Rorug07G0025100

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0018969
Physical Location & Seq
Reverse (-)
1796575 .. 1798782
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0025100.1

Sequence Viewer

Length: 2016 bp
ATGGTGCTCGAAAGCGTAATTCTCAAGCGCATCAAGCTCTTCGAGGCCATCCAGGCTGAGCAGCTCGCCCAACGCCAGGCCCTCCCTTCCGATCCAATCATGATTACAATATTGCCGGACGGGAAGGTGAAGGAGGGAAAGAAGTGGGTGACTTCACCTTTCGACGTTGCGAAGGAGGTTTCGAAGAGCTTGGCGTCGAATGCGTTGGTTGCTGAGGTGAATGGGGTACTGTGGGACATGAGCAGGCCTTTGGAGGGTGACTGTGAGCTGAAGCTGTTCACATTTGACAGTGATGAAGGCCGGGACACTTTCTGGCATTCTAGCGCACACATTCTCGGACAGGCACTTGAGGGTTTGTATGGATGTAAACTCTGCATTGGTCCCTGCCACACTAGTAGAGAAGAGGGTTTCTATTATGATGCATTTTATGGTGATTTGGGGTTGAAAGATGAACACTTGAAGCAAATTGAATCAGAAGCAGCCAAGGCTGTTAAGGCCAAACAGCCATTTGAGCGCATTCAAGTATCAAGGGATCAAGCACTTGAGATTTTCTCTGATAATAGATTCAAGGTTGAAATCATCAATGATCTTCCAGCTGATGACAAAAGTATCACAGTCTACAGATGTGGCTCCTTGGTTGATTTGTGTTGTGGACCGCACATACCAAACACATCATTTGTCAAAGCATTTGCATGTGTGAATGCTTCTTCTGTCTACTGGAGAGGCAATAAAGACCGTGAAAGCTTACAGAGAGTTTATGGAGTGTCTTATCCTGATAAGAAAGGTTTGCAGTTATATCTCCAGCGACGGGAAGAAGCGAAAAAATATGATCATAGATTGTTGGGTACAAAGCAGGAGCTCTTCTTTAGTCACCCACTTAGTCCAGGAAGTTGGTTCTTCCTGCCCAATGGCACCCGGATTTATAACAAGCTTATGGAGTTTTTAAAGAATCAATACAGAGAAAGAGGATACCAAGAGGTTATGTCGCCAAACATGTACAATATGCAACTTTGGGAAACATCTGGTCATGCTGCAAATTACAAAGAGAATATGTTTGTCTTTGAGATTGAGAAACAAGAGTTTGGATTGAAGCCGATGAATTGTCCCGGGCATTGTTTAATGTTTCAGCACAGAGTTCGTTCTTATAGAGAGCTTCCTCTTCGACTGGCTGACTTTGGGGTTTTGCATCGGAATGAGGCCAGTGGTGCACTCACTGGATTAACTCGTGTCAGAAGATTCCAGCAGGATGATGCTCATATCTTTTGCAGGGAGTCACAAATAAAAGATGAAGTTAAGGGCGTCTTAGACTTCATCCAGTATGTCTACAATATATTTGGCTTCACTTTTGATCTGAAGCTATCAACAAGGCCAGAAAAGTACCTTGGAGGGATAGAAACATGGGAGAAAGCTGAGGCAGCTCTTACAGATGCATTAAATGAGTTTGGCAAACCCTGGCAGATAAATAAAGGGGATGGTGCATTTTACGGACCAAAGATAGATATCAGTGTATCTGATGCATTAAACAGGAAGTTTCAGTGTGCAACATTACAGCTTGATTTTCAGCTACCTGCTCGCTTTGAGATGTATTACTCAGCAGAGGGTGAAGAAGGCAAGAGGGAGGCACCTGTTATGATACACAGAGCCATTTTGGGTTCTGTCGAGCGTATGTTTGCTATACTATTGGAGCACTACAAGGGGAAATGGCCGTTCTGGCTTAGTCCACGTCAGGCGATTGTTTGCCCTGTCTCGGACAAATCCCAGCCCTATGCTCAACAGGTCCGGGACAAGATCTTTCAGTCTGGTTATTGTGTTGATGTTGACACAAGTGATAGGACCATACAGAAAAAGGTACGAGAAGCTCAGATGGCTCAGTACAACTACATTCTAGTTGTTGGCGAGGAGGAAGTCAAAACTGGACAGGTGAGCGTACGGGTTAGAGATAAGGGAGCTTCCACAGTAATGAACATGGATAGCCTACTCAAACAATTCAAGGACAAAGTTGAAGCTTTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04066 ko05010 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230 map04066 map05010
