Rh1CG031100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0018537
Physical Location & Seq
Forward (+)
6185619 .. 6189615
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG031100.1

Sequence Viewer

Length: 1041 bp
ATGGCTGGCGTAGTGGGTGGAGCTTTCCTCTCTGCTTCCCTTCAGGTCTTGTTCGATAGATTGGCTTCTCGTCAAGTCGTTGACTTCATCCGCGGCAACAAGCTCAACGCTGGACTTCTCAGAAAGCTAGAAATCACTCTGCTTTCTGTCAATTCCGTGCTCAGTGATGCCGAGGACAAGCAGATAACCAATCATGCTGTGAAAGAATGGCTTGATGAGCTTAGAGCTGCTGTCTATGATGCCGAGGACCTCATGGCTGACTTCAGCTCTGAAGCTTTGGAGAAAGAGCTTCAAGAGGAATCTGAATCCGGAAGCAGCGGCGGAATTCAGGTAAAAGATGTCAAATTGTATTTAATGAATGCATTGGATGAATGGTTGGAGCCCAAGATAGAGGAAGTTGTTGAAAGACTAGAATTTATTGCTAAGAAGAAGTATGTTCTTGGATTGAAAGAGGGTATTAAAGATGTAGCAGTAAGAAGATTGCCTGCAACTTCTTTGGTGGAGGAGTCTAGTGTGTATGGCAGGGATGAGGACAGAGAGCTGGTGGCCTCCCTTCCAAGGGCTCCATCTATCCGTGATTTGAAGTTTGGATATTGTGATGGGTTGCAGTTAAAAGAATTGCCTTCTGAACTGCACTGGCTCAGAATTGAAGGATGCCATGTCCTGGAGTCTATACCTGGTCATGTGAAGGAAACAAATCCTTGTCTTCAGGAGTTATTTATCTCTGACTGTGCTTCTCTGGTGTCCTTTCCTAAGGATGGATTACCTGCTACACTGAAAGCCATTGAAATTGAGAACTGTGGGAGGTTAGAGATACCTGTTGACCACTACTATGCTTCCCTTGAAAGCTTGGCCATATGGAGTAGTTGCGATTCTTTAGAGTCCTTTCCATTGGAACTGTTCCCAAACTTGAACAATCTCAAAATTCGGAGCTGCAAAGGTCTAAAATCACTTTCTTTTTCAGTTTCTGTGTACTTAACCATGACAATGCTGGAAGCTATTCTGGTTTCAAGCTCAAAGAGATATGCTATAGCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

346

Amino Acids

38.46

Weight (kDa)

4.91

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 99 2.9e-18 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018537)

