Rh2AG285200

Chromatin assembly factor 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011488
Physical Location & Seq
Reverse (-)
34809447 .. 34815481
6035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG285200.1

Sequence Viewer

Length: 1209 bp
ATGAAAGGCGGGACAGTCCAGATCAACTGGCACGACACCAAGCCCGTCCTAACCCTAGATTTCCACCCTCATTCCGGCATTCTCGCCACCGGCGGCGCCGATTACGACATCAAGCTTTGGTTGATAAATTCCGGCGAGAAGCTCCCTTCAGTGTCGTATCATAATAGCCTTTCTTACCATAGTTCTGCTATAAATGTCCTTCGCTTCTCTCCTTCTGGAGAACAAATTGCCTCCGGTGCAGATGGAGGCGAGCTAATTATATGGAAGTTGCATGCAACAGAAAATAGTCAAACGTGGAAGGTTCTTAAGACATTGTCATTTCACCGGAAAGACGTGCTGGACCTTGAGTGGTCTGCTGATGGTTCATTTCTCATCTCTGGTTCAGTCGATAATTCATGCATCATATGGGATGCTAACAAAGGTTCTGTCCATCAGATTTTGGATTCCCATTTCCATTATGTTCAAGGTGTGGCATGGGACCCATTGGCCAAGTATGTTGCCTCCCTTAGTTCAGATAGAACTTGTCGCATTTATCTTAAAAAAGCTCAAACAAAAACCAAGGGTGCCGATAAGACAAATTATGTTTGTCAACATGTTATTTCAAAGGCTGAAAACCCTTCAGATGAGTCTAAGTCTGCGAAAAACCATCTCTTTCACGATGAGACACTTCCATCTTTTTTCCGGAGGTTAGCGTGGTCACCTGATGGATCATTTCTACTTGTTCCAGCAGGTTCCTACAAAGTATCACCTGCAACAGAAACAATAAATACTGCTTATGTATTTTCAAGAAAGGACCTTTCAAGGCCTGCTCTACATCTCCCTTGCGCCAGCAAACCAATAGTAGCAGTTCGATTCTGTCCTTTGGCTTTTAGACTGCGGGGAACCAATCCATCGGGGTTCTTTAAGCTTCCCCATCGTATAGTTTTTGCGGTGGCCACTTTAGATTCTCTGAACATTTATGACACAGAGAGTGTTACTCCAATTGCAGTTTGGGCTGGTCTTCATTATGCAGCCATAACAGACATCACATGGTCACCTGATGCCCATTATCTAGCATTATCTTCCCAAGATGGTTACTGCACTTTAGTAGAATTTGAAAATGATGAACTGGGATCACCCATCTATTTGTCAGGTTTGCTGTCCTTATTTTCTATTTGTTTTTCATCACATTTTGTTTATGGATTGTTTCTCCAACTGTTTGGTTCCTAA

Protein Analysis

402

Amino Acids

44.46

Weight (kDa)

