Rh6CG162700

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0019925
Physical Location & Seq
Forward (+)
22010458 .. 22018319
7862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG162700.1

Sequence Viewer

Length: 666 bp
ATGGTCCAAAAGCCTAGTTCTGTTGTTGGATCTACTGGTTCCATTGGAACCCAGACATTGGACATTGTTGCAGAGAACCCGAAAAAATTCAGAGTTGTGGCCCTAGTAGCTGGCTCAAATGTGTATCTTCTTGTTGATCAGTTATATGTATCAGGAAAAGCCTTCTCCCGTCTTTTGAGGGATTCAAAGAATAAAGCTGGTCAAGGAGGCCGTGGGGGTGGAAGTGGATATGGATATGGGCGAGGCCGTGGCGGTGGTAGATACAATTGTGATTCAGCCAATAATGAAAACAACAGTGGGTACAATCGTGAATCAGCCCCTGCTGGTCAGGGTGCTTTTAAGGATGCACCTCCTAAACGGCGTTTCTCTGGTGGGGATGGTGATGGAAGGCCATTTGAAAGGCGTGACTATGTAACTTACTTTATAATGAATCCTACAAGGCTTGCATTCATTCGCTCAACACTGTGCCGTCATTTCAGGAGGGACCCATTATCTGCTAGGCCCTTTGAAGGGCTCAAATTTATTGATGTTGGCTGTGGAGGTGGAATTCTTTCTAAGGACTTGGATCCAGTGACCTCAAAACTTGAATGTCAATGCACTACAGCTGTGTCAAATATATTAATCAATGAAGAAGATTACAATCGGATCGATGCTAAAGCATTTTGA

Protein Analysis

221

Amino Acids

23.91

Weight (kDa)

9.51

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_reductoisom PF02670 8 - 48 1.5e-12 1-deoxy-D-xylulose 5-phosphate reductoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019925)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0436731
rosa_samantha Rh2AG196100 Rh2DG203800 Rh4AG350100 Rh4DG353000 Rh6CG162700 Rh6DG154300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 58
AciI CCGC 1 cut(s) 252
AclWI GGATC 4 cut(s) 37, 560, 573, 653
AcsI RAATTY 3 cut(s) 86, 518, 546
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 58, 509, 510
AgsI TTSAA 4 cut(s) 186, 398, 509, 587
AluBI AGCT 3 cut(s) 110, 197, 605
AluI AGCT 3 cut(s) 110, 197, 605
AlwI GGATC 4 cut(s) 37, 560, 573, 653
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 5 cut(s) 99, 208, 244, 389, 500
ApoI RAATTY 3 cut(s) 86, 518, 546
AseI ATTAAT 1 cut(s) 620
Asp700I GAANNNNTTC 2 cut(s) 86, 550
AspS9I GGNCC 4 cut(s) 4, 100, 484, 501
AsuHPI GGTGA 1 cut(s) 392
AvaII GGWCC 2 cut(s) 4, 484
BaeI ACNNNNGTAYC 4 cut(s) 253, 286, 292, 325
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 516
BccI CCATC 2 cut(s) 371, 377
BceAI ACGGC 4 cut(s) 195, 231, 374, 453
BclI TGATCA 1 cut(s) 136
BfaI CTAG 3 cut(s) 15, 104, 498
BfmI CTRYAG 1 cut(s) 600
Bme18I GGWCC 2 cut(s) 4, 484
BmgT120I GGNCC 4 cut(s) 4, 100, 484, 501
BmiI GGNNCC 5 cut(s) 40, 49, 485, 486, 567
BmsI GCATC 2 cut(s) 334, 640
Bsa29I ATCGAT 1 cut(s) 648
BsaBI GATNNNNATC 1 cut(s) 639
BsaJI CCNNGG 2 cut(s) 211, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 509, 510
Bse1I ACTGG 2 cut(s) 40, 569
Bse8I GATNNNNATC 1 cut(s) 639
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 2 cut(s) 211, 247
BseGI GGATG 2 cut(s) 349, 382
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 3 cut(s) 58, 509, 510
BseNI ACTGG 2 cut(s) 40, 569
BshFI GGCC 5 cut(s) 101, 210, 246, 391, 502
BshVI ATCGAT 1 cut(s) 648
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 58, 509, 510
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 5 cut(s) 101, 210, 246, 391, 502
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 4 cut(s) 29, 136, 565, 645
BspACI CCGC 1 cut(s) 252
BspANI GGCC 5 cut(s) 101, 210, 246, 391, 502
BspDI ATCGAT 1 cut(s) 648
BspLI GGNNCC 5 cut(s) 40, 49, 485, 486, 567
BspPI GGATC 4 cut(s) 37, 560, 573, 653
BsrI ACTGG 2 cut(s) 40, 569
BssECI CCNNGG 2 cut(s) 211, 247
BssMI GATC 4 cut(s) 29, 136, 565, 645
Bst4CI ACNGT 2 cut(s) 296, 465
BstC8I GCNNGC 2 cut(s) 112, 444
BstDEI CTNAG 1 cut(s) 555
BstDSI CCRYGG 2 cut(s) 211, 247
BstF5I GGATG 2 cut(s) 349, 382
BstKTI GATC 4 cut(s) 32, 139, 568, 648
BstMBI GATC 4 cut(s) 29, 136, 565, 645
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 600
BstX2I RGATCY 2 cut(s) 29, 565
BstYI RGATCY 2 cut(s) 29, 565
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 5 cut(s) 101, 210, 246, 391, 502
BsuTUI ATCGAT 1 cut(s) 648
BtgI CCRYGG 2 cut(s) 211, 247
BtsCI GGATG 2 cut(s) 349, 382
BtsIMutI CAGTG 3 cut(s) 301, 461, 576
Cac8I GCNNGC 2 cut(s) 112, 444
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 4 cut(s) 4, 100, 484, 501
ClaI ATCGAT 1 cut(s) 648
Csp6I GTAC 1 cut(s) 301
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 1 cut(s) 555
DpnI GATC 4 cut(s) 31, 138, 567, 647
DpnII GATC 4 cut(s) 29, 136, 565, 645
Eco24I GRGCYC 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 4, 484
EcoO109I RGGNCCY 2 cut(s) 484, 501
EcoRI GAATTC 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 516
FaiI YATR 7 cut(s) 145, 147, 231, 237, 411, 425, 617
FaqI GGGAC 1 cut(s) 497
FbaI TGATCA 1 cut(s) 136
FokI GGATG 2 cut(s) 356, 389
FriOI GRGCYC 1 cut(s) 516
FspBI CTAG 3 cut(s) 15, 104, 498
HaeIII GGCC 5 cut(s) 101, 210, 246, 391, 502
HinfI GANTC 4 cut(s) 182, 272, 311, 430
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 2 cut(s) 92, 645
Hpy188III TCNNGA 3 cut(s) 153, 308, 478
HpyAV CCTTC 3 cut(s) 172, 381, 503
HpyCH4III ACNGT 2 cut(s) 296, 465
HpyCH4V TGCA 4 cut(s) 71, 347, 446, 597
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 555
KflI GGGWCCC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 4 cut(s) 29, 136, 565, 645
LweI GCATC 2 cut(s) 334, 640
MaeI CTAG 3 cut(s) 15, 104, 498
MaeIII GTNAC 3 cut(s) 404, 412, 571
MalI GATC 4 cut(s) 31, 138, 567, 647
MboI GATC 4 cut(s) 29, 136, 565, 645
MboII GAAGA 3 cut(s) 119, 641, 644
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 2 cut(s) 29, 565
MhlI GDGCHC 1 cut(s) 516
MluCI AATT 4 cut(s) 86, 265, 518, 546
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 7 cut(s) 171, 200, 236, 360, 474, 533, 586
MroXI GAANNNNTTC 2 cut(s) 86, 550
MseI TTAA 2 cut(s) 339, 620
MspA1I CMGCKG 1 cut(s) 605
MunI CAATTG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 4 cut(s) 29, 136, 565, 645
NlaIV GGNNCC 5 cut(s) 40, 49, 485, 486, 567
NmuCI GTSAC 2 cut(s) 404, 571
PctI GAATGC 1 cut(s) 446
PdmI GAANNNNTTC 2 cut(s) 86, 550
PfeI GAWTC 4 cut(s) 182, 272, 311, 430
PflMI CCANNNNNTGG 1 cut(s) 58
PpuMI RGGWCCY 1 cut(s) 484
PshBI ATTAAT 1 cut(s) 620
PsiI TTATAA 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 484
PspN4I GGNNCC 5 cut(s) 40, 49, 485, 486, 567
PspPI GGNCC 4 cut(s) 4, 100, 484, 501
PspPPI RGGWCCY 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 2 cut(s) 29, 565
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 339, 620
Sau3AI GATC 4 cut(s) 29, 136, 565, 645
Sau96I GGNCC 4 cut(s) 4, 100, 484, 501
SduI GDGCHC 1 cut(s) 516
SetI ASST 6 cut(s) 112, 199, 352, 544, 578, 607
SfaNI GCATC 2 cut(s) 334, 640
SfcI CTRYAG 1 cut(s) 600
SinI GGWCC 2 cut(s) 4, 484
Sse9I AATT 4 cut(s) 86, 265, 518, 546
SsiI CCGC 1 cut(s) 252
SspMI CTAG 3 cut(s) 15, 104, 498
TaaI ACNGT 2 cut(s) 296, 465
TaqI TCGA 1 cut(s) 648
TasI AATT 4 cut(s) 86, 265, 518, 546
TfiI GAWTC 4 cut(s) 182, 272, 311, 430
Tru1I TTAA 2 cut(s) 339, 620
Tru9I TTAA 2 cut(s) 339, 620
TscAI CASTG 3 cut(s) 301, 468, 576
TseFI GTSAC 2 cut(s) 404, 571
Tsp45I GTSAC 2 cut(s) 404, 571
TspDTI ATGAA 4 cut(s) 300, 439, 443, 642
TspRI CASTG 3 cut(s) 301, 468, 576
Van91I CCANNNNNTGG 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 4, 484
VspI ATTAAT 1 cut(s) 620
XapI RAATTY 3 cut(s) 86, 518, 546
XmnI GAANNNNTTC 2 cut(s) 86, 550
XspI CTAG 3 cut(s) 15, 104, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.