Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0009305

Physical Location & Seq

Genomic Coordinates Forward (+)

24781219 .. 24841958

Gene Structure 60740 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0258700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2895 bp

ATGGCTGGGGCGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGTATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATAGCTACTTGAAGTACATCAGCGAGCATGGAGGAAGAGGAAATGCTTTCACTGCTGCAGAACACACCAGCTATCGTTTCGACATTAATCCCGATGGTTTTGAAGAAGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGTGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAAATCTGTTGTCCGATGACTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGTATTCTTTGTGGGTTCGACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTAAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCGTGTACGTATCTAGGTCACCTTATTGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGCGGGCTGGTGAATCATATTCTACTTTGGACTTTTCTTTCTTTGAAGTAGATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACGTTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTATTTGTGAGACAAAGTTTCATTATCAGGACAAAATTGAGCCTTTTAATTACGTGGTCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGATAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAAAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGTGAATTTACACCTACCAGCACGTAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTGTGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTGATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATAAACCGCACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAACGGTCACAAAGGCGTACCAAAATTACAAATTTGAACAGTCCTATGAGCAGGCTATTTACTATTGCTCATTAGTTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATCGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGGCTTAATCGAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTATTTGTTCTTATTGCAAAGCAACCAGCCTTTCACCAACTTAGAATAGTTGAGCAACTTGGTTACATAACTCGTCTTTCACAGAGGAATGATTGTGGTATCCGTGGACTGCAGTTCATTATACAATCCACAGTGAAGGGTCCAGGACGTATTGATTTGAGAGTGGAGGAATTTCTCAAGACGTTTGAGAGTAAGCTTTATGAGATGACAAATAACGAATTCAAGAGCAATGTAAATACTTTGATCGATCTGAAGCTTGAGAAGCACCAGAACATGAGGGAAGAAGCTGAATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTTCAATGAGCGTATAAAAGTTGGGGCACCTCATAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATTTGAGTATGCATCAGATAAAAGCTCCCTAGTGCAACCTTGCACCGTCAAAATCGATGATATTTTCGCTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAGCTCCGGTCATGTGAAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0010731 GO:0015036 GO:0015037 GO:0015038 GO:0016209 GO:0016491 GO:0016667 GO:0016672 GO:0016740 GO:0016765 GO:0019538 GO:0031967 GO:0031975 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045174 GO:0050896 GO:0051186 GO:0051716 GO:0055114 GO:0070887 GO:0071704 GO:0097237 GO:0098754 GO:0098869 GO:1901564 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko00053 ko00480 ko01100 map00053 map00480 map01100

Pfam Domains

Protein Families

GST_C_2 GST_N_3

Protein Analysis

964

Amino Acids

111.27

Weight (kDa)

6.24

Isoelectric Point (pI)

