Rorug02G0589600

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011084
Physical Location & Seq
Reverse (-)
71159417 .. 71161133
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0589600.1

Sequence Viewer

Length: 327 bp
ATGGCCACAGTCAAGTTTGTTGCTGCTTTGATCTTGTCACTCCTTGCAATCTCCATGCTCCAAACCATGGTTATGGCAAACCATGGCCATGGAGGTCACCACAAAGGCAATAATCAATATGGACCAGGGAGTCTCAAGAGCTACCAATGCCCATCACAGTGCACAAGGAGGTGCAGCCAGACCCAATACCACAAGCCCTGCATGTTTTTCTGCCAGAAATGCTGCAGCAAGTGCCTGTGTGTGCCTCCAGGGTATTATGGAAACAAGGCTGTGTGCCCTTGCTACAACAACTGGAAGACCAAGGAAGGAGGACCAAAATGCCCTTGA

Protein Analysis

108

Amino Acids

11.91

Weight (kDa)

9.24

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GASA PF02704 49 - 108 5.8e-23 Gibberellin regulated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 67
AcoI YGGCCR 2 cut(s) 3, 85
AfiI CCNNNNNNNGG 1 cut(s) 67
AjnI CCWGG 2 cut(s) 124, 247
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 137
Alw44I GTGCAC 1 cut(s) 160
AoxI GGCC 2 cut(s) 3, 85
ApaLI GTGCAC 1 cut(s) 160
ApeKI GCWGC 4 cut(s) 23, 174, 222, 225
AspS9I GGNCC 2 cut(s) 122, 311
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 2 cut(s) 122, 311
BaeGI GKGCMC 2 cut(s) 164, 278
BalI TGGCCA 2 cut(s) 5, 87
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 4 cut(s) 10, 186, 209, 237
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 2 cut(s) 126, 249
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 223
BisI GCNGC 4 cut(s) 24, 175, 223, 226
BlsI GCNGC 4 cut(s) 25, 176, 224, 227
Bme1390I CCNGG 2 cut(s) 126, 249
Bme18I GGWCC 2 cut(s) 122, 311
BmgT120I GGNCC 2 cut(s) 122, 311
BmrFI CCNGG 2 cut(s) 126, 249
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 119
BsaJI CCNNGG 6 cut(s) 66, 82, 88, 125, 248, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 296
BseBI CCWGG 2 cut(s) 126, 249
BseDI CCNNGG 6 cut(s) 66, 82, 88, 125, 248, 300
BseLI CCNNNNNNNGG 1 cut(s) 67
BseNI ACTGG 1 cut(s) 296
BseSI GKGCMC 2 cut(s) 164, 278
BseXI GCAGC 4 cut(s) 10, 186, 209, 237
BsgI GTGCAG 1 cut(s) 193
BshFI GGCC 2 cut(s) 5, 87
BsiHKAI GWGCWC 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 2 cut(s) 5, 87
Bsp1286I GDGCHC 2 cut(s) 164, 278
Bsp143I GATC 1 cut(s) 30
Bsp19I CCATGG 3 cut(s) 66, 82, 88
BspANI GGCC 2 cut(s) 5, 87
BspMAI CTGCAG 1 cut(s) 227
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 6 cut(s) 66, 82, 88, 125, 248, 300
BssMI GATC 1 cut(s) 30
BssT1I CCWWGG 4 cut(s) 66, 82, 88, 300
Bst2UI CCWGG 2 cut(s) 126, 249
Bst4CI ACNGT 2 cut(s) 10, 159
BstAPI GCANNNNNTGC 1 cut(s) 231
BstDSI CCRYGG 3 cut(s) 66, 82, 88
BstEII GGTNACC 1 cut(s) 95
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 147, 219, 231
BstNI CCWGG 2 cut(s) 126, 249
BstNSI RCATGY 1 cut(s) 205
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 124, 247
BstSFI CTRYAG 1 cut(s) 223
BstSLI GKGCMC 2 cut(s) 164, 278
BstV1I GCAGC 4 cut(s) 10, 186, 209, 237
BstV2I GAAGAC 1 cut(s) 302
BstXI CCANNNNNNTGG 2 cut(s) 73, 89
BsuRI GGCC 2 cut(s) 5, 87
BtgI CCRYGG 3 cut(s) 66, 82, 88
BtsIMutI CAGTG 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 122, 311
