Rh3DG326800

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011672
Physical Location & Seq
Reverse (-)
35901181 .. 35904981
3801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG326800.1

Sequence Viewer

Length: 1368 bp
ATGGCTCTGAAGCTTCTGCTTTTACTTTTAGCTATTTCAGTAGCTACCGCTGATGAAGATGTATTTATTGGTGTGAATATAGGAACAGCCCTCTCCGAAATGCCACACCCAACTCAAGTAGTAGCGCTTTTAAAAGCCCAGCAAATTCGACATGTCCGGTTATATGATGCAGATCGTGCAATGCTTGTGGCACTTGCAAATACAGGCATAAAAGTCATTATTTCTGTGCCTAATGATCAGATCCTTGGAATTGGTCAATCTAATTCCACTGCAGCTAATTGGGTATCCCAGAATGTAGTAGCACATTACCCAGCTACAAACATCACAGCCATATCTGTTGGCTCAGAGGTCCTTACTACCCTCCCAAATGCTGCAAAAATCCTCGTCAGTGCACTCAAGTACATTCACTCAGCCCTTGTGGCATCCAATCTTGACAGCCAAGTCAAAGTTTCTACTCCTCTTTCTTCCTCCATAATCCTTGATTCATTCCCACCTTCCCAAGCCTTCTTTAACCGCTCACTGAATCCAGTTTTGGTCCCCATGCTTAGTTTCTTGCAGTCCACGGGCTCGTTTCTGATGCTTAATGTATATCCATACTATGACTACATGCAATCAAATGGTGTTATTGCATTGGATTATGCACTGCTCAACCCTCTCCAGGCAAACAAAGAAGCTGTTGATTCGAATACGCTTCTCCATTATTCTAATGTTTTTGATGCTGTGGTTGATGCAGCATATTATGCCATGGCTTTTCTTAACTTCACCAATGTTCCAGTAGTAGTGACAGAATCAGGCTGGCCCTCAAAAGGTGATTCTAATGAGCCAGACGCAACATTAGAAAATGCGAACACTTACAACAGCAATTTAATAAGGCACGTGTTGAACAAGACTGGAACTCCCAAACATCCTGGACTTGCTGTTAGTACTTTCATTTATGAACTCTATAATGAGGATACAAAACCTGGGCCTGTCTCAGAGAAGAACTGGGGATTGTTTGATGCAAATGGAACACCTATCTACATATTACGGTTGACGGGATCAGGAGCAGTATTAGCAAATGATACTACAAATCAAACTTTCTGTACAGTAAAGGATGGCACTGATACAAAGATGCTTCAGGCTGCACTAGATTGGGCTTGTGGACCTGGCAAGGTGGATTGCTCACCATTATTGCAGGGAGAACCCTGCTACGAACCAGATAATGTGATTTCACACGCAACGTATGCGTTTGACACTTATTATCATCAGATGGGGAAGACGCCTGCTGCATGTGACTTCAATGGAGTGGCTGCAATCACCACTACCGATCCAAGTAAGATCTCAACTTCACAGCATGCAGTATGCTCTGACTTTTTATATTCTATTTGA

Protein Analysis

455

Amino Acids

49.0

Weight (kDa)