36.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	29 - 163	6.8e-32	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	191 - 368	2.2e-28	Peptidase M16 inactive domain
Peptidase_M16_M	PF16187	376 - 654	1.1e-102	Middle or third domain of peptidase_M16
Peptidase_M16_C	PF05193	659 - 845	5.9e-11	Peptidase M16 inactive domain
PqqF-like_C_4	PF22456	770 - 865	4.3e-20	PQQ synthase PqqF-like, C-terminal lobe domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16710
fragaria_vesca	FvH4_1g28700 FvH4_1g28700 FvH4_1g28700 FvH4_1g28700
malus_domestica	MD09G1264700.v1.1 MD17G1260600.v1.1
prunus_persica	Prupe.3G162300_v2.0.a1 Prupe.3G162300_v2.0.a1
pyrus_communis	pycom09g17830 pycom17g26130
rosa_chinensis	RchiOBHm_Chr3g0493051
rosa_laevigata	RLG00000022732
rosa_multiflora	Rmu_sc0003941.1_g000013
rosa_roxburghii	Rroxscaffold_6G00391890
rosa_rugosa	Rorug03G0258700 Rorug03G0258700
rosa_samantha	Rh3AG307800 Rh3BG343200 Rh3CG340600 Rh3DG343000
rosa_wichuraiana	Rw3G027070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1758
AccB1I	GGYRCC	1 cut(s)	2713
AciI	CCGC	2 cut(s)	940, 1720
AclWI	GGATC	4 cut(s)	68, 95, 901, 2074
AcoI	YGGCCR	2 cut(s)	877, 1940
AcuI	CTGAAG	5 cut(s)	458, 1222, 2085, 2276, 2567
AfiI	CCNNNNNNNGG	7 cut(s)	232, 456, 676, 841, 1349, 2207, 2855
AflII	CTTAAG	1 cut(s)	1472
AflIII	ACRYGT	1 cut(s)	1332
AhlI	ACTAGT	1 cut(s)	1634
AjnI	CCWGG	4 cut(s)	168, 1343, 2253, 2437
AjuI	GAANNNNNNNTTGG	2 cut(s)	212, 244
Alw26I	GTCTC	2 cut(s)	1112, 1473
AlwI	GGATC	4 cut(s)	68, 95, 901, 2074
AoxI	GGCC	9 cut(s)	166, 175, 667, 812, 848, 877, 1009, 1940, 2022
ApeKI	GCWGC	5 cut(s)	122, 290, 491, 2648, 2659
AseI	ATTAAT	1 cut(s)	321
Asp700I	GAANNNNTTC	1 cut(s)	1965
AspS9I	GGNCC	6 cut(s)	175, 848, 1930, 2022, 2435, 2613
AsuC2I	CCSGG	1 cut(s)	1616
AsuHPI	GGTGA	9 cut(s)	743, 824, 860, 959, 991, 1334, 1509, 1753, 2321
AsuII	TTCGAA	1 cut(s)	697
AvaII	GGWCC	3 cut(s)	1930, 2435, 2613
BaeGI	GKGCMC	1 cut(s)	2716
BalI	TGGCCA	2 cut(s)	879, 1942
BanI	GGYRCC	1 cut(s)	2713
BanII	GRGCYC	2 cut(s)	1344, 1385
BbvCI	CCTCAGC	1 cut(s)	1689
BbvI	GCAGC	5 cut(s)	109, 277, 503, 2660, 2671
BccI	CCATC	6 cut(s)	158, 323, 918, 1942, 2149, 2603