6.64

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 1 - 370 2.9e-117 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 9 - 181 1.7e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 11 - 93 4.8e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 12 - 101 8e-10 THOC3 beta-propeller domain
Beta-prop_EML PF23409 12 - 145 5.7e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_IFT140_1st PF23383 12 - 97 2e-07 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 13 - 184 1.7e-28 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 13 - 188 2.1e-12 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 13 - 161 5e-15 WDHD1 first WD40 domain
WD40_Prp19 PF24814 13 - 100 1.1e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 14 - 138 5.7e-11 WDR3 second beta-propeller domain
EIF3I PF24805 16 - 184 1.3e-11 EIF3I
Beta-prop_EML_2 PF23414 18 - 185 3.3e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR75_1st PF23869 19 - 95 5.9e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 27 - 184 8.9e-08 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 61 - 201 4.1e-08 MABP1/WDR62 second WD40 domain
WDR55 PF24796 62 - 148 9.5e-09 WDR55
Beta-prop_IFT122_1st PF23381 63 - 191 1.1e-06 IFT122 first beta-propeller
ANAPC4_WD40 PF12894 68 - 159 9.8e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_CDC20-Fz PF24807 96 - 204 2.3e-12 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 97 - 184 4.4e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 97 - 196 2.8e-12 WDR3 first beta-propeller domain
WD40 PF00400 99 - 137 2e-08 WD domain, G-beta repeat
WD40_Gbeta PF25391 104 - 196 8.1e-08 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 108 - 190 1.1e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64630 AT5G64630 AT5G64630
fragaria_vesca FvH4_1g24150 FvH4_1g24150 FvH4_1g24150 FvH4_1g24150
malus_domestica MD01G1002400.v1.1
prunus_persica Prupe.6G159400_v2.0.a1
pyrus_communis pycom01g02500
rosa_chinensis RchiOBHm_Chr2g0120381
rosa_laevigata RLG00000018554
rosa_multiflora Rmu_sc0005082.1_g000015
rosa_roxburghii Rroxscaffold_2G00123450
rosa_rugosa Rorug02G0229200
rosa_samantha Rh2AG285200 Rh2BG296300 Rh2CG274000 Rh2DG310200
rosa_wichuraiana Rw2G022890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 757
Acc36I ACCTGC 2 cut(s) 719, 757
AccB1I GGYRCC 2 cut(s) 95, 563
AccIII TCCGGA 1 cut(s) 681
AciI CCGC 4 cut(s) 9, 93, 877, 929
AclWI GGATC 2 cut(s) 715, 1120
AcoI YGGCCR 2 cut(s) 486, 933
AcsI RAATTY 2 cut(s) 127, 1091
AcuI CTGAAG 2 cut(s) 132, 603
AcyI GRCGYC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 74
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 5 cut(s) 464, 603, 786, 801, 1097
AjiI CACGTC 1 cut(s) 334
AluBI AGCT 5 cut(s) 115, 142, 253, 545, 907
AluI AGCT 5 cut(s) 115, 142, 253, 545, 907
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 2 cut(s) 715, 1120
Aor13HI TCCGGA 1 cut(s) 681
AoxI GGCC 3 cut(s) 486, 803, 933
ApeKI GCWGC 1 cut(s) 1010
ApoI RAATTY 2 cut(s) 127, 1091
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AspLEI GCGC 2 cut(s) 98, 827
AspS9I GGNCC 3 cut(s) 340, 478, 793
AsuHPI GGTGA 5 cut(s) 314, 690, 738, 1026, 1107
AvaII GGWCC 3 cut(s) 340, 478, 793
BalI TGGCCA 2 cut(s) 488, 935
BanI GGYRCC 2 cut(s) 95, 563
BbsI GAAGAC 1 cut(s) 992
BbvI GCAGC 1 cut(s) 1022
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 2 cut(s) 56, 1052
BfoI RGCGCY 1 cut(s) 99
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 2 cut(s) 719, 757
BisI GCNGC 2 cut(s) 94, 1011
BlsI GCNGC 2 cut(s) 95, 1012
Bme18I GGWCC 3 cut(s) 340, 478, 793
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 340, 478, 793
BmiI GGNNCC 7 cut(s) 97, 479, 480, 565, 733, 883, 1204
BmrI ACTGGG 1 cut(s) 1118
