Rh2AG196100

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019925
Physical Location & Seq
Reverse (-)
19616045 .. 19617528
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG196100.1

Sequence Viewer

Length: 654 bp
ATGGTCCAAAAGCCTAGTTCTGTTGTTGGATCTACTGGTTCCATTGGAACCCAGACATTGGACATTGTTGCAGAGAACCCGAAAAAAATCAGAGTTGTGGCCCTAGTAGCTAGCTCAAATGTGTATCTTCTTGTTGATCAGTTATATGTATCAGGAAAAGCCTTCTCCCGTCTTTTGAGGGATTCAAAGAATAAAGCTGGTCAAGGAGGCCGTGGGGGTGGAAGTGGATATAGATATGGGCGAGGCCGTGGCGGTGGTAGACACAATTGTGATTCAGCCAATAATGAAAACAACAGTGGGTACAATCGTGAATCAGCCCCTGCTGGTCAGGGTGCTTTTAAGGATGCACCTCCTAAACGGCGTTTCTCTGGTGGGGATGGTGATGGAAGGCCATTTGAAAGGCGTGGCTATGTAACTTACTTTATAATGAATCCTACAAGGCTTGCATTCATTCGCTCAACACTGTGCTGTCATTTCAGGAGGGACCCATTATCTGCTAGGCCCTTTGAAGGGCTCAAATTTATTGATGTTGGCTGTGGAGGTGGAATTCTTTCTGAGCCTTTAGCTCGGATGAGAGCTGCTGTAACAGGAGTTGATGCTGTTAAGAAAAATATCAAGATAGCTCGCTTTCACTCAGTATGGTCATTCTCTTAA

Protein Analysis

217

Amino Acids

23.39

Weight (kDa)