BceAI	ACGGC	1 cut(s)	835
BciT130I	CCWGG	4 cut(s)	170, 1345, 2255, 2439
BciVI	GTATCC	2 cut(s)	562, 2405
BclI	TGATCA	1 cut(s)	1839
BcnI	CCSGG	1 cut(s)	1616
BcoDI	GTCTC	2 cut(s)	1112, 1473
BcuI	ACTAGT	1 cut(s)	1634
BfaI	CTAG	5 cut(s)	213, 863, 1635, 1958, 2792
BfmI	CTRYAG	4 cut(s)	291, 396, 1925, 2405
BfrI	CTTAAG	1 cut(s)	1472
BfuI	GTATCC	2 cut(s)	562, 2405
BglI	GCCNNNNNGGC	1 cut(s)	174
BglII	AGATCT	1 cut(s)	1987
BisI	GCNGC	5 cut(s)	123, 291, 492, 2649, 2660
BlsI	GCNGC	5 cut(s)	124, 292, 493, 2650, 2661
BmcAI	AGTACT	1 cut(s)	2062
Bme1390I	CCNGG	5 cut(s)	170, 1345, 1616, 2255, 2439
Bme18I	GGWCC	3 cut(s)	1930, 2435, 2613
BmgT120I	GGNCC	6 cut(s)	175, 848, 1930, 2022, 2435, 2613
BmiI	GGNNCC	6 cut(s)	1384, 1612, 2436, 2614, 2615, 2715
BmrFI	CCNGG	5 cut(s)	170, 1345, 1616, 2255, 2439
BmsI	GCATC	7 cut(s)	382, 490, 634, 994, 1415, 2001, 2783
BpmI	CTGGAG	5 cut(s)	191, 478, 776, 807, 1369
Bpu10I	CCTNAGC	1 cut(s)	1689
Bpu14I	TTCGAA	1 cut(s)	697
BpuEI	CTTGAG	5 cut(s)	212, 2002, 2456, 2573, 2603
BpuMI	CCSGG	1 cut(s)	1616
Bsa29I	ATCGAT	2 cut(s)	2541, 2817
BsaAI	YACGTR	4 cut(s)	858, 1162, 1442, 2089
BsaBI	GATNNNNATC	1 cut(s)	993
BsaJI	CCNNGG	5 cut(s)	129, 1343, 1458, 1614, 2398
BsaWI	WCCGGW	1 cut(s)	2876
Bsc4I	CCNNNNNNNGG	7 cut(s)	232, 456, 676, 841, 1349, 2207, 2855
Bse1I	ACTGG	5 cut(s)	461, 733, 1211, 1499, 1785
Bse3DI	GCAATG	2 cut(s)	1223, 2530
Bse8I	GATNNNNATC	1 cut(s)	993
BseBI	CCWGG	4 cut(s)	170, 1345, 2255, 2439
BseCI	ATCGAT	2 cut(s)	2541, 2817
BseDI	CCNNGG	5 cut(s)	129, 1343, 1458, 1614, 2398
BseGI	GGATG	5 cut(s)	139, 499, 929, 1452, 2141
BseJI	GATNNNNATC	1 cut(s)	993
BseLI	CCNNNNNNNGG	7 cut(s)	232, 456, 676, 841, 1349, 2207, 2855
BseMI	GCAATG	2 cut(s)	1223, 2530
BseMII	CTCAG	4 cut(s)	374, 1680, 2061, 2645
BseNI	ACTGG	5 cut(s)	461, 733, 1211, 1499, 1785
BseRI	GAGGAG	1 cut(s)	80
BseSI	GKGCMC	1 cut(s)	2716
BseXI	GCAGC	5 cut(s)	109, 277, 503, 2660, 2671
BseYI	CCCAGC	1 cut(s)	5
BsgI	GTGCAG	2 cut(s)	510, 2069
BshFI	GGCC	9 cut(s)	168, 177, 669, 814, 850, 879, 1011, 1942, 2024
BshNI	GGYRCC	1 cut(s)	2713
BshVI	ATCGAT	2 cut(s)	2541, 2817
BsiSI	CCGG	2 cut(s)	1616, 2877
BsiWI	CGTACG	1 cut(s)	35