Pfam Domains
Protein Families
Gp_dh_C Gp_dh_N

Protein Analysis

671

Amino Acids

77.0

Weight (kDa)

6.95

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGS PF02824 36 - 94 1.4e-16 TGS domain
tRNA_SAD PF07973 204 - 253 1.4e-13 Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b PF00587 352 - 564 1e-46 tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon PF03129 576 - 665 2.1e-20 Anticodon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018969)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0174381
rosa_multiflora Rmu_sc0003314.1_g000002 Rmu_sc0010418.1_g000004
rosa_roxburghii Rroxscaffold_3G00260290
rosa_rugosa Rorug07G0025100
rosa_samantha Rh7AG152700 Rh7BG154200 Rh7CG157800 Rh7DG153600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
Acc36I ACCTGC 1 cut(s) 1576
AccB1I GGYRCC 2 cut(s) 911, 1621
AccI GTMKAC 3 cut(s) 618, 714, 1323
AciI CCGC 1 cut(s) 656
AclWI GGATC 2 cut(s) 86, 540
AcoI YGGCCR 1 cut(s) 1703
AcuI CTGAAG 2 cut(s) 290, 1373
AcyI GRCGYC 2 cut(s) 194, 1299
AfaI GTAC 7 cut(s) 228, 847, 998, 1379, 1851, 1874, 1929
AfiI CCNNNNNNNGG 4 cut(s) 76, 254, 808, 1747
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 9 cut(s) 445, 460, 470, 521, 568, 575, 1090, 1990, 2003
AhlI ACTAGT 1 cut(s) 392
AjiI CACGTC 1 cut(s) 1724
AjnI CCWGG 4 cut(s) 51, 75, 883, 1451
AjuI GAANNNNNNNTTGG 2 cut(s) 1365, 1397
Alw21I GWGCWC 4 cut(s) 9, 861, 1210, 1689
Alw26I GTCTC 1 cut(s) 1750
Alw44I GTGCAC 1 cut(s) 1206
AlwI GGATC 2 cut(s) 86, 540
Ama87I CYCGRG 1 cut(s) 1106
AoxI GGCC 8 cut(s) 45, 78, 245, 298, 495, 1197, 1367, 1703
ApaLI GTGCAC 1 cut(s) 1206
ApeKI GCWGC 4 cut(s) 61, 479, 1031, 1415
Asp700I GAANNNNTTC 2 cut(s) 275, 2007
AspLEI GCGC 3 cut(s) 30, 326, 516
AspS9I GGNCC 6 cut(s) 79, 380, 653, 1487, 1777, 1833
AsuC2I CCSGG 5 cut(s) 302, 916, 1107, 1108, 1781
AsuHPI GGTGA 9 cut(s) 139, 147, 160, 229, 269, 443, 863, 1613, 1933
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 1106
AvaII GGWCC 5 cut(s) 380, 653, 1487, 1777, 1833
BaeGI GKGCMC 1 cut(s) 1210
BaeI ACNNNNGTAYC 2 cut(s) 592, 625
BanI GGYRCC 2 cut(s) 911, 1621
BanII GRGCYC 1 cut(s) 861
BauI CACGAG 1 cut(s) 1224
Bbv12I GWGCWC 4 cut(s) 9, 861, 1210, 1689
BbvCI CCTCAGC 2 cut(s) 213, 1410
BbvI GCAGC 4 cut(s) 73, 491, 1018, 1427
BccI CCATC 3 cut(s) 56, 1466, 1858
BceAI ACGGC 1 cut(s) 1690
BcgI CGANNNNNNTGC 2 cut(s) 1118, 1152
