Rorug04G0109200

dCTP pyrophosphatase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017106
Physical Location & Seq
Forward (+)
17081223 .. 17086803
5581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0109200.1

Sequence Viewer

Length: 4662 bp
ATGGCTCCTTCATCTTCATCTGGTGGTTGTTGCAAGTATGATGTCTTCCTCAGTTTTAGAGGAGAAGACACCCGCAAGATCTTCTCCGGCCATCTTTACAAAGCTCTAGAACAGAAAGCAATCTACACCTTCATCGATTCTGAAGAGCTCGAAAAAGGCAACAAAATTTCAGAGCTACTCACATATATTGAGGAATCAAGGATTTCGATTGTCGTTTTATCTCAAAACTATGCCTCTTCCACTTGGTGCTTAAATGAGCTTGTCAAAATCCTGGATTGCATGGTTACGAAGAAGCAGATGGTGCTGCCTATCTTCTACGAAGTGGACCCCTCTGATCTCCGGAAAATCAAAGGAAAATATGCAGAAGCTTTTGCCAAACATGGAGAAAATCCTAACCTTGACAAGGAAGAGGTGAAGAGCTGGAGGTCAGCTGTAAATAGAGTTGCGGATCTATCTGGCTGGGATTCGAGCAAATATGAGGATGACGGCAAGCTTATTGAGGAAATTGTTGCAAATACTCAAAGAAAACTGATCCACACATCGTCCAAAAAAGCCAATGGCTTGATTGGAATGGATTCCCACATTACAGAAATGATGTCAAGATTATGTCTTTGGATGAATGATGTTCGTGTTGTTGGAATATGGGGTATGGGCGGTCTAGGAAAAACAACCATTGCTAGAGCTGTTTTCGATGAAATTGCTTGGCAATTTGAACACTCCTGCTTTCTTGATAATGTCAAAGAAGCTTTTAAAAACAAGCTTGAAGTACAAAAGCAGGAAGAACTTCTATCCAAATTGTTGAAGGAAAAGATGCCAAGTGTAGGTTTGGACAGAGGTAGGAATATGATAATGGAAAGGCTGGGTAGGAAAAAAGTCTTAGTTGTTCTTGATGATGTGGAAACTTATCCCGAAATTGAAGCCTTGCTTGGAAAACTACATGCATTTGGTGAGGGAAGTAGAGTCATTGTGACAACTAGAAATAAGCAATCGCTAAGTGGAGTTAATGGGACATATCAGCCCACGATATTAAGTGATGATGAAGCTCTTGAGCTTTTCATGAAATATGCCTTCAGAACTAATCAACCCAATGGAGACTACGATCGTCTCTCAAGGCGTGCCATAGAATATTCTAAAGGTTTGCCTTTAGCACTTAAAGTCTTGGGGGCTTTTCTTGATAACAAGAGTGTACTTGAGTGGGAAGCCGTGTTAGAGAAATTAAAGAAAATCCCACACATAGAAATTCAGCATGTGCTCAGAACAAGCTTTGATGGACTAGACGATTCAGAGAAGAACATATTTCTAGATATTGCATGTCTCTTTATAGAATGGGGCAAAAATTTTGTTACAGAATTACTAGACAGTTTTGGGTTCTTTCCGCACAATGGATTAAGAGTCCTAATTGATAGAGCTCTCATAACAGTCTCAGATTATAATGATAGGATCTACATGCATGATTTACTACAAGATATGGGTAGAGAAATCGTCCGTCTAGAATCTACTAAAGAACCTTGGAAGCGCAGTAGGTTGTGGAGTTATGAAGATGTTAATCACGTATTGACTCAAAATACGGCTACGGATGCAGTTGAATGCTTAATCCTAGACTTGTCGAACTCAGAAGTCGATCTATGCATAAATACTGGAGCCTATGTTAGAATGACGAAGCTAAGACTACTCGTAATCTATTATTCATTCTACTCCATATTTTATAATTACCAATATAAATCAAGCTATGGAACTCGCAAGTGTGAGGTTAAATTCCCAGTTGATGGCTGTAAACAACTTGCGAAGGGGGAGTTGGAGTTTCTTTCTCATGAGTTGAGGTTTCTCTTATGGCATGGATGCCCTCTAAAGTGTTTTCCATCAACCTTTATCCCAAAGAATCTTGTTAAACTCGACATGCGTGGTAGTCACATTCAACAACTTTGGGAAGGAATCCAGCCGTTGCAAAATTTAAAAGCCATCGTGTTAAATCATTGTCCATACCTTGTCAAAATCCCCGACTTGACTAAAGCAAAAAATCTTGAGAAACTATTTCTTGATGGTTGTTCAAGTTTATTTGAGGTCCATCCATCCATTTCTGGTCTTCAAAACCTTGATTTATTGAAGCTAGCCAACTGCAAAGAACTTAAGATTCTCCCCAGCAGCATTCATATGAAATCTCTAAAGACCCTTGATCTTTTTGGCTGTTGGAATCTTGAAAAGTTTCCAGAAATTTCTCAAGTTATGAATGAGCTAAGCGATCTTCATCTACGTGGGACAGCAATTAAAGAACTGCCCTCATCAATTAATAATCTTACAGGCCTTGTTACTTTGAACCTCAGAGGTTGCAGAGAATTTAAGAGTCTGCCTAGCAGCATCTGTATGAAATCTCTAGAAACCCTTAATCTTTCTGGCTGTTCAAATATGGAGAAGTTTCCAGAGATTTCAGAGGTTATGACGAAGCTAAAATGGCTTCATCTAGATGACACAGCAATAAAAGATATGCCCTCATCAATTAATAACCTTGCAGGGCTTTATACTTTGACCCTCGAAGGTTGCAGAGAACTTAAGAGTCTACCTAGCAGCATTCATTTGAAATCTCTACGGAGCCTTTATCTTTCTGGCTGTTCCAATCTTAAGAAGTTTCCGGAGATTTCAGAAGTTATGAAGGGGGTACGTGAGCTTCATCTAGATGGGACAGCAATTAAAGAATTGCCCTCATCAATTAATAACCTTGTAGGGTTATTTACTTTGAACCTGAAAGGTTGCAGAGAACTTAAGAATCTGCCTAGCAGCATTCATTTGGCATCTCTACAGAGGCTTAATCTTTCTTGCTGTTCCAATCTTGAGAAGTTTCCGGAGATTTCAGAAGTTATGAAGGAGGTACGTGAGCTTCATCTAGATGGGACAGCAATTAAGGAACTGCCCTCATCAATTAAACGGCTTCAGGGTCTTAAATCAGTGAGTATGAGAAACTGCAGAAGCCTGGTCTTTCTTCCAGACAGTGTGTGTAATTTGGCACAGCTTACAAATCTTGATCTGTCTGGGTGCTCAAACTTTTCTAGCTTGCCCGAGAACTTTGGGACTTTAAAATTGTTGGCGGAACTCGAGGTACAAGATAGTGGTGTAAAACAACTTCCGTTGTCTATTTTATGTTTGAGAGCTCTTAAAATATCAATATCATGTAATGGATTCATAGAAATAAAAGCACCCTTTTCAGCATGGTCATCATCAATTTTAAGGCACCTTTCTCAGCATGGTTATCAGCAGACTGGTTATCTGCGACATCTGGATTTAAGTGATTGCAATCTGTTGGAGTTGTCGGATGGCATTACTTGTCTATCTTCATTGGAATCATTGGTTTTGTGCAGAAACAATTTTGAGAGCTTACCTGCAACAATGAATCGACTTGGTCGATTGAGGGATCTCAGATTGGACGATTGCAGTAGACTGAAATCAATACCAGAGCTTTCATCTACTATAAATTACATAGATGCGCATGATTGCATAGCTTTGAACAAAGTTTCAAAGCCGAAGCAACTGTACCATTCGATTTATTTCTTCACATTTTCTAATTGCCTGCAGCTGATACAAACAAATCTATTTAGAGATATTGTGGAAAGGCATTCGCATTTTCAGGATAATTGCCTGCGATCTCTTCGATTTAACATGTCTCTTCCTGGGAGTGAAGTTCCTGATTGGTTCAACCATCACAGCAGCGGATTTTCATTAAGTGTGCAGCTACCACCAAATTGGTTCGACAGTAAGTTTTTGGGATTCGCTATCTGCGCTGTTCGTAAATACCAGGGAGTTCATAATCATCATTTTCCTTTATCTGCTGCTTGCTCTTGTACCTTCAAAGGAAGTCGTGGTGAGTATAGTTTCAGTGTTGATTTACTCGATTCGGTTTTCAGTAATGATAGATTCCTCAAGTCAGATCACATGTTCCTGGGATATGTGCCCTTGTCTGAATTTCCATTAATTGAAGAAGGAAGCCTGGTCAATGAAACCTATTACACTGAGGCCACGTTTGAGATTGCAGTAGACAGAAAATTCAGAATAGAACAACTCCGCATCAGAAGCTGTGGAGTTCATTTTGTTTATGCTAATCAAGATGAAATGCTGAATCTCAGCATGGCCCAGCCCCAAGTTCATGTGGACAGCTGCACGACCGGAGTTTCTTCTATGGACCTGTGTGAAATGAGAAACAGAGAAACAGAGAGGACGGAAATCACTGAAATATTAAGCGAGGTAGAGTCATTGGAGTCAGGGGAAGGGAAAGACACCTGCCACACACTGCAATTGTTTCTGGAACAACAGATAGAGAGAAAAGGAGAGAAAAAGATAGTGGAGATGACAGAGCAGGAGGAGGGGGAGGGTTGCCCGGTGTTAGGATTGGTAATTGTATGCTGCTGCTTGTCATTGTCTGGGTGCCCCATGTGTTCAAGAAACCGAGAAACAGAAAGAGAGGGAGACAGAGTTACCAGAGTCTCTGGAGATGGGGAGATATCGGAGTTGAGCAGCCAGAGTGGTCTGGTGGTGCCAAATTCAGAGATTGAGAGAGAAGAAGAGAAAGAGTTTATGGACGGTTCAGTAATGGGAGACAAGTTATCAGCTGGAAGAGGATTGTTAGGGTCACCATGCAGCTGCTTTGATTGGCTGGCTGCAGTAGTATGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
MazG-like

Protein Analysis

1553

Amino Acids

175.78

Weight (kDa)

6.02

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 186 2.2e-54 TIR domain
TIR_2 PF13676 15 - 116 6.2e-13 TIR domain
NB-ARC PF00931 194 - 360 4.5e-22 NB-ARC domain
WHD_ROQ1 PF23282 428 - 498 4e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 627 - 646 7.1e-06 Leucine Rich Repeat
LRR_14 PF23598 709 - 795 3.8e-08 Leucine-rich repeat region
LRR_13 PF23286 718 - 788 8.7e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 789 - 873 1.3e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 790 - 872 6.2e-08 Leucine-rich repeat region
LRR_13 PF23286 858 - 928 3.9e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 859 - 959 9.7e-08 Leucine-rich repeat region
LRR_13 PF23286 928 - 1013 4.2e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 954 - 1009 7e-06 Leucine-rich repeat region
LRR_13 PF23286 1015 - 1170 2.1e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1086 - 1150 1.5e-06 Leucine-rich repeat region
C-JID PF20160 1219 - 1365 4.5e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017106)

Species Orthologous Gene IDs
malus_domestica MD04G1025100.v1.1 MD04G1025300.v1.1
pyrus_communis pycom04g02150
rosa_chinensis RchiOBHm_Chr4g0411401
rosa_laevigata RLG00000008359
rosa_multiflora Rmu_co8074190.1_g000001 Rmu_sc0001982.1_g000008
rosa_roxburghii Rroxscaffold_5G00355990
rosa_rugosa Rorug04G0109200
rosa_samantha Rh4AG167600 Rh4CG179500 Rh4DG162400
rosa_wichuraiana Rw4G013860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1431, 1707
AarI CACCTGC 1 cut(s) 4274
Acc16I TGCGCA 1 cut(s) 3477
Acc36I ACCTGC 2 cut(s) 3379, 4274
AccB1I GGYRCC 3 cut(s) 3222, 4410, 4519
AccB7I CCANNNNNTGG 1 cut(s) 1766
AccI GTMKAC 3 cut(s) 2554, 3427, 4023
AccIII TCCGGA 3 cut(s) 339, 2626, 2836
AciI CCGC 7 cut(s) 73, 446, 654, 1376, 3080, 3699, 4051
AclWI GGATC 4 cut(s) 456, 526, 1448, 3411
AcoI YGGCCR 1 cut(s) 88
AcuI CTGAAG 3 cut(s) 162, 1054, 2909
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 7 cut(s) 768, 1188, 2655, 2865, 3093, 3524, 3832
AfiI CCNNNNNNNGG 5 cut(s) 403, 821, 1382, 1766, 4370
AflII CTTAAG 4 cut(s) 2126, 2546, 2615, 2756
AflIII ACRYGT 2 cut(s) 3648, 3921
AjnI CCWGG 6 cut(s) 270, 2964, 3658, 3783, 3927, 3975
AjuI GAANNNNNNNTTGG 4 cut(s) 909, 941, 1778, 1810
Alw21I GWGCWC 5 cut(s) 150, 1254, 1411, 3032, 3145
Alw26I GTCTC 8 cut(s) 1086, 1109, 1319, 1426, 3657, 4446, 4474, 4575
AlwI GGATC 4 cut(s) 456, 526, 1448, 3411
AlwNI CAGNNNCTG 2 cut(s) 2358, 4062
Ama87I CYCGRG 2 cut(s) 3050, 3086
Aor13HI TCCGGA 3 cut(s) 339, 2626, 2836
AoxI GGCC 4 cut(s) 88, 2299, 4002, 4116
AseI ATTAAT 4 cut(s) 2286, 2496, 2706, 3959
Asp700I GAANNNNTTC 3 cut(s) 574, 783, 2202
AspLEI GCGC 3 cut(s) 1518, 3478, 3770
AspS9I GGNCC 4 cut(s) 325, 2062, 4117, 4168
AsuC2I CCSGG 1 cut(s) 4364
AsuHPI GGTGA 4 cut(s) 424, 959, 3863, 4608
AsuNHI GCTAGC 1 cut(s) 2107
AvaI CYCGRG 2 cut(s) 3050, 3086
AvaII GGWCC 3 cut(s) 325, 2062, 4168
BaeGI GKGCMC 2 cut(s) 3942, 4415
BanI GGYRCC 3 cut(s) 3222, 4410, 4519
BanII GRGCYC 3 cut(s) 150, 1411, 3145
BbsI GAAGAC 3 cut(s) 37, 72, 2075
Bbv12I GWGCWC 5 cut(s) 150, 1254, 1411, 3032, 3145
BceAI ACGGC 5 cut(s) 502, 1187, 1584, 1924, 2936
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 6 cut(s) 272, 2966, 3660, 3785, 3929, 3977
BcnI CCSGG 1 cut(s) 4364
BcoDI GTCTC 8 cut(s) 1086, 1109, 1319, 1426, 3657, 4446, 4474, 4575
BfmI CTRYAG 4 cut(s) 2792, 2956, 3560, 4644
BfrI CTTAAG 4 cut(s) 2126, 2546, 2615, 2756
BfuAI ACCTGC 2 cut(s) 3379, 4274
BglII AGATCT 1 cut(s) 78
BlpI GCTNAGC 1 cut(s) 2234
Bme1390I CCNGG 7 cut(s) 272, 2966, 3660, 3785, 3929, 3977, 4364
Bme18I GGWCC 3 cut(s) 325, 2062, 4168
BmeT110I CYCGRG 2 cut(s) 3050, 3086
BmgT120I GGNCC 4 cut(s) 325, 2062, 4117, 4168
BmiI GGNNCC 7 cut(s) 6, 327, 1642, 2588, 3224, 4412, 4521
BmrFI CCNGG 7 cut(s) 272, 2966, 3660, 3785, 3929, 3977, 4364
BmrI ACTGGG 1 cut(s) 1754
BmsI GCATC 7 cut(s) 801, 1567, 1829, 2364, 2795, 3463, 4062
BmtI GCTAGC 1 cut(s) 2111
BmuI ACTGGG 1 cut(s) 1754
BpiI GAAGAC 3 cut(s) 37, 72, 2075
BpmI CTGGAG 3 cut(s) 442, 1659, 4494
Bpu1102I GCTNAGC 1 cut(s) 2234
BpuEI CTTGAG 7 cut(s) 1067, 1093, 1211, 2042, 2202, 2846, 3893
BpuMI CCSGG 1 cut(s) 4364
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 4 cut(s) 1552, 2252, 2657, 2867
BsaBI GATNNNNATC 3 cut(s) 1545, 2244, 3285
BsaJI CCNNGG 4 cut(s) 1508, 3659, 3784, 3928
BsaWI WCCGGW 4 cut(s) 339, 2626, 2836, 4151
BsaXI ACNNNNNCTCC 6 cut(s) 1632, 1662, 1784, 1814, 4443, 4473
Bsc4I CCNNNNNNNGG 5 cut(s) 403, 821, 1382, 1766, 4370
Bse1I ACTGG 3 cut(s) 1642, 1760, 3256
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 3 cut(s) 1545, 2244, 3285
BseAI TCCGGA 3 cut(s) 339, 2626, 2836
BseBI CCWGG 6 cut(s) 272, 2966, 3660, 3785, 3929, 3977
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 4 cut(s) 1508, 3659, 3784, 3928
BseGI GGATG 7 cut(s) 487, 621, 1582, 1844, 2065, 2069, 3310
BseJI GATNNNNATC 3 cut(s) 1545, 2244, 3285
BseLI CCNNNNNNNGG 5 cut(s) 403, 821, 1382, 1766, 4370
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 9 cut(s) 64, 1267, 1437, 1626, 2332, 3245, 3421, 3990, 4123
BseNI ACTGG 3 cut(s) 1642, 1760, 3256
BseRI GAGGAG 2 cut(s) 75, 4361
BseSI GKGCMC 2 cut(s) 3942, 4415
BseYI CCCAGC 4 cut(s) 459, 859, 2138, 4119
BsgI GTGCAG 3 cut(s) 3367, 3737, 4129
Bsh1285I CGRYCG 2 cut(s) 1102, 4152
BshFI GGCC 4 cut(s) 90, 2301, 4004, 4118
BshNI GGYRCC 3 cut(s) 3222, 4410, 4519
BshVI ATCGAT 1 cut(s) 135
BsiEI CGRYCG 2 cut(s) 1102, 4152
BsiHKAI GWGCWC 5 cut(s) 150, 1254, 1411, 3032, 3145
BsiHKCI CYCGRG 2 cut(s) 3050, 3086
BsiSI CCGG 6 cut(s) 87, 340, 2627, 2837, 4152, 4364
BslFI GGGAC 5 cut(s) 1021, 2269, 2689, 2899, 3076
BslI CCNNNNNNNGG 5 cut(s) 403, 821, 1382, 1766, 4370
BsmAI GTCTC 8 cut(s) 1086, 1109, 1319, 1426, 3657, 4446, 4474, 4575
BsmBI CGTCTC 1 cut(s) 1109
BsmFI GGGAC 5 cut(s) 1021, 2269, 2689, 2899, 3076
BsmI GAATGC 5 cut(s) 1592, 2145, 2565, 2775, 3604
BsnI GGCC 4 cut(s) 90, 2301, 4004, 4118
BsoBI CYCGRG 2 cut(s) 3050, 3086
Bsp1286I GDGCHC 7 cut(s) 150, 1254, 1411, 3032, 3145, 3942, 4415
Bsp13I TCCGGA 3 cut(s) 339, 2626, 2836
Bsp1720I GCTNAGC 1 cut(s) 2234
BspACI CCGC 7 cut(s) 73, 446, 654, 1376, 3080, 3699, 4051
BspANI GGCC 4 cut(s) 90, 2301, 4004, 4118
BspCNI CTCAG 9 cut(s) 63, 1266, 1436, 1625, 2331, 3244, 3420, 3991, 4122
BspDI ATCGAT 1 cut(s) 135
BspEI TCCGGA 3 cut(s) 339, 2626, 2836
BspHI TCATGA 2 cut(s) 1056, 1810
BspLI GGNNCC 7 cut(s) 6, 327, 1642, 2588, 3224, 4412, 4521
BspMAI CTGCAG 3 cut(s) 2960, 3564, 4648
BspMI ACCTGC 2 cut(s) 3379, 4274
BspOI GCTAGC 1 cut(s) 2111
BspPI GGATC 4 cut(s) 456, 526, 1448, 3411
BspQI GCTCTTC 2 cut(s) 138, 410
BspT107I GGYRCC 3 cut(s) 3222, 4410, 4519
BspTI CTTAAG 4 cut(s) 2126, 2546, 2615, 2756
BsrDI GCAATG 1 cut(s) 672
BsrI ACTGG 3 cut(s) 1642, 1760, 3256
BssECI CCNNGG 4 cut(s) 1508, 3659, 3784, 3928
BssT1I CCWWGG 1 cut(s) 1508
Bst2UI CCWGG 6 cut(s) 272, 2966, 3660, 3785, 3929, 3977
Bst4CI ACNGT 6 cut(s) 1361, 1420, 2984, 3522, 3743, 4568
Bst6I CTCTTC 8 cut(s) 138, 241, 402, 410, 3642, 3660, 4542, 4594
BstAFI CTTAAG 4 cut(s) 2126, 2546, 2615, 2756
BstAPI GCANNNNNTGC 1 cut(s) 301
BstBAI YACGTR 4 cut(s) 1552, 2252, 2657, 2867
BstC8I GCNNGC 8 cut(s) 491, 1116, 2109, 3047, 3560, 3629, 3823, 4641
BstEII GGTNACC 1 cut(s) 4614
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 7 cut(s) 487, 621, 1582, 1844, 2065, 2069, 3310
BstHHI GCGC 3 cut(s) 1518, 3478, 3770
BstMAI GTCTC 8 cut(s) 1086, 1109, 1319, 1426, 3657, 4446, 4474, 4575
BstMCI CGRYCG 2 cut(s) 1102, 4152
BstMWI GCNNNNNNNGC 5 cut(s) 301, 1577, 2449, 3767, 4059
BstNI CCWGG 6 cut(s) 272, 2966, 3660, 3785, 3929, 3977
BstNSI RCATGY 7 cut(s) 941, 1250, 1314, 1450, 1900, 3652, 3925
BstPI GGTNACC 1 cut(s) 4614
BstSCI CCNGG 7 cut(s) 270, 2964, 3658, 3783, 3927, 3975, 4362
BstSFI CTRYAG 4 cut(s) 2792, 2956, 3560, 4644
BstSLI GKGCMC 2 cut(s) 3942, 4415
BstV2I GAAGAC 3 cut(s) 37, 72, 2075
BstX2I RGATCY 4 cut(s) 78, 448, 1440, 3403
BstXI CCANNNNNNTGG 1 cut(s) 3732
BstYI RGATCY 4 cut(s) 78, 448, 1440, 3403
Bsu15I ATCGAT 1 cut(s) 135
BsuRI GGCC 4 cut(s) 90, 2301, 4004, 4118
BsuTUI ATCGAT 1 cut(s) 135
BtsCI GGATG 7 cut(s) 487, 621, 1582, 1844, 2065, 2069, 3310
BtsI GCAGTG 1 cut(s) 4274
BtsIMutI CAGTG 6 cut(s) 2946, 2989, 3871, 3996, 4212, 4274
BveI ACCTGC 2 cut(s) 3379, 4274
Cac8I GCNNGC 8 cut(s) 491, 1116, 2109, 3047, 3560, 3629, 3823, 4641
CaiI CAGNNNCTG 2 cut(s) 2358, 4062
CciI TCATGA 2 cut(s) 1056, 1810
CfoI GCGC 3 cut(s) 1518, 3478, 3770
Cfr13I GGNCC 4 cut(s) 325, 2062, 4117, 4168
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 7 cut(s) 767, 1187, 2654, 2864, 3092, 3523, 3831
CviQI GTAC 7 cut(s) 767, 1187, 2654, 2864, 3092, 3523, 3831
DraI TTTAAA 3 cut(s) 751, 1953, 3069
DraIII CACNNNGTG 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 8 cut(s) 138, 241, 402, 410, 3642, 3660, 4542, 4594
EarI CTCTTC 8 cut(s) 138, 241, 402, 410, 3642, 3660, 4542, 4594
EciI GGCGGA 1 cut(s) 3095
Ecl136II GAGCTC 3 cut(s) 148, 1409, 3143
Eco130I CCWWGG 1 cut(s) 1508
Eco147I AGGCCT 1 cut(s) 2301
Eco24I GRGCYC 3 cut(s) 150, 1411, 3145
Eco32I GATATC 1 cut(s) 4488
Eco47I GGWCC 3 cut(s) 325, 2062, 4168
Eco53kI GAGCTC 3 cut(s) 148, 1409, 3143
Eco57I CTGAAG 3 cut(s) 162, 1054, 2909
Eco88I CYCGRG 2 cut(s) 3050, 3086
Eco91I GGTNACC 1 cut(s) 4614
EcoICRI GAGCTC 3 cut(s) 148, 1409, 3143
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 4614
EcoRII CCWGG 6 cut(s) 270, 2964, 3658, 3783, 3927, 3975
EcoRV GATATC 1 cut(s) 4488
EcoT14I CCWWGG 1 cut(s) 1508
EcoT22I ATGCAT 3 cut(s) 943, 1452, 1631
EcoT38I GRGCYC 3 cut(s) 150, 1411, 3145
ErhI CCWWGG 1 cut(s) 1508
Esp3I CGTCTC 1 cut(s) 1109
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FaqI GGGAC 5 cut(s) 1021, 2269, 2689, 2899, 3076
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 1 cut(s) 2151
FblI GTMKAC 3 cut(s) 2554, 3427, 4023
FokI GGATG 7 cut(s) 494, 628, 1589, 1851, 2052, 2056, 3317
FriOI GRGCYC 3 cut(s) 150, 1411, 3145
FspAI RTGCGCAY 1 cut(s) 3477
FspI TGCGCA 1 cut(s) 3477
GlaI GCGC 3 cut(s) 1517, 3477, 3769
GsaI CCCAGC 4 cut(s) 463, 863, 2142, 4123
GsuI CTGGAG 3 cut(s) 442, 1659, 4494
HaeIII GGCC 4 cut(s) 90, 2301, 4004, 4118
HapII CCGG 6 cut(s) 87, 340, 2627, 2837, 4152, 4364
HhaI GCGC 3 cut(s) 1518, 3478, 3770
Hin6I GCGC 3 cut(s) 1516, 3476, 3768
HinP1I GCGC 3 cut(s) 1516, 3476, 3768
HindIII AAGCTT 5 cut(s) 366, 491, 744, 758, 1261
HpaII CCGG 6 cut(s) 87, 340, 2627, 2837, 4152, 4364
HphI GGTGA 4 cut(s) 424, 959, 3863, 4608
Hpy166II GTNNAC 7 cut(s) 325, 1187, 1775, 2555, 3428, 4024, 4138
Hpy8I GTNNAC 7 cut(s) 325, 1187, 1775, 2555, 3428, 4024, 4138
HpyCH4III ACNGT 6 cut(s) 1361, 1420, 2984, 3522, 3743, 4568
HpyCH4IV ACGT 5 cut(s) 1551, 2251, 2656, 2866, 4007
HpyF10VI GCNNNNNNNGC 5 cut(s) 301, 1577, 2449, 3767, 4059
HpySE526I ACGT 5 cut(s) 1551, 2251, 2656, 2866, 4007
HspAI GCGC 3 cut(s) 1516, 3476, 3768
Kpn2I TCCGGA 3 cut(s) 339, 2626, 2836
LguI GCTCTTC 2 cut(s) 138, 410
LmnI GCTCC 3 cut(s) 10, 1640, 2586
LweI GCATC 7 cut(s) 801, 1567, 1829, 2364, 2795, 3463, 4062
MaeII ACGT 5 cut(s) 1551, 2251, 2656, 2866, 4007
MaeIII GTNAC 7 cut(s) 283, 967, 1342, 1907, 2305, 4459, 4614
MfeI CAATTG 1 cut(s) 4280
MflI RGATCY 4 cut(s) 78, 448, 1440, 3403
MhlI GDGCHC 7 cut(s) 150, 1254, 1411, 3032, 3145, 3942, 4415
MlyI GAGTC 8 cut(s) 969, 1401, 1552, 2350, 2560, 4244, 4253, 4476
MmeI TCCRAC 5 cut(s) 616, 1776, 2168, 3273, 3282
Mph1103I ATGCAT 3 cut(s) 943, 1452, 1631
MroI TCCGGA 3 cut(s) 339, 2626, 2836
MroXI GAANNNNTTC 3 cut(s) 574, 783, 2202
MslI CAYNNNNRTG 6 cut(s) 2150, 2250, 2360, 2460, 2670, 2880
MspA1I CMGCKG 6 cut(s) 431, 3565, 3699, 4143, 4595, 4626
MspCI CTTAAG 4 cut(s) 2126, 2546, 2615, 2756
MspI CCGG 6 cut(s) 87, 340, 2627, 2837, 4152, 4364
MspR9I CCNGG 7 cut(s) 272, 2966, 3660, 3785, 3929, 3977, 4364
MunI CAATTG 1 cut(s) 4280
Mva1269I GAATGC 5 cut(s) 1592, 2145, 2565, 2775, 3604
MvaI CCWGG 6 cut(s) 272, 2966, 3660, 3785, 3929, 3977
MwoI GCNNNNNNNGC 5 cut(s) 301, 1577, 2449, 3767, 4059
NciI CCSGG 1 cut(s) 4364
NdeI CATATG 1 cut(s) 2151
NheI GCTAGC 1 cut(s) 2107
NlaIV GGNNCC 7 cut(s) 6, 327, 1642, 2588, 3224, 4412, 4521
NmuCI GTSAC 3 cut(s) 967, 1907, 4614
NsbI TGCGCA 1 cut(s) 3477
NsiI ATGCAT 3 cut(s) 943, 1452, 1631
NspI RCATGY 7 cut(s) 941, 1250, 1314, 1450, 1900, 3652, 3925
PaeR7I CTCGAG 1 cut(s) 3086
PagI TCATGA 2 cut(s) 1056, 1810
PaqCI CACCTGC 1 cut(s) 4274
PceI AGGCCT 1 cut(s) 2301
PciI ACATGT 2 cut(s) 3648, 3921
PciSI GCTCTTC 2 cut(s) 138, 410
PcsI WCGNNNNNNNCGW 1 cut(s) 3391
PctI GAATGC 5 cut(s) 1592, 2145, 2565, 2775, 3604
PdmI GAANNNNTTC 3 cut(s) 574, 783, 2202
PflFI GACNNNGTC 1 cut(s) 3390
PflMI CCANNNNNTGG 1 cut(s) 1766
PfoI TCCNGGA 1 cut(s) 270
Ple19I CGATCG 1 cut(s) 1102
PleI GAGTC 8 cut(s) 968, 1400, 1552, 2349, 2559, 4243, 4252, 4475
PpsI GAGTC 8 cut(s) 968, 1400, 1552, 2349, 2559, 4243, 4252, 4475
Ppu21I YACGTR 4 cut(s) 1552, 2252, 2657, 2867
PscI ACATGT 2 cut(s) 3648, 3921
PshBI ATTAAT 4 cut(s) 2286, 2496, 2706, 3959
PsiI TTATAA 2 cut(s) 1431, 1707
Psp124BI GAGCTC 3 cut(s) 150, 1411, 3145
Psp6I CCWGG 6 cut(s) 270, 2964, 3658, 3783, 3927, 3975
PspEI GGTNACC 1 cut(s) 4614
PspFI CCCAGC 4 cut(s) 459, 859, 2138, 4119
PspGI CCWGG 6 cut(s) 270, 2964, 3658, 3783, 3927, 3975
PspN4I GGNNCC 7 cut(s) 6, 327, 1642, 2588, 3224, 4412, 4521
PspPI GGNCC 4 cut(s) 325, 2062, 4117, 4168
PspXI VCTCGAGB 1 cut(s) 3086
PsrI GAACNNNNNNTAC 2 cut(s) 3075, 3107
PstI CTGCAG 3 cut(s) 2960, 3564, 4648
PstNI CAGNNNCTG 2 cut(s) 2358, 4062
PsuI RGATCY 4 cut(s) 78, 448, 1440, 3403
PsyI GACNNNGTC 1 cut(s) 3390
PvuI CGATCG 1 cut(s) 1102
PvuII CAGCTG 5 cut(s) 431, 3565, 4143, 4595, 4626
RsaI GTAC 7 cut(s) 768, 1188, 2655, 2865, 3093, 3524, 3832
RsaNI GTAC 7 cut(s) 767, 1187, 2654, 2864, 3092, 3523, 3831
RseI CAYNNNNRTG 6 cut(s) 2150, 2250, 2360, 2460, 2670, 2880
SacI GAGCTC 3 cut(s) 150, 1411, 3145
SapI GCTCTTC 2 cut(s) 138, 410
Sau96I GGNCC 4 cut(s) 325, 2062, 4117, 4168
SchI GAGTC 8 cut(s) 969, 1401, 1552, 2350, 2560, 4244, 4253, 4476
ScrFI CCNGG 7 cut(s) 272, 2966, 3660, 3785, 3929, 3977, 4364
SduI GDGCHC 7 cut(s) 150, 1254, 1411, 3032, 3145, 3942, 4415
SfaNI GCATC 7 cut(s) 801, 1567, 1829, 2364, 2795, 3463, 4062
SfcI CTRYAG 4 cut(s) 2792, 2956, 3560, 4644
Sfr274I CTCGAG 1 cut(s) 3086
SinI GGWCC 3 cut(s) 325, 2062, 4168
SlaI CTCGAG 1 cut(s) 3086
SmiMI CAYNNNNRTG 6 cut(s) 2150, 2250, 2360, 2460, 2670, 2880
SseBI AGGCCT 1 cut(s) 2301
SsiI CCGC 7 cut(s) 73, 446, 654, 1376, 3080, 3699, 4051
SspI AATATT 2 cut(s) 1127, 4221
SstI GAGCTC 3 cut(s) 150, 1411, 3145
StuI AGGCCT 1 cut(s) 2301
StyD4I CCNGG 7 cut(s) 270, 2964, 3658, 3783, 3927, 3975, 4362
StyI CCWWGG 1 cut(s) 1508
TaaI ACNGT 6 cut(s) 1361, 1420, 2984, 3522, 3743, 4568
TaiI ACGT 5 cut(s) 1554, 2254, 2659, 2869, 4010
TatI WGTACW 2 cut(s) 766, 1186
TscAI CASTG 6 cut(s) 2946, 2989, 3871, 4003, 4219, 4281
TseFI GTSAC 3 cut(s) 967, 1907, 4614
Tsp45I GTSAC 3 cut(s) 967, 1907, 4614
TspGWI ACGGA 5 cut(s) 1475, 1589, 2599, 3108, 4220
TspRI CASTG 6 cut(s) 2946, 2989, 3871, 4003, 4219, 4281
Tth111I GACNNNGTC 1 cut(s) 3390
Van91I CCANNNNNTGG 1 cut(s) 1766
Vha464I CTTAAG 4 cut(s) 2126, 2546, 2615, 2756
VpaK11BI GGWCC 3 cut(s) 325, 2062, 4168
VspI ATTAAT 4 cut(s) 2286, 2496, 2706, 3959
XagI CCTNNNNNAGG 1 cut(s) 401
XbaI TCTAGA 7 cut(s) 106, 1300, 1489, 2371, 2458, 2668, 2878
XceI RCATGY 7 cut(s) 941, 1250, 1314, 1450, 1900, 3652, 3925
XhoI CTCGAG 1 cut(s) 3086
XmiI GTMKAC 3 cut(s) 2554, 3427, 4023
XmnI GAANNNNTTC 3 cut(s) 574, 783, 2202
Zsp2I ATGCAT 3 cut(s) 943, 1452, 1631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.