BslFI	GGGAC	3 cut(s)	761, 1581, 2626
BslI	CCNNNNNNNGG	7 cut(s)	232, 456, 676, 841, 1349, 2207, 2855
BsmAI	GTCTC	2 cut(s)	1112, 1473
BsmFI	GGGAC	3 cut(s)	761, 1581, 2626
BsnI	GGCC	9 cut(s)	168, 177, 669, 814, 850, 879, 1011, 1942, 2024
Bsp119I	TTCGAA	1 cut(s)	697
Bsp1286I	GDGCHC	3 cut(s)	1344, 1385, 2716
Bsp19I	CCATGG	1 cut(s)	129
BspACI	CCGC	2 cut(s)	940, 1720
BspANI	GGCC	9 cut(s)	168, 177, 669, 814, 850, 879, 1011, 1942, 2024
BspCNI	CTCAG	4 cut(s)	373, 1681, 2062, 2646
BspDI	ATCGAT	2 cut(s)	2541, 2817
BspLI	GGNNCC	6 cut(s)	1384, 1612, 2436, 2614, 2615, 2715
BspMAI	CTGCAG	2 cut(s)	295, 2409
BspPI	GGATC	4 cut(s)	68, 95, 901, 2074
BspT104I	TTCGAA	1 cut(s)	697
BspT107I	GGYRCC	1 cut(s)	2713
BspTI	CTTAAG	1 cut(s)	1472
BsrDI	GCAATG	2 cut(s)	1223, 2530
BsrI	ACTGG	5 cut(s)	461, 733, 1211, 1499, 1785
BssECI	CCNNGG	5 cut(s)	129, 1343, 1458, 1614, 2398
BssT1I	CCWWGG	1 cut(s)	129
Bst2UI	CCWGG	4 cut(s)	170, 1345, 2255, 2439
Bst4CI	ACNGT	9 cut(s)	400, 775, 1533, 1704, 1789, 1852, 1887, 2428, 2809
Bst6I	CTCTTC	2 cut(s)	12, 265
BstAFI	CTTAAG	1 cut(s)	1472
BstAPI	GCANNNNNTGC	1 cut(s)	2645
BstBAI	YACGTR	4 cut(s)	858, 1162, 1442, 2089
BstBI	TTCGAA	1 cut(s)	697
BstC8I	GCNNGC	6 cut(s)	260, 942, 1009, 1040, 1807, 1899
BstDEI	CTNAG	8 cut(s)	360, 485, 1689, 1995, 2029, 2070, 2336, 2654
BstDSI	CCRYGG	3 cut(s)	129, 1458, 2398
BstEII	GGTNACC	1 cut(s)	866
BstENI	CCTNNNNNAGG	2 cut(s)	674, 2205
BstF5I	GGATG	5 cut(s)	139, 499, 929, 1452, 2141
BstMAI	GTCTC	2 cut(s)	1112, 1473
BstMWI	GCNNNNNNNGC	8 cut(s)	174, 287, 478, 1270, 1685, 1694, 2557, 2645
BstNI	CCWGG	4 cut(s)	170, 1345, 2255, 2439
BstNSI	RCATGY	2 cut(s)	1024, 1336
BstPI	GGTNACC	1 cut(s)	866
BstSCI	CCNGG	5 cut(s)	168, 1343, 1614, 2253, 2437
BstSFI	CTRYAG	4 cut(s)	291, 396, 1925, 2405
BstSLI	GKGCMC	1 cut(s)	2716
BstSNI	TACGTA	1 cut(s)	858
BstV1I	GCAGC	5 cut(s)	109, 277, 503, 2660, 2671
BstX2I	RGATCY	2 cut(s)	893, 1987
BstXI	CCANNNNNNTGG	1 cut(s)	1607
BstYI	RGATCY	2 cut(s)	893, 1987
Bsu15I	ATCGAT	2 cut(s)	2541, 2817
BsuI	GTATCC	2 cut(s)	562, 2405
BsuRI	GGCC	9 cut(s)	168, 177, 669, 814, 850, 879, 1011, 1942, 2024
BsuTUI	ATCGAT	2 cut(s)	2541, 2817
BtgI	CCRYGG	3 cut(s)	129, 1458, 2398
