Rorug07G0206000

Mitotic-spindle organizing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008654
Physical Location & Seq
Forward (+)
18125821 .. 18127753
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0206000.1

Sequence Viewer

Length: 723 bp
ATGGCAACAGCGCCGGTGAAGCCGCCGCTGCACAACTTCCCGCTATCGTTCTTGAAATGGGGGAGCAAGAACCACACCAACACCAACCACCGCTACCGCCGCCCCGTCTCCGCCGAGCCCGAACCGTCCGACGATTCCGAGCCGCCGCAGCACCACCACCACGGCAATCGCGTCGGATCCCGCACCGCGCGCCACCGCTTCTCGCTCACTTCCTGCTCCGAGAAGCTTCCGGGGCCGCAGAATTCCGAGAAAGAGAGCGAGGACAGCGACGACGACGGTAAGGCCGCGGCCGCCACCGCCGCCGCGGCGGAGGAGGCCGAGGTTCAGAAGCCGTGGAATCTGAGGCCGAGGCGAGCACCGACGGCGATGACTTCGAAGGCGAATAATACTACTACTGCGAACGGCGAGCTGCATGAGCCGGAGGGTCCGAACCCGAACCCGAAGCAGGGCGAGCAGCCGGCGCCGAAGTCGATGAGGCTGAGGGGTTTGGCTGAAGGTCCGAGCATGGAGAAGAAGAAGGAGAAGAGGAAGTTCTGGATCGCTTTGTCTAAGGATGAAATTGAAGAGGACATATTCATCATGACCGGGTCCAGACCCGCCCGGCGGCCCAAGAAGCGGCCCAAGAATGTCCAGAAACAGCTCGATAATTGTTTTCCGGGTTTATGGCTCGTGGGGTTTACAGCTGATGCTTATCGGGGTGCCGATTCTCCGACGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000923 GO:0000930 GO:0000931 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005640 GO:0005694 GO:0005737 GO:0005815 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006338 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008104 GO:0008150 GO:0008274 GO:0009524 GO:0009574 GO:0009987 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0019867 GO:0022402 GO:0022607 GO:0031021 GO:0031055 GO:0031090 GO:0031109 GO:0031497 GO:0031503 GO:0031965 GO:0031967 GO:0031968 GO:0031975 GO:0032153 GO:0032155 GO:0032991 GO:0033036 GO:0033566 GO:0034080 GO:0034508 GO:0034613 GO:0034622 GO:0034629 GO:0034724 GO:0034728 GO:0042175 GO:0042393 GO:0043015 GO:0043044 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043486 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0046785 GO:0048285 GO:0051179 GO:0051225 GO:0051258 GO:0051276 GO:0051415 GO:0051418 GO:0051641 GO:0061641 GO:0065003 GO:0065004 GO:0070727 GO:0070925 GO:0071103 GO:0071824 GO:0071840 GO:0072686 GO:0090307 GO:0097435 GO:0098588 GO:0098687 GO:0098805 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MOZART1

Protein Analysis

240

Amino Acids

26.72

Weight (kDa)

9.56

Isoelectric Point (pI)

