Rorug07G0113900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015484
Physical Location & Seq
Reverse (-)
8894900 .. 8899276
4377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0113900.1

Sequence Viewer

Length: 3051 bp
ATGATGGATGGGCGGCGTCATTCTGTTGATATACCTATCTCTAAAGCTCTTGTGGCTCTGCGGAGGGTGAGGTCTCTAAGGGATCCATCAACTAATCCTATGAGCAAATTCTCTTCTCCGGTTGAGAATGTTAATTGGGAAACGAATTCGGGTAATGATATCTCTATGCTATTCTTGAATACTTTCCAAGAAGGTGGTTCTGACAACCGTAGTTGTTTGAGGCCAAAGAATTCGGATTTGTATGGACAAAGAGAGGATTGCTTAGATGATTTTGAACTAAACTTGGGTTTAGAAAAGTCTAGATTGATCCTGCCTGAGAATTCAGAGTGGGTTGGAAGCACAGGGAGTCCTAGGAGCAATCAGGGTGATGAGTTTGATCTTTCAGGGTCAGATAAGGAAGAGGTTTTTAGAAATAAATCACTGAGTCAGAGATATAAGAGCAGTCATATGGATAAAGGATTGGCCATGACATGCGTCAAGCCTTTGGAGGATGTGGATTATGAAGAAGTTGTCAGATCATCATGTTTAGAAAGAGTAGATCAGATTGCATCAAAACGAAAATCACAGTGTAAAAATCGTGTTGATTTGTCTGGGGCAATTGGTCATCGTAGGAGTCGAACTGGCAGTCCATGCCCTTCAGCAGGTGATGCTCTTTCAAGCCATGGTACACCCATTTTTGCAAACGAAGAGGTTGGTATTGGAGATCATGATCATCCTGGTGGTGGAATAAGCTGCTGCTGGTCAAGAACCCCTAGATTCAGAGAAGCTAACATTTCTTTTGATGCTGATGACCATCCTTTGTTATACAAGAATGTAGATGATATTTCACTTTATGACCACAGAAGCTTGAAGCGCATAGGAAATGAAACCAATTCACAGTTAGAAAAGCCCAGGAGTCTGAGTCAGAAATTCAGGCCAGAGTCTTTCATCGACTTGGTGGGACAAAATGTTGTTGCAAGGTCTCTTTTGGGTGCTATCTCTAGAGGAAGAATAACCTCATTCTATCTTTTCCATGGTCCACAAGGCACAGGAAAGACATCAGCCTCAAGAATTTTTGCTGCAGCACTTAATTGTCTTTCACTGGAGGAGTATAGGCCGTGTGGTCTCTGCTGCGAGTGTAATGTGTACTTTTCTGGAAACAGCAGAGATATCAAGGAAATAGATTCTGTGAGAATCAACCGGAGAGACCGCGTTAGGTCTCTTATTAAGAATGCAGCTATGCCTCCAGTTTCTTCACGGTTTAAGGTTTTCATCATTGATGAGTGCCACTTGATGCGTGGGGAAACATGGGTGACTATTTTGAGTAGCATCGATAACCTTTCTCAACATGTTGTCTTTGTGATGATCACTCCTGATCTGGATAAGCTGCCGCGGAATGCAGTATCACGGTCCCAGAGATATCACTTTCCAAAGATTAAGGATGGTGATATTGCAAGCAAGTTGGGAAAAATCTGTGTTGAAGAGGGTCTTGAGTTTGATCAGGTTGCTTTGAACTTCATTGCTGCCAAATCAAATGGTTCACTTAGGGATGCAGAGATGATGCTTGATCAGCTAAGTTTGCTTGGTAAAAGAATCACAATGGGTCTGGCATACGAGCTTATTGGAGCTGTTTCTGATGATGAATTGCTTGATTTGCTAGATCTGGCATTGTCATCTGACACTTCTAATACAGTTATAAGGGCCAGGGAGTTGATGAGATCAAGGATTGATCCCATGCAACTTATATCGCAGTTGGCAAATCTTATTATGGACATCCTTGCTGGTGAGGAAGGTGGTTCTGAAGTCCAGAAAAGGTTCTCTCGTAGGCATACCTCTGAAATGGACTTCCAGAAACTGAGTCATGCATTGAAAATATTCTCTGAAACTGAGAAGCAATTAAGGGTATCCAAGAATCAAACAACATGGCTTACTGCAGCTCTATTGCAGCTAAGCTCTGCAGAATCATCCTCATTGGATGCTAGTGATACAAAGAGTTTGAGAAATACACAAGAGAGAGGGCAGAGCTTCAAGAATCTTGACCCTACTTCATGCAATGTTAACACATCAGACAAGTTGGGAATGCAGAAGGACATTGATGAACAGTTGGAATCCATATGGAAGAGAGCTATAGATTTATGCCAATCCAGGTCATTCAAGAACTTTTTGAGGAAACAAGGAAGATTGTCGTCTCTATTTGTGAATCAAGGTCTCGCTATAGCTGAATTGGAATTCTGCCACCCTGATTATGTATCCAAGGCTGAGAAGTCATGGAAAATCATTGCAAGTGCACTTCAATCTCTATTCGGTTGTAATGTGGAGATCAGGATCAATCTTGTACGTGCTTCCGAGTCAAAGTATGCAAACGTGAAGAAGCCATCTTTCAGGTTGTTCAGCTGTTCCCGCAGAATGCAACAAAAGTCACCATCTTTCTGTGAGCGCGGAAGTGGTTCAGACTACTCTGAGTACACATCGGAAAAGCCTATATTAAGTGATAGACCTATTTTACCTTGTTCTTGTGATTGTGCTTCTCAACTGCCACATAACTGCTGTGACAAAATGGTGGTTGTAAGTACATTGAGAAATAGTGAAGGAAACATACTGAGCACAAGAGCGGCATCATCCCGCAGATCATTTGAAGATGATACACCAAAGGCACCTGGATTAATGGTTGATTCTTCAAAGGAAGAGGGAAGCAACCAGGACTGCCAGGTTTTATCTTTTCATCAACCTGAACATCAACCAAGGTGTTTCCCGAGAACTCTGAGGATTCAAAAGAAGTTGTGTTCGGCAGATGCTAATCAGGTGACCTGTTGCACTAAACTACAAAACAAGTTTGCAATCCCAAGTAGGACATCTTTTGAAGCTTGTCTTTTGGGAAGTGATTCATATGCTTTCTGCAGCGGCTCTGACAATGGTAACAACACTGACAACTATAAAGAAGAGTTGAAGGAAAATTCAGGTGTGCTTTGTTGGAAAACCCCCACGCTTCCCTCAGGAAAGGATTGGCAGCTGAGGCACCAACAGCAAAATTCGAAACTAGTTGGGTGGGTTCTTCCATGTGCAACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

1016

Amino Acids

113.6

Weight (kDa)

8.04

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 315 - 472 2.3e-21 DNA polymerase III, delta subunit
AAA PF00004 335 - 466 1.9e-08 ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid PF22608 477 - 517 2.2e-07 DNA polymerase III clamp loader subunit, ATPase lid domain
RCF1-5-like_lid PF21960 480 - 516 6.9e-06 RCF1/5-like, AAA+ ATPase lid domain
DnaA_N-like_STI PF23007 688 - 771 3.8e-31 STICHEL, DnaA_N-like alpha-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35290
fragaria_vesca FvH4_5g21990
malus_domestica MD06G1033600.v1.1 MD16G1275100.v1.1
prunus_persica Prupe.5G037100_v2.0.a1
pyrus_communis pycom06g02770 pycom16g24500
rosa_chinensis RchiOBHm_Chr7g0209491
rosa_laevigata RLG00000003124
rosa_multiflora Rmu_sc0000243.1_g000003
rosa_roxburghii Rroxscaffold_3G00249320
rosa_rugosa Rorug07G0113900
rosa_samantha Rh7AG247900 Rh7CG263400 Rh7DG253800
rosa_wichuraiana Rw7G020920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1676
AarI CACCTGC 1 cut(s) 632
Acc36I ACCTGC 1 cut(s) 632
AccB1I GGYRCC 2 cut(s) 2632, 2994
AccBSI CCGCTC 1 cut(s) 2591
AccII CGCG 3 cut(s) 1191, 1372, 2418
AclWI GGATC 5 cut(s) 77, 90, 301, 1703, 2312
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 9 cut(s) 107, 145, 229, 319, 908, 1050, 2207, 2932, 3007
AcuI CTGAAG 2 cut(s) 621, 1800
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 5 cut(s) 667, 1127, 2316, 2444, 2551
AfiI CCNNNNNNNGG 2 cut(s) 641, 722
AflIII ACRYGT 1 cut(s) 1327
AhlI ACTAGT 1 cut(s) 3016
