Rorug02G0635900

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011661
Physical Location & Seq
Reverse (-)
74952490 .. 74955647
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0635900.1

Sequence Viewer

Length: 1638 bp
ATGGCTTTGAATCTCTCTCGTGGCTCAGTTCTGCCTAAGTATCCTTCCCCAGAGCATAGTTTGTGTTCTTCTGTTAGGTGTTCTAATGGGTATGTGAGGGATCAATGTAAAGAGAAGAGTTTGGATGGTGATGCATCGAGTTCGAATTCGGAGTTGAAGAAGAATACCCATTACAAGGGTGAAGTGGGTGGCTATTGTGAGCCTTCTGTTGATGATATTCTTGATAGGTTGCCTGTTGATCCTTTTGGGATGGATATGAGGTCTACTTTTACGGCGATTAGTGGTTGGCTTGAGGATTTTGAGAAGGGGTGTGAGTCTTATGGGTTTGGGGTGGATGAGCAGAAGAAGAAGGTGGTGGATGGTGGGTTGTTTGCTGGTCTGAATTGGGTTTGGAATAGCGCTGTGATATTTCAGCCGGAACTGGGTGAGGTGAAGTTTGATGGAATATCGATTCCATATGATAGCTTTGATGGGTTTGGGATCAATGATGGAGGTTTTGTATTGGACGACAATGTGGAAGATTTTCTGGGTTTTGGCCATGTGGTGAACTTGGATGTTAGTTATGGAGCTAAGGAACTGCCGGTTGGAACTATGGAAAGGCCAGAATGTAGCATAAAGAATTTTGATAGTGAAGGAGGTGCTCCACATGATGCTATGTTTTATGCATTGGGTTATCTCGGCGTTCAGGATCTTCTTTCAGTTCAACGGGTTTGCAGGTCTTTGAATCATGCAGTCAAAAGTGATCCTCTTTTATGGAGGAGCATTAACATTGATTGGCCGTTGAATGAGAGGATCACCGATGATGCTCTTGTAAATTTAACTAGCAGGGCTCAGGGTAATCTTCAAACCTTAAGTCTAGTGCATTGTGCATGGATTACCGATATTGGGCTGCATCGCGTGTTTGACAGCAATCCAAGGCTGACAAAATTAAGTATTCCAGGATGTATAAAAATAAGTGTTGAGAGCATCTTGTTAAACCTAAGGACCTTGAAGTCTGCAGGCAAACCTGGAATAAAGCAGTTAAGAATTGGAGGTCTCTCTGGTATAACAGACAAGCAGTTCGAAGAGTTAAAATTCCTGCTTGGTGCAGATAACCATATCCAGGTTAAAGCCCCAAAACCACGGTATTTTCATGGTGGGCTATCATATCTCTCTTGTGATGATGATTGCGCCATCGACATTGAAGCATGCCCCAGGTGCCAGAGACTAAGTTTAGTTTATGATTGCCCAGCAGAGAGTTGCCAAGGGAAATATCAGAAGGCTCAGATGTGCAGGGCTTGCATACTCTGCATAACTCGCTGTATTAGCTGTGGGTGTTGCCTGGGAGACTGCGATTATGTGGAGACGTTTTGCCTACAGTTGCTTTGTTTGGAATGTTTGGAGAACCTACTCAACTGTCAAGAGAGGCATGGAGAAAAAGGTGCTACCAAATGTGCCATATTTTGTCAGGAGAGCAGCTCATTCCCAAGTGATTCCCGTCCTGAGGGGTTGCCAAAGGCAATGAAGAAAACTATCTGGGAAACTTTACGGAGTGGAATTGAAGCATGGAAGCGATATTCGTTGTCATCATCATCATCATCGAAGTTGAGCAGCAGGCAGAAGGTTCTGGGAGAAGCAGTTGGACTGTTGAAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

545

Amino Acids

60.08

Weight (kDa)

