Rh1CG309100

Sulfate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007250
Physical Location & Seq
Reverse (-)
58329529 .. 58337806
8278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG309100.1

Sequence Viewer

Length: 1668 bp
ATGGAGATAACCTACGCAACCCCAAGCAACTCCGATTTAGCGGGCGGATCCTCCTCCATGCCGACGACGACCCGACCCGTCCGGATTATCCCCTTGCAGCACCCGGAAACGTCGTCGGTGTCGTCGTCGTCCTCCTCAAATGCGTCGCCGCTGTGGGCCGCGCTGTCGAGGTGGAAATCGAAGGTCGTCAGCATGACGTGGATTGAGTGGCTGGAGCTGTTTCTGCCTTGCTTCCGGTGGATTCGGACCTACAAATGGCGCGAGTATCTCCAGGTCGATCTCATGGCCGGAATGACCGTCGGCGTCATGCTCGTTCCTCAGGCAATGTCTTATGCAAAATTGGCTGGGCTTGAACCCATATATGGACTTTACTCTGGATTTGTACCTTTATTTGTGTATGCTATATTTGGCTCGTCTCGTCAGCTAGCTGTTGGTCCAGTAGCGTTGGTTTCACTCCTAGTCTCCAATGTTTTAAGTGGCATCGTTGATTCTTCTGATGTGTTATACACTGAGCTGGCAATATTATTGGCACTCATGGTTGGGATCATGGAATGCATTTTGGGGCTTTTAAGGCTTGGATGGATTATTCGCTTCATCAGCCACTCAGTCATTTCTGGTTTTACGACTGCTTCAGCCATTGTGATTGCTTTATCTCAAGCAAAATACTTCTTGGGATATGATGTAGAACGAAGTAGCGAGATTGTGCCACTGATTGAGAGCATTATATCTGGAGCTGATGCGTTTTCATGGCCCCCTTTCGTAATGGGATCCGTCATTCTTGCGATACTATTGACCATGAAACACTTGGGAAAGACAAAGAAACACTTGCGTTTCCTGAGAGCAGCAGGTCCCCTCACTGCAGTCCTTTTGGGCACTATTTTTGTAAAAATATTTAATCCATCTTCCATATCCTTGGTAGGAGACATACCGCAGGGCCTTCCATCATTTTCCATCCCTAAAGCTTTTGGATATGCTACATCTTTGATTCCGACTGCGCTTCTCATTACTGGTGTAGCTATTTTGGAATCTGTGGGCATTGCAAAAGCATTAGCAGCAAAGAATGGCTATGAGTTGGATTCAAATCAAGAGTTGTTCGGTCTTGGGGTGGCAAATATTTTTGGTTCATTTTTTTCTGCATACCCTACAACAGGTTCCTTTTCTAGGTCAGCTGTGAATAATGAAAGTGGAGCAAAAACTGGCTTATCTGGAATTGTTATGGGATTTATAATGGGGTGTGCCCTACTATTTATGACTACACTGTTTGAATCCATACCCCAGTGTGCTCTAGCTGCCATTGTCATTTCTGCTGTAATAGGCCTGGTGGATTATGAGGAGGCTATATTTTTATGGCGAGTGGATAAGAAAGATTTTCTTCTTTGGACCATCACAAGCAGTACAACTTTGTTCCTTGGCATTGAGATTGGCGTCCTTGTCGGGGTTGGTGTTTCACTTGCATTTGTTATATATGAATCAGCAAACCCCCATATTGCTGTCTTGGGGCGTCTCCCAGGTACCACTGTTTACAGAAATACACAACAATATCCAGAAGCATATACATACAATGGAATTGTGATTGTTCGGATTGATGCACCTATTTACTTTGCTAACATTAGTTACATAAAAGACAGGTATAATTTCATGCGATCTTATTTTGATAGCAAACATTAA

Protein Analysis

555

Amino Acids

60.25

Weight (kDa)

8.17

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfate_transp PF00916 90 - 463 3e-128 Sulfate permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1226
Acc36I ACCTGC 1 cut(s) 836
Acc65I GGTACC 1 cut(s) 1511
AccB1I GGYRCC 1 cut(s) 1511
AccII CGCG 2 cut(s) 161, 261