BciT130I CCWGG 4 cut(s) 53, 77, 885, 1453
BciVI GTATCC 1 cut(s) 962
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 5 cut(s) 302, 916, 1107, 1108, 1781
BcoDI GTCTC 1 cut(s) 1750
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 3 cut(s) 321, 393, 1886
BfmI CTRYAG 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 1576
BfuI GTATCC 1 cut(s) 962
BglI GCCNNNNNGGC 2 cut(s) 53, 1711
BglII AGATCT 1 cut(s) 1788
BisI GCNGC 4 cut(s) 62, 480, 1032, 1416
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 4 cut(s) 63, 481, 1033, 1417
Bme1390I CCNGG 9 cut(s) 53, 77, 302, 885, 916, 1107, 1108, 1453, 1781
Bme18I GGWCC 5 cut(s) 380, 653, 1487, 1777, 1833
BmeT110I CYCGRG 1 cut(s) 1106
BmgBI CACGTC 1 cut(s) 1724
BmgT120I GGNCC 6 cut(s) 79, 380, 653, 1487, 1777, 1833
BmiI GGNNCC 4 cut(s) 382, 631, 913, 1623
BmrFI CCNGG 9 cut(s) 53, 77, 302, 885, 916, 1107, 1108, 1453, 1781
BmsI GCATC 6 cut(s) 39, 409, 1195, 1240, 1417, 1504
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 2 cut(s) 739, 785
Bpu10I CCTNAGC 2 cut(s) 213, 1410
Bpu1102I GCTNAGC 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 3 cut(s) 8, 368, 563
BpuMI CCSGG 5 cut(s) 302, 916, 1107, 1108, 1781
BsaBI GATNNNNATC 1 cut(s) 1499
BsaHI GRCGYC 2 cut(s) 194, 1299
BsaJI CCNNGG 5 cut(s) 483, 633, 1106, 1381, 1451
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 254, 808, 1747
Bse1I ACTGG 6 cut(s) 722, 1170, 1200, 1219, 1315, 1918
Bse8I GATNNNNATC 1 cut(s) 1499
BseBI CCWGG 4 cut(s) 53, 77, 885, 1453
BseDI CCNNGG 5 cut(s) 483, 633, 1106, 1381, 1451
BseGI GGATG 5 cut(s) 48, 368, 1252, 1311, 1477
BseJI GATNNNNATC 1 cut(s) 1499
BseLI CCNNNNNNNGG 4 cut(s) 76, 254, 808, 1747
BseMII CTCAG 6 cut(s) 48, 204, 1401, 1605, 1874, 1883
BseNI ACTGG 6 cut(s) 722, 1170, 1200, 1219, 1315, 1918
BseRI GAGGAG 1 cut(s) 1913
BseSI GKGCMC 1 cut(s) 1210
BseXI GCAGC 4 cut(s) 73, 491, 1018, 1427
BseYI CCCAGC 1 cut(s) 1758
BshFI GGCC 8 cut(s) 47, 80, 247, 300, 497, 1199, 1369, 1705
BshNI GGYRCC 2 cut(s) 911, 1621
BsiHKAI GWGCWC 4 cut(s) 9, 861, 1210, 1689
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 5 cut(s) 116, 301, 916, 1107, 1780
BsiWI CGTACG 1 cut(s) 1927
BslFI GGGAC 5 cut(s) 248, 317, 366, 1089, 1796
BslI CCNNNNNNNGG 4 cut(s) 76, 254, 808, 1747
BsmAI GTCTC 1 cut(s) 1750
BsmFI GGGAC 5 cut(s) 248, 317, 366, 1089, 1796
BsmI GAATGC 4 cut(s) 205, 316, 516, 706
