Rh2CG643100

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011084
Physical Location & Seq
Forward (+)
81299858 .. 81304423
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG643100.1

Sequence Viewer

Length: 942 bp
ATGAACAACCGCAAAGTCGTCGTCTGCGACAACGGCACCGGATATGTGAAATGTGGGTTTGCCGGAGAGAACTTCCCTACATCTGTGTTCCCCTGCGTAGTTGGAAGGCCGTTGCTTCGCTACGAGGAATCACTCATGGAACAAGAAATCAAGGATATTATTGTGGGGGAAGCTTGCGCAGAGTTTCGACACCAATTGGAGATAAACTACCCTGTTAACAATGGCATTGTGCAGAACTGGGATGATATGGGTCATGTCTGGGACCATGCATTCTATAGTGAGCTCAAAATAGATCCTACTGAATGCCATATATTACTCACAGACCCTCCTCTCAATCCCTTGAAGAACCGCGAAAAGATGATCGAGACCATGTTTGAGAAGTACAATTTTGCTGGCGTCTTCATTCAAATTCAAGCTGTTTTAACACTGTATGCTCAAGGATTGCTGACTGGATTAGTTATTGACTCTGGTGATGGCGTTACTCATGTGGTTCCAGTTGTTGATGGCTACTCATTTCCTCATCTTACAAAGCGAATGAATGTAGCTGGCCGGCACATAACATCCTATCTTGTTGATTTGCTCTCACGGAGGGGGTATGCAATGAATAGGACGGCTGATTTTGAGAGTGTTAGGGAAATCAAAGAGAAGCTCTGCTATATAAGTTATGATTACAAAAGGGAGTATCAGTTAGGACTGGAGACAACCATACTTGTAAAGAATTACACTCTGCCAGATGGAAGGGTGATCAAAGTCGGCACTGAAAGGTTCCAGGCTCCCGAGGCTCTTTTCACACCTGAACTTATAGATGTTGAAGGTGATGGGATGGCTGACATGGTGTTCCGCTGCATTCAGGAAATGGATATTGACAATAGGATGATGCTTTACCAGCATATAGTTTTAAGTGGAGGGAGCACGATGTATCCTGGATTACCCAGTCGGTAA

Protein Analysis

313

Amino Acids

35.63

Weight (kDa)