AjnI CCWGG 7 cut(s) 715, 890, 1682, 2123, 2635, 2676, 2685
AloI GAACNNNNNNTCC 2 cut(s) 610, 642
Alw21I GWGCWC 2 cut(s) 2269, 2585
Alw26I GTCTC 7 cut(s) 78, 966, 1109, 1179, 1203, 2172, 2192
Alw44I GTGCAC 1 cut(s) 2265
AlwI GGATC 5 cut(s) 77, 90, 301, 1703, 2312
AlwNI CAGNNNCTG 1 cut(s) 1834
Ama87I CYCGRG 1 cut(s) 2731
AoxI GGCC 5 cut(s) 221, 462, 914, 1094, 1680
ApaLI GTGCAC 1 cut(s) 2265
ApoI RAATTY 9 cut(s) 107, 145, 229, 319, 908, 1050, 2207, 2932, 3007
ArsI GACNNNNNNTTYG 2 cut(s) 610, 642
AseI ATTAAT 1 cut(s) 2642
Asp700I GAANNNNTTC 5 cut(s) 182, 1793, 1853, 2425, 2860
AspA2I CCTAGG 1 cut(s) 350
AspLEI GCGC 2 cut(s) 855, 2418
AspS9I GGNCC 3 cut(s) 1016, 1389, 1680
AsuHPI GGTGA 8 cut(s) 79, 377, 656, 1303, 1436, 1775, 2391, 2794
AsuII TTCGAA 1 cut(s) 3011
AvaI CYCGRG 1 cut(s) 2731
AvaII GGWCC 2 cut(s) 1016, 1389
AvrII CCTAGG 1 cut(s) 350
AxyI CCTNAGG 1 cut(s) 2971
BaeGI GKGCMC 1 cut(s) 2269
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 1 cut(s) 82
BanI GGYRCC 2 cut(s) 2632, 2994
BarI GAAGNNNNNNTAC 2 cut(s) 2559, 2591
Bbv12I GWGCWC 2 cut(s) 2269, 2585
BbvCI CCTCAGC 1 cut(s) 2990
BccI CCATC 6 cut(s) 2, 94, 801, 1415, 2362, 2410
BceAI ACGGC 1 cut(s) 1081
BciT130I CCWGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
BciVI GTATCC 2 cut(s) 1894, 2239
BclI TGATCA 4 cut(s) 709, 1344, 1477, 1546
BcoDI GTCTC 7 cut(s) 78, 966, 1109, 1179, 1203, 2172, 2192
BcuI ACTAGT 1 cut(s) 3016
BfaI CTAG 7 cut(s) 300, 351, 753, 981, 1637, 1959, 3017
BfmI CTRYAG 6 cut(s) 1059, 1911, 1935, 2106, 2193, 2875
BfuAI ACCTGC 1 cut(s) 632
BfuI GTATCC 2 cut(s) 1894, 2239
BglII AGATCT 1 cut(s) 1639
BlnI CCTAGG 1 cut(s) 350
BlpI GCTNAGC 1 cut(s) 1928
Bme1390I CCNGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
Bme18I GGWCC 2 cut(s) 1016, 1389
BmeT110I CYCGRG 1 cut(s) 2731
BmgT120I GGNCC 3 cut(s) 1016, 1389, 1680
BmiI GGNNCC 4 cut(s) 84, 1391, 2634, 2996
BmrFI CCNGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
BoxI GACNNNNGTC 1 cut(s) 473
BpmI CTGGAG 2 cut(s) 1103, 1209
Bpu10I CCTNAGC 1 cut(s) 2990
Bpu1102I GCTNAGC 1 cut(s) 1928
Bpu14I TTCGAA 1 cut(s) 3011
BpuEI CTTGAG 2 cut(s) 1030, 1490
Bsa29I ATCGAT 1 cut(s) 1311
BsaAI YACGTR 1 cut(s) 2318
BsaBI GATNNNNATC 4 cut(s) 708, 792, 2303, 2775
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 6 cut(s) 78, 966, 1109, 1179, 1203, 2192
BsaJI CCNNGG 8 cut(s) 350, 661, 890, 1012, 1370, 1683, 2232, 2720
BsaWI WCCGGW 2 cut(s) 118, 1179
BsaXI ACNNNNNCTCC 2 cut(s) 886, 916