BsnI GGCC 8 cut(s) 47, 80, 247, 300, 497, 1199, 1369, 1705
BsoBI CYCGRG 1 cut(s) 1106
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 4 cut(s) 9, 861, 1210, 1689
Bsp1407I TGTACA 1 cut(s) 996
Bsp143I GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 1 cut(s) 656
BspANI GGCC 8 cut(s) 47, 80, 247, 300, 497, 1199, 1369, 1705
BspCNI CTCAG 6 cut(s) 49, 205, 1402, 1604, 1873, 1882
BspHI TCATGA 1 cut(s) 99
BspLI GGNNCC 4 cut(s) 382, 631, 913, 1623
BspMI ACCTGC 1 cut(s) 1576
BspPI GGATC 2 cut(s) 86, 540
BspQI GCTCTTC 3 cut(s) 44, 179, 866
BspT104I TTCGAA 1 cut(s) 182
BspT107I GGYRCC 2 cut(s) 911, 1621
BsrGI TGTACA 1 cut(s) 996
BsrI ACTGG 6 cut(s) 722, 1170, 1200, 1219, 1315, 1918
BssECI CCNNGG 5 cut(s) 483, 633, 1106, 1381, 1451
BssMI GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
BssNI GRCGYC 2 cut(s) 194, 1299
BssSI CACGAG 1 cut(s) 1224
BssT1I CCWWGG 3 cut(s) 483, 633, 1381
Bst2BI CACGAG 1 cut(s) 1224
Bst2UI CCWGG 4 cut(s) 53, 77, 885, 1453
Bst4CI ACNGT 6 cut(s) 231, 263, 290, 616, 737, 1957
Bst6I CTCTTC 5 cut(s) 44, 179, 396, 866, 1164
BstACI GRCGYC 2 cut(s) 194, 1299
BstAUI TGTACA 1 cut(s) 996
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 3 cut(s) 66, 245, 1573
BstDEI CTNAG 9 cut(s) 57, 213, 878, 1303, 1410, 1591, 1715, 1860, 1869
BstF5I GGATG 5 cut(s) 48, 368, 1252, 1311, 1477
BstHHI GCGC 3 cut(s) 30, 326, 516
BstKTI GATC 6 cut(s) 94, 535, 589, 832, 1351, 1791
BstMAI GTCTC 1 cut(s) 1750
BstMBI GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
BstNI CCWGG 4 cut(s) 53, 77, 885, 1453
BstNSI RCATGY 2 cut(s) 696, 997
BstSCI CCNGG 9 cut(s) 51, 75, 300, 883, 914, 1105, 1106, 1451, 1779
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 1 cut(s) 1210
BstV1I GCAGC 4 cut(s) 73, 491, 1018, 1427
BstX2I RGATCY 1 cut(s) 1788
BstXI CCANNNNNNTGG 1 cut(s) 891
BstYI RGATCY 1 cut(s) 1788
BsuI GTATCC 1 cut(s) 962
BsuRI GGCC 8 cut(s) 47, 80, 247, 300, 497, 1199, 1369, 1705
BtrI CACGTC 1 cut(s) 1724
BtsCI GGATG 5 cut(s) 48, 368, 1252, 1311, 1477
BtsIMutI CAGTG 5 cut(s) 295, 1207, 1212, 1510, 1541
BveI ACCTGC 1 cut(s) 1576
Cac8I GCNNGC 3 cut(s) 66, 245, 1573
CciI TCATGA 1 cut(s) 99
CfoI GCGC 3 cut(s) 30, 326, 516
Cfr13I GGNCC 6 cut(s) 79, 380, 653, 1487, 1777, 1833
Cfr9I CCCGGG 1 cut(s) 1106
CseI GACGC 2 cut(s) 183, 1288
Csp6I GTAC 7 cut(s) 227, 846, 997, 1378, 1850, 1873, 1928
CviAII CATG 7 cut(s) 100, 238, 693, 994, 1028, 1398, 1966
