Rh1BG213900

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0020737
Physical Location & Seq
Forward (+)
33339321 .. 33343847
4527 bp
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UTR
Exon/CDS
Intron
Rh1BG213900.1

Sequence Viewer

Length: 567 bp
ATGGCTGCCTCTCGGGCATCGTCATCAGGTACTTCTCCAAAACTCTCCATAGTAGCGCATGCAGTAAACTTGAGGCCACGAATGAACCCGCAAATGTTGTTGGATAATACCATTGGGAATATCTTTTTGTGGGCTCCGGTGTTATTAGATATCTTGGAAGAGACAACAACAAAGAGTAGTGATCATGAACTGTGTGACTTGGTGAATATGCTTCATGAAACCCTCAACGAATTTAACAATGAGTACTTGGAGACTCTGAAGGATAAAGAAGGATTTGGAGCCATTTGTGACTTACTAGATTTGATGGAGGAAGGGACTTCTATAAAACCACCACCAGAGATTTATGCATTCACGAGCTGGACTAGAATTCTTAACAAACTAGATTTTGGATGGGGAAAGCCATTTCAGATTGGAGTCATCGGAAAAGTTGGACCTGTATTTAGAAATCTAACGATATTCGTACAATGCGATCAAGAAATTGAAGCATGGGTGACTTTGGAGGAAAAACAAATGGCTATGCTAGAAAAAGATTCTCAATTTCTAGCATTTGCATCTCCAAATCCCTAA

Protein Analysis

188

Amino Acids

21.11

Weight (kDa)

4.66

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 180 8.4e-15 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020737)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003588.1_g000004
rosa_samantha Rh1AG242700 Rh1BG213900 Rh1CG226500 Rh1CG226900
rosa_wichuraiana Rw1G021260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 89
AcsI RAATTY 2 cut(s) 230, 366
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 3 cut(s) 31, 245, 462
AgsI TTSAA 1 cut(s) 482
AluBI AGCT 1 cut(s) 357
AluI AGCT 1 cut(s) 357
Alw26I GTCTC 2 cut(s) 155, 245
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 230, 366
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 431
AsuHPI GGTGA 2 cut(s) 214, 502
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 136
BauI CACGAG 1 cut(s) 352
BccI CCATC 2 cut(s) 298, 384
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 155, 245
BfaI CTAG 5 cut(s) 296, 363, 380, 521, 542
BglI GCCNNNNNGGC 1 cut(s) 14
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
BmcAI AGTACT 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 431
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 2 cut(s) 135, 280
BmsI GCATC 2 cut(s) 26, 560
BpuEI CTTGAG 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 136
BseGI GGATG 1 cut(s) 395
BshFI GGCC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 328
BsmAI GTCTC 2 cut(s) 155, 245
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 181, 469
BspACI CCGC 1 cut(s) 89
BspANI GGCC 1 cut(s) 76
BspHI TCATGA 2 cut(s) 184, 214
BspLI GGNNCC 2 cut(s) 135, 280
BssMI GATC 2 cut(s) 181, 469
BssSI CACGAG 1 cut(s) 352
Bst2BI CACGAG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 60
BstF5I GGATG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 184, 472
BstMAI GTCTC 2 cut(s) 155, 245
BstMBI GATC 2 cut(s) 181, 469
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 62
BsuRI GGCC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 395
Cac8I GCNNGC 1 cut(s) 60
CciI TCATGA 2 cut(s) 184, 214
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 431
Csp6I GTAC 3 cut(s) 30, 244, 461
CviAII CATG 4 cut(s) 59, 185, 215, 486
CviJI RGCY 7 cut(s) 5, 76, 134, 281, 357, 400, 515
CviKI_1 RGCY 7 cut(s) 5, 76, 134, 281, 357, 400, 515
CviQI GTAC 3 cut(s) 30, 244, 461
DpnI GATC 2 cut(s) 183, 471
DpnII GATC 2 cut(s) 181, 469
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 366
EcoRV GATATC 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 136
FaeI CATG 4 cut(s) 62, 188, 218, 489
FaiI YATR 9 cut(s) 50, 60, 186, 209, 216, 323, 345, 487, 518
FaqI GGGAC 1 cut(s) 328
FatI CATG 4 cut(s) 58, 184, 214, 485
FauI CCCGC 1 cut(s) 96
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 402
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 5 cut(s) 296, 363, 380, 521, 542
GlaI GCGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 137
HhaI GCGC 1 cut(s) 58
Hin1II CATG 4 cut(s) 62, 188, 218, 489
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 3 cut(s) 253, 414, 530
HpaII CCGG 1 cut(s) 137
HphI GGTGA 2 cut(s) 214, 502
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 258, 408, 422
Hpy188III TCNNGA 4 cut(s) 185, 215, 352, 473
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 3 cut(s) 253, 263, 305
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 62, 347, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 4 cut(s) 62, 188, 218, 489
HspAI GCGC 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 181, 469
LmnI GCTCC 2 cut(s) 139, 278
LpnPI CCDG 5 cut(s) 12, 150, 343, 348, 447
LweI GCATC 2 cut(s) 26, 560
MaeI CTAG 5 cut(s) 296, 363, 380, 521, 542
MaeIII GTNAC 3 cut(s) 194, 287, 490
MalI GATC 2 cut(s) 183, 471
MboI GATC 2 cut(s) 181, 469
MboII GAAGA 1 cut(s) 170
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 4 cut(s) 230, 366, 477, 536
MlyI GAGTC 2 cut(s) 247, 423
MmeI TCCRAC 2 cut(s) 81, 409
MnlI CCTC 5 cut(s) 19, 66, 233, 301, 493
Mph1103I ATGCAT 1 cut(s) 349
MseI TTAA 2 cut(s) 234, 372
MspI CCGG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 347
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 2 cut(s) 181, 469
NlaIII CATG 4 cut(s) 62, 188, 218, 489
NlaIV GGNNCC 2 cut(s) 135, 280
NmuCI GTSAC 3 cut(s) 194, 287, 490
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 1 cut(s) 62
PaeI GCATGC 1 cut(s) 62
PagI TCATGA 2 cut(s) 184, 214
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PctI GAATGC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 247, 422
PpsI GAGTC 2 cut(s) 247, 422
PspN4I GGNNCC 2 cut(s) 135, 280
PspPI GGNCC 1 cut(s) 431
RsaI GTAC 3 cut(s) 31, 245, 462
RsaNI GTAC 3 cut(s) 30, 244, 461
SaqAI TTAA 2 cut(s) 234, 372
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 181, 469
Sau96I GGNCC 1 cut(s) 431
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 2 cut(s) 247, 423
SduI GDGCHC 1 cut(s) 136
SetI ASST 3 cut(s) 31, 359, 436
SfaNI GCATC 2 cut(s) 26, 560
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
SphI GCATGC 1 cut(s) 62
Sse9I AATT 4 cut(s) 230, 366, 477, 536
SsiI CCGC 1 cut(s) 89
SspMI CTAG 5 cut(s) 296, 363, 380, 521, 542
TaaI ACNGT 1 cut(s) 192
TasI AATT 4 cut(s) 230, 366, 477, 536
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 2 cut(s) 234, 372
Tru9I TTAA 2 cut(s) 234, 372
TseFI GTSAC 3 cut(s) 194, 287, 490
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 194, 287, 490
TspDTI ATGAA 4 cut(s) 98, 201, 203, 231
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 2 cut(s) 230, 366
XceI RCATGY 1 cut(s) 62
XspI CTAG 5 cut(s) 296, 363, 380, 521, 542
ZrmI AGTACT 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.