Rorug04G0254300

DNA-directed RNA polymerase III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0018712
Physical Location & Seq
Forward (+)
42737349 .. 42742111
4763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0254300.1

Sequence Viewer

Length: 1707 bp
ATGGAGATGGAATCGGTTGAAGAAAACCGCCAAGTAGATTTCAATGAGGATGAATATGGTGAGCCCTCATTTTCTCTAGAAGCTTCTGCTATGTGTGACGTCTTTAGTGATCCTCAGGTACTTCCTAGAGTTGGAAATGAATATCAGGTTGAAATTCCCCCTCTATCAGCTGCATCTTACTCTTCTGGGCTTATAAACCATCCAACTAATGCAAAAATCACTGCTGATGATCCCTTTGAGTTTCTAATGGGGTTACCCATTCCAGTAATGTGGATATATGAGGAAGTTAAGAGTAAGGAGCATGATTCAGAGGTAGCTTTTGATGTTGCAGTTGGTGTAGCCAACAAAAATGGTTCTTTAAAATCTAAACGCATCAAACAGATAGAAAGTGACAACTCAAAACTCAGTGCTGAGCCAATGGATATTAAGTCTGCTCATGGGATGATATCAAGTAAGTCGGTGAAGTTGGGTTTGAAACAAGAAATGATGAGTGAAATGCATGAAAAGCGTAAGAGAAGACAGTACAGTCTGGTTCCTGGTTCCTCAGTTGACCACTGGAGTGACATAGAAAAAGATAGTTTTCTTCTTGGTTTATATATATTTGGGAAGAACCTCCTTCAGGTGAAGAAATTTATTGGGAGTAAACAGATGGGCGATATATTGTCATTCTACTATGGAAAATTTTATAGGTCTTGCAGCTACATAAGATGGGCAGAACATAAAAGAAAGAAGAGCAAAAGAGGTAAACTAGGAGAAAATTTATTTACAGGATTAAGGCAAGAAGTGTTGTTATCTCGTTTGCTTCCCCACTTGTCAGAAGAAGACCAAACTACTTTACTTGAGGTATCAAAGACATATCGAGAAGAAAAAATGTTACAAGAAGCATATGTATTCATTGTAAAGGCTAAAGTCGGACTGAGTGCTTTTGTACAGGCAGTAGGAATCGGTAAAGGGAAGCAAGATCTAACAGTGCTTCGGAAAAGACCGGCTCCTTTAGCTCACGGTACCCCTGTCCCGAACTGTGGTTTAGTTTGTCCAGAGTTGAAGGCTGCCATTACTCCCAGTAAAAGATCAAAGAAGGGCAAGAAGCGAGGAGCTGATGAAGTCCCCCAAGCTGAACTGAGAAGAGATAAAAGGGTGAGCTCTGGGCCTTCTACAACCAGCTCAGATCAAGCTTCTCTGGTTCCCTTCGTCAATTCTATAATGAAGTCTGTTAGAGAGCCAGCCAAGGGTATGAACCCTATCGAGCATCTCCAGAGGTCCTATGCCCAGGCCACGCAGGTCTTGTTCGACCAATTTCATGCCTATGGAGATGTCATTGGGGAGTTACAGAAGGAGAGCATTACAAGAGCCGAGCTGGAGAAGGAACGGGCCAAAGTGAAAGATCTGGAGACACAGCTTGGTTTTCTCAAAAGAGACTTGGAGAAGACAAAGACTAAGGTGGATGAGCTGAATGCTGAGCTCATCTGTGGCTCAGGGGGTGAAGAGGAGAGGTATAAGGCTGCCGTAGAGCACTTCAAGAAGTCCCAGGAGTTTACCCAGATGAAAAATGACCTGATTAACCATGGGATCATTCGGTGTATGAATATCATGAATGCCTACTTCCCCAACCTGGAATGGAAATGCTTGGATGGTCCACCTCCTGATGATAAAGATTTGGAGGCGAAAGAGGATGATCGAACACGAAGGAAGCAATCAGCAGGGTGA

Protein Analysis

568

Amino Acids

64.08

Weight (kDa)