55.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 182 - 231 4.8e-26 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 460, 698
AccII CGCG 5 cut(s) 171, 188, 190, 287, 305
AclWI GGATC 3 cut(s) 171, 184, 545
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 513
AcyI GRCGYC 1 cut(s) 461
AfiI CCNNNNNNNGG 4 cut(s) 445, 446, 603, 615
AgsI TTSAA 2 cut(s) 55, 563
AluBI AGCT 4 cut(s) 226, 409, 640, 683
AluI AGCT 4 cut(s) 226, 409, 640, 683
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 3 cut(s) 171, 184, 545
AoxI GGCC 7 cut(s) 233, 282, 288, 315, 344, 605, 617
ApeKI GCWGC 4 cut(s) 28, 148, 409, 454
ApoI RAATTY 1 cut(s) 241
AspLEI GCGC 4 cut(s) 13, 190, 192, 463
AspS9I GGNCC 6 cut(s) 233, 425, 497, 588, 606, 618
AsuC2I CCSGG 4 cut(s) 231, 586, 601, 657
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 374
AvaII GGWCC 3 cut(s) 425, 497, 588
BamHI GGATCC 1 cut(s) 176
BanI GGYRCC 2 cut(s) 460, 698
BanII GRGCYC 1 cut(s) 120
BauI CACGAG 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 479
BbvI GCAGC 4 cut(s) 15, 160, 396, 466
BceAI ACGGC 4 cut(s) 178, 316, 378, 418
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BcnI CCSGG 4 cut(s) 231, 586, 601, 657
BcoDI GTCTC 1 cut(s) 112
BfoI RGCGCY 2 cut(s) 14, 464
BglI GCCNNNNNGGC 1 cut(s) 305
Bme1390I CCNGG 4 cut(s) 231, 586, 601, 657
Bme18I GGWCC 3 cut(s) 425, 497, 588
BmgT120I GGNCC 6 cut(s) 233, 425, 497, 588, 606, 618
BmiI GGNNCC 6 cut(s) 178, 234, 426, 462, 589, 700
BmrFI CCNGG 4 cut(s) 231, 586, 601, 657
BmsI GCATC 1 cut(s) 676
Bpu10I CCTNAGC 1 cut(s) 479
Bpu14I TTCGAA 1 cut(s) 374
BpuMI CCSGG 4 cut(s) 231, 586, 601, 657
BsaBI GATNNNNATC 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 461
BsaJI CCNNGG 7 cut(s) 160, 230, 285, 303, 318, 332, 347
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 446, 603, 615
Bse118I RCCGGY 2 cut(s) 13, 457
Bse8I GATNNNNATC 1 cut(s) 690
BseDI CCNNGG 7 cut(s) 160, 230, 285, 303, 318, 332, 347
BseGI GGATG 1 cut(s) 559
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 4 cut(s) 445, 446, 603, 615
BseMII CTCAG 2 cut(s) 332, 470
BsePI GCGCGC 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 326
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 4 cut(s) 15, 160, 396, 466
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 5 cut(s) 171, 188, 190, 287, 305
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 7 cut(s) 235, 284, 290, 317, 346, 607, 619
BshNI GGYRCC 2 cut(s) 460, 698
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 7 cut(s) 14, 230, 419, 458, 585, 601, 656
BslI CCNNNNNNNGG 4 cut(s) 445, 446, 603, 615
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
BsnI GGCC 7 cut(s) 235, 284, 290, 317, 346, 607, 619
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 120, 358
Bsp143I GATC 2 cut(s) 176, 537
BspANI GGCC 7 cut(s) 235, 284, 290, 317, 346, 607, 619