5.17

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 4 - 313 6e-84 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 35
AccII CGCG 1 cut(s) 351
AciI CCGC 3 cut(s) 10, 349, 841
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 1 cut(s) 383
AgsI TTSAA 4 cut(s) 343, 407, 413, 812
AjnI CCWGG 2 cut(s) 768, 922
AluBI AGCT 5 cut(s) 173, 283, 416, 545, 649
AluI AGCT 5 cut(s) 173, 283, 416, 545, 649
Alw21I GWGCWC 2 cut(s) 285, 914
Alw26I GTCTC 2 cut(s) 359, 692
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 776
AoxI GGCC 2 cut(s) 107, 547
ApeKI GCWGC 1 cut(s) 843
ApoI RAATTY 1 cut(s) 408
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 3 cut(s) 482, 754, 827
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 1 cut(s) 262
BanI GGYRCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 2 cut(s) 285, 914
BbvI GCAGC 1 cut(s) 830
BccI CCATC 5 cut(s) 467, 497, 728, 812, 817
BceAI ACGGC 3 cut(s) 49, 94, 627
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 2 cut(s) 770, 924
BciVI GTATCC 1 cut(s) 930
BclI TGATCA 1 cut(s) 744
BcoDI GTCTC 2 cut(s) 359, 692
BfmI CTRYAG 1 cut(s) 274
BfuI GTATCC 1 cut(s) 930
BisI GCNGC 1 cut(s) 844
BlsI GCNGC 1 cut(s) 845
Bme1390I CCNGG 2 cut(s) 770, 924
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 776
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 5 cut(s) 37, 263, 492, 767, 774
BmrFI CCNGG 2 cut(s) 770, 924
BmrI ACTGGG 2 cut(s) 247, 927
BmsI GCATC 1 cut(s) 867
BmuI ACTGGG 2 cut(s) 247, 927
BpiI GAAGAC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 777
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 4 cut(s) 310, 340, 671, 701
Bse118I RCCGGY 1 cut(s) 549
Bse1I ACTGG 5 cut(s) 242, 454, 494, 699, 933
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 2 cut(s) 770, 924
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 4 cut(s) 247, 560, 828, 879
BseMI GCAATG 1 cut(s) 606
BseNI ACTGG 5 cut(s) 242, 454, 494, 699, 933
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 830
BsgI GTGCAG 1 cut(s) 251
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 109, 549
BshNI GGYRCC 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 285, 914
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 3 cut(s) 39, 63, 550
BslFI GGGAC 1 cut(s) 275
BsmAI GTCTC 2 cut(s) 359, 692
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 3 cut(s) 269, 308, 846
BsnI GGCC 2 cut(s) 109, 549
Bso31I GGTCTC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 2 cut(s) 285, 914
Bsp143I GATC 3 cut(s) 292, 360, 744
BspACI CCGC 3 cut(s) 10, 349, 841
BspANI GGCC 2 cut(s) 109, 549
BspFNI CGCG 1 cut(s) 351
BspLI GGNNCC 5 cut(s) 37, 263, 492, 767, 774
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 549
BsrI ACTGG 5 cut(s) 242, 454, 494, 699, 933
BssAI RCCGGY 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 3 cut(s) 292, 360, 744
BssNI GRCGYC 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 770, 924
Bst4CI ACNGT 1 cut(s) 429
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 4 cut(s) 175, 394, 547, 551
BstF5I GGATG 4 cut(s) 247, 560, 828, 879
BstFNI CGCG 1 cut(s) 351
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 3 cut(s) 295, 363, 747
BstMAI GTCTC 2 cut(s) 359, 692
BstMBI GATC 3 cut(s) 292, 360, 744
BstMWI GCNNNNNNNGC 3 cut(s) 33, 779, 886
BstNI CCWGG 2 cut(s) 770, 924
BstSCI CCNGG 2 cut(s) 768, 922
BstSFI CTRYAG 1 cut(s) 274
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 830
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 2 cut(s) 109, 549
BtsCI GGATG 4 cut(s) 247, 560, 828, 879
BtsIMutI CAGTG 2 cut(s) 425, 756
Cac8I GCNNGC 4 cut(s) 175, 394, 547, 551
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 1 cut(s) 549
Cfr13I GGNCC 1 cut(s) 262
CseI GACGC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 382
CviAII CATG 6 cut(s) 136, 254, 266, 370, 485, 832
CviQI GTAC 1 cut(s) 382
DpnI GATC 3 cut(s) 294, 362, 746
DpnII GATC 3 cut(s) 292, 360, 744
EaeI YGGCCR 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 262
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 768, 922
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 285
FaeI CATG 6 cut(s) 139, 257, 269, 373, 488, 835
FaqI GGGAC 1 cut(s) 275
FatI CATG 6 cut(s) 135, 253, 265, 369, 484, 831
FbaI TGATCA 1 cut(s) 744
Fnu4HI GCNGC 1 cut(s) 844
FokI GGATG 4 cut(s) 254, 547, 835, 886
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 1 cut(s) 844
FspI TGCGCA 1 cut(s) 178
GlaI GCGC 1 cut(s) 178
GluI GCNGC 1 cut(s) 844
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 2 cut(s) 109, 549
HapII CCGG 3 cut(s) 39, 63, 550
HgaI GACGC 1 cut(s) 385
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 6 cut(s) 139, 257, 269, 373, 488, 835
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 2 cut(s) 128, 464
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 3 cut(s) 39, 63, 550
HphI GGTGA 3 cut(s) 482, 754, 827
Hpy166II GTNNAC 1 cut(s) 217
Hpy188III TCNNGA 3 cut(s) 364, 776, 851
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 99, 732, 806
HpyCH4III ACNGT 1 cut(s) 429
HpyCH4V TGCA 4 cut(s) 232, 269, 599, 846
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 779, 886
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 6 cut(s) 139, 257, 269, 373, 488, 835
HspAI GCGC 1 cut(s) 177
KroI GCCGGC 1 cut(s) 549
KroNI GCCGGC 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 744
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 3 cut(s) 292, 360, 744
LmnI GCTCC 2 cut(s) 778, 909
Lsp1109I GCAGC 1 cut(s) 830
LweI GCATC 1 cut(s) 867
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 294, 362, 746
MboI GATC 3 cut(s) 292, 360, 744
MboII GAAGA 2 cut(s) 355, 391
MfeI CAATTG 1 cut(s) 194
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 285, 914
MluCI AATT 4 cut(s) 194, 385, 408, 718
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 7 cut(s) 118, 336, 339, 528, 582, 772, 899
Mph1103I ATGCAT 1 cut(s) 271
MroNI GCCGGC 1 cut(s) 549
MseI TTAA 3 cut(s) 216, 422, 899
MspA1I CMGCKG 1 cut(s) 843
MspI CCGG 3 cut(s) 39, 63, 550
MspR9I CCNGG 2 cut(s) 770, 924
MunI CAATTG 1 cut(s) 194
Mva1269I GAATGC 3 cut(s) 269, 308, 846
MvaI CCWGG 2 cut(s) 770, 924
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 3 cut(s) 33, 779, 886
NaeI GCCGGC 1 cut(s) 551
NdeII GATC 3 cut(s) 292, 360, 744
NgoMIV GCCGGC 1 cut(s) 549
NlaIII CATG 6 cut(s) 139, 257, 269, 373, 488, 835
NlaIV GGNNCC 5 cut(s) 37, 263, 492, 767, 774
NsbI TGCGCA 1 cut(s) 178
NsiI ATGCAT 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 3 cut(s) 269, 308, 846
PdiI GCCGGC 1 cut(s) 551
PfeI GAWTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 922
PkrI GCNGC 1 cut(s) 845
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 768, 922
PspGI CCWGG 2 cut(s) 768, 922
PspN4I GGNNCC 5 cut(s) 37, 263, 492, 767, 774
PspPI GGNCC 1 cut(s) 262
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 3 cut(s) 216, 422, 899
SatI GCNGC 1 cut(s) 844
Sau3AI GATC 3 cut(s) 292, 360, 744
Sau96I GGNCC 1 cut(s) 262
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 770, 924
SduI GDGCHC 2 cut(s) 285, 914
SetI ASST 8 cut(s) 175, 285, 418, 547, 651, 767, 796, 817
SfaNI GCATC 1 cut(s) 867
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 4 cut(s) 194, 385, 408, 718
SsiI CCGC 3 cut(s) 10, 349, 841
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 768, 922
TaaI ACNGT 1 cut(s) 429
TaqI TCGA 2 cut(s) 187, 363
TasI AATT 4 cut(s) 194, 385, 408, 718
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 3 cut(s) 216, 422, 899
Tru9I TTAA 3 cut(s) 216, 422, 899
TscAI CASTG 2 cut(s) 432, 763
TseI GCWGC 1 cut(s) 843
TspDTI ATGAA 4 cut(s) 17, 391, 551, 617
TspGWI ACGGA 1 cut(s) 601
TspRI CASTG 2 cut(s) 432, 763
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 1 cut(s) 408
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.