10.31

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_reductoisom PF02670 8 - 48 1.3e-11 1-deoxy-D-xylulose 5-phosphate reductoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019925)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0436731
rosa_samantha Rh2AG196100 Rh2DG203800 Rh4AG350100 Rh4DG353000 Rh6CG162700 Rh6DG154300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 58
AccI GTMKAC 1 cut(s) 259
AciI CCGC 1 cut(s) 252
AclWI GGATC 1 cut(s) 37
AcsI RAATTY 2 cut(s) 518, 546
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 58, 509, 510
AgsI TTSAA 3 cut(s) 186, 398, 509
AleI CACNNNNGTG 1 cut(s) 267
AluBI AGCT 6 cut(s) 110, 114, 197, 566, 578, 623
AluI AGCT 6 cut(s) 110, 114, 197, 566, 578, 623
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 5 cut(s) 99, 208, 244, 389, 500
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 2 cut(s) 518, 546
Asp700I GAANNNNTTC 1 cut(s) 550
AspS9I GGNCC 4 cut(s) 4, 100, 484, 501
AsuHPI GGTGA 1 cut(s) 392
AsuNHI GCTAGC 1 cut(s) 110
AvaII GGWCC 2 cut(s) 4, 484
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BanII GRGCYC 1 cut(s) 516
BbvI GCAGC 1 cut(s) 565
BccI CCATC 2 cut(s) 371, 377
BceAI ACGGC 3 cut(s) 195, 231, 374
BclI TGATCA 1 cut(s) 136
BfaI CTAG 4 cut(s) 15, 104, 111, 498
BisI GCNGC 1 cut(s) 579
BlsI GCNGC 1 cut(s) 580
Bme18I GGWCC 2 cut(s) 4, 484
BmgT120I GGNCC 4 cut(s) 4, 100, 484, 501
BmiI GGNNCC 4 cut(s) 40, 49, 485, 486
BmsI GCATC 2 cut(s) 334, 586
BmtI GCTAGC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 211, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 509, 510
Bse1I ACTGG 1 cut(s) 40
BseDI CCNNGG 2 cut(s) 211, 247
BseGI GGATG 3 cut(s) 349, 382, 576
BseLI CCNNNNNNNGG 3 cut(s) 58, 509, 510
BseMII CTCAG 2 cut(s) 546, 648
BseNI ACTGG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 565
BshFI GGCC 5 cut(s) 101, 210, 246, 391, 502
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 58, 509, 510
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 5 cut(s) 101, 210, 246, 391, 502
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 2 cut(s) 29, 136
BspACI CCGC 1 cut(s) 252
BspANI GGCC 5 cut(s) 101, 210, 246, 391, 502
BspCNI CTCAG 2 cut(s) 547, 647
BspLI GGNNCC 4 cut(s) 40, 49, 485, 486
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 37
BsrI ACTGG 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 211, 247
BssMI GATC 2 cut(s) 29, 136
Bst4CI ACNGT 2 cut(s) 296, 465
BstC8I GCNNGC 3 cut(s) 112, 444, 625
BstDEI CTNAG 2 cut(s) 555, 634
BstDSI CCRYGG 2 cut(s) 211, 247
BstF5I GGATG 3 cut(s) 349, 382, 576
BstKTI GATC 2 cut(s) 32, 139
BstMBI GATC 2 cut(s) 29, 136
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BsuRI GGCC 5 cut(s) 101, 210, 246, 391, 502
BtgI CCRYGG 2 cut(s) 211, 247
BtsCI GGATG 3 cut(s) 349, 382, 576
BtsIMutI CAGTG 2 cut(s) 301, 461
Cac8I GCNNGC 3 cut(s) 112, 444, 625
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 4 cut(s) 4, 100, 484, 501
Csp6I GTAC 1 cut(s) 301
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 2 cut(s) 555, 634
DpnI GATC 2 cut(s) 31, 138
DpnII GATC 2 cut(s) 29, 136
Eco24I GRGCYC 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 4, 484
EcoO109I RGGNCCY 2 cut(s) 484, 501
EcoRI GAATTC 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 516
FaiI YATR 7 cut(s) 145, 147, 231, 237, 411, 425, 640
FaqI GGGAC 1 cut(s) 497
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 3 cut(s) 356, 389, 583
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 1 cut(s) 579
FspBI CTAG 4 cut(s) 15, 104, 111, 498
GluI GCNGC 1 cut(s) 579
HaeIII GGCC 5 cut(s) 101, 210, 246, 391, 502
HinfI GANTC 4 cut(s) 182, 272, 311, 430
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 3 cut(s) 92, 556, 570
Hpy188III TCNNGA 4 cut(s) 153, 308, 478, 616
Hpy8I GTNNAC 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 172, 381, 503
HpyCH4III ACNGT 2 cut(s) 296, 465
HpyCH4V TGCA 3 cut(s) 71, 347, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 555, 634
KflI GGGWCCC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 29, 136
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 2 cut(s) 334, 586
MaeI CTAG 4 cut(s) 15, 104, 111, 498
MaeIII GTNAC 2 cut(s) 412, 583
MalI GATC 2 cut(s) 31, 138
MboI GATC 2 cut(s) 29, 136
MboII GAAGA 1 cut(s) 119
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 516
MluCI AATT 3 cut(s) 265, 518, 546
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 6 cut(s) 171, 200, 236, 360, 474, 533
MroXI GAANNNNTTC 1 cut(s) 550
MseI TTAA 3 cut(s) 339, 603, 652
MslI CAYNNNNRTG 1 cut(s) 267
MunI CAATTG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 2 cut(s) 29, 136
NheI GCTAGC 1 cut(s) 110
NlaIV GGNNCC 4 cut(s) 40, 49, 485, 486
OliI CACNNNNGTG 1 cut(s) 267
PctI GAATGC 1 cut(s) 446
PdmI GAANNNNTTC 1 cut(s) 550
PfeI GAWTC 4 cut(s) 182, 272, 311, 430
PflMI CCANNNNNTGG 1 cut(s) 58
PkrI GCNGC 1 cut(s) 580
PpuMI RGGWCCY 1 cut(s) 484
PsiI TTATAA 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 484
PspN4I GGNNCC 4 cut(s) 40, 49, 485, 486
PspPI GGNCC 4 cut(s) 4, 100, 484, 501
PspPPI RGGWCCY 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 3 cut(s) 339, 603, 652
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 2 cut(s) 29, 136
Sau96I GGNCC 4 cut(s) 4, 100, 484, 501
SduI GDGCHC 1 cut(s) 516
SetI ASST 8 cut(s) 112, 116, 199, 352, 544, 568, 580, 625
SfaNI GCATC 2 cut(s) 334, 586
SinI GGWCC 2 cut(s) 4, 484
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 3 cut(s) 265, 518, 546
SsiI CCGC 1 cut(s) 252
SspMI CTAG 4 cut(s) 15, 104, 111, 498
TaaI ACNGT 2 cut(s) 296, 465
TasI AATT 3 cut(s) 265, 518, 546
TfiI GAWTC 4 cut(s) 182, 272, 311, 430
Tru1I TTAA 3 cut(s) 339, 603, 652
Tru9I TTAA 3 cut(s) 339, 603, 652
TscAI CASTG 2 cut(s) 301, 468
TseI GCWGC 1 cut(s) 578
TspDTI ATGAA 3 cut(s) 300, 439, 443
TspRI CASTG 2 cut(s) 301, 468
Van91I CCANNNNNTGG 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 4, 484
XapI RAATTY 2 cut(s) 518, 546
XmiI GTMKAC 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 550
XspI CTAG 4 cut(s) 15, 104, 111, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.