BtsCI	GGATG	5 cut(s)	139, 499, 929, 1452, 2141
BtsI	GCAGTG	1 cut(s)	285
BtsIMutI	CAGTG	6 cut(s)	145, 285, 405, 1529, 2433, 2651
Cac8I	GCNNGC	6 cut(s)	260, 942, 1009, 1040, 1807, 1899
Cfr13I	GGNCC	6 cut(s)	175, 848, 1930, 2022, 2435, 2613
ClaI	ATCGAT	2 cut(s)	2541, 2817
DdeI	CTNAG	8 cut(s)	360, 485, 1689, 1995, 2029, 2070, 2336, 2654
DraI	TTTAAA	1 cut(s)	1813
EaeI	YGGCCR	2 cut(s)	877, 1940
Eam1104I	CTCTTC	2 cut(s)	12, 265
EarI	CTCTTC	2 cut(s)	12, 265
Eco105I	TACGTA	1 cut(s)	858
Eco130I	CCWWGG	1 cut(s)	129
Eco147I	AGGCCT	1 cut(s)	669
Eco24I	GRGCYC	2 cut(s)	1344, 1385
Eco47I	GGWCC	3 cut(s)	1930, 2435, 2613
Eco57I	CTGAAG	5 cut(s)	458, 1222, 2085, 2276, 2567
Eco91I	GGTNACC	1 cut(s)	866
EcoNI	CCTNNNNNAGG	2 cut(s)	674, 2205
EcoO109I	RGGNCCY	3 cut(s)	175, 2022, 2613
EcoO65I	GGTNACC	1 cut(s)	866
EcoRI	GAATTC	3 cut(s)	1389, 2230, 2513
EcoRII	CCWGG	4 cut(s)	168, 1343, 2253, 2437
EcoT14I	CCWWGG	1 cut(s)	129
EcoT22I	ATGCAT	2 cut(s)	627, 2776
EcoT38I	GRGCYC	2 cut(s)	1344, 1385
ErhI	CCWWGG	1 cut(s)	129
FalI	AAGNNNNNCTT	2 cut(s)	636, 668
FaqI	GGGAC	3 cut(s)	761, 1581, 2626
FauI	CCCGC	1 cut(s)	933
FauNDI	CATATG	1 cut(s)	1522
FbaI	TGATCA	1 cut(s)	1839
Fnu4HI	GCNGC	5 cut(s)	123, 291, 492, 2649, 2660
FokI	GGATG	5 cut(s)	146, 486, 936, 1439, 2128
FriOI	GRGCYC	2 cut(s)	1344, 1385
Fsp4HI	GCNGC	5 cut(s)	123, 291, 492, 2649, 2660
FspBI	CTAG	5 cut(s)	213, 863, 1635, 1958, 2792
GluI	GCNGC	5 cut(s)	123, 291, 492, 2649, 2660
GsaI	CCCAGC	1 cut(s)	9
GsuI	CTGGAG	5 cut(s)	191, 478, 776, 807, 1369
HaeIII	GGCC	9 cut(s)	168, 177, 669, 814, 850, 879, 1011, 1942, 2024
HapII	CCGG	2 cut(s)	1616, 2877
HindIII	AAGCTT	5 cut(s)	1271, 1807, 2171, 2489, 2549
HinfI	GANTC	8 cut(s)	496, 760, 950, 1293, 1304, 2072, 2163, 2752
HpaII	CCGG	2 cut(s)	1616, 2877
HphI	GGTGA	9 cut(s)	743, 824, 860, 959, 991, 1334, 1509, 1753, 2321
Hpy166II	GTNNAC	4 cut(s)	855, 2245, 2402, 2738
Hpy8I	GTNNAC	4 cut(s)	855, 2245, 2402, 2738
HpyCH4III	ACNGT	9 cut(s)	400, 775, 1533, 1704, 1789, 1852, 1887, 2428, 2809
HpyCH4IV	ACGT	8 cut(s)	857, 1053, 1161, 1441, 1575, 2088, 2443, 2477
HpyF10VI	GCNNNNNNNGC	8 cut(s)	174, 287, 478, 1270, 1685, 1694, 2557, 2645
