Rorug03G0244200

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011672
Physical Location & Seq
Reverse (-)
22814186 .. 22816350
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0244200.1

Sequence Viewer

Length: 747 bp
ATGGCGGAAGGAGGAGGGGAAGAAGATGACTACATGGGGGACCTCTCTCGATTTGTCTCTCCAGAAGCTTCCGTTCCTCTCTCGAAAAAGGTATCGAATAGCAAAGCCTCCGGTGTTCAAGCCGCTAAGAAAAAACCCAAAGCAGTGAATTGGCAAGAGCAGCGTAAGATTGATAGGGAGAGAAAGCAACGAGAGGAGGATGAGAAAACGTTGGCGAATATAGAGGCGCCTATTCCGCAATCCAACATAGGGTTCAAGCTTTTGAAGCAAATGGGGTATACCCCAGGGTCTGCCCTTGGGAAGGAGGGGTCGGGCCGGGCTGAACCGGTGAAGGTTGATATCCGGCGGTCGAGAGCTGGAATTGGGAGGGAGGATCCTCATAAGGAGAAGAGGAAGAGAGAGGAGGTGAGGAGTGAGGAGGAGAAGAGGAATGAGGAGAGCTTGATGGAGGAGTTTGGGTGGAGGAAGAAGTCACAGTGGCGGATTCGGAGAGTTGTGGTTAATTTTAACAAGGCCAAGGCGGTTTTGGATCAGTTGGAGAATAAGGAGGTTGTGCCGAATGAGGATGAGGAAGAGGAGGGGGGAGAGTCGGAAGAGGAAGAGGAACAGATTACAGAAGAGGATTTACAAGAGATATTGATGAAACTGAGAGATGAACATCGATACTGCCTCTTCTGTGGATGCCAGTATGAATCAATGGAGGAACTATTATCCAGCTGCCCTGGAACTAATGAAGACGATCACTGA

Protein Analysis

248

Amino Acids

28.5

Weight (kDa)

5.04

Isoelectric Point (pI)

