75, 100, 114, 206, 542, 845
SspDI GGCGCC 1 cut(s) 988
SspMI CTAG 2 cut(s) 500, 597
SstI GAGCTC 4 cut(s) 234, 671, 749, 790
StyD4I CCNGG 3 cut(s) 180, 468, 861
StyI CCWWGG 2 cut(s) 57, 1024
TaaI ACNGT 4 cut(s) 199, 421, 427, 457
TaiI ACGT 2 cut(s) 153, 567
TaqI TCGA 6 cut(s) 356, 360, 438, 515, 1007, 1044
TasI AATT 3 cut(s) 592, 693, 959
TauI GCSGC 4 cut(s) 77, 209, 545, 847
TfiI GAWTC 4 cut(s) 448, 490, 517, 721
TscAI CASTG 1 cut(s) 940
TseFI GTSAC 5 cut(s) 199, 421, 427, 751, 931
TseI GCWGC 1 cut(s) 700
Tsp45I GTSAC 5 cut(s) 199, 421, 427, 751, 931
TspDTI ATGAA 3 cut(s) 899, 931, 940
TspGWI ACGGA 1 cut(s) 499
TspRI CASTG 1 cut(s) 940
VpaK11BI GGWCC 1 cut(s) 457
XbaI TCTAGA 1 cut(s) 499
XspI CTAG 2 cut(s) 500, 597
ZraI GACGTC 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.