Species Orthologous Gene IDs
pyrus_communis pycom10g28390
rosa_multiflora Rmu_sc0000060.1_g000053 Rmu_sc0001706.1_g000037
rosa_roxburghii Rroxscaffold_1G00075220
rosa_samantha Rh1AG032700 Rh1CG031100 Rh1DG038400 Rh1DG095500 Rh6BG326800 Rh6CG415600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 775
AccB7I CCANNNNNTGG 1 cut(s) 664
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 308
AciI CCGC 4 cut(s) 91, 93, 318, 321
AcoI YGGCCR 1 cut(s) 852
AcsI RAATTY 3 cut(s) 324, 413, 924
AcuI CTGAAG 4 cut(s) 26, 247, 291, 692
AfaI GTAC 1 cut(s) 974
AfiI CCNNNNNNNGG 4 cut(s) 558, 559, 664, 758
AgsI TTSAA 9 cut(s) 293, 404, 448, 583, 650, 788, 845, 913, 1011
AjnI CCWGG 2 cut(s) 663, 676
Alw21I GWGCWC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 968
Aor13HI TCCGGA 1 cut(s) 308
AoxI GGCC 2 cut(s) 546, 852
ApeKI GCWGC 3 cut(s) 227, 315, 933
ApoI RAATTY 3 cut(s) 324, 413, 924
Asp700I GAANNNNTTC 1 cut(s) 999
AspS9I GGNCC 1 cut(s) 247
AvaII GGWCC 1 cut(s) 247
AxyI CCTNAGG 1 cut(s) 753
BalI TGGCCA 1 cut(s) 854
BanII GRGCYC 2 cut(s) 384, 565
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 214, 327, 920
BccI CCATC 3 cut(s) 574, 593, 752
BciT130I CCWGG 2 cut(s) 665, 678
BfaI CTAG 3 cut(s) 128, 410, 510
BfmI CTRYAG 1 cut(s) 1029
BfuAI ACCTGC 1 cut(s) 775
BisI GCNGC 5 cut(s) 94, 228, 316, 319, 934
BlsI GCNGC 5 cut(s) 95, 229, 317, 320, 935
Bme1390I CCNGG 2 cut(s) 665, 678
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 381, 564
BmrFI CCNGG 2 cut(s) 665, 678
BmsI GCATC 3 cut(s) 157, 229, 644
BpiI GAAGAC 1 cut(s) 698
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpmI CTGGAG 1 cut(s) 686
BsaJI CCNNGG 4 cut(s) 91, 171, 243, 557
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 4 cut(s) 558, 559, 664, 758
Bse1I ACTGG 1 cut(s) 641
Bse21I CCTNAGG 1 cut(s) 753
BseAI TCCGGA 1 cut(s) 308
BseBI CCWGG 2 cut(s) 665, 678
BseDI CCNNGG 4 cut(s) 91, 171, 243, 557
BseGI GGATG 5 cut(s) 87, 373, 532, 659, 763
BseLI CCNNNNNNNGG 4 cut(s) 558, 559, 664, 758
BseMII CTCAG 3 cut(s) 133, 175, 655
BseNI ACTGG 1 cut(s) 641
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 3 cut(s) 214, 327, 920
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 548, 854
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 4 cut(s) 558, 559, 664, 758
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 2 cut(s) 548, 854
Bsp1286I GDGCHC 3 cut(s) 162, 384, 565
Bsp13I TCCGGA 1 cut(s) 308
BspACI CCGC 4 cut(s) 91, 93, 318, 321
BspANI GGCC 2 cut(s) 548, 854
BspCNI CTCAG 3 cut(s) 132, 174, 654
BspEI TCCGGA 1 cut(s) 308
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 381, 564
BspMI ACCTGC 1 cut(s) 775
BsrI ACTGG 1 cut(s) 641
BssECI CCNNGG 4 cut(s) 91, 171, 243, 557
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 2 cut(s) 665, 678
Bst4CI ACNGT 3 cut(s) 731, 800, 900
BstC8I GCNNGC 2 cut(s) 7, 486
BstDEI CTNAG 6 cut(s) 119, 161, 221, 423, 641, 753
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 5 cut(s) 87, 373, 532, 659, 763
BstFNI CGCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 2 cut(s) 665, 678
BstSCI CCNGG 2 cut(s) 663, 676
BstSFI CTRYAG 1 cut(s) 1029
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 214, 327, 920
BstV2I GAAGAC 1 cut(s) 698
Bsu36I CCTNAGG 1 cut(s) 753
BsuRI GGCC 2 cut(s) 548, 854
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 5 cut(s) 87, 373, 532, 659, 763
BtsIMutI CAGTG 3 cut(s) 169, 634, 773
BveI ACCTGC 1 cut(s) 775
Cac8I GCNNGC 2 cut(s) 7, 486
CaiI CAGNNNCTG 1 cut(s) 968
Cfr13I GGNCC 1 cut(s) 247
Cfr42I CCGCGG 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 676
Csp6I GTAC 1 cut(s) 973
CviAII CATG 5 cut(s) 194, 253, 659, 683, 982
CviQI GTAC 1 cut(s) 973
DdeI CTNAG 6 cut(s) 119, 161, 221, 423, 641, 753
EaeI YGGCCR 1 cut(s) 852
EciI GGCGGA 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 557
Eco24I GRGCYC 2 cut(s) 384, 565
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 4 cut(s) 26, 247, 291, 692
Eco81I CCTNAGG 1 cut(s) 753
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 324
EcoRII CCWGG 2 cut(s) 663, 676
EcoT14I CCWWGG 1 cut(s) 557
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 2 cut(s) 384, 565
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 5 cut(s) 197, 256, 662, 686, 985
