Rorug04G0008900

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0019173
Physical Location & Seq
Reverse (-)
1443929 .. 1444390
462 bp
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UTR
Exon/CDS
Intron
Rorug04G0008900.1

Sequence Viewer

Length: 462 bp
ATGGAGTTGCTCGAGCCCCTGGGGATGGATAAGGGTAAGGGTACAGAGGTGATGGCTGTTCCCCATCAGAGTTATCCTGCTTCAGGAACTAAGCGTAAAGCTGTGGAGGGACTCGATCGTTTGGGGAAGAGAGGTGTATCTTACACTGAACCTTTACTCCAAGTTGACGATGGGATGCAGCTTGTCCTGCAACACAAAAATGGTCAACTGATTGTCTCCCCCAGGAAGAAGAAATTGGGTAGACCAAAGGGAAGTAAGAACAAACCGAAGTGTGTAAAGGTTCTGGAGAATGAGTTCCAGGGTAGGAAGTCTAAGAAGTGGAAGCTTAGGGAAAGTGATCGCCCTGGTCAAGTGTTGGAGGAAAAAGAGAAAAGCAGTACCTCTGAATATATGGAGCAGCTGCCGACTGATGGTGGCATTTGTGGGGATGTTGGAGCTTCATCCTCAACTAGTAATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RVT

Protein Analysis

153

Amino Acids

16.82

Weight (kDa)