BspCNI CTCAG 2 cut(s) 333, 471
BspFNI CGCG 5 cut(s) 171, 188, 190, 287, 305
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 6 cut(s) 178, 234, 426, 462, 589, 700
BspPI GGATC 3 cut(s) 171, 184, 545
BspT104I TTCGAA 1 cut(s) 374
BspT107I GGYRCC 2 cut(s) 460, 698
BsrFI RCCGGY 2 cut(s) 13, 457
BssAI RCCGGY 2 cut(s) 13, 457
BssECI CCNNGG 7 cut(s) 160, 230, 285, 303, 318, 332, 347
BssHII GCGCGC 1 cut(s) 188
BssMI GATC 2 cut(s) 176, 537
BssNI GRCGYC 1 cut(s) 461
BssSI CACGAG 1 cut(s) 668
Bst2BI CACGAG 1 cut(s) 668
Bst4CI ACNGT 2 cut(s) 126, 278
Bst6I CTCTTC 2 cut(s) 518, 558
BstACI GRCGYC 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 5 cut(s) 190, 354, 407, 452, 459
BstDEI CTNAG 3 cut(s) 341, 479, 549
BstDSI CCRYGG 4 cut(s) 160, 285, 303, 332
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 5 cut(s) 171, 188, 190, 287, 305
BstH2I RGCGCY 2 cut(s) 14, 464
BstHHI GCGC 4 cut(s) 13, 190, 192, 463
BstKTI GATC 2 cut(s) 179, 540
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 176, 537
BstMCI CGRYCG 1 cut(s) 291
BstSCI CCNGG 4 cut(s) 229, 584, 599, 655
BstUI CGCG 5 cut(s) 171, 188, 190, 287, 305
BstV1I GCAGC 4 cut(s) 15, 160, 396, 466
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BstZI CGGCCG 1 cut(s) 288
BsuRI GGCC 7 cut(s) 235, 284, 290, 317, 346, 607, 619
BtgI CCRYGG 4 cut(s) 160, 285, 303, 332
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 1 cut(s) 559
Cac8I GCNNGC 5 cut(s) 190, 354, 407, 452, 459
CciI TCATGA 1 cut(s) 579
CciNI GCGGCCGC 1 cut(s) 288
CfoI GCGC 4 cut(s) 13, 190, 192, 463
Cfr10I RCCGGY 2 cut(s) 13, 457
Cfr13I GGNCC 6 cut(s) 233, 425, 497, 588, 606, 618
Cfr42I CCGCGG 2 cut(s) 288, 306
CseI GACGC 1 cut(s) 160
CviAII CATG 3 cut(s) 413, 505, 580
DdeI CTNAG 3 cut(s) 341, 479, 549
DinI GGCGCC 1 cut(s) 462
DpnI GATC 2 cut(s) 178, 539
DpnII GATC 2 cut(s) 176, 537
EaeI YGGCCR 1 cut(s) 288
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 518, 558
EarI CTCTTC 2 cut(s) 518, 558
EciI GGCGGA 2 cut(s) 100, 323
EclXI CGGCCG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 3 cut(s) 425, 497, 588
Eco52I CGGCCG 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 120
EgeI GGCGCC 1 cut(s) 462
EheI GGCGCC 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 3 cut(s) 416, 508, 583
FaiI YATR 5 cut(s) 414, 506, 572, 581, 664
FatI CATG 3 cut(s) 412, 504, 579
FauI CCCGC 3 cut(s) 48, 188, 604
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 120
GlaI GCGC 4 cut(s) 12, 189, 191, 462
HaeII RGCGCY 2 cut(s) 14, 464
HaeIII GGCC 7 cut(s) 235, 284, 290, 317, 346, 607, 619
HapII CCGG 7 cut(s) 14, 230, 419, 458, 585, 601, 656
HgaI GACGC 1 cut(s) 160
HhaI GCGC 4 cut(s) 13, 190, 192, 463
Hin1I GRCGYC 1 cut(s) 461
Hin1II CATG 3 cut(s) 416, 508, 583
Hin6I GCGC 4 cut(s) 11, 188, 190, 461
HinP1I GCGC 4 cut(s) 11, 188, 190, 461