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FatI CATG 5 cut(s) 193, 252, 658, 682, 981
FauNDI CATATG 1 cut(s) 857
Fnu4HI GCNGC 5 cut(s) 94, 228, 316, 319, 934
FokI GGATG 5 cut(s) 74, 380, 539, 666, 770
FriOI GRGCYC 2 cut(s) 384, 565
Fsp4HI GCNGC 5 cut(s) 94, 228, 316, 319, 934
FspBI CTAG 3 cut(s) 128, 410, 510
GluI GCNGC 5 cut(s) 94, 228, 316, 319, 934
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 2 cut(s) 548, 854
HapII CCGG 1 cut(s) 309
Hin1II CATG 5 cut(s) 197, 256, 662, 686, 985
HincII GTYRAC 2 cut(s) 82, 823
HindII GTYRAC 2 cut(s) 82, 823
HindIII AAGCTT 2 cut(s) 273, 847
HinfI GANTC 6 cut(s) 299, 305, 506, 668, 872, 881
HpaII CCGG 1 cut(s) 309
Hpy166II GTNNAC 3 cut(s) 82, 823, 973
Hpy188I TCNGA 7 cut(s) 122, 271, 304, 628, 644, 727, 930
Hpy188III TCNNGA 3 cut(s) 293, 309, 710
Hpy8I GTNNAC 3 cut(s) 82, 823, 973
HpyAV CCTTC 5 cut(s) 50, 563, 633, 644, 682
HpyCH4III ACNGT 3 cut(s) 731, 800, 900
HpyCH4V TGCA 5 cut(s) 362, 488, 607, 634, 936
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 6 cut(s) 119, 161, 221, 423, 641, 753
Hsp92II CATG 5 cut(s) 197, 256, 662, 686, 985
Kpn2I TCCGGA 1 cut(s) 308
KspI CCGCGG 1 cut(s) 94
LmnI GCTCC 4 cut(s) 20, 379, 568, 930
Lsp1109I GCAGC 3 cut(s) 214, 327, 920
LweI GCATC 3 cut(s) 157, 229, 644
MabI ACCWGGT 1 cut(s) 676
MaeI CTAG 3 cut(s) 128, 410, 510
MboII GAAGA 3 cut(s) 439, 489, 698
MhlI GDGCHC 3 cut(s) 162, 384, 565
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 8 cut(s) 151, 324, 344, 413, 617, 645, 789, 924
MluNI TGGCCA 1 cut(s) 854
MlyI GAGTC 3 cut(s) 515, 677, 890
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 1 cut(s) 854
Mph1103I ATGCAT 1 cut(s) 364
MroI TCCGGA 1 cut(s) 308
MroXI GAANNNNTTC 1 cut(s) 999
MscI TGGCCA 1 cut(s) 854
MseI TTAA 4 cut(s) 353, 459, 611, 977
MslI CAYNNNNRTG 2 cut(s) 831, 986
Msp20I TGGCCA 1 cut(s) 854
MspA1I CMGCKG 2 cut(s) 93, 318
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 665, 678
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 2 cut(s) 665, 678
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 217
NdeI CATATG 1 cut(s) 857
NlaIII CATG 5 cut(s) 197, 256, 662, 686, 985
NlaIV GGNNCC 2 cut(s) 381, 564
NmeAIII GCCGAG 2 cut(s) 196, 268
NsiI ATGCAT 1 cut(s) 364
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 3 cut(s) 299, 305, 872
PflMI CCANNNNNTGG 1 cut(s) 664
PfoI TCCNGGA 1 cut(s) 663
PkrI GCNGC 5 cut(s) 95, 229, 317, 320, 935
PleI GAGTC 3 cut(s) 514, 676, 889
PpsI GAGTC 3 cut(s) 514, 676, 889
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 663, 676
PspGI CCWGG 2 cut(s) 663, 676
PspN4I GGNNCC 2 cut(s) 381, 564
PspPI GGNCC 1 cut(s) 247
PspPPI RGGWCCY 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 968
RsaI GTAC 1 cut(s) 974
RsaNI GTAC 1 cut(s) 973
RseI CAYNNNNRTG 2 cut(s) 831, 986
SacII CCGCGG 1 cut(s) 94
SaqAI TTAA 4 cut(s) 353, 459, 611, 977
SatI GCNGC 5 cut(s) 94, 228, 316, 319, 934
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 3 cut(s) 515, 677, 890
ScrFI CCNGG 2 cut(s) 665, 678
SduI GDGCHC 3 cut(s) 162, 384, 565
SexAI ACCWGGT 1 cut(s) 676
SfaNI GCATC 3 cut(s) 157, 229, 644
SfcI CTRYAG 1 cut(s) 1029
Sfr303I CCGCGG 1 cut(s) 94
SgrBI CCGCGG 1 cut(s) 94
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 831, 986
Sse9I AATT 8 cut(s) 151, 324, 344, 413, 617, 645, 789, 924
SsiI CCGC 4 cut(s) 91, 93, 318, 321
SspMI CTAG 3 cut(s) 128, 410, 510
StyD4I CCNGG 2 cut(s) 663, 676
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 3 cut(s) 731, 800, 900
TaqI TCGA 1 cut(s) 54
TasI AATT 8 cut(s) 151, 324, 344, 413, 617, 645, 789, 924
TatI WGTACW 1 cut(s) 972
TauI GCSGC 2 cut(s) 96, 321
TfiI GAWTC 3 cut(s) 299, 305, 872
Tru1I TTAA 4 cut(s) 353, 459, 611, 977
Tru9I TTAA 4 cut(s) 353, 459, 611, 977
TscAI CASTG 3 cut(s) 169, 641, 780
TseI GCWGC 3 cut(s) 227, 315, 933
TspDTI ATGAA 3 cut(s) 76, 371, 384
TspGWI ACGGA 2 cut(s) 145, 563
TspRI CASTG 3 cut(s) 169, 641, 780
Van91I CCANNNNNTGG 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 247
XapI RAATTY 3 cut(s) 324, 413, 924
XcmI CCANNNNNNNNNTGG 1 cut(s) 988
XmnI GAANNNNTTC 1 cut(s) 999
XspI CTAG 3 cut(s) 128, 410, 510
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.