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 5 cut(s) 41, 45, 149, 159, 929
AclWI GGATC 5 cut(s) 42, 55, 551, 762, 775
AcoI YGGCCR 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 615
AcyI GRCGYC 3 cut(s) 303, 1425, 1501
AfaI GTAC 3 cut(s) 384, 1396, 1513
AfiI CCNNNNNNNGG 5 cut(s) 255, 362, 1148, 1161, 1435
AgsI TTSAA 3 cut(s) 353, 1080, 1265
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 3 cut(s) 270, 1317, 1507
AluBI AGCT 9 cut(s) 217, 424, 428, 514, 734, 962, 1016, 1169, 1289
AluI AGCT 9 cut(s) 217, 424, 428, 514, 734, 962, 1016, 1169, 1289
Alw21I GWGCWC 1 cut(s) 1285
Alw26I GTCTC 4 cut(s) 420, 466, 915, 1508
AlwI GGATC 5 cut(s) 42, 55, 551, 762, 775
Aor13HI TCCGGA 1 cut(s) 81
AoxI GGCC 5 cut(s) 156, 285, 749, 934, 1315
ApeKI GCWGC 4 cut(s) 97, 842, 1052, 1289
Asp718I GGTACC 1 cut(s) 1511
AspLEI GCGC 3 cut(s) 163, 261, 997
AspS9I GGNCC 7 cut(s) 156, 246, 434, 750, 848, 934, 1380
AsuC2I CCSGG 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 424
AvaII GGWCC 4 cut(s) 246, 434, 848, 1380
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 2 cut(s) 875, 1240
BaeI ACNNNNGTAYC 2 cut(s) 1503, 1536
BamHI GGATCC 2 cut(s) 47, 767
BanI GGYRCC 1 cut(s) 1511
Bbv12I GWGCWC 1 cut(s) 1285
BbvI GCAGC 4 cut(s) 109, 854, 1064, 1276
BccI CCATC 5 cut(s) 573, 907, 949, 959, 1391
BciT130I CCWGG 3 cut(s) 272, 1319, 1509
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 4 cut(s) 420, 466, 915, 1508
BfaI CTAG 4 cut(s) 425, 458, 1161, 1286
BfmI CTRYAG 1 cut(s) 858
BfuAI ACCTGC 1 cut(s) 836
BisI GCNGC 6 cut(s) 98, 149, 159, 843, 1053, 1290
BlsI GCNGC 6 cut(s) 99, 150, 160, 844, 1054, 1291
Bme1390I CCNGG 4 cut(s) 104, 272, 1319, 1509
Bme18I GGWCC 4 cut(s) 246, 434, 848, 1380
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 7 cut(s) 156, 246, 434, 750, 848, 934, 1380
BmiI GGNNCC 6 cut(s) 49, 752, 769, 850, 1153, 1513
BmrFI CCNGG 4 cut(s) 104, 272, 1319, 1509
BmrI ACTGGG 1 cut(s) 1270
BmsI GCATC 3 cut(s) 489, 727, 1576
BmtI GCTAGC 1 cut(s) 428
BmuI ACTGGG 1 cut(s) 1270
BpmI CTGGAG 3 cut(s) 233, 254, 750
BpuEI CTTGAG 1 cut(s) 639
BpuMI CCSGG 1 cut(s) 104
BsaBI GATNNNNATC 1 cut(s) 1080
BsaHI GRCGYC 3 cut(s) 303, 1425, 1501
BsaJI CCNNGG 3 cut(s) 912, 1408, 1507
BsaWI WCCGGW 2 cut(s) 81, 234