5.46

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 215 - 257 8.5e-08 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 991
Acc36I ACCTGC 1 cut(s) 705
AccB1I GGYRCC 1 cut(s) 1197
AccI GTMKAC 1 cut(s) 263
AccII CGCG 1 cut(s) 897
AclWI GGATC 6 cut(s) 108, 233, 488, 696, 737, 800
AcoI YGGCCR 2 cut(s) 535, 776
AcsI RAATTY 4 cut(s) 145, 619, 814, 1073
AfeI AGCGCT 1 cut(s) 400
AfiI CCNNNNNNNGG 5 cut(s) 175, 422, 885, 1102, 1483
AflII CTTAAG 1 cut(s) 850
AjnI CCWGG 5 cut(s) 937, 1006, 1101, 1193, 1320
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AluBI AGCT 4 cut(s) 465, 569, 1308, 1458
AluI AGCT 4 cut(s) 465, 569, 1308, 1458
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 4 cut(s) 1040, 1198, 1320, 1337
AlwI GGATC 6 cut(s) 108, 233, 488, 696, 737, 800
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 3 cut(s) 535, 599, 776
ApeKI GCWGC 3 cut(s) 889, 1455, 1590
ApoI RAATTY 4 cut(s) 145, 619, 814, 1073
ArsI GACNNNNNNTTYG 4 cut(s) 838, 870, 1043, 1075
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 2 cut(s) 401, 1172
AspS9I GGNCC 1 cut(s) 984
AsuHPI GGTGA 6 cut(s) 140, 191, 437, 442, 556, 787
AsuII TTCGAA 2 cut(s) 143, 1062
AvaII GGWCC 1 cut(s) 984
AxyI CCTNAGG 2 cut(s) 980, 1482
BalI TGGCCA 1 cut(s) 537
BanI GGYRCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 832
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 3 cut(s) 876, 1467, 1602
BccI CCATC 7 cut(s) 119, 244, 353, 434, 464, 482, 1181
BceAI ACGGC 2 cut(s) 288, 763
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BciT130I CCWGG 5 cut(s) 939, 1008, 1103, 1195, 1322
BciVI GTATCC 1 cut(s) 51
BcoDI GTCTC 4 cut(s) 1040, 1198, 1320, 1337
BfaI CTAG 2 cut(s) 822, 857
BfmI CTRYAG 2 cut(s) 996, 1355
BfoI RGCGCY 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 850
BfuAI ACCTGC 1 cut(s) 705
BfuI GTATCC 1 cut(s) 51
BisI GCNGC 3 cut(s) 890, 1456, 1591
BlsI GCNGC 3 cut(s) 891, 1457, 1592
Bme1390I CCNGG 5 cut(s) 939, 1008, 1103, 1195, 1322
Bme18I GGWCC 1 cut(s) 984
BmgT120I GGNCC 1 cut(s) 984
BmiI GGNNCC 1 cut(s) 1199
BmrFI CCNGG 5 cut(s) 939, 1008, 1103, 1195, 1322
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 6 cut(s) 121, 143, 640, 793, 901, 975
BmuI ACTGGG 1 cut(s) 431
BplI GAGNNNNNCTC 2 cut(s) 1442, 1474
Bpu10I CCTNAGC 2 cut(s) 570, 831
Bpu14I TTCGAA 2 cut(s) 143, 1062
BpuEI CTTGAG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 449
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 5 cut(s) 914, 1121, 1193, 1243, 1321
BsaXI ACNNNNNCTCC 4 cut(s) 483, 513, 1602, 1632
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 422, 885, 1102, 1483
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 1 cut(s) 426
Bse21I CCTNAGG 2 cut(s) 980, 1482
Bse3DI GCAATG 1 cut(s) 1506
BseBI CCWGG 5 cut(s) 939, 1008, 1103, 1195, 1322