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 3 cut(s) 134, 337, 704
HpaII CCGG 7 cut(s) 14, 230, 419, 458, 585, 601, 656
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 678
Hpy188III TCNNGA 5 cut(s) 52, 535, 580, 591, 631
Hpy8I GTNNAC 1 cut(s) 678
Hpy99I CGWCG 7 cut(s) 134, 176, 272, 275, 278, 364, 715
HpyAV CCTTC 3 cut(s) 370, 488, 511
HpyCH4III ACNGT 2 cut(s) 126, 278
HpyCH4V TGCA 2 cut(s) 31, 412
HpyF3I CTNAG 3 cut(s) 341, 479, 549
Hsp92I GRCGYC 1 cut(s) 461
Hsp92II CATG 3 cut(s) 416, 508, 583
HspAI GCGC 4 cut(s) 11, 188, 190, 461
KasI GGCGCC 1 cut(s) 460
KroI GCCGGC 1 cut(s) 457
KroNI GCCGGC 1 cut(s) 459
KspI CCGCGG 2 cut(s) 288, 306
Kzo9I GATC 2 cut(s) 176, 537
LmnI GCTCC 2 cut(s) 63, 221
Lsp1109I GCAGC 4 cut(s) 15, 160, 396, 466
LweI GCATC 1 cut(s) 676
MalI GATC 2 cut(s) 178, 539
MboI GATC 2 cut(s) 176, 537
MboII GAAGA 4 cut(s) 523, 526, 535, 575
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 2 cut(s) 120, 358
MluCI AATT 3 cut(s) 241, 558, 646
Mly113I GGCGCC 1 cut(s) 461
MmeI TCCRAC 2 cut(s) 153, 154
MroNI GCCGGC 1 cut(s) 457
MspA1I CMGCKG 4 cut(s) 28, 287, 305, 683
MspI CCGG 7 cut(s) 14, 230, 419, 458, 585, 601, 656
MspR9I CCNGG 4 cut(s) 231, 586, 601, 657
MvnI CGCG 5 cut(s) 171, 188, 190, 287, 305
NaeI GCCGGC 1 cut(s) 459
NarI GGCGCC 1 cut(s) 461
NciI CCSGG 4 cut(s) 231, 586, 601, 657
NdeII GATC 2 cut(s) 176, 537
NgoMIV GCCGGC 1 cut(s) 457
NlaIII CATG 3 cut(s) 416, 508, 583
NlaIV GGNNCC 6 cut(s) 178, 234, 426, 462, 589, 700
NmeAIII GCCGAG 3 cut(s) 139, 343, 372
NotI GCGGCCGC 1 cut(s) 288
NspV TTCGAA 1 cut(s) 374
PagI TCATGA 1 cut(s) 579
PauI GCGCGC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdiI GCCGGC 1 cut(s) 459
PfeI GAWTC 3 cut(s) 134, 337, 704
PflFI GACNNNGTC 1 cut(s) 586
PluTI GGCGCC 1 cut(s) 464
PspN4I GGNNCC 6 cut(s) 178, 234, 426, 462, 589, 700
PspPI GGNCC 6 cut(s) 233, 425, 497, 588, 606, 618
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 586
PteI GCGCGC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 683
SacII CCGCGG 2 cut(s) 288, 306
Sau3AI GATC 2 cut(s) 176, 537
Sau96I GGNCC 6 cut(s) 233, 425, 497, 588, 606, 618
ScrFI CCNGG 4 cut(s) 231, 586, 601, 657
SduI GDGCHC 2 cut(s) 120, 358
SetI ASST 6 cut(s) 228, 324, 411, 499, 642, 685
SfaNI GCATC 1 cut(s) 676
SfoI GGCGCC 1 cut(s) 462
Sfr303I CCGCGG 2 cut(s) 288, 306
SfuI TTCGAA 1 cut(s) 374
SgrAI CRCCGGYG 1 cut(s) 13
SgrBI CCGCGG 2 cut(s) 288, 306
SinI GGWCC 3 cut(s) 425, 497, 588
Sse9I AATT 3 cut(s) 241, 558, 646
SspDI GGCGCC 1 cut(s) 460
StyD4I CCNGG 4 cut(s) 229, 584, 599, 655
TaaI ACNGT 2 cut(s) 126, 278
TaqI TCGA 3 cut(s) 374, 470, 642
TasI AATT 3 cut(s) 241, 558, 646
TfiI GAWTC 3 cut(s) 134, 337, 704
TseI GCWGC 4 cut(s) 28, 148, 409, 454
TspDTI ATGAA 2 cut(s) 565, 570
Tth111I GACNNNGTC 1 cut(s) 586
VpaK11BI GGWCC 3 cut(s) 425, 497, 588
XapI RAATTY 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.