Rorug06G0497100

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0019224
Physical Location & Seq
Forward (+)
61344102 .. 61344845
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0497100.1

Sequence Viewer

Length: 552 bp
ATGAACAAGTACCAAAGCTGTGCCTCTTGCAGACACAACCGCTCAAAATGTGTACGGGATTGTCCACTGGCCCCGTACTTCCCTTCGCACATGCACCAAAAATTCAAGAATGCCCGCAAGGTGTTCGGCGAAAGTCACCTGAAGAAGTTGCTCCGGAGCTTACCCCCTCGCAAAAGAAGCACTGCAATGAAGCACATTATAGCGGAAGCTGACATCCGAGTCGCCGACCCGGTTGGTGGAAGTTATAGTCTTATAAGGAACTTGGAGCTGAAGATTGCGGAGGAAGAGGAGGAGCTCCGCCGCTTGCGCAATGTACTTAGTGTGTTTCGTGGCGGAGGTAGTGGTAGTGTTGTGGCACAGCCTTTGGTTCAGAAACAGGGATACATTGTTCAAGAACATGATAACATCAATGATCATGTTCCAGTACTCCCTCTTGTTCCTGGTGGATTTCTTCATGCACTCCATCAAGAAAAGCGCAACAACATGTTTGATTTAAGGGATGAAGTTGTGCCGGAACATGATGTCAACCCAAATCAGGGTGAGGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

183

Amino Acids

20.83

Weight (kDa)

8.91

Isoelectric Point (pI)