CviQI GTAC 7 cut(s) 227, 846, 997, 1378, 1850, 1873, 1928
DdeI CTNAG 9 cut(s) 57, 213, 878, 1303, 1410, 1591, 1715, 1860, 1869
DpnI GATC 6 cut(s) 93, 534, 588, 831, 1350, 1790
DpnII GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
DraI TTTAAA 1 cut(s) 945
EaeI YGGCCR 1 cut(s) 1703
Eam1104I CTCTTC 5 cut(s) 44, 179, 396, 866, 1164
EarI CTCTTC 5 cut(s) 44, 179, 396, 866, 1164
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 3 cut(s) 483, 633, 1381
Eco147I AGGCCT 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 861
Eco32I GATATC 1 cut(s) 1501
Eco47I GGWCC 5 cut(s) 380, 653, 1487, 1777, 1833
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 2 cut(s) 290, 1373
Eco88I CYCGRG 1 cut(s) 1106
EcoICRI GAGCTC 1 cut(s) 859
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 4 cut(s) 51, 75, 883, 1451
EcoRV GATATC 1 cut(s) 1501
EcoT14I CCWWGG 3 cut(s) 483, 633, 1381
EcoT22I ATGCAT 3 cut(s) 424, 1432, 1519
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 3 cut(s) 483, 633, 1381
FaeI CATG 7 cut(s) 103, 241, 696, 997, 1031, 1401, 1969
FalI AAGNNNNNCTT 2 cut(s) 1286, 1318
FaqI GGGAC 5 cut(s) 248, 317, 366, 1089, 1796
FatI CATG 7 cut(s) 99, 237, 692, 993, 1027, 1397, 1965
FbaI TGATCA 1 cut(s) 829
FblI GTMKAC 3 cut(s) 618, 714, 1323
Fnu4HI GCNGC 4 cut(s) 62, 480, 1032, 1416
FokI GGATG 5 cut(s) 35, 375, 1259, 1298, 1484
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 4 cut(s) 62, 480, 1032, 1416
FspBI CTAG 3 cut(s) 321, 393, 1886
GlaI GCGC 3 cut(s) 29, 325, 515
GluI GCNGC 4 cut(s) 62, 480, 1032, 1416
GsaI CCCAGC 1 cut(s) 1762
GsuI CTGGAG 2 cut(s) 739, 785
HaeIII GGCC 8 cut(s) 47, 80, 247, 300, 497, 1199, 1369, 1705
HapII CCGG 5 cut(s) 116, 301, 916, 1107, 1780
HgaI GACGC 2 cut(s) 183, 1288
HhaI GCGC 3 cut(s) 30, 326, 516
Hin1I GRCGYC 2 cut(s) 194, 1299
Hin1II CATG 7 cut(s) 103, 241, 696, 997, 1031, 1401, 1969
Hin6I GCGC 3 cut(s) 28, 324, 514
HinP1I GCGC 3 cut(s) 28, 324, 514
HincII GTYRAC 1 cut(s) 1819
HindII GTYRAC 1 cut(s) 1819
HindIII AAGCTT 3 cut(s) 742, 929, 2004
HinfI GANTC 5 cut(s) 470, 564, 949, 1236, 1271
HpaII CCGG 5 cut(s) 116, 301, 916, 1107, 1780
HphI GGTGA 9 cut(s) 139, 147, 160, 229, 269, 443, 863, 1613, 1933
Hpy166II GTNNAC 9 cut(s) 279, 368, 619, 653, 715, 1208, 1324, 1721, 1819
Hpy188III TCNNGA 2 cut(s) 100, 773
Hpy8I GTNNAC 9 cut(s) 279, 368, 619, 653, 715, 1208, 1324, 1721, 1819
Hpy99I CGWCG 3 cut(s) 167, 199, 810
