Rorug04G0152400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009100
Physical Location & Seq
Forward (+)
26721275 .. 26722058
784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0152400.1

Sequence Viewer

Length: 684 bp
ATGGAGGATAGCGGCTGCATCTCGGACGGGGAAGAGCGCTCTGGATCTCTTGTTCTCAGATTATGGGTGAGACCGGACATGGAGCAGTGGCGGAGGCTTCAGGCGTCACGACCGGTTCTCCGTGCTTGGCGGTGGCTGGGTCTCCAATCTCGTGGCAGAGATGAGCCCATAGTGGGTCTGCGTCTTTCTGAGATCTGGGTTCTGACGTCGGCCAGGCTTTGGGACGGTCGGCTTTGTGGGGTGGCGGTGTTGTGGCGTTGGGATGCGTTGCGGCGGCAGTCCAGTGCAAGGCAACAGGACGGTGGAGCTCGCTGGCAGCTTGCTAGGTGGACGGCGACGCTAGTTGTTTGGGGTGAGAGGTGGTTGGGCCGGACCTTTGTGTCTGGGTCATGCCACTGGTTGGACCGGGCCTTTATGTCAAGGCCATGCTGCTTGGGCGTTTCTTTGTTTTTATTTGTTGTTGTTGTCTTCAGCGTTTTAGTTACTTGCAGTGTTTCTGTTTTTGCTTTGTCGGAAATAAGCTCCAACCGTGTTCTGGTTGGAGCCAGTGGCCTGTGTGCCAATCTTTGCACCTTGTGTGCCAGTTTTGCTCTAGGAGAGCTGTGCGTATGGTTGTTATGCTTTGCTGTAATCGGGTTGCAGATCAAGTTATCTTTCCGTTGTGTCACCGCTGCGTTAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.53

Weight (kDa)

9.39

Isoelectric Point (pI)

