Rorug02G0635800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011661
Physical Location & Seq
Reverse (-)
74937506 .. 74940541
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0635800.1

Sequence Viewer

Length: 873 bp
ATGTGTCGAGGTGATACACAAGAATATTCAGATCTGAATAAGCTTGCAAGACGTTTTCTAAAAGGCAGCCAGAATTCTGTGGAAGGGGAAGCCAAAGATTTGCCTTCACGGGCTTATGTTCAGGAAATTGTTGAAGAAATTCGCAAGGGGGAACAGGGAGAGTGTCCCATATGTCTTGAAGCATTTGAAGATGCAGTATTGACACCTTGTGCTCATCGCTTATGCCGAGAATGTCTCTTGGCAAGTTGGCGGAATTCAACTTCTGGCTTATGTCCAGTTTGCAGGAAGAGTCTAAGTAAGCAAGATCTTATTACAGCACCAACTGACAGTCGGTTCCATGTTGATATTGAGAAAAACTGGGTGGAATCATCCAAAATAGTTATTCTTTTGTGTGAACTTGAATGTCTTCGTTCGTCAGGCTCAAAAAGCATTGTCTTCAGCCAGTGGACAGCCTTTCTGGACCTCCTGCAGATTCCTCTTTCTCGGAGTAACATTCCATTTTTGCGCCTGGATGGGACTCTGAATCAACAGCAGCGTGAAAAAGTGTTAAAACAGTTTTCAGAAGACAGTGACATTCTGGTGTTGCTAATGTCACTGAAGGCCGGAGGAGTTGGAATAAATTTAACAGCAGCATCCAATGCCTTTGTCTTGGATCCATGGTGGAATCCAGCTGTAGAGGAACAAGCGGTCATGCGTATTCATCGCATCGGACAAACAAAAAGTGTGATGATCAAGCGATTTATCGTGAAGGGAACAGTCGAGGAAAGAATGGAAGCAGTGCAAGCTCGTAAACAGCGGTTAATTTCTGGCGCCTTGACTGACCAAGAAGTTCGTTCGGCTCGCATAGAAGAACTAAAGATGCTTTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

290

Amino Acids

32.76

Weight (kDa)

7.49

Isoelectric Point (pI)