4.95

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 23 - 342 2.2e-78 Glycosyl hydrolases family 17
X8 PF07983 360 - 430 1.4e-18 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011672)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66250
fragaria_vesca FvH4_1g30380 FvH4_1g30380 FvH4_1g30380 FvH4_1g30380 FvH4_1g30380
malus_domestica MD15G1319500.v1.1
prunus_persica Prupe.3G016900_v2.0.a1
pyrus_communis pycom15g28090
rosa_chinensis RchiOBHm_Chr3g0490581
rosa_multiflora Rmu_sc0003862.1_g000003
rosa_roxburghii Rroxscaffold_6G00393400
rosa_rugosa Rorug03G0244100 Rorug03G0244200
rosa_samantha Rh3AG293800 Rh3BG330100 Rh3CG327500 Rh3DG326800
rosa_wichuraiana Rw3G025980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
AccBSI CCGCTC 1 cut(s) 516
AciI CCGC 2 cut(s) 48, 514
AclWI GGATC 3 cut(s) 235, 1045, 1301
AcsI RAATTY 1 cut(s) 144
AcuI CTGAAG 2 cut(s) 29, 1100
AcvI CACGTG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 1259
AfaI GTAC 3 cut(s) 401, 925, 1084
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 658, 806
AflIII ACRYGT 2 cut(s) 151, 876
AgsI TTSAA 2 cut(s) 883, 1279
AjnI CCWGG 4 cut(s) 657, 907, 961, 1144
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
AluBI AGCT 6 cut(s) 13, 32, 44, 275, 314, 674
AluI AGCT 6 cut(s) 13, 32, 44, 275, 314, 674
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 1 cut(s) 976
Alw44I GTGCAC 1 cut(s) 390
AlwI GGATC 3 cut(s) 235, 1045, 1301
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 2 cut(s) 797, 965
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 6 cut(s) 272, 371, 731, 1121, 1265, 1289
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 5 cut(s) 349, 535, 798, 965, 1142
AsuHPI GGTGA 4 cut(s) 754, 821, 1155, 1288
AsuII TTCGAA 1 cut(s) 683
AvaII GGWCC 3 cut(s) 349, 535, 1142
BaeGI GKGCMC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 569
BbrPI CACGTG 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 1262
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 6 cut(s) 284, 358, 743, 1108, 1252, 1276
BccI CCATC 2 cut(s) 1088, 1243
BciT130I CCWGG 4 cut(s) 659, 909, 963, 1146
BciVI GTATCC 2 cut(s) 295, 946
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 976
BfaI CTAG 1 cut(s) 1127
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 128
BfuI GTATCC 2 cut(s) 295, 946
BglI GCCNNNNNGGC 1 cut(s) 419
BglII AGATCT 1 cut(s) 1317
BisI GCNGC 6 cut(s) 273, 372, 732, 1122, 1266, 1290
BlsI GCNGC 6 cut(s) 274, 373, 733, 1123, 1267, 1291
BmcAI AGTACT 1 cut(s) 925
Bme1390I CCNGG 4 cut(s) 659, 909, 963, 1146
Bme18I GGWCC 3 cut(s) 349, 535, 1142
BmgT120I GGNCC 5 cut(s) 349, 535, 798, 965, 1142
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 4 cut(s) 659, 909, 963, 1146
BmrI ACTGGG 1 cut(s) 994
BmsI GCATC 7 cut(s) 157, 431, 567, 706, 718, 988, 1101
BmuI ACTGGG 1 cut(s) 994
BpiI GAAGAC 1 cut(s) 1262
BpmI CTGGAG 1 cut(s) 641
Bpu14I TTCGAA 1 cut(s) 683
BpuEI CTTGAG 2 cut(s) 99, 380
BsaAI YACGTR 1 cut(s) 877
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 1259
BsaJI CCNNGG 4 cut(s) 244, 561, 744, 962
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 880, 910
Bsc4I CCNNNNNNNGG 2 cut(s) 658, 806
Bse1I ACTGG 4 cut(s) 527, 773, 895, 989
Bse3DI GCAATG 1 cut(s) 186
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 4 cut(s) 659, 909, 963, 1146
BseDI CCNNGG 4 cut(s) 244, 561, 744, 962
BseGI GGATG 3 cut(s) 422, 904, 1099
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 658, 806
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 357, 423, 987
BseNI ACTGG 4 cut(s) 527, 773, 895, 989
BseRI GAGGAG 1 cut(s) 447
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 6 cut(s) 284, 358, 743, 1108, 1252, 1276
BseYI CCCAGC 2 cut(s) 138, 310
BsgI GTGCAG 1 cut(s) 1107
BshFI GGCC 2 cut(s) 799, 967
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 157
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 2 cut(s) 658, 806