65.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 379
AatII GACGTC 1 cut(s) 209
AccB7I CCANNNNNTGG 2 cut(s) 219, 400
AciI CCGC 7 cut(s) 12, 91, 130, 245, 271, 274, 669
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 1 cut(s) 210
AcuI CTGAAG 2 cut(s) 83, 454
AcyI GRCGYC 2 cut(s) 104, 206
AdeI CACNNNGTG 1 cut(s) 576
AfeI AGCGCT 1 cut(s) 38
AfiI CCNNNNNNNGG 5 cut(s) 173, 219, 288, 400, 535
AgeI ACCGGT 1 cut(s) 112
AjnI CCWGG 1 cut(s) 212
AloI GAACNNNNNNTCC 2 cut(s) 36, 68
AluBI AGCT 4 cut(s) 308, 319, 522, 601
AluI AGCT 4 cut(s) 308, 319, 522, 601
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 2 cut(s) 64, 146
AlwI GGATC 1 cut(s) 52
Aor51HI AGCGCT 1 cut(s) 38
AoxI GGCC 5 cut(s) 210, 367, 408, 422, 550
ApeKI GCWGC 4 cut(s) 15, 316, 429, 671
AsiGI ACCGGT 1 cut(s) 112
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 4 cut(s) 367, 372, 403, 408
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 3 cut(s) 79, 365, 658
AvaII GGWCC 2 cut(s) 372, 403
BanII GRGCYC 2 cut(s) 168, 310
BauI CACGAG 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 4 cut(s) 2, 328, 416, 658
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 214
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 64, 146
BfaI CTAG 3 cut(s) 324, 341, 593
BfoI RGCGCY 1 cut(s) 40
BglII AGATCT 1 cut(s) 192
BisI GCNGC 7 cut(s) 13, 16, 272, 275, 317, 430, 672
BlsI GCNGC 7 cut(s) 14, 17, 273, 276, 318, 431, 673
Bme1390I CCNGG 2 cut(s) 214, 407
Bme18I GGWCC 2 cut(s) 372, 403
BmgT120I GGNCC 4 cut(s) 367, 372, 403, 408
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 214, 407
BmsI GCATC 2 cut(s) 27, 253
BpiI GAAGAC 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 407
BsaHI GRCGYC 2 cut(s) 104, 206
BsaI GGTCTC 2 cut(s) 64, 146
BsaWI WCCGGW 2 cut(s) 73, 112
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 219, 288, 400, 535
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 4 cut(s) 282, 401, 546, 582
BseBI CCWGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 5 cut(s) 173, 219, 288, 400, 535
BseMII CTCAG 2 cut(s) 70, 180
BseNI ACTGG 4 cut(s) 282, 401, 546, 582
BseXI GCAGC 4 cut(s) 2, 328, 416, 658
BseYI CCCAGC 1 cut(s) 136
Bsh1285I CGRYCG 2 cut(s) 113, 229
BshFI GGCC 5 cut(s) 212, 369, 410, 424, 552
BshTI ACCGGT 1 cut(s) 112
BsiEI CGRYCG 2 cut(s) 113, 229
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 4 cut(s) 74, 113, 370, 406
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 5 cut(s) 173, 219, 288, 400, 535
BsmAI GTCTC 2 cut(s) 64, 146
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 5 cut(s) 212, 369, 410, 424, 552
Bso31I GGTCTC 2 cut(s) 64, 146
Bsp1286I GDGCHC 2 cut(s) 168, 310
Bsp143I GATC 3 cut(s) 44, 192, 642
BspACI CCGC 7 cut(s) 12, 91, 130, 245, 271, 274, 669
BspANI GGCC 5 cut(s) 212, 369, 410, 424, 552
BspCNI CTCAG 2 cut(s) 69, 181
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 1 cut(s) 52
BspQI GCTCTTC 1 cut(s) 27
BspTNI GGTCTC 2 cut(s) 64, 146
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 4 cut(s) 282, 401, 546, 582
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 3 cut(s) 44, 192, 642
BssNI GRCGYC 2 cut(s) 104, 206
BssSI CACGAG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 214
Bst4CI ACNGT 3 cut(s) 227, 302, 530
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 2 cut(s) 104, 206
BstC8I GCNNGC 3 cut(s) 310, 314, 321
BstDEI CTNAG 2 cut(s) 56, 189
BstF5I GGATG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 47, 195, 645
BstMAI GTCTC 2 cut(s) 64, 146
BstMBI GATC 3 cut(s) 44, 192, 642
BstMCI CGRYCG 2 cut(s) 113, 229
BstMWI GCNNNNNNNGC 2 cut(s) 435, 587
BstNI CCWGG 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 212, 405
BstV1I GCAGC 4 cut(s) 2, 328, 416, 658
BstV2I GAAGAC 1 cut(s) 460
BstX2I RGATCY 2 cut(s) 44, 192
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 2 cut(s) 44, 192
BsuRI GGCC 5 cut(s) 212, 369, 410, 424, 552
BtsCI GGATG 1 cut(s) 268
BtsI GCAGTG 2 cut(s) 92, 496
BtsIMutI CAGTG 5 cut(s) 92, 289, 394, 496, 553
Cac8I GCNNGC 3 cut(s) 310, 314, 321
CfoI GCGC 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 367, 372, 403, 408
CseI GACGC 3 cut(s) 93, 170, 346
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 3 cut(s) 79, 390, 426