BseCI ATCGAT 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 914, 1121, 1193, 1243, 1321
BseGI GGATG 6 cut(s) 130, 255, 340, 364, 559, 947
BseLI CCNNNNNNNGG 5 cut(s) 175, 422, 885, 1102, 1483
BseMI GCAATG 1 cut(s) 1506
BseMII CTCAG 4 cut(s) 39, 845, 1277, 1473
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 1 cut(s) 772
BseXI GCAGC 3 cut(s) 876, 1467, 1602
BseYI CCCAGC 1 cut(s) 1228
BsgI GTGCAG 2 cut(s) 1107, 1291
Bsh1236I CGCG 1 cut(s) 897
BshFI GGCC 3 cut(s) 537, 601, 778
BshNI GGYRCC 1 cut(s) 1197
BshVI ATCGAT 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 2 cut(s) 416, 581
BslI CCNNNNNNNGG 5 cut(s) 175, 422, 885, 1102, 1483
BsmAI GTCTC 4 cut(s) 1040, 1198, 1320, 1337
BsmBI CGTCTC 1 cut(s) 1337
BsnI GGCC 3 cut(s) 537, 601, 778
Bso31I GGTCTC 1 cut(s) 1040
Bsp119I TTCGAA 2 cut(s) 143, 1062
Bsp1286I GDGCHC 2 cut(s) 643, 832
Bsp143I GATC 6 cut(s) 100, 238, 480, 688, 742, 792
BspANI GGCC 3 cut(s) 537, 601, 778
BspCNI CTCAG 4 cut(s) 38, 844, 1276, 1474
BspDI ATCGAT 1 cut(s) 449
BspFNI CGCG 1 cut(s) 897
BspLI GGNNCC 1 cut(s) 1199
BspMAI CTGCAG 1 cut(s) 1000
BspMI ACCTGC 1 cut(s) 705
BspPI GGATC 6 cut(s) 108, 233, 488, 696, 737, 800
BspT104I TTCGAA 2 cut(s) 143, 1062
BspT107I GGYRCC 1 cut(s) 1197
BspTI CTTAAG 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 1506
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 5 cut(s) 914, 1121, 1193, 1243, 1321
BssMI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 914, 1243
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 5 cut(s) 939, 1008, 1103, 1195, 1322
Bst4CI ACNGT 4 cut(s) 1125, 1359, 1397, 1626
Bst6I CTCTTC 2 cut(s) 110, 1059
BstAFI CTTAAG 1 cut(s) 850
BstAPI GCANNNNNTGC 2 cut(s) 1278, 1287
BstBI TTCGAA 2 cut(s) 143, 1062
BstC8I GCNNGC 4 cut(s) 1000, 1189, 1279, 1595
BstDEI CTNAG 8 cut(s) 25, 36, 570, 831, 980, 1208, 1263, 1482
BstDSI CCRYGG 1 cut(s) 1121
BstF5I GGATG 6 cut(s) 130, 255, 340, 364, 559, 947
BstFNI CGCG 1 cut(s) 897
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 2 cut(s) 401, 1172
BstKTI GATC 6 cut(s) 103, 241, 483, 691, 745, 795
BstMAI GTCTC 4 cut(s) 1040, 1198, 1320, 1337
BstMBI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
BstMWI GCNNNNNNNGC 5 cut(s) 1197, 1278, 1287, 1296, 1305
BstNI CCWGG 5 cut(s) 939, 1008, 1103, 1195, 1322
BstNSI RCATGY 1 cut(s) 1191
BstSCI CCNGG 5 cut(s) 937, 1006, 1101, 1193, 1320
BstSFI CTRYAG 2 cut(s) 996, 1355
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 3 cut(s) 876, 1467, 1602
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 449
Bsu36I CCTNAGG 2 cut(s) 980, 1482
BsuI GTATCC 1 cut(s) 51
BsuRI GGCC 3 cut(s) 537, 601, 778
BsuTUI ATCGAT 1 cut(s) 449
BtgI CCRYGG 1 cut(s) 1121
BtgZI GCGATG 1 cut(s) 878
BtsCI GGATG 6 cut(s) 130, 255, 340, 364, 559, 947
BveI ACCTGC 1 cut(s) 705
Cac8I GCNNGC 4 cut(s) 1000, 1189, 1279, 1595