Bsc4I CCNNNNNNNGG 2 cut(s) 641, 722
Bse1I ACTGG 3 cut(s) 625, 1086, 1226
Bse21I CCTNAGG 1 cut(s) 2971
Bse3DI GCAATG 3 cut(s) 1497, 2038, 2256
Bse8I GATNNNNATC 4 cut(s) 708, 792, 2303, 2775
BseBI CCWGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
BseCI ATCGAT 1 cut(s) 1311
BseDI CCNNGG 8 cut(s) 350, 661, 890, 1012, 1370, 1683, 2232, 2720
BseJI GATNNNNATC 4 cut(s) 708, 792, 2303, 2775
BseLI CCNNNNNNNGG 2 cut(s) 641, 722
BseMI GCAATG 3 cut(s) 1497, 2038, 2256
BseNI ACTGG 3 cut(s) 625, 1086, 1226
BseRI GAGGAG 1 cut(s) 1100
BseSI GKGCMC 1 cut(s) 2269
Bsh1236I CGCG 3 cut(s) 1191, 1372, 2418
BshFI GGCC 5 cut(s) 223, 464, 916, 1096, 1682
BshNI GGYRCC 2 cut(s) 2632, 2994
BshVI ATCGAT 1 cut(s) 1311
BsiHKAI GWGCWC 2 cut(s) 2269, 2585
BsiHKCI CYCGRG 1 cut(s) 2731
BsiSI CCGG 2 cut(s) 119, 1180
BslFI GGGAC 2 cut(s) 954, 1375
BslI CCNNNNNNNGG 2 cut(s) 641, 722
BsmAI GTCTC 7 cut(s) 78, 966, 1109, 1179, 1203, 2172, 2192
BsmBI CGTCTC 1 cut(s) 2172
BsmFI GGGAC 2 cut(s) 954, 1375
BsmI GAATGC 4 cut(s) 1216, 1381, 2064, 2391
BsnI GGCC 5 cut(s) 223, 464, 916, 1096, 1682
Bso31I GGTCTC 6 cut(s) 78, 966, 1109, 1179, 1203, 2192
BsoBI CYCGRG 1 cut(s) 2731
Bsp119I TTCGAA 1 cut(s) 3011
Bsp1286I GDGCHC 2 cut(s) 2269, 2585
Bsp1720I GCTNAGC 1 cut(s) 1928
Bsp19I CCATGG 2 cut(s) 661, 1012
BspANI GGCC 5 cut(s) 223, 464, 916, 1096, 1682
BspDI ATCGAT 1 cut(s) 1311
BspFNI CGCG 3 cut(s) 1191, 1372, 2418
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 4 cut(s) 84, 1391, 2634, 2996
BspMAI CTGCAG 4 cut(s) 1063, 1915, 1939, 2879
BspMI ACCTGC 1 cut(s) 632
BspPI GGATC 5 cut(s) 77, 90, 301, 1703, 2312
BspT104I TTCGAA 1 cut(s) 3011
BspT107I GGYRCC 2 cut(s) 2632, 2994
BspTNI GGTCTC 6 cut(s) 78, 966, 1109, 1179, 1203, 2192
BsrBI CCGCTC 1 cut(s) 2591
BsrDI GCAATG 3 cut(s) 1497, 2038, 2256
BsrI ACTGG 3 cut(s) 625, 1086, 1226
BssECI CCNNGG 8 cut(s) 350, 661, 890, 1012, 1370, 1683, 2232, 2720
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 5 cut(s) 350, 661, 1012, 2232, 2720
Bst2UI CCWGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
Bst4CI ACNGT 7 cut(s) 209, 567, 879, 1239, 1389, 1672, 2082
Bst6I CTCTTC 7 cut(s) 118, 393, 681, 1455, 2093, 2658, 2913
BstACI GRCGYC 1 cut(s) 16
BstAPI GCANNNNNTGC 2 cut(s) 630, 647
BstBAI YACGTR 1 cut(s) 2318
BstBI TTCGAA 1 cut(s) 3011
BstC8I GCNNGC 1 cut(s) 1435
BstDSI CCRYGG 3 cut(s) 661, 1012, 1370
BstEII GGTNACC 1 cut(s) 2782
BstENI CCTNNNNNAGG 1 cut(s) 639