BmsI GCATC 3 cut(s) 400, 408, 1030
BmuI ACTGGG 1 cut(s) 1118
BpiI GAAGAC 1 cut(s) 992
BplI GAGNNNNNCTC 2 cut(s) 961, 993
BpmI CTGGAG 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 558
BsaWI WCCGGW 3 cut(s) 233, 324, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 32, 1113
BseAI TCCGGA 1 cut(s) 681
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 74
BseNI ACTGG 2 cut(s) 32, 1113
BseXI GCAGC 1 cut(s) 1022
BsgI GTGCAG 2 cut(s) 258, 1063
BshFI GGCC 3 cut(s) 488, 805, 935
BshNI GGYRCC 2 cut(s) 95, 563
BsiSI CCGG 6 cut(s) 75, 90, 132, 234, 325, 682
BslFI GGGAC 2 cut(s) 25, 491
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 2 cut(s) 25, 491
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 3 cut(s) 488, 805, 935
Bsp13I TCCGGA 1 cut(s) 681
Bsp143I GATC 3 cut(s) 21, 707, 1112
BspACI CCGC 4 cut(s) 9, 93, 877, 929
BspANI GGCC 3 cut(s) 488, 805, 935
BspEI TCCGGA 1 cut(s) 681
BspLI GGNNCC 7 cut(s) 97, 479, 480, 565, 733, 883, 1204
BspMI ACCTGC 2 cut(s) 719, 757
BspPI GGATC 2 cut(s) 715, 1120
BspT107I GGYRCC 2 cut(s) 95, 563
BspTI CTTAAG 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 2 cut(s) 32, 1113
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 3 cut(s) 21, 707, 1112
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 558
Bst4CI ACNGT 2 cut(s) 16, 1197
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 251, 273, 807, 829
BstDEI CTNAG 2 cut(s) 506, 630
BstEII GGTNACC 2 cut(s) 696, 1032
BstF5I GGATG 1 cut(s) 415
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 827
BstKTI GATC 3 cut(s) 24, 710, 1115
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 3 cut(s) 21, 707, 1112
BstMWI GCNNNNNNNGC 2 cut(s) 236, 992
BstNSI RCATGY 2 cut(s) 275, 596
BstPI GGTNACC 2 cut(s) 696, 1032
BstV1I GCAGC 1 cut(s) 1022
BstV2I GAAGAC 1 cut(s) 992
BstXI CCANNNNNNTGG 1 cut(s) 1199
BsuRI GGCC 3 cut(s) 488, 805, 935
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 156
BveI ACCTGC 2 cut(s) 719, 757
Cac8I GCNNGC 4 cut(s) 251, 273, 807, 829
CfoI GCGC 2 cut(s) 98, 827
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 3 cut(s) 340, 478, 793
CviAII CATG 5 cut(s) 272, 396, 474, 593, 1029
DdeI CTNAG 2 cut(s) 506, 630
DinI GGCGCC 1 cut(s) 97
DpnI GATC 3 cut(s) 23, 709, 1114
DpnII GATC 3 cut(s) 21, 707, 1112
EaeI YGGCCR 2 cut(s) 486, 933
Eco130I CCWWGG 1 cut(s) 558
Eco147I AGGCCT 1 cut(s) 805
Eco47I GGWCC 3 cut(s) 340, 478, 793
Eco57I CTGAAG 2 cut(s) 132, 603
Eco91I GGTNACC 2 cut(s) 696, 1032
EcoO109I RGGNCCY 2 cut(s) 478, 793
EcoO65I GGTNACC 2 cut(s) 696, 1032
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 1 cut(s) 401
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 5 cut(s) 275, 399, 477, 596, 1032
FaqI GGGAC 2 cut(s) 25, 491
FatI CATG 5 cut(s) 271, 395, 473, 592, 1028
FauI CCCGC 2 cut(s) 2, 870
FauNDI CATATG 1 cut(s) 404
Fnu4HI GCNGC 2 cut(s) 94, 1011
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 94, 1011
FspBI CTAG 2 cut(s) 56, 1052
GlaI GCGC 2 cut(s) 97, 826
GluI GCNGC 2 cut(s) 94, 1011
GsuI CTGGAG 1 cut(s) 237
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 3 cut(s) 488, 805, 935
HapII CCGG 6 cut(s) 75, 90, 132, 234, 325, 682
HhaI GCGC 2 cut(s) 98, 827
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 5 cut(s) 275, 399, 477, 596, 1032
Hin6I GCGC 2 cut(s) 96, 825
HinP1I GCGC 2 cut(s) 96, 825
HincII GTYRAC 1 cut(s) 590
HindII GTYRAC 1 cut(s) 590
HindIII AAGCTT 2 cut(s) 113, 905
HinfI GANTC 4 cut(s) 443, 626, 852, 944
HpaII CCGG 6 cut(s) 75, 90, 132, 234, 325, 682
HphI GGTGA 5 cut(s) 314, 690, 738, 1026, 1107
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 4 cut(s) 435, 514, 622, 951
Hpy188III TCNNGA 5 cut(s) 19, 216, 656, 682, 786
Hpy8I GTNNAC 1 cut(s) 590