8.38

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7952 PF25826 186 - 316 3.8e-54 Domain of unknown function (DUF7952)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24970
malus_domestica MD13G1107600.v1.1
prunus_persica Prupe.1G238800_v2.0.a1
pyrus_communis pycom16g09170
rosa_multiflora Rmu_sc0004965.1_g000022
rosa_roxburghii Rroxscaffold_5G00373580
rosa_rugosa Rorug04G0254300
rosa_samantha Rh4CG334300
rosa_wichuraiana Rw4G027090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
AasI GACNNNNNNGTC 1 cut(s) 525
AatII GACGTC 1 cut(s) 102
Acc36I ACCTGC 1 cut(s) 1270
Acc65I GGTACC 1 cut(s) 1004
AccB1I GGYRCC 1 cut(s) 1004
AciI CCGC 1 cut(s) 28
AclWI GGATC 3 cut(s) 104, 224, 1577
AcsI RAATTY 4 cut(s) 153, 629, 680, 757
AcuI CTGAAG 1 cut(s) 602
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 4 cut(s) 120, 524, 930, 1006
AfiI CCNNNNNNNGG 5 cut(s) 131, 619, 1022, 1229, 1612
AgsI TTSAA 6 cut(s) 20, 43, 152, 475, 1045, 1519
AjnI CCWGG 4 cut(s) 535, 1269, 1527, 1611
AleI CACNNNNGTG 1 cut(s) 558
Alw21I GWGCWC 3 cut(s) 1145, 1464, 1515
Alw26I GTCTC 2 cut(s) 1385, 1410
AlwI GGATC 3 cut(s) 104, 224, 1577
AoxI GGCC 3 cut(s) 1148, 1272, 1371
ApeKI GCWGC 4 cut(s) 170, 696, 1049, 1502
ApoI RAATTY 4 cut(s) 153, 629, 680, 757
Asp718I GGTACC 1 cut(s) 1004
AspS9I GGNCC 4 cut(s) 1148, 1260, 1371, 1634
AsuHPI GGTGA 5 cut(s) 71, 472, 634, 1150, 1493
AvaII GGWCC 2 cut(s) 1260, 1634
AxyI CCTNAGG 1 cut(s) 114
BaeI ACNNNNGTAYC 2 cut(s) 996, 1029
BanI GGYRCC 1 cut(s) 1004
BanII GRGCYC 3 cut(s) 66, 1145, 1464
BbsI GAAGAC 3 cut(s) 523, 828, 1433
Bbv12I GWGCWC 3 cut(s) 1145, 1464, 1515
BbvI GCAGC 4 cut(s) 157, 708, 1036, 1489
BccI CCATC 4 cut(s) 207, 643, 702, 1625
BceAI ACGGC 1 cut(s) 1490
BciT130I CCWGG 4 cut(s) 537, 1271, 1529, 1613
BcoDI GTCTC 2 cut(s) 1385, 1410
BfaI CTAG 3 cut(s) 77, 126, 749
BfuAI ACCTGC 1 cut(s) 1270
BglII AGATCT 2 cut(s) 961, 1384
BisI GCNGC 4 cut(s) 171, 697, 1050, 1503
BlpI GCTNAGC 2 cut(s) 411, 1458
BlsI GCNGC 4 cut(s) 172, 698, 1051, 1504
Bme1390I CCNGG 4 cut(s) 537, 1271, 1529, 1613
Bme18I GGWCC 2 cut(s) 1260, 1634
BmgT120I GGNCC 4 cut(s) 1148, 1260, 1371, 1634
BmiI GGNNCC 5 cut(s) 534, 541, 990, 1006, 1185
BmrFI CCNGG 4 cut(s) 537, 1271, 1529, 1613
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 3 cut(s) 182, 381, 1258
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 3 cut(s) 523, 828, 1433
BpmI CTGGAG 4 cut(s) 577, 1238, 1379, 1409
Bpu10I CCTNAGC 1 cut(s) 1474
Bpu1102I GCTNAGC 2 cut(s) 411, 1458
BpuEI CTTGAG 1 cut(s) 860
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 4 cut(s) 1227, 1269, 1527, 1564
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 619, 1022, 1229, 1612
Bse118I RCCGGY 1 cut(s) 985
Bse1I ACTGG 3 cut(s) 263, 560, 1062
Bse21I CCTNAGG 1 cut(s) 114
BseBI CCWGG 4 cut(s) 537, 1271, 1529, 1613
BseDI CCNNGG 4 cut(s) 1227, 1269, 1527, 1564