9.49

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24225
fragaria_vesca FvH4_4g06620
prunus_persica Prupe.1G068700_v2.0.a1
pyrus_communis pycom16g20270
rosa_chinensis RchiOBHm_Chr4g0398531
rosa_laevigata RLG00000009347
rosa_rugosa Rorug04G0008900
rosa_samantha Rh4DG078500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 2 cut(s) 43, 379
AfiI CCNNNNNNNGG 3 cut(s) 25, 83, 410
AhlI ACTAGT 1 cut(s) 449
AjnI CCWGG 4 cut(s) 18, 221, 297, 343
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AloI GAACNNNNNNTCC 4 cut(s) 141, 173, 278, 310
AluBI AGCT 5 cut(s) 101, 181, 325, 400, 437
AluI AGCT 5 cut(s) 101, 181, 325, 400, 437
Alw26I GTCTC 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 11
ApeKI GCWGC 3 cut(s) 178, 397, 400
Asp700I GAANNNNTTC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 11
BaeI ACNNNNGTAYC 2 cut(s) 33, 66
BanII GRGCYC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 190, 387, 409
BccI CCATC 5 cut(s) 19, 46, 72, 164, 404
BciT130I CCWGG 4 cut(s) 20, 223, 299, 345
BcoDI GTCTC 1 cut(s) 220
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 1 cut(s) 450
BisI GCNGC 3 cut(s) 179, 398, 401
BlsI GCNGC 3 cut(s) 180, 399, 402
Bme1390I CCNGG 4 cut(s) 20, 223, 299, 345
BmeT110I CYCGRG 1 cut(s) 11
BmrFI CCNGG 4 cut(s) 20, 223, 299, 345
BmsI GCATC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 305
Bpu10I CCTNAGC 1 cut(s) 326
BsaJI CCNNGG 5 cut(s) 18, 19, 221, 298, 343
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 278, 308
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 83, 410
BseBI CCWGG 4 cut(s) 20, 223, 299, 345
BseDI CCNNGG 5 cut(s) 18, 19, 221, 298, 343
BseGI GGATG 4 cut(s) 30, 180, 433, 440
BseLI CCNNNNNNNGG 3 cut(s) 25, 83, 410
BseXI GCAGC 3 cut(s) 190, 387, 409
Bsh1285I CGRYCG 1 cut(s) 118
BsiEI CGRYCG 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 3 cut(s) 25, 83, 410
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 115, 337
BssECI CCNNGG 5 cut(s) 18, 19, 221, 298, 343
BssMI GATC 2 cut(s) 115, 337
Bst2UI CCWGG 4 cut(s) 20, 223, 299, 345
Bst6I CTCTTC 1 cut(s) 122
BstDEI CTNAG 3 cut(s) 90, 312, 326
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 4 cut(s) 30, 180, 433, 440
BstKTI GATC 2 cut(s) 118, 340
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 115, 337
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstNI CCWGG 4 cut(s) 20, 223, 299, 345
BstSCI CCNGG 4 cut(s) 18, 221, 297, 343
BstV1I GCAGC 3 cut(s) 190, 387, 409
BtsCI GGATG 4 cut(s) 30, 180, 433, 440
BtsIMutI CAGTG 1 cut(s) 144
Csp6I GTAC 2 cut(s) 42, 378
CviJI RGCY 7 cut(s) 16, 56, 101, 181, 325, 400, 437
CviKI_1 RGCY 7 cut(s) 16, 56, 101, 181, 325, 400, 437
CviQI GTAC 2 cut(s) 42, 378
DdeI CTNAG 3 cut(s) 90, 312, 326
DpnI GATC 2 cut(s) 117, 339
DpnII GATC 2 cut(s) 115, 337
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco24I GRGCYC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoRII CCWGG 4 cut(s) 18, 221, 297, 343
EcoT38I GRGCYC 1 cut(s) 18
FaiI YATR 2 cut(s) 390, 392
FaqI GGGAC 1 cut(s) 123
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 3 cut(s) 179, 398, 401
FokI GGATG 4 cut(s) 37, 187, 427, 440
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 3 cut(s) 179, 398, 401
FspBI CTAG 1 cut(s) 450
GluI GCNGC 3 cut(s) 179, 398, 401
GsuI CTGGAG 1 cut(s) 305
HincII GTYRAC 2 cut(s) 166, 206
HindII GTYRAC 2 cut(s) 166, 206
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 1 cut(s) 111
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 3 cut(s) 166, 206, 242
Hpy188I TCNGA 2 cut(s) 69, 385
Hpy188III TCNNGA 2 cut(s) 84, 284
Hpy8I GTNNAC 3 cut(s) 166, 206, 242
HpyCH4V TGCA 2 cut(s) 178, 190
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 3 cut(s) 90, 312, 326
Kzo9I GATC 2 cut(s) 115, 337
LmnI GCTCC 2 cut(s) 394, 434
Lsp1109I GCAGC 3 cut(s) 190, 387, 409
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 450
MalI GATC 2 cut(s) 117, 339
MboI GATC 2 cut(s) 115, 337
MboII GAAGA 3 cut(s) 139, 238, 241
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 1 cut(s) 233
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 336, 412
MnlI CCTC 6 cut(s) 40, 100, 125, 352, 391, 454
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 1 cut(s) 460
MslI CAYNNNNRTG 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 400
MspR9I CCNGG 4 cut(s) 20, 223, 299, 345
MvaI CCWGG 4 cut(s) 20, 223, 299, 345
MwoI GCNNNNNNNGC 1 cut(s) 187
NdeII GATC 2 cut(s) 115, 337
PaeR7I CTCGAG 1 cut(s) 11
PasI CCCWGGG 1 cut(s) 19
PdmI GAANNNNTTC 1 cut(s) 293
PkrI GCNGC 3 cut(s) 180, 399, 402
Ple19I CGATCG 1 cut(s) 118
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Psp6I CCWGG 4 cut(s) 18, 221, 297, 343
PspGI CCWGG 4 cut(s) 18, 221, 297, 343
PspXI VCTCGAGB 1 cut(s) 11
PvuI CGATCG 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 2 cut(s) 43, 379
RsaNI GTAC 2 cut(s) 42, 378
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 1 cut(s) 460
SatI GCNGC 3 cut(s) 179, 398, 401
Sau3AI GATC 2 cut(s) 115, 337
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 4 cut(s) 20, 223, 299, 345
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 449
Sse9I AATT 1 cut(s) 233
SspMI CTAG 1 cut(s) 450
StyD4I CCNGG 4 cut(s) 18, 221, 297, 343
TaqI TCGA 2 cut(s) 12, 114
TasI AATT 1 cut(s) 233
Tru1I TTAA 1 cut(s) 460
Tru9I TTAA 1 cut(s) 460
TscAI CASTG 1 cut(s) 151
TseI GCWGC 3 cut(s) 178, 397, 400
TspDTI ATGAA 1 cut(s) 429
TspRI CASTG 1 cut(s) 151
XagI CCTNNNNNAGG 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XhoI CTCGAG 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.