Rh2AG027300

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019816
Physical Location & Seq
Reverse (-)
1902867 .. 1904029
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG027300.1

Sequence Viewer

Length: 864 bp
ATGTATGGCATTGCTGTAGCCGCCCTTGGAATGCTGAGCACCATAGCAACTGGATTGGCCATTGATGCATATGGTCCGATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATCAGAGAGAGAACTGATGCTCTTGATGCTGCAGGAAACACCACGGCTGCTATTGGAAAGGGTTTTGCAATTGGCTCCGCTGCCCTTGTGTCCCTTGCTCTCTTTGGTGCCTTTGTGAGCCGTGCTGGAATTGCAACAGTTGATGTGTTGACTCCAAAAGTGTTCATTGGTTTGATAGTGGGTGCAATGCTTCCCTATTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCTGCTCTAAAGATGGTTGAAGAAGTACGCAGGCAATTCAACACCATCCCTGGCCTTATGGAGGGTACTGCTAAACCTGACTATGCTACTTGCGTCAAGATCTCGACTGATGCTTCCATCAAGGAAATGATCCCTCCTGGTGCCCTTGTCATGCTAACACCCCTCATTGTCGGAATCTTTTTTGGTGTGGAGACTCTCTCTGGTGTCCTTGCTGGATCCCTCGTCTCTGGTGTACAGATCGCCATCTCTGCATCCAACACCGGTGGCGCTTGGGACAATGCCAAGAAGTATATTGAGGCCGGGGCTTCAGAACATGCAAGGACTCTTGGTCCCAAGGGATCAGATCCACACAAAGCAGCTGTCATTGGTGACACCGTCGGAGACCCTCTCAAGGACACATCCGGCCCATCTCTCAACATCCTCATCAAGCTGATGGCTGTCGAGTCCCTAGTGTTTGCACCTTTCTTCGCCACACATGGCGGTCTCCTTTTCAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

287

Amino Acids

29.38

Weight (kDa)

6.42

Isoelectric Point (pI)

15.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 1 - 273 2.1e-128 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019816)