BseGI GGATG 6 cut(s) 55, 199, 447, 1450, 1636, 1678
BseLI CCNNNNNNNGG 5 cut(s) 131, 619, 1022, 1229, 1612
BseMII CTCAG 9 cut(s) 128, 402, 418, 558, 908, 1112, 1179, 1449, 1488
BseNI ACTGG 3 cut(s) 263, 560, 1062
BseRI GAGGAG 2 cut(s) 1107, 1502
BseXI GCAGC 4 cut(s) 157, 708, 1036, 1489
BshFI GGCC 3 cut(s) 1150, 1274, 1373
BshNI GGYRCC 1 cut(s) 1004
BsiHKAI GWGCWC 3 cut(s) 1145, 1464, 1515
BsiSI CCGG 1 cut(s) 986
BslFI GGGAC 3 cut(s) 998, 1091, 1510
BslI CCNNNNNNNGG 5 cut(s) 131, 619, 1022, 1229, 1612
BsmAI GTCTC 2 cut(s) 1385, 1410
BsmFI GGGAC 3 cut(s) 998, 1091, 1510
BsmI GAATGC 2 cut(s) 1459, 1600
BsnI GGCC 3 cut(s) 1150, 1274, 1373
Bsp1286I GDGCHC 4 cut(s) 66, 1145, 1464, 1515
Bsp1407I TGTACA 1 cut(s) 928
Bsp143I GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
Bsp1720I GCTNAGC 2 cut(s) 411, 1458
Bsp19I CCATGG 1 cut(s) 1564
BspACI CCGC 1 cut(s) 28
BspANI GGCC 3 cut(s) 1150, 1274, 1373
BspCNI CTCAG 9 cut(s) 127, 403, 417, 557, 909, 1113, 1178, 1450, 1487
BspHI TCATGA 1 cut(s) 1590
BspLI GGNNCC 5 cut(s) 534, 541, 990, 1006, 1185
BspMI ACCTGC 1 cut(s) 1270
BspPI GGATC 3 cut(s) 104, 224, 1577
BspQI GCTCTTC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 1004
BsrFI RCCGGY 1 cut(s) 985
BsrGI TGTACA 1 cut(s) 928
BsrI ACTGG 3 cut(s) 263, 560, 1062
BssAI RCCGGY 1 cut(s) 985
BssECI CCNNGG 4 cut(s) 1227, 1269, 1527, 1564
BssMI GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 2 cut(s) 1227, 1564
Bst2UI CCWGG 4 cut(s) 537, 1271, 1529, 1613
Bst4CI ACNGT 5 cut(s) 522, 527, 970, 1004, 1022
Bst6I CTCTTC 4 cut(s) 187, 725, 1120, 1479
BstACI GRCGYC 1 cut(s) 99
BstAUI TGTACA 1 cut(s) 928
BstC8I GCNNGC 1 cut(s) 1224
BstDSI CCRYGG 1 cut(s) 1564
BstEII GGTNACC 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 617
BstF5I GGATG 6 cut(s) 55, 199, 447, 1450, 1636, 1678
BstKTI GATC 8 cut(s) 112, 232, 964, 1073, 1171, 1387, 1572, 1678
BstMAI GTCTC 2 cut(s) 1385, 1410
BstMBI GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
BstMWI GCNNNNNNNGC 2 cut(s) 505, 995
BstNI CCWGG 4 cut(s) 537, 1271, 1529, 1613
BstPI GGTNACC 1 cut(s) 252
BstSCI CCNGG 4 cut(s) 535, 1269, 1527, 1611
BstV1I GCAGC 4 cut(s) 157, 708, 1036, 1489
BstV2I GAAGAC 3 cut(s) 523, 828, 1433
BstX2I RGATCY 2 cut(s) 961, 1384
BstXI CCANNNNNNTGG 1 cut(s) 270
BstYI RGATCY 2 cut(s) 961, 1384
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 3 cut(s) 1150, 1274, 1373
BtgI CCRYGG 1 cut(s) 1564
BtsCI GGATG 6 cut(s) 55, 199, 447, 1450, 1636, 1678
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 4 cut(s) 219, 412, 553, 975
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 1 cut(s) 1224
CciI TCATGA 1 cut(s) 1590
Cfr10I RCCGGY 1 cut(s) 985
Cfr13I GGNCC 4 cut(s) 1148, 1260, 1371, 1634
Csp6I GTAC 4 cut(s) 119, 523, 929, 1005
CviAII CATG 6 cut(s) 302, 437, 500, 1301, 1565, 1591
CviQI GTAC 4 cut(s) 119, 523, 929, 1005