BstFNI CGCG 3 cut(s) 1191, 1372, 2418
BstHHI GCGC 2 cut(s) 855, 2418
BstMAI GTCTC 7 cut(s) 78, 966, 1109, 1179, 1203, 2172, 2192
BstNI CCWGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
BstNSI RCATGY 2 cut(s) 474, 1331
BstPAI GACNNNNGTC 1 cut(s) 473
BstPI GGTNACC 1 cut(s) 2782
BstSCI CCNGG 7 cut(s) 715, 890, 1682, 2123, 2635, 2676, 2685
BstSFI CTRYAG 6 cut(s) 1059, 1911, 1935, 2106, 2193, 2875
BstSLI GKGCMC 1 cut(s) 2269
BstUI CGCG 3 cut(s) 1191, 1372, 2418
BstX2I RGATCY 2 cut(s) 82, 1639
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 2 cut(s) 82, 1639
Bsu15I ATCGAT 1 cut(s) 1311
Bsu36I CCTNAGG 1 cut(s) 2971
BsuI GTATCC 2 cut(s) 1894, 2239
BsuRI GGCC 5 cut(s) 223, 464, 916, 1096, 1682
BsuTUI ATCGAT 1 cut(s) 1311
BtgI CCRYGG 3 cut(s) 661, 1012, 1370
BtsIMutI CAGTG 4 cut(s) 419, 572, 1079, 2901
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 1 cut(s) 1435
CaiI CAGNNNCTG 1 cut(s) 1834
CciI TCATGA 1 cut(s) 706
CfoI GCGC 2 cut(s) 855, 2418
Cfr13I GGNCC 3 cut(s) 1016, 1389, 1680
Cfr42I CCGCGG 1 cut(s) 1373
ClaI ATCGAT 1 cut(s) 1311
CseI GACGC 2 cut(s) 5, 463
Csp6I GTAC 5 cut(s) 666, 1126, 2315, 2443, 2550
CviQI GTAC 5 cut(s) 666, 1126, 2315, 2443, 2550
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 7 cut(s) 118, 393, 681, 1455, 2093, 2658, 2913
EarI CTCTTC 7 cut(s) 118, 393, 681, 1455, 2093, 2658, 2913
Eco130I CCWWGG 5 cut(s) 350, 661, 1012, 2232, 2720
Eco31I GGTCTC 6 cut(s) 78, 966, 1109, 1179, 1203, 2192
Eco32I GATATC 3 cut(s) 160, 1150, 1400
Eco47I GGWCC 2 cut(s) 1016, 1389
Eco57I CTGAAG 2 cut(s) 621, 1800
Eco81I CCTNAGG 1 cut(s) 2971
Eco88I CYCGRG 1 cut(s) 2731
Eco91I GGTNACC 1 cut(s) 2782
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO65I GGTNACC 1 cut(s) 2782
EcoRI GAATTC 4 cut(s) 145, 229, 319, 2207
EcoRII CCWGG 7 cut(s) 715, 890, 1682, 2123, 2635, 2676, 2685
EcoRV GATATC 3 cut(s) 160, 1150, 1400
EcoT14I CCWWGG 5 cut(s) 350, 661, 1012, 2232, 2720
EcoT22I ATGCAT 1 cut(s) 1846
ErhI CCWWGG 5 cut(s) 350, 661, 1012, 2232, 2720
Esp3I CGTCTC 1 cut(s) 2172
FalI AAGNNNNNCTT 4 cut(s) 1452, 1484, 2832, 2864
FaqI GGGAC 2 cut(s) 954, 1375
FauI CCCGC 2 cut(s) 2387, 2609
FauNDI CATATG 3 cut(s) 447, 2093, 2866
FbaI TGATCA 4 cut(s) 709, 1344, 1477, 1546
FspBI CTAG 7 cut(s) 300, 351, 753, 981, 1637, 1959, 3017
GlaI GCGC 2 cut(s) 854, 2417
GsuI CTGGAG 2 cut(s) 1103, 1209
HaeIII GGCC 5 cut(s) 223, 464, 916, 1096, 1682
HapII CCGG 2 cut(s) 119, 1180
HgaI GACGC 2 cut(s) 5, 463