Species Orthologous Gene IDs
pyrus_communis pycom04g17160 pycom12g19450 pycom17g22040
rosa_multiflora Rmu_co7963982.1_g000001 Rmu_sc0024704.1_g000001
rosa_samantha Rh2AG027300 Rh2AG375200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 236, 503
AciI CCGC 3 cut(s) 21, 207, 843
AclWI GGATC 5 cut(s) 487, 573, 586, 701, 709
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 3 cut(s) 390, 430, 597
AfiI CCNNNNNNNGG 2 cut(s) 424, 754
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 3 cut(s) 383, 403, 856
AhdI GACNNNNNGTC 1 cut(s) 690
AjnI CCWGG 2 cut(s) 412, 499
AluBI AGCT 3 cut(s) 722, 793, 859
AluI AGCT 3 cut(s) 722, 793, 859
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 4 cut(s) 548, 592, 738, 851
AlwI GGATC 5 cut(s) 487, 573, 586, 701, 709
AoxI GGCC 4 cut(s) 57, 415, 660, 766
ApeKI GCWGC 5 cut(s) 158, 176, 209, 365, 719
ArsI GACNNNNNNTTYG 2 cut(s) 708, 740
AsiGI ACCGGT 1 cut(s) 623
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 3 cut(s) 74, 692, 767
AsuC2I CCSGG 1 cut(s) 664
AsuHPI GGTGA 1 cut(s) 743
AvaII GGWCC 2 cut(s) 74, 692
BaeGI GKGCMC 1 cut(s) 508
BalI TGGCCA 1 cut(s) 59
BamHI GGATCC 1 cut(s) 578
BanI GGYRCC 2 cut(s) 236, 503
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 5 cut(s) 145, 163, 196, 352, 731
BccI CCATC 7 cut(s) 103, 370, 416, 488, 614, 778, 790
BceAI ACGGC 2 cut(s) 189, 234
BciT130I CCWGG 2 cut(s) 414, 501
BcnI CCSGG 1 cut(s) 664
BcoDI GTCTC 4 cut(s) 548, 592, 738, 851
BfaI CTAG 1 cut(s) 812
BfmI CTRYAG 2 cut(s) 15, 159
BfoI RGCGCY 1 cut(s) 633
BglII AGATCT 1 cut(s) 462
BisI GCNGC 6 cut(s) 21, 159, 177, 210, 366, 720
BlpI GCTNAGC 1 cut(s) 35
BlsI GCNGC 6 cut(s) 22, 160, 178, 211, 367, 721
Bme1390I CCNGG 3 cut(s) 414, 501, 664
Bme18I GGWCC 2 cut(s) 74, 692
BmeRI GACNNNNNGTC 1 cut(s) 690
BmgT120I GGNCC 3 cut(s) 74, 692, 767
BmiI GGNNCC 5 cut(s) 205, 238, 505, 580, 694
BmrFI CCNGG 3 cut(s) 414, 501, 664
BmsI GCATC 5 cut(s) 55, 136, 145, 463, 623
BplI GAGNNNNNCTC 4 cut(s) 545, 577, 735, 767
BpmI CTGGAG 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 664
BsaBI GATNNNNATC 1 cut(s) 605
BsaI GGTCTC 2 cut(s) 738, 851
BsaJI CCNNGG 5 cut(s) 25, 171, 412, 663, 696
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 754
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 1 cut(s) 55
Bse3DI GCAATG 3 cut(s) 9, 99, 321
Bse8I GATNNNNATC 1 cut(s) 605
BseBI CCWGG 2 cut(s) 414, 501
BseDI CCNNGG 5 cut(s) 25, 171, 412, 663, 696
BseGI GGATG 4 cut(s) 408, 614, 761, 780
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 2 cut(s) 424, 754
BseMI GCAATG 3 cut(s) 9, 99, 321
BseMII CTCAG 2 cut(s) 26, 96
BseNI ACTGG 1 cut(s) 55
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 5 cut(s) 145, 163, 196, 352, 731
BshFI GGCC 4 cut(s) 59, 417, 662, 768
BshNI GGYRCC 2 cut(s) 236, 503
BshTI ACCGGT 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 3 cut(s) 624, 663, 765
BslFI GGGAC 4 cut(s) 205, 650, 678, 793
BslI CCNNNNNNNGG 2 cut(s) 424, 754
BsmAI GTCTC 4 cut(s) 548, 592, 738, 851
BsmBI CGTCTC 1 cut(s) 592
BsmFI GGGAC 4 cut(s) 205, 650, 678, 793
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 4 cut(s) 59, 417, 662, 768
Bso31I GGTCTC 2 cut(s) 738, 851
Bsp1286I GDGCHC 2 cut(s) 41, 508
Bsp1407I TGTACA 1 cut(s) 595
Bsp143I GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
Bsp1720I GCTNAGC 1 cut(s) 35
BspACI CCGC 3 cut(s) 21, 207, 843
BspANI GGCC 4 cut(s) 59, 417, 662, 768
BspCNI CTCAG 2 cut(s) 27, 97
BspLI GGNNCC 5 cut(s) 205, 238, 505, 580, 694