HpyF3I	CTNAG	8 cut(s)	360, 485, 1689, 1995, 2029, 2070, 2336, 2654
HpySE526I	ACGT	8 cut(s)	857, 1053, 1161, 1441, 1575, 2088, 2443, 2477
KflI	GGGWCCC	1 cut(s)	2613
Ksp22I	TGATCA	1 cut(s)	1839
LmnI	GCTCC	4 cut(s)	1388, 1616, 2792, 2879
Lsp1109I	GCAGC	5 cut(s)	109, 277, 503, 2660, 2671
LweI	GCATC	7 cut(s)	382, 490, 634, 994, 1415, 2001, 2783
MaeI	CTAG	5 cut(s)	213, 863, 1635, 1958, 2792
MaeII	ACGT	8 cut(s)	857, 1053, 1161, 1441, 1575, 2088, 2443, 2477
MaeIII	GTNAC	8 cut(s)	866, 1062, 1515, 1741, 1852, 2036, 2357, 2841
MfeI	CAATTG	2 cut(s)	526, 1591
MflI	RGATCY	2 cut(s)	893, 1987
MhlI	GDGCHC	3 cut(s)	1344, 1385, 2716
MlsI	TGGCCA	2 cut(s)	879, 1942
MluNI	TGGCCA	2 cut(s)	879, 1942
MlyI	GAGTC	3 cut(s)	1313, 2081, 2172
Mox20I	TGGCCA	2 cut(s)	879, 1942
Mph1103I	ATGCAT	2 cut(s)	627, 2776
MroXI	GAANNNNTTC	1 cut(s)	1965
MscI	TGGCCA	2 cut(s)	879, 1942
MslI	CAYNNNNRTG	4 cut(s)	486, 922, 1605, 2265
Msp20I	TGGCCA	2 cut(s)	879, 1942
MspA1I	CMGCKG	1 cut(s)	389
MspCI	CTTAAG	1 cut(s)	1472
MspI	CCGG	2 cut(s)	1616, 2877
MspR9I	CCNGG	5 cut(s)	170, 1345, 1616, 2255, 2439
MunI	CAATTG	2 cut(s)	526, 1591
MvaI	CCWGG	4 cut(s)	170, 1345, 2255, 2439
MwoI	GCNNNNNNNGC	8 cut(s)	174, 287, 478, 1270, 1685, 1694, 2557, 2645
NciI	CCSGG	1 cut(s)	1616
NcoI	CCATGG	1 cut(s)	129
NdeI	CATATG	1 cut(s)	1522
NlaIV	GGNNCC	6 cut(s)	1384, 1612, 2436, 2614, 2615, 2715
NmuCI	GTSAC	5 cut(s)	866, 1515, 1741, 1852, 2841
NsiI	ATGCAT	2 cut(s)	627, 2776
NspI	RCATGY	2 cut(s)	1024, 1336
NspV	TTCGAA	1 cut(s)	697
PceI	AGGCCT	1 cut(s)	669
PciI	ACATGT	1 cut(s)	1332
PdmI	GAANNNNTTC	1 cut(s)	1965
PfeI	GAWTC	5 cut(s)	496, 760, 950, 1293, 2752
Pfl23II	CGTACG	1 cut(s)	35
PfoI	TCCNGGA	1 cut(s)	2437
PkrI	GCNGC	5 cut(s)	124, 292, 493, 2650, 2661
PleI	GAGTC	3 cut(s)	1312, 2080, 2171
PpsI	GAGTC	3 cut(s)	1312, 2080, 2171
Ppu21I	YACGTR	4 cut(s)	858, 1162, 1442, 2089
PpuMI	RGGWCCY	1 cut(s)	2613
PscI	ACATGT	1 cut(s)	1332
PshBI	ATTAAT	1 cut(s)	321
PsiI	TTATAA	1 cut(s)	1758
Psp5II	RGGWCCY	1 cut(s)	2613
Psp6I	CCWGG	4 cut(s)	168, 1343, 2253, 2437
PspEI	GGTNACC	1 cut(s)	866
PspFI	CCCAGC	1 cut(s)	5
PspGI	CCWGG	4 cut(s)	168, 1343, 2253, 2437
PspLI	CGTACG	1 cut(s)	35