BsmAI GTCTC 1 cut(s) 976
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 2 cut(s) 799, 967
Bsp119I TTCGAA 1 cut(s) 683
Bsp1286I GDGCHC 2 cut(s) 394, 569
Bsp1407I TGTACA 1 cut(s) 1082
Bsp143I GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 2 cut(s) 48, 514
BspANI GGCC 2 cut(s) 799, 967
BspCNI CTCAG 3 cut(s) 356, 422, 986
BspLI GGNNCC 1 cut(s) 537
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 3 cut(s) 235, 1045, 1301
BspT104I TTCGAA 1 cut(s) 683
BsrBI CCGCTC 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 1082
BsrI ACTGG 4 cut(s) 527, 773, 895, 989
BssECI CCNNGG 4 cut(s) 244, 561, 744, 962
BssMI GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
BssNI GRCGYC 1 cut(s) 1259
BssT1I CCWWGG 2 cut(s) 244, 744
Bst2UI CCWGG 4 cut(s) 659, 909, 963, 1146
Bst4CI ACNGT 2 cut(s) 1029, 1087
BstACI GRCGYC 1 cut(s) 1259
BstAPI GCANNNNNTGC 3 cut(s) 176, 740, 1223
BstAUI TGTACA 1 cut(s) 1082
BstBAI YACGTR 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 683
BstC8I GCNNGC 3 cut(s) 797, 1263, 1335
BstDEI CTNAG 4 cut(s) 343, 409, 545, 973
BstDSI CCRYGG 2 cut(s) 561, 744
BstF5I GGATG 3 cut(s) 422, 904, 1099
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 6 cut(s) 175, 238, 243, 1040, 1309, 1320
BstMAI GTCTC 1 cut(s) 976
BstMBI GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
BstMWI GCNNNNNNNGC 5 cut(s) 176, 419, 740, 1052, 1223
BstNI CCWGG 4 cut(s) 659, 909, 963, 1146
BstNSI RCATGY 4 cut(s) 155, 610, 1272, 1337
BstSCI CCNGG 4 cut(s) 657, 907, 961, 1144
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 6 cut(s) 284, 358, 743, 1108, 1252, 1276
BstV2I GAAGAC 1 cut(s) 1262
BstX2I RGATCY 2 cut(s) 240, 1317
BstYI RGATCY 2 cut(s) 240, 1317
BsuI GTATCC 2 cut(s) 295, 946
BsuRI GGCC 2 cut(s) 799, 967
BtgI CCRYGG 2 cut(s) 561, 744
BtsCI GGATG 3 cut(s) 422, 904, 1099
BtsI GCAGTG 2 cut(s) 267, 641
BtsIMutI CAGTG 5 cut(s) 267, 394, 518, 641, 1098
Cac8I GCNNGC 3 cut(s) 797, 1263, 1335
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 5 cut(s) 349, 535, 798, 965, 1142
CseI GACGC 2 cut(s) 836, 1267
Csp6I GTAC 3 cut(s) 400, 924, 1083
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviAII CATG 6 cut(s) 152, 541, 607, 745, 1269, 1334
CviQI GTAC 3 cut(s) 400, 924, 1083
DdeI CTNAG 4 cut(s) 343, 409, 545, 973
DpnI GATC 6 cut(s) 174, 237, 242, 1039, 1308, 1319
DpnII GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
DraI TTTAAA 1 cut(s) 132
DrdI GACNNNNNNGTC 1 cut(s) 440
DseDI GACNNNNNNGTC 1 cut(s) 440
Eco130I CCWWGG 2 cut(s) 244, 744
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 3 cut(s) 349, 535, 1142
Eco47III AGCGCT 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 29, 1100
Eco72I CACGTG 1 cut(s) 877
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 4 cut(s) 657, 907, 961, 1144
EcoT14I CCWWGG 2 cut(s) 244, 744
EcoT38I GRGCYC 1 cut(s) 569
ErhI CCWWGG 2 cut(s) 244, 744
FaeI CATG 6 cut(s) 155, 544, 610, 748, 1272, 1337
FaqI GGGAC 1 cut(s) 521
FatI CATG 6 cut(s) 151, 540, 606, 744, 1268, 1333
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 6 cut(s) 273, 372, 732, 1122, 1266, 1290
FokI GGATG 3 cut(s) 409, 891, 1106
FriOI GRGCYC 1 cut(s) 569
Fsp4HI GCNGC 6 cut(s) 273, 372, 732, 1122, 1266, 1290
FspBI CTAG 1 cut(s) 1127
GlaI GCGC 1 cut(s) 126
GluI GCNGC 6 cut(s) 273, 372, 732, 1122, 1266, 1290
GsaI CCCAGC 2 cut(s) 142, 314
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 2 cut(s) 799, 967
HapII CCGG 1 cut(s) 157
HgaI GACGC 2 cut(s) 836, 1267
HhaI GCGC 1 cut(s) 127
Hin1I GRCGYC 1 cut(s) 1259
Hin1II CATG 6 cut(s) 155, 544, 610, 748, 1272, 1337
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 1032
HindII GTYRAC 1 cut(s) 1032
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 5 cut(s) 482, 523, 680, 788, 812
HpaII CCGG 1 cut(s) 157
HphI GGTGA 4 cut(s) 754, 821, 1155, 1288
Hpy166II GTNNAC 4 cut(s) 392, 561, 1032, 1142