HhaI GCGC 2 cut(s) 855, 2418
Hin1I GRCGYC 1 cut(s) 16
Hin6I GCGC 2 cut(s) 853, 2416
HinP1I GCGC 2 cut(s) 853, 2416
HincII GTYRAC 1 cut(s) 2038
HindII GTYRAC 1 cut(s) 2038
HindIII AAGCTT 2 cut(s) 844, 2841
HpaI GTTAAC 1 cut(s) 2038
HpaII CCGG 2 cut(s) 119, 1180
HphI GGTGA 8 cut(s) 79, 377, 656, 1303, 1436, 1775, 2391, 2794
Hpy166II GTNNAC 7 cut(s) 668, 1019, 1126, 1520, 2038, 2267, 2445
Hpy8I GTNNAC 7 cut(s) 668, 1019, 1126, 1520, 2038, 2267, 2445
HpyAV CCTTC 6 cut(s) 185, 645, 1763, 2059, 2561, 2920
HpyCH4III ACNGT 7 cut(s) 209, 567, 879, 1239, 1389, 1672, 2082
HpyCH4IV ACGT 2 cut(s) 2317, 2343
HpySE526I ACGT 2 cut(s) 2317, 2343
Hsp92I GRCGYC 1 cut(s) 16
HspAI GCGC 2 cut(s) 853, 2416
Ksp22I TGATCA 4 cut(s) 709, 1344, 1477, 1546
KspAI GTTAAC 1 cut(s) 2038
KspI CCGCGG 1 cut(s) 1373
LmnI GCTCC 2 cut(s) 354, 1604
MaeI CTAG 7 cut(s) 300, 351, 753, 981, 1637, 1959, 3017
MaeII ACGT 2 cut(s) 2317, 2343
MaeIII GTNAC 5 cut(s) 1291, 2397, 2528, 2782, 2894
MbiI CCGCTC 1 cut(s) 2591
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 2 cut(s) 82, 1639
MhlI GDGCHC 2 cut(s) 2269, 2585
MlsI TGGCCA 1 cut(s) 464
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 8 cut(s) 355, 433, 622, 904, 910, 929, 1846, 2336
MmeI TCCRAC 3 cut(s) 313, 2064, 2930
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 1 cut(s) 1846
MroXI GAANNNNTTC 5 cut(s) 182, 1793, 1853, 2425, 2860
MscI TGGCCA 1 cut(s) 464
MseI TTAA 9 cut(s) 132, 1068, 1206, 1242, 1416, 1877, 2037, 2465, 2642
MslI CAYNNNNRTG 1 cut(s) 717
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 4 cut(s) 1372, 2373, 2880, 2989
MspI CCGG 2 cut(s) 119, 1180
MspR9I CCNGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
MunI CAATTG 1 cut(s) 597
Mva1269I GAATGC 4 cut(s) 1216, 1381, 2064, 2391
MvaI CCWGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
MvnI CGCG 3 cut(s) 1191, 1372, 2418
NcoI CCATGG 2 cut(s) 661, 1012
NdeI CATATG 3 cut(s) 447, 2093, 2866
NlaIV GGNNCC 4 cut(s) 84, 1391, 2634, 2996
NmuCI GTSAC 4 cut(s) 1291, 2397, 2528, 2782
NsiI ATGCAT 1 cut(s) 1846
NspI RCATGY 2 cut(s) 474, 1331
NspV TTCGAA 1 cut(s) 3011
PagI TCATGA 1 cut(s) 706
PaqCI CACCTGC 1 cut(s) 632
PciI ACATGT 1 cut(s) 1327
PcsI WCGNNNNNNNCGW 1 cut(s) 613
PctI GAATGC 4 cut(s) 1216, 1381, 2064, 2391
PdmI GAANNNNTTC 5 cut(s) 182, 1793, 1853, 2425, 2860
PleI GAGTC 8 cut(s) 354, 432, 621, 903, 909, 928, 1845, 2335
PpsI GAGTC 8 cut(s) 354, 432, 621, 903, 909, 928, 1845, 2335