53.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 52 - 99 8e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 54 - 94 3.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 54 - 95 7.3e-09 Ring finger domain
zf-RING_5 PF14634 54 - 96 4e-06 zinc-RING finger domain
zf-C3HC4 PF00097 55 - 94 1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 55 - 92 6.3e-10 RING-type zinc-finger
Prok-RING_4 PF14447 55 - 102 9e-07 Prokaryotic RING finger family 4
zf-C3HC4_4 PF15227 55 - 94 2.4e-06 zinc finger of C3HC4-type, RING
RING_XB3-XBAT31 PF24921 55 - 98 6.4e-06 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Helicase_C PF00271 130 - 237 2.1e-21 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 809
AciI CCGC 3 cut(s) 250, 686, 796
AclWI GGATC 2 cut(s) 647, 660
AcsI RAATTY 4 cut(s) 73, 138, 253, 619
AcuI CTGAAG 2 cut(s) 421, 617
AcyI GRCGYC 1 cut(s) 810
AdeI CACNNNGTG 1 cut(s) 209
AgsI TTSAA 5 cut(s) 134, 179, 188, 258, 401
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 3 cut(s) 43, 671, 785
AluI AGCT 3 cut(s) 43, 671, 785
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 2 cut(s) 647, 660
AoxI GGCC 1 cut(s) 600
ApeKI GCWGC 3 cut(s) 66, 532, 629
ApoI RAATTY 4 cut(s) 73, 138, 253, 619
Asp700I GAANNNNTTC 2 cut(s) 138, 405
AspLEI GCGC 2 cut(s) 507, 812
AspS9I GGNCC 1 cut(s) 460
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 652
BanI GGYRCC 1 cut(s) 809
BbsI GAAGAC 3 cut(s) 398, 427, 570
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 3 cut(s) 78, 544, 641
BccI CCATC 1 cut(s) 506
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 1 cut(s) 729
BcoDI GTCTC 1 cut(s) 239
BfmI CTRYAG 2 cut(s) 467, 672
BfoI RGCGCY 1 cut(s) 813
BglII AGATCT 2 cut(s) 31, 304
BisI GCNGC 3 cut(s) 67, 533, 630
BlsI GCNGC 3 cut(s) 68, 534, 631
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 3 cut(s) 335, 654, 811
BmrFI CCNGG 1 cut(s) 509
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 4 cut(s) 181, 641, 714, 849
BmuI ACTGGG 1 cut(s) 367
BpiI GAAGAC 3 cut(s) 398, 427, 570
BplI GAGNNNNNCTC 2 cut(s) 219, 251
BsaHI GRCGYC 1 cut(s) 810
BsaJI CCNNGG 1 cut(s) 656
Bse1I ACTGG 3 cut(s) 275, 362, 442
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 656
BseGI GGATG 3 cut(s) 368, 517, 632
BseNI ACTGG 3 cut(s) 275, 362, 442
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 3 cut(s) 78, 544, 641
BshFI GGCC 1 cut(s) 602
BshNI GGYRCC 1 cut(s) 809
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 603
BslFI GGGAC 2 cut(s) 150, 529
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 2 cut(s) 150, 529
BsnI GGCC 1 cut(s) 602
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 4 cut(s) 31, 304, 652, 729
Bsp19I CCATGG 1 cut(s) 656
BspACI CCGC 3 cut(s) 250, 686, 796
BspANI GGCC 1 cut(s) 602
BspLI GGNNCC 3 cut(s) 335, 654, 811
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 2 cut(s) 647, 660
BspT107I GGYRCC 1 cut(s) 809
BsrI ACTGG 3 cut(s) 275, 362, 442
BssECI CCNNGG 1 cut(s) 656
BssMI GATC 4 cut(s) 31, 304, 652, 729
BssNI GRCGYC 1 cut(s) 810
BssT1I CCWWGG 1 cut(s) 656
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 4 cut(s) 329, 555, 569, 757
Bst6I CTCTTC 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 810
BstAPI GCANNNNNTGC 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 45, 783, 841
BstDEI CTNAG 1 cut(s) 293
BstDSI CCRYGG 1 cut(s) 656
BstF5I GGATG 3 cut(s) 368, 517, 632
BstH2I RGCGCY 1 cut(s) 813
BstHHI GCGC 2 cut(s) 507, 812
BstKTI GATC 4 cut(s) 34, 307, 655, 732
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 4 cut(s) 31, 304, 652, 729
BstMWI GCNNNNNNNGC 3 cut(s) 426, 638, 782
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 2 cut(s) 467, 672
BstV1I GCAGC 3 cut(s) 78, 544, 641
BstV2I GAAGAC 3 cut(s) 398, 427, 570
BstX2I RGATCY 3 cut(s) 31, 304, 652
BstYI RGATCY 3 cut(s) 31, 304, 652
BsuRI GGCC 1 cut(s) 602
BtgI CCRYGG 1 cut(s) 656
BtgZI GCGATG 2 cut(s) 200, 686
BtsCI GGATG 3 cut(s) 368, 517, 632
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 4 cut(s) 449, 574, 593, 783
Cac8I GCNNGC 3 cut(s) 45, 783, 841
CfoI GCGC 2 cut(s) 507, 812
Cfr13I GGNCC 1 cut(s) 460
CviAII CATG 3 cut(s) 338, 657, 691
DdeI CTNAG 1 cut(s) 293
DinI GGCGCC 1 cut(s) 811
DpnI GATC 4 cut(s) 33, 306, 654, 731
DpnII GATC 4 cut(s) 31, 304, 652, 729
DraIII CACNNNGTG 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