DpnI GATC 8 cut(s) 111, 231, 963, 1072, 1170, 1386, 1571, 1677
DpnII GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
DraI TTTAAA 1 cut(s) 360
DrdI GACNNNNNNGTC 1 cut(s) 525
DseDI GACNNNNNNGTC 1 cut(s) 525
Eam1104I CTCTTC 4 cut(s) 187, 725, 1120, 1479
EarI CTCTTC 4 cut(s) 187, 725, 1120, 1479
Ecl136II GAGCTC 2 cut(s) 1143, 1462
Eco130I CCWWGG 2 cut(s) 1227, 1564
Eco24I GRGCYC 3 cut(s) 66, 1145, 1464
Eco32I GATATC 1 cut(s) 447
Eco47I GGWCC 2 cut(s) 1260, 1634
Eco53kI GAGCTC 2 cut(s) 1143, 1462
Eco57I CTGAAG 1 cut(s) 602
Eco81I CCTNAGG 1 cut(s) 114
Eco91I GGTNACC 1 cut(s) 252
EcoICRI GAGCTC 2 cut(s) 1143, 1462
EcoNI CCTNNNNNAGG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 1260
EcoO65I GGTNACC 1 cut(s) 252
EcoRII CCWGG 4 cut(s) 535, 1269, 1527, 1611
EcoRV GATATC 1 cut(s) 447
EcoT14I CCWWGG 2 cut(s) 1227, 1564
EcoT22I ATGCAT 1 cut(s) 501
EcoT38I GRGCYC 3 cut(s) 66, 1145, 1464
ErhI CCWWGG 2 cut(s) 1227, 1564
FaeI CATG 6 cut(s) 305, 440, 503, 1304, 1568, 1594
FaqI GGGAC 3 cut(s) 998, 1091, 1510
FatI CATG 6 cut(s) 301, 436, 499, 1300, 1564, 1590
FauNDI CATATG 1 cut(s) 886
Fnu4HI GCNGC 4 cut(s) 171, 697, 1050, 1503
FokI GGATG 6 cut(s) 62, 186, 454, 1457, 1643, 1685
FriOI GRGCYC 3 cut(s) 66, 1145, 1464
Fsp4HI GCNGC 4 cut(s) 171, 697, 1050, 1503
FspBI CTAG 3 cut(s) 77, 126, 749
GluI GCNGC 4 cut(s) 171, 697, 1050, 1503
GsuI CTGGAG 4 cut(s) 577, 1238, 1379, 1409
HaeIII GGCC 3 cut(s) 1150, 1274, 1373
HapII CCGG 1 cut(s) 986
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 6 cut(s) 305, 440, 503, 1304, 1568, 1594
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 2 cut(s) 81, 1173
HinfI GANTC 3 cut(s) 11, 305, 942
HpaII CCGG 1 cut(s) 986
HphI GGTGA 5 cut(s) 71, 472, 634, 1150, 1493
Hpy166II GTNNAC 5 cut(s) 550, 644, 746, 1536, 1637
Hpy188I TCNGA 5 cut(s) 310, 817, 914, 978, 1168
Hpy188III TCNNGA 9 cut(s) 77, 860, 1015, 1037, 1255, 1388, 1519, 1591, 1643
Hpy8I GTNNAC 5 cut(s) 550, 644, 746, 1536, 1637
HpyAV CCTTC 8 cut(s) 626, 1039, 1072, 1161, 1198, 1327, 1357, 1680
HpyCH4III ACNGT 5 cut(s) 522, 527, 970, 1004, 1022
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 5 cut(s) 173, 212, 329, 499, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 995
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 6 cut(s) 305, 440, 503, 1304, 1568, 1594
KpnI GGTACC 1 cut(s) 1008
Kzo9I GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
LguI GCTCTTC 1 cut(s) 725
LmnI GCTCC 3 cut(s) 298, 994, 1094
Lsp1109I GCAGC 4 cut(s) 157, 708, 1036, 1489
LweI GCATC 3 cut(s) 182, 381, 1258
MaeI CTAG 3 cut(s) 77, 126, 749
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 6 cut(s) 95, 252, 389, 560, 873, 1326
MalI GATC 8 cut(s) 111, 231, 963, 1072, 1170, 1386, 1571, 1677
MboI GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
MflI RGATCY 2 cut(s) 961, 1384
MhlI GDGCHC 4 cut(s) 66, 1145, 1464, 1515