HpyAV CCTTC 6 cut(s) 96, 118, 124, 166, 290, 1601
HpyCH4III ACNGT 6 cut(s) 231, 263, 290, 616, 737, 1957
HpyCH4IV ACGT 2 cut(s) 165, 1723
HpyF3I CTNAG 9 cut(s) 57, 213, 878, 1303, 1410, 1591, 1715, 1860, 1869
HpySE526I ACGT 2 cut(s) 165, 1723
Hsp92I GRCGYC 2 cut(s) 194, 1299
Hsp92II CATG 7 cut(s) 103, 241, 696, 997, 1031, 1401, 1969
HspAI GCGC 3 cut(s) 28, 324, 514
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
LguI GCTCTTC 3 cut(s) 44, 179, 866
LmnI GCTCC 4 cut(s) 635, 856, 1684, 1946
Lsp1109I GCAGC 4 cut(s) 73, 491, 1018, 1427
LweI GCATC 6 cut(s) 39, 409, 1195, 1240, 1417, 1504
MaeI CTAG 3 cut(s) 321, 393, 1886
MaeII ACGT 2 cut(s) 165, 1723
MaeIII GTNAC 4 cut(s) 148, 257, 869, 1272
MalI GATC 6 cut(s) 93, 534, 588, 831, 1350, 1790
MboI GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
MflI RGATCY 1 cut(s) 1788
MhlI GDGCHC 4 cut(s) 9, 861, 1210, 1689
MluCI AATT 5 cut(s) 18, 465, 1036, 1099, 1985
MlyI GAGTC 1 cut(s) 1280
Mph1103I ATGCAT 3 cut(s) 424, 1432, 1519
MroXI GAANNNNTTC 2 cut(s) 275, 2007
MseI TTAA 7 cut(s) 492, 944, 1118, 1220, 1293, 1433, 1520
MslI CAYNNNNRTG 3 cut(s) 691, 1191, 1958
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 5 cut(s) 116, 301, 916, 1107, 1780
MspR9I CCNGG 9 cut(s) 53, 77, 302, 885, 916, 1107, 1108, 1453, 1781
Mva1269I GAATGC 4 cut(s) 205, 316, 516, 706
MvaI CCWGG 4 cut(s) 53, 77, 885, 1453
NciI CCSGG 5 cut(s) 302, 916, 1107, 1108, 1781
NdeII GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
NlaIII CATG 7 cut(s) 103, 241, 696, 997, 1031, 1401, 1969
NlaIV GGNNCC 4 cut(s) 382, 631, 913, 1623
NmuCI GTSAC 4 cut(s) 148, 257, 869, 1272
NsiI ATGCAT 3 cut(s) 424, 1432, 1519
NspI RCATGY 2 cut(s) 696, 997
NspV TTCGAA 1 cut(s) 182
PagI TCATGA 1 cut(s) 99
PceI AGGCCT 1 cut(s) 247
PciI ACATGT 1 cut(s) 993
PciSI GCTCTTC 3 cut(s) 44, 179, 866
PctI GAATGC 4 cut(s) 205, 316, 516, 706
PdmI GAANNNNTTC 2 cut(s) 275, 2007
PfeI GAWTC 4 cut(s) 470, 564, 949, 1236
Pfl23II CGTACG 1 cut(s) 1927
PfoI TCCNGGA 2 cut(s) 883, 1779
PkrI GCNGC 4 cut(s) 63, 481, 1033, 1417
PleI GAGTC 1 cut(s) 1279
PpsI GAGTC 1 cut(s) 1279
PscI ACATGT 1 cut(s) 993
PsiI TTATAA 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 4 cut(s) 51, 75, 883, 1451
PspFI CCCAGC 1 cut(s) 1758
PspGI CCWGG 4 cut(s) 51, 75, 883, 1451
PspLI CGTACG 1 cut(s) 1927
PspN4I GGNNCC 4 cut(s) 382, 631, 913, 1623