Ppu21I YACGTR 1 cut(s) 2318
PscI ACATGT 1 cut(s) 1327
PshAI GACNNNNGTC 1 cut(s) 473
PshBI ATTAAT 1 cut(s) 2642
PsiI TTATAA 1 cut(s) 1676
Psp6I CCWGG 7 cut(s) 715, 890, 1682, 2123, 2635, 2676, 2685
PspEI GGTNACC 1 cut(s) 2782
PspGI CCWGG 7 cut(s) 715, 890, 1682, 2123, 2635, 2676, 2685
PspN4I GGNNCC 4 cut(s) 84, 1391, 2634, 2996
PspPI GGNCC 3 cut(s) 1016, 1389, 1680
PstI CTGCAG 4 cut(s) 1063, 1915, 1939, 2879
PstNI CAGNNNCTG 1 cut(s) 1834
PsuI RGATCY 2 cut(s) 82, 1639
PvuII CAGCTG 2 cut(s) 2373, 2989
RsaI GTAC 5 cut(s) 667, 1127, 2316, 2444, 2551
RsaNI GTAC 5 cut(s) 666, 1126, 2315, 2443, 2550
RseI CAYNNNNRTG 1 cut(s) 717
SacII CCGCGG 1 cut(s) 1373
SaqAI TTAA 9 cut(s) 132, 1068, 1206, 1242, 1416, 1877, 2037, 2465, 2642
Sau96I GGNCC 3 cut(s) 1016, 1389, 1680
SchI GAGTC 8 cut(s) 355, 433, 622, 904, 910, 929, 1846, 2336
ScrFI CCNGG 7 cut(s) 717, 892, 1684, 2125, 2637, 2678, 2687
SduI GDGCHC 2 cut(s) 2269, 2585
SfcI CTRYAG 6 cut(s) 1059, 1911, 1935, 2106, 2193, 2875
Sfr303I CCGCGG 1 cut(s) 1373
SfuI TTCGAA 1 cut(s) 3011
SgrBI CCGCGG 1 cut(s) 1373
SinI GGWCC 2 cut(s) 1016, 1389
SmiMI CAYNNNNRTG 1 cut(s) 717
SmlI CTYRAG 2 cut(s) 1045, 1469
SmoI CTYRAG 2 cut(s) 1045, 1469
SpeI ACTAGT 1 cut(s) 3016
SspI AATATT 1 cut(s) 1854
SspMI CTAG 7 cut(s) 300, 351, 753, 981, 1637, 1959, 3017
StyD4I CCNGG 7 cut(s) 715, 890, 1682, 2123, 2635, 2676, 2685
StyI CCWWGG 5 cut(s) 350, 661, 1012, 2232, 2720
TaaI ACNGT 7 cut(s) 209, 567, 879, 1239, 1389, 1672, 2082
TaiI ACGT 2 cut(s) 2320, 2346
TaqI TCGA 4 cut(s) 616, 930, 1311, 3011
TatI WGTACW 3 cut(s) 1125, 2442, 2549
TauI GCSGC 4 cut(s) 16, 1372, 2594, 2883
Tru1I TTAA 9 cut(s) 132, 1068, 1206, 1242, 1416, 1877, 2037, 2465, 2642
Tru9I TTAA 9 cut(s) 132, 1068, 1206, 1242, 1416, 1877, 2037, 2465, 2642
TscAI CASTG 4 cut(s) 426, 572, 1086, 2908
TseFI GTSAC 4 cut(s) 1291, 2397, 2528, 2782
Tsp45I GTSAC 4 cut(s) 1291, 2397, 2528, 2782
TspRI CASTG 4 cut(s) 426, 572, 1086, 2908
VneI GTGCAC 1 cut(s) 2265
VpaK11BI GGWCC 2 cut(s) 1016, 1389
VspI ATTAAT 1 cut(s) 2642
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 9 cut(s) 107, 145, 229, 319, 908, 1050, 2207, 2932, 3007
XbaI TCTAGA 2 cut(s) 299, 980
XceI RCATGY 2 cut(s) 474, 1331
XcmI CCANNNNNNNNNTGG 1 cut(s) 1274
XmaJI CCTAGG 1 cut(s) 350
XmnI GAANNNNTTC 5 cut(s) 182, 1793, 1853, 2425, 2860
XspI CTAG 7 cut(s) 300, 351, 753, 981, 1637, 1959, 3017
Zsp2I ATGCAT 1 cut(s) 1846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.