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 5 cut(s) 487, 573, 586, 701, 709
BspT107I GGYRCC 2 cut(s) 236, 503
BspTNI GGTCTC 2 cut(s) 738, 851
BsrDI GCAATG 3 cut(s) 9, 99, 321
BsrFI RCCGGY 1 cut(s) 623
BsrGI TGTACA 1 cut(s) 595
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 5 cut(s) 25, 171, 412, 663, 696
BssMI GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
BssT1I CCWWGG 2 cut(s) 25, 696
Bst2UI CCWGG 2 cut(s) 414, 501
Bst4CI ACNGT 2 cut(s) 268, 739
Bst6I CTCTTC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 595
BstC8I GCNNGC 2 cut(s) 115, 395
BstDEI CTNAG 2 cut(s) 35, 105
BstDSI CCRYGG 1 cut(s) 171
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 4 cut(s) 408, 614, 761, 780
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 7 cut(s) 81, 465, 495, 581, 603, 704, 709
BstMAI GTCTC 4 cut(s) 548, 592, 738, 851
BstMBI GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
BstMWI GCNNNNNNNGC 6 cut(s) 20, 65, 110, 155, 260, 611
BstNI CCWGG 2 cut(s) 414, 501
BstNSI RCATGY 1 cut(s) 680
BstSCI CCNGG 3 cut(s) 412, 499, 662
BstSFI CTRYAG 2 cut(s) 15, 159
BstSLI GKGCMC 1 cut(s) 508
BstV1I GCAGC 5 cut(s) 145, 163, 196, 352, 731
BstX2I RGATCY 3 cut(s) 462, 578, 706
BstYI RGATCY 3 cut(s) 462, 578, 706
BsuRI GGCC 4 cut(s) 59, 417, 662, 768
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 4 cut(s) 408, 614, 761, 780
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 2 cut(s) 115, 395
CfoI GCGC 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 3 cut(s) 74, 692, 767
CseI GACGC 1 cut(s) 445
Csp6I GTAC 3 cut(s) 389, 429, 596
CspAI ACCGGT 1 cut(s) 623
CspCI CAANNNNNGTGG 2 cut(s) 607, 642
CviAII CATG 5 cut(s) 118, 340, 514, 677, 839
CviQI GTAC 3 cut(s) 389, 429, 596
DdeI CTNAG 2 cut(s) 35, 105
DpnI GATC 7 cut(s) 80, 464, 494, 580, 602, 703, 708
DpnII GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
DriI GACNNNNNGTC 1 cut(s) 690
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 344
Eam1105I GACNNNNNGTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 25, 696
Eco31I GGTCTC 2 cut(s) 738, 851
Eco47I GGWCC 2 cut(s) 74, 692
Eco57I CTGAAG 1 cut(s) 654
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 412, 499
EcoT14I CCWWGG 2 cut(s) 25, 696
EcoT22I ATGCAT 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 25, 696
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 5 cut(s) 121, 343, 517, 680, 842
FaqI GGGAC 4 cut(s) 205, 650, 678, 793
FatI CATG 5 cut(s) 117, 339, 513, 676, 838
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 6 cut(s) 21, 159, 177, 210, 366, 720
FokI GGATG 4 cut(s) 395, 601, 748, 767
Fsp4HI GCNGC 6 cut(s) 21, 159, 177, 210, 366, 720
FspBI CTAG 1 cut(s) 812
GlaI GCGC 1 cut(s) 631
GluI GCNGC 6 cut(s) 21, 159, 177, 210, 366, 720
GsuI CTGGAG 1 cut(s) 114
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 4 cut(s) 59, 417, 662, 768
HapII CCGG 3 cut(s) 624, 663, 765
HgaI GACGC 1 cut(s) 445
HhaI GCGC 1 cut(s) 632
Hin1II CATG 5 cut(s) 121, 343, 517, 680, 842
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 6 cut(s) 129, 280, 537, 556, 685, 806
HpaII CCGG 3 cut(s) 624, 663, 765
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 2 cut(s) 279, 596
Hpy188I TCNGA 6 cut(s) 78, 134, 536, 673, 706, 743
Hpy188III TCNNGA 3 cut(s) 152, 460, 466
Hpy8I GTNNAC 2 cut(s) 279, 596
Hpy99I CGWCG 1 cut(s) 743
HpyCH4III ACNGT 2 cut(s) 268, 739
HpyCH4V TGCA 8 cut(s) 68, 161, 197, 263, 314, 614, 680, 821
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 65, 110, 155, 260, 611
