Rorug03G0096700

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017327
Physical Location & Seq
Forward (+)
7544681 .. 7546053
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0096700.1

Sequence Viewer

Length: 987 bp
ATGGAGTGTAGTAGTTGGCCAGTTGAAAGCGTGTGTTTGTCGAATAGGCAGAGGGTACTGAAGGAAGAGCTGGTTCTAGGGCATCAACTTGCGAATCAACTTGGCAGGGTTGTTTCTAATGGGGATTTTAGATCGGCCGAAGGTCTTGTTACGAAGATTCTGAGCTCGTTCAGTAATAGCCTTTCCATCTTAAATGGAAATCATGAGTCTGAGGAGGAGGAGGAGGAGCTTAATATTTCTCCTATTCGAGCCAATAATATTACTATTGCTCCTCCTCCTTCTATTCTAGAATTGTCATCTTGGGATGCTCATAATGCTGTTGAGAGTGCTGCGTCCATGGATCTCAGAGGTTCTTTCAAGAGAAGAAAGAAGATGACTTCACACGCATGGTCCAGAGACAGTCCAGATTTCAATGACGACGGTCATGCATGGAGAAAGTACGGACAGAAACAGATCCAGAATGCTATCCACCCAAGGAGCTACTTTAGGTGCAGTCATAAGTACGATCAAGGCTGCACAGCAACCAAGCAGGTGCAAAAACTCGACCATTATCCACAGCTATTTCGGACTACATATTATGGAAATCACACATGCACTAAAAGCTTCCTCAAAGCTCCTGATTTTTTCTTGGATACTACAAATGCTACTCCTACAGAAGTGTCTAATTCCAAGTTTATGATCATATATAACTCAAACAACAGCCTCACAAAAATTAAACAAGAACAGCCTTTGTTCTCATCAACCAAGAACGAGCTCATCAAGTCTAACCATACGACTGGCAACAAGTTATCATCTAGTGATTATCTTGTGTCATCTGGTACGGGGATGGTCGGGCCCTCTGAACCCATCAGCGGACTGCCATCACAGATTGAGCTTGATCGTAAAGGTATGATTGATCTTGGGCAGAGATGGGACGCTTATTATTTGATGAAGAAATTTTGCCTTATGAACTTTGGTGATGAATGCAAGCGTACTTGGCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

328

Amino Acids

37.12

Weight (kDa)

