Rorug05G0481600

polyamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017639
Physical Location & Seq
Reverse (-)
66265789 .. 66268118
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0481600.1

Sequence Viewer

Length: 525 bp
ATGGAGAAGGAGCTGGTGGAGGTGTTTGAGGAGGTGATGAAGGCGGCGGAACCCACGAAATCGTGTGACGGAGACGCCGAGGAAAGCAATTCCTTGAGCCTTGGCCCGGTTGGGGATTACATTGTTTATTTTCATTTCATGGATACTCTCGGCTTGAAGAGTAATTGCTTCTTTGAATCCTCTTGTAGCTGGAGGATAGATATGGGTCTCGAACTAACCCGTCATGTACAAGCCAAAGGTTATCGAATTTTGAGATATTTGTTGAGGGTCTTCTACATGTTCTTCGAGTCTTGCACCAATAAGAAGGACCCTGTTGTGACATGGCTGACTGGAGGCCCAGGTTGCAGTAGTGAATTGGCTGTGTTCTATGAAAATGGTCCTTTCACAATTGTGAATAACTTGTCTCTTGTATGGAATGAGTATGGTTGGGATAAGGCATCTAATCTGTTGTATGTTGACCAGCCTATTGGTACGGGATTCAGTTATAGTTCTGACAGACGTGATATTTGCCACTCTGAAATTTGA

Protein Analysis

174

Amino Acids

19.84

Weight (kDa)

4.71

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 89 - 169 3e-33 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45281
malus_domestica MD11G1009900.v1.1
prunus_persica Prupe.6G006400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0082951
rosa_laevigata RLG00000037004
rosa_multiflora Rmu_sc0017035.1_g000002
rosa_roxburghii Rroxscaffold_1G00001470
rosa_rugosa Rorug05G0481600
rosa_samantha Rh5BG558200 Rh5CG581100 Rh5DG567000
rosa_wichuraiana Rw5G049740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 44, 47
AcsI RAATTY 2 cut(s) 246, 519
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 228, 472
AfiI CCNNNNNNNGG 2 cut(s) 106, 112
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 157, 176
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 1 cut(s) 337
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 2 cut(s) 13, 189
AluI AGCT 2 cut(s) 13, 189
Alw26I GTCTC 3 cut(s) 66, 212, 408
AoxI GGCC 2 cut(s) 103, 334
ApoI RAATTY 2 cut(s) 246, 519
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
AspS9I GGNCC 4 cut(s) 104, 307, 335, 377
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 2 cut(s) 307, 377
BbsI GAAGAC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 136
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 3 cut(s) 66, 212, 408
BfuI GTATCC 1 cut(s) 136
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 107, 339
Bme18I GGWCC 2 cut(s) 307, 377
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 4 cut(s) 104, 307, 335, 377
BmiI GGNNCC 2 cut(s) 51, 309
BmrFI CCNGG 2 cut(s) 107, 339
BmsI GCATC 1 cut(s) 446
BpiI GAAGAC 1 cut(s) 262
BpmI CTGGAG 2 cut(s) 211, 351
BpuEI CTTGAG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 78, 100, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 112
Bse1I ACTGG 1 cut(s) 334
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 3 cut(s) 78, 100, 337
BseLI CCNNNNNNNGG 2 cut(s) 106, 112
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 44
BshFI GGCC 2 cut(s) 105, 336
BsiSI CCGG 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 106, 112
BsmAI GTCTC 3 cut(s) 66, 212, 408
BsmBI CGTCTC 1 cut(s) 66
BsnI GGCC 2 cut(s) 105, 336
Bso31I GGTCTC 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 226
BspACI CCGC 2 cut(s) 44, 47
BspANI GGCC 2 cut(s) 105, 336
BspLI GGNNCC 2 cut(s) 51, 309
BspTNI GGTCTC 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 3 cut(s) 78, 100, 337
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 339
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 226
BstMAI GTCTC 3 cut(s) 66, 212, 408
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 2 cut(s) 105, 337
BstV2I GAAGAC 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 467
BsuI GTATCC 1 cut(s) 136
BsuRI GGCC 2 cut(s) 105, 336
BtrI CACGTC 1 cut(s) 500
Cfr13I GGNCC 4 cut(s) 104, 307, 335, 377
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 227, 471
CviAII CATG 4 cut(s) 139, 224, 277, 321
CviQI GTAC 2 cut(s) 227, 471
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 100
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 307, 377
EcoO109I RGGNCCY 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 4 cut(s) 142, 227, 280, 324
FatI CATG 4 cut(s) 138, 223, 276, 320
Fnu4HI GCNGC 1 cut(s) 45
Fsp4HI GCNGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 45
GsuI CTGGAG 2 cut(s) 211, 351
HaeIII GGCC 2 cut(s) 105, 336
HapII CCGG 1 cut(s) 107
HgaI GACGC 1 cut(s) 83
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 4 cut(s) 142, 227, 280, 324
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 3 cut(s) 176, 287, 477
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 2 cut(s) 493, 517
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 2 cut(s) 34, 298
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 2 cut(s) 294, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 499
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 4 cut(s) 142, 227, 280, 324
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 7 cut(s) 120, 175, 315, 324, 324, 351, 473
LweI GCATC 1 cut(s) 446
MaeII ACGT 1 cut(s) 499
MaeIII GTNAC 2 cut(s) 65, 316
MboII GAAGA 3 cut(s) 169, 262, 274
MfeI CAATTG 1 cut(s) 387
MluCI AATT 6 cut(s) 88, 163, 246, 353, 387, 519
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 8 cut(s) 13, 22, 25, 73, 186, 190, 258, 326
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 107, 339
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 342
NciI CCSGG 1 cut(s) 107
NlaIII CATG 4 cut(s) 142, 227, 280, 324
NlaIV GGNNCC 2 cut(s) 51, 309
NmeAIII GCCGAG 2 cut(s) 103, 129
NmuCI GTSAC 2 cut(s) 65, 316
NspI RCATGY 1 cut(s) 280
OliI CACNNNNGTG 1 cut(s) 389
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 2 cut(s) 176, 477
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 307
PscI ACATGT 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 51, 309
PspPI GGNCC 4 cut(s) 104, 307, 335, 377
PspPPI RGGWCCY 1 cut(s) 307
RsaI GTAC 2 cut(s) 228, 472
RsaNI GTAC 2 cut(s) 227, 471
RseI CAYNNNNRTG 1 cut(s) 389
SatI GCNGC 1 cut(s) 45
Sau96I GGNCC 4 cut(s) 104, 307, 335, 377
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 107, 339
SetI ASST 7 cut(s) 15, 24, 36, 191, 241, 343, 502
SfaNI GCATC 1 cut(s) 446
SinI GGWCC 2 cut(s) 307, 377
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 6 cut(s) 88, 163, 246, 353, 387, 519
SsiI CCGC 2 cut(s) 44, 47
StyD4I CCNGG 2 cut(s) 105, 337
StyI CCWWGG 1 cut(s) 100
TaiI ACGT 1 cut(s) 502
TaqI TCGA 3 cut(s) 210, 244, 285
TasI AATT 6 cut(s) 88, 163, 246, 353, 387, 519
TatI WGTACW 1 cut(s) 226
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 176, 477
TseFI GTSAC 2 cut(s) 65, 316
Tsp45I GTSAC 2 cut(s) 65, 316
TspDTI ATGAA 4 cut(s) 53, 122, 127, 384
TspGWI ACGGA 1 cut(s) 84
VpaK11BI GGWCC 2 cut(s) 307, 377
XapI RAATTY 2 cut(s) 246, 519
XceI RCATGY 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.