CviAII CATG 5 cut(s) 55, 67, 83, 89, 202
CviJI RGCY 6 cut(s) 5, 87, 141, 177, 196, 269
CviKI_1 RGCY 6 cut(s) 5, 87, 141, 177, 196, 269
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
DrdI GACNNNNNNGTC 1 cut(s) 129
DseDI GACNNNNNNGTC 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 3, 85
Eco130I CCWWGG 4 cut(s) 66, 82, 88, 300
Eco47I GGWCC 2 cut(s) 122, 311
Eco91I GGTNACC 1 cut(s) 95
EcoO65I GGTNACC 1 cut(s) 95
EcoRII CCWGG 2 cut(s) 124, 247
EcoT14I CCWWGG 4 cut(s) 66, 82, 88, 300
ErhI CCWWGG 4 cut(s) 66, 82, 88, 300
FaeI CATG 5 cut(s) 58, 70, 86, 92, 205
FaiI YATR 8 cut(s) 56, 68, 74, 84, 90, 120, 203, 258
FatI CATG 5 cut(s) 54, 66, 82, 88, 201
Fnu4HI GCNGC 4 cut(s) 24, 175, 223, 226
Fsp4HI GCNGC 4 cut(s) 24, 175, 223, 226
GluI GCNGC 4 cut(s) 24, 175, 223, 226
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 2 cut(s) 5, 87
Hin1II CATG 5 cut(s) 58, 70, 86, 92, 205
HinfI GANTC 1 cut(s) 130
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 162
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 2 cut(s) 10, 159
HpyCH4V TGCA 5 cut(s) 47, 162, 174, 201, 225
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 219, 231
Hsp92II CATG 5 cut(s) 58, 70, 86, 92, 205
Kzo9I GATC 1 cut(s) 30
LmnI GCTCC 1 cut(s) 63
LpnPI CCDG 9 cut(s) 111, 138, 191, 211, 227, 234, 248, 261, 277
Lsp1109I GCAGC 4 cut(s) 10, 186, 209, 237
MaeIII GTNAC 2 cut(s) 36, 95
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 164, 278
MlsI TGGCCA 2 cut(s) 5, 87
MluNI TGGCCA 2 cut(s) 5, 87
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 4 cut(s) 86, 162, 255, 302
Mox20I TGGCCA 2 cut(s) 5, 87
MscI TGGCCA 2 cut(s) 5, 87
MslI CAYNNNNRTG 3 cut(s) 71, 87, 157
Msp20I TGGCCA 2 cut(s) 5, 87
MspR9I CCNGG 2 cut(s) 126, 249
MvaI CCWGG 2 cut(s) 126, 249
MwoI GCNNNNNNNGC 3 cut(s) 147, 219, 231
NcoI CCATGG 3 cut(s) 66, 82, 88
NdeII GATC 1 cut(s) 30
NlaIII CATG 5 cut(s) 58, 70, 86, 92, 205
NmuCI GTSAC 2 cut(s) 36, 95
NspI RCATGY 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 67
PkrI GCNGC 4 cut(s) 25, 176, 224, 227
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 124, 247
PspEI GGTNACC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 124, 247
PspPI GGNCC 2 cut(s) 122, 311
PstI CTGCAG 1 cut(s) 227
RseI CAYNNNNRTG 3 cut(s) 71, 87, 157
SatI GCNGC 4 cut(s) 24, 175, 223, 226
Sau3AI GATC 1 cut(s) 30
Sau96I GGNCC 2 cut(s) 122, 311
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 2 cut(s) 126, 249
SduI GDGCHC 2 cut(s) 164, 278
SetI ASST 3 cut(s) 97, 143, 173
SfcI CTRYAG 1 cut(s) 223
SinI GGWCC 2 cut(s) 122, 311
SmiMI CAYNNNNRTG 3 cut(s) 71, 87, 157
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 124, 247
StyI CCWWGG 4 cut(s) 66, 82, 88, 300
TaaI ACNGT 2 cut(s) 10, 159
TscAI CASTG 1 cut(s) 164
TseFI GTSAC 2 cut(s) 36, 95
TseI GCWGC 4 cut(s) 23, 174, 222, 225
Tsp45I GTSAC 2 cut(s) 36, 95
TspRI CASTG 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 67
VneI GTGCAC 1 cut(s) 160
VpaK11BI GGWCC 2 cut(s) 122, 311
XceI RCATGY 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.