79.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 76 - 124 5.4e-07 G-patch domain
G-patch PF01585 81 - 123 8.9e-16 G-patch domain
DUF4187 PF13821 196 - 248 3.2e-19 Domain of unknown function (DUF4187)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011672)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66250
fragaria_vesca FvH4_1g30380 FvH4_1g30380 FvH4_1g30380 FvH4_1g30380 FvH4_1g30380
malus_domestica MD15G1319500.v1.1
prunus_persica Prupe.3G016900_v2.0.a1
pyrus_communis pycom15g28090
rosa_chinensis RchiOBHm_Chr3g0490581
rosa_multiflora Rmu_sc0003862.1_g000003
rosa_roxburghii Rroxscaffold_6G00393400
rosa_rugosa Rorug03G0244100 Rorug03G0244200
rosa_samantha Rh3AG293800 Rh3BG330100 Rh3CG327500 Rh3DG326800
rosa_wichuraiana Rw3G025980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 278
AciI CCGC 6 cut(s) 5, 123, 236, 346, 481, 521
AclI AACGTT 1 cut(s) 209
AclWI GGATC 3 cut(s) 368, 381, 537
AcyI GRCGYC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 249, 301
AgeI ACCGGT 1 cut(s) 325
AgsI TTSAA 3 cut(s) 119, 256, 265
AjnI CCWGG 2 cut(s) 283, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 5 cut(s) 68, 259, 356, 441, 717
AluI AGCT 5 cut(s) 68, 259, 356, 441, 717
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 3 cut(s) 368, 381, 537
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 2 cut(s) 313, 513
ApeKI GCWGC 2 cut(s) 160, 717
AsiGI ACCGGT 1 cut(s) 325
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 2 cut(s) 40, 313
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 340, 418
AvaII GGWCC 1 cut(s) 40
BamHI GGATCC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 226
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
BbsI GAAGAC 1 cut(s) 741
BbvI GCAGC 2 cut(s) 172, 704
BccI CCATC 1 cut(s) 439
BciT130I CCWGG 2 cut(s) 285, 723
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 230
BisI GCNGC 3 cut(s) 123, 161, 718
BlsI GCNGC 3 cut(s) 124, 162, 719
Bme1390I CCNGG 3 cut(s) 285, 317, 723
Bme18I GGWCC 1 cut(s) 40
BmgT120I GGNCC 2 cut(s) 40, 313
BmiI GGNNCC 3 cut(s) 41, 228, 375
BmrFI CCNGG 3 cut(s) 285, 317, 723
BmsI GCATC 1 cut(s) 671
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 661
BsaBI GATNNNNATC 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 5 cut(s) 283, 284, 295, 516, 721
BsaWI WCCGGW 2 cut(s) 110, 325
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 301
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 685
Bse8I GATNNNNATC 1 cut(s) 657
BseBI CCWGG 2 cut(s) 285, 723
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 5 cut(s) 283, 284, 295, 516, 721
BseGI GGATG 3 cut(s) 205, 571, 686
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 2 cut(s) 249, 301
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 9 cut(s) 27, 209, 416, 424, 431, 434, 449, 464, 590
BseXI GCAGC 2 cut(s) 172, 704
Bsh1285I CGRYCG 1 cut(s) 350
BshFI GGCC 2 cut(s) 315, 515
BshNI GGYRCC 1 cut(s) 226
BshTI ACCGGT 1 cut(s) 325
BshVI ATCGAT 1 cut(s) 661
BsiEI CGRYCG 1 cut(s) 350
BsiSI CCGG 4 cut(s) 111, 316, 326, 343
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 249, 301
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 2 cut(s) 315, 515
Bsp143I GATC 3 cut(s) 373, 529, 739
BspACI CCGC 6 cut(s) 5, 123, 236, 346, 481, 521
BspANI GGCC 2 cut(s) 315, 515
BspCNI CTCAG 1 cut(s) 639
BspDI ATCGAT 1 cut(s) 661
BspLI GGNNCC 3 cut(s) 41, 228, 375
BspPI GGATC 3 cut(s) 368, 381, 537
BspT107I GGYRCC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 1 cut(s) 685
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 5 cut(s) 283, 284, 295, 516, 721
BssMI GATC 3 cut(s) 373, 529, 739
BssNAI GTATAC 1 cut(s) 279
BssNI GRCGYC 1 cut(s) 227
BssT1I CCWWGG 2 cut(s) 295, 516
Bst1107I GTATAC 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 285, 723
Bst4CI ACNGT 1 cut(s) 477
Bst6I CTCTTC 8 cut(s) 383, 389, 419, 567, 588, 594, 612, 677
BstACI GRCGYC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 126, 647
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 3 cut(s) 205, 571, 686
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 3 cut(s) 376, 532, 742
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 3 cut(s) 373, 529, 739
BstMCI CGRYCG 1 cut(s) 350
BstMWI GCNNNNNNNGC 3 cut(s) 160, 235, 265
BstNI CCWGG 2 cut(s) 285, 723
BstSCI CCNGG 3 cut(s) 283, 315, 721
BstV1I GCAGC 2 cut(s) 172, 704
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BstZ17I GTATAC 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 661