Hpy188I TCNGA 8 cut(s) 9, 97, 240, 346, 576, 976, 1248, 1348
Hpy188III TCNNGA 2 cut(s) 431, 1041
Hpy8I GTNNAC 4 cut(s) 392, 561, 1032, 1142
HpyAV CCTTC 2 cut(s) 504, 514
HpyCH4III ACNGT 2 cut(s) 1029, 1087
HpyCH4IV ACGT 2 cut(s) 876, 1220
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 419, 740, 1052, 1223
HpyF3I CTNAG 4 cut(s) 343, 409, 545, 973
HpySE526I ACGT 2 cut(s) 876, 1220
Hsp92I GRCGYC 1 cut(s) 1259
Hsp92II CATG 6 cut(s) 155, 544, 610, 748, 1272, 1337
HspAI GCGC 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
LmnI GCTCC 1 cut(s) 1043
Lsp1109I GCAGC 6 cut(s) 284, 358, 743, 1108, 1252, 1276
LweI GCATC 7 cut(s) 157, 431, 567, 706, 718, 988, 1101
MaeI CTAG 1 cut(s) 1127
MaeII ACGT 2 cut(s) 876, 1220
MaeIII GTNAC 2 cut(s) 781, 1271
MalI GATC 6 cut(s) 174, 237, 242, 1039, 1308, 1319
MbiI CCGCTC 1 cut(s) 516
MboI GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
MboII GAAGA 4 cut(s) 68, 456, 991, 1267
MflI RGATCY 2 cut(s) 240, 1317
MhlI GDGCHC 2 cut(s) 394, 569
MluCI AATT 5 cut(s) 144, 249, 262, 277, 862
MnlI CCTC 9 cut(s) 101, 340, 371, 392, 468, 478, 663, 811, 943
MseI TTAA 5 cut(s) 131, 510, 582, 756, 866
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 4 cut(s) 659, 909, 963, 1146
MvaI CCWGG 4 cut(s) 659, 909, 963, 1146
MwoI GCNNNNNNNGC 5 cut(s) 176, 419, 740, 1052, 1223
NcoI CCATGG 1 cut(s) 744
NdeII GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
NlaIII CATG 6 cut(s) 155, 544, 610, 748, 1272, 1337
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 2 cut(s) 781, 1271
NspI RCATGY 4 cut(s) 155, 610, 1272, 1337
NspV TTCGAA 1 cut(s) 683
PaeI GCATGC 1 cut(s) 1337
PciI ACATGT 1 cut(s) 151
PfeI GAWTC 5 cut(s) 482, 523, 680, 788, 812
PfoI TCCNGGA 1 cut(s) 907
PkrI GCNGC 6 cut(s) 274, 373, 733, 1123, 1267, 1291
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
Ppu21I YACGTR 1 cut(s) 877
PpuMI RGGWCCY 1 cut(s) 349
PscI ACATGT 1 cut(s) 151
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 4 cut(s) 657, 907, 961, 1144
PspCI CACGTG 1 cut(s) 877
PspFI CCCAGC 2 cut(s) 138, 310
PspGI CCWGG 4 cut(s) 657, 907, 961, 1144
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 5 cut(s) 349, 535, 798, 965, 1142
PspPPI RGGWCCY 1 cut(s) 349
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 2 cut(s) 240, 1317
RsaI GTAC 3 cut(s) 401, 925, 1084
RsaNI GTAC 3 cut(s) 400, 924, 1083
SaqAI TTAA 5 cut(s) 131, 510, 582, 756, 866
SatI GCNGC 6 cut(s) 273, 372, 732, 1122, 1266, 1290
Sau3AI GATC 6 cut(s) 172, 235, 240, 1037, 1306, 1317
Sau96I GGNCC 5 cut(s) 349, 535, 798, 965, 1142
ScaI AGTACT 1 cut(s) 925
ScrFI CCNGG 4 cut(s) 659, 909, 963, 1146
SduI GDGCHC 2 cut(s) 394, 569
SfaNI GCATC 7 cut(s) 157, 431, 567, 706, 718, 988, 1101
SfcI CTRYAG 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 683
SinI GGWCC 3 cut(s) 349, 535, 1142
SmlI CTYRAG 2 cut(s) 114, 395
SmoI CTYRAG 2 cut(s) 114, 395
SphI GCATGC 1 cut(s) 1337
Sse9I AATT 5 cut(s) 144, 249, 262, 277, 862
SsiI CCGC 2 cut(s) 48, 514
SspMI CTAG 1 cut(s) 1127
StyD4I CCNGG 4 cut(s) 657, 907, 961, 1144
StyI CCWWGG 2 cut(s) 244, 744
TaaI ACNGT 2 cut(s) 1029, 1087
TaiI ACGT 2 cut(s) 879, 1223
TaqI TCGA 2 cut(s) 148, 683
TasI AATT 5 cut(s) 144, 249, 262, 277, 862
TatI WGTACW 3 cut(s) 399, 923, 1082
TfiI GAWTC 5 cut(s) 482, 523, 680, 788, 812
Tru1I TTAA 5 cut(s) 131, 510, 582, 756, 866
Tru9I TTAA 5 cut(s) 131, 510, 582, 756, 866
TscAI CASTG 5 cut(s) 274, 394, 525, 648, 1105
TseFI GTSAC 2 cut(s) 781, 1271
TseI GCWGC 6 cut(s) 272, 371, 731, 1121, 1265, 1289
Tsp45I GTSAC 2 cut(s) 781, 1271
TspDTI ATGAA 4 cut(s) 69, 474, 919, 951
TspRI CASTG 5 cut(s) 274, 394, 525, 648, 1105
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 3 cut(s) 349, 535, 1142
XapI RAATTY 1 cut(s) 144
XceI RCATGY 4 cut(s) 155, 610, 1272, 1337
XspI CTAG 1 cut(s) 1127
ZrmI AGTACT 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.