8.09

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 139 - 198 9.8e-23 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017327)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g15370 FvH4_6g15371
rosa_chinensis RchiOBHm_Chr3g0468811 RchiOBHm_Chr3g0468821
rosa_laevigata RLG00000024407
rosa_multiflora Rmu_sc0001639.1_g000023
rosa_roxburghii Rroxscaffold_6G00412530
rosa_rugosa Rorug03G0096700
rosa_samantha Rh3AG146400 Rh3AG146500 Rh3BG169000
rosa_wichuraiana Rw3G013770 Rw3G013780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 520
AciI CCGC 1 cut(s) 852
AclWI GGATC 2 cut(s) 348, 448
AcoI YGGCCR 2 cut(s) 17, 135
AcsI RAATTY 1 cut(s) 935
AcuI CTGAAG 1 cut(s) 80
AfaI GTAC 5 cut(s) 57, 440, 503, 820, 973
AfiI CCNNNNNNNGG 1 cut(s) 851
AgsI TTSAA 3 cut(s) 26, 358, 412
AluBI AGCT 9 cut(s) 70, 165, 229, 480, 559, 603, 614, 754, 874
AluI AGCT 9 cut(s) 70, 165, 229, 480, 559, 603, 614, 754, 874
Alw21I GWGCWC 2 cut(s) 167, 756
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 2 cut(s) 348, 448
AoxI GGCC 3 cut(s) 17, 135, 833
ApaI GGGCCC 1 cut(s) 837
ApeKI GCWGC 2 cut(s) 329, 513
ApoI RAATTY 1 cut(s) 935
AspS9I GGNCC 3 cut(s) 390, 833, 834
AsuHPI GGTGA 1 cut(s) 968
AvaII GGWCC 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 837
BalI TGGCCA 1 cut(s) 19
BanII GRGCYC 3 cut(s) 167, 756, 837
Bbv12I GWGCWC 2 cut(s) 167, 756
BbvI GCAGC 2 cut(s) 316, 500
BccI CCATC 5 cut(s) 194, 820, 854, 868, 903
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciVI GTATCC 1 cut(s) 625
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 1 cut(s) 390
BfaI CTAG 3 cut(s) 77, 287, 795
BfmI CTRYAG 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 1 cut(s) 625
BisI GCNGC 2 cut(s) 330, 514
BlsI GCNGC 2 cut(s) 331, 515
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 3 cut(s) 390, 833, 834
BmiI GGNNCC 1 cut(s) 835
BmsI GCATC 2 cut(s) 91, 295
BoxI GACNNNNGTC 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 336, 473
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 851
Bse1I ACTGG 2 cut(s) 20, 781
BseDI CCNNGG 2 cut(s) 336, 473
BseGI GGATG 2 cut(s) 310, 831
BseLI CCNNNNNNNGG 1 cut(s) 851
BseMII CTCAG 3 cut(s) 152, 201, 358
BseNI ACTGG 2 cut(s) 20, 781
BseRI GAGGAG 7 cut(s) 227, 230, 233, 236, 239, 261, 264
BseSI GKGCMC 1 cut(s) 837
BseX3I CGGCCG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 316, 500
BsgI GTGCAG 2 cut(s) 499, 511
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 19, 137, 835
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 167, 756
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 1 cut(s) 851
BsmAI GTCTC 1 cut(s) 390
BsmFI GGGAC 1 cut(s) 926
BsmI GAATGC 2 cut(s) 466, 968
BsnI GGCC 3 cut(s) 19, 137, 835
Bsp120I GGGCCC 1 cut(s) 833
Bsp1286I GDGCHC 3 cut(s) 167, 756, 837
Bsp143I GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 1 cut(s) 852
BspANI GGCC 3 cut(s) 19, 137, 835
BspCNI CTCAG 3 cut(s) 153, 202, 357
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 835
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 2 cut(s) 348, 448
BspQI GCTCTTC 1 cut(s) 60
BsrI ACTGG 2 cut(s) 20, 781
BssECI CCNNGG 2 cut(s) 336, 473
BssMI GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
BssT1I CCWWGG 2 cut(s) 336, 473
Bst4CI ACNGT 2 cut(s) 401, 422
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 968
BstDEI CTNAG 3 cut(s) 161, 210, 344
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 310, 831
BstKTI GATC 7 cut(s) 134, 343, 456, 508, 681, 880, 898
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 3 cut(s) 314, 600, 976
BstNSI RCATGY 1 cut(s) 594
BstPAI GACNNNNGTC 1 cut(s) 420
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 837
BstV1I GCAGC 2 cut(s) 316, 500
BstX2I RGATCY 2 cut(s) 340, 453
BstXI CCANNNNNNTGG 1 cut(s) 776
BstYI RGATCY 2 cut(s) 340, 453
BstZI CGGCCG 1 cut(s) 135
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 3 cut(s) 19, 137, 835
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 2 cut(s) 310, 831
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 1 cut(s) 968
CciI TCATGA 1 cut(s) 202
Cfr13I GGNCC 3 cut(s) 390, 833, 834
CseI GACGC 2 cut(s) 321, 923
Csp6I GTAC 5 cut(s) 56, 439, 502, 819, 972
CviAII CATG 6 cut(s) 203, 337, 387, 425, 429, 591
CviQI GTAC 5 cut(s) 56, 439, 502, 819, 972
DdeI CTNAG 3 cut(s) 161, 210, 344
