Rh1CG226500

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0020737
Physical Location & Seq
Forward (+)
47954771 .. 47959622
4852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG226500.1

Sequence Viewer

Length: 519 bp
ATGGAGAGTTTGTGGTTCGAGCAGAAAAAGGGTTACGTTACAAGGAGATTTCTGTTTGATGCTAAAGCCATATCCGCACTTCAAGCCAAAGCAAAGAGCGAGCCCGTGCCAAAACCATCACGTATTGAGGCACTTACATGTTTTCTCTGGAAACATCAAATGGCTGCCTCTCGGGCATCGTCATCAGTTACTTCTCCAAAACTCTCCATAGTAGCGCATGCAGTAAATTTGAGGCCACGAATGAACCCGCAAATGTTGTTGGATAATACCATTGGGAATATCATTTTGTGGGCTCCGGTGTTATTAGATATCTTGGAAGAGACAGCAACAAAGAGTGGTGATCATGAATTCTGTGATTTGGTGAACATGCTTCATGAATCCCTCAAGGAATTTAACAATGAGTACTTGGAGACTCTGAAGGGTAAAGAAGGATTTGGAGCCATTTGTGACTTACTAGATTTGATGGAGGAAGGGACTTCTATAAAACCACCGCTCGAGATTTATGCATTCACGAGCTAG

Protein Analysis

172

Amino Acids

19.33

Weight (kDa)