PspPI GGNCC 6 cut(s) 79, 380, 653, 1487, 1777, 1833
PsuI RGATCY 1 cut(s) 1788
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 7 cut(s) 228, 847, 998, 1379, 1851, 1874, 1929
RsaNI GTAC 7 cut(s) 227, 846, 997, 1378, 1850, 1873, 1928
RseI CAYNNNNRTG 3 cut(s) 691, 1191, 1958
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 3 cut(s) 44, 179, 866
SaqAI TTAA 7 cut(s) 492, 944, 1118, 1220, 1293, 1433, 1520
SatI GCNGC 4 cut(s) 62, 480, 1032, 1416
Sau3AI GATC 6 cut(s) 91, 532, 586, 829, 1348, 1788
Sau96I GGNCC 6 cut(s) 79, 380, 653, 1487, 1777, 1833
SchI GAGTC 1 cut(s) 1280
ScrFI CCNGG 9 cut(s) 53, 77, 302, 885, 916, 1107, 1108, 1453, 1781
SduI GDGCHC 4 cut(s) 9, 861, 1210, 1689
SfaNI GCATC 6 cut(s) 39, 409, 1195, 1240, 1417, 1504
SfcI CTRYAG 1 cut(s) 619
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 5 cut(s) 380, 653, 1487, 1777, 1833
SmaI CCCGGG 1 cut(s) 1108
SmiMI CAYNNNNRTG 3 cut(s) 691, 1191, 1958
SmlI CTYRAG 3 cut(s) 23, 347, 542
SmoI CTYRAG 3 cut(s) 23, 347, 542
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 5 cut(s) 18, 465, 1036, 1099, 1985
SseBI AGGCCT 1 cut(s) 247
SsiI CCGC 1 cut(s) 656
SspI AATATT 1 cut(s) 111
SspMI CTAG 3 cut(s) 321, 393, 1886
SstI GAGCTC 1 cut(s) 861
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 9 cut(s) 51, 75, 300, 883, 914, 1105, 1106, 1451, 1779
StyI CCWWGG 3 cut(s) 483, 633, 1381
TaaI ACNGT 6 cut(s) 231, 263, 290, 616, 737, 1957
TaiI ACGT 2 cut(s) 168, 1726
TaqI TCGA 7 cut(s) 9, 42, 162, 182, 197, 1162, 1659
TasI AATT 5 cut(s) 18, 465, 1036, 1099, 1985
TatI WGTACW 2 cut(s) 996, 1872
TfiI GAWTC 4 cut(s) 470, 564, 949, 1236
Tru1I TTAA 7 cut(s) 492, 944, 1118, 1220, 1293, 1433, 1520
Tru9I TTAA 7 cut(s) 492, 944, 1118, 1220, 1293, 1433, 1520
TscAI CASTG 5 cut(s) 295, 1207, 1219, 1510, 1541
TseFI GTSAC 4 cut(s) 148, 257, 869, 1272
TseI GCWGC 4 cut(s) 61, 479, 1031, 1415
Tsp45I GTSAC 4 cut(s) 148, 257, 869, 1272
TspDTI ATGAA 7 cut(s) 309, 465, 1112, 1300, 1302, 1976, 2000
TspGWI ACGGA 1 cut(s) 1500
TspMI CCCGGG 1 cut(s) 1106
TspRI CASTG 5 cut(s) 295, 1207, 1219, 1510, 1541
VneI GTGCAC 1 cut(s) 1206
VpaK11BI GGWCC 5 cut(s) 380, 653, 1487, 1777, 1833
XceI RCATGY 2 cut(s) 696, 997
XmaI CCCGGG 1 cut(s) 1106
XmiI GTMKAC 3 cut(s) 618, 714, 1323
XmnI GAANNNNTTC 2 cut(s) 275, 2007
XspI CTAG 3 cut(s) 321, 393, 1886
Zsp2I ATGCAT 3 cut(s) 424, 1432, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.