71.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 6 - 102 2e-29 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019224)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g10090 FvH4_7g25270
rosa_chinensis RchiOBHm_Chr4g0392641
rosa_rugosa Rorug06G0497100
rosa_samantha Rh2AG423500 Rh3BG092300 Rh6DG346100 Rh7DG317800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AasI GACNNNNNNGTC 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 42
AccIII TCCGGA 1 cut(s) 153
AciI CCGC 7 cut(s) 40, 115, 203, 278, 298, 301, 333
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 161, 290
AfaI GTAC 5 cut(s) 11, 54, 77, 315, 426
AfiI CCNNNNNNNGG 3 cut(s) 236, 535, 536
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 2 cut(s) 106, 392
AjnI CCWGG 1 cut(s) 439
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 5 cut(s) 18, 159, 209, 268, 295
AluI AGCT 5 cut(s) 18, 159, 209, 268, 295
Alw21I GWGCWC 1 cut(s) 297
Aor13HI TCCGGA 1 cut(s) 153
AoxI GGCC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 101
AspLEI GCGC 2 cut(s) 309, 477
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 128, 551
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BccI CCATC 1 cut(s) 471
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 441
BciVI GTATCC 1 cut(s) 374
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmcAI AGTACT 1 cut(s) 426
Bme1390I CCNGG 2 cut(s) 230, 441
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 230, 441
BpuMI CCSGG 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 153
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 535, 536
Bse1I ACTGG 2 cut(s) 72, 422
Bse3DI GCAATG 2 cut(s) 192, 316
BseAI TCCGGA 1 cut(s) 153
BseBI CCWGG 1 cut(s) 441
BseGI GGATG 2 cut(s) 213, 505
BseLI CCNNNNNNNGG 3 cut(s) 236, 535, 536
BseMI GCAATG 2 cut(s) 192, 316
BseNI ACTGG 2 cut(s) 72, 422
BseRI GAGGAG 2 cut(s) 302, 305
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 3 cut(s) 154, 230, 512
BslI CCNNNNNNNGG 3 cut(s) 236, 535, 536
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 153
Bsp143I GATC 1 cut(s) 412
BspACI CCGC 7 cut(s) 40, 115, 203, 278, 298, 301, 333
BspANI GGCC 1 cut(s) 71
BspEI TCCGGA 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 192, 316
BsrI ACTGG 2 cut(s) 72, 422
BssMI GATC 1 cut(s) 412
Bst2UI CCWGG 1 cut(s) 441
Bst6I CTCTTC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 115, 305
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 2 cut(s) 213, 505
BstHHI GCGC 2 cut(s) 309, 477
BstKTI GATC 1 cut(s) 415
BstMBI GATC 1 cut(s) 412
BstMWI GCNNNNNNNGC 2 cut(s) 177, 306
BstNI CCWGG 1 cut(s) 441
BstNSI RCATGY 2 cut(s) 94, 487
BstSCI CCNGG 2 cut(s) 228, 439
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 2 cut(s) 213, 505
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 65, 180
Cac8I GCNNGC 2 cut(s) 115, 305
CfoI GCGC 2 cut(s) 309, 477
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 5 cut(s) 10, 53, 76, 314, 425
CviAII CATG 6 cut(s) 91, 398, 416, 455, 484, 518
CviJI RGCY 7 cut(s) 18, 71, 159, 209, 268, 295, 361
CviKI_1 RGCY 7 cut(s) 18, 71, 159, 209, 268, 295, 361
CviQI GTAC 5 cut(s) 10, 53, 76, 314, 425
DdeI CTNAG 1 cut(s) 317
DpnI GATC 1 cut(s) 414
DpnII GATC 1 cut(s) 412
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EciI GGCGGA 2 cut(s) 287, 348
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 161, 290
EcoICRI GAGCTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 6 cut(s) 94, 401, 419, 458, 487, 521
FaiI YATR 9 cut(s) 92, 200, 246, 254, 399, 417, 456, 485, 519
FatI CATG 6 cut(s) 90, 397, 415, 454, 483, 517
FauI CCCGC 1 cut(s) 122
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 2 cut(s) 200, 512
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 301
FspI TGCGCA 1 cut(s) 308
GlaI GCGC 2 cut(s) 308, 476
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 1 cut(s) 71
HapII CCGG 3 cut(s) 154, 230, 512
HhaI GCGC 2 cut(s) 309, 477
Hin1II CATG 6 cut(s) 94, 401, 419, 458, 487, 521
Hin6I GCGC 2 cut(s) 307, 475
HinP1I GCGC 2 cut(s) 307, 475
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 1 cut(s) 219
HpaII CCGG 3 cut(s) 154, 230, 512
HphI GGTGA 2 cut(s) 128, 551
Hpy166II GTNNAC 3 cut(s) 53, 65, 526
Hpy188I TCNGA 2 cut(s) 218, 372
Hpy188III TCNNGA 4 cut(s) 106, 154, 392, 467
Hpy8I GTNNAC 3 cut(s) 53, 65, 526
HpyAV CCTTC 1 cut(s) 93
HpyCH4V TGCA 4 cut(s) 30, 94, 185, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 306
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 6 cut(s) 94, 401, 419, 458, 487, 521
HspAI GCGC 2 cut(s) 307, 475
Kpn2I TCCGGA 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 1 cut(s) 412
LmnI GCTCC 5 cut(s) 156, 156, 265, 292, 300
MaeIII GTNAC 1 cut(s) 134
MalI GATC 1 cut(s) 414
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 412
MboII GAAGA 4 cut(s) 154, 283, 296, 443
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 1 cut(s) 101
MlyI GAGTC 1 cut(s) 228
MnlI CCTC 9 cut(s) 34, 177, 274, 280, 283, 329, 441, 535, 538
MroI TCCGGA 1 cut(s) 153
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 3 cut(s) 154, 230, 512
MspR9I CCNGG 2 cut(s) 230, 441
Mva1269I GAATGC 1 cut(s) 115
MvaI CCWGG 1 cut(s) 441
MwoI GCNNNNNNNGC 2 cut(s) 177, 306
NciI CCSGG 1 cut(s) 230
NdeII GATC 1 cut(s) 412
NlaIII CATG 6 cut(s) 94, 401, 419, 458, 487, 521
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 308
NspI RCATGY 2 cut(s) 94, 487
PciI ACATGT 1 cut(s) 483
PctI GAATGC 1 cut(s) 115
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 1 cut(s) 483
PsiI TTATAA 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 70
RsaI GTAC 5 cut(s) 11, 54, 77, 315, 426
RsaNI GTAC 5 cut(s) 10, 53, 76, 314, 425
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 1 cut(s) 412
Sau96I GGNCC 1 cut(s) 70
ScaI AGTACT 1 cut(s) 426
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 2 cut(s) 230, 441
SduI GDGCHC 1 cut(s) 297
SetI ASST 8 cut(s) 20, 123, 141, 161, 211, 270, 297, 340
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 101
SsiI CCGC 7 cut(s) 40, 115, 203, 278, 298, 301, 333
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 228, 439
TasI AATT 1 cut(s) 101
TatI WGTACW 2 cut(s) 313, 424
TauI GCSGC 1 cut(s) 303
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 2 cut(s) 72, 187
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 17, 203, 443, 516
TspRI CASTG 2 cut(s) 72, 187
XapI RAATTY 1 cut(s) 101
XceI RCATGY 2 cut(s) 94, 487
ZrmI AGTACT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.