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 362, 1148, 1161, 1435
Bse1I ACTGG 4 cut(s) 437, 1012, 1201, 1276
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 2 cut(s) 330, 1035
Bse8I GATNNNNATC 1 cut(s) 1080
BseAI TCCGGA 1 cut(s) 81
BseBI CCWGG 3 cut(s) 272, 1319, 1509
BseDI CCNNGG 3 cut(s) 912, 1408, 1507
BseGI GGATG 2 cut(s) 584, 951
BseJI GATNNNNATC 1 cut(s) 1080
BseLI CCNNNNNNNGG 5 cut(s) 255, 362, 1148, 1161, 1435
BseMI GCAATG 2 cut(s) 330, 1035
BseMII CTCAG 4 cut(s) 332, 501, 618, 827
BseNI ACTGG 4 cut(s) 437, 1012, 1201, 1276
BseRI GAGGAG 3 cut(s) 43, 124, 1346
BseSI GKGCMC 2 cut(s) 875, 1240
BseXI GCAGC 4 cut(s) 109, 854, 1064, 1276
BseYI CCCAGC 1 cut(s) 344
Bsh1236I CGCG 2 cut(s) 161, 261
BshFI GGCC 5 cut(s) 158, 287, 751, 936, 1317
BshNI GGYRCC 1 cut(s) 1511
BsiHKAI GWGCWC 1 cut(s) 1285
BsiSI CCGG 4 cut(s) 82, 104, 235, 288
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 5 cut(s) 255, 362, 1148, 1161, 1435
BsmAI GTCTC 4 cut(s) 420, 466, 915, 1508
BsmBI CGTCTC 2 cut(s) 420, 1508
BsmFI GGGAC 1 cut(s) 834
BsmI GAATGC 1 cut(s) 557
BsnI GGCC 5 cut(s) 158, 287, 751, 936, 1317
Bsp1286I GDGCHC 3 cut(s) 875, 1240, 1285
Bsp13I TCCGGA 1 cut(s) 81
Bsp143I GATC 5 cut(s) 47, 277, 543, 767, 1643
BspACI CCGC 5 cut(s) 41, 45, 149, 159, 929
BspANI GGCC 5 cut(s) 158, 287, 751, 936, 1317
BspCNI CTCAG 4 cut(s) 331, 502, 617, 828
BspEI TCCGGA 1 cut(s) 81
BspFNI CGCG 2 cut(s) 161, 261
BspLI GGNNCC 6 cut(s) 49, 752, 769, 850, 1153, 1513
BspMAI CTGCAG 1 cut(s) 862
BspMI ACCTGC 1 cut(s) 836
BspOI GCTAGC 1 cut(s) 428
BspPI GGATC 5 cut(s) 42, 55, 551, 762, 775
BspT107I GGYRCC 1 cut(s) 1511
BsrDI GCAATG 2 cut(s) 330, 1035
BsrI ACTGG 4 cut(s) 437, 1012, 1201, 1276
BssECI CCNNGG 3 cut(s) 912, 1408, 1507
BssMI GATC 5 cut(s) 47, 277, 543, 767, 1643
BssNI GRCGYC 3 cut(s) 303, 1425, 1501
BssT1I CCWWGG 2 cut(s) 912, 1408
Bst2UI CCWGG 3 cut(s) 272, 1319, 1509
Bst4CI ACNGT 3 cut(s) 298, 1260, 1519
BstACI GRCGYC 3 cut(s) 303, 1425, 1501
BstC8I GCNNGC 3 cut(s) 43, 426, 516
BstDEI CTNAG 4 cut(s) 318, 510, 604, 836
BstENI CCTNNNNNAGG 2 cut(s) 1146, 1159
BstF5I GGATG 2 cut(s) 584, 951
BstFNI CGCG 2 cut(s) 161, 261
BstHHI GCGC 3 cut(s) 163, 261, 997
BstKTI GATC 5 cut(s) 50, 280, 546, 770, 1646
