Rorug05G0018800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017528
Physical Location & Seq
Forward (+)
1254376 .. 1255014
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0018800.1

Sequence Viewer

Length: 477 bp
ATGAAAGGCTCCATCCAAGTCTTCTTTTTGCTAGCCATGCTAATGGCTTTAGCCGCCATTACTCTTTCCGCATCAACCCCGGAGGAAGATCGAGATGAATGGTTCTCCGATGAGGAAACAGATACTCCAAATGAACCATCTGATGATCTCCCTACGACCTTAAGCCAACCAAAAACTTCTCTCAGGGGAGCAAGCCGCTTCCTTGCCACTCGGGCTGTTGCGACTACATGTGACAAAAACCCTAAGGTGTGTAAGGCTGCAGGCAGCACGGGGCGAGATTGCTGCAAGAAGAAGTGTGTGGATCTGAAGACGGACAGAGTCAATTGCGGCAAGTGTGGGAAGAAATGCAAGTACTCGGAGATATGCTGCAAAGGAAAGTGTCTGAATCCAATGTCTGACAAGAAAAACTGTGGGAGCTGCAACAACAAGTGCATGAAAGGCAGTTCGTGTGTGTACGGAATATGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.1

Weight (kDa)

8.66

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 75 - 158 6.7e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18395
fragaria_vesca FvH4_3g05210
prunus_persica Prupe.4G083800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0014511
rosa_laevigata RLG00000032119
rosa_multiflora Rmu_sc0013570.1_g000004
rosa_roxburghii Rroxscaffold_1G00062020
rosa_rugosa Rorug05G0018800
rosa_samantha Rh5BG111000 Rh5CG122600 Rh5DG109900
rosa_wichuraiana Rw5G009890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 54, 69, 196, 327
AclWI GGATC 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 2 cut(s) 353, 455
AflII CTTAAG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 227
AluBI AGCT 2 cut(s) 417, 468
AluI AGCT 2 cut(s) 417, 468
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 210
ApeKI GCWGC 6 cut(s) 257, 264, 282, 366, 417, 465
AsuC2I CCSGG 1 cut(s) 80
AsuNHI GCTAGC 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 210
AxyI CCTNAGG 1 cut(s) 243
BbsI GAAGAC 2 cut(s) 13, 314
BbvI GCAGC 5 cut(s) 244, 269, 276, 353, 404
BccI CCATC 2 cut(s) 20, 145
BcgI CGANNNNNNTGC 2 cut(s) 264, 298
BcnI CCSGG 1 cut(s) 80
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 258
BfrI CTTAAG 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
BlsI GCNGC 9 cut(s) 55, 197, 259, 266, 284, 329, 368, 419, 467
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 80
BmtI GCTAGC 1 cut(s) 35
BpiI GAAGAC 2 cut(s) 13, 314
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bse21I CCTNAGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 196
BseXI GCAGC 5 cut(s) 244, 269, 276, 353, 404
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 210
Bsp143I GATC 3 cut(s) 88, 145, 301
BspACI CCGC 4 cut(s) 54, 69, 196, 327
BspCNI CTCAG 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 10
BspMAI CTGCAG 1 cut(s) 262
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 309
BspTI CTTAAG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 88, 145, 301
Bst4CI ACNGT 1 cut(s) 410
BstAFI CTTAAG 1 cut(s) 160
BstC8I GCNNGC 3 cut(s) 33, 193, 262
BstDEI CTNAG 2 cut(s) 182, 243
BstF5I GGATG 1 cut(s) 12
BstKTI GATC 3 cut(s) 91, 148, 304
BstMBI GATC 3 cut(s) 88, 145, 301
BstMWI GCNNNNNNNGC 4 cut(s) 37, 53, 212, 438
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 1 cut(s) 258
BstV1I GCAGC 5 cut(s) 244, 269, 276, 353, 404
BstV2I GAAGAC 2 cut(s) 13, 314
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 12
Cac8I GCNNGC 3 cut(s) 33, 193, 262
Csp6I GTAC 2 cut(s) 352, 454
CviAII CATG 3 cut(s) 37, 228, 433
CviQI GTAC 2 cut(s) 352, 454
DdeI CTNAG 2 cut(s) 182, 243
DpnI GATC 3 cut(s) 90, 147, 303
DpnII GATC 3 cut(s) 88, 145, 301
Eco57I CTGAAG 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 475
FaeI CATG 3 cut(s) 40, 231, 436
FaiI YATR 7 cut(s) 38, 229, 364, 434, 463, 471, 475
FatI CATG 3 cut(s) 36, 227, 432
Fnu4HI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
Fsp4HI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
FspBI CTAG 1 cut(s) 32
GluI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
HapII CCGG 1 cut(s) 80
Hin1II CATG 3 cut(s) 40, 231, 436
HinfI GANTC 2 cut(s) 318, 385
HpaII CCGG 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 6 cut(s) 109, 142, 306, 358, 384, 397
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 1 cut(s) 454
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 8 cut(s) 260, 285, 348, 369, 420, 432, 465, 473
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 53, 212, 438
HpyF3I CTNAG 2 cut(s) 182, 243
Hsp92II CATG 3 cut(s) 40, 231, 436
Kzo9I GATC 3 cut(s) 88, 145, 301
LmnI GCTCC 3 cut(s) 14, 188, 414
LpnPI CCDG 3 cut(s) 93, 169, 246
Lsp1109I GCAGC 5 cut(s) 244, 269, 276, 353, 404
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 230
MalI GATC 3 cut(s) 90, 147, 303
MboI GATC 3 cut(s) 88, 145, 301
MboII GAAGA 5 cut(s) 13, 98, 301, 319, 352
MfeI CAATTG 1 cut(s) 322
MflI RGATCY 1 cut(s) 301
MluCI AATT 1 cut(s) 322
MlyI GAGTC 1 cut(s) 327
MnlI CCTC 2 cut(s) 76, 106
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 160
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 80
MunI CAATTG 1 cut(s) 322
MwoI GCNNNNNNNGC 4 cut(s) 37, 53, 212, 438
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 88, 145, 301
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 3 cut(s) 40, 231, 436
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 230
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 231
PciI ACATGT 1 cut(s) 227
PfeI GAWTC 1 cut(s) 385
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 9 cut(s) 55, 197, 259, 266, 284, 329, 368, 419, 467
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PscI ACATGT 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 2 cut(s) 353, 455
RsaNI GTAC 2 cut(s) 352, 454
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
Sau3AI GATC 3 cut(s) 88, 145, 301
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 80
SetI ASST 4 cut(s) 161, 249, 419, 470
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 1 cut(s) 322
SsiI CCGC 4 cut(s) 54, 69, 196, 327
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 91
TasI AATT 1 cut(s) 322
TatI WGTACW 1 cut(s) 351
TauI GCSGC 3 cut(s) 56, 198, 330
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 6 cut(s) 257, 264, 282, 366, 417, 465
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 4 cut(s) 17, 111, 147, 449
TspGWI ACGGA 2 cut(s) 326, 471
Tth111I GACNNNGTC 1 cut(s) 317
Vha464I CTTAAG 1 cut(s) 160
XceI RCATGY 1 cut(s) 231
XspI CTAG 1 cut(s) 32
ZrmI AGTACT 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.