EciI GGCGGA 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 421, 617
EcoRI GAATTC 2 cut(s) 73, 253
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 656
EgeI GGCGCC 1 cut(s) 811
EheI GGCGCC 1 cut(s) 811
ErhI CCWWGG 1 cut(s) 656
FaeI CATG 3 cut(s) 341, 660, 694
FaiI YATR 9 cut(s) 117, 170, 172, 223, 271, 339, 658, 692, 845
FaqI GGGAC 2 cut(s) 150, 529
FatI CATG 3 cut(s) 337, 656, 690
FauNDI CATATG 1 cut(s) 170
FbaI TGATCA 1 cut(s) 729
Fnu4HI GCNGC 3 cut(s) 67, 533, 630
FokI GGATG 3 cut(s) 355, 524, 619
Fsp4HI GCNGC 3 cut(s) 67, 533, 630
GlaI GCGC 2 cut(s) 506, 811
GluI GCNGC 3 cut(s) 67, 533, 630
HaeII RGCGCY 1 cut(s) 813
HaeIII GGCC 1 cut(s) 602
HapII CCGG 1 cut(s) 603
HhaI GCGC 2 cut(s) 507, 812
Hin1I GRCGYC 1 cut(s) 810
Hin1II CATG 3 cut(s) 341, 660, 694
Hin6I GCGC 2 cut(s) 505, 810
HinP1I GCGC 2 cut(s) 505, 810
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 6 cut(s) 289, 365, 472, 517, 523, 664
HpaII CCGG 1 cut(s) 603
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 3 cut(s) 395, 447, 791
Hpy188I TCNGA 6 cut(s) 31, 36, 486, 522, 562, 710
Hpy188III TCNNGA 4 cut(s) 122, 176, 458, 745
Hpy8I GTNNAC 3 cut(s) 395, 447, 791
HpyAV CCTTC 4 cut(s) 77, 114, 592, 742
HpyCH4III ACNGT 4 cut(s) 329, 555, 569, 757
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 5 cut(s) 47, 194, 282, 469, 781
HpyF10VI GCNNNNNNNGC 3 cut(s) 426, 638, 782
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 52
Hsp92I GRCGYC 1 cut(s) 810
Hsp92II CATG 3 cut(s) 341, 660, 694
HspAI GCGC 2 cut(s) 505, 810
KasI GGCGCC 1 cut(s) 809
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 4 cut(s) 31, 304, 652, 729
Lsp1109I GCAGC 3 cut(s) 78, 544, 641
LweI GCATC 4 cut(s) 181, 641, 714, 849
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 3 cut(s) 488, 569, 591
MalI GATC 4 cut(s) 33, 306, 654, 731
MboI GATC 4 cut(s) 31, 304, 652, 729
MboII GAAGA 7 cut(s) 146, 200, 298, 398, 427, 575, 860
MflI RGATCY 3 cut(s) 31, 304, 652
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 6 cut(s) 73, 126, 138, 253, 619, 801
Mly113I GGCGCC 1 cut(s) 810
MlyI GAGTC 2 cut(s) 298, 511
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 5 cut(s) 473, 486, 599, 670, 754
MroXI GAANNNNTTC 2 cut(s) 138, 405
MseI TTAA 3 cut(s) 548, 623, 800
MslI CAYNNNNRTG 1 cut(s) 578
MspA1I CMGCKG 2 cut(s) 671, 796
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 426, 638, 782
NarI GGCGCC 1 cut(s) 810
NcoI CCATGG 1 cut(s) 656
NdeI CATATG 1 cut(s) 170
NdeII GATC 4 cut(s) 31, 304, 652, 729
NlaIII CATG 3 cut(s) 341, 660, 694
NlaIV GGNNCC 3 cut(s) 335, 654, 811
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 2 cut(s) 569, 591
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PdmI GAANNNNTTC 2 cut(s) 138, 405
PfeI GAWTC 4 cut(s) 365, 472, 523, 664
PkrI GCNGC 3 cut(s) 68, 534, 631
PleI GAGTC 2 cut(s) 297, 511
PluTI GGCGCC 1 cut(s) 813
PpsI GAGTC 2 cut(s) 297, 511
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 3 cut(s) 335, 654, 811
PspPI GGNCC 1 cut(s) 460
PstI CTGCAG 1 cut(s) 471
PsuI RGATCY 3 cut(s) 31, 304, 652
PvuII CAGCTG 1 cut(s) 671
RseI CAYNNNNRTG 1 cut(s) 578
SaqAI TTAA 3 cut(s) 548, 623, 800
SatI GCNGC 3 cut(s) 67, 533, 630
Sau3AI GATC 4 cut(s) 31, 304, 652, 729
Sau96I GGNCC 1 cut(s) 460
SchI GAGTC 2 cut(s) 298, 511
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 214
SetI ASST 7 cut(s) 13, 45, 55, 208, 465, 673, 787
SfaNI GCATC 4 cut(s) 181, 641, 714, 849
SfcI CTRYAG 2 cut(s) 467, 672
SfoI GGCGCC 1 cut(s) 811
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 578
Sse9I AATT 6 cut(s) 73, 126, 138, 253, 619, 801
SsiI CCGC 3 cut(s) 250, 686, 796
SspDI GGCGCC 1 cut(s) 809
SspI AATATT 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 656
TaaI ACNGT 4 cut(s) 329, 555, 569, 757
TaiI ACGT 1 cut(s) 55
TaqI TCGA 2 cut(s) 7, 759
TasI AATT 6 cut(s) 73, 126, 138, 253, 619, 801
TfiI GAWTC 4 cut(s) 365, 472, 523, 664
Tru1I TTAA 3 cut(s) 548, 623, 800
Tru9I TTAA 3 cut(s) 548, 623, 800
TscAI CASTG 4 cut(s) 449, 574, 600, 783
TseFI GTSAC 2 cut(s) 569, 591
TseI GCWGC 3 cut(s) 66, 532, 629
Tsp45I GTSAC 2 cut(s) 569, 591
TspDTI ATGAA 1 cut(s) 689
TspRI CASTG 4 cut(s) 449, 574, 600, 783
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 4 cut(s) 73, 138, 253, 619
XmnI GAANNNNTTC 2 cut(s) 138, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.