HpyF3I CTNAG 2 cut(s) 35, 105
Hsp92II CATG 5 cut(s) 121, 343, 517, 680, 842
HspAI GCGC 1 cut(s) 630
Kzo9I GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 5 cut(s) 145, 163, 196, 352, 731
LweI GCATC 5 cut(s) 55, 136, 145, 463, 623
MaeI CTAG 1 cut(s) 812
MaeIII GTNAC 2 cut(s) 83, 731
MalI GATC 7 cut(s) 80, 464, 494, 580, 602, 703, 708
MboI GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
MboII GAAGA 3 cut(s) 361, 395, 820
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 3 cut(s) 462, 578, 706
MhlI GDGCHC 2 cut(s) 41, 508
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 3 cut(s) 198, 258, 398
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 4 cut(s) 274, 550, 679, 815
MmeI TCCRAC 3 cut(s) 514, 642, 721
MnlI CCTC 8 cut(s) 89, 418, 507, 536, 593, 652, 759, 794
Mox20I TGGCCA 1 cut(s) 59
Mph1103I ATGCAT 1 cut(s) 70
MscI TGGCCA 1 cut(s) 59
MseI TTAA 1 cut(s) 862
MslI CAYNNNNRTG 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 2 cut(s) 209, 722
MspI CCGG 3 cut(s) 624, 663, 765
MspR9I CCNGG 3 cut(s) 414, 501, 664
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 2 cut(s) 414, 501
MwoI GCNNNNNNNGC 6 cut(s) 20, 65, 110, 155, 260, 611
NciI CCSGG 1 cut(s) 664
NdeI CATATG 1 cut(s) 70
NdeII GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
NlaIII CATG 5 cut(s) 121, 343, 517, 680, 842
NlaIV GGNNCC 5 cut(s) 205, 238, 505, 580, 694
NmuCI GTSAC 2 cut(s) 83, 731
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 680
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 129, 537
PflFI GACNNNGTC 1 cut(s) 737
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 6 cut(s) 22, 160, 178, 211, 367, 721
PleI GAGTC 4 cut(s) 274, 550, 679, 814
PpsI GAGTC 4 cut(s) 274, 550, 679, 814
Psp6I CCWGG 2 cut(s) 412, 499
PspGI CCWGG 2 cut(s) 412, 499
PspN4I GGNNCC 5 cut(s) 205, 238, 505, 580, 694
PspPI GGNCC 3 cut(s) 74, 692, 767
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 3 cut(s) 462, 578, 706
PsyI GACNNNGTC 1 cut(s) 737
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 3 cut(s) 390, 430, 597
RsaNI GTAC 3 cut(s) 389, 429, 596
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 862
SatI GCNGC 6 cut(s) 21, 159, 177, 210, 366, 720
Sau3AI GATC 7 cut(s) 78, 462, 492, 578, 600, 701, 706
Sau96I GGNCC 3 cut(s) 74, 692, 767
SchI GAGTC 4 cut(s) 274, 550, 679, 815
ScrFI CCNGG 3 cut(s) 414, 501, 664
SduI GDGCHC 2 cut(s) 41, 508
SetI ASST 5 cut(s) 442, 724, 795, 826, 861
SfaNI GCATC 5 cut(s) 55, 136, 145, 463, 623
SfcI CTRYAG 2 cut(s) 15, 159
SgrAI CRCCGGYG 1 cut(s) 623
SinI GGWCC 2 cut(s) 74, 692
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 752
SmoI CTYRAG 1 cut(s) 752
Sse9I AATT 3 cut(s) 198, 258, 398
SsiI CCGC 3 cut(s) 21, 207, 843
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 3 cut(s) 412, 499, 662
StyI CCWWGG 2 cut(s) 25, 696
TaaI ACNGT 2 cut(s) 268, 739
TaqI TCGA 2 cut(s) 467, 804
TasI AATT 3 cut(s) 198, 258, 398
TatI WGTACW 1 cut(s) 595
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 2 cut(s) 129, 537
Tru1I TTAA 1 cut(s) 862
Tru9I TTAA 1 cut(s) 862
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 2 cut(s) 83, 731
TseI GCWGC 5 cut(s) 158, 176, 209, 365, 719
Tsp45I GTSAC 2 cut(s) 83, 731
TspDTI ATGAA 2 cut(s) 283, 362
TspRI CASTG 1 cut(s) 88
Tth111I GACNNNGTC 1 cut(s) 737
VpaK11BI GGWCC 2 cut(s) 74, 692
XagI CCTNNNNNAGG 1 cut(s) 422
XceI RCATGY 1 cut(s) 680
XspI CTAG 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.