CfoI GCGC 2 cut(s) 401, 1172
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 984
ClaI ATCGAT 1 cut(s) 449
CviAII CATG 8 cut(s) 539, 647, 728, 870, 1133, 1188, 1409, 1545
DdeI CTNAG 8 cut(s) 25, 36, 570, 831, 980, 1208, 1263, 1482
DpnI GATC 6 cut(s) 102, 240, 482, 690, 744, 794
DpnII GATC 6 cut(s) 100, 238, 480, 688, 742, 792
DrdI GACNNNNNNGTC 1 cut(s) 991
DseDI GACNNNNNNGTC 1 cut(s) 991
EaeI YGGCCR 2 cut(s) 535, 776
Eam1104I CTCTTC 2 cut(s) 110, 1059
EarI CTCTTC 2 cut(s) 110, 1059
Eco130I CCWWGG 2 cut(s) 914, 1243
Eco24I GRGCYC 1 cut(s) 832
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 1 cut(s) 984
Eco47III AGCGCT 1 cut(s) 400
Eco81I CCTNAGG 2 cut(s) 980, 1482
EcoO109I RGGNCCY 1 cut(s) 984
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 5 cut(s) 937, 1006, 1101, 1193, 1320
EcoT14I CCWWGG 2 cut(s) 914, 1243
EcoT22I ATGCAT 2 cut(s) 136, 667
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 2 cut(s) 914, 1243
Esp3I CGTCTC 1 cut(s) 1337
FaeI CATG 8 cut(s) 542, 650, 731, 873, 1136, 1191, 1412, 1548
FatI CATG 8 cut(s) 538, 646, 727, 869, 1132, 1187, 1408, 1544
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 3 cut(s) 890, 1456, 1591
FokI GGATG 6 cut(s) 137, 262, 347, 371, 566, 954
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 3 cut(s) 890, 1456, 1591
FspBI CTAG 2 cut(s) 822, 857
GlaI GCGC 2 cut(s) 400, 1171
GluI GCNGC 3 cut(s) 890, 1456, 1591
GsaI CCCAGC 1 cut(s) 1232
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 3 cut(s) 537, 601, 778
HapII CCGG 2 cut(s) 416, 581
HhaI GCGC 2 cut(s) 401, 1172
Hin1II CATG 8 cut(s) 542, 650, 731, 873, 1136, 1191, 1412, 1548
Hin6I GCGC 2 cut(s) 399, 1170
HinP1I GCGC 2 cut(s) 399, 1170
HinfI GANTC 5 cut(s) 10, 314, 451, 724, 1472
HpaII CCGG 2 cut(s) 416, 581
HphI GGTGA 6 cut(s) 140, 191, 437, 442, 556, 787
Hpy166II GTNNAC 2 cut(s) 264, 547
Hpy188I TCNGA 4 cut(s) 151, 381, 1257, 1266
Hpy188III TCNNGA 5 cut(s) 221, 686, 1400, 1448, 1481
Hpy8I GTNNAC 2 cut(s) 264, 547
HpyAV CCTTC 8 cut(s) 54, 213, 298, 343, 626, 1252, 1594, 1624
HpyCH4III ACNGT 4 cut(s) 1125, 1359, 1397, 1626
HpyCH4IV ACGT 1 cut(s) 1346
HpyF10VI GCNNNNNNNGC 5 cut(s) 1197, 1278, 1287, 1296, 1305
HpyF3I CTNAG 8 cut(s) 25, 36, 570, 831, 980, 1208, 1263, 1482
HpySE526I ACGT 1 cut(s) 1346
Hsp92II CATG 8 cut(s) 542, 650, 731, 873, 1136, 1191, 1412, 1548
HspAI GCGC 2 cut(s) 399, 1170
Kzo9I GATC 6 cut(s) 100, 238, 480, 688, 742, 792
LmnI GCTCC 3 cut(s) 566, 646, 759
Lsp1109I GCAGC 3 cut(s) 876, 1467, 1602
LweI GCATC 6 cut(s) 121, 143, 640, 793, 901, 975
MaeI CTAG 2 cut(s) 822, 857
MaeII ACGT 1 cut(s) 1346
MalI GATC 6 cut(s) 102, 240, 482, 690, 744, 794
MboI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 2 cut(s) 643, 832
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 8 cut(s) 145, 382, 619, 814, 926, 1026, 1073, 1536