PspN4I	GGNNCC	6 cut(s)	1384, 1612, 2436, 2614, 2615, 2715
PspPI	GGNCC	6 cut(s)	175, 848, 1930, 2022, 2435, 2613
PspPPI	RGGWCCY	1 cut(s)	2613
PstI	CTGCAG	2 cut(s)	295, 2409
PsuI	RGATCY	2 cut(s)	893, 1987
PvuII	CAGCTG	1 cut(s)	389
RseI	CAYNNNNRTG	4 cut(s)	486, 922, 1605, 2265
SatI	GCNGC	5 cut(s)	123, 291, 492, 2649, 2660
Sau96I	GGNCC	6 cut(s)	175, 848, 1930, 2022, 2435, 2613
ScaI	AGTACT	1 cut(s)	2062
SchI	GAGTC	3 cut(s)	1313, 2081, 2172
ScrFI	CCNGG	5 cut(s)	170, 1345, 1616, 2255, 2439
SduI	GDGCHC	3 cut(s)	1344, 1385, 2716
SfaNI	GCATC	7 cut(s)	382, 490, 634, 994, 1415, 2001, 2783
SfcI	CTRYAG	4 cut(s)	291, 396, 1925, 2405
SfiI	GGCCNNNNNGGCC	1 cut(s)	174
SfuI	TTCGAA	1 cut(s)	697
SinI	GGWCC	3 cut(s)	1930, 2435, 2613
SmiMI	CAYNNNNRTG	4 cut(s)	486, 922, 1605, 2265
SmlI	CTYRAG	6 cut(s)	191, 1472, 2017, 2471, 2552, 2618
SmoI	CTYRAG	6 cut(s)	191, 1472, 2017, 2471, 2552, 2618
SnaBI	TACGTA	1 cut(s)	858
SpeI	ACTAGT	1 cut(s)	1634
SseBI	AGGCCT	1 cut(s)	669
SsiI	CCGC	2 cut(s)	940, 1720
SspI	AATATT	2 cut(s)	703, 1171
SspMI	CTAG	5 cut(s)	213, 863, 1635, 1958, 2792
StuI	AGGCCT	1 cut(s)	669
StyD4I	CCNGG	5 cut(s)	168, 1343, 1614, 2253, 2437
StyI	CCWWGG	1 cut(s)	129
TaaI	ACNGT	9 cut(s)	400, 775, 1533, 1704, 1789, 1852, 1887, 2428, 2809
TaiI	ACGT	8 cut(s)	860, 1056, 1164, 1444, 1578, 2091, 2446, 2480
TaqI	TCGA	8 cut(s)	315, 547, 697, 2161, 2218, 2263, 2541, 2817
TaqII	GACCGA	1 cut(s)	723
TatI	WGTACW	5 cut(s)	51, 249, 518, 1704, 2060
TfiI	GAWTC	5 cut(s)	496, 760, 950, 1293, 2752
TscAI	CASTG	6 cut(s)	145, 292, 405, 1536, 2433, 2658
TseFI	GTSAC	5 cut(s)	866, 1515, 1741, 1852, 2841
TseI	GCWGC	5 cut(s)	122, 290, 491, 2648, 2659
Tsp45I	GTSAC	5 cut(s)	866, 1515, 1741, 1852, 2841
TspGWI	ACGGA	4 cut(s)	53, 654, 1475, 2387
TspRI	CASTG	6 cut(s)	145, 292, 405, 1536, 2433, 2658
Vha464I	CTTAAG	1 cut(s)	1472
VpaK11BI	GGWCC	3 cut(s)	1930, 2435, 2613
VspI	ATTAAT	1 cut(s)	321
XagI	CCTNNNNNAGG	2 cut(s)	674, 2205
XceI	RCATGY	2 cut(s)	1024, 1336
XmnI	GAANNNNTTC	1 cut(s)	1965
XspI	CTAG	5 cut(s)	213, 863, 1635, 1958, 2792
ZrmI	AGTACT	1 cut(s)	2062
Zsp2I	ATGCAT	2 cut(s)	627, 2776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.