HpyAV CCTTC 5 cut(s) 156, 209, 222, 292, 627
HpyCH4III ACNGT 2 cut(s) 16, 1197
HpyCH4IV ACGT 2 cut(s) 293, 333
HpyCH4V TGCA 8 cut(s) 239, 271, 275, 399, 752, 986, 1010, 1080
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 992
HpyF3I CTNAG 2 cut(s) 506, 630
HpySE526I ACGT 2 cut(s) 293, 333
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 5 cut(s) 275, 399, 477, 596, 1032
HspAI GCGC 2 cut(s) 96, 825
KasI GGCGCC 1 cut(s) 95
KflI GGGWCCC 1 cut(s) 478
Kpn2I TCCGGA 1 cut(s) 681
Kzo9I GATC 3 cut(s) 21, 707, 1112
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 1 cut(s) 1022
LweI GCATC 3 cut(s) 400, 408, 1030
MaeI CTAG 2 cut(s) 56, 1052
MaeII ACGT 2 cut(s) 293, 333
MaeIII GTNAC 4 cut(s) 696, 973, 1032, 1073
MalI GATC 3 cut(s) 23, 709, 1114
MboI GATC 3 cut(s) 21, 707, 1112
MboII GAAGA 2 cut(s) 992, 1053
MfeI CAATTG 1 cut(s) 981
MlsI TGGCCA 2 cut(s) 488, 935
MluCI AATT 7 cut(s) 127, 225, 255, 391, 577, 981, 1091
MluNI TGGCCA 2 cut(s) 488, 935
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 5 cut(s) 78, 239, 241, 511, 678
Mox20I TGGCCA 2 cut(s) 488, 935
Mph1103I ATGCAT 1 cut(s) 401
MroI TCCGGA 1 cut(s) 681
MscI TGGCCA 2 cut(s) 488, 935
MseI TTAA 3 cut(s) 306, 537, 903
Msp20I TGGCCA 2 cut(s) 488, 935
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 6 cut(s) 75, 90, 132, 234, 325, 682
MunI CAATTG 1 cut(s) 981
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 236, 992
NarI GGCGCC 1 cut(s) 96
NdeI CATATG 1 cut(s) 404
NdeII GATC 3 cut(s) 21, 707, 1112
NlaIII CATG 5 cut(s) 275, 399, 477, 596, 1032
NlaIV GGNNCC 7 cut(s) 97, 479, 480, 565, 733, 883, 1204
NmuCI GTSAC 2 cut(s) 696, 1032
NsiI ATGCAT 1 cut(s) 401
NspI RCATGY 2 cut(s) 275, 596
PaeI GCATGC 1 cut(s) 275
PaqCI CACCTGC 1 cut(s) 757
PceI AGGCCT 1 cut(s) 805
PciI ACATGT 1 cut(s) 592
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 443, 852, 944
PflFI GACNNNGTC 1 cut(s) 313
PkrI GCNGC 2 cut(s) 95, 1012
PleI GAGTC 1 cut(s) 634
PluTI GGCGCC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 634
PpuMI RGGWCCY 2 cut(s) 478, 793
PscI ACATGT 1 cut(s) 592
Psp5II RGGWCCY 2 cut(s) 478, 793
PspEI GGTNACC 2 cut(s) 696, 1032
PspN4I GGNNCC 7 cut(s) 97, 479, 480, 565, 733, 883, 1204
PspPI GGNCC 3 cut(s) 340, 478, 793
PspPPI RGGWCCY 2 cut(s) 478, 793
PsyI GACNNNGTC 1 cut(s) 313
SaqAI TTAA 3 cut(s) 306, 537, 903
SatI GCNGC 2 cut(s) 94, 1011
Sau3AI GATC 3 cut(s) 21, 707, 1112
Sau96I GGNCC 3 cut(s) 340, 478, 793
SchI GAGTC 1 cut(s) 635
SfaNI GCATC 3 cut(s) 400, 408, 1030
SfoI GGCGCC 1 cut(s) 97
SgrAI CRCCGGYG 1 cut(s) 89
SinI GGWCC 3 cut(s) 340, 478, 793
SmlI CTYRAG 2 cut(s) 305, 344
SmoI CTYRAG 2 cut(s) 305, 344
SphI GCATGC 1 cut(s) 275
Sse9I AATT 7 cut(s) 127, 225, 255, 391, 577, 981, 1091
SseBI AGGCCT 1 cut(s) 805
SsiI CCGC 4 cut(s) 9, 93, 877, 929
SspDI GGCGCC 1 cut(s) 95
SspMI CTAG 2 cut(s) 56, 1052
StuI AGGCCT 1 cut(s) 805
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 2 cut(s) 16, 1197
TaiI ACGT 2 cut(s) 296, 336
TaqI TCGA 2 cut(s) 387, 850
TasI AATT 7 cut(s) 127, 225, 255, 391, 577, 981, 1091
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 3 cut(s) 443, 852, 944
Tru1I TTAA 3 cut(s) 306, 537, 903
Tru9I TTAA 3 cut(s) 306, 537, 903
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 2 cut(s) 696, 1032
TseI GCWGC 1 cut(s) 1010
Tsp45I GTSAC 2 cut(s) 696, 1032
TspDTI ATGAA 6 cut(s) 17, 354, 384, 992, 1119, 1152
TspRI CASTG 1 cut(s) 156
Tth111I GACNNNGTC 1 cut(s) 313
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 3 cut(s) 340, 478, 793
XapI RAATTY 2 cut(s) 127, 1091
XceI RCATGY 2 cut(s) 275, 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 987
XspI CTAG 2 cut(s) 56, 1052
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.