BstMAI GTCTC 4 cut(s) 420, 466, 915, 1508
BstMBI GATC 5 cut(s) 47, 277, 543, 767, 1643
BstMWI GCNNNNNNNGC 6 cut(s) 223, 341, 571, 597, 1052, 1289
BstNI CCWGG 3 cut(s) 272, 1319, 1509
BstSCI CCNGG 4 cut(s) 102, 270, 1317, 1507
BstSFI CTRYAG 1 cut(s) 858
BstSLI GKGCMC 2 cut(s) 875, 1240
BstUI CGCG 2 cut(s) 161, 261
BstV1I GCAGC 4 cut(s) 109, 854, 1064, 1276
BstX2I RGATCY 2 cut(s) 47, 767
BstXI CCANNNNNNTGG 1 cut(s) 913
BstYI RGATCY 2 cut(s) 47, 767
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 5 cut(s) 158, 287, 751, 936, 1317
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 2 cut(s) 584, 951
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 6 cut(s) 507, 707, 855, 1256, 1283, 1515
BveI ACCTGC 1 cut(s) 836
Cac8I GCNNGC 3 cut(s) 43, 426, 516
CfoI GCGC 3 cut(s) 163, 261, 997
Cfr13I GGNCC 7 cut(s) 156, 246, 434, 750, 848, 934, 1380
CseI GACGC 4 cut(s) 132, 292, 1414, 1490
Csp6I GTAC 3 cut(s) 383, 1395, 1512
CviAII CATG 9 cut(s) 58, 193, 283, 307, 535, 547, 747, 796, 1639
CviQI GTAC 3 cut(s) 383, 1395, 1512
DdeI CTNAG 4 cut(s) 318, 510, 604, 836
DpnI GATC 5 cut(s) 49, 279, 545, 769, 1645
DpnII GATC 5 cut(s) 47, 277, 543, 767, 1643
EaeI YGGCCR 1 cut(s) 285
EciI GGCGGA 1 cut(s) 60
Eco130I CCWWGG 2 cut(s) 912, 1408
Eco147I AGGCCT 1 cut(s) 1317
Eco47I GGWCC 4 cut(s) 246, 434, 848, 1380
Eco57I CTGAAG 1 cut(s) 615
Eco81I CCTNAGG 1 cut(s) 318
EcoNI CCTNNNNNAGG 2 cut(s) 1146, 1159
EcoO109I RGGNCCY 2 cut(s) 848, 934
EcoRII CCWGG 3 cut(s) 270, 1317, 1507
EcoT14I CCWWGG 2 cut(s) 912, 1408
EcoT22I ATGCAT 1 cut(s) 557
ErhI CCWWGG 2 cut(s) 912, 1408
Esp3I CGTCTC 2 cut(s) 420, 1508
FaeI CATG 9 cut(s) 61, 196, 286, 310, 538, 550, 750, 799, 1642
FalI AAGNNNNNCTT 4 cut(s) 809, 841, 1356, 1388
FaqI GGGAC 1 cut(s) 834
FatI CATG 9 cut(s) 57, 192, 282, 306, 534, 546, 746, 795, 1638
FauI CCCGC 1 cut(s) 34
Fnu4HI GCNGC 6 cut(s) 98, 149, 159, 843, 1053, 1290
FokI GGATG 2 cut(s) 591, 938
Fsp4HI GCNGC 6 cut(s) 98, 149, 159, 843, 1053, 1290
FspBI CTAG 4 cut(s) 425, 458, 1161, 1286
GlaI GCGC 3 cut(s) 162, 260, 996
GluI GCNGC 6 cut(s) 98, 149, 159, 843, 1053, 1290
GsaI CCCAGC 1 cut(s) 348
GsuI CTGGAG 3 cut(s) 233, 254, 750
HaeIII GGCC 5 cut(s) 158, 287, 751, 936, 1317
HapII CCGG 4 cut(s) 82, 104, 235, 288
HgaI GACGC 4 cut(s) 132, 292, 1414, 1490