DpnI GATC 7 cut(s) 133, 342, 455, 507, 680, 879, 897
DpnII GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
EaeI YGGCCR 2 cut(s) 17, 135
EagI CGGCCG 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Ecl136II GAGCTC 2 cut(s) 165, 754
EclXI CGGCCG 1 cut(s) 135
Eco130I CCWWGG 2 cut(s) 336, 473
Eco24I GRGCYC 3 cut(s) 167, 756, 837
Eco47I GGWCC 1 cut(s) 390
Eco52I CGGCCG 1 cut(s) 135
Eco53kI GAGCTC 2 cut(s) 165, 754
Eco57I CTGAAG 1 cut(s) 80
EcoICRI GAGCTC 2 cut(s) 165, 754
EcoO109I RGGNCCY 1 cut(s) 834
EcoT14I CCWWGG 2 cut(s) 336, 473
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 3 cut(s) 167, 756, 837
ErhI CCWWGG 2 cut(s) 336, 473
FaeI CATG 6 cut(s) 206, 340, 390, 428, 432, 594
FaqI GGGAC 1 cut(s) 926
FatI CATG 6 cut(s) 202, 336, 386, 424, 428, 590
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 2 cut(s) 330, 514
FokI GGATG 2 cut(s) 317, 838
FriOI GRGCYC 3 cut(s) 167, 756, 837
Fsp4HI GCNGC 2 cut(s) 330, 514
FspBI CTAG 3 cut(s) 77, 287, 795
GluI GCNGC 2 cut(s) 330, 514
HaeIII GGCC 3 cut(s) 19, 137, 835
HgaI GACGC 2 cut(s) 321, 923
Hin1II CATG 6 cut(s) 206, 340, 390, 428, 432, 594
HindIII AAGCTT 1 cut(s) 601
HinfI GANTC 3 cut(s) 94, 157, 206
HphI GGTGA 1 cut(s) 968
Hpy188I TCNGA 5 cut(s) 162, 211, 347, 567, 841
Hpy188III TCNNGA 7 cut(s) 203, 287, 358, 393, 404, 457, 617
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 3 cut(s) 55, 134, 288
HpyCH4III ACNGT 2 cut(s) 401, 422
HpyCH4V TGCA 6 cut(s) 428, 492, 516, 535, 594, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 600, 976
HpyF3I CTNAG 3 cut(s) 161, 210, 344
Hsp92II CATG 6 cut(s) 206, 340, 390, 428, 432, 594
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 4 cut(s) 226, 274, 477, 619
Lsp1109I GCAGC 2 cut(s) 316, 500
LweI GCATC 2 cut(s) 91, 295
MaeI CTAG 3 cut(s) 77, 287, 795
MaeIII GTNAC 1 cut(s) 148
MalI GATC 7 cut(s) 133, 342, 455, 507, 680, 879, 897
MboI GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
MboII GAAGA 5 cut(s) 77, 166, 375, 382, 943
MflI RGATCY 2 cut(s) 340, 453
MhlI GDGCHC 3 cut(s) 167, 756, 837
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 4 cut(s) 290, 664, 711, 935
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 1 cut(s) 215
Mox20I TGGCCA 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 430
MscI TGGCCA 1 cut(s) 19
MseI TTAA 3 cut(s) 191, 231, 714
MslI CAYNNNNRTG 1 cut(s) 385
Msp20I TGGCCA 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 852
Mva1269I GAATGC 2 cut(s) 466, 968
MwoI GCNNNNNNNGC 3 cut(s) 314, 600, 976
NcoI CCATGG 1 cut(s) 336
NdeII GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
NlaIII CATG 6 cut(s) 206, 340, 390, 428, 432, 594
NlaIV GGNNCC 1 cut(s) 835
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 594
PagI TCATGA 1 cut(s) 202
PaqCI CACCTGC 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 60
PctI GAATGC 2 cut(s) 466, 968
PfeI GAWTC 2 cut(s) 94, 157
PkrI GCNGC 2 cut(s) 331, 515
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 420
Psp124BI GAGCTC 2 cut(s) 167, 756
PspN4I GGNNCC 1 cut(s) 835
PspOMI GGGCCC 1 cut(s) 833
PspPI GGNCC 3 cut(s) 390, 833, 834
PsuI RGATCY 2 cut(s) 340, 453
RsaI GTAC 5 cut(s) 57, 440, 503, 820, 973
RsaNI GTAC 5 cut(s) 56, 439, 502, 819, 972
RseI CAYNNNNRTG 1 cut(s) 385
SacI GAGCTC 2 cut(s) 167, 756
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 3 cut(s) 191, 231, 714
SatI GCNGC 2 cut(s) 330, 514
Sau3AI GATC 7 cut(s) 131, 340, 453, 505, 678, 877, 895
Sau96I GGNCC 3 cut(s) 390, 833, 834
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 3 cut(s) 167, 756, 837
SfaNI GCATC 2 cut(s) 91, 295
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 1 cut(s) 390
SmiMI CAYNNNNRTG 1 cut(s) 385
Sse9I AATT 4 cut(s) 290, 664, 711, 935
SsiI CCGC 1 cut(s) 852
SspI AATATT 2 cut(s) 235, 259
SspMI CTAG 3 cut(s) 77, 287, 795
SstI GAGCTC 2 cut(s) 167, 756
StyI CCWWGG 2 cut(s) 336, 473
TaaI ACNGT 2 cut(s) 401, 422
TaqI TCGA 3 cut(s) 41, 247, 543
TasI AATT 4 cut(s) 290, 664, 711, 935
TfiI GAWTC 2 cut(s) 94, 157
Tru1I TTAA 3 cut(s) 191, 231, 714
Tru9I TTAA 3 cut(s) 191, 231, 714
TseI GCWGC 2 cut(s) 329, 513
TspDTI ATGAA 3 cut(s) 944, 962, 975
TspGWI ACGGA 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 935
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 1 cut(s) 594
XspI CTAG 3 cut(s) 77, 287, 795
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.