MluCI AATT 6 cut(s) 153, 629, 680, 757, 1195, 1295
MmeI TCCRAC 3 cut(s) 112, 227, 892
Mph1103I ATGCAT 1 cut(s) 501
MseI TTAA 5 cut(s) 288, 359, 426, 773, 1560
MslI CAYNNNNRTG 2 cut(s) 558, 1305
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 4 cut(s) 537, 1271, 1529, 1613
Mva1269I GAATGC 2 cut(s) 1459, 1600
MvaI CCWGG 4 cut(s) 537, 1271, 1529, 1613
MwoI GCNNNNNNNGC 2 cut(s) 505, 995
NcoI CCATGG 1 cut(s) 1564
NdeI CATATG 1 cut(s) 886
NdeII GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
NlaIII CATG 6 cut(s) 305, 440, 503, 1304, 1568, 1594
NlaIV GGNNCC 5 cut(s) 534, 541, 990, 1006, 1185
NmeAIII GCCGAG 1 cut(s) 1378
NmuCI GTSAC 3 cut(s) 95, 389, 560
NsiI ATGCAT 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 558
PagI TCATGA 1 cut(s) 1590
PciSI GCTCTTC 1 cut(s) 725
PctI GAATGC 2 cut(s) 1459, 1600
PfeI GAWTC 3 cut(s) 11, 305, 942
PkrI GCNGC 4 cut(s) 172, 698, 1051, 1504
PpuMI RGGWCCY 1 cut(s) 1260
PsiI TTATAA 1 cut(s) 194
Psp124BI GAGCTC 2 cut(s) 1145, 1464
Psp5II RGGWCCY 1 cut(s) 1260
Psp6I CCWGG 4 cut(s) 535, 1269, 1527, 1611
PspEI GGTNACC 1 cut(s) 252
PspGI CCWGG 4 cut(s) 535, 1269, 1527, 1611
PspN4I GGNNCC 5 cut(s) 534, 541, 990, 1006, 1185
PspPI GGNCC 4 cut(s) 1148, 1260, 1371, 1634
PspPPI RGGWCCY 1 cut(s) 1260
PsuI RGATCY 2 cut(s) 961, 1384
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 4 cut(s) 120, 524, 930, 1006
RsaNI GTAC 4 cut(s) 119, 523, 929, 1005
RseI CAYNNNNRTG 2 cut(s) 558, 1305
SacI GAGCTC 2 cut(s) 1145, 1464
SapI GCTCTTC 1 cut(s) 725
SaqAI TTAA 5 cut(s) 288, 359, 426, 773, 1560
SatI GCNGC 4 cut(s) 171, 697, 1050, 1503
Sau3AI GATC 8 cut(s) 109, 229, 961, 1070, 1168, 1384, 1569, 1675
Sau96I GGNCC 4 cut(s) 1148, 1260, 1371, 1634
ScrFI CCNGG 4 cut(s) 537, 1271, 1529, 1613
SduI GDGCHC 4 cut(s) 66, 1145, 1464, 1515
SfaNI GCATC 3 cut(s) 182, 381, 1258
SinI GGWCC 2 cut(s) 1260, 1634
SmiMI CAYNNNNRTG 2 cut(s) 558, 1305
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 6 cut(s) 153, 629, 680, 757, 1195, 1295
SsiI CCGC 1 cut(s) 28
SspMI CTAG 3 cut(s) 77, 126, 749
SstI GAGCTC 2 cut(s) 1145, 1464
StyD4I CCNGG 4 cut(s) 535, 1269, 1527, 1611
StyI CCWWGG 2 cut(s) 1227, 1564
TaaI ACNGT 5 cut(s) 522, 527, 970, 1004, 1022
TaiI ACGT 1 cut(s) 102
TaqI TCGA 4 cut(s) 859, 1245, 1290, 1678
TasI AATT 6 cut(s) 153, 629, 680, 757, 1195, 1295
TatI WGTACW 2 cut(s) 522, 928
TfiI GAWTC 3 cut(s) 11, 305, 942
Tru1I TTAA 5 cut(s) 288, 359, 426, 773, 1560
Tru9I TTAA 5 cut(s) 288, 359, 426, 773, 1560
TscAI CASTG 4 cut(s) 226, 412, 560, 975
TseFI GTSAC 3 cut(s) 95, 389, 560
TseI GCWGC 4 cut(s) 170, 696, 1049, 1502
Tsp45I GTSAC 3 cut(s) 95, 389, 560
TspRI CASTG 4 cut(s) 226, 412, 560, 975
VpaK11BI GGWCC 2 cut(s) 1260, 1634
XagI CCTNNNNNAGG 1 cut(s) 617
XapI RAATTY 4 cut(s) 153, 629, 680, 757
XbaI TCTAGA 1 cut(s) 76
XspI CTAG 3 cut(s) 77, 126, 749
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.