HhaI GCGC 3 cut(s) 163, 261, 997
Hin1I GRCGYC 3 cut(s) 303, 1425, 1501
Hin1II CATG 9 cut(s) 61, 196, 286, 310, 538, 550, 750, 799, 1642
Hin6I GCGC 3 cut(s) 161, 259, 995
HinP1I GCGC 3 cut(s) 161, 259, 995
HindIII AAGCTT 1 cut(s) 960
HinfI GANTC 7 cut(s) 241, 488, 985, 1025, 1076, 1265, 1469
HpaII CCGG 4 cut(s) 82, 104, 235, 288
Hpy166II GTNNAC 1 cut(s) 1522
Hpy188I TCNGA 5 cut(s) 34, 246, 496, 990, 1581
Hpy188III TCNNGA 7 cut(s) 82, 375, 729, 835, 1085, 1206, 1544
Hpy8I GTNNAC 1 cut(s) 1522
Hpy99I CGWCG 8 cut(s) 67, 70, 115, 118, 127, 130, 148, 302
HpyAV CCTTC 2 cut(s) 175, 947
HpyCH4III ACNGT 3 cut(s) 298, 1260, 1519
HpyCH4IV ACGT 2 cut(s) 110, 197
HpyCH4V TGCA 8 cut(s) 97, 335, 555, 860, 1040, 1136, 1454, 1589
HpyF10VI GCNNNNNNNGC 6 cut(s) 223, 341, 571, 597, 1052, 1289
HpyF3I CTNAG 4 cut(s) 318, 510, 604, 836
HpySE526I ACGT 2 cut(s) 110, 197
Hsp92I GRCGYC 3 cut(s) 303, 1425, 1501
Hsp92II CATG 9 cut(s) 61, 196, 286, 310, 538, 550, 750, 799, 1642
HspAI GCGC 3 cut(s) 161, 259, 995
Kpn2I TCCGGA 1 cut(s) 81
KpnI GGTACC 1 cut(s) 1515
Kzo9I GATC 5 cut(s) 47, 277, 543, 767, 1643
LmnI GCTCC 3 cut(s) 214, 731, 1187
Lsp1109I GCAGC 4 cut(s) 109, 854, 1064, 1276
LweI GCATC 3 cut(s) 489, 727, 1576
MaeI CTAG 4 cut(s) 425, 458, 1161, 1286
MaeII ACGT 2 cut(s) 110, 197
MaeIII GTNAC 1 cut(s) 1613
MalI GATC 5 cut(s) 49, 279, 545, 769, 1645
MboI GATC 5 cut(s) 47, 277, 543, 767, 1643
MboII GAAGA 3 cut(s) 483, 894, 1364
MflI RGATCY 2 cut(s) 47, 767
MhlI GDGCHC 3 cut(s) 875, 1240, 1285
MluCI AATT 4 cut(s) 338, 1209, 1566, 1633
MmeI TCCRAC 2 cut(s) 1013, 1053
MnlI CCTC 9 cut(s) 61, 64, 142, 145, 162, 327, 863, 1324, 1327
Mph1103I ATGCAT 1 cut(s) 557
MroI TCCGGA 1 cut(s) 81
MseI TTAA 4 cut(s) 473, 569, 894, 1666
MspA1I CMGCKG 2 cut(s) 151, 1169
MspI CCGG 4 cut(s) 82, 104, 235, 288
MspR9I CCNGG 4 cut(s) 104, 272, 1319, 1509
Mva1269I GAATGC 1 cut(s) 557
MvaI CCWGG 3 cut(s) 272, 1319, 1509
MvnI CGCG 2 cut(s) 161, 261
MwoI GCNNNNNNNGC 6 cut(s) 223, 341, 571, 597, 1052, 1289
NciI CCSGG 1 cut(s) 104
NdeII GATC 5 cut(s) 47, 277, 543, 767, 1643
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 9 cut(s) 61, 196, 286, 310, 538, 550, 750, 799, 1642
NlaIV GGNNCC 6 cut(s) 49, 752, 769, 850, 1153, 1513