MluNI TGGCCA 1 cut(s) 537
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 565, 1600
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 2 cut(s) 136, 667
MroXI GAANNNNTTC 1 cut(s) 522
MscI TGGCCA 1 cut(s) 537
MseI TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
Msp20I TGGCCA 1 cut(s) 537
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 2 cut(s) 416, 581
MspR9I CCNGG 5 cut(s) 939, 1008, 1103, 1195, 1322
MvaI CCWGG 5 cut(s) 939, 1008, 1103, 1195, 1322
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 5 cut(s) 1197, 1278, 1287, 1296, 1305
NdeI CATATG 1 cut(s) 457
NdeII GATC 6 cut(s) 100, 238, 480, 688, 742, 792
NlaIII CATG 8 cut(s) 542, 650, 731, 873, 1136, 1191, 1412, 1548
NlaIV GGNNCC 1 cut(s) 1199
NmeAIII GCCGAG 1 cut(s) 657
NsiI ATGCAT 2 cut(s) 136, 667
NspI RCATGY 1 cut(s) 1191
NspV TTCGAA 2 cut(s) 143, 1062
PaeI GCATGC 1 cut(s) 1191
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 4 cut(s) 10, 451, 724, 1472
PfoI TCCNGGA 1 cut(s) 937
PkrI GCNGC 3 cut(s) 891, 1457, 1592
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 984
Psp6I CCWGG 5 cut(s) 937, 1006, 1101, 1193, 1320
PspFI CCCAGC 1 cut(s) 1228
PspGI CCWGG 5 cut(s) 937, 1006, 1101, 1193, 1320
PspN4I GGNNCC 1 cut(s) 1199
PspPI GGNCC 1 cut(s) 984
PspPPI RGGWCCY 1 cut(s) 984
PstI CTGCAG 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 688
SaqAI TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
SatI GCNGC 3 cut(s) 890, 1456, 1591
Sau3AI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
Sau96I GGNCC 1 cut(s) 984
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 5 cut(s) 939, 1008, 1103, 1195, 1322
SduI GDGCHC 2 cut(s) 643, 832
SfaNI GCATC 6 cut(s) 121, 143, 640, 793, 901, 975
SfcI CTRYAG 2 cut(s) 996, 1355
SfuI TTCGAA 2 cut(s) 143, 1062
SinI GGWCC 1 cut(s) 984
SmlI CTYRAG 2 cut(s) 290, 850
SmoI CTYRAG 2 cut(s) 290, 850
SphI GCATGC 1 cut(s) 1191
Sse9I AATT 8 cut(s) 145, 382, 619, 814, 926, 1026, 1073, 1536
SspMI CTAG 2 cut(s) 822, 857
StyD4I CCNGG 5 cut(s) 937, 1006, 1101, 1193, 1320
StyI CCWWGG 2 cut(s) 914, 1243
TaaI ACNGT 4 cut(s) 1125, 1359, 1397, 1626
TaiI ACGT 1 cut(s) 1349
TaqI TCGA 6 cut(s) 137, 143, 449, 1062, 1176, 1580
TasI AATT 8 cut(s) 145, 382, 619, 814, 926, 1026, 1073, 1536
TfiI GAWTC 4 cut(s) 10, 451, 724, 1472
Tru1I TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
Tru9I TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
TseI GCWGC 3 cut(s) 889, 1455, 1590
TspDTI ATGAA 2 cut(s) 1121, 1517
TspGWI ACGGA 1 cut(s) 1543
Vha464I CTTAAG 1 cut(s) 850
VpaK11BI GGWCC 1 cut(s) 984
XapI RAATTY 4 cut(s) 145, 619, 814, 1073
XceI RCATGY 1 cut(s) 1191
XmiI GTMKAC 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 2 cut(s) 822, 857
Zsp2I ATGCAT 2 cut(s) 136, 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.