5.83

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 8 - 138 5.8e-14 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020737)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003588.1_g000004
rosa_samantha Rh1AG242700 Rh1BG213900 Rh1CG226500 Rh1CG226900
rosa_wichuraiana Rw1G021260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 493
AciI CCGC 3 cut(s) 75, 248, 491
AcsI RAATTY 3 cut(s) 226, 347, 389
AcuI CTGAAG 1 cut(s) 437
AfaI GTAC 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 137
AgsI TTSAA 1 cut(s) 83
AluBI AGCT 1 cut(s) 516
AluI AGCT 1 cut(s) 516
Alw26I GTCTC 2 cut(s) 314, 404
Ama87I CYCGRG 2 cut(s) 171, 494
AoxI GGCC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 3 cut(s) 226, 347, 389
AspLEI GCGC 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 350, 373
AvaI CYCGRG 2 cut(s) 171, 494
BanII GRGCYC 2 cut(s) 105, 295
BauI CACGAG 1 cut(s) 511
BbvI GCAGC 1 cut(s) 151
BccI CCATC 2 cut(s) 124, 457
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 2 cut(s) 314, 404
BfaI CTAG 2 cut(s) 455, 517
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
BmcAI AGTACT 1 cut(s) 404
BmeT110I CYCGRG 2 cut(s) 171, 494
BmiI GGNNCC 2 cut(s) 294, 439
BmsI GCATC 2 cut(s) 49, 185
BpuEI CTTGAG 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 122
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 1 cut(s) 26
BseXI GCAGC 1 cut(s) 151
BshFI GGCC 1 cut(s) 235
BsiHKCI CYCGRG 2 cut(s) 171, 494
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 487
BsmAI GTCTC 2 cut(s) 314, 404
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 1 cut(s) 235
BsoBI CYCGRG 2 cut(s) 171, 494
Bsp1286I GDGCHC 2 cut(s) 105, 295
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 3 cut(s) 75, 248, 491
BspANI GGCC 1 cut(s) 235
BspHI TCATGA 2 cut(s) 343, 373
BspLI GGNNCC 2 cut(s) 294, 439
BsrBI CCGCTC 1 cut(s) 493
BssMI GATC 1 cut(s) 340
BssSI CACGAG 1 cut(s) 511
Bst2BI CACGAG 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 101, 219
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 2 cut(s) 314, 404
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 3 cut(s) 74, 83, 173
BstNSI RCATGY 3 cut(s) 141, 221, 370
BstV1I GCAGC 1 cut(s) 151
BsuRI GGCC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 101, 219
CciI TCATGA 2 cut(s) 343, 373
CfoI GCGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 403
CviAII CATG 5 cut(s) 138, 218, 344, 367, 374
CviJI RGCY 8 cut(s) 68, 86, 103, 164, 235, 293, 440, 516
CviKI_1 RGCY 8 cut(s) 68, 86, 103, 164, 235, 293, 440, 516
CviQI GTAC 1 cut(s) 403
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Eco24I GRGCYC 2 cut(s) 105, 295
Eco32I GATATC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 437
Eco88I CYCGRG 2 cut(s) 171, 494
EcoRI GAATTC 1 cut(s) 347
EcoRV GATATC 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 508
EcoT38I GRGCYC 2 cut(s) 105, 295
FaeI CATG 5 cut(s) 141, 221, 347, 370, 377
FaiI YATR 9 cut(s) 71, 139, 209, 219, 345, 368, 375, 482, 504
FaqI GGGAC 1 cut(s) 487
FatI CATG 5 cut(s) 137, 217, 343, 366, 373
FauI CCCGC 1 cut(s) 255
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 1 cut(s) 165
FriOI GRGCYC 2 cut(s) 105, 295
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 455, 517
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 235
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 217
Hin1II CATG 5 cut(s) 141, 221, 347, 370, 377
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 2 cut(s) 377, 412
HpaII CCGG 1 cut(s) 296
HphI GGTGA 2 cut(s) 350, 373
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 5 cut(s) 148, 344, 374, 496, 511
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 3 cut(s) 412, 422, 464
HpyCH4IV ACGT 2 cut(s) 36, 121
HpyCH4V TGCA 2 cut(s) 221, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 83, 173
HpySE526I ACGT 2 cut(s) 36, 121
Hsp92II CATG 5 cut(s) 141, 221, 347, 370, 377
HspAI GCGC 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 2 cut(s) 298, 437
LpnPI CCDG 2 cut(s) 133, 309
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 2 cut(s) 49, 185
MaeI CTAG 2 cut(s) 455, 517
MaeII ACGT 2 cut(s) 36, 121
MaeIII GTNAC 4 cut(s) 32, 37, 187, 446
MalI GATC 1 cut(s) 342
MbiI CCGCTC 1 cut(s) 493
MboI GATC 1 cut(s) 340
MboII GAAGA 1 cut(s) 329
MhlI GDGCHC 2 cut(s) 105, 295
MluCI AATT 3 cut(s) 226, 347, 389
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 5 cut(s) 121, 178, 225, 392, 460
Mph1103I ATGCAT 1 cut(s) 508
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 136
MspI CCGG 1 cut(s) 296
Mva1269I GAATGC 1 cut(s) 506
MwoI GCNNNNNNNGC 3 cut(s) 74, 83, 173
NdeII GATC 1 cut(s) 340
NlaIII CATG 5 cut(s) 141, 221, 347, 370, 377
NlaIV GGNNCC 2 cut(s) 294, 439
NmuCI GTSAC 1 cut(s) 446
NsiI ATGCAT 1 cut(s) 508
NspI RCATGY 3 cut(s) 141, 221, 370
PaeI GCATGC 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 494
PagI TCATGA 2 cut(s) 343, 373
PciI ACATGT 1 cut(s) 137
PctI GAATGC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 377
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Ppu21I YACGTR 1 cut(s) 122
PscI ACATGT 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 294, 439
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 136
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 340
ScaI AGTACT 1 cut(s) 404
SchI GAGTC 1 cut(s) 406
SduI GDGCHC 2 cut(s) 105, 295
SetI ASST 3 cut(s) 39, 124, 518
SfaNI GCATC 2 cut(s) 49, 185
Sfr274I CTCGAG 1 cut(s) 494
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 136
SmlI CTYRAG 2 cut(s) 383, 494
SmoI CTYRAG 2 cut(s) 383, 494
SphI GCATGC 1 cut(s) 221
Sse9I AATT 3 cut(s) 226, 347, 389
SsiI CCGC 3 cut(s) 75, 248, 491
SspMI CTAG 2 cut(s) 455, 517
TaiI ACGT 2 cut(s) 39, 124
TaqI TCGA 2 cut(s) 18, 495
TasI AATT 3 cut(s) 226, 347, 389
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 4 cut(s) 257, 360, 362, 390
XapI RAATTY 3 cut(s) 226, 347, 389
XceI RCATGY 3 cut(s) 141, 221, 370
XhoI CTCGAG 1 cut(s) 494
XspI CTAG 2 cut(s) 455, 517
ZrmI AGTACT 1 cut(s) 404
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.