NsiI ATGCAT 1 cut(s) 557
PceI AGGCCT 1 cut(s) 1317
PcsI WCGNNNNNNNCGW 2 cut(s) 119, 122
PctI GAATGC 1 cut(s) 557
PfeI GAWTC 7 cut(s) 241, 488, 985, 1025, 1076, 1265, 1469
PkrI GCNGC 6 cut(s) 99, 150, 160, 844, 1054, 1291
PpuMI RGGWCCY 1 cut(s) 848
PsiI TTATAA 1 cut(s) 1226
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 3 cut(s) 270, 1317, 1507
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 3 cut(s) 270, 1317, 1507
PspN4I GGNNCC 6 cut(s) 49, 752, 769, 850, 1153, 1513
PspPI GGNCC 7 cut(s) 156, 246, 434, 750, 848, 934, 1380
PspPPI RGGWCCY 1 cut(s) 848
PstI CTGCAG 1 cut(s) 862
PsuI RGATCY 2 cut(s) 47, 767
PvuII CAGCTG 1 cut(s) 1169
RsaI GTAC 3 cut(s) 384, 1396, 1513
RsaNI GTAC 3 cut(s) 383, 1395, 1512
SaqAI TTAA 4 cut(s) 473, 569, 894, 1666
SatI GCNGC 6 cut(s) 98, 149, 159, 843, 1053, 1290
Sau3AI GATC 5 cut(s) 47, 277, 543, 767, 1643
Sau96I GGNCC 7 cut(s) 156, 246, 434, 750, 848, 934, 1380
ScrFI CCNGG 4 cut(s) 104, 272, 1319, 1509
SduI GDGCHC 3 cut(s) 875, 1240, 1285
SfaNI GCATC 3 cut(s) 489, 727, 1576
SfcI CTRYAG 1 cut(s) 858
SinI GGWCC 4 cut(s) 246, 434, 848, 1380
SmlI CTYRAG 1 cut(s) 654
SmoI CTYRAG 1 cut(s) 654
Sse9I AATT 4 cut(s) 338, 1209, 1566, 1633
SseBI AGGCCT 1 cut(s) 1317
SsiI CCGC 5 cut(s) 41, 45, 149, 159, 929
SspI AATATT 3 cut(s) 522, 891, 1114
SspMI CTAG 4 cut(s) 425, 458, 1161, 1286
StuI AGGCCT 1 cut(s) 1317
StyD4I CCNGG 4 cut(s) 102, 270, 1317, 1507
StyI CCWWGG 2 cut(s) 912, 1408
TaaI ACNGT 3 cut(s) 298, 1260, 1519
TaiI ACGT 2 cut(s) 113, 200
TaqI TCGA 3 cut(s) 167, 179, 276
TaqII GACCGA 1 cut(s) 1085
TasI AATT 4 cut(s) 338, 1209, 1566, 1633
TatI WGTACW 1 cut(s) 1394
TauI GCSGC 2 cut(s) 151, 161
TfiI GAWTC 7 cut(s) 241, 488, 985, 1025, 1076, 1265, 1469
Tru1I TTAA 4 cut(s) 473, 569, 894, 1666
Tru9I TTAA 4 cut(s) 473, 569, 894, 1666
TscAI CASTG 6 cut(s) 514, 714, 862, 1263, 1283, 1522
TseI GCWGC 4 cut(s) 97, 842, 1052, 1289
TspDTI ATGAA 7 cut(s) 583, 735, 812, 1113, 1194, 1482, 1627
TspGWI ACGGA 1 cut(s) 760
TspRI CASTG 6 cut(s) 514, 714, 862, 1263, 1283, 1522
VpaK11BI GGWCC 4 cut(s) 246, 434, 848, 1380
XagI CCTNNNNNAGG 2 cut(s) 1146, 1159
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XspI CTAG 4 cut(s) 425, 458, 1161, 1286
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.