BsuRI GGCC 2 cut(s) 315, 515
BsuTUI ATCGAT 1 cut(s) 661
BtsCI GGATG 3 cut(s) 205, 571, 686
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 3 cut(s) 150, 482, 742
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 40, 313
ClaI ATCGAT 1 cut(s) 661
CspAI ACCGGT 1 cut(s) 325
CviAII CATG 1 cut(s) 34
DdeI CTNAG 2 cut(s) 126, 647
DinI GGCGCC 1 cut(s) 228
DpnI GATC 3 cut(s) 375, 531, 741
DpnII GATC 3 cut(s) 373, 529, 739
Eam1104I CTCTTC 8 cut(s) 383, 389, 419, 567, 588, 594, 612, 677
EarI CTCTTC 8 cut(s) 383, 389, 419, 567, 588, 594, 612, 677
EciI GGCGGA 2 cut(s) 20, 496
Eco130I CCWWGG 2 cut(s) 295, 516
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 40
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 283, 721
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 295, 516
EgeI GGCGCC 1 cut(s) 228
EheI GGCGCC 1 cut(s) 228
ErhI CCWWGG 2 cut(s) 295, 516
FaeI CATG 1 cut(s) 37
FaiI YATR 6 cut(s) 35, 221, 248, 279, 381, 690
FaqI GGGAC 1 cut(s) 53
FatI CATG 1 cut(s) 33
FblI GTMKAC 1 cut(s) 278
Fnu4HI GCNGC 3 cut(s) 123, 161, 718
FokI GGATG 3 cut(s) 212, 578, 693
Fsp4HI GCNGC 3 cut(s) 123, 161, 718
GlaI GCGC 1 cut(s) 228
GluI GCNGC 3 cut(s) 123, 161, 718
GsuI CTGGAG 1 cut(s) 45
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 2 cut(s) 315, 515
HapII CCGG 4 cut(s) 111, 316, 326, 343
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 1 cut(s) 37
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HindIII AAGCTT 2 cut(s) 66, 257
HinfI GANTC 3 cut(s) 484, 587, 692
HpaII CCGG 4 cut(s) 111, 316, 326, 343
HphI GGTGA 2 cut(s) 340, 418
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 2 cut(s) 489, 592
Hpy188III TCNNGA 4 cut(s) 48, 62, 82, 351
Hpy8I GTNNAC 1 cut(s) 279
HpyAV CCTTC 2 cut(s) 295, 325
HpyCH4III ACNGT 1 cut(s) 477
HpyCH4IV ACGT 1 cut(s) 209
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 235, 265
HpyF3I CTNAG 2 cut(s) 126, 647
HpySE526I ACGT 1 cut(s) 209
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 1 cut(s) 37
HspAI GCGC 1 cut(s) 227
KasI GGCGCC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 373, 529, 739
Lsp1109I GCAGC 2 cut(s) 172, 704
LweI GCATC 1 cut(s) 671
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 471
MalI GATC 3 cut(s) 375, 531, 741
MboI GATC 3 cut(s) 373, 529, 739
MflI RGATCY 1 cut(s) 373
MluCI AATT 3 cut(s) 148, 360, 502
Mly113I GGCGCC 1 cut(s) 227
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 3 cut(s) 267, 516, 570
MseI TTAA 2 cut(s) 501, 507
MspA1I CMGCKG 1 cut(s) 717
MspI CCGG 4 cut(s) 111, 316, 326, 343
MspR9I CCNGG 3 cut(s) 285, 317, 723
MvaI CCWGG 2 cut(s) 285, 723
MwoI GCNNNNNNNGC 3 cut(s) 160, 235, 265
NarI GGCGCC 1 cut(s) 227
NciI CCSGG 1 cut(s) 317
NdeII GATC 3 cut(s) 373, 529, 739
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 3 cut(s) 41, 228, 375
NmuCI GTSAC 1 cut(s) 471
PasI CCCWGGG 1 cut(s) 284
PfeI GAWTC 2 cut(s) 484, 692
PinAI ACCGGT 1 cut(s) 325
PkrI GCNGC 3 cut(s) 124, 162, 719
PleI GAGTC 1 cut(s) 595
PluTI GGCGCC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 40
Psp1406I AACGTT 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 283, 721
PspGI CCWGG 2 cut(s) 283, 721
PspN4I GGNNCC 3 cut(s) 41, 228, 375
PspPI GGNCC 2 cut(s) 40, 313
PspPPI RGGWCCY 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 373
PvuII CAGCTG 1 cut(s) 717
SaqAI TTAA 2 cut(s) 501, 507
SatI GCNGC 3 cut(s) 123, 161, 718
Sau3AI GATC 3 cut(s) 373, 529, 739
Sau96I GGNCC 2 cut(s) 40, 313
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 3 cut(s) 285, 317, 723
SfaNI GCATC 1 cut(s) 671
SfoI GGCGCC 1 cut(s) 228
SinI GGWCC 1 cut(s) 40
Sse9I AATT 3 cut(s) 148, 360, 502
SsiI CCGC 6 cut(s) 5, 123, 236, 346, 481, 521
SspDI GGCGCC 1 cut(s) 226
StyD4I CCNGG 3 cut(s) 283, 315, 721
StyI CCWWGG 2 cut(s) 295, 516
TaaI ACNGT 1 cut(s) 477
TaiI ACGT 1 cut(s) 212
TaqI TCGA 5 cut(s) 49, 83, 95, 350, 661
TasI AATT 3 cut(s) 148, 360, 502
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 2 cut(s) 484, 692
Tru1I TTAA 2 cut(s) 501, 507
Tru9I TTAA 2 cut(s) 501, 507
TscAI CASTG 2 cut(s) 150, 482
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 2 cut(s) 160, 717
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 4 cut(s) 656, 669, 705, 747
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 2 cut(s) 150, 482
VpaK11BI GGWCC 1 cut(s) 40
XagI CCTNNNNNAGG 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.