DdeI CTNAG 2 cut(s) 56, 189
DpnI GATC 3 cut(s) 46, 194, 644
DpnII GATC 3 cut(s) 44, 192, 642
DraIII CACNNNGTG 1 cut(s) 576
DrdI GACNNNNNNGTC 1 cut(s) 379
DseDI GACNNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 308
Eco24I GRGCYC 2 cut(s) 168, 310
Eco31I GGTCTC 2 cut(s) 64, 146
Eco47I GGWCC 2 cut(s) 372, 403
Eco47III AGCGCT 1 cut(s) 38
Eco53kI GAGCTC 1 cut(s) 308
Eco57I CTGAAG 2 cut(s) 83, 454
EcoICRI GAGCTC 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 212
EcoT38I GRGCYC 2 cut(s) 168, 310
FaeI CATG 3 cut(s) 82, 393, 429
FaiI YATR 8 cut(s) 64, 80, 170, 391, 416, 427, 610, 619
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 78, 389, 425
Fnu4HI GCNGC 7 cut(s) 13, 16, 272, 275, 317, 430, 672
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 2 cut(s) 168, 310
Fsp4HI GCNGC 7 cut(s) 13, 16, 272, 275, 317, 430, 672
FspBI CTAG 3 cut(s) 324, 341, 593
GlaI GCGC 1 cut(s) 38
GluI GCNGC 7 cut(s) 13, 16, 272, 275, 317, 430, 672
GsaI CCCAGC 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 5 cut(s) 212, 369, 410, 424, 552
HapII CCGG 4 cut(s) 74, 113, 370, 406
HgaI GACGC 3 cut(s) 93, 170, 346
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 2 cut(s) 104, 206
Hin1II CATG 3 cut(s) 82, 393, 429
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HpaII CCGG 4 cut(s) 74, 113, 370, 406
HphI GGTGA 3 cut(s) 79, 365, 658
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 5 cut(s) 25, 59, 190, 204, 514
Hpy188III TCNNGA 2 cut(s) 42, 108
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 2 cut(s) 211, 340
HpyCH4III ACNGT 3 cut(s) 227, 302, 530
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 5 cut(s) 18, 287, 489, 570, 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 435, 587
HpyF3I CTNAG 2 cut(s) 56, 189
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 2 cut(s) 104, 206
Hsp92II CATG 3 cut(s) 82, 393, 429
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 44, 192, 642
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 4 cut(s) 82, 305, 527, 542
Lsp1109I GCAGC 4 cut(s) 2, 328, 416, 658
LweI GCATC 2 cut(s) 27, 253
MaeI CTAG 3 cut(s) 324, 341, 593
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 3 cut(s) 105, 481, 664
MalI GATC 3 cut(s) 46, 194, 644
MboI GATC 3 cut(s) 44, 192, 642
MboII GAAGA 2 cut(s) 44, 460
MflI RGATCY 2 cut(s) 44, 192
MhlI GDGCHC 2 cut(s) 168, 310
MmeI TCCRAC 4 cut(s) 381, 492, 520, 549
MnlI CCTC 2 cut(s) 87, 351
MseI TTAA 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 4 cut(s) 74, 113, 370, 406
MspR9I CCNGG 2 cut(s) 214, 407
MvaI CCWGG 1 cut(s) 214
MwoI GCNNNNNNNGC 2 cut(s) 435, 587
NciI CCSGG 1 cut(s) 407
NdeII GATC 3 cut(s) 44, 192, 642
NlaIII CATG 3 cut(s) 82, 393, 429
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 2 cut(s) 105, 664
PciSI GCTCTTC 1 cut(s) 27
PflMI CCANNNNNTGG 2 cut(s) 219, 400
PinAI ACCGGT 1 cut(s) 112
PkrI GCNGC 7 cut(s) 14, 17, 273, 276, 318, 431, 673
Psp124BI GAGCTC 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 4 cut(s) 367, 372, 403, 408
PsuI RGATCY 2 cut(s) 44, 192
SacI GAGCTC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 677
SatI GCNGC 7 cut(s) 13, 16, 272, 275, 317, 430, 672
Sau3AI GATC 3 cut(s) 44, 192, 642
Sau96I GGNCC 4 cut(s) 367, 372, 403, 408
ScrFI CCNGG 2 cut(s) 214, 407
SduI GDGCHC 2 cut(s) 168, 310
SetI ASST 9 cut(s) 209, 310, 321, 329, 362, 377, 524, 575, 603
SfaNI GCATC 2 cut(s) 27, 253
SinI GGWCC 2 cut(s) 372, 403
SsiI CCGC 7 cut(s) 12, 91, 130, 245, 271, 274, 669
SspMI CTAG 3 cut(s) 324, 341, 593
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 212, 405
TaaI ACNGT 3 cut(s) 227, 302, 530
TaiI ACGT 1 cut(s) 209
TauI GCSGC 3 cut(s) 15, 274, 277
Tru1I TTAA 1 cut(s) 677
Tru9I TTAA 1 cut(s) 677
TscAI CASTG 5 cut(s) 92, 289, 401, 496, 553
TseFI GTSAC 2 cut(s) 105, 664
TseI GCWGC 4 cut(s) 15, 316, 429, 671
Tsp45I GTSAC 2 cut(s) 105, 664
TspGWI ACGGA 2 cut(s) 110, 647
TspRI CASTG 5 cut(s) 92, 289, 401, 496, 553
Van91I CCANNNNNTGG 2 cut(s) 219, 400
VpaK11BI GGWCC 2 cut(s) 372, 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XspI CTAG 3 cut